Predicted mutation
evidence seq id position mutation annotation gene description
RA AP012306 2,904,141 G→T W361C (TGG→TGT)  rsmB → 16S rRNA m5C967 methyltransferase, S‑adenosyl‑L‑methionine‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123062,904,1410GT100.0% 1503.7 / NA 389W361C (TGG→TGT) rsmB16S rRNA m5C967 methyltransferase, S‑adenosyl‑L‑methionine‑dependent
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (206/183);  total (206/183)

Only 100 of 392 total aligned reads displayed.

CGAGCAACAGTTTGATCGCATTTTATTAGATGCGCCTTGTTCAGCAACCGGTGTGATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCGCAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGCCACCTGTTCGGTGTTACCGGAAGAGAATAGCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCAGAGCAACCGGGTAAACA  >  AP012306/2903994‑2904287
                                                                                                                                                   |                                                                                                                                                  
cGAGCAACAGTTTGATCGCATTTTATTAGATGCGCCTTGTTCAGCAACCGGTGTGATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCGCAATTGCAGTCTGAAATTCTCGACGCCATTTGTcc                                                                                                                                                  >  1:2878841/1‑150 (MQ=255)
cGAGCAACAGTTTGATCGCATTTTATTAGATGCGCCTTGTTCAGCAACCGGTGTGATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCGCAATTGCAGTCTGAAATTCTCGACGCCATTTGTcc                                                                                                                                                  >  2:4206388/1‑150 (MQ=255)
  aGCAACAGTTTGATCGCATTTTATTAGATGCGCCTTGTTCAGCAACCGGTGTGATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCGCAATTGCAGTCTGAAATTCTCGACGCCATTTGTCCGc                                                                                                                                                <  2:3887522/150‑1 (MQ=255)
   gCAACAGTTTGATCGCATTTTATTAGATGCGCCTTGTTCAGCAACCGGTGTGATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCGCAATTGCAGTCTGAAATTCTCGACGCCATTTGTCCGCa                                                                                                                                               >  2:2435649/1‑150 (MQ=255)
    cAACAGTTTGATCGCATTTTATTAGATGCGCCTTGTTCAGCAACCGGTGTGATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCGCAATTGCAGTCTGAAATTCTCGACGCCATTTGTCCGCAt                                                                                                                                              >  1:721181/1‑150 (MQ=255)
    cAACAGTTTGATCGCATTTTATTAGATGCGCCTTGTTCAGCAACCGGTGTGATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCGCAATTGCAGTCTGAAATTCTCGACGCCATTTGTCCGCAt                                                                                                                                              >  2:1429688/1‑150 (MQ=255)
     aaCAGTTTGATCGCATTTTATTAGATGCGCCTTGTTCAGCAACCGGTGTGATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCGCAATTGCAGTCTGAAATTCTCGACGCCATTTGTCCGCAtt                                                                                                                                             >  2:863420/1‑150 (MQ=255)
      aCAGTTTGATCGCATTTTATTAGATGCGCCTTGTTCAGCAACCGGTGTGATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCGCAATTGCAGTCTGAAATTCTCGACGCCATTTGTCCGCAttt                                                                                                                                            <  2:3827537/150‑1 (MQ=255)
      aCAGTTTGATCGCATTTTATTAGATGCGCCTTGTTCAGCAACCGGTGTGATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCGCAATTGAAGTCTGAAATTCTCGACGCCATTTGTCCGCAttt                                                                                                                                            <  1:3520162/150‑1 (MQ=255)
        aGTTTGATCGCATTTTATTAGATGCGCCTTGTTCAGCAACCGGTGTGATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCGCAATTGCAGTCTGAAATTCTCGACGCCATTTGTCCGCATTTaa                                                                                                                                          <  2:3380107/150‑1 (MQ=255)
        aGTTTGATCGCATTTTATTAGATGCGCCTTGTTCAGCAACCGGTGTGATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCGCAATTGCAGTCTGAAATTCTCGACGCCATTTGTCCGCATTTaa                                                                                                                                          >  1:2664530/1‑150 (MQ=255)
          tttGATCGCATTTTATTAGATGCGCCTTGTTCAGCAACCGGTGTGATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCGCAATTGCAGTCTGAAATTCTCGACGCCATTTGTCCGCATTTaaaa                                                                                                                                        <  2:2701626/150‑1 (MQ=255)
          tttGATCGCATTTTATTAGATGCGCCTTGTTCAGCAACCGGTGTGATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCGCAATTGCAGTCTGAAATTCTCGACGCCATTTGTCCGCATTTaaaa                                                                                                                                        <  1:1597282/150‑1 (MQ=255)
                cGCATTTTATTAGATGCGCCTTGTTCAGCAACCGGTGTGATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCGCAATTGCAGTCTGAAATTCTCGACGCCATTTGTCCGCATTTAAAAACCGGt                                                                                                                                  <  2:2634484/150‑1 (MQ=255)
                 gCATTTTATTAGATGCGCCTTGTTCAGCAACCGGTGTGATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCGCAATTGCAGTCTGAAATTCTCGACGCCATTTGTCCGCATTTAAAAACCGGTg                                                                                                                                 >  1:2065990/1‑150 (MQ=255)
                 gCATTTTATTAGATGCGCCTTGTTCAGCAACCGGTGTGATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCGCAATTGCAGTCTGAAATTCTCGACGCCATTTGTCCGCATTTAAAAACCGGTg                                                                                                                                 >  1:3062900/1‑150 (MQ=255)
                   aTTTTATTAGATGCGCCTTGTTCAGCAACCGGTGTGATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCGCAATTGCAGTCTGAAATTCTCGACGCCATTTGTCCGCATTTAAAAACCGGTGGa                                                                                                                               >  1:3511328/1‑150 (MQ=255)
                     tttATTAGATGCGCCTTGTTCAGCAACCGGTGTGATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCGCAATTGCAGTCTGAAATTCTCGACGCCATTTGTCCGCATTTAAAAACCGGTGGAAc                                                                                                                             >  1:3720677/1‑150 (MQ=255)
                      ttattaGATGCGCCTTGTTCAGCAACCGGTGTGATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCGCAATTGCAGTCTGAAATTCTCGACGCCATTTGTCCGCATTTAAAAACCGGTGGAAct                                                                                                                            <  1:3598976/150‑1 (MQ=255)
                        attaGATGCGCCTTGTTCAGCAACCGGTGTGATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCGCAATTGCAGTCTGAAATTCTCGACGCCATTTGTCCGCATTTAAAAACCGGTGGAActct                                                                                                                          >  1:664870/1‑150 (MQ=255)
                        attaGATGCGCCTTGTTCAGCAACCGGTGTGATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCGCAATTGCAGTCTGAAATTCTCGACGCCATTTGTCCGCATTTAAAAACCGGTGGAActct                                                                                                                          <  1:520314/150‑1 (MQ=255)
                        attaGATGCGCCTTGTTCAGCAACCGGTGTGATTCGTCGCCATCCAGATAGTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCGCAATTGCAGTCTGAAATTCTCGACGCCATTTGTCCGCATTTAAAAACCGGTGGAActct                                                                                                                          <  1:830740/150‑1 (MQ=255)
                         ttaGATGCGCCTTGTTCAGCAACCGGTGTGATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCGCAATTGCAGTCTGAAATTCTCGACGCCATTTGTCCGCATTTAAAAACCGGTGGAACTCTg                                                                                                                         >  2:3181204/1‑150 (MQ=255)
                           aGATGCGCCTTGTTCAGCAACCGGTGTGATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCGCAATTGCAGTCTGAAATTCTCGACGCCATTTGTCCGCATTTAAAAACCGGTGGAACTCTGGt                                                                                                                       >  1:2405518/1‑150 (MQ=255)
                             aTGCGCCTTGTTCAGCAACCGGTGTGATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCGCAATTGCAGTCTGAAATTCTCGACGCCATTTGTCCGCATTTAAAAACCGGTGGAACTCTGGTCt                                                                                                                     <  1:2201544/150‑1 (MQ=255)
                               gcgcCTTGTTCAGCAACCGGTGTGATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCGCAATTGCAGTCTGAAATTCTCGACGCCATTTGTCCGCATTTAAAAACCGGTGGAACTCTGGTCTAt                                                                                                                   >  1:1932176/1‑150 (MQ=255)
                                  ccTTGTTCAGCAACCGGTGTGATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCGCAATTGCAGTCTGAAATTCTCGACGCCATTTGTCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGcc                                                                                                                >  2:543017/1‑150 (MQ=255)
                                  ccTTGTTCAGCAACCGGTGTGATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCGCAATTGCAGTCTGAAATTCTCGACGCCATTTGTCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGcc                                                                                                                <  2:2534236/150‑1 (MQ=255)
                                     tGTTCAGCAACCGGTGTGATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCGCAATTGCAGTCTGAAATTCTCGACGCCATTTGTCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGCCAcc                                                                                                             >  2:2338004/1‑150 (MQ=255)
                                          aGCAACCGGTGTGATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCGCAATTGCAGTCTGAAATTCTCGACGCCATTTGTCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGCCACCTGTTc                                                                                                        >  2:1200615/1‑150 (MQ=255)
                                             aaCCGGTGTGATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCGCAATTGCAGTCTGAAATTCTCGACGCCATTTGTCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGCCACCTGTTCGgt                                                                                                     >  2:3047859/1‑150 (MQ=255)
                                              aCCGGTGTGATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCGCAATTGCAGTCTGAAATTCTCGACGCCATTTGTCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGCCACCTGTTCGgtg                                                                                                    >  2:1817916/1‑150 (MQ=255)
                                              aCCGGTGTGATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCGCAATTGCAGTCTGAAATTCTCGACGCCATTTGTCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGCCACCTGTTCGgtg                                                                                                    <  2:1486532/150‑1 (MQ=255)
                                                cGGTGTGATTCGTAGCCATCAAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCGCAATTGCAGTCTGAAATTCTCGACGCCATTTGTCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGCCACCTGTTCGGTGtt                                                                                                  >  2:4096492/1‑150 (MQ=255)
                                                 ggTGTGATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCGCAATTGCAGTCTGAAATTCTCGACGCCATTTGTCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGCCACCTGTTCGGTGTTa                                                                                                 >  1:3140273/1‑150 (MQ=255)
                                                 ggTGTGATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCGCAATTGCAGTCTGAAATTCTCGACGCCATTTGTCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGCCACCTGTTCGGTGTTa                                                                                                 <  2:1156121/150‑1 (MQ=255)
                                                  gtgtGATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCGCAATTGCAGTCTGAAATTCTCGACGCCATTTGTCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGCCACCTGTTCGGTGTTAc                                                                                                >  1:142104/1‑150 (MQ=255)
                                                  gtgtGATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCGCAATTGCAGTCTGAAATTCTCGACGCCATTTGTCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGCCACCTGTTCGGTGTTAc                                                                                                >  1:1883298/1‑150 (MQ=255)
                                                    gtgATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCGCAATTGCAGTCTGAAATTCTCGACGCCATTTGTCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGCCACCTGTTCGGTGTTACCg                                                                                              >  1:263363/1‑150 (MQ=255)
                                                      gATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCGCAATTGCAGTCTGAAATTCTCGACGCCATTTGTCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGCCACCTGTTCGGTGTTACCGGa                                                                                            <  1:1744877/150‑1 (MQ=255)
                                                       aTTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCGCAATTGCAGTCTGAAATTCTCGACGCCATTTGTCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGCCACCTGTTCGGTGTTACCGGaa                                                                                           <  2:2497078/150‑1 (MQ=255)
                                                       aTTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCGCAATTGCAGTCTGAAATTCTCGACGCCATTTGTCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGCCACCTGTTCGGTGTTACCGGaa                                                                                           >  1:2477895/1‑150 (MQ=255)
                                                         tcgtcgCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCGCAATTGCAGTCTGAAATTCTCGACGCCATTTGTCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGCCACCTGTTCGGTGTTACCGGAaga                                                                                         <  2:2046426/150‑1 (MQ=255)
                                                          cgtcgCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCGCAATTGCAGTCTGAAATTCTCGACGCCATTTGTCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGCCACCTGTTCGGTGTTACCGGAagag                                                                                        >  1:1258349/1‑150 (MQ=255)
                                                              gCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCGCAATTGCAGTCTGAAATTCTCGACGCCATTTGTCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGCCACCTGTTCGGTGTTACCGGAAGAGAATa                                                                                    >  2:3550313/1‑150 (MQ=255)
                                                               ccATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCGCAATTGCAGTCTGAAATTCTCGACGCCATTTGTCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGCCACCTGTTCGGTGTTACCGGAAGAGAATAg                                                                                   >  2:1301381/1‑150 (MQ=255)
                                                               ccATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCGCAATTGCAGTCTGAAATTCTCGACGCCATTTGTCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGCCACCTGTTCGGTGTTACCGGAAGAGAATAg                                                                                   >  1:847962/1‑150 (MQ=255)
                                                                cATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCGCAATTGCAGTCTGAAATTCTCGACGCCATTTGTCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGCCACCTGTTCGGTGTTACCGGAAGAGAATAGc                                                                                  >  2:1912452/1‑150 (MQ=255)
                                                                cATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCGCAATTGCAGTCTGAAATTCTCGACGCCATTTGTCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGCCACCTGTTCGGTGTTACCGGAAGAGAATAGc                                                                                  >  2:494641/1‑150 (MQ=255)
                                                                  tCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCGCAATTGCAGTCTGAAATTCTCGACGCCATTTGTCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGCCACCTGTTCGGTGTTACCGGAAGAGAATAGCCt                                                                                <  2:2134906/150‑1 (MQ=255)
                                                                   ccAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCGCAATTGCAGTCTGAAATTCTCGACGCCATTTGTCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGCCACCTGTTCGGTGTTACCGGAAGAGAATAGCCTg                                                                               >  1:1784429/1‑150 (MQ=255)
                                                                       atatTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCGCAATTGCAGTCTGAAATTCTCGACGCCATTTGTCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGCCACCTGTTCGGTGTTACCGGAAGAGAATAGCCTGCAGa                                                                           >  2:4264657/1‑150 (MQ=255)
                                                                         atTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCGCAATTGCAGTCTGAAATTCTCGACGCCATTTGTCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGCCACCTGTTCGGTGTTACCGGAAGAGAATAGCCTGCAGAtt                                                                         >  1:419469/1‑150 (MQ=255)
                                                                         atTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCGCAATTGCAGTCTGAAATTCTCGACGCCATTTGTCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGCCACCTGTTCGGTGTTACCGGAAGAGAATAGCCTGCAGAtt                                                                         >  1:3373132/1‑150 (MQ=255)
                                                                            aaaTGGTTACGTCGCGATCGCGATATCCCGGAACTCGCGCAATTGCAGTCTGAAATTCTCGACGCCATTTGTCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGCCACCTGTTCGGTGTTACCGGAAGAGAATAGCCTGCAGATTaaa                                                                      <  1:3972732/150‑1 (MQ=255)
                                                                            aaaTGGTTACGTCGCGATCGCGATATCCCGGAACTCGCGCAATTGCAGTCTGAAATTCTCGACGCCATTTGTCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGCCACCTGTTCGGTGTTACCGGAAGAGAATAGCCTGCAGATTaaa                                                                      <  2:2719338/150‑1 (MQ=255)
                                                                            aaaTGGTTACGTCGCGATCGCGATATCCCGGAACTCGCGCAATTGCAGTCTGAAATTCTCGACGCCATTTGTCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGCCACCTGTTCGGTGTTACCGGAAGAGAATAGCCTGCAGATTaaa                                                                      <  2:103125/150‑1 (MQ=255)
                                                                             aaTGGTTACGTCGCGATCGCGATATCCCGGAACTCGCGCAATTGCAGTCTGAAATTCTCGACGCCATTTGTCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGCCACCTGTTCGGTGTTACCGGAAGAGAATAGCCTGCAGATTAAAg                                                                     <  2:3266559/150‑1 (MQ=255)
                                                                              aTGGTTACGTCGCGATCGCGATATCCCGGAACTCGCGCAATTGCAGTCTGAAATTCTCGACGCCATTTGTCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGCCACCTGTTCGGTGTTACCGGAAGAGAATAGCCTGCAGATTAAAGc                                                                    <  2:719991/150‑1 (MQ=255)
                                                                                 gTTACGTCGCGATCGCGATATCCCGGAACTCGCGCAATTGCAGTCTGAAATTCTCGACGCCATTTGTCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGCCACCTGTTCGGTGTTACCGGAAGAGAATAGCCTGCAGATTAAAGCCtt                                                                 >  1:3313148/1‑150 (MQ=255)
                                                                                  ttACGTCGCGATCGCGATATCCCGGAACTCGCGCAATTGCAGTCTGAAATTCTCGACGCCATTTGTCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGCCACCTGTTCGGTGTTACCGGAAGAGAATAGCCTGCAGATTAAAGCCttt                                                                <  2:590088/150‑1 (MQ=255)
                                                                                   tACGTCGCGATCGCGATATCCCGGAACTCGCGCAATTGCAGTCTGAAATTCTCGACGCCATTTGTCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGCCACCTGTTCGGTGTTACCGGAAGAGAATAGCCTGCAGATTAAAGCCtttt                                                               <  1:2828908/150‑1 (MQ=255)
                                                                                    aCGTCGCGATCGCGATATCCCGGAACTCGCGCAATTGCAGTCTGAAATTCTCGACGCCATTTGTCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGCCACCTGTTCGGTGTTACCGGAAGAGAATAGCCTGCAGATTAAAGCCttttt                                                              >  1:2280276/1‑150 (MQ=255)
                                                                                     cGTCGCGATCGCGATATCCCGGAACTCGCGCAATTGCAGTCTGAAATTCTCGACGCCATTTGTCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGCCACCTGTTCGGTGTTACCGGAAGAGAATAGCCTGCAGATTAAAGCCTTTTTg                                                             <  2:3296124/150‑1 (MQ=255)
                                                                                       tcgcgatcgcgaTATCCCGGAACTCGCGCAATTGCAGTCTGAAATTCTCGACGCCATTTGTCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGCCACCTGTTCGGTGTTACCGGAAGAGAATAGCCTGCAGATTAAAGCCTTTTTGCa                                                           <  1:3775460/150‑1 (MQ=255)
                                                                                         gcgatcgcgatATCCCGGAACTCGCGCAATTGCAGTCTGAAATTCTCGACGCCATTTGTCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGCCACCTGTTCGGTGTTACCGGAAGAGAATAGCCTGCAGATTAAAGCCTTTTTGCAAc                                                         <  1:1093007/150‑1 (MQ=255)
                                                                                          cgatcgcgatATCCCGGAACTCGCGCAATTGCAGTCTGAAATTCTCGACGCCATTTGTCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGCCACCTGTTCGGTGTTACCGGAAGAGAATAGCCTGCAGATTAAAGCCTTTTTGCAAc                                                         <  2:3596215/149‑1 (MQ=255)
                                                                                           gatcgcgatATCCCGGAACTCGCGCAATTGCAGTCTGAAATTCTCGACGCCATTTGTCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGCCACCTGTTCGGTGTTACCGGAAGAGAATAGCCTGCAGATTAAAGCCTTTTTGCAACGt                                                       >  2:174864/1‑150 (MQ=255)
                                                                                           gatcgcgatATCCCGGAACTCGCGCAATTGCAGTCTGAAATTCTCGACGCCATTTGTCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGCCACCTGTTCGGTGTTACCGGAAGAGAATAGCCTGCAGATTAAAGCCTTTTTGCAACGt                                                       >  1:4113735/1‑150 (MQ=255)
                                                                                           gatcgcgatATCCCGGAACTCGCGCAATTGCAGTCTGAAATTCTCGACGCCATTTGTCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGCCACCTGTTCGGTGTTACCGGAAGAGAATAGCCTGCAGATTAAAGCCTTTTTGCAACGt                                                       >  1:4266613/1‑150 (MQ=255)
                                                                                           gatcgcgatATCCCGGAACTCGCGCAATTGCAGTCTGAAATTCTCGACGCCATTTGTCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGCCACCTGTTCGGTGTTACCGGAAGAGAATAGCCTGCAGATTAAAGCCTTTTTGCAACGt                                                       >  1:1028722/1‑150 (MQ=255)
                                                                                           gatcgcgatATCCCGGAACTCGCGCAATTGCAGTCTGAAATTCTCGACGCCATTTGTCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGCCACCTGTTCGGTGTTACCGGAAGAGAATAGCCTGCAGATTAAAGCCTTTTTGCAACGt                                                       <  2:450153/150‑1 (MQ=255)
                                                                                            atcgcgatATCCCGGAACTCGCGCAATTGCAGTCTGAAATTCTCGACGCCATTTGTCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGCCACCTGTTCGGTGTTACCGGAAGAGAATAGCCTGCAGATTAAAGCCTTTTTGCAACGTa                                                      <  2:2843597/150‑1 (MQ=255)
                                                                                            atcgcgatATCCCGGAACTCGCGCAATTGCAGTCTGAAATTCTCGACGCCATTTGTCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGCCACCTGTTCGGTGTTACCGGAAGAGAATAGCCTGCAGATTAAAGCCTTTTTGCAACGTa                                                      >  1:319440/1‑150 (MQ=255)
                                                                                             tcgcgatATCCCGGAACTCGCGCAATTGCAGTCTGAAATTCTCGACGCCATTTGTCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGCCACCTGTTCGGTGTTACCGGAAGAGAATAGCCTGCAGATTAAAGCCTTTTTGCAACGTAc                                                     <  2:3672496/150‑1 (MQ=255)
                                                                                                 gatATCCCGGAACTCGCGCAATTGCAGTCTGAAATTCTCGACGCCATTTGTCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGCCACCTGTTCGGTGTTACCGGAAGAGAATAGCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCt                                                 <  2:2773853/150‑1 (MQ=255)
                                                                                                       ccGGAACTCGCGCAATTGCAGTCTGAAATTCTCGACGCCATTTGTCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGCCACCTGTTCGGTGTTACCGGAAGAGAATAGCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGcc                                           >  1:1645454/1‑150 (MQ=255)
                                                                                                        cGGAACTCGCGCAATTGCAGTCTGAAATTCTCGACGCCATTTGTCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGCCACCTGTTCGGTGTTACCGGAAGAGAATAGCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCg                                          >  1:2247652/1‑150 (MQ=255)
                                                                                                            aCTCGCGCAATTGCAGTCTGAAATTCTCGACGCCATTTGTCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGCCACCTGTTCGGTGTTACCGGAAGAGAATAGCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACt                                      <  2:2973804/150‑1 (MQ=255)
                                                                                                            aCTCGCGCAATTGCAGTCTGAAATTCTCGACGCCATTTGTCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGCCACCTGTTCGGTGTTACCGGAAGAGAATAGCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACt                                      >  1:3032927/1‑150 (MQ=255)
                                                                                                               cgcgCAATTGCAGTCTGAAATTCTCGACGCCATTTGTCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGCCACCTGTTCGGTGTTACCGGAAGAGAATAGCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTg                                   <  1:4005257/150‑1 (MQ=255)
                                                                                                                gcgcAATTGCAGTCTGAAATTCTCGACGCCATTTGTCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGCCACCTGTTCGGTGTTACCGGAAGAGAATAGCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGc                                  >  2:757342/1‑150 (MQ=255)
                                                                                                                    aaTTGCAGTCTGAAATTCTCGACGCCATTTGTCCTCATTTAAAAACCGGTGGAACTCTGGTCTATGCCACCTGTTCGGTGTTACCGGAAGAGAATACCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGaaa                              <  2:1572837/150‑1 (MQ=255)
                                                                                                                            tCTGAAATTCTCGACGCCATTTGTCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGCCACCTGTTCGGTGTTACCGGAAGAGAATAGCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAaca                      <  1:4057054/150‑1 (MQ=255)
                                                                                                                            tCTGAAATTCTCGACGCCATTTGTCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGCCACCTGTTCGGTGTTACCGGAAGAGAATAGCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAaca                      <  1:577222/150‑1 (MQ=255)
                                                                                                                            tCTGAAATTCTCGACGCCATGTGTCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGCCACCTGTTCGGTGTTACCGGAAGAGAATAGCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAaca                      <  1:1340264/150‑1 (MQ=255)
                                                                                                                              tGAAATTCTCGACGCCATTTGTCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGCCACCTGTTCGGTGTTACCGGAAGAGAATAGCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACAcc                    >  2:2021793/1‑150 (MQ=255)
                                                                                                                              tGAAATTCTCGACGCCATTTGTCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGCCACCTGTTCGGTGTTACCGGAAGAGAATAGCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACAcc                    <  2:1649473/150‑1 (MQ=255)
                                                                                                                                aaaTTCTCGACGCCATTTGTCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGCCACCTGTTCGGTGTTACCGGAAGAGAATAGCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCag                  >  2:2257591/1‑150 (MQ=255)
                                                                                                                                 aaTTCTCGACGCCATTTGTCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGCCACCTGTTCGGTGTTACCGGAAGAGAATAGCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCaga                 <  1:99034/150‑1 (MQ=255)
                                                                                                                                      tcGACGCCATTTGTCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGCCACCTGTTCGGTGTTACCGGAAGAGAATAGCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCAGAGCAAc            <  2:629956/150‑1 (MQ=255)
                                                                                                                                       cGACGCCATTTGTCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGCCACCTGTTCGGTGTTACCGGAAGAGAATAGCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCAGAGCAAcc           >  1:324851/1‑150 (MQ=255)
                                                                                                                                        gACGCCATTTGTCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGCCACCTGTTCGGTGTTACCGGAAGAGAATAGCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCAGAGCAACCg          >  1:3877498/1‑150 (MQ=255)
                                                                                                                                         aCGCCATTTGTCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGCCACCTGTTCGGTGTTACCGGAAGAGAATAGCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCAGAGCAACCgg         >  1:1179130/1‑150 (MQ=255)
                                                                                                                                          cGCCATTTGTCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGCCACCTGTTCGGTGTTACCGGAAGAGAATAGCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCaga                 >  1:1195264/1‑141 (MQ=255)
                                                                                                                                           gCCATTTGTCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGCCACCTGTTCGGTGTTACCGGAAGAGAATAGCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCAGAGCAACCGGGt       >  1:169999/1‑150 (MQ=255)
                                                                                                                                           gCCATTTGTCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGCCACCTGTTCGGTGTTACCGGAAGAGAATAGCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCAGAGCAACCGGGt       >  1:1508014/1‑150 (MQ=255)
                                                                                                                                            ccATTTGTCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGCCACCTGTTCGGTGTTACCGGAAGAGAATAGCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCAGAGCAACCGGGTa      <  1:1374315/150‑1 (MQ=255)
                                                                                                                                            ccATTTGTCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGCCACCTGTTCGGTGTTACCGGAAGAGAATAGCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCAGAGCAACCGGGTa      >  2:1383955/1‑150 (MQ=255)
                                                                                                                                              aTTTGTCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGCCACCTGTTCGGTGTTACCGGAAGAGAATAGCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCAGAGCAACCGGGTaaa    >  1:2555077/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CGAGCAACAGTTTGATCGCATTTTATTAGATGCGCCTTGTTCAGCAACCGGTGTGATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCGCAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGCCACCTGTTCGGTGTTACCGGAAGAGAATAGCCTGCAGATTAAAGCCTTTTTGCAACGTACCGCTGATGCCGAACTTTGCGAAACAGGAACACCAGAGCAACCGGGTAAACA  >  AP012306/2903994‑2904287

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑