Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123061,854,8510AC24.9% 591.2 / 52.9 242T25P (ACC→CCC) yejHpredicted ATP‑dependet helicase
Reads supporting (aligned to +/- strand):  ref base A (159/22);  new base C (0/60);  total (159/83)
Fisher's exact test for biased strand distribution p-value = 1.57e-38
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 281 total aligned reads displayed.

TAGCGGTCTAATAACATCCCTCAAGGGAAAAAGATCC‑ATGGCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGAACTGGTGGCGCAAAACCATGCAAAGTATCAGGCGCTGGGGCTG  >  AP012306/1854702‑1855000
                                                                                                                                                      |                                                                                                                                                     
  gCGGTCTAATAACATCCCTCAAGGGAAAAAGATCC‑ATGGCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAAcc                                                                                                                                                     <  2:2175473/150‑1 (MQ=255)
   cGGTCTAATAACATCCCTCAAGGGAAAAAGATCC‑ATGGCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAAccc                                                                                                                                                    <  1:3885644/150‑1 (MQ=255)
     gTCTAATAACATCCCTCAAGGGAAAAAGATCC‑ATGGCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCt                                                                                                                                                  <  1:2532936/150‑1 (MQ=255)
         aataaCATCCCTCAAGGGAAAAAGATCC‑ATGGCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCg                                                                                                                                              >  2:4248626/1‑150 (MQ=255)
          ataaCATCCCTCAAGGGAAAAAGATCC‑ATGGCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGt                                                                                                                                             >  1:2336074/1‑150 (MQ=255)
              cATCCCTCAAGGGAAAAAGATCC‑ATGGCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATc                                                                                                                                         >  1:1253809/1‑150 (MQ=255)
                 cccTCAAGGAAAAAAGATCC‑ATGGCATACTATTACCAGAATAATCTCCCTCCGCGAGCCCATGATTTTTCCCCTCCCCCCATATCACCAAGAACCCGTGGATCCCCCCCTCACCCATTTCCCTCCTCATAAACCCCCTGCCGTTATCGtg                                                                                                                                      <  2:2815410/150‑1 (MQ=255)
                    tCAAGGGAAAAAGATCC‑ATGGCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGtgctg                                                                                                                                   >  2:2745766/1‑150 (MQ=255)
                    tCAAGGAAAAAAGATCC‑ATGGCATACTATTAGCAGAATAATCTACCTACGCGAGCCCATGATTTTTAAACTTCGCCCATATCAGCAAGAAGCCGTGGAGGCCACGCTCAACCATTTTCGTCGTCATAAACCCCCTGCCGTTATCGtgctg                                                                                                                                   <  1:798578/150‑1 (MQ=255)
                      aaGGGAAAAAGATCC‑ATGGCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGcc                                                                                                                                 >  1:1407817/1‑150 (MQ=255)
                      aaGGGAAAAAGATCC‑ATGGCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGcc                                                                                                                                 >  1:3662402/1‑150 (MQ=255)
                      aaGGGAAAAAGATCC‑ATGGCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGcc                                                                                                                                 >  1:2715195/1‑150 (MQ=255)
                            aaaaaaTCCAATGCCATCCTATTAGCAAAATAATCCACCTACGCGAGACCATGTTTTTAACCCTCCGCCCATACCCGAAAAAAGCCGTGGTCGCCCCCCAAACCCTTTTCCGTCGAAAAAAACCCCCTCCCGTTATCGTGCTGCCCACCg                                                                                                                            <  2:2617689/150‑1 (MQ=255)
                               aGATCC‑ATGGCATACTATTAGCAGAATAATCTACCTACGCGAAACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACACCTGCCGTTATCGTGCTGCCCACCGgcgc                                                                                                                        >  2:3068046/1‑150 (MQ=255)
                                  tCC‑ATGGCATACTATTAGCAGAATAATCTACCTACGCGAGCCCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCAAAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAgg                                                                                                                     <  2:2925017/150‑1 (MQ=255)
                                  tCC‑ATGACAAACTATTAGATGAATAATCTACCTCCGCGAAACCATGTTTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGAGGCCACGCTCACCAATTTTCGTCGTCAAAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAgg                                                                                                                     <  2:3825268/150‑1 (MQ=255)
                                   cc‑ATGGCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGt                                                                                                                    >  2:679986/1‑150 (MQ=255)
                                    c‑ATGGCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTa                                                                                                                   >  2:1757883/1‑150 (MQ=255)
                                    c‑ATGGCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTa                                                                                                                   >  1:3195354/1‑150 (MQ=255)
                                         gCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAg                                                                                                               >  2:2277227/1‑150 (MQ=255)
                                           aTACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGcc                                                                                                             >  2:543594/1‑150 (MQ=255)
                                           aTACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGcc                                                                                                             <  1:408812/150‑1 (MQ=255)
                                           aTACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGcc                                                                                                             >  1:3697018/1‑150 (MQ=255)
                                            tACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCt                                                                                                            <  1:2252085/150‑1 (MQ=255)
                                            tACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCt                                                                                                            >  2:2562215/1‑150 (MQ=255)
                                            tACTATGAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAACCCt                                                                                                            >  2:3729334/1‑150 (MQ=255)
                                             aCTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTg                                                                                                           >  2:1242456/1‑150 (MQ=255)
                                              cTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTgg                                                                                                          >  2:2417515/1‑150 (MQ=255)
                                              cTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTgg                                                                                                          >  2:2350521/1‑150 (MQ=255)
                                               tATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGt                                                                                                         >  1:4204279/1‑150 (MQ=255)
                                                aTTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTg                                                                                                        >  1:89379/1‑150 (MQ=255)
                                                 ttAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGa                                                                                                       >  1:493766/1‑150 (MQ=255)
                                                 ttAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGa                                                                                                       >  1:860670/1‑150 (MQ=255)
                                                 ttAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGAGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGa                                                                                                       >  2:3395552/1‑150 (MQ=255)
                                                  tAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGAt                                                                                                      >  1:1637950/1‑150 (MQ=255)
                                                  tAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGAt                                                                                                      >  2:1995385/1‑150 (MQ=255)
                                                  aagaaaaatactCTCCCTACGCGAGACAATATTTTTTACCCTCCGCCCATATCAGAAAGAAGCCGTGGATCCCACGCTCACCCATTTTCGTCGTAAAAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGAt                                                                                                      <  2:1022039/144‑1 (MQ=255)
                                                   aGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATa                                                                                                     >  2:3916142/1‑150 (MQ=255)
                                                   aGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATa                                                                                                     >  1:1550235/1‑150 (MQ=255)
                                                   aGCAAAATAATCTACCTACGCGAGACCATAATTTTTACCCTTCGCCCATACCAGAAAGAAGCCGTGGATGCCACGCTCAACTATTTTCGTCGGCATAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAGGCCTGGTGATa                                                                                                     <  2:3567497/150‑1 (MQ=255)
                                                    gCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAg                                                                                                    >  2:2640074/1‑150 (MQ=255)
                                                      aGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCg                                                                                                  >  2:2217831/1‑150 (MQ=255)
                                                        aataatCTCCCTACGCGAGCCCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGAGGCCACGCCCACCCATTTTCGTCGTTAAAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGa                                                                                                <  2:3230974/150‑1 (MQ=255)
                                                        aataatCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGa                                                                                                >  2:3146357/1‑150 (MQ=255)
                                                           aatCTACCTACGAGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACt                                                                                             >  2:4219512/1‑150 (MQ=255)
                                                             tCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTgg                                                                                           >  1:1764636/1‑150 (MQ=255)
                                                               tacctacGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCa                                                                                         <  1:2876307/150‑1 (MQ=255)
                                                               tacctacGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCa                                                                                         >  1:2165271/1‑150 (MQ=255)
                                                                  ctacGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACgg                                                                                      >  2:139800/1‑150 (MQ=255)
                                                                     cgcgAGACCATGATTTTTACACTTCCCCCATACCAGAAAGAACCCGGGGATGCCACGCCCAACCATTTTCGTCGTCATAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACggctg                                                                                   <  2:2251240/150‑1 (MQ=255)
                                                                            ccATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTg                                                                            >  1:2754756/1‑150 (MQ=255)
                                                                              aTGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGt                                                                          <  1:2206242/150‑1 (MQ=255)
                                                                              aTGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGt                                                                          >  2:206404/1‑150 (MQ=255)
                                                                              aTGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGt                                                                          >  1:2916847/1‑150 (MQ=255)
                                                                               tgttttttCCCCTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGCCGTCATAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTc                                                                         <  2:1672569/147‑1 (MQ=255)
                                                                                gATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTcg                                                                        >  2:1135359/1‑150 (MQ=255)
                                                                                 aTTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTcgc                                                                       >  2:3305657/1‑150 (MQ=255)
                                                                                     ttACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCgtgc                                                                   >  2:3991678/1‑150 (MQ=255)
                                                                                       acacTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCgtgctg                                                                 >  2:1843627/1‑150 (MQ=255)
                                                                                          cTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCgtgctggtg                                                              >  2:2111685/1‑150 (MQ=255)
                                                                                          cTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCgtgctggtg                                                              >  1:2623080/1‑150 (MQ=255)
                                                                                           ttCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCTTGGTCGCgtgctggtgc                                                             >  1:3973415/1‑150 (MQ=255)
                                                                                            tCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCgtgctggtgct                                                            <  1:1037621/150‑1 (MQ=255)
                                                                                            ccGCCCATACCCCCAAGACCCCGTGTACCCCCCGCCCACCCATTTTCTTCCTCATAAACCCCCTGCCGTTATCGTGCTGCCCACCGCCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCgtgctggtgct                                                            <  2:2866292/149‑1 (MQ=255)
                                                                                              gCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGtgctggtgctgg                                                          >  1:363302/1‑150 (MQ=255)
                                                                                                    atCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACg                                                    >  1:2506020/1‑150 (MQ=255)
                                                                                                      cAGGAAGAAGCGGGGGATGCCACGCTCAACCTTTTTGGTCGTCAAAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCCCGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGtt                                                  <  2:1513195/150‑1 (MQ=255)
                                                                                                      cAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGtt                                                  >  1:2995591/1‑150 (MQ=255)
                                                                                                        gCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTaa                                                >  2:3509565/1‑150 (MQ=255)
                                                                                                           agaagCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGa                                             >  2:1287207/1‑150 (MQ=255)
                                                                                                            ggagCCGTGGATGCCACCCAAAACCATTTGCGCCTTCAAAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGaa                                            <  2:3328410/148‑1 (MQ=255)
                                                                                                              agCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGAACt                                          <  1:1139739/150‑1 (MQ=255)
                                                                                                                 gggggATGCCACGCTCACCCATTTTCGTCGTCAAAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGAACtggt                                       <  2:638824/147‑1 (MQ=255)
                                                                                                                 cGTGGATGCCACGCTCAACCTTTTTCGTCGTCATAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGAACtggt                                       <  1:3360278/150‑1 (MQ=255)
                                                                                                                 cGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGAACtggt                                       <  1:2451711/150‑1 (MQ=255)
                                                                                                                    ggATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGAACTGGTGgc                                    >  2:852197/1‑150 (MQ=255)
                                                                                                                      aTGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGAACTGGTGgcgc                                  >  2:1061778/1‑150 (MQ=255)
                                                                                                                      aTGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGAACTGGTGgcgc                                  >  1:707087/1‑150 (MQ=255)
                                                                                                                        gCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGAACTGGTGGCGCaa                                >  1:2880124/1‑150 (MQ=255)
                                                                                                                           aCGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGAACTGGTGGCGCAAAAc                             >  1:1480504/1‑150 (MQ=255)
                                                                                                                           aCGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGAACTGGTGGCGCAAAAc                             >  1:303636/1‑150 (MQ=255)
                                                                                                                            cGCTCACCCATTTTCGTCGTCATAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGAACTGCTGGCGCAAAAcc                            <  2:4166867/150‑1 (MQ=255)
                                                                                                                             gCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGAACTGGTGGCGCAAAACCa                           >  1:4195588/1‑150 (MQ=255)
                                                                                                                              cTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGAACTGGTGGCGCAAAACCAt                          >  2:2768266/1‑150 (MQ=255)
                                                                                                                                cAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGAACTGGTGGCGCAAAACCATGc                        >  1:3423099/1‑150 (MQ=255)
                                                                                                                                 aaCCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGAACTGGTGGCGTAAAACCATGCa                       <  1:1866937/150‑1 (MQ=255)
                                                                                                                                   ccATTTTCGTCGTCATAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGAACTGGTGGCGCAAAACCATGCaaa                     <  1:1186985/150‑1 (MQ=255)
                                                                                                                                     aTTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGAACTGGTGGCGCAAAACCATGCAAAGt                   >  1:1993465/1‑150 (MQ=255)
                                                                                                                                       tttCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGAACTGGTGGCGCAAAACCATGCAAAGTAt                 >  1:1334544/1‑150 (MQ=255)
                                                                                                                                          cgtcgtCATAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGAACTGGTGGCGCAAAACCATGCAAAGTATCAg              <  1:189974/150‑1 (MQ=255)
                                                                                                                                             cttcATAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGAACTGGTGGCGCAAAACCATGCAAAGTATCAGgcg           <  2:2435801/148‑1 (MQ=255)
                                                                                                                                               tcATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGAACTGGTGGCGCAAAACCATGCAAAGTATCAGGCGCt         >  1:3758980/1‑150 (MQ=255)
                                                                                                                                               tcATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGAACTGGTGGCGCAAAACCATGCAAAGTATCAGGCGCt         >  1:2865167/1‑150 (MQ=255)
                                                                                                                                                cATAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGAACTGGTGGCGCAAAACCATGCAAAGTATCAGGCGCTg        <  1:3473578/150‑1 (MQ=255)
                                                                                                                                                 aTAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGAACTGGTGGCGCAAAACCATGCAAAGTATCAGGCGCTgg       >  2:2020705/1‑150 (MQ=255)
                                                                                                                                                 aTAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGAACTGGTGGCGCAAAACCATGCAAAGTATCAGGCGCTgg       >  2:3019861/1‑150 (MQ=255)
                                                                                                                                                  tAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGAACTGGTGGCGCAAAACCATGCAAAGTATCAGGCGCTggg      <  1:1414861/150‑1 (MQ=255)
                                                                                                                                                  tAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGAACTGGTGGCGCAAAACCATGCAAAGTATCAGGCGCTggg      >  1:2053186/1‑150 (MQ=255)
                                                                                                                                                  tAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGAACTGGTGGCGCAAAACCATGCAAAGTATCAGGCGCTggg      >  2:4098855/1‑150 (MQ=255)
                                                                                                                                                   aaaaCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGAACTGGTGGCGCAAAACCATGCAAAGTATCAGGCGCTgggg     >  2:3130780/1‑150 (MQ=255)
                                                                                                                                                      |                                                                                                                                                     
TAGCGGTCTAATAACATCCCTCAAGGGAAAAAGATCC‑ATGGCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGAACTGGTGGCGCAAAACCATGCAAAGTATCAGGCGCTGGGGCTG  >  AP012306/1854702‑1855000

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑