Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123061,393,3790AC24.0% 672.7 / 34.7 242T92P (ACC→CCC) nthDNA glycosylase and apyrimidinic (AP) lyase
Reads supporting (aligned to +/- strand):  ref base A (166/18);  new base C (0/58);  total (166/76)
Fisher's exact test for biased strand distribution p-value = 2.77e-40
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 276 total aligned reads displayed.

CGACCGATGTCAGTGTTAATAAGGCGACGGCGAAACTCTACCCGGTGGCGAATACGCCTGCAGCGATGCTTGAACTGGGCGTTGAAGGGGTGAAAACCTATATCAAAACGATTGGGCTTTATAACAGCAAAGCAGAAAATATCATCAAAACCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGTGCTGCGCTTGAAGCCCTGCCCGGCGTAGGTCGTAAAACAGCCAACGTCGTATTAAACACTGCATTCGGCTGGCCGACTATTGCTGTCGACACGCACAT  >  AP012306/1393230‑1393527
                                                                                                                                                     |                                                                                                                                                    
cGACCGATGTCAGTGTTAATAAGGCGACGGCGAAACTCTACCCGGTGGCGAATACGCCTGCAGCGATGCTTGAACTGGGCGTTGAAGGGGTGAAAACCTATATCAAAACGATTGGGCTTTATAACAGCAAAGCAGAAAATATCATCaaaa                                                                                                                                                      <  1:1507326/150‑1 (MQ=255)
 gACCGATGTCAGTGTTAATAAGGCGACGGCGAAACTCTACCCGGTGGCGAATACGCCTGCAGCGATGCTTGAACTGGGCGTTGAAGGGGTGAAAACCTATATCAAAACGATTGGGCTTTATAACAGCAAAGCAGAAAATATCATCAAAAc                                                                                                                                                     >  2:2102758/1‑150 (MQ=255)
     gATGTCAGTGTTAATAAGGCGACGGCGAAACTCTACCCGGTGGCGAATACGCCTGCAGCGATGCTTGAACTGGGCGTTGAAGGGGTGAAAACCTATATCAAAACGATTGGGCTTTATAACAGCAAAGCAGAAAATATCATCAAAACCTGc                                                                                                                                                 >  2:4072514/1‑150 (MQ=255)
     gATGTCAGTGTTAATAAGGCGACGGCGAAACTCTACCCGGTGGCGAATACGCCTGCAGCGATGCTTGAACTGGGCGTTGAAGGGGTGAAAACCTATATCAAAACGATTGGGCTTTATAACAGCAAAGCAGAAAATATCATCAAAACCTGc                                                                                                                                                 <  2:3066902/150‑1 (MQ=255)
       tGTCAGTGTTAATAAGGCGACGGCGAAACTCTACCCGGTGGCGAATACGCCTGCAGCGATGCTTGAACTGGGCGTTGAAGGGGTGAAAACCTATATCAAAACGATTGGGCTTTATAACAGCAAAGCAGAAAATATCATCAAAACCTGCCg                                                                                                                                               <  1:2863625/150‑1 (MQ=255)
       tGTCAGTGTTAATAAGGCGACGGCGAAACTCTACCCGGTGGCGAATACGCCTGCAGCGATGCTTGAACTGGGCGTTGAAGGGGTGAAAACCTATATCAAAACGATTGGGCTTTATAACAGCAAAGCAGAAAATATCATCAAAACCTGCCg                                                                                                                                               >  1:1770055/1‑150 (MQ=255)
        gTCAGTGTTAATAAGGCGACGGCGAAACTCTACCCGGTGGCGAATACGCCTGCAGCGATGCTTGAACTGGGCGTTGAAGGGGTGAAAACCTATATCAAACCGATTGGGCTTTATAACAGCAAAGCAGAAAATATCATCAAAACCTGCCGt                                                                                                                                              <  2:15606/150‑1 (MQ=255)
          cAGTGTTAATAAGGCGACGGCGAAACTCTACCCGGTGGCGAATACGCCTGCAGCGATGCTTGAACTGGGCGTTGAAGGGGTGAAAACCTATATCAAAACGATTGGGCTTTATAACAGCAAAGCAGAAAATATCATCAAAACCTGCCGTAt                                                                                                                                            >  2:3158390/1‑150 (MQ=255)
           aGTGTTAATAAGGCGACGGCGAAACTCTACCCGGTGGCGAATACGCCTGCAGCGATGCTTGAACTGGGCGTTGAAGGGGTGAAAACCTATATCAAAACGATTGGGCTTTATAACAGCAAAGCAGAAAATATCATCAAAACCTGCCGTATc                                                                                                                                           >  2:2533831/1‑150 (MQ=255)
             tgtTAATAAGGCGACGGCGAAACTCTACCCGGTGGCGAATACGCCTGCAGCGATGCTTGAACTGGGCGTTGAAGGGGTGAAAACCTATATCAAAACGATTGGGCTTTATAACAGCAAAGCAGAAAATATCATCAAAACCTGCCGTATCtt                                                                                                                                         >  1:863882/1‑150 (MQ=255)
              gtTAATAAGGCGACGGCGAAACTCTACCCGGTGGCGAATACGCCTGCAGCGATGCTTGAACTGGGCGTTGAAGGGGTGAAAACCTATATCAAAACGATTGGGCTTTATAACAGCAAAGCAGAAAATATCATCAAAACCTGCCGTATCTTg                                                                                                                                        >  2:596543/1‑150 (MQ=255)
                taataaGGCGACGGCGAAACTCTACCCGGTGGCGAATACGCCTGCAGCGATGCTTGAACTGGGCGTTGAAGGGGTGAAAACCTATATCAAAACGATTGGGCTTTATAACAGCAAAGCAGAAAATATCATCAAAACCTGCCGTATCTTGCt                                                                                                                                      >  1:4019307/1‑150 (MQ=255)
                   taaGGCGACGGCGAAACTCTACCCGGTGGCGAATACGCCTGCAGCGATGCTTGAACTGGGCGTTGAAGGGGTGAAAACCTATATCAAAACGATTGGGCTTTATAACAGCAAAGCAGAAAATATCATCAAAACCTGCCGTATCTTGCTGGa                                                                                                                                   >  1:1960106/1‑150 (MQ=255)
                      ggCGACGGAAAAACTCTCCCGGGTGGCGAATACCCCGGCAGCGATGCTTGAACTGGGCGTGGAGGGGGTGAAAACCTATATAAAAACGATTGGGTTTTATAACAGAAAAGAAAAAAATATCATAAAAACCTGCCGTATCTTGCTGGagca                                                                                                                                <  2:3890606/150‑1 (MQ=255)
                          aCGGCGAACCTCTCCCGGGTGGCAAATCCGCCTGCAGCGATGCTTGAACTGGGCGTTGAAGGGGTAAAAACCTATATAAAAACGTTTGGGCTTTATAACAGCAAAGAAAAAAATATCAACAAAACCTGCCGTATCTTGCTGGAGCAGCAt                                                                                                                            <  2:792916/150‑1 (MQ=255)
                          aCGGCGAAACTCTACCCGGTGGCGAATACGCCTGCAGCGATGCTTGAACTGGGCGTTGAAGGGGTGAAAACCTATATCAAAACGATTGGGCTTTATAACAGCAAAGCAGAAAATATCATCAAAACCTGCCGTATCTTGCTGGAGCAGCAt                                                                                                                            >  1:1211773/1‑150 (MQ=255)
                            ggCGAAACTCTCCCGGGTGGCGAATACGCCTGCAGCGATGCTTGAACTGGGCGTTGAAGGGGTGAAAACCTATATCAAAACGATTGGGCTTTATAACAGCAAAGCAGAAAATATCATAAAAACCTGCCGTATCTTGCTGGAGCAGCATaa                                                                                                                          <  1:1816148/150‑1 (MQ=255)
                              cGAAACTCTACCCGGTGGCGAATACGCCTGCAGCGATGCTTGAACTGGGCGTTGAAGGGGTGAAAACCTATATCAAAACGATTGGGCTTTATAACAGCAAAGCAGAAAATATCATCAAAACCTGCCGTATCTTGCTGGAGCAGCATAATg                                                                                                                        >  2:3741386/1‑150 (MQ=255)
                               gAAACTCTACCCGGTGGCGAATACGCCTGCAGCGATGCTTGAACTGGGCGTTGAAGGGGTGAAAACCTATATCAAAACGATTGGGCTTTATAACAGCAAAGCAGAAAATATCATCAAAACCTGCCGTATCTTGCTGGAGCAGCATAATgg                                                                                                                       >  1:1868877/1‑150 (MQ=255)
                               gAAACTCTACCCGGTGGCGAATACGCCTGCAGCGATGCTTGAACTGGGCGTTGAAGGGGTGAAAACCTATATCAAAACGATTGGGCTTTATAACAGCAAAGCAGAAAATATCATCAAAACCTGCCGTATCTTGCTGGAGCAGCATAATgg                                                                                                                       >  1:4135617/1‑150 (MQ=255)
                               aaacccctACCGGGTGGCAAATACGCCTCCAGCGATGTTTAAACTGGGCGTGGAAGGGGTAAAACCCAATACAAAAACGATTGGTCTTTAAAAAAGCAAGGAAAAAAAAATCATCAAACCCTGCCGTATCTTGCTGGAGCAGCATAATgg                                                                                                                       <  2:483055/144‑1 (MQ=255)
                                aaaCTCTACCCGGTGGCGAATACGCCTGCAGCGATGCTTGAACTGGGCGTTGAAGGGGTGAAAACCTATATCAAAACGATTGGGCTTTATAACAGCAAAGCAGAAAATATCATCAAAACCTGCCGTATCTTGCTGGAGCAGCATAATGGc                                                                                                                      >  2:2002948/1‑150 (MQ=255)
                                  aCTCTACCCGGTGGCGAATACGCCTGCAGCGATGCTTGAACTGGGCGTTGAAGGGGTGAAAACCTATATCAAAACGATTGGGCTTTATAACAGCAAAGCAGAAAATATCATCAAAACCTGCCGTATCTTGCTGGAGCAGCATAATGGCGa                                                                                                                    >  1:3714992/1‑150 (MQ=255)
                                     ctACCCGGTGGCGAATACGCCTGCAGCGATGCTTGAACTGGGCGTTGAAGGGGTGAAAACCTATATCAAAACGATTGGGCTTTATAACAGCAAAGCAGAAAATATCATCAAAACCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGt                                                                                                                 >  1:1578588/1‑150 (MQ=255)
                                      tACCCGGTGGCGAATACGCCTGCAGCGATGCTTGAACTGGGCGTTGAAGGGGTGAAAACCTATATCAAAACGATTGGGCTTTATAACAGCAAAGCAGAAAATATCATCAAAACCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGtt                                                                                                                >  2:2332037/1‑150 (MQ=255)
                                      tACCCGGTGGCGAATACGCCTGCAGCGATGCTTGAACTGGGCGTTGAAGGGGTGAAAACCTATATCAAAACGATTGGGCTTTATAACAGCAAAGCAGAAAATATCATCAAAACCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGtt                                                                                                                >  2:271396/1‑150 (MQ=255)
                                       aCCCGGTGGCGAATACGCCTGCAGCGATGCTTGAACTGGGCGTTGAAGGGGTGAAAACCTATATCAAAACGATTGGGCTTTATAACAGCAAAGCAGAAAATATCATCAAAACCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTc                                                                                                               >  2:3775641/1‑150 (MQ=255)
                                          cGGTGGCGAATACGCCTGCAGCGATGCTTGAACTGGGCGTTGAAGGGGTGAAAACCTATATCAAAACGATTGGGCTTTATAACAGCAAAGCAGAAAATATCATCAAAACCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCgg                                                                                                            >  1:1344672/1‑150 (MQ=255)
                                           ggTGGCGAATACGCCTGCAGCGATGCTTGAACTGGGCGTTGAAGGGGTGAAAACCTATATCAAAACGATTGGGCTTTATAACAGCAAAGCAGAAAATATCATCAAAACCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGa                                                                                                           >  1:652757/1‑150 (MQ=255)
                                           ggTGGCGAATACGCCTGCAGCGATGCTTGAACTGGGCGTTGAAGGGGTGAAAACCTATATCAAAACGATTGGGCTTTATAACAGCAAAGCAGAAAATATCATCAAAACCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGa                                                                                                           >  2:3932900/1‑150 (MQ=255)
                                                 gAATACGCCTGCAGCGATGCTTGAACTGGGCGTTGAAGGGGTGAAAACCTATATCAAAACGATTGGGTTTTATAACAGCAAAGAAGAAAATATCATCAAACCCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCg                                                                                                     <  1:3119108/150‑1 (MQ=255)
                                                   aTACGCCTGCAGCGATGCTTGAACTGGGCGTTGAAGGGGTGAAAACCTATATCAAAACGATTGGGCTTTATAACAGCAAAGCAGAAAATATCATCAAAACCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGtg                                                                                                   >  2:2392564/1‑150 (MQ=255)
                                                   aTACGCCTGCAGCGATGCTTGAACTGGGCGTTGAAGGGGTGAAAACCTATATCAAAACGATTGGGCTTTATAACAGCAAAGCAGAAAATATCATCAAAACCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGtg                                                                                                   >  2:847652/1‑150 (MQ=255)
                                                    tACGCCTGCAGCGATGCTTGAACTGGGCGTTGAAGGGGTGAAAACCTATATCAAAACGATTGGGCTTTATAACAGCAAAGCAGAAAATATCATCAAAACCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGtgc                                                                                                  >  2:760074/1‑150 (MQ=255)
                                                      cGCCTGCAGCGATGCTTGAACTGGGCGTTGAAGGGGTGAAAACCTATATCAAAACGATTGGGCTTTATAACAGCAAAGCAGAAAATATCATCAAAACCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGtgctg                                                                                                >  1:2556228/1‑150 (MQ=255)
                                                       gCCTGCAGCGATGCTTGAACTGGGCGTTGAAGGGGTGAAAACCTATATCAAAACGATTGGGCTTTATAACAGCAAAGCAGAAAATATCATCAAAACCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGtgctgc                                                                                               >  2:3900519/1‑150 (MQ=255)
                                                         cTGCAGCGATGCTTGAACTGGGCGTTGAAGGGGTAAAAACCTATATAAAAACGATTGGGCTTTATAACAGCAAAGCAGAAAATATCACCAAAACCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGTGCTgcgc                                                                                             <  2:2595645/150‑1 (MQ=255)
                                                                 aggCTTGAACTGGGCGTTGAAGGGGTGAAAACCTATATAAAAACGATTGGGCTTTATAACAGAAAAGCAAAAAATACCATCAAACCCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGTGCTGCGCTTGAAGcc                                                                                     <  2:4040666/148‑1 (MQ=255)
                                                                      tGAACTGGGCGTTGAAGGGGTGAAAACCTATATCAAAACGATTGGGCTTTATAACAGCAAAGCAGAAAATATCATCAAAACCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGTGCTGCGCTTGAAGCCCTGcc                                                                                >  1:2688349/1‑150 (MQ=255)
                                                                       gAACTGGGCGTTGAAGGGGTGAAAACCTATATCAAAACGATTGGGCTTTATAACAGCAAAGCAGAAAATATCATCAAAACCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGTGCTGCGCTTGAAGCCCTGccc                                                                               >  1:4100289/1‑150 (MQ=255)
                                                                       gAACGGGGCGTGGAGGGGGTAAAACCTTATATAAAAACGATTGGGTTTTATAACAGAAAAGAAGAAAATATCAACAAACCCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGTGCTGCGCTTGAAGCCCTGccc                                                                               <  2:1003968/150‑1 (MQ=255)
                                                                        aaCTGGGCGTTGAAGGGGTGAAAACCTATATCAAAACGATTGGGCTTTATAACAGCAAAGCAGAAAATATCATCAAAACCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGTGCTGCGCTTGAAGCCCTGCCCg                                                                              >  2:1177676/1‑150 (MQ=255)
                                                                         aCTGGGCGTTGAAGGGGTGAAAACCTATATCAAAACGATTGGGCTTTATAACAGCAAAGCAGAAAATATCATCAAAACCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGTGCTGCGCTTGAAGCCCTGCCCgg                                                                             >  2:4158192/1‑150 (MQ=255)
                                                                           tGGGCGTTGAAGGGGTAAAAACTTATATAAAAACGATTGGGCTTTATAACAGAAAAGAAGAAAATATCATAAAACCCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGTGCTGCGCTTGAAGCCCTGCCCGGCg                                                                           <  2:726161/150‑1 (MQ=255)
                                                                            gggCGTTGAAGGGGTGAAAACCTATATCAAAACGATTGGGCTTTATAACAGCAAAGCAGAAAATATCATCAAAACCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGTGCTGCGCTTGAAGCCCTGCCCGGCGt                                                                          >  2:91760/1‑150 (MQ=255)
                                                                            gggCGTTGAAGGGGTGAAAACCTATATCAAAACGATTGGGCTTTATAACAGCAAAGCAGAAAATATCATCAAAACCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGTGCTGCGCTTGAAGCCCTGCCCGGCGt                                                                          >  1:2045958/1‑150 (MQ=255)
                                                                                 ttGAAGGGGTGAAAACCTATATCAAAACGATTGGGCTTTATAACAGCAAAGCAGAAAATATCATCAAAACCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGTGCTGCGCTTGAAGCCCTGCCCGGCGTAGGTc                                                                     >  1:4051035/1‑150 (MQ=255)
                                                                                 ttGAAGGGGTGAAAACCTATATCAAAACGATTGGGCTTTATAACAGCAAAGCAGAAAATATCATCAAAACCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGTGCTGCGCTTGAAGCCCTGCCCGGCGTAGGTc                                                                     >  2:2449015/1‑150 (MQ=255)
                                                                                  tGAAGGGGTGAAAACCTATATCAAAACGATTGGGCTTTATAACAGCAAAGCAGAAAATATCATCAAAACCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGTGCTGCGCTTGAAGCCCTGCCCGGCGTAGGTCg                                                                    >  2:1876053/1‑150 (MQ=255)
                                                                                  tGAAGGGGTGAAAACCTATATCAAAACGATTGGGCTTTATAACAGCAAAGCAGAAAATATCATCAAAACCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGTGCTGCGCTTGAAGCCCTGCCCGGCGTAGGTCg                                                                    >  1:2399980/1‑150 (MQ=255)
                                                                                     aGGGGTGAAAACCTATATCAAAACGATTGGGCTTTATAACAGCAAAGCAGAAAATATCATCAAAACCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGTGCTGCGCTTGAAGCCCTGCCCGGCGTAGGTCGTaa                                                                 >  2:3035337/1‑150 (MQ=255)
                                                                                     aGGGGTGAAAACCTATATCAAAACGATTGGGCTTTATAACAGCAAAGCAGAAAATATCATCAAAACCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGTGCTGCGCTTGAAGCCCTGCCCGGCGTAGGTCGTaa                                                                 >  1:2196557/1‑150 (MQ=255)
                                                                                        ggTAAAACCCTATATAAAAACGATTGGGCTTTAAAACAGAAAAGCAAAAAATATCACCAAACCCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGTGCTGCGCTTGAAGCCCTGCCCGGCGTAGGTCGTAAAAc                                                              <  2:181003/150‑1 (MQ=255)
                                                                                         gtaaaacCCTATAACAAAACGTTTGGGTTTTAAAACAGCAAAGCAGAAAATATCATCAAACCCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGTGCTGCGCTTGAAGCCCTGCCCGGCGTAGGTCGTAAAACa                                                             <  2:2826839/147‑1 (MQ=255)
                                                                                         gTGAAAACCTATATCAAAACGATTGGGCTTTATAACAGCAAAGCAGAAAATATCATCAAAACCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGTGCTGCGCTTGAAGCCCTGCCCGGCGTAGGTCGTAAAACa                                                             >  1:2355742/1‑150 (MQ=255)
                                                                                           gAAACCCTATATCAAAACGATTGGGCTTTATAACAGAAAAGCAGAAAATATCATCAAACCCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGTGCTGCGCTTGAAGCCCTGCCCGGCGTAGGTCGTAAAACAGc                                                           <  1:2499374/150‑1 (MQ=255)
                                                                                            aaaaCCTATATCAAAACGATTGGGCTTTATAACAGCAAAGCAGAAAATATCATCAAAACCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGTGCTGCGCTTGAAGCCCTGCCCGGCGTAGGTCGTAAAACAGcc                                                          >  1:826227/1‑150 (MQ=255)
                                                                                              aaCCTATATCAAAACGATTGGGCTTTATAACAGCAAAGCAGAAAATATCATCAAAACCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGTGCTGCGCTTGAAGCCCTGCCCGGCGTAGGTCGTAAAACAGCCaa                                                        >  1:3183595/1‑150 (MQ=255)
                                                                                                ccTATATAAAAACGATGGGGTTTTATAACAGAAAAGAAAAAAATATCAAAAAACCCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGTGCTGCGCTTGAAGCCCTGCCCGGCGTAGGTCGTAAAACAGCCAAcg                                                      <  2:2298750/150‑1 (MQ=255)
                                                                                                 cgataaCAAAACGATTGGCCTTTATAACAGCAAAGCAGAAAATATCATCAAACCCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGTGCTGCGCTTGAAGCCCTGCCCGGCGTAGGTCGTAAAACAGCCAAcgt                                                     <  1:1100265/148‑1 (MQ=255)
                                                                                                 cTATATCAAAACGATTGGGCTTTATAACAGCAAAGCAGAAAATATCATCAAAACCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGTGCTGCGCTTGAAGCCCTGCCCGGCGTAGGTCGTAAAACAGCCAAcgt                                                     >  1:564017/1‑150 (MQ=255)
                                                                                                    tatCAAAACGATTGGGCTTTATAACAGCAAAGCAGAAAATATCATCAAAACCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGTGCTGCGCTTGAAGCCCTGCCCGGCGTAGGTCGTAAAACAGCCAAcgtcgt                                                  >  2:3502907/1‑150 (MQ=255)
                                                                                                       cAAAACGATTGGGCTTTATAACAGCAAAGCAGAAAATATCATCAAAACCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGTGCTGCGCTTGAAGCCCTGCCCGGCGTAGGTCGTAAAACAGCCAACGTCGTAtt                                               >  1:959626/1‑150 (MQ=255)
                                                                                                        aaaaCGATTGGGCTTTATAACAGCAAAGCAGAAAATATCATCAAAACCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGTGCTGCGCTTGAAGCCCTGCCCGGCGTAGGTCGTAAAACAGCCAACGTCGTATTa                                              >  1:3366588/1‑150 (MQ=255)
                                                                                                         aaaCGATTGGCCTTTATAACAGCAAAGCAGAAAATATCATCAAACCCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGTGCTGCGCTTGAAGCCCTGCCCGGCGTAGGTCGTAAAACAGCCAACGTCGTATTaa                                             <  1:3317971/150‑1 (MQ=255)
                                                                                                            cGATTGGGCTTTATAACAGCAAAGCAGAAAATATCATCAAAACCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGTGCTGCGCTTGAAGCCCTGCCCGGCGTAGGTCGTAAAACAGCCAACGTCGTATTAAaca                                          >  2:3291671/1‑150 (MQ=255)
                                                                                                              aTTGGGCTTTATAACAGCAAAGCAGAAAATATCATCAAAACCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGTGCTGCGCTTGAAGCCCTGCCCGGCGTAGGTCGTAAAACAGCCAACGTCGTATTAAACACt                                        >  2:3952741/1‑150 (MQ=255)
                                                                                                               ttGGGCTTTATAACAGCAAAGCAGAAAATATCATCAAAACCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGTGCTGCGCTTGAAGCCCTGCCCGGCGTAGGTCGTAAAACAGCCAACGTCGTATTAAACACTg                                       >  1:1298664/1‑150 (MQ=255)
                                                                                                                tGGGCTTTAAAACAGAAAAGAAAAAAATATCATAAAACCCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGTGCTGCGCTTGAAGCCCTGCCCGGCGTAGGTCGTAAAACAGCCAACGTCGTATTAAACACTGc                                      <  2:1832825/150‑1 (MQ=255)
                                                                                                                gggtttttAAAACAGAAAAGAAGAAAATATCACCAAAACCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGTGCTGCGCTTGAAGCCCTGCCCGGCGTAGGTCGTAAAACAGCCAACGTCGTATTAAACACTGc                                      <  2:3443464/145‑1 (MQ=255)
                                                                                                                   gCTTTATAACAGCAAAGCAGAAAATATCATCAAAACCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGTGCTGCGCTTGAAGCCCTGCCCGGCGTAGGTCGTAAAACAGCCAACGTCGTATTAAACACTGCAtt                                   >  1:3835113/1‑150 (MQ=255)
                                                                                                                   gCTTTAAAACAGCAAAGCAAAAAATATCATCAAACCCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGTGCTGCGCTTGAAGCCCTGCCCGGCGTAGGTCGTAAAACAGCCAACGTCGTATTAAACACTGCAtt                                   <  1:2585940/150‑1 (MQ=255)
                                                                                                                    cTTTATAACAGCAAAGCAGAAAATATCATCAAAACCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGTGCTGCGCTTGAAGCCCTGCCCGGCGTAGGTCGTAAAACAGCCAACGTCGTATTAAACACTGCATTc                                  >  2:211563/1‑150 (MQ=255)
                                                                                                                    cTTTAAAACAGCAAAGCAGAAAATATCATCAAACCCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGTGCTGCGCTTGAAGCCCTGCCCGGCGTAGGTCGTAAAACAGCCAACGTCGTATTAAACACTGCATTc                                  <  1:2164209/150‑1 (MQ=255)
                                                                                                                      ttATAACAGCAAAGCAGAAAATATCATAAAACCCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGTGCTGCGCTTGAAGCCCTGCCCGGCGTAGGTCGTAAAACAGCCAACGTCGTATTAAACACTGCATTCgg                                <  1:3063605/150‑1 (MQ=255)
                                                                                                                        aaaaCAGAAAAGAAAAAAATATCAACAAACCCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGTGCTGCGCTTGAAGCCCTGCCCGGCGTAGGTCGTAAAACAGCCAACGTCGTATTAAACACTGCATTCGGCt                              <  2:2437224/148‑1 (MQ=255)
                                                                                                                         taACAGCAAAGCAGAAAATATCATCAAACCCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGTGCTGCGCTTGAAGCCCTGCCCGGCGTAGGTCGTAAAACAGCCAACGTCGTATTAAACACTGCATTCGGCTg                             <  1:3873922/150‑1 (MQ=255)
                                                                                                                         taACAGCAAAGCAGAAAATATCATCAAAACCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGTGCTGCGCTTGAAGCCCTGCCCGGCGTAGGTCGTAAAACAGCCAACGTCGTATTAAACACTGCATTCGGCTg                             >  2:165166/1‑150 (MQ=255)
                                                                                                                         taACACAAAAGAAGAAAATATCATAAAAACCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGTGCTGCGCTTGAAGCCCTGCCCGGCGTAGGTCGTAAAACAGCCAACGTCGTATTAAACACTGCATTCGGCTg                             <  2:1365772/150‑1 (MQ=255)
                                                                                                                          aaCAGCAAAGCAGAAAATATCATCAAAACCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGTGCTGCGCTTGAAGCCCTGCCCGGCGTAGGTCGTAAAACAGCCAACGTCGTATTAAACACTGCATTCGGCTgg                            >  2:1335284/1‑150 (MQ=255)
                                                                                                                          aaCAGCAAAGCAGAAAATATCATCAAAACCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGTGCTGCGCTTGAAGCCCTGCCCGGCGTAGGTCGTAAAACAGCCAACGTCGTATTAAACACTGCATTCGGCTgg                            >  2:2254461/1‑150 (MQ=255)
                                                                                                                            cAGCAAAGCAGAAAATATCATCAAAACCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGTGCTGCGCTTGAAGCCCTGCCCGGCGTAGGTCGTAAAACAGCCAACGTCGTATTAAACACTGCATTCGGCTGGcc                          >  1:3510203/1‑150 (MQ=255)
                                                                                                                                aaaGCAGAAAATATCATCAAACCCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGTGCTGCGCTTGAAGCCCTGCCCGGCGTAGGTCGTAAAACAGCCAACGTCGTATTAAACACTGCATTCGGCTGGCCGACt                      <  1:2734080/150‑1 (MQ=255)
                                                                                                                                aaaGCAGAAAATATCATCAAAACCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGTGCTGCGCTTGAAGCCCTGCCCGGCGTAGGTCGTAAAACAGCCAACGTCGTATTAAACACTGCATTCGGCTGGCCGACt                      >  2:1277444/1‑150 (MQ=255)
                                                                                                                                aaaGCAGAAAATATCATCAAAACCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGTGCTGCGCTTGAAGCCCTGCCCGGCGTAGGTCGTAAAACAGCCAACGTCGTATTAAACACTGCATTCGGCTGGCCGACt                      >  1:139808/1‑150 (MQ=255)
                                                                                                                                 aaGCAGAAAATATCATCAAAACCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCCGAAGATCGTGCTACGCTTGAAGCCCTGCCCGGCGTAGGGCGTAAAACAGCCAACGGCGTATTAAACACTGCATTCGGCTGGCCGACTa                     >  2:3612255/1‑150 (MQ=255)
                                                                                                                                   gaaaaaaaTATCATAAAACCCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGTGCTGCGCTTGAAGCCCTGCCCGGCGTAGGTCGTAAAACAGCCAACGTCGTATTAAACACTGCATTCGGCTGGCCGACTAtt                   <  2:863882/146‑1 (MQ=255)
                                                                                                                                   gCAAAAAATATCATCAAACCCTGCCGTATCTTGCTGCAGCAGCATAATGGCGAGGTTCCGGAAGATCGTGCTGCGCTTGAAGCCCTGCCCGGCGTAGGTCGTAAAACAGCCAACGTCGTATTAAACACTGCATTCGGCTGGCCGACTAtt                   <  1:3376801/150‑1 (MQ=255)
                                                                                                                                        aaaTATCACCAAACCCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGTGCTGCGCTTGAAGCCCTGCCCGGCGTAGGTCGTAAAACAGCCAACGTCGTATTAAACACTGCATTCGGCTGGCCGACTATTGCTGt              <  2:326480/150‑1 (MQ=255)
                                                                                                                                         aaTATCATCAAAACCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGTGCTGCGCTTGAAGCCCTGCCCGGCGTAGGTCGTAAAACAGCCAACGTCGTATTAAACACTGCATTCGGCTGGCCGACTATTGCTGTc             >  2:3355402/1‑150 (MQ=255)
                                                                                                                                          atatCATCAAAACCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGTGCTGCGCTTGAAGCCCTGCCCGGCGTAGGTCGTAAAACAGCCAACGTCGTATTAAACACTGCATTCGGCTGGCCGACTATTGCTGTCg            >  1:2088701/1‑150 (MQ=255)
                                                                                                                                            atcatcAAAACCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGTGCTGCGCTTGAAGCCCTGCCCGGCGTAGGTCGTAAAACAGCCAACGTCGTATTAAACACTGCATTCGGCTGGCCGACTATTGCTGTCGac          >  1:2613738/1‑150 (MQ=255)
                                                                                                                                            atcatcAAAACCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGGTCCGGAAGATCGTGCTGCGCTTGAAGCCCTGCCCGGCGTAGGTCGTAAAACAGCCAACGTCGTATTAAACACTGCATTCGGCTGGCCGACTATTGCTGTCGac          >  1:703598/1‑150 (MQ=255)
                                                                                                                                              cataaAACCCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGTGCTGCGCTTGAAGCCCTGCCCGGCGTAGGTCGTAAAACAGCCAACGTCGTATTAAACACTGCATTCGGCTGGCCGACTATTGCTGTCGacac        <  1:1000732/150‑1 (MQ=255)
                                                                                                                                               atcaAACCCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGTGCTGCGCTTGAAGCCCTGCCCGGCGTAGGTCGTAAAACAGCCAACGTCGTATTAAACACTGCATTCGGCTGGCCGACTATTGCTGTCGACACg       <  1:3978801/150‑1 (MQ=255)
                                                                                                                                               atcaAAACCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGTGCTGCGCTTGAAGCCCTGCCCGGCGTAGGTCGTAAAACAGCCAACGTCGTATTAAACACTGCATTCGGCTGGCCGACTATTGCTGTCGACACg       >  1:1532963/1‑150 (MQ=255)
                                                                                                                                                  aaaaCCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGTGCTGCGCTTGAAGCCCTGCCCGGCGTAGGTCGTAAAACAGCCAACGTCGTATTAAACACTGCATTCGGCTGGCCGACTATTGCTGTCGACACGcac    >  1:1071075/1‑150 (MQ=255)
                                                                                                                                                  aaaaCCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGTGCTGCGCTTGAAGCCCTGCCCGGCGTAGGTCGTAAAACAGCCAACGTCGTATTAAACACTGCATTCGGCTGGCCGACTATTGCTGTCGACACGcac    >  1:2776195/1‑150 (MQ=255)
                                                                                                                                                    aaCCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGTGCTGCGCTTGAAGCCCTGCCCGGCGTAGGTCGTAAAACAGCCAACGTCGTATTAAACACTGCATTCGGCTGGCCGACTATTGCTGTCGACACGCACAt  >  1:1652515/1‑150 (MQ=255)
                                                                                                                                                    aaCCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGTGCTGCGCTTGAAGCCCTGCCCGGCGTAGGTCGTAAAACAGCCAACGTCGTATTAAACACTGCATTCGGCTGGCCGACTATTGCTGTCGACACGCACAt  >  1:762704/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
CGACCGATGTCAGTGTTAATAAGGCGACGGCGAAACTCTACCCGGTGGCGAATACGCCTGCAGCGATGCTTGAACTGGGCGTTGAAGGGGTGAAAACCTATATCAAAACGATTGGGCTTTATAACAGCAAAGCAGAAAATATCATCAAAACCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGTGCTGCGCTTGAAGCCCTGCCCGGCGTAGGTCGTAAAACAGCCAACGTCGTATTAAACACTGCATTCGGCTGGCCGACTATTGCTGTCGACACGCACAT  >  AP012306/1393230‑1393527

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑