BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AP012306	189,431	Δ1 bp	coding (89/486 nt)	hlpA →	periplasmic chaperone

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AP012306	189,429	0	G	.	100.0%	1519.2 / NA	304	coding (87/486 nt)	hlpA	periplasmic chaperone
Reads supporting (aligned to +/- strand): ref base G (0/0); new base . (153/151); total (153/151)

Only 100 of 314 total aligned reads displayed.

GATCGCCGGGCCACGCAAAGAACTGCACCCTCCGGTGCAAATGGGATGGTAAGGAGTTTATTGTGAAAAAGTGGTTATTAGCTGCAGGTCTCGGTTTAGCACTGGCAACTTCTGCTCAGGCGGCTGACAAAATTGCAATCGTCAACATGGGCAGCCTGTTCCAGCAGGTAGCGCAGAAAACCGGTGTTTCTAACACGCTGGAAAATGAGTTCAAAGGCCGTGCCAGCGAACTGCAGCGTATGGAAACCGATCTGCAGGCTAAAATGAAAAAGCTGCAGTCCATGAAAGCGGG  >  AP012306/189281‑189572

   cGCCGGGCCACGCAAAGAACTGCACCCTCCGGTGCAAATGGGATGGTAAGGAGTTTATTGTGAAAAAGTGGTTATTAGCTGCAGGTCTCGGTTTAGCACTGGCAACTTCTGCTCAGGCGGCTGACAAAATTGCAATCGTCAACATggcag                                                                                                                                             <  1:2457700/150‑4 (MQ=255)

      cGGGCCACGCAAAGAACTGCACCCTCCGGTGCAAATGGGATGGTAAGGAGTTTATTGTGAAAAAGTGGTTATTAGCTGCAGGTCTCGGTTTAGCACTGGCAACTTCTGCTCAGGCGGCTGACAAAATTGCAATCGTCAACAT‑GGCAGCCt                                                                                                                                         >  2:634859/1‑150 (MQ=255)

       gggCCACGCAAAGAACTGCACCCTCCGGTGCAAATGGGATGGTAAGGAGTTTATTGTGAAAAAGTGGTTATTAGCTGCAGGTCTCGGTTTAGCACTGGCAACTTCTGCTCAGGCGGCTGACAAAATTGCAATCGTCAACAT‑GGCAGCCTg                                                                                                                                        <  2:3488716/150‑1 (MQ=255)

       gggCCACGCAAAGAACTGCACCCTCCGGTGCAAATGGGATGGTAAGGAGTTTATTGTGAAAAAGTGGTTATTAGCTGCAGGTCTCGGTTTAGCACTGGCAACTTCTGCTCAGGCGGCTGACAAAATTGCAATCGTCAACAT‑GGCAGCCTg                                                                                                                                        <  2:3773542/150‑1 (MQ=255)

         gCCACGCAAAGAACTGCACCCTCCGGTGCAAATGGGATGGTAAGGAGTTTATTGTGAAAAAGTGGTTATTAGCTGCAGGTCTCGGTTTAGCACTGGCAACTTCTGCTCAGGCGGCTGACAAAATTGCAATCGTCAACAT‑GGCAGCCTGtt                                                                                                                                      <  1:941684/150‑1 (MQ=255)

           cACGCAAAGAACTGCACCCTCCGGTGCAAATGGGATGGTAAGGAGTTTATTGTGAAAAAGTGGTTATTAGCTGCAGGTCTCGGTTTAGCACTGGCAACTTCTGCTCAGGCGGCTGACAAAATTGCAATCGTCAACAT‑GGCAGCCTGTTcc                                                                                                                                    >  1:1098277/1‑150 (MQ=255)

           cACGCAAAGAACTGCACCCTCCGGTGCAAATGGGATGGTAAGGAGTTTATTGTGAAAAAGTGGTTATTAGCTGCAGGTCTCGGTTTAGCACTGGCAACTTCTGCTCAGGCGGCTGACAAAATTGCAATCGTCAACAT‑GGCAGCCTGTTcc                                                                                                                                    >  1:21661/1‑150 (MQ=255)

              gCAAAGAACTGCACCCTCCGGTGCAAATGGGATGGTAAGGAGTTTATTGTGAAAAAGTGGTTATTAGCTGCAGGTCTCGGTTTAGCACTGGCAACTTCTGCTCAGGCGGCTGACAAAATTGCAATCGTCAACAT‑GGCAGCCTGTTCcagc                                                                                                                                 >  1:475172/1‑150 (MQ=255)

              gCAAAGAACTGCACCCTCCGGTGCAAATGGGATGGTAAGGAGTTTATTGTGAAAAAGTGGTTATTAGCTGCAGGTCTCGGTTTAGCACTGGCAACTTCTGCTCAGGCGGCTGACAAAATTGCAATCGTCAACAT‑GGCAGCCTGTTCcagc                                                                                                                                 >  2:1266130/1‑150 (MQ=255)

              gCAAAGAACTGCACCCTCCGGTGCAAATGGGATGGTAAGGAGTTTATTGTGAAAAAGTGGTTATTAGCTGCAGGTCTCGGTTTAGCACTGGCAACTTCTGCTCAGGCGGCTGACAAAATTGCAATCGTCAACAT‑GGCAGCCTGTTCcagc                                                                                                                                 >  2:136232/1‑150 (MQ=255)

                aaaGAACTGCACCCTCCGGTGCAAATGGGATGGTAAGGAGTTTATTGTGAAAAAGTGGTTATTAGCTGCAGGTCTCGGTTTAGCACTGGCAACTTCTGCTCAGGCGGCTGACAAAATTGCAATCGTCAACAT‑GGCAGCCTGTTCcagcag                                                                                                                               >  2:2450169/1‑150 (MQ=255)

                  aGAACTGCACCCTCCGGTGCAAATGGGATGGTAAGGAGTTTATTGTGAAAAAGTGGTTATTAGCTGCAGGTCTCGGTTTAGCACTGGCAACTTCTGCTCAGGCGGCTGACAAAATTGCAATCGTCAACAT‑GGCAGCCTGTTCCAGCAGGt                                                                                                                             >  2:3847791/1‑150 (MQ=255)

                  aGAACTGCACCCTCCGGTGCAAATGGGATGGTAAGGAGTTTATTGTGAAAAAGTGGTTATTAGCTGCAGGTCTCGGTTTAGCACTGGCAACTTCTGCTCAGGCGGCTGACAAAATTGCAATCGTCAACAT‑GGCAGCCTGTTCCAGCAGGt                                                                                                                             >  2:2349505/1‑150 (MQ=255)

                    aaCTGCACCCTCCGGTGCAAATGGGATGGTAAGGAGTTTATTGTGAAAAAGTGGTTATTAGCTGCAGGTCTCGGTTTAGCACTGGCAACTTCTGCTCAGGCGGCTGACAAAATTGCAATCGTCAACAT‑GGCAGCCTGTTCCAGCAGGTAg                                                                                                                           >  1:4078923/1‑150 (MQ=255)

                    aaCTGCACCCTCCGGTGCAAATGGGATGGTAAGGAGTTTATTGTGAAAAAGTGGTTATTAGCTGCAGGTCTCGGTTTAGCACTGGCAACTTCTGCTCAGGCGGCTGACAAAATTGCAATCGTCAACAT‑GGCAGCCTGTTCCAGCAGGTAg                                                                                                                           <  2:1908788/150‑1 (MQ=255)

                     aCTGCACCCTCCGGTGCAAATGGGATGGTAAGGAGTTTATTGTGAAAAAGTGGTTATTAGCTGCAGGTCTCGGTTTAGCACTGGCAACTTCTGCTCAGGCGGCTGACAAAATTGCAATCGTCAACAT‑GGCAGCCTGTTCCAGCAGGTAgc                                                                                                                          >  1:1020503/1‑150 (MQ=255)

                     aCTGCACCCTCCGGTGCAAATGGGATGGTAAGGAGTTTATTGTGAAAAAGTGGTTATTAGCTGCAGGTCTCGGTTTAGCACTGGCAACTTCTGCTCAGGCGGCTGACAAAATTGCAATCGTCAACAT‑GGCAGCCTGTTCCAGCAGGTAgc                                                                                                                          <  1:2711568/150‑1 (MQ=255)

                        gCACCCTCCGGTGCAAATGGGATGGTAAGGAGTTTATTGTGAAAAAGTGGTTATTAGCTGCAGGTCTCGGTTTAGCACTGGCAACTTCTGCTCAGGCGGCTGACAAAATTGCAATCGTCAACAT‑GGCAGCCTGTTCCAGCAGGTAGCGCa                                                                                                                       >  2:2781587/1‑150 (MQ=255)

                           cccTCCGGTGCAAATGGGATGGTAAGGAGTTTATTGTGAAAAAGTGGTTATTAGCTGCAGGTCTCGGTTTAGCACTGGCAACTTCTGCTCAGGCGGCTGACAAAATTGCAATCGTCAACAT‑GGCAGCCTGTTCCAGCAGGTAGCGCAGaa                                                                                                                    <  1:3052982/150‑1 (MQ=255)

                             cTCCGGTGCAAATGGGATGGTAAGGAGTTTATTGTGAAAAAGTGGTTATTAGCTGCAGGTCTCGGTTTAGCACTGGCAACTTCTGCTCAGGCGGCTGACAAAATTGCAATCGTCAACAT‑GGCAGCCTGTTCCAGCAGGTAGCGCAGaaaa                                                                                                                  >  2:4007030/1‑150 (MQ=255)

                             cTCCGGTGCAAATGGGATGGTAAGGAGTTTATTGTGAAAAAGTGGTTATTAGCTGCAGGTCTCGGTTTAGCACTGGCAACTTCTGCTCAGGCGGCTGACAAAATTGCAATCGTCAACAT‑GGCAGCCTGTTCCAGCAGGTAGCGCAGaaaa                                                                                                                  >  1:23069/1‑150 (MQ=255)

                               ccGGTGCAAATGGGATGGTAAGGAGTTTATTGTGAAAAAGTGGTTATTAGCTGCAGGTCTCGGTTTAGCACTGGCAACTTCTGCTCAGGCGGCTGACAAAATTGCAATCGTCAACAT‑GGCAGCCTGTTCCAGCAGGTAGCGCAGAAAAcc                                                                                                                >  1:2533550/1‑150 (MQ=255)

                                   tGCAAATGGGATGGTAAGGAGTTTATTGTGAAAAAGTGGTTATTAGCTGCAGGTCTCGGTTTAGCACTGGCAACTTCTGCTCAGGCGGCTGACAAAATTGCAATCGTCAACAT‑GGCAGCCTGTTCCAGCAGGTAGCGCAGAAAACCGgtg                                                                                                            <  1:3700606/150‑1 (MQ=255)

                                   tGCAAATGGGATGGTAAGGAGTTTATTGTGAAAAAGTGGTTATTAGCTGCAGGTCTCGGTTTAGCACTGGCAACTTCTGCTCAGGCGGCCGACAAAATTGCAATCGTCAACAT‑GGCAGCCTGTTCCAGCAGGTAGCGCAGAAAACCGgtg                                                                                                            <  1:1599328/150‑1 (MQ=255)

                                     cAAATGGGATGGTAAGGAGTTTATTGTGAAAAAGTGGTTATTAGCTGCAGGTCTCGGTTTAGCACTGGCAACTTCTGCTCAGGCGGCTGACAAAATTGCAATCGTCAACAT‑GGCAGCCTGTTCCAGCAGGTAGCGCAGAAAACCGGTGtt                                                                                                          >  2:532815/1‑150 (MQ=255)

                                     cAAATGGGATGGTAAGGAGTTTATTGTGAAAAAGTGGTTATTAGCTGCAGGTCTCGGTTTAGCACTGGCAACTTCTGCTCAGGCGGCTGACAAAATTGCAATCGTCAACAT‑GGCAGCCTGTTCCAGCAGGTAGCGCAGAAAACCGGTGtt                                                                                                          >  2:3904629/1‑150 (MQ=255)

                                     cAAATGGGATGGTAAGGAGTTTATTGTGAAAAAGTGGTTATTAGCTGCAGGTCTCGGTTTAGCACTGGCAACTTCTGCTCAGGCGGCTGACAAAATTGCAATCGTCAACAT‑GGCAGCCTGTTCCAGCAGGTAGCGCAGAAAACCGGTGtt                                                                                                          <  1:3507040/150‑1 (MQ=255)

                                      aaaTGGGATGGTAAGGAGTTTATTGTGAAAAAGTGGTTATTAGCTGCAGGTCTCGGTTTAGCACTGGCAACTTCTGCTCAGGCGGCTGACAAAATTGCAATCGTCAACAT‑GGCAGCCTGTTCCAGCAGGTAGCGCAGAAAACCGGTGttt                                                                                                         <  2:1507687/150‑1 (MQ=255)

                                       aaTGGGATGGTAAGGAGTTTATTGTGAAAAAGTGGTTATTAGCTGCAGGTCTCGGTTTAGCACTGGCAACTTCTGCTCAGGCGGCTGACAAAATTGCAATCGTCAACAT‑GGCAGCCTGTTCCAGCAGGTAGCGCAGAAAACCGGTGTTTc                                                                                                        >  2:1710002/1‑150 (MQ=255)

                                        aTGGGATGGTAAGGAGTTTATTGTGAAAAAGTGGTTATTAGCTGCAGGTCTCGGTTTAGCACTGGCAACTTCTGCTCAGGCGGCTGACAAAATTGCAATCGTCAACAT‑GGCAGCCTGTTCCAGCAGGTAGCGCAGAAAACCGGTGTTTCt                                                                                                       >  2:597995/1‑150 (MQ=255)

                                          gggATGGTAAGGAGTTTATTGTGAAAAAGTGGTTATTAGCTGCAGGTCTCGGTTTAGCACTGGCAACTTCTGCTCAGGCGGCTGACAAAATTGCAATCGTCAACAT‑GGCAGCCTGTTCCAGCAGGTAGCGCAGAAAACCGGTGTTTCTaa                                                                                                     >  1:2035566/1‑150 (MQ=255)

                                             aTGGTAAGGAGTTTATTGTGAAAAAGTGGTTATTAGCTGCAGGTCTCGGTTTAGCACTGGCAACTTCTGCTCAGGCGGCTGACAAAATTGCAATCGTCAACAT‑GGCAGCCTGTTCCAGCAGGTAGCGCAGAAAACCGGTGTTTCTAacac                                                                                                  >  2:1160260/1‑150 (MQ=255)

                                             aTGGTAAGGAGTTTATTGTGAAAAAGTGGTTATTAGCTGCAGGTCTCGGTTTAGCACTGGCAACTTCTGCTCAGGCGGCTGACAAAATTGCAATCGTCAACAT‑GGCAGCCTGTTCCAGCAGGTAGCGCAGAAAACCGGTGTTTCTAacac                                                                                                  >  2:3697643/1‑150 (MQ=255)

                                             aTGGTAAGGAGTTTATTGTGAAAAAGTGGTTATTAGCTGCAGGTCTCGGTTTAGCACTGGCAACTTCTGCTCAGGCGGCTGACAAAATTGCAATCGTCAACAT‑GGCAGCCTGTTCCAGCAGGTAGCGCAGAAAACCGGTGTTTATAacac                                                                                                  <  1:2239607/150‑1 (MQ=255)

                                              tGGTAAGGAGTTTATTGTGAAAAAGTGGTTATTAGCTGCAGGTCTCGGTTTAGCACTGGCAACTTCTGCTCAGGCGGCTGACAAAATTGCAATCGTCAACAT‑GGCAGCCTGTTCCAGCAGGTAGCGCAGAAAACCGGTGTTTCTAACACg                                                                                                 <  2:1297147/150‑1 (MQ=255)

                                                 tAAGGAGTTTATTGTGAAAAAGTGGTTATTAGCTGCAGGTCTCGGTTTAGCACTGGCAACTTCTGCTCAGGCGGCTGACAAAATTGCAATCGTCAACAT‑GGCAGCCTGTTCCAGCAGGTAGCGCAGAAAACCGGTGTTTCTAACACGCTg                                                                                              <  2:823159/150‑1 (MQ=255)

                                                  aaGGAGTTTATTGTGAAAAAGTGGTTATTAGCTGCAGGTCTCGGTTTAGCACTGGCAACTTCTGCTCAGGCGGCTGACAAAATTGCAATCGTCAACAT‑GGCAGCCTGTTCCAGCAGGTAGCGCAGAAAACCGGTGTTTCTAACACGCTgg                                                                                             >  2:332877/1‑150 (MQ=255)

                                                    ggAGTTTATTGTGAAAAAGTGGTTATTAGCTGCAGGTCTCGGTTTAGCACTGGCAACTTCTGCTCAGGCGGCTGACAAAATTGCAATCGTCAACAT‑GGCAGCCTGTTCCAGCAGGTAGCGCAGAAAACCGGTGTTTCTAACACGCTGGaa                                                                                           >  1:3316688/1‑150 (MQ=255)

                                                         ttATTGTGAAAAAGTGGTTATTAGCTGCAGGTCTCGGTTTAGCACTGGCAACTTCTGCTCAGGCGGCTGACAAAATTGCAATCGTCAACAT‑GGCAGCCTGTTCCAGCAGGTAGCGCAGAAAACCGGTGTTTCTAACACGCTGGAAAATGa                                                                                      >  2:2071254/1‑150 (MQ=255)

                                                           aTTGTGAAAAAGTGGTTATTAGCTGCAGGTCTCGGTTTAGCACTGGCAACTTCTGCTCAGGCGGCTGACAAAATTGCAATCGTCAACAT‑GGCAGCCTGTTCCAGCAGGTAGCGCAGAAAACCGGTGTTTCTAACACGCTGGAAAATGAGt                                                                                    >  2:3046410/1‑150 (MQ=255)

                                                           aTTGTGAAAAAGTGGTTATTAGCTGCAGGTCTCGGTTTAGCACTGGCAACTTCTGCTCAGGCGGCTGACAAAATTGCAATCGTCAACAT‑GGCAGCCTGTTCCAGCAGGTAGCGCAGAAAACCGGTGTTTCTAACACGCTGGAAAATGAGt                                                                                    >  2:1805548/1‑150 (MQ=255)

                                                            ttGTGAAAAAGTGGTTATTAGCTGCAGGTCTCGGTTTAGCACTGGCAACTTCTGCTCAGGCGGCTGACAAAATTGCAATCGTCAACAT‑GGCAGCCTGTTCCAGCAGGTAGCGCAGAAAACCGGTGTTTCTAACACGCTGGAAAATGAGtt                                                                                   >  1:1065557/1‑150 (MQ=255)

                                                                gAAAAAGTGGTTATTAGCTGCAGGTCTCGGTTTAGCACTGGCAACTTCTGCTCAGGCGGCTGACAAAATTGCAATCGTCAACAT‑GGCAGCCTGTTCCAGCAGGTAGCGCAGAAAACCGGTGTTTCTAACACGCTGGAAAATGAGTTCaaa                                                                               >  2:188859/1‑150 (MQ=255)

                                                                   aaaGTGGTTATTAGCTGCAGGTCTCGGTTTAGCACTGGCAACTTCTGCTCAGGCGGCTGACAAAATTGCAATCGTCAACAT‑GGCAGCCTGTTCCAGCAGGTAGCGCAGAAAACCGGTGTTTCTAACACGCTGGAAAATGAGTTCAAAGGc                                                                            >  1:1108104/1‑150 (MQ=255)

                                                                    aaGTGGTTATTAGCTGCAGGTCTCGGTTTAGCACTGGCAACTTCTGCTCAGGCGGCTGACAAAATTGCAATCGTCAACAT‑GGCAGCCTGTTCCAGCAGGTAGCGCAGAAAACCGGTGTTTCTAACACGCTGGAAAATGAGTTCAAAGGcc                                                                           <  1:2858663/150‑1 (MQ=255)

                                                                      gTGGTTATTAGCTGCAGGTCTCGGTTTAGCACTGGCAACTTCTGCTCAGGCGGCTGACAAAATTGCAATCGTCAACAT‑GGCAGCCTGTTCCAGCAGGTAGCGCAGAAAACCGGTGTTTCTAACACGCTGGAAAATGAGTTCAAAGGCCGt                                                                         >  2:1565996/1‑150 (MQ=255)

                                                                       tGGTTATTAGCTGCAGGTCTCGGTTTAGCACTGGCAACTTCTGCTCAGGCGGCTGACAAAATTGCAATCGTCAACAT‑GGCAGCCTGTTCCAGCAGGTAGCGCAGAAAACCGGTGTTTCTAACACGCTGGAAAATGAGTTCAAAGGCCGTg                                                                        >  1:1831598/1‑150 (MQ=255)

                                                                       tGGTTATTAGCTGCAGGTCTCGGTTTAGCACTGGCAACTTCTGCTCAGGCGGCTGACAAAATTGCAATCGTCAACAT‑GGCAGCCTGTTCCAGCAGGTAGCGCAGAAAACCGGTGTTTCTAACACGCTGGAAAATGAGTTCAAAGGCCGTg                                                                        >  2:2229488/1‑150 (MQ=255)

                                                                       tGGTTATTAGCTGCAGGTCTCGGTTTAGCACTGGCAACTTCTGCTCAGGCGGCTGACAAAATTGCAATCGTCAACAT‑GGCAGCCTGTTCCAGCAGGTAGCGCAGAAAACCGGTGTTTCTAACACGCTGGAAAATGAGTTCAAAGGCCGTg                                                                        <  2:2418709/150‑1 (MQ=255)

                                                                          ttattaGCTGCAGGTCTCGGTTTAGCACTGGCAACTTCTGCTCAGGCGGCTGACAAAATTGCAATCGTCAACAT‑GGCAGCCTGTTCCAGCAGGTAGCGCAGAAAACCGGTGTTTCTAACACGCTGGAAAATGAGTTCAAAGGCCGTGCCa                                                                     <  2:3754177/150‑1 (MQ=255)

                                                                           tattaGCTGCAGGTCTCGGTTTAGCACTGGCAACTTCTGCTCAGGCGGCTGACAAAATTGCAATCGTCAACAT‑GGCAGCCTGTTCCAGCAGGTAGCGCAGAAAACCGGTGTTTCTAACACGCTGGAAAATGAGTTCAAAGGCCGTGCCAg                                                                    >  2:201257/1‑150 (MQ=255)

                                                                           tattaGCTGCAGGTCTCGGTTTAGCACTGGCAACTTCTGCTCAGGCGGCTGACAAAATTGCAATCGTCAACAT‑GGCAGCCTGTTCCAGCAGGTAGCGCAGAAAACCGGTGTTTCTAACACGCTGGAAAATGAGTTCAAAGGCCGTGCCAg                                                                    >  1:1569471/1‑150 (MQ=255)

                                                                            attaGCTGCAGGTCTCGGTTTAGCACTGGCAACTTCTGCTCAGGCGGCTGACAAAATTGCAATCGTCAACAT‑GGCAGCCTGTTCCAGCAGGTAGCGCAGAAAACCGGTGTTTCTAACACGCTGGAAAATGAGTTCAAAGGCCGTGCCAGc                                                                   <  1:3180039/150‑1 (MQ=255)

                                                                              taGCTGCAGGTCTCGGTTTAGCACTGGCAACTTCTGCTCAGGCGGCTGACAAAATTGCAATCGTCAACAT‑GGCAGCCTGTTCCAGCAGGTAGCGCAGAAAACCGGTGTTTCTAACACGCTGGAAAATGAGTTCAAAGGCCGTGCCAGCGa                                                                 <  1:1733900/150‑1 (MQ=255)

                                                                               aGCTGCAGGTCTCGGTTTAGCACTGGCAACTTCTGCTCAGGCGGCTGACAAAATTGCAATCGTCAACAT‑GGCAGCCTGTTCCAGCAGGTAGCGCAGAAAACCGGTGTTTCTAACACGCTGGAAAATGAGTTCAAAGGCCGTGCCAGCGaa                                                                >  1:128255/1‑150 (MQ=255)

                                                                                   gCAGGTCTCGGTTTAGCACTGGCAACTTCTGCTCAGGCGGCTGACAAAATTGCAATCGTCAACAT‑GGCAGCCTGTTCCAGCAGGTAGCGCAGAAAACCGGTGTTTCTAACACGCTGGAAAATGAGTTCAAAGGCCGTGCCAGCGAACTGc                                                            >  1:2099491/1‑150 (MQ=255)

                                                                                      ggTCTCGGTTTAGCACTGGCAACTTCTGCTCAGGCGGCTGACAAAATTGCAATCGTCAACAT‑GGCAGCCTGTTCCAGCAGGTAGCGCAGAAAACCGGTGTTTCTAACACGCTGGAAAATGAGTTCAAAGGCCGTGCCAGCGAACTGCAGc                                                         <  2:1055546/150‑1 (MQ=255)

                                                                                      ggTCTCGGTTTAGCACTGGCAACTTCTGCTCAGGCGGCTGACAAAATTGCAATCGTCAACAT‑‑GCAGCCTGTTCCAGCAGGTAGCGCAGAAAACCTGTGTTTCTAACACGCTGGAAAATGAGTTCAAAGGCCGTGCCAGCGAACTGCAGCg                                                        <  2:3258145/150‑1 (MQ=255)

                                                                                       gTCTCGGTTTAGCACTGGCAACTTCTGCTCAGGCGGCTGACAAAATTGCAATCGTCAACAT‑GGCAGCCTGTTCCAGCAGGTAGCGCAGAAAACCGGTGTTTCTAACACGCTGGAAAATGAGTTCAAAGGCCGTGCCAGCGAACTGCAGCg                                                        >  2:1026076/1‑150 (MQ=255)

                                                                                          tcGGTTTAGCACTGGCAACTTCTGCTCAGGCGGCTGACAAAATTGCAATCGTCAACAT‑GGCAGCCTGTTCCAGCAGGTAGCGCAGAAAACCGGTGTTTCTAACACGCTGGAAAATGAGTTCAAAGGCCGTGCCAGCGAACTGCAGCGTAt                                                     <  1:867876/150‑1 (MQ=255)

                                                                                            ggTTTAGCACTGGCAACTTCTGCTCAGGCGGCTGACAAAATTGCAATCGTCAACAT‑GGCAGCCTGTTCCAGCAGGTAGCGCAGAAAACCGGTGTTTCTAACACGCTGGAAAATGAGTTCAAAGGCCGTGCCAGCGAACTGCAGCGTATgg                                                   >  2:4130759/1‑150 (MQ=255)

                                                                                              tttAGCACTGGCAACTTCTGCTCAGGCGGCTGACAAAATTGCAATCGTCAACAT‑GGCAGCCTGTTCCAGCAGGTAGCGCAGAAAACCGGTGTTTCTAACACGCTGGAAAATGAGTTCAAAGGCCGTGCCAGCGAACTGCAGCGTATGGaa                                                 >  2:2534982/1‑150 (MQ=255)

                                                                                              tttAGCACTGGCAACTTCTGCTCAGGCGGCTGACAAAATTGCAATCGTCAACAT‑GGCAGCCTGTTCCAGCAGGTAGCGCAGAAAACCGGTGTTTCTAACACGCTGGAAAATGAGTTCAAAGGCCGTGCCAGCGAACTGCAGCGTATGGaa                                                 >  1:895916/1‑150 (MQ=255)

                                                                                              tttAGCACTGGCAACTTCTGCTCAGGCGGCTGACAAAATTGCAATCGTCAACAT‑GGCAGCCTGTTCCAGCAGGTAGCGCAGAAAACCGGTGTTTCTAACACGCTGGAAAATGAGTTCAAAGGCCGTGCCAGCGAACTGCAGCGTATGGaa                                                 <  2:3649955/150‑1 (MQ=255)

                                                                                                tAGCACTGGCAACTTCTGCTCAGGCGGCTGACAAAATTGCAATCGTCAACAT‑GGCAGCCTGTTCCAGCAGGTAGCGCAGAAAACCGGTGTTTCTAACACGCTGGAAAATGAGTTCAAAGGCCGTGCCAGCGAACTGCAGCGTATGGAAAc                                               >  1:1884165/1‑150 (MQ=255)

                                                                                                tAGCACTGGCAACTTCTGCTCAGGCGGCTGACAAAATTGCAATCGTCAACAT‑GGCAGCCTGTTCCAGCAGGTAGCGCAGAAAACCGGTGTTTCTAACACGCTGGAAAATGAGTTCAAAGGCCGTGCCAGCGAACTGCAGCGTATGGAAAc                                               >  1:755492/1‑150 (MQ=255)

                                                                                                  gCACTGGCAACTTCTGCTCAGGCGGCTGACAAAATTGCAATCGTCAACAT‑GGCAGCCTGTTCCAGCAGGTAGCGCAGAAAACCGGTGTTTCTAACACGCTGGAAAATGAGTTCAAAGGCCGTGCCAGCGAACTGCAGCGTATGGAAACCg                                             <  1:2978770/150‑1 (MQ=255)

                                                                                                    aCTGGCAACTTCTGCTCAGGCGGCTGACAAAATTGCAATCGTCAACAT‑GGCAGCCTGTTCCAGCAGGTAGCGCAGAAAACCGGTGTTTCTAACACGCTGGAAAATGAGTTCAAAGGCCGTGCCAGCGAACTGCAGCGTATGGAAACCGAt                                           <  2:497725/150‑1 (MQ=255)

                                                                                                     cTGGCAACTTGTGCTCAGGCGGCTGACAAAATTGCAATCGTCAACAT‑GGCAGCCTGTTCCAGCAGGTAGCGCAGAAAACCGGTGTTTCTAACACGCTGGAAAATGAGTTCAAAGGCCGTGCCAGCGAACTGCAGCGTATGGAAACCGATc                                          <  1:4130018/150‑1 (MQ=255)

                                                                                                      tGGCAACTTCTGCTCAGGCGGCTGACAAAATTGCAATCGTCAACAT‑GGCAGCCTGTTCCAGCAGGTAGCGCAGAAAACCGGTGTTTCTAACACGCTGGAAAATGAGTTCAAAGGCCGTGCCAGCGAACTGCAGCGTATGGAAACCGATCt                                         >  2:2166959/1‑150 (MQ=255)

                                                                                                      tGGCAACTTCTGCTCAGGCGGCTGACAAAATTGCAATCGTCAACAT‑GGCAGCCTGTTCCAGCAGGTAGCGCAGAAAACCGGTGTTTCTAACACGCTGGAAAATGAGTTCAAAGGCCGTGCCAGCGAACTGCAGCGTATGGAAACCGATCt                                         <  2:2190293/150‑1 (MQ=255)

                                                                                                       ggCAACTTCTGCTCAGGCGGCTGACAAAATTGCAATCGTCAACAT‑GGCAGCCTGTTCCAGCAGGTAGCGCAGAAAACCGGTGTTTCTAACACGCTGGAAAATGAGTTCAAAGGCCGTGCCAGCGAACTGCAGCGTATGGAAACCGATCTg                                        <  1:279587/150‑1 (MQ=255)

                                                                                                       ggCAACTTCTGCTCAGGCGGCTGACAAAATTGCAATCGTCAACAT‑GGCAGCCTGTTCCAGCAGGTAGCGCAGAAAACCGGTGTTTCTAACACGCTGGAAAATGAGTTCAAAGGCCGTGCCAGCGAACTGCAGCGTATGGAAACCGATCTg                                        <  1:322296/150‑1 (MQ=255)

                                                                                                        gCAACTTCTGCTCAGGCGGCTGACAAAATTGCAATCGTCAACAT‑GGCAGCCTGTTCCAGCAGGTAGCGCAGAAAACCGGTGTTTCTAACACGCTGGAAAATGAGTTCAAAGGCCGTGCCAGCGAACTGCAGCGTATGGAAACCGATCTGc                                       >  2:2930287/1‑150 (MQ=255)

                                                                                                         cAACTTCTGCTCAGGCGGCTGACAAAATTGCAATCGTCAACAT‑GGCAGCCTGTTCCAGCAGGTAGCGCAGAAAACCGGTGTTTCTAACACGCTGGAAAATGAGTTCAAAGGCCGTGCCAGCGAACTGCAGCGTATGGAAACCGATCTGCa                                      <  2:2675402/150‑1 (MQ=255)

                                                                                                               cTGCTCAGGCGGCTGACAAAATTGCAATCGTCAACAT‑GGCAGCCTGTTCCAGCAGGTAGCGCAGAAAACCGGTGTTTCTAACACGCTGGAAAATGAGTTCAAAGGCCGTGCCAGCGAACTGCAGCGTATGGAAACCGATCTGCAGGCTaa                                >  2:1173516/1‑150 (MQ=255)

                                                                                                                tGCTCAGGCGGCTGACAAAATTGCAATCGTCAACAT‑GGCAGCCTGTTCCAGCAGGTAGCGCAGAAAACCGGTGTTTCTAACACGCTGGAAAATGAGTTCAAAGGCCGTGCCAGCGAACTGCAGCGTATGGAAACCGATCTGCAGGCTaaa                               >  1:4007003/1‑150 (MQ=255)

                                                                                                                 gCTCAGGCGGCTGACAAAATTGCAATCGTCAACAT‑GGCAGCCTGTTCCAGCAGGTAGCGCAGAAAACCGGTGTTTCTAACACGCTGGAAAATGAGTTCAAAGGCCGTGCCAGCGAACTGCAGCGTATGGAAACCGATCTGCAGGCTaaaa                              >  2:2831871/1‑150 (MQ=255)

                                                                                                                   tCAGGCGGCTGACAAAATTGCAATCGTCAACAT‑GGCAGCCTGTTCCAGCAGGTAGCGCAGAAAACCGGTGTTTCTAACACGCTGGAAAATGAGTTCAAAGGCCGTGCCAGCGAACTGCAGCGTATGGAAACCGATCTGCAGGCTAAAATg                            <  1:1702719/150‑1 (MQ=255)

                                                                                                                   tCAGGCGGCTGACAAAATTGCAATCGTCAACAT‑GGCAGCCTGTTCCAGCAGGTAGCGCAGAAAACCGGTGTTTCTAACACGCTGGAAAATGAGTTCAAAGGCCGTGCCAGCGAACTGCAGCGTATGGAAACCGATCTGCAGGCTAAAATg                            <  1:2351889/150‑1 (MQ=255)

                                                                                                                        cggcTGACAAAATTGCAATCGTCAACAT‑GGCAGCCTGTTCCAGCAGGTAGCGCAGAAAACCGGTGTTTCTAACACGCTGGAAAATGAGTTCAAAGGCCGTGCCAGCGAACTGCAGCGTATGGAAACCGATCTGCAGGCTAAAATGaaaaa                       >  2:2040327/1‑150 (MQ=255)

                                                                                                                         ggcTGACAAGATTGCAATCGTCAACAT‑GGCAGCCTGTTCCAGCAGGTAGCGCAGAAAACCGGTGTTTCTAACACGCTGGAAAATGAGTTCAAAGGCCGTGCCAGCGAACTGCAGCGTATGGAAACCGATCTGCATGCTAAAATGAAAAAg                      >  2:3428981/1‑150 (MQ=255)

                                                                                                                             gACAAAATTGCAATCGTCAACAT‑GGCAGCCTGTTCCAGCAGGTAGCGCAGAAAACCGGTGTTTCTAACACGCTGGAAAATGAGTTCAAAGGCCGTGCCAGCGAACTGCAGCGTATGGAAACCGATCTGCAGGCTAAAATGAAAAAGCTGc                  <  1:1483732/150‑1 (MQ=255)

                                                                                                                             gACAAAATTGCAATCGTCAACAT‑GGCAGCCTGTTCCAGCAGGTAGCGCAGAAAACCGGTGTTTCTAACACGCTGGAAAATGAGTTCAAAGGCCGTGCCAGCGAACTGCAGCGTATGGAAACCGATCTGCAGGCTAAAATGAAAAAGCTGc                  <  1:3869530/150‑1 (MQ=255)

                                                                                                                               cAAAATTGCAATCGTCAACAT‑GGCAGCCTGTTCCAGCAGGTAGCGCAGAAAACCGGTGTTTCTAACACGCTGGAAAATGAGTTCAAAGGCCGTGCCAGCGAACTGCAGCGTATGGAAACCGATCTGCAGGCTAAAATGAAAAAGCTGCAg                >  2:2956923/1‑150 (MQ=255)

                                                                                                                               cAAAATTGCAATCGTCAACAT‑GGCAGCCTGTTCCAGCAGGTAGCGCAGAAAACCGGTGTTTCTAACACGCTGGAAAATGAGTTCAAAGGCCGTGCCAGCGAACTGCAGCGTATGGAAACCGATCTGCAGGCTAAAATGAAAAAGCTGCAg                <  1:343408/150‑1 (MQ=255)

                                                                                                                                aaaaTTGCAATCGTCAACAT‑GGCAGCCTGTTCCAGCAGGTAGCGCAGAAAACCGGTGTTTCTAACACGCTGGAAAATGAGTTCAAAGGCCGTGCCAGCGAACTGCAGCGTATGGAAACCGATCTGCAGGCTAAAATGAAAAAGCTGCAGt               >  1:983717/1‑150 (MQ=255)

                                                                                                                                aaaaTTGCAATCGTCAACAT‑GGCAGCCTGTTCCAGCAGGTAGCGCAGAAAACCGGTGTTTCTAACACGCTGGAAAATGAGTTCAAAGGCCGTGCCAGCGAACTGCAGCGTATGGAAACCGATCTGCAGGCTAAAATGAAAAAGCTGCAGt               <  1:2603807/150‑1 (MQ=255)

                                                                                                                                   aTTGCAATCGTCAACAT‑GGCAGCCTGTTCCAGCAGGTAGCGCAGAAAACCGGTGTTTCTAACACGCTGGAAAATGAGTTCAAAGGCCGTGCCAGCGAACTGCAGCGTATGGAAACCGATCTGCAGGCTAAAATGAAAAAGCTGCAGTCCa            <  1:1166929/150‑1 (MQ=255)

                                                                                                                                    ttGCAATCGTCAACAT‑GGCAGCCTGTTCCAGCAGGTAGCGCAGAAAACCGGTGTTTCTAACACGCTGGAAAATGAGTTCAAAGGCCGTGCCAGCGAACTGCAGCGTATGGAAACCGATCTGCAGGCTAAAATGAAAAAGCTGCAGTCCAt           >  2:3079001/1‑150 (MQ=255)

                                                                                                                                      gCAATCGTCAACAT‑GGCAGCCTGTTCCAGCAGGTAGCGCAGAAAACCGGTGTTTCTAACACGCTGGAAAATGAGTTCAAAGGCCGTGCCAGCGAACTGCAGCGTATGGAAACCGATCTGCAGGCTAAAATGAAAAAGCTGCAGTCCATGa         <  1:621535/150‑1 (MQ=255)

                                                                                                                                      gCAATCGTCAACAT‑GGCAGCCTGTTCCAGCAGGTAGCGCAGAAAACCGGTGTTTCTAACACGCTGGAAAATGAGTTCAAAGGCCGTGCCAGCGAACTGCAGCGTATGGAAACCGATCTGCAGGCTAAAATGAAAAAGCTGCAGTCCATGa         <  1:3596659/150‑1 (MQ=255)

                                                                                                                                      gCAATCGTCAACAT‑GGCAGCCTGTTCCAGCAGGTAGCGCAGAAAACCGGTGTTTCTAACACGCTGGAAAATGAGTTCAAAGGCCGTGCCAGCGAACTGCAGCGTATGGAAACCGATCTGCAGGCTAAAATGAAAAAGCTGCAGTCCATGa         >  1:195445/1‑150 (MQ=255)

                                                                                                                                       cAATCGTCAACAT‑GGCAGCCTGTTCCAGCAGGTAGCGCAGAAAACCGGTGTTTCTAACACGCTGGAAAATGAGTTCAAAGGCCGTGCCAGCGAACTGCAGCGTATGGAAACCGATCTGCAGGCTAAAATGAAAAAGCTGCAGTCCATGaa        <  1:3977158/150‑1 (MQ=255)

                                                                                                                                       cAATCGTCAACAT‑GGCAGCCTGTTCCAGCAGGTAGCGCAGAAAACCGGTGTTTCTAACACGCTGGAAAATGAGTTCAAAGGCCGTGCCAGCGAACTGCAGCGTATGGAAACCGATCTGCAGGCTAAAATGAAAAAGCTGCAGTCCATGaa        >  1:3379484/1‑150 (MQ=255)

                                                                                                                                        aaTCGTCAACAT‑GGCAGCCTGTTCCAGCAGGTAGCGCAGAAAACCGGTGTTTCTAACACGCTGGAAAATGAGTTCAAAGGCCGTGCCAGCGAACTGCAGCGTATGGAAACCGATCTGCAGGCTAAAATGAAAAAGCTGCAGTCCATGaaa       <  1:1588417/150‑1 (MQ=255)

                                                                                                                                        aaTCGTCAACAT‑GGCAGCCTGTTCCAGCAGGTAGCGCAGAAAACCGGTGTTTCTAACACGCTGGAAAATGAGTTCAAAGGCCGTGCCAGCGAACTGCAGCGTATGGAAACCGATCTGCAGGCTAAAATGAAAAAGCTGCAGTCCATGaaa       <  2:894543/150‑1 (MQ=255)

                                                                                                                                         aTCGTCAACAT‑GGCAGCCTGTTCCAGCAGGTAGCGCAGAAAACCGGTGTTTCTAACACGCTGGAAAATGAGTTCAAAGGCCGTGCCAGCGAACTGCAGCGTATGGAAACCGATCTGCAGGCTAAAATGAAAAAGCTGCAGTCCATGAAAg      <  2:1639288/150‑1 (MQ=255)

                                                                                                                                           cGTCAACAT‑GGCAGCCTGTTCCAGCAGGTAGCGCAGAAAACCGGTGTTTCTAACACGCTGGAAAATGAGTTCAAAGGCCGTGCCAGCGAACTGCAGCGTATGGAAACCGATCTGCAGGCTAAAATGAAAAAGCTGCAGTCCATGAAAGCg    <  1:1389236/150‑1 (MQ=255)

                                                                                                                                            gTCAACAT‑GGCAGCCTGTTCCAGCAGGTAGCGCAGAAAACCGGTGTTTCTAACACGCTGGAAAATGAGTTCAAAGGCCGTGCCAGCGAACTGCAGCGTATGGAAACCGATCTGCAGGCTAAAATGAAAAAGCTGCAGTCCATGAAAGCgg   <  1:542599/150‑1 (MQ=255)

GATCGCCGGGCCACGCAAAGAACTGCACCCTCCGGTGCAAATGGGATGGTAAGGAGTTTATTGTGAAAAAGTGGTTATTAGCTGCAGGTCTCGGTTTAGCACTGGCAACTTCTGCTCAGGCGGCTGACAAAATTGCAATCGTCAACATGGGCAGCCTGTTCCAGCAGGTAGCGCAGAAAACCGGTGTTTCTAACACGCTGGAAAATGAGTTCAAAGGCCGTGCCAGCGAACTGCAGCGTATGGAAACCGATCTGCAGGCTAAAATGAAAAAGCTGCAGTCCATGAAAGCGGG  >  AP012306/189281‑189572

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`