Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP012306935,0540GT25.0% 273.3 / 42.9 96intergenic (+24/‑1406)putP/ycdOproline:sodium symporter/conserved protein
Reads supporting (aligned to +/- strand):  ref base G (16/56);  new base T (24/0);  total (40/56)
Fisher's exact test for biased strand distribution p-value = 2.41e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 172 total aligned reads displayed.

GGCAGTATTGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTG‑TTTGGCTT‑CAGCAGCGGGTTGCG‑CT‑CCCTTAATGTG‑CCTCGCCATATAAATTGAATGGTGCAGGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCC‑TTCGGGGAACGCTTCAGCGATTTTGACGCCACCAACA  >  AP012306/934905‑935203
                                                                                                                                                      |                                                                                                                                                           
 gCAGTATTGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTtttg‑t                                                                                                                                                           >  1:2577923/1‑150 (MQ=255)
 gCAGTATTGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTtttg‑t                                                                                                                                                           <  1:2000630/150‑1 (MQ=255)
  cAGTATTGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGGtttt‑tt                                                                                                                                                          >  2:412691/1‑150 (MQ=255)
   aGTATTGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGgtttg‑ttt                                                                                                                                                         >  2:1083090/1‑150 (MQ=255)
   aGTATTGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGCtttg‑ttt                                                                                                                                                         >  2:193268/1‑150 (MQ=255)
    gTATTGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGttttt‑tttt                                                                                                                                                        >  1:3508682/1‑149 (MQ=255)
    gTATTGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGttttt‑tttt                                                                                                                                                        >  2:48766/1‑149 (MQ=255)
    gTATTGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTtttt‑tttg                                                                                                                                                        >  2:2497688/1‑150 (MQ=255)
     tATTGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGttttt‑ttttg                                                                                                                                                       >  2:1651373/1‑148 (MQ=255)
     tATTGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTtttg‑ttttg                                                                                                                                                       >  1:4031278/1‑148 (MQ=255)
     tATTGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTG‑TTTgg                                                                                                                                                       <  1:3664377/150‑1 (MQ=255)
     tATTGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGGtttt‑ttttg                                                                                                                                                       >  2:1034627/1‑148 (MQ=255)
      aTTGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGttttt‑tttttc                                                                                                                                                      >  1:3628786/1‑147 (MQ=255)
      aTTGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGCTTTG‑TTTTGc                                                                                                                                                      >  2:2106240/1‑150 (MQ=255)
       ttGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGGTTTTT‑TTTggt                                                                                                                                                      >  2:2060121/1‑149 (MQ=255)
        tGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTATTT‑TTTGGCtt                                                                                                                                                    >  1:1458525/1‑150 (MQ=255)
         gggATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGGTTTTT‑TTTGGttt                                                                                                                                                    >  1:2960287/1‑150 (MQ=255)
         gggATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGCtttt‑ttttgtctc                                                                                                                                                   >  2:3325597/1‑144 (MQ=255)
          ggATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGGtttt‑ttttgttttt                                                                                                                                                  >  2:291192/1‑143 (MQ=255)
           gATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTG‑TTTGGCTT‑cag                                                                                                                                                <  2:3292187/150‑1 (MQ=255)
            aTTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGGTTTTT‑TTTGGCttagc                                                                                                                                                 >  1:3205235/1‑147 (MQ=255)
            aTTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTT‑TTTTGCTT‑cagc                                                                                                                                               >  1:710237/1‑150 (MQ=255)
                tAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTT‑TTTTGCTT‑TCGCAGCg                                                                                                                                           >  2:3733902/1‑150 (MQ=255)
                 aGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTG‑TTTGGCTT‑CAGCAGCgg                                                                                                                                          >  1:1601791/1‑150 (MQ=255)
                    gtTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTT‑TTTTGCTT‑CAGCAGCggggt                                                                                                                                       >  1:2852583/1‑148 (MQ=255)
                    gtTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTT‑TTTTGCGT‑CAGCACCgccgt                                                                                                                                       >  2:604632/1‑146 (MQ=255)
                    gtTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGGTTTT‑TTTTGCTT‑CAGCAACggggt                                                                                                                                       >  2:40566/1‑148 (MQ=255)
                      ttAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTG‑TTTTGGTT‑TAGCAGCGGGGTgg                                                                                                                                     >  2:2181491/1‑149 (MQ=255)
                       tAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTG‑TTTTGCTT‑CAGCAGCGGGGTgcg                                                                                                                                    >  1:1287662/1‑150 (MQ=255)
                         gTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTG‑TTTGGCTT‑CAGCAGCGGGTTGCG‑Ct                                                                                                                                 <  1:41942/150‑1 (MQ=255)
                          tttGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTGTTTTGGCTT‑C‑TCCGCGGGTTGCG‑CT‑c                                                                                                                               >  1:1026072/1‑150 (MQ=255)
                          tttGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTG‑TTTGGCTT‑CAGCAGCGGGTTGCG‑CT‑c                                                                                                                               <  1:3839767/150‑1 (MQ=255)
                           ttGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTG‑TTTGGCTT‑CAGCAGCGGGTTGCG‑CT‑cc                                                                                                                              <  2:2272850/150‑1 (MQ=255)
                             gCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTG‑TTTGGCTT‑CAGCAGCGGGTTGCG‑CT‑CCCt                                                                                                                            <  1:2214996/150‑1 (MQ=255)
                             gCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTG‑TTTGGCTT‑CAGCAGCGGGTTGCG‑CT‑CCCt                                                                                                                            <  2:2616633/150‑1 (MQ=255)
                              cTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGGTTTT‑TTTTGGTT‑CAGCAGCCGGGTGGG‑CT‑cccct                                                                                                                           >  2:3978217/1‑148 (MQ=255)
                                 ggTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTT‑TTTTGGTT‑CAGCAGCCGGGTGCG‑Cc‑ccccttat                                                                                                                        >  1:3981287/1‑145 (MQ=255)
                                 ggTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTT‑TTTTGCTT‑CACCCGCGGGCTTCG‑CC‑CCCTTTAt                                                                                                                        >  1:1566308/1‑150 (MQ=255)
                                 ggTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTT‑TTTGGATT‑TAACAGCGGGCGGCG‑CG‑CCCCTaaa                                                                                                                        >  2:2574725/1‑149 (MQ=255)
                                    aaaGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTG‑TTTGGCTT‑CAGCAGCGGGTTGCG‑CT‑CCCTTAAtgtg                                                                                                                     <  1:1385932/150‑1 (MQ=255)
                                     aaGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGGTTTT‑TTTGGGTT‑TCGCAGAGGGGTGGGCCC‑CCCTTAAttgg                                                                                                                     >  2:2598258/1‑147 (MQ=255)
                                       gcgcCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTT‑TTTTGCTT‑CCGCAGCGGGGTTCG‑CT‑CCCCTTATGTG‑GCt                                                                                                                 >  1:3257896/1‑150 (MQ=255)
                                         gcCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGGTTTT‑TTTGGGTT‑CAGCAGCGGGTTGTG‑CT‑CCCCTTATTTG‑GCTCg                                                                                                               >  2:3787780/1‑150 (MQ=255)
                                           cGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTG‑TTTGGCTT‑CAGCAGCGGGTTGCG‑CT‑CCCTTAATGTG‑CCTCGcc                                                                                                             <  1:2442497/150‑1 (MQ=255)
                                            gTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTT‑TTTTGCTT‑CAGCAGCGGGGTGCG‑CG‑CCCCTTATTTG‑GCTCGccc                                                                                                            >  1:2640968/1‑149 (MQ=255)
                                              cAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGTGACTTAGCCTGCGGC‑GGTTTTT‑TTTTGGTT‑TCGCAGCGGGGTGCG‑CT‑CCCCTTATTTG‑GCTCGCCcta                                                                                                          >  1:2940463/1‑150 (MQ=255)
                                               aGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTT‑TTTTGGTT‑TAGCAGCGGGGTGCG‑CTCCCCTTAAT‑TG‑CCCCGccccat                                                                                                         >  2:1071983/1‑146 (MQ=255)
                                               aGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTG‑TTTGGCTT‑CAGCAGCGGGTTGCG‑CT‑CCCTTAATGTG‑CCTCGCCatat                                                                                                         <  1:2433291/150‑1 (MQ=255)
                                               aGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTG‑TTTGGCTT‑CAGCAGCGGGTTGCG‑CT‑CCCTTAATGTG‑CCTCGCCatat                                                                                                         <  2:2193675/150‑1 (MQ=255)
                                               aGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGGTTTG‑TTTTTGTT‑TCGCAGGGGGGTGGG‑CC‑CCCCTTATGTG‑CCTCGCCctat                                                                                                         >  2:3712313/1‑150 (MQ=255)
                                                 cggcgATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTG‑TTTGGCTT‑CAGCAGCGGGTTGCG‑CT‑CCCTTAATGTG‑CCTCGCCATATaa                                                                                                       <  1:677925/150‑1 (MQ=255)
                                                 cggcgATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTG‑TTTGGCTT‑CAGCAGCGGGTTGCG‑CT‑CCCTTAATGTG‑CCTCGCCATATaa                                                                                                       <  1:3934175/150‑1 (MQ=255)
                                                  ggcgATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTT‑TTTTGCTT‑CAGCAGCGGGGTTCG‑CT‑CCCCTTATTTG‑CCTCGCCCTATaaa                                                                                                      >  1:619711/1‑150 (MQ=255)
                                                  ggcgATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGGTTTT‑TTTTGGTT‑TAGCAGCGGGGGGCG‑CC‑CCCCTTATTTG‑GCTCGCCCTATaaa                                                                                                      >  2:1473403/1‑150 (MQ=255)
                                                  ggcgATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGGTTTT‑TTTTGGTT‑CAGCCGCGGGGTGCG‑CT‑CCCCTTATGTG‑GCTCCCCCTCTaaa                                                                                                      >  2:2833863/1‑150 (MQ=255)
                                                   gcgATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTG‑TTTGGCTT‑CAGCAGCGGGTTGCG‑CT‑CCCTTAATGTG‑CCTCGCCATATAAAt                                                                                                     <  1:326315/150‑1 (MQ=255)
                                                   gcgATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGGTTTT‑TTTTGGTT‑CTGCAGGGGGGTGGG‑CT‑CCCCTTATTTG‑CCTCGCCCTCTaaaa                                                                                                     >  2:1654521/1‑149 (MQ=255)
                                                    cgATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTG‑TTTGGCTT‑CAGCAGCGGGTTGCG‑CT‑CCCTTAATGTG‑CCTCGCCATATAAAtt                                                                                                    <  1:358697/150‑1 (MQ=255)
                                                    cgATGCAAAAACGCTTTGCCGAGACCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTG‑TTTGGCTT‑CAGCAGCGGGTTGCG‑CT‑CCCTTAATGTG‑CCTCGCCATATAAAtt                                                                                                    <  2:3494676/150‑1 (MQ=255)
                                                     gATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGGTTTG‑TTTGGGTT‑TCGCCGCGGGGTGCG‑CT‑CCCCTTAAGTG‑CCTCGCCCTATAAAtta                                                                                                   >  2:2421549/1‑149 (MQ=255)
                                                      aTGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTG‑TTTGGCTT‑CAGCAGCGGGGTGCG‑CT‑CCCCTTATGTG‑CCTCGCCCTATAAAATGa                                                                                                  >  1:556516/1‑150 (MQ=255)
                                                      aTGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGGTTTT‑TTTTGTCT‑CATCCGGCGGTTTTG‑CC‑CCCCTTATTTGCCCTCCCCATTTAAATTg                                                                                                   >  2:2685781/1‑150 (MQ=255)
                                                           aaaaCGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTG‑TTTGGCTT‑CAGCAGCGGGTTGCG‑CT‑CCCTTAATGTG‑CCTCGCCATATAAATTGAATGGt                                                                                             <  1:2713639/150‑1 (MQ=255)
                                                                gCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTG‑TTTGGCTT‑CAGCAGCGGGTTGCG‑CT‑CCCTTAATGTG‑CCTCGCCATATAAATTGAATGGTGCAgg                                                                                        <  1:1382175/150‑1 (MQ=255)
                                                                 cTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTT‑TTTTGGTT‑TCGCAGCGGGGTGCG‑CT‑CCCCTTATTTG‑GCCCGCCCTATTAATTTAATTGGGGAggg                                                                                       >  2:832060/1‑150 (MQ=255)
                                                                 cTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTT‑TTTTGGTT‑CCCGAGCCGGTTCGT‑CT‑CCCTTTTTGTT‑TCTTGCCCTAAAAATTTAATggggttggg                                                                                       >  2:3249297/1‑143 (MQ=255)
                                                                     gCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTT‑TTTTGGTT‑CAGCAACGGGGTGCG‑CT‑CCCCTTATGTG‑CCCCGCCCTATAAAATTAAAGGTGCAGGGGgcg                                                                                   >  2:149963/1‑150 (MQ=255)
                                                                      ccGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTG‑TTTGGCTT‑CAGCAGCGGGTTGCG‑CT‑CCCTTAATGTG‑CCTCGCCATATAAATTGAATGGTGCAGGGAgcgc                                                                                  <  2:1485903/150‑1 (MQ=255)
                                                                        gAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGGTTTT‑TTTTGGTT‑TTGCAGCGGGGTGGG‑CT‑CCCCTTATGTG‑GCTCGCCCTATAAATTGAATGGTGGCGGGGgggcgc                                                                                >  2:3309841/1‑150 (MQ=255)
                                                                            ccGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTG‑TTTGGCTT‑CAGCAGCGGGTTGCG‑CT‑CCCTTAATGTG‑CCTCGCCATATAAATTGAATGGTGCAGGGAGCGCGCAggg                                                                            <  1:1950022/150‑1 (MQ=255)
                                                                             cGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTT‑TTTTTCTT‑CCACATCCGGGTGGG‑CT‑CCCCTATTTTGTCCTCCCCAAATAAATT‑TATGGGGGAAGGAGCGCGCAcggg                                                                           >  2:2860896/1‑150 (MQ=255)
                                                                               aTGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTG‑TTTGGCTT‑CAGCAGCGGGTTGCG‑CT‑CCCTTAATGTG‑CCTCGCCATATAAATTGAATGGTGCAGGGAGCGCGCAGGGggc                                                                         <  1:1737794/150‑1 (MQ=255)
                                                                                   gcACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTT‑TTTTGGTT‑TAGCAGCGGGGTGCG‑GC‑CCCCTTATTTG‑CCCCGCCCTTTTAATTTAATGGGGCCGGGGGGGCGCCgggggggggc                                                                     >  2:2401099/1‑148 (MQ=255)
                                                                                     aCTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGCTTTT‑TTTTGCTTCCACCACCGGGTGGCG‑C‑‑CCCTCTATGGGCCCTCCCCTTAAAAATTTATAGGGCCGGGGGGCGGGCGGGGGGGGGCCa                                                                    >  2:1431633/1‑150 (MQ=255)
                                                                                       tATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTG‑TTTGGCTT‑CAGCAGCGGGTTGCG‑CT‑CCCTTAATGTG‑CCTCGCCATATAAATTGAATGGTGCAGGGAGCGCGCAGGGGGCGGCCAATc                                                                 <  1:3765959/150‑1 (MQ=255)
                                                                                       tATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGGTTTT‑TTTTGCTT‑CCGCCGCGGGCTGTC‑CC‑CCCCTAATGTT‑GCTCCGCATAATAATTTAATGGTGCAGGGGGCGCGCAGGGGGCGGGCAATc                                                                 >  2:2246163/1‑150 (MQ=255)
                                                                                        aTCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGCTTTG‑TTTTGGTT‑CAGCAGCGGGGTGCG‑CT‑CCCTTAATGTG‑CCTCGCCCTTTAAATTGAATGGTGTTGGGAGCGCGGGGCGGGCGGCCAACcg                                                                >  2:698428/1‑150 (MQ=255)
                                                                                               ggCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGGTTTT‑TTTGGCTT‑CAGCAGCGGGGTGCG‑CT‑CCCCTTATGTG‑CCTCCCCCTATAAATTTGATTGTGCGGGGGGGGCGCGGGGGGCGGCCAATccccccccc                                                         >  2:185754/1‑148 (MQ=255)
                                                                                                    cgccgTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTT‑TTTTGCTT‑CCGCCGCGGGGTGCG‑CT‑CCCCTTATGTG‑CCTCCCCCTATAAAATTAATGGGGGCGGGGGCGCGCCGGGGGCGGCCCATCGCccccccccccc                                                    >  2:3532607/1‑149 (MQ=255)
                                                                                                       cgTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTG‑TTTTGGTT‑CAGCAGCGGGGTGCG‑CTCCCCTTAAT‑TG‑GCCCGCCCTATAAATTGGAAGGGGGGGGGGGCGCGCCGGGGGCGGCCCATCGCccccccccccccct                                                 >  2:3252341/1‑146 (MQ=255)
                                                                                                            cGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTT‑TTTTGGTT‑CAGCAGCGGGGTTCG‑CC‑CCCCTAATTTT‑CCCCGCCCTAAAAATTTGAAGGTGCAGGGGGGGCGCCGGGGGGGGGCCAACCCCGCCCCCCCCCTCTGTccc                                            >  2:3110417/1‑150 (MQ=255)
                                                                                                            cGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTT‑TTTTGCTT‑CAGCAGCGGGGTGGG‑CT‑CCCCTAATGTG‑CCTCGCCCTATAAAATTAATTGTGCAGGGGGCGCGCCGGGGGCGGGCCATCACCGCCCCCCCCCCCTTTccc                                            >  2:578509/1‑150 (MQ=255)
                                                                                                                tGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTG‑TTTGGCTT‑CAGCAGCGGGTTGCG‑CT‑CCCTTAATGTG‑CCTCGCCATATAAATTGAATGGTGCAGGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGcc                                        <  1:3210694/150‑1 (MQ=255)
                                                                                                                  cAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTG‑TTTGGCTT‑CAGCAGCGGGTTGCG‑CT‑CCCTTAATGTG‑CCTCGCCATATAAATTGAATGGTGCAGGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCC‑tt                                     <  2:129534/150‑1 (MQ=255)
                                                                                                                     gAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTG‑TTTGGCTT‑CAGCAGCGGGTTGCG‑CT‑CCCTTAATGTG‑CCTCGCCATATAAATTGAATGGTGCAGGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCC‑TTCgg                                  <  1:390277/150‑1 (MQ=255)
                                                                                                                        aGCTAAGGGACTTAGCCTGCGGC‑GGTTTTG‑TTTGGCTT‑CAGCAGCGGGTTGCG‑CT‑CCCTTAATGTG‑CCTCGCCATATAAATTGAATGGTGCAGGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCC‑TTCGGGGa                               <  1:910659/150‑1 (MQ=255)
                                                                                                                        aGCTAAGGGACTTAGCCTGCGGC‑GGTTTTG‑TTTGGCTT‑CAGCAGCGGGTTGCG‑CT‑CCCTTAATGTG‑CCTCGCCATATAAATTGAATGGTGCAGGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCC‑TTCGGGGa                               <  1:2316116/150‑1 (MQ=255)
                                                                                                                           tAAGGGACTTAGCCTGCGGC‑GGTTTTT‑TTTGGCTT‑CAGCCGCGGGGTGTG‑CC‑CCCCTAATGTG‑CCCCGCCCTTTTAATTTTATGGGGGCGGGGGCGCGCAGGGGGGGGGCCAACCCCCCCCCCCCCCCCTCTCCCCGCCTTTCGGGGacg                             >  2:1115895/1‑148 (MQ=255)
                                                                                                                                  cTTAGCCTGCGGC‑GGTTTTG‑TTTGGCTT‑CAGCAGCGGGTTGCG‑CT‑CCCTTAATGTG‑CCTCGCCATATAAATTGAATGGTGCAGGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCC‑TTCGGGGAACGCTTCAGc                     <  1:2088421/150‑1 (MQ=255)
                                                                                                                                   ttAGCCTGCGGC‑GGTTTTG‑TTTGGCTT‑CAGCAGCGGGTTGCG‑CT‑CCCTTAATGTG‑CCTCGCCATATAAATTGAATGGTGCAGGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCC‑TTCGGGGAACGCTTCAGCg                    <  1:2229677/150‑1 (MQ=255)
                                                                                                                                       ccTGCGGC‑GGTTTTG‑TTTGGCTT‑CAGCAGCGGGTTGCG‑CT‑CCCTTAATGTG‑CCTCGCCATATAAATTGAATGGTGCAGGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCC‑TTCGGGGAACGCTTCAGCGAttt                <  2:1308889/150‑1 (MQ=255)
                                                                                                                                         tGCGGC‑GGTTTTG‑TTTGGCTT‑CAGCAGCGGGTTGCG‑CT‑CCCTTAATGTG‑CCTCGCCATATAAATTGAATGGTGCAGGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCC‑TTCGGGGAACGCTTCAGCGATTTTg              <  1:202376/150‑1 (MQ=255)
                                                                                                                                          gcggc‑gGTTTTG‑TTTGGCTT‑CAGCAGCGGGTTGCG‑CT‑CCCTTAATGTG‑CCTCGCCATATAAATTGAATGGTGCAGGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCC‑TTCGGGGAACGCTTCAGCGATTTTGa             <  1:1901666/150‑1 (MQ=255)
                                                                                                                                                ggTTTTG‑TTTGGCTT‑CAGCAGCGGGTTGCG‑CT‑CCCTTAATGTG‑CCTCGCCATATAAATTGAATGGTGCAGGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCC‑TTCGGGGAACGCTTCAGCGATTTTGACGcca        >  2:1748048/1‑150 (MQ=255)
                                                                                                                                                ggTTTTG‑TTTGGCTT‑CAGCAGCGGGTTGCG‑CT‑CCCTTAATGTG‑CCTCGCCATATAAATTGAATGGTGCAGGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCC‑TTCGGGGAACGCTTCAGCGATTTTGACGcca        >  1:2874932/1‑150 (MQ=255)
                                                                                                                                                ggTTTTG‑TTTGGCTT‑CAGCAGCGGGTTGCG‑CT‑CCCTTAATGTG‑CCTCGCCATATAAATTGAATGGTGCAGGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCC‑TTCGGGGAACGCTTCAGCGATTTTGACGcca        >  1:3644005/1‑150 (MQ=255)
                                                                                                                                                ggTTTTG‑TTTGGCTT‑CAGCAGCGGGTTGCG‑CT‑CCCTTAATGTG‑CCTCGCCATATAAATTGAATGGTGCAGGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCC‑TTCGGGGAACGCTTCAGCGATTTTGACGcca        >  2:1342598/1‑150 (MQ=255)
                                                                                                                                                 gTTTTG‑TTTGGCTT‑CAGCAGCGGGTTGCG‑CT‑CCCTTAATGTG‑CCTCGCCATATAAATTGAATGGTGCAGGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCC‑TTCGGGGAACGCTTCAGCGATTTTGACGccac       >  1:286810/1‑150 (MQ=255)
                                                                                                                                                 gTTTTG‑TTTGGCTT‑CAGCAGCGGGTTGCG‑CT‑CCCTTAATGTG‑CCTCGCCATATAAATTGAATGGTGCAGGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCC‑TTCGGGGAACGCTTCAGCGATTTTGACGccac       <  1:1057597/150‑1 (MQ=255)
                                                                                                                                                  ttttG‑TTTGGCTT‑CAGCAGCGGGTTGCG‑CT‑CCCTTAATGTG‑CCTCGCCATATAAATTGAATGGTGCAGGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCC‑TTCGGGGAACGCTTCAGCGATTTTGACGccacc      >  1:3574287/1‑150 (MQ=255)
                                                                                                                                                      |                                                                                                                                                           
GGCAGTATTGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTG‑TTTGGCTT‑CAGCAGCGGGTTGCG‑CT‑CCCTTAATGTG‑CCTCGCCATATAAATTGAATGGTGCAGGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCC‑TTCGGGGAACGCTTCAGCGATTTTGACGCCACCAACA  >  AP012306/934905‑935203

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑