Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123061,128,4120TG24.5% 747.4 / 54.0 296V29G (GTG→GGG) yciOconserved protein
Reads supporting (aligned to +/- strand):  ref base T (50/172);  new base G (72/0);  total (124/172)
Fisher's exact test for biased strand distribution p-value = 8.24e-36
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 344 total aligned reads displayed.

CCGCAGGCGTTGAAGGCGTCTGGCAGCTATGGGAACAGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTG‑TTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTCGCGATCTTTCTGAACTCTCGACCTATT  >  AP012306/1128263‑1128561
                                                                                                                                                     |                                                                                                                                                      
ccGCAGGCGTTGAAGGCGTCTGGCAGCTATGGGAACAGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGTGGt                                                                                                                                                        <  2:3586389/150‑1 (MQ=255)
ccGCAGGCGTTGAAGGCGTCTGGCAGCTATGGGAACAGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGt                                                                                                                                                        >  2:1267598/1‑150 (MQ=255)
   cAGGCGTTGAAGGCGTCTGGCAGCTATGGGAACAGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGAt                                                                                                                                                     <  1:3304026/150‑1 (MQ=255)
        gTTGAAGGCGTCTGGCAGCTATGGGAACAGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTG‑ttt                                                                                                                                               <  2:1616004/150‑1 (MQ=255)
        gTTGAAGGCGTCTGGCAGCTATGGGAACAGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTG‑ttt                                                                                                                                               <  1:2609569/150‑1 (MQ=255)
          tGAAGGCGTCTGGCAGCTATGGGAACAGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTG‑TTTAt                                                                                                                                             <  1:1393581/150‑1 (MQ=255)
            aaGGCGTCTGGCAGCTATGGGAACAGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTG‑TTTATcc                                                                                                                                           >  1:340807/1‑150 (MQ=255)
             aGGCGTCTGGCAGCTATGGGAACAGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGATTG‑TTTATCCa                                                                                                                                          >  1:3216628/1‑150 (MQ=255)
              ggCGTCTGGCAGCTATGGGAACAGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTG‑TTTATCCaa                                                                                                                                         <  2:1422414/150‑1 (MQ=255)
                 gTCTGGCAGCTATGGGAACAGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTG‑TTTATCCAACTg                                                                                                                                      <  2:3991703/150‑1 (MQ=255)
                   cTGGCAGCTATGGGAACAGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTG‑TTTATCCAACTGAt                                                                                                                                    <  2:2139399/150‑1 (MQ=255)
                       cAGCTATGGGAACAGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTG‑TTTATCCAACTGATTCCg                                                                                                                                <  2:2604950/150‑1 (MQ=255)
                           tATGGGAACAGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGATTG‑TTTATCCAACTGATTCCGGCTa                                                                                                                            >  2:2297096/1‑150 (MQ=255)
                            aTGGGAACAGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTG‑TTTATCCAACTGATTCCGGCTAt                                                                                                                           <  2:924813/150‑1 (MQ=255)
                             tGGGAACAGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTG‑TTTATCCAACTGATTCCGGCTATg                                                                                                                          <  2:1784825/150‑1 (MQ=255)
                             ggggAACAGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTG‑TTTATCCAACTGATTCCGGCTATg                                                                                                                          <  2:1065728/149‑1 (MQ=255)
                              gggAACAGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTG‑TTTATCCAACTGATTCCGGCTATgc                                                                                                                         >  1:3177859/1‑150 (MQ=255)
                                gAACAGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGGTTG‑TTTATCCAACTGATTCCGGCTATgcgc                                                                                                                       >  2:2225071/1‑150 (MQ=255)
                                   cAGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTG‑TTTATCCAACTGATTCCGGCTATGCGCTCg                                                                                                                    <  1:1005023/150‑1 (MQ=255)
                                    aGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTG‑TTTATCCAACTGATTCCGGCCATGCGCTCgg                                                                                                                   >  1:3651184/1‑150 (MQ=255)
                                           gAACACCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTG‑TTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTaaa                                                                                                            <  1:737420/150‑1 (MQ=255)
                                            aaCACCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTG‑TTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTaaaa                                                                                                           <  1:3728708/150‑1 (MQ=255)
                                            aaCACCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGGTTG‑TTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTaaaa                                                                                                           >  2:3569853/1‑150 (MQ=255)
                                             acacCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTG‑TTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAAt                                                                                                          <  1:2552300/150‑1 (MQ=255)
                                              caccacAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGGTCGTGCGTAAAGGCGGGGGGGGTG‑TTTTTCCAACTGATTTCGGCTATGCGCTCCGCTGTGAAAtt                                                                                                         >  2:1972426/1‑150 (MQ=255)
                                              caccacAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTG‑TTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAAtt                                                                                                         <  1:2793798/150‑1 (MQ=255)
                                              caccacAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGGTTG‑TTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAAtt                                                                                                         >  2:3297021/1‑150 (MQ=255)
                                               accacAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGATTG‑TTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTg                                                                                                        >  1:313659/1‑150 (MQ=255)
                                               accacAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGATTG‑TTTATCCAACTGATTCCGGCTATGCGCTCGGCCGTGAAATTg                                                                                                        >  2:779584/1‑150 (MQ=255)
                                                ccacAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTG‑TTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTGa                                                                                                       >  1:3912579/1‑150 (MQ=255)
                                                ccacAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTG‑TTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTGa                                                                                                       <  1:3341651/150‑1 (MQ=255)
                                                 cacaGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTG‑TTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTGaa                                                                                                      <  2:2491728/150‑1 (MQ=255)
                                                 cacaGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGGTTGTTTTTTCCAACGAATTCCGGCTTTGCGCTCGCCTGTAAAATTGa                                                                                                       >  2:2017633/1‑150 (MQ=255)
                                                      agagGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTG‑TTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGATaa                                                                                                 <  1:643885/150‑1 (MQ=255)
                                                       gagGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTG‑TTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGATaaa                                                                                                <  1:2739787/150‑1 (MQ=255)
                                                        agGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTG‑TTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGATaaaa                                                                                               <  1:1420438/150‑1 (MQ=255)
                                                         gggAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGT‑AAGGCGGGGTGATTG‑TTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGATAAAAAc                                                                                             <  1:3055131/150‑1 (MQ=255)
                                                          ggAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTG‑TTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGATAAAAAc                                                                                             <  1:4186780/150‑1 (MQ=255)
                                                          ggAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTG‑TTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGATAAAAAc                                                                                             <  1:4049012/150‑1 (MQ=255)
                                                          ggAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGATTG‑TTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGATaaaaaa                                                                                             >  2:808253/1‑149 (MQ=255)
                                                          ggAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGATTG‑TTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGATAAAAAc                                                                                             >  1:1056124/1‑150 (MQ=255)
                                                            aaTTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTG‑TTTATCCAACTGATTCCGGCTATGGGCTCGGCTGTAAAATTGAAGATAAAAAcgc                                                                                           >  2:1553871/1‑150 (MQ=255)
                                                             aTTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTG‑TTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGATAAAAAcgcg                                                                                          >  1:2668050/1‑150 (MQ=255)
                                                               tATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGATTG‑TTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGATAAAAAAGCGAt                                                                                        >  2:2362320/1‑150 (MQ=255)
                                                                aTGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGATTG‑TTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAAATGAAGATAAAAACGCGATg                                                                                       >  1:1770782/1‑150 (MQ=255)
                                                                      cAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTG‑TTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGATAAAAACGCGATGGAGCGt                                                                                 >  1:1643819/1‑150 (MQ=255)
                                                                      cAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTG‑TTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGATAAAAACGCGATGGAGCGt                                                                                 <  2:1315942/150‑1 (MQ=255)
                                                                      cAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTG‑TTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGATAAAAACGCGATGGAGCGt                                                                                 <  1:84521/150‑1 (MQ=255)
                                                                        gTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTG‑TTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGATAAAAACGCGATGGAGCGTAt                                                                               <  1:1922392/150‑1 (MQ=255)
                                                                         tttttttATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGGTTG‑TTTTTCCAACTGATTCCGGCTTTGCGCTCGGCTGTAAAATTGAAGATAAAAACGCGGTGGAGCGTAtt                                                                              >  2:2537056/1‑150 (MQ=255)
                                                                            ttttATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCATAAAGGCGGGGTGATTG‑TTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGt                                                                           <  1:1097807/150‑1 (MQ=255)
                                                                               tataTTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTG‑TTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGt                                                                        <  1:2391698/150‑1 (MQ=255)
                                                                                   ttCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGAATG‑TTTATCCAACTGATTTCGGCTATGCGCTCGGGTGTAAAATTGAAGATAAAAACGCGATTGAGCGTATTTTTCGTATTc                                                                    >  2:2330014/1‑150 (MQ=255)
                                                                                     cATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGATTG‑TTTATCCAACTGGTTCCGGCTATTCGCCCGGCTGTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTccc                                                                  >  2:3801144/1‑148 (MQ=255)
                                                                                      aTCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTG‑TTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGcc                                                                 <  2:3228331/150‑1 (MQ=255)
                                                                                      aTCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTG‑TTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGATAAAAACGCGATGGAGCGGATTTTTCGTATTCGcc                                                                 >  1:2185826/1‑150 (MQ=255)
                                                                                       tCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTG‑TTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTTAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCa                                                                >  1:3287120/1‑150 (MQ=255)
                                                                                         cTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGGGATCGTGCGTAAAGGCGGGGGGATTG‑TTTTTCCAACTGGTTCCGGGTATGCGCTCGGGTGGAAAATTTAAGATAAAAACGCGATGGGGGGGATTTGTCGTATTCGCCCGc                                                              >  2:3643362/1‑150 (MQ=255)
                                                                                         cTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGATTG‑TTTATCCAACTGATTCCGGCTATGCGCTCGGCTGGAAAATTGAAGATAAAAACGCGATGGGGCGTATTTTTCGTATTCGCCAGc                                                              >  2:629626/1‑150 (MQ=255)
                                                                                          tGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTG‑TTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCt                                                             <  1:1728261/150‑1 (MQ=255)
                                                                                          tGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTG‑TTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGATAAAAACGCGATGGAGCGGATTTGTCGGATTCGCCAGCt                                                             >  1:581017/1‑150 (MQ=255)
                                                                                           gATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTG‑TTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTg                                                            <  1:2140933/150‑1 (MQ=255)
                                                                                            aTAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGATTG‑TTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGATAAAAACGCGGTGGAGCGTATTTGTCGGATTCGCCAGCTGc                                                           >  2:113508/1‑150 (MQ=255)
                                                                                             tAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTG‑TTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGcc                                                          <  1:2878137/150‑1 (MQ=255)
                                                                                             tAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGATTG‑TTTATCCAAATGATTCCGGCTATGCGCTCGGCTGTAAAATTTAAGATAAAAAAGCGATGGGGCGGATTTGTCGTATTCGCCAGCTGcc                                                          >  2:578697/1‑150 (MQ=255)
                                                                                                cccACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGATTG‑TTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGa                                                       >  1:212079/1‑150 (MQ=255)
                                                                                                   acaGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTG‑TTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACgg                                                    >  1:2303129/1‑150 (MQ=255)
                                                                                                   acaGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGATTG‑TTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACgg                                                    >  1:1299873/1‑150 (MQ=255)
                                                                                                     aGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTG‑TTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTc                                                  <  2:4180540/150‑1 (MQ=255)
                                                                                                          taaacaaTCAACAAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTG‑TTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAAc                                             >  2:856106/7‑150 (MQ=255)
                                                                                                            tCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTG‑TTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACtt                                           <  1:3859163/150‑1 (MQ=255)
                                                                                                             cTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGATTG‑TTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACttt                                          >  1:3741810/1‑150 (MQ=255)
                                                                                                             cTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGATTG‑TTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGGCACAACttt                                          >  1:3520596/1‑150 (MQ=255)
                                                                                                              tGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTG‑TTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTa                                         <  1:977072/150‑1 (MQ=255)
                                                                                                              tGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTG‑TTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTa                                         >  2:1106911/1‑150 (MQ=255)
                                                                                                              tGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGATTG‑TTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGATAAAAACGCGATGGAGCGGATTTGTCGTATTCGCCAGCTGCCGGACGGGCACAACTTTa                                         >  2:3150612/1‑150 (MQ=255)
                                                                                                                  cAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTG‑TTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCt                                     <  1:457715/150‑1 (MQ=255)
                                                                                                                    aCCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTG‑TTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGa                                   <  2:2952586/150‑1 (MQ=255)
                                                                                                                        ggCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTG‑TTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGAtgtg                               <  2:3455880/150‑1 (MQ=255)
                                                                                                                         gCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTG‑TTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATgtgt                              <  1:2916972/150‑1 (MQ=255)
                                                                                                                          cGGTGGAGATCGTGCGTAAAGGCGGGGTGATTG‑TTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTc                             <  1:3763415/150‑1 (MQ=255)
                                                                                                                               gagaTCGTGCGTAAAGGCGGGGTGATTG‑TTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTCGCGAt                        <  2:284405/150‑1 (MQ=255)
                                                                                                                                agaTCGTGCGTAAAGGCGGGGTGATTG‑TTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTCGCGATc                       <  1:3643206/150‑1 (MQ=255)
                                                                                                                                 gaTCGTGCGTAAAGGCGGGGTGATTG‑TTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTCGCGATCt                      <  1:2997644/150‑1 (MQ=255)
                                                                                                                                 gaTCGTGCGTAAAGGCGGGGTGATTG‑TTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTCGCGATCt                      <  2:2636513/150‑1 (MQ=255)
                                                                                                                                  aTCGTGCGTAAAGGCGGGGTGATTG‑TTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTCGCGATCtt                     <  2:3715282/150‑1 (MQ=255)
                                                                                                                                     gTGCGTAAAGGCGGGGTGATTG‑TTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTCGGGGTCTTTCt                  >  2:169054/1‑150 (MQ=255)
                                                                                                                                       gCGTAAAGGCGGGGTGATTG‑TTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTCGCGATCTTTCTGa                <  1:2471094/150‑1 (MQ=255)
                                                                                                                                         gTAAAGGCGGGGTGATTG‑TTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTCGCGGTCTTTCTGAAc              >  2:2054134/1‑150 (MQ=255)
                                                                                                                                         gTAAAGGCGGGGTGATTG‑TTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTCGCGATCTTTCTGAAc              <  2:338369/150‑1 (MQ=255)
                                                                                                                                            aaGGCGGGGTGATTG‑TTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTCGCGATCTTTCTGAActct           >  2:2674415/1‑150 (MQ=255)
                                                                                                                                             aGGCGGGGTGATTG‑TTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTCGCGATCTTTCTGAACtctc          >  2:3084814/1‑150 (MQ=255)
                                                                                                                                               gCGGGGTGATTG‑TTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTCGCGATCTTTCTGAACTCTCGa        <  1:489545/150‑1 (MQ=255)
                                                                                                                                                 ggggTGATTG‑TTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTCGCGATCTTTCTGAACTCTCGAcc      <  1:816887/150‑1 (MQ=255)
                                                                                                                                                 ggggTGATTG‑TTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTCGCGATCTTTCTGAACTCTCGAcc      <  1:3386015/150‑1 (MQ=255)
                                                                                                                                                  gggTGATTG‑TTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTCGCGATCTTTCTGAACTCTCGACCt     <  1:1691804/150‑1 (MQ=255)
                                                                                                                                                  gggTGATTG‑TTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTCGCGATCTTTCTGAACTCTCGACCt     <  2:189157/150‑1 (MQ=255)
                                                                                                                                                     tGATTG‑TTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTCGCGATCTTTCTGAACTCTCGACCTAtt  <  2:3949173/150‑1 (MQ=255)
                                                                                                                                                     tGATTG‑TTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTCGCGATCTTTCTGAACTCTCGACCTAtt  <  1:1462912/150‑1 (MQ=255)
                                                                                                                                                     tGATTG‑TTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTCGCAATCTTTCTGAACTCTCGACCTAtt  >  1:1520215/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                      
CCGCAGGCGTTGAAGGCGTCTGGCAGCTATGGGAACAGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTG‑TTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTCGCGATCTTTCTGAACTCTCGACCTATT  >  AP012306/1128263‑1128561

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑