Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP012306800,6750AC26.8% 671.2 / 31.9 280T192P (ACC→CCC) lolAchaperone for lipoproteins
Reads supporting (aligned to +/- strand):  ref base A (156/49);  new base C (0/75);  total (156/124)
Fisher's exact test for biased strand distribution p-value = 4.01e-35
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 328 total aligned reads displayed.

GCAATGGCAATCTGAAGCAGTTCACCATTAACGTGGGACGTGATGGCACAATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTG‑AAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTT‑CACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCAGAAAATTTAGCACAGTATATCGGCCAGCAACA  >  AP012306/800526‑800824
                                                                                                                                                       |                                                                                                                                                     
  aaTGGCAATCTGAAGCAGTTCACCATTAACGTGGGACGTGATGGCACAATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTG‑AAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTT‑CAcc                                                                                                                                                     >  1:575931/1‑150 (MQ=255)
  aaTGGCAATCTGAAGCAGTTCACCATTAACGTGGGACGTGATGGCACAATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTG‑AAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTT‑CAcc                                                                                                                                                     >  2:3607906/1‑150 (MQ=255)
  aaTGGCAATCTGAAGCAGTTCACCATTAACGTGGGACGTGATGGCACAATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTG‑AAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTT‑CAcc                                                                                                                                                     >  2:2955373/1‑150 (MQ=255)
   aTGGCAATCTGAAGCAGTTCACCATTAACGTGGGACGTGATGGCACAATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTG‑AAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTT‑CAccc                                                                                                                                                    >  2:3299677/1‑150 (MQ=255)
    tGGCAATCTGAAGCAGTTCACCATTAACGTGGGACGTGATGGCACAATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTG‑AAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTT‑CAcccc                                                                                                                                                   <  2:2557132/150‑1 (MQ=255)
       cAATCTGAAGCAGTTCACCATTAACGTGGGACGTGATGGCACAATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTG‑AAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTT‑CACCccgcc                                                                                                                                                >  2:2597470/1‑150 (MQ=255)
           cTGAAGCAGTTCACCATTAACGTGGGACGTGATGGCACAATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTG‑AAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTT‑CACCCCGCCGCaa                                                                                                                                            >  2:822820/1‑150 (MQ=255)
            tGAAGCAGTTCACCATTAACGTGGGACGTGATGGCACAATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTG‑AAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTT‑CACCCCGCCGCAAg                                                                                                                                           >  2:1413916/1‑150 (MQ=255)
              aaGCAGTTCACCATTAACGTGGGACGTGATGGCACAATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTG‑AAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTT‑CACCCCGCCGCAAGGc                                                                                                                                         >  2:3532199/1‑150 (MQ=255)
               aGCAGTTCACCATTAACGTGGGACGTGATGGCACAATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTG‑AAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTT‑CACCCCGCCGCAAGGCg                                                                                                                                        >  2:807641/1‑150 (MQ=255)
                  aGTTCACCATTAACGTGGGACGTGATGGCACAATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTG‑AAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTT‑CACCCCGCCGCAAGGCGTCa                                                                                                                                     >  1:461849/1‑150 (MQ=255)
                  aGTTCACCATTAACGTGGGACGTGATGGCACAATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTG‑AAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTT‑CACCCCGCCGCAAGGCGTCa                                                                                                                                     >  1:3852617/1‑150 (MQ=255)
                  aGTTCACCATTAACGTGGGACGTGATGGCACAATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTG‑AAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTT‑CACCCCGCCGCAAGGCGTCa                                                                                                                                     >  2:2255000/1‑150 (MQ=255)
                         cATTAACGTGGGACGTGATGGCACAATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTG‑AAATCCCAGCAAAAGGGGGCTGTGGATGCAGCGAAATTTACCTT‑CCCCCCGCCGCAAGGCGTCACGGTAga                                                                                                                              <  1:1026797/150‑1 (MQ=255)
                          aTTAACGTGGGACGTGATGGCACAATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTG‑AAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTT‑CACCCCGCCGCAAGGCGTCACGGTAgat                                                                                                                             >  1:2839524/1‑150 (MQ=255)
                          aTTAACGTGGGACGTGATGGCACAACCCATCAGTTTAGCGGGGTGGAGCAGAACGATCAGCCCAGCAGTTATCAACTG‑AATGCCCAGCAAAATGGGGCTGTGGAGGAAGCAAAATTTACCTC‑CCCCCCGCCGCAAGGCGTCACGGTAgat                                                                                                                             <  2:2082178/150‑1 (MQ=255)
                             aaCGTGGGACGTGATGGCACAATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTG‑AAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTT‑CACCCCGCCGCAAGGCGTCACGGTAgatgat                                                                                                                          >  2:2903149/1‑150 (MQ=255)
                               cGTGGGACGTGATGGCACAATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTG‑AAACCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTCCCTT‑CCCCCCGCCGCAAGGCGTCACGGTAGATGATCa                                                                                                                        <  2:665247/150‑1 (MQ=255)
                                 tGGGACGTGATGGCACAATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTG‑AAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTT‑CACCCCGCCGCAAGGCGTCACGGTAGATGATCAAc                                                                                                                      >  2:2768340/1‑150 (MQ=255)
                                   ggACGTGATGGCACAATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTG‑AAATCCCACCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTT‑CACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGt                                                                                                                    >  2:2669718/1‑150 (MQ=255)
                                      cGTGATGGCACAATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTG‑AAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTT‑CACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAg                                                                                                                 >  1:2801665/1‑150 (MQ=255)
                                       gTGATGGCACAATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTG‑AAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTT‑CACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGt                                                                                                                >  1:3590378/1‑150 (MQ=255)
                                        tGATGGCACAATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTG‑AAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTT‑CCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTa                                                                                                               <  1:2154237/150‑1 (MQ=255)
                                        tGATGGCACAATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTG‑AAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTT‑CACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTa                                                                                                               >  1:1828155/1‑150 (MQ=255)
                                         gATGGCACAATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTG‑AAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTT‑CACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTag                                                                                                              >  2:2831793/1‑150 (MQ=255)
                                           tGGCACAATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTG‑AAATCCCAGCAAAATGGGGCTGTGGATGCAGCAAAATTTCCCTT‑CCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTagag                                                                                                            <  1:1755936/150‑1 (MQ=255)
                                            ggCAGAATCCATCGTTTTAGCGGGGTGGAGCAGGACGATCAGCGCAGAAGTTATCAACTA‑AAACCCCAGAAAAATGGGCCTGTGGATTAAGCAAAATTTACCTT‑CCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAgg                                                                                                           <  2:493545/150‑1 (MQ=255)
                                             gCACAATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTG‑AAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTT‑CACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGc                                                                                                          >  2:1931521/1‑150 (MQ=255)
                                             gCAAAACCCACCAGTTTAGCGCGGTGGAGCAGGACGACCAGCGCAGCAGTTATCAACTA‑AACCCCAAGAAAAATGGGGTTGTGGATGCAGC‑AAATTTTCCTTCCCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGc                                                                                                          <  2:2983415/150‑1 (MQ=255)
                                               acaATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTG‑AAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTT‑CACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCAc                                                                                                        >  1:3438116/1‑150 (MQ=255)
                                                caATCCATCAGTTTAGCGCGGTGGAGCAGGACGATAAGCGCAGCAGTTATCAACTG‑AAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTCCCTC‑CCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCAcc                                                                                                       <  1:1349925/150‑1 (MQ=255)
                                                  acccAGCAGTTTAGCGCGGGGGAGCAGCACCACCAGCCGAGCAGTTATCACCTG‑AAAGCCCAACAAAATGGGGCTGTGGATGCTGCGAATTTTCCCTT‑CCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGGAGAGGCACCtg                                                                                                     <  2:3248166/148‑1 (MQ=255)
                                                    ccATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTG‑AAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTT‑CACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCtgag                                                                                                   >  1:1091723/1‑150 (MQ=255)
                                                    ccATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTG‑AAACCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTT‑CCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCtgag                                                                                                   <  2:3700028/150‑1 (MQ=255)
                                                      aTCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTG‑AAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTT‑CACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCtgagtg                                                                                                 >  2:2635256/1‑150 (MQ=255)
                                                        cAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTG‑AAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTT‑CACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCtgggtgat                                                                                               >  2:424350/1‑149 (MQ=255)
                                                           tttAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTG‑AAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTT‑CACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCaa                                                                                            >  2:3776550/1‑150 (MQ=255)
                                                           tttAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTG‑AAATCCCAGCAAAAGGGGGCTGTGGATGCAGCGAAATTTCCCTT‑CACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCaa                                                                                            <  2:3481590/150‑1 (MQ=255)
                                                            ttAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTG‑AAATCCCAGCAAAATGGGGCTGGGGATGCAGCGAAATTTACCTT‑CACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAAt                                                                                           <  2:1297684/150‑1 (MQ=255)
                                                             tAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTG‑AAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTT‑CACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATc                                                                                          <  1:2296/150‑1 (MQ=255)
                                                              aGCGCGGTGGGGCGGGACGATCAGCGCAGAATTTA‑CAACCGAAAACCCCAGAAAAGGGGGGCTGTGGAGGCAGCAAATTTTCCCTC‑CCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCt                                                                                         <  2:2023776/150‑1 (MQ=255)
                                                              aGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTG‑AAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTT‑CACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCt                                                                                         >  1:4189978/1‑150 (MQ=255)
                                                                cgcgGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTG‑AAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTT‑CACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGt                                                                                       <  1:1487951/150‑1 (MQ=255)
                                                                 gcgGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTG‑AAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTT‑CCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTc                                                                                      <  2:401432/150‑1 (MQ=255)
                                                                 gcgGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTA‑AAACCCCAGAAAAATGGGGCTGTGGATGCAGCAAAATTTACCTT‑CCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTc                                                                                      <  1:755273/150‑1 (MQ=255)
                                                                    gTGGAGCAGAAGGATCAGGGCAGCAGTTAGCAACTG‑AAATCCCACCAAAATGGGCCGGTGGATGCAGCGAAATTTCCCTT‑CTCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCt                                                                                   <  2:1503256/150‑1 (MQ=255)
                                                                      ggAGCAGGACGATGAGCGCAGCAGTTATCAACTG‑AAATCCGAGCAAAAGGGGGCGGTGGATGCAGCGAAATTTCCCTT‑CCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCg                                                                                 <  2:1612027/150‑1 (MQ=255)
                                                                        aGCAGGACGATCAGCGCAGCAGTTATCAACTG‑AAATCCCAGAAAAATGGGGTTGTGGATGCAGCGAAATTTACCTT‑CACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGAt                                                                               <  2:243478/150‑1 (MQ=255)
                                                                          cAGGACGATCAGCGCAGCAGTTATCAACTG‑AAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTT‑CACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGAttt                                                                             >  1:2413779/1‑150 (MQ=255)
                                                                            ggACGATCAGCGCAGCAGTTATCAACTA‑AAATCCCAGAAAAATGGGGCTGTGGATGCAGCGAAATTTACCTT‑CACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGAttttt                                                                           <  1:649596/150‑1 (MQ=255)
                                                                             gACGATCAGCGCAGCAGTTATCAACTG‑AAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTT‑CACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTc                                                                          >  1:2081286/1‑150 (MQ=255)
                                                                             gACGATCAGCGCAGCAGTTATCAACTG‑AAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTT‑CACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTc                                                                          >  2:371765/1‑150 (MQ=255)
                                                                              aCGATCAGCGCAGCAGTTATCAACTG‑AAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTT‑CACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCg                                                                         >  2:384072/1‑150 (MQ=255)
                                                                               cGATCAGCGCAGCAGTTATCAACTG‑AAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTT‑CACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCgg                                                                        >  1:847013/1‑150 (MQ=255)
                                                                                gATCAGCGCAGCAGTTATCAACTG‑AAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTT‑CACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGa                                                                       >  1:1449447/1‑150 (MQ=255)
                                                                                 aTCAGCGCAGCAGTTATCAACTG‑AAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTT‑CACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGat                                                                      >  2:2088615/1‑150 (MQ=255)
                                                                                  tCAGCGCAGCAGTTATCAACTG‑AAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTT‑CACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGata                                                                     <  1:2558783/150‑1 (MQ=255)
                                                                                    aGCGCAGCAGTTATCAACTG‑AAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTT‑CACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGataat                                                                   <  1:1903120/150‑1 (MQ=255)
                                                                                    aGCGCAGCAGTTATCAACTA‑AAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTC‑CCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGataat                                                                   <  2:450665/150‑1 (MQ=255)
                                                                                      cgcAGCAGTTATCAACTG‑AAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTT‑CACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATAc                                                                 >  2:13660/1‑150 (MQ=255)
                                                                                       gcagcaGTTATCAACTG‑AAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTT‑CCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACt                                                                <  1:3505571/150‑1 (MQ=255)
                                                                                       gcagcaGTTATCAACTG‑AAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTT‑CACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACt                                                                >  1:2961692/1‑150 (MQ=255)
                                                                                        cacctgTTATCAACTA‑AACCCCCAGCAAAGGGGGGCTGTGGATCCAGCAAAATTTCCCTT‑CCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACtt                                                               <  2:3129223/145‑1 (MQ=255)
                                                                                         agcagTTATCAACTG‑AAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTT‑CACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACttt                                                              >  2:4139509/1‑150 (MQ=255)
                                                                                           cagTTATCAACTG‑AAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTT‑CACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTc                                                            >  2:1704397/1‑150 (MQ=255)
                                                                                                   aaCTG‑AAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTT‑CACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTg                                                    >  1:2512447/1‑150 (MQ=255)
                                                                                                    aCTG‑AAATCCCAGAAAAAGGGGGCTGTGGATGCAGGTAATTTTCCCCT‑CCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTgg                                                   <  1:1180395/150‑1 (MQ=255)
                                                                                                     cTG‑AAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTT‑CCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGc                                                  <  1:3429949/150‑1 (MQ=255)
                                                                                                      tG‑AAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTCCCTT‑CACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGcc                                                 <  1:2599702/150‑1 (MQ=255)
                                                                                                      tG‑AAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTT‑CACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGcc                                                 >  1:1783070/1‑150 (MQ=255)
                                                                                                       g‑AAACCCCAGCAAAAGGGGGCTGTGGATGCAGCGAAATTTACCTC‑CCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCcg                                                <  2:1220908/150‑1 (MQ=255)
                                                                                                              ccAGCAAAATGGGGCTGTGGATGCAGCGAAATTTCCCTT‑CCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTa                                          <  1:2726092/150‑1 (MQ=255)
                                                                                                                 gCAAAATGGGGCTGTGGATGCAGCGAAATTTCCCTT‑CCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGc                                       <  1:2205108/150‑1 (MQ=255)
                                                                                                                   aaaaTGGGGCTGTGGATGCAGCGAAATTTACCTT‑CACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCgg                                     >  2:2502995/1‑150 (MQ=255)
                                                                                                                   aaaaTGGGGCTGTGGATGCAGCGAAATTTACCTT‑CACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCgg                                     >  2:2354821/1‑150 (MQ=255)
                                                                                                                   aaaaTGGGGCTGTGGATGCAGCGAAATTTACCTT‑CACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCgg                                     >  1:2890902/1‑150 (MQ=255)
                                                                                                                    aaaTGGGGCTGTGGATGCAGCGAAATTTCCCTT‑CCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGc                                    <  1:163684/150‑1 (MQ=255)
                                                                                                                       tGGGGTTGTGGATGCAGCGAAATTTCCCTC‑CCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCAg                                 <  1:1396282/150‑1 (MQ=255)
                                                                                                                         gggCTGTGGATGCAGCGAAATTTCCCTC‑CACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCAGaa                               <  1:795859/150‑1 (MQ=255)
                                                                                                                          ggCTGTGGATGCAGCGAAATTTCCCTT‑CCCCCCGCCGCAAGGCGTCACGGTAGACGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCAGaaa                              <  2:3837789/150‑1 (MQ=255)
                                                                                                                          ggCTGTGGATGCAGCGAAATTTACCTT‑CACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCAGaaa                              >  1:320689/1‑150 (MQ=255)
                                                                                                                             tgtgGATGCAGCGAAATTTACCTT‑CACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCAGAAAAtt                           >  1:129334/1‑150 (MQ=255)
                                                                                                                              gtgGATGCAGCGAAATTTACCTT‑CCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCAGAAAAttt                          <  1:3661884/150‑1 (MQ=255)
                                                                                                                                ggATGCAGCGAAATTTACCTT‑CACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCAGAAAATTTAg                        <  1:680586/150‑1 (MQ=255)
                                                                                                                                    gcagcaAAATTTCCCTT‑CCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCAGAAAATTTAGcaca                    <  2:3182318/150‑1 (MQ=255)
                                                                                                                                      aGCGAAATTACCCTC‑CACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCAGAAAATTTAGCACAGt                  <  1:2044108/150‑1 (MQ=255)
                                                                                                                                       gCGAAATTTACCTT‑CACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCAGAAAATTTAGCACAGta                 >  2:230877/1‑150 (MQ=255)
                                                                                                                                        cGAATTTTCCCTT‑CACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCAGAAAATTTAGCACAGtat                <  2:3929529/150‑1 (MQ=255)
                                                                                                                                        cGAAATTTCCCTT‑CACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCAGAAAATTTAGCACAGtat                <  2:1814640/150‑1 (MQ=255)
                                                                                                                                        cGAAATTTACCTT‑CACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCAGAAAATTTAGCACAGtat                >  2:1010695/1‑150 (MQ=255)
                                                                                                                                          aattttACCTA‑CGCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCAGAAAATTCAGCACAGTatat              <  2:4096311/147‑1 (MQ=255)
                                                                                                                                           aaTTTACCTT‑CACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCAGAAAATTTAGCACAGTATATc             >  1:2651844/1‑150 (MQ=255)
                                                                                                                                            aTTTACCTT‑CCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCAGAAAATTTAGCACAGTATATCg            <  2:1240817/150‑1 (MQ=255)
                                                                                                                                            aTTTACCTT‑CACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCAGAAAATTTAGCACAGTATATCg            >  1:428515/1‑150 (MQ=255)
                                                                                                                                            aTTTACCTT‑CACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCAGAAAATTTAGCACAGTATATCg            <  1:2472748/150‑1 (MQ=255)
                                                                                                                                              ttACCTT‑CACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCAGAAAATTTAGCACAGTATATCGGc          >  2:595534/1‑150 (MQ=255)
                                                                                                                                                aCCTT‑CACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCAGAAAATTTAGCACAGTATATCGGCCa        <  1:3751245/150‑1 (MQ=255)
                                                                                                                                                   tt‑CACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCAGAAAATTTAGCACAGTATATCGGCCAGCa     >  2:881231/1‑150 (MQ=255)
                                                                                                                                                   tt‑CACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCAGAAAATTTAGCACAGTATATCGGCCAGCa     >  2:3088886/1‑150 (MQ=255)
                                                                                                                                                    t‑CACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCAGAAAATTTAGCACAGTATATCGGCCAGCaa    >  2:1748839/1‑150 (MQ=255)
                                                                                                                                                       |                                                                                                                                                     
GCAATGGCAATCTGAAGCAGTTCACCATTAACGTGGGACGTGATGGCACAATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTG‑AAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTT‑CACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCAGAAAATTTAGCACAGTATATCGGCCAGCAACA  >  AP012306/800526‑800824

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑