Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP012306800,6750AC29.5% 577.2 / 40.4 268T192P (ACC→CCC) lolAchaperone for lipoproteins
Reads supporting (aligned to +/- strand):  ref base A (139/50);  new base C (0/79);  total (139/129)
Fisher's exact test for biased strand distribution p-value = 9.08e-34
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 323 total aligned reads displayed.

GCAATGGCAATCTGAAGCAGTTCACCATTAACGTGGGACGTGATGGCACAATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAAT‑GGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCAGAAAATTTAGCACAGTATATCGGCCAGCAACA  >  AP012306/800526‑800824
                                                                                                                                                      |                                                                                                                                                     
gCAATGGCAATCTGAAGCAGTTCACCATTAACGTGGGACGTGATGGCACAATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAAT‑GGGGCTGTGGATGCAGCGAAATTTACCTTCa                                                                                                                                                       >  2:1982687/1‑150 (MQ=255)
 cAATGGCAATCTGAAGCAGTTCACCATTAACGTGGGACGTGATGGCACAATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAAT‑GGGGCTGTGGATGCAGCGAAATTTACCTTCAc                                                                                                                                                      <  2:369416/150‑1 (MQ=255)
  aaTGGCAATCTGAAGCAGTTCACCATTAACGTGGGACGTGATGGCACAATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAAT‑GGGGCTGTGGATGCAGCGAAATTTACCTTCAcc                                                                                                                                                     <  2:505686/150‑1 (MQ=255)
  aaTGGCAATCTGAAGCAGTTCACCATTAACGTGGGACGTGATGGCACAATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAAT‑GGGGCTGTGGATGCAGCGAAATTTACCTTCAcc                                                                                                                                                     <  1:816600/150‑1 (MQ=255)
   aTGGCAATCTGAAGCAGTTCACCATTAACGTGGGACGTGATGGCACAATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAAT‑GGGGCTGTGGATGCAGCGAAATTTACCTTCAccc                                                                                                                                                    <  1:757255/150‑1 (MQ=255)
     ggCAATCTGAAGCAGTTCACCATTAACGTGGGACGTGATGGCACAATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAAT‑GGGGCTGTGGATGCAGCGAAATTTACCTTCACCccg                                                                                                                                                  >  2:1842378/1‑150 (MQ=255)
     ggCAATCTGAAGCAGTTCACCATTAACGTGGGACGTGATGGCACAATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAAT‑GGGGCTGTGGATGCAGCGAAATTTACCTTCACCccg                                                                                                                                                  >  1:1700477/1‑150 (MQ=255)
             gAAGCAGTTCACCATTAACGTGGGACGTGATGGCACAATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAACCCCAGAAAAAG‑GGGGCTGTGGATGCAGCGAAATTTCCCTTCCCCCCGCCGCAAgg                                                                                                                                          <  2:3945310/150‑1 (MQ=255)
               aGCAGTTCACCATTAACGTGGGACGTGATGGCACAATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAAT‑GGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCg                                                                                                                                        >  2:1209496/1‑150 (MQ=255)
                   gTTCACCATTAACGTGGGACGTGATGGCACAATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAAT‑GGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCAc                                                                                                                                    >  2:3131188/1‑150 (MQ=255)
                      cACCATTAACGTGGGACGTGATGGCACAATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAAT‑GGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGt                                                                                                                                 >  1:1297604/1‑150 (MQ=255)
                       aCCATTAACGTGGGACGTGATGGCACAATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAAT‑GGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTa                                                                                                                                >  1:348647/1‑150 (MQ=255)
                          aTTAACGTGGGACGTGATGGCACAATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGAAAAAT‑GGGGCTGTGGATGCAGCGAAATTTACCTTCCCCCCGCCGCAAGGCGTCACGGTAgat                                                                                                                             <  1:3748318/150‑1 (MQ=255)
                          aTTAACGGGGGACGTGATGGCAAAATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTAAAATCCCAGCAAAAG‑GGGGCTGGGGATGCAGCAAAATTTCCCTCCCCCCCGCCGCAAGGCGTCACGGTAgat                                                                                                                             <  1:2423430/150‑1 (MQ=255)
                             aaCGTGGGACGTGATGGCACAATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAAT‑GGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAgatgat                                                                                                                          >  2:2511546/1‑150 (MQ=255)
                              aggTGGGACGTGATGGCACAATCCATCAGTTTAGCGGGGGGGAGCAGGACGATCAGCGCAGCAGTTATCAACTAAAACCCCAACAAAAT‑GGGGCTGTGGATGCAGCGAAATTTCCCTCCCCCCCGCCGCAAGGCGTCACGGTAGATGATc                                                                                                                         <  2:1720692/148‑1 (MQ=255)
                              aCGTGGGACGTGATGGCACAATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAAT‑GGGGCTGTGGATGCAGCGAAATTTCCCTTCCCCCCGCCGCAAGGCGTCACGGTAGATGATc                                                                                                                         <  1:3372700/150‑1 (MQ=255)
                              aCGTGGGACGTGATGGCACAATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAAT‑GGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATc                                                                                                                         >  2:3838367/1‑150 (MQ=255)
                               cgggggACGTGATGGCACAATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTAAAACCCCAGAAAAAG‑GGGGCTGTGGATGCAGCGAAATTTCCCTCCCCCCCGCCGCAAGGCGTCACGGTAGATGATCa                                                                                                                        <  2:1914993/147‑1 (MQ=255)
                               cGTGGGACGTGATGGCACAATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTAAAACCCCAGAAAAAT‑GGGGCTGTGGATGCAGCAAAATTTACCTTCCCCCCGCCGCAAGGCGTCACGGTAGATGATCa                                                                                                                        <  1:4054008/150‑1 (MQ=255)
                                gTGGGACGTGATGGCACAATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAAG‑GGGGCTGTGGATGCAGCGAAATTTACCTTCCCCCCGCCGCAAGGCGTCACGGTAGATGATCaa                                                                                                                       <  1:2465096/150‑1 (MQ=255)
                                    gACGTGATGGCACAATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAAT‑GGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTa                                                                                                                   >  1:2955223/1‑150 (MQ=255)
                                    gACGTGATGGCACAATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAAT‑GGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTa                                                                                                                   >  1:2849506/1‑150 (MQ=255)
                                    gACGTGATGGCACAATCCATCAGTTTAGCGCGGGGGAGCAGGACGATCAGCGCAGCAGTTACCAACTAAAACCCCAGAAAAAG‑GGGGCTGTGGATGCAGCGAAATTTCCCTTCCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTa                                                                                                                   <  2:1757126/150‑1 (MQ=255)
                                      cGTGATGGCACAATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAAT‑GGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAg                                                                                                                 >  1:2040477/1‑150 (MQ=255)
                                       gTGATGGCACAATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAAT‑GGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGt                                                                                                                >  1:3921876/1‑150 (MQ=255)
                                          aTGGCACAATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAAT‑GGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTaga                                                                                                             >  1:1110223/1‑150 (MQ=255)
                                             gCACAATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGAAAAAT‑GGGGCTGTGGATGCAGCGAAATTTACCTTCCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGc                                                                                                          <  1:1076956/150‑1 (MQ=255)
                                                caATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAAT‑GGGGCTGTGGATGCAGCGAAATTTACCTCCCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCAcc                                                                                                       <  2:2331713/150‑1 (MQ=255)
                                                 aaTCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAAT‑GGGGCTGTGGATGCAGCGAAATTTCCCTCCCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCt                                                                                                      <  2:3592681/150‑1 (MQ=255)
                                                  aTCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAAT‑GGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCtg                                                                                                     >  1:2910593/1‑150 (MQ=255)
                                                    ccATCAGTGTGCCGGGGTGGAGAAGGACGACCAGCGCAGCATTTATAAACTAAAACCCCAGAAAAAG‑GGGGTTGTGGAGGCAGCAAAATTTCCCCTCCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCtgag                                                                                                   <  2:4051687/150‑1 (MQ=255)
                                                      aTCAGTTTAGCGCGGTGGAGAAGGACGATCAGCTCAGGATTTATCAACTGAAACCCCAGCAAAAG‑GGGGCTGTGAAGGCAGCAAAATTTCCCTTCCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCtgagtg                                                                                                 <  2:455465/150‑1 (MQ=255)
                                                       tCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGAAAAAT‑GGGGCTGTGGATGCAGCGAAATTTCCCTTCCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCtgagtga                                                                                                <  2:329822/150‑1 (MQ=255)
                                                        ctgTTTAGCGCGGGGGCGCAGGACGAGCGGCGCAGCAGTTATCACGGGAAACCCAAGAAAAAG‑GGGGTTGGGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCtgagtgag                                                                                               <  2:2801935/148‑1 (MQ=255)
                                                        cAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAAT‑GGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCtgagtgag                                                                                               >  2:3952947/1‑150 (MQ=255)
                                                         aGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAAT‑GGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGc                                                                                              >  1:3144949/1‑150 (MQ=255)
                                                          gTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAAT‑GGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCa                                                                                             >  1:1485911/1‑150 (MQ=255)
                                                          gTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAACCCCAGCAAAAT‑GGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCa                                                                                             <  1:895243/150‑1 (MQ=255)
                                                           tttAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTAAAATCCCAGCAAAAT‑GGGGCTGTGGATGCAGCGAAATTTCCCTTCCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCaa                                                                                            <  2:2123125/150‑1 (MQ=255)
                                                            ttAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAAT‑GGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAAt                                                                                           <  1:50801/150‑1 (MQ=255)
                                                             tAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAAT‑GGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATc                                                                                          <  1:2559897/150‑1 (MQ=255)
                                                                  cgGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAAT‑GGGGCTGTGGATGCAGCGAAATTTACCTTCCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCg                                                                                     <  1:3107637/150‑1 (MQ=255)
                                                                  cgGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAAT‑GGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCg                                                                                     >  2:3249731/1‑150 (MQ=255)
                                                                          aaGGACGATCAGCGCAGCATTTACAAACTAAAATCCCAGAAAAAT‑GGGGCTGTGGATGCAGCGAAATTTCCCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGAttt                                                                             <  2:263105/149‑1 (MQ=255)
                                                                           aGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAAT‑GGGGCTGTGGATGCAGCGAAATTTCCCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTCTCGCTCGAtttt                                                                            <  2:4007363/150‑1 (MQ=255)
                                                                           aGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAAT‑GGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGAtttt                                                                            >  2:13604/1‑150 (MQ=255)
                                                                             gACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAAT‑GGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTc                                                                          <  2:3417833/150‑1 (MQ=255)
                                                                               cGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAAT‑GGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCgg                                                                        >  2:2534138/1‑150 (MQ=255)
                                                                               cGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAAT‑GGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCgg                                                                        <  1:2324981/150‑1 (MQ=255)
                                                                                 aTCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAAT‑GGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGat                                                                      >  2:1281864/1‑150 (MQ=255)
                                                                                     gcgcAGCAGTTATCAACTGAAATCCCAGCAAAAT‑GGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGataata                                                                  >  2:1591720/1‑150 (MQ=255)
                                                                                        cagaagTTATCACCTGAAATCCCAGCAAAAT‑GGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACtt                                                               <  1:3843095/150‑1 (MQ=255)
                                                                                         agcagTTATCAACTGAAATCCCAGCAAAAT‑GGGGCTGTGGATGCAGCGAAATTTACCTTCCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACttt                                                              <  2:724151/150‑1 (MQ=255)
                                                                                         agcagTTATCAACTGAAATCCCAGCAAAAT‑GGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCGACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACttt                                                              >  1:1343508/1‑150 (MQ=255)
                                                                                         agcagTTATCAACTGAAATCCCAGCAAAAT‑GGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACttt                                                              >  2:920240/1‑150 (MQ=255)
                                                                                          gcagTTATCAACTGAAATCCCAGCAAAAT‑GGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACtttt                                                             <  1:186938/150‑1 (MQ=255)
                                                                                           cagTTATCAACTGAATTCCCAGCAAAAT‑GGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTc                                                            <  1:1359086/150‑1 (MQ=255)
                                                                                           cagTTATCAACTGAAATCCCAGCAAAAT‑GGGGCTGTGGATGCAGCGAAATTTCCCTTCCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTc                                                            <  2:2156135/150‑1 (MQ=255)
                                                                                            agTTATCAACTGAAATCCCAGCAAAAT‑GGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCa                                                           <  1:1002005/150‑1 (MQ=255)
                                                                                             gTTATCAACTGAAATCCCAGCAAAAT‑GGGGCTGTGGATGCAGCGAAATTTCCCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCaa                                                          <  1:2269722/150‑1 (MQ=255)
                                                                                              ttATCAACTGAAATCCCAGCAAAAT‑GGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAAc                                                         <  1:3972895/150‑1 (MQ=255)
                                                                                               tATCAACTGAAATCCCAGCAAAAT‑GGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAAcc                                                        >  2:3535011/1‑150 (MQ=255)
                                                                                                aTCAACTGAAACCCCAGCAAAAT‑GGGGCTGTGGATGCAGCGAAATTTCCCTTCCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACct                                                       <  2:814082/150‑1 (MQ=255)
                                                                                                  cAACTGAAATCCCAGCAAAAT‑GGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACctct                                                     >  2:2397623/1‑150 (MQ=255)
                                                                                                  cAACTGAAACTCCAGCAAAAT‑GGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACctct                                                     <  1:2924020/150‑1 (MQ=255)
                                                                                                    aCTGAAATCCCAGCAAAAT‑GGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTgg                                                   >  1:2745536/1‑150 (MQ=255)
                                                                                                    aCTGAAACCCCAGCAAAAT‑GGGGCTGTGGATGCAGCGAAATTTACCTTCCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTgg                                                   <  2:2764875/150‑1 (MQ=255)
                                                                                                       gAAATCCCAGCAAAAT‑GGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCcg                                                >  2:3866658/1‑150 (MQ=255)
                                                                                                       gAAATCCCAGCAAAAT‑GGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCcg                                                >  1:647885/1‑150 (MQ=255)
                                                                                                       gAAATCCCAGCAAAAT‑GGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCcg                                                <  1:3552988/150‑1 (MQ=255)
                                                                                                          accccAGAAAAAT‑GGGGCTGTGGATGCAGCGAAATTTACCTTCCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCcgcgc                                             <  2:1412053/148‑1 (MQ=255)
                                                                                                            cccAGCAAAAT‑GGGGTTGTGGATGGAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGt                                           <  1:60410/150‑1 (MQ=255)
                                                                                                                gaaaaaT‑GGGCAGGTGGATGACGGGAATTTCACCTTCGCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGc                                       <  1:1161470/148‑1 (MQ=255)
                                                                                                                gaaaaaG‑GGGGCTGGGGATGCAGCAAAATTTCCCTCCCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGc                                       <  2:4089866/148‑1 (MQ=255)
                                                                                                                 aaaaaTGGGGGTTGTGGAGGAGGCGAAATTTTCCTTCCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGGCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGc                                       <  2:3194465/149‑1 (MQ=255)
                                                                                                                  aaaaT‑GGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCgg                                     >  2:866835/1‑150 (MQ=255)
                                                                                                                    aaT‑GGGGCTGTGGATCCAGCGAAATTTACCTTCCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGcc                                   <  1:1384598/150‑1 (MQ=255)
                                                                                                                         gggCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCAGaa                               >  1:1020811/1‑150 (MQ=255)
                                                                                                                         gggCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCAGaa                               >  2:3635486/1‑150 (MQ=255)
                                                                                                                         gggCTGGGTATGCGGGAAATTTTTCCTCCCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCAGaa                               <  2:1536611/150‑1 (MQ=255)
                                                                                                                            ttgtgGATGCAGCAAAATTTCCCTTCCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCCCGCGTATGCGGCCAGAAAAt                            <  2:3918957/149‑1 (MQ=255)
                                                                                                                            cTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCAGAAAAt                            >  1:412982/1‑150 (MQ=255)
                                                                                                                            cTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCAGAAAAt                            <  1:2162822/150‑1 (MQ=255)
                                                                                                                             tgtgGATGCTGCAAATTTTACCTCCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCAGAAAAtt                           <  1:1911864/150‑1 (MQ=255)
                                                                                                                             tgtgGATGCAGCGAAATTTCCCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCAGAAAAtt                           <  1:946451/150‑1 (MQ=255)
                                                                                                                              gtgGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCAGAAAAttt                          <  2:1053684/150‑1 (MQ=255)
                                                                                                                                ggATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCAGAAAATTTAg                        >  2:49138/1‑150 (MQ=255)
                                                                                                                                     cAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCAGAAAATTTAGCACAg                   <  2:2687085/150‑1 (MQ=255)
                                                                                                                                      aGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCAGAAAATTTAGCACAGt                  >  1:2663090/1‑150 (MQ=255)
                                                                                                                                        cGAAATTTCCCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCAGAAAATTTAGCACAGtat                <  2:2856821/150‑1 (MQ=255)
                                                                                                                                        cGAAATTTACCTTCCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCAGAAAATTTAGCACAGtat                <  1:3241966/150‑1 (MQ=255)
                                                                                                                                         gAAATTTACCTTCCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCAGAAAATTTAGCACAGtata               <  2:2932618/150‑1 (MQ=255)
                                                                                                                                          aaaTTTACCTTCCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCAGAAAATTTAGCACAGTatat              <  2:1116350/150‑1 (MQ=255)
                                                                                                                                           aaTTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCAGAAAATTTAGCACAGTATATc             >  2:3788895/1‑150 (MQ=255)
                                                                                                                                           aaTTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCAGAAAATTTAGCACAGTATATc             >  2:754653/1‑150 (MQ=255)
                                                                                                                                            aTTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCAGAAAATTTAGCACAGTATATCg            >  1:3657646/1‑150 (MQ=255)
                                                                                                                                              ttACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCAGAAAATTTAGCACAGTATATCGGc          <  1:238032/150‑1 (MQ=255)
                                                                                                                                               tACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCAGAAAATTTAGCACAGTATATCGGcc         >  2:1461053/1‑150 (MQ=255)
                                                                                                                                                      aCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCAGAAAATTTAGCACAGTATATCGGCCAGCAACa  >  1:580092/1‑150 (MQ=255)
                                                                                                                                                      |                                                                                                                                                     
GCAATGGCAATCTGAAGCAGTTCACCATTAACGTGGGACGTGATGGCACAATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAAT‑GGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCAGAAAATTTAGCACAGTATATCGGCCAGCAACA  >  AP012306/800526‑800824

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑