BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AP012306	1,156,359	G→T	P169T (CCA→ACA)	sapF ←	predicted antimicrobial peptide transporter subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AP012306	1,156,359	0	G	T	100.0%	1122.1 / NA	275	P169T (CCA→ACA)	sapF	predicted antimicrobial peptide transporter subunit
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (154/121); total (154/121)

Only 100 of 280 total aligned reads displayed.

TAATGTGCTTCATCATTCCGATATGCTGGGTAACATAAATATACGAAATGCCCTGTTTTTCCTGTAATTCCAGCATCAGATTAATCAACTGCGAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCTGTTCCGGTTCCAGGT  >  AP012306/1156212‑1156505

  aTGTGCTTCATCATTCCGATATGCTGGGTAACATAAATATACGAAATGCCCTGTTTTTCCTGTAATTCCAGCATCAGATTAATCAACTGCGAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGTgcgc                                                                                                                                                >  2:3650775/1‑150 (MQ=255)

  aTGTGCTTCATCATTCCGATATGCTGGGTAACATAAATATACGAAATGCCCTGTTTTTCCTGTAATTCCAGCATCAGATTAATCAACTGCGAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGTgcgc                                                                                                                                                >  1:2181558/1‑150 (MQ=255)

     tgCTTCATCATTCCGATATGCTGGGTAACATAAATATACGAAATGCCCTGTTTTTCCTGTAATTCCAGCATCAGATTAATCAACTGCGAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGTGCGCAat                                                                                                                                             >  1:1043556/1‑150 (MQ=255)

     tgCTTCATCATTCCGATATGCTGGGTAACATAAATATACGAAATGCCCTGTTTTTCCTGTAATTCCAGCATCAGATTAATCAACTGCGAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGTGCGCAat                                                                                                                                             >  2:1564035/1‑150 (MQ=255)

     tgCTTCATCATTCCGATATGCTGGGTAACATAAATATACGAAATGCCCTGTTTTTCCTGTAATTCCAGCATCAGATTAATCAACTGCGAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGTGCGCAat                                                                                                                                             <  1:2455267/150‑1 (MQ=255)

      gCTTCATCATTCCGATATGCTGGGTAACATAAATATACGAAATGCCCTGTTTTTCCTGTAATTCCAGCATCAGATTAATCAACTGCGAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATTACTTTTGTGCGCAata                                                                                                                                            >  2:1470157/1‑150 (MQ=255)

      gCTTCATCATTCCGATATGCTGGGTAACATAAATATACGAAATGCCCTGTTTTTCCTGTAATTCCAGCATCAGATTAATCAACTGCGAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGTGCGCAata                                                                                                                                            <  1:1725323/150‑1 (MQ=255)

        ttCATCATTCCGATATGCTGGGTAACATAAATATACGAAATGCCCTGTTTTTCCTGTAATTCCAGCATCAGATTAATCAACTGCGAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGTGCGCAATATc                                                                                                                                          >  1:4116396/1‑150 (MQ=255)

         tcatcaTTCCGATATGCTGGGTAACATAAATATACGAAATGCCCTGTTTTTCCTGTAATTCCAGCATCAGATTAATCAACTGCGAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGTGCGCAATATCa                                                                                                                                         <  2:2427278/150‑1 (MQ=255)

           atcatTCCGATATGCTGGGTAACATAAATATACGAAATGCCCTGTTTTTCCTGTAATTCCAGCATCAGATTAATCAACTGCGAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGTGCGCAATATCAgc                                                                                                                                       >  2:4236341/1‑150 (MQ=255)

           atcatTCCGATATGCTGGGTAACATAAATATACGAAATGCCCTGTTTTTCCTGTAATTCCAGCATCAGATTAATCAACTGCGAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGTGCGCAATATCAgc                                                                                                                                       <  1:1736629/150‑1 (MQ=255)

           atcatTCCGATATGCTGGGTAACATAAATATACGAAATGCCCTGTTTTTCCTGTAATTCCAGCATCAGATTAATCAACTGCGAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGTGCGCAATATCAgc                                                                                                                                       >  2:3811183/1‑150 (MQ=255)

             catTCCGATATGCTGGGTAACATAAATATACGAAATGCCCTGTTTTTCCTGTAATTCCAGCATCAGATTAATCAACTGCGAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGTGCGCAATATCAgcgc                                                                                                                                     >  2:36991/1‑150 (MQ=255)

                 ccGATATGCTGGGTAACATAAATATACGAAATGCCCTGTTTTTCCTGTAATTCCAGCATCAGATTAATCAACTGCGAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGTGCGCAATATCAgcgcgcgc                                                                                                                                 >  1:1896874/1‑150 (MQ=255)

                  cGATATGCTGGGTAACATAAATATACGAAATGCCCTGTTTTTCCTGTAATTCCAGCATCAGATTAATCAACTGCGAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGTGCGCAATATCAgcgcgcgcg                                                                                                                                >  2:2220471/1‑150 (MQ=255)

                   gATATGCTGGGTAACATAAATATACGAAATGCCCTGTTTTTCCTGTAATTCCAGCATCAGATTAATCAACTGCGAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGTGCGCAATATCAgcgcgcgcgc                                                                                                                               >  1:1260477/1‑150 (MQ=255)

                        gCTGGGTAACATAAATATACGAAATGCCCTGTTTTTCCTGTAATTCCAGCATCAGATTAATCAACTGCGAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGTGCGCAATATCAGCGCGCGCGccagac                                                                                                                          >  1:205227/1‑150 (MQ=255)

                        gCTGGGTAACATAAATATACGAAATGCCCTGTTTTTCCTGTAATTCCAGCATCAGATTAATCAACTGCGAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGTGCGCAATATCAGCGCGCGCGccagac                                                                                                                          >  2:836085/1‑150 (MQ=255)

                         cTGGGTAACATAAATATACGAAATGCCCTGTTTTTCCTGTAATTCCAGCATCAGATTAATCAACTGCGAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGTGCGCAATATCAGCGCGCGCGccagacc                                                                                                                         >  2:1374303/1‑150 (MQ=255)

                           gggTAACATAAATATACGAAATGCCCTGTTTTTCCTGTAATTCCAGCATCAGATTAATCAACTGCGAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGTGCGCAATATCAGCGCGCGCGccagaccca                                                                                                                       <  1:3094644/150‑1 (MQ=255)

                               aaCATAAATATACGAAATGCCCTGTTTTTCCTGTAATTCCAGCATCAGATTAATCAACTGCGAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGTGCGCAATATCAGCGCGCGCGCCAGACCCAGACg                                                                                                                   <  1:2851464/150‑1 (MQ=255)

                                  aTAAATATACGAAATGCCCTGTTTTTCCTGTAATTCCAGCATCAGATTAATCAACTGCGAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGTGCGCAATATCAGCGCGCGCGCCAGACCCAGACGCTg                                                                                                                >  1:3765623/1‑150 (MQ=255)

                                    aaaTATACGAAATGCCCTGTTTTTCCTGTAATTCCAGCATCAGATTAATCAACTGCGAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGTGCGCAATATCAGCGCGCGCGCCAGACCCAGACGCTGtt                                                                                                              >  2:4081402/1‑150 (MQ=255)

                                      atatACGAAATGCCCTGTTTTTCCTGTAATTCCAGCATCAGATTAATCAACTGCGAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGTGCGCAATATCAGCGCGCGCGCCAGACCCAGACGCTGtttt                                                                                                            >  2:3365752/1‑150 (MQ=255)

                                      atatACGAAATGCCCTGTTTTTCCTGTAATTCCAGCATCAGATTAATCAACTGCGAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGTGCGCAATATCAGCGCGCGCGCCAGACCCAGACGCTGtttt                                                                                                            >  2:3570822/1‑150 (MQ=255)

                                        ataCGAAATGCCCTGTTTTTCCTGTAATTCCAGCATCAGATTAATCAACTGCGAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGTGCGCAATATCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTg                                                                                                          >  1:1119717/1‑150 (MQ=255)

                                        ataCGAAATGCCCTGTTTTTCCTGTAATTCCAGCATCAGATTAATCAACTGCGAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGTGCGCAATATCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTg                                                                                                          <  1:2293887/150‑1 (MQ=255)

                                            gAAATGCCCTGTTTTTCCTGTAATTCCAGCATCAGATTAATCAACTGCGAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGTGCGCAATATCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGTCCg                                                                                                      >  1:613647/1‑150 (MQ=255)

                                             aaaTGCCCTGTTTTTCCTGTAATTCCAGCATCAGATTAATCAACTGCGAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGTGCGCAATATCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGTCCgg                                                                                                     <  1:3060648/150‑1 (MQ=255)

                                                    cTGTTTTTCCTGTAATTCCAGCATCAGATTAATCAACTGCGAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGTGCGCAATATCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGTCCGGGTGCCaa                                                                                              <  1:1012024/150‑1 (MQ=255)

                                                     tGTTTTTCCTGTAATTCCAGCATCAGATTAATCAACTGCGAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGTGCGCAATATCAGCGCGCGCGCCAGACCCAGATGCTGTTTTTGTCCGGGTGCCAAc                                                                                             <  1:1465124/150‑1 (MQ=255)

                                                      gTTTTTCCTGTAATTCCAGCATCAGATTAATCAACTGCGAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGTGCGCAATATCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGTCCGGGTGCCAACa                                                                                            >  2:2753259/1‑150 (MQ=255)

                                                      gTTTTTCCTGTAATTCCAGCATCAGATTAATCAACTGCGAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGTGCGCAATATCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGTCCGGGTGCCAACa                                                                                            <  1:3492271/150‑1 (MQ=255)

                                                        ttttCCTGTAATTCCAGCATCAGATTAATCAACTGCGAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGTGCGCAATATCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGTCCGGGTGCCAACata                                                                                          <  1:4249027/150‑1 (MQ=255)

                                                        ttttCCTGTAATTCCAGCATCAGATTAATCAACTGCGAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGTGCGCAATATCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGTCCGGGTGCCAACata                                                                                          >  2:4135451/1‑150 (MQ=255)

                                                         tttCCTGTAATTCCAGCATCAGATTAATCAACTGCGAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGTGCGCAATATCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGTCCGGGTGCCAACatat                                                                                         >  2:418047/1‑150 (MQ=255)

                                                         tttCCTGTAATTCCAGCATCAGATTAATCAACTGCGAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGTGCGCAATATCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGTCCGGGTGCCAACatat                                                                                         <  2:1812148/150‑1 (MQ=255)

                                                         tttCCTGTAATTCCAGCATCAGATTAATCAACTGCGAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGTGCGCAATATCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGTCCGGGTGCCAACatat                                                                                         <  1:3732174/150‑1 (MQ=255)

                                                          ttCCTGTAATTCCAGCATCAGATTAATCAACTGCGAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGTGCGCAATATCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGTCCGGGTGCCAACATATg                                                                                        <  1:2141297/150‑1 (MQ=255)

                                                            ccTGTAATTCCAGCATCAGATTAATCAACTGCGAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGTGCGCAATATCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGTCCGGGTGCCAACATATGCg                                                                                      <  1:3300366/150‑1 (MQ=255)

                                                                tAATTCCAGCATCAGATTAATCAACTGCGAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGTGCGCAATATCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGTCCGGGTGCCAACATATGCGGATa                                                                                  <  2:516796/150‑1 (MQ=255)

                                                                 aaTTCCAGCATCAGATTAATCAACTGCGAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGTGCGCAATATCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGTCCGGGTGCCAACATATGCGGATAg                                                                                 >  1:2028267/1‑150 (MQ=255)

                                                                    tCCAGCATCAGATTAATCAACTGCGAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGTGCGCAATATCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTaa                                                                              >  2:2113548/1‑150 (MQ=255)

                                                                    tCCAGCATCAGATTAATCAACTGCGAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGTGCGCAATATCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTaa                                                                              <  2:682561/150‑1 (MQ=255)

                                                                    tCCAGCATCAGATTAATCAACTGCGAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGTGCGCAATATCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTaa                                                                              >  1:685551/1‑150 (MQ=255)

                                                                     ccAGCATCAGATTAATCAACTGCGAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGTGCGCAATATCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAAc                                                                             <  2:2555179/150‑1 (MQ=255)

                                                                      cAGCATCAGATTAATCAACTGCGAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGTGCGCAATATCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACt                                                                            >  1:1885052/1‑150 (MQ=255)

                                                                       aGCATCAGATTAATCAACTGCGAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGTGCGCAATATCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTg                                                                           >  2:2344891/1‑150 (MQ=255)

                                                                        gCATCAGATTAATCAACTGCGAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGTGCGCAATATCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGa                                                                          >  1:2705033/1‑150 (MQ=255)

                                                                              gATTAATCAACTGCGAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGTGCGCAATATCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGAt                                                                    >  2:3945623/1‑150 (MQ=255)

                                                                              gATTAATCAACTGCGAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGTGCGCAATATCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGTCCGGGTGCCAACATATGCAGATAGTAACTGACGTGAt                                                                    >  1:2227020/1‑150 (MQ=255)

                                                                                ttAATCAACTGCGAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGTGCGCAATATCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATcc                                                                  >  1:4225510/1‑150 (MQ=255)

                                                                                 tAATCAACTGCAAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGTGCGCAATATCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCg                                                                 >  1:2986563/1‑150 (MQ=255)

                                                                                      aaCTGCGAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGTGCGCAATATCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTagc                                                            <  1:490698/150‑1 (MQ=255)

                                                                                       aCTGCGAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGTGCGCAATATCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTagca                                                           >  2:3321860/1‑150 (MQ=255)

                                                                                        cTGCGAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGTGCGCAATATCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTagcag                                                          <  1:339180/150‑1 (MQ=255)

                                                                                        cTGCGAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGTGCGCAATATCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTagcag                                                          >  1:810360/1‑150 (MQ=255)

                                                                                          gCGAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGTGCGCAATATCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGcc                                                        <  1:2738610/150‑1 (MQ=255)

                                                                                               cGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGTGCGCAATATCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAAcc                                                   >  2:578056/1‑150 (MQ=255)

                                                                                                gCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGTGCGCAATATCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCa                                                  >  1:414402/1‑150 (MQ=255)

                                                                                                gCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGTGCGCAATATCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCa                                                  >  2:3188293/1‑150 (MQ=255)

                                                                                                  aTCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGTGCGCAATATCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATa                                                <  2:447657/150‑1 (MQ=255)

                                                                                                  aTCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGTGCGCAATATCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATa                                                >  1:3815884/1‑150 (MQ=255)

                                                                                                    cGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGTGCGCAATATCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACg                                              <  1:1598926/150‑1 (MQ=255)

                                                                                                     gACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGTGCGCAATATCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGc                                             >  2:2053748/1‑150 (MQ=255)

                                                                                                        atatCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGTGCGCAATATCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATc                                          >  1:1516120/1‑150 (MQ=255)

                                                                                                          atCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGTGCGCAATATCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGt                                        >  2:1873349/1‑150 (MQ=255)

                                                                                                               gTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGTGCGCAATATCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCaa                                   >  2:2611527/1‑150 (MQ=255)

                                                                                                                  aaGCGAGGGCTTCATCGGCAATAATGACTTTTGTGCGCAATATCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCaataa                                >  1:980925/1‑150 (MQ=255)

                                                                                                                  aaGCGAGGGCTTCATCGGCAATAATGACTTTTGTGCGCAATATCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCaataa                                <  2:1119845/150‑1 (MQ=255)

                                                                                                                   aGCGAGGGCTTCATCGGCAATAATGACTTTTGTGCGCAATATCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCaataat                               <  1:2427939/150‑1 (MQ=255)

                                                                                                                     cGAGGGCTTCATCGGCAATAATGACTTTTGTGCGCAATATCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCt                             <  1:3951240/150‑1 (MQ=255)

                                                                                                                     cGAGGGCTTCATCGGCAATAATGACTTTTGTGCGCAATATCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCt                             >  2:3265302/1‑150 (MQ=255)

                                                                                                                        gggCTTCATCGGCAATAATGACTTTTGTGCGCAATATCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGtt                          <  1:1947853/150‑1 (MQ=255)

                                                                                                                         ggCTTCATCGGCAATAATGACTTTTGTGCGCAATATCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGttt                         >  1:916179/1‑150 (MQ=255)

                                                                                                                           cTTCATCGGCAATAATGACTTTTGTGCGCAATATCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGc                       >  1:1862980/1‑150 (MQ=255)

                                                                                                                           cTTCATCGGCAATAATGACTTTTGTGCGCAATATCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGc                       >  2:3033967/1‑150 (MQ=255)

                                                                                                                            ttCATCGGCAATAATGACTTTTGTGCGCAATATCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCg                      >  2:2334869/1‑150 (MQ=255)

                                                                                                                            ttCATCGGCAATAATGACTTTTGTGCGCAATATCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCg                      >  2:1219774/1‑150 (MQ=255)

                                                                                                                            ttCATCGGCAATAATGACTTTTGTGCGCAATATCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCg                      <  2:1967489/150‑1 (MQ=255)

                                                                                                                                 cGGCAATAATGACTTTTGTGCGCAATATCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCt                 <  1:2626408/150‑1 (MQ=255)

                                                                                                                                 cGGCAATAATGACTTTTGTGCGCAATATCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGATAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCt                 <  1:420979/150‑1 (MQ=255)

                                                                                                                                   gCAATAATGACTTTTGTGCGCAATATCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCTGt               <  1:3646553/150‑1 (MQ=255)

                                                                                                                                   gCAATAATGACTTTTGTGCGCAATATCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCTGt               >  1:390341/1‑150 (MQ=255)

                                                                                                                                    cAATAATGACTTTTGTGCGCAATATCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCTGtt              <  2:1497075/150‑1 (MQ=255)

                                                                                                                                    cAATAATGACTTTTGTGCGCAATATCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCTGtt              <  1:4006210/150‑1 (MQ=255)

                                                                                                                                     aataatGACTTTTGTGCGCAATATCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCTGTTc             >  1:2503472/1‑150 (MQ=255)

                                                                                                                                      ataatGACTTTTGTGCGCAATATCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCTGTTcc            <  2:1656184/150‑1 (MQ=255)

                                                                                                                                       taatGACTTTTGTGCGCAATATCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCTGTTCCg           >  2:918458/1‑150 (MQ=255)

                                                                                                                                       taatGACTTTTGTGCGCAATATCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCTGTTCCg           >  2:1047118/1‑150 (MQ=255)

                                                                                                                                       taatGACTTTTGTGCGCAATATCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCTGTTCCg           >  2:552665/1‑150 (MQ=255)

                                                                                                                                       taatGACTTTTGTGCGCAATATCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCTGTTCCg           >  2:3435626/1‑150 (MQ=255)

                                                                                                                                        aatGACTTTTGTGCGCAATATCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCTGTTCCgg          >  1:2803368/1‑150 (MQ=255)

                                                                                                                                            aCTTTTGTGCGCAATATCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCTGTTCCGGTTcc      <  1:3037334/150‑1 (MQ=255)

                                                                                                                                             cTTTTGTGCGCAATATCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCTGTTCCGGTTCCa     >  1:1370198/1‑150 (MQ=255)

                                                                                                                                             cTTTTGTGCGCAATATCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCTGTTCCGGTTCCa     >  1:1347016/1‑150 (MQ=255)

                                                                                                                                             cTTTTGTGCGCAATATCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCTGTTCCGGTTCCa     <  1:120346/150‑1 (MQ=255)

                                                                                                                                             cTTTTGTGCGCAATATCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGGCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCTGTTCCGGTACCa     <  2:3141075/150‑1 (MQ=255)

                                                                                                                                               tttGTGCGCAATATCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCTGTTCCGGTTCCAgg   <  2:2830822/150‑1 (MQ=255)

                                                                                                                                                ttGTGCGCAATATCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCTGTTCCGGTTCCAGGt  <  1:3410268/150‑1 (MQ=255)

TAATGTGCTTCATCATTCCGATATGCTGGGTAACATAAATATACGAAATGCCCTGTTTTTCCTGTAATTCCAGCATCAGATTAATCAACTGCGAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCTGTTCCGGTTCCAGGT  >  AP012306/1156212‑1156505

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`