Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123061,944,2880AC36.8% 387.9 / 45.7 212G361G (GGT→GGG) menEo‑succinylbenzoate‑CoA ligase
Reads supporting (aligned to +/- strand):  ref base A (94/40);  new base C (0/78);  total (94/118)
Fisher's exact test for biased strand distribution p-value = 2.67e-28
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 255 total aligned reads displayed.

ACCCATTCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGC‑AATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCTTCGTCATTAACCAGTGAAACCAGTTGCCCGTTACGCCAGTAACCTTCTGCCAT  >  AP012306/1944139‑1944437
                                                                                                                                                      |                                                                                                                                                     
 cccATTCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGC‑AATTACGCGCTCGACTTCTTCCGGCTGAATAc                                                                                                                                                      <  2:2070317/150‑1 (MQ=255)
 cccATTCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGC‑AATTACGCGCTCGACTTCTTCCGGCTGAATAc                                                                                                                                                      >  1:2896641/1‑150 (MQ=255)
  ccATTCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGC‑AATTACGCGCTCGACTTCTTCCGGCTGAATAcc                                                                                                                                                     <  1:2284633/150‑1 (MQ=255)
     ttCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGC‑AATTACGCGCTCGACTTCTTCCGGCTGAATACCctc                                                                                                                                                  <  1:3607305/150‑1 (MQ=255)
     ttCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGC‑AATTACGCGCTCGACTTCTTCCGGCTGAATACCctc                                                                                                                                                  <  1:3352940/150‑1 (MQ=255)
      tCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGC‑AATTACGCGCTCGACTTCTTCCGGCTGAATACCctct                                                                                                                                                 <  1:128075/150‑1 (MQ=255)
      tCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGC‑AATTACGCGCTCGACTTCTTCCGGCTGAATACCctct                                                                                                                                                 <  2:2410858/150‑1 (MQ=255)
       cACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGC‑AATTACGCGCTCGACTTCTTCCGGCTGAATACCCtctc                                                                                                                                                >  2:211319/1‑150 (MQ=255)
         cTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGC‑AATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCg                                                                                                                                              >  1:648213/1‑150 (MQ=255)
         cTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGC‑AATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCg                                                                                                                                              >  2:1987420/1‑150 (MQ=255)
            aGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGC‑AATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCa                                                                                                                                           <  2:298649/150‑1 (MQ=255)
            aGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGC‑AATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCa                                                                                                                                           <  1:1747763/150‑1 (MQ=255)
             gaacaccGCCCTCGTGGTCATCCTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAACCCGCAGGATGTGCAGC‑AATTCCGCGCTCGACTTCTCCCGGCTGAATCCCCTCTCCGCCAc                                                                                                                                          <  2:383716/144‑1 (MQ=255)
              aTCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGC‑AATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACt                                                                                                                                         >  2:4079057/1‑150 (MQ=255)
                  aCGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGC‑AATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTgaag                                                                                                                                     >  2:2586216/1‑150 (MQ=255)
                     ctctCGTGGTCATACTCCATCCCCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAACCCGCAGGATGTGCAGC‑AATTACGCGCTCGACTTCTTCCGGCTGAATCCCCTCTCCGCCACTGAAGAAt                                                                                                                                  <  2:490925/150‑1 (MQ=255)
                      ttcggTGGTCATCCTCCACCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGC‑AATTCCGCGCCCGCCTTCTCCCGGCTGAATCCCCTCTCCGCCACTGAAGAATa                                                                                                                                 <  2:1570181/146‑1 (MQ=255)
                       ctcGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGC‑AATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTGAAGAATAg                                                                                                                                <  1:2449091/150‑1 (MQ=255)
                        tcGTGGTCATCCTCCATCACCGCCACCGGTCGATGACCAACCTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGC‑AATTACGCGCTCGACTTCTTCCGGCTGAATCCCCTCTCCGCCACTGAAGAATAGa                                                                                                                               <  1:267474/150‑1 (MQ=255)
                            ggTCTTACCCCACCACCGCCCCGGGTCGATGCCAAAACTCCTTCTCGGCAACGGGGACGATAAACCCCGGAAAAACCGCAGGATG‑GCAACAAATTACGCGTTCGATTTCTCCCGGCTGAATCCCCTTTCCGCCACTGAAGAATAGATTGt                                                                                                                           <  2:794992/150‑1 (MQ=255)
                            ggTCATCCTCCATCCCCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAACCCCTGCAAACCCGCAGGATGTGCAGC‑AATTACGCGCTCGACTTCTTCCGGCTGAATCCCCTCTCCGCCACTGAAGAATAGATTGt                                                                                                                           <  2:332738/150‑1 (MQ=255)
                               cATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGC‑AATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTGAAGAATAGATTGTCTa                                                                                                                        >  1:3429449/1‑150 (MQ=255)
                                  aCTCCATCCCCGCCACCGGCCGATGACAAACCTCCTTGTCGGCAACGGGGACGATAAACCCCTGCAAAACCGCAGGATGTGCTGC‑AATTACGCGCTCGACTTCTTCCGGCTGAATCCCCTCTCCGCCACTGAAGAATAGATTGTCTAAAc                                                                                                                     <  1:193957/150‑1 (MQ=255)
                                   cTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGC‑AATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTGAAGAATAGATTGTCTAAACg                                                                                                                    >  2:1224713/1‑150 (MQ=255)
                                      cATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGC‑AATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTcc                                                                                                                 >  1:2914496/1‑150 (MQ=255)
                                        tCACCGCCACGGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAACCCGCAGGATGTGCAGC‑AATTACGCGCTCGACTTCTCCCGGCTGAATCCCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGa                                                                                                               <  1:924375/150‑1 (MQ=255)
                                         ccccGCCACCGGTCGATGACCAAACCCCTTGTCGGCAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGC‑AATTACGCGCTCGATTTCTCCCGGCGGAATCCCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGAc                                                                                                              <  1:3086964/148‑1 (MQ=255)
                                          aCCGCCACCGGTCGATGACCAACCTCCTTGTCGGCAACGGGGACGATAAACCCCTGCAAAACCGCAGGATGTGCAGC‑AATTACGCGCTCGACTTCTTCCGGCTGAATCCCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACa                                                                                                             <  1:8375/150‑1 (MQ=255)
                                          aCCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGC‑AATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACa                                                                                                             >  1:3024272/1‑150 (MQ=255)
                                             gCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGC‑AATTACGCGCTCGACTTCTTCCGGCTGAATCCCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATg                                                                                                          <  1:521120/150‑1 (MQ=255)
                                              ccACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGC‑AATTACGCGCTCGACTTCTTCCGGCTGAATCCCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATgg                                                                                                         <  2:3263117/150‑1 (MQ=255)
                                              ccACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGC‑AATTACGCGCTCGACTTCTTCCGGCTGAATCCCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATgg                                                                                                         <  1:493239/150‑1 (MQ=255)
                                                 ccGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGC‑AATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCa                                                                                                      >  1:1959570/1‑150 (MQ=255)
                                                  cGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGC‑AATTACGCGCTCGACTTCTTCCGGCTGAATCCCCTATCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAg                                                                                                     <  1:76315/150‑1 (MQ=255)
                                                  cGGTCGATGACCAAACCCCTTGTCGGCAACGGGGACGATAAACCCCTGCAAAACCGCAGGATGTGCAGC‑AATTACGCGCTCGACTTCTTCCGGCTGAATCCCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAg                                                                                                     <  2:3511280/150‑1 (MQ=255)
                                                     tCGATGACCAACCTCTTTGCGGGCAGCGGGGACAAGAACCCCCTGCAAAACCGCGGAATGTGCACC‑AATAACGCCATCGCCTTCTCCCGGCGAAAAACCCTTTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCtt                                                                                                  <  2:294519/150‑1 (MQ=255)
                                                     tCGATGACCAACCTCCTTGTCGGCAAGGGGGACGATAAACCCCTGCAAAACCGCAGGATGTGCAGC‑AATTACGCGCTCGACTTCTTCCGGCTGAATCCCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCtt                                                                                                  <  2:2539401/150‑1 (MQ=255)
                                                     tCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAACCCCTGCAAACCCGCAGGATGTGCAGC‑AATTACGCGCTCGACTTCTTCCGGCTGAATCCCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCtt                                                                                                  <  2:285907/150‑1 (MQ=255)
                                                      cGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGC‑AATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTg                                                                                                 >  1:2877858/1‑150 (MQ=255)
                                                          gACCAAACTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGC‑AATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCAt                                                                                             >  1:3557305/1‑150 (MQ=255)
                                                           aCCAAACTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGC‑AATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCAtt                                                                                            >  2:2341766/1‑150 (MQ=255)
                                                            ccAACCTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGC‑AATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTa                                                                                           <  1:3727357/150‑1 (MQ=255)
                                                            ccAAACTCCTTGCCGGCAACGGGGACGATAAACCCCTGCAAAACCGCAGAATGTCCAGC‑AATTACCCGCTCGTCTTCTTCCGGCTGATTCCCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTa                                                                                           <  2:733187/150‑1 (MQ=255)
                                                              aaccTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGC‑AATTACGCGCTCGACTTCTTCCGGCTGAATCCCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCCTGCCATTATg                                                                                         <  1:700896/147‑1 (MQ=255)
                                                              aaaCTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGC‑AATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATg                                                                                         >  2:2272387/1‑150 (MQ=255)
                                                               accTCCTTGTCGGAAACGGGGACGATAAACACCTGCAAACCCGCAGGATGTGCAGC‑AATTACGCGCTCGACTTCTTCCGGCTGAATCCCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGc                                                                                        <  2:418233/148‑1 (MQ=255)
                                                               aaCTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGC‑AATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGc                                                                                        >  2:852716/1‑150 (MQ=255)
                                                                aCTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGC‑AATTACGCGCTCGACTTCTTCCGGCTGAATCCCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCa                                                                                       <  1:3379941/150‑1 (MQ=255)
                                                                aCTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGC‑AATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCa                                                                                       >  2:1543215/1‑150 (MQ=255)
                                                                       gTCGGCAACGGGGACGATAAACCCCTGCAAAACCGCAGGATGTGCAGC‑AATTACGCGCTCGACTTCTTCCGGCTGAATCCCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCAcc                                                                                <  2:3081900/150‑1 (MQ=255)
                                                                       gTCGGCAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGC‑AATTCCGCGCTCGACTTCTTCCGGCTGAATCCCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCAcc                                                                                <  2:1457529/150‑1 (MQ=255)
                                                                        tCGGCAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGC‑AATTACGCGCTCGACTTCTCCCGGCTGAATCCCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACcg                                                                               <  2:2456973/150‑1 (MQ=255)
                                                                         cGGCAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGC‑AATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACcgc                                                                              >  2:170831/1‑150 (MQ=255)
                                                                             aaCGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGC‑AATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATc                                                                          >  2:12698/1‑150 (MQ=255)
                                                                             aaCGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGC‑AATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATc                                                                          >  1:2771722/1‑150 (MQ=255)
                                                                             aaCGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGC‑AATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATc                                                                          >  1:3280933/1‑150 (MQ=255)
                                                                              aCGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGC‑AATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATcg                                                                         >  1:4014602/1‑150 (MQ=255)
                                                                                   gACGATAAACCCCTGCAAAACCGCAGGATGTGCAGC‑AATTACGCGCTCGACTTCTTCCGGCTGAATCCCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCgcgt                                                                    <  2:709332/150‑1 (MQ=255)
                                                                                   gACGATAAACACCTGCAAAACCGCAGGATGTGCAGC‑AATTACGCGCTCGACTTCTTCCGGCTGAATCCCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCgcgt                                                                    <  1:3644326/150‑1 (MQ=255)
                                                                                     cGATAAACCCCTGCAAAACCGCAGGATGTGCAGC‑AATTACGCGCTCGACTTCTTCCGGCTGAATCCCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCgcgtag                                                                  <  2:2792492/150‑1 (MQ=255)
                                                                                      gATAACCCCCTGCAAACCCGCAGGATGTGCAGC‑AATTACGCGCTCGACTTCTTCCGGCTGAATCCCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCgcgtagc                                                                 <  2:3749757/150‑1 (MQ=255)
                                                                                       aTAAACACCTGCAAAACCGCAGGATGTGCAGC‑AATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCgcgtagcg                                                                <  1:240240/150‑1 (MQ=255)
                                                                                       aTAAACACCTGCAAAACCGCAGGATGTGCAGC‑AATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCgcgtagcg                                                                >  1:1125182/1‑150 (MQ=255)
                                                                                        tAAACACCTGCAAAACCGCAGGATGTGCAGA‑AATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCgcgtagcgt                                                               >  2:1961623/1‑150 (MQ=255)
                                                                                               cTGCAAAACCGCAGGATGTGCAGC‑AATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGcc                                                        >  1:207880/1‑150 (MQ=255)
                                                                                                tGCAAAACCGCAGGATGTGCAGC‑AATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCt                                                       <  1:2608073/150‑1 (MQ=255)
                                                                                                tGCAAAACCGCAGGATGTGCAGC‑AATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCt                                                       <  1:3505284/150‑1 (MQ=255)
                                                                                                 gCAAAACCGCAGGATGTGCAGC‑AATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCtt                                                      >  1:3331593/1‑150 (MQ=255)
                                                                                                     aaCCGCAGGATGTGCAGC‑AATTACGCGCTCGACTTCTTCCGGCTGAATCCCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCTTCGTc                                                  <  1:1898365/150‑1 (MQ=255)
                                                                                                       ccGCAGGTTGTGCAGC‑AATACCCCGCCCGCTTTTTCCCGGCTAAACCCCCCCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCTTCGTCAt                                                <  2:3038179/150‑1 (MQ=255)
                                                                                                         gCAGGATGTGCAGC‑AATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCTTCGTCATTa                                              >  1:1417274/1‑150 (MQ=255)
                                                                                                          cAGGATGTGCAGC‑AATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCTTCGTCATTaa                                             <  1:3215224/150‑1 (MQ=255)
                                                                                                               tgtgCAGC‑AATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCTTCGTCATTAACCAGt                                        >  2:3443391/1‑150 (MQ=255)
                                                                                                               tgtgCAGC‑AATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCTTCGTCATTAACCAGt                                        >  1:1380093/1‑150 (MQ=255)
                                                                                                                 tgCAGC‑AATTCCGCGCTCGACTTCTTCCGGCTGAATCCCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCTTCGTCATTAACCAGTGa                                      <  1:2574767/150‑1 (MQ=255)
                                                                                                                    agc‑aATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCTTCGTCATTAACCAGTGAAAc                                   >  1:1018733/1‑150 (MQ=255)
                                                                                                                     gc‑aATTACGCGCTCGACTTCTCCCGGCTGAATCCCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCTTCGTCATTAACCAGTGAAAcc                                  <  2:1019653/150‑1 (MQ=255)
                                                                                                                      c‑aATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCTTCGTCATTAACCAGTGAAACCa                                 >  2:3609688/1‑150 (MQ=255)
                                                                                                                        aaTTACGCGCTCGACTTCTTCCGGCTGAATCCCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCTTCGTCATTAACCAGTGAAACCAg                                <  2:1079125/150‑1 (MQ=255)
                                                                                                                        aaTTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCTTCGTCATTAACCAGTGAAACCAg                                >  2:915143/1‑150 (MQ=255)
                                                                                                                          ttccggtcTCAACTTCCTCCGGCAGAATCCCCCCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCTTCGTCATTAACCAGTGAAACCAGtt                              <  2:1884259/143‑1 (MQ=255)
                                                                                                                                gcTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCTTCGTCATTAACCAGTGAAACCAGTTGCCCGt                        >  2:1582894/1‑150 (MQ=255)
                                                                                                                                gcTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCTTCGTCATTAACCAGTGAAACCAGTTGCCCGt                        >  1:3188313/1‑150 (MQ=255)
                                                                                                                                 cTCGACTTCTTCCGGCTGAATCCCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCTTCGTCATTAACCAGTGAAACCAGTTGCCCGtt                       <  1:2179253/150‑1 (MQ=255)
                                                                                                                                 cTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCTTCGTCATTAACCAGTGAAACCAGTTGCCCGtt                       >  1:1616990/1‑150 (MQ=255)
                                                                                                                                   cGACTTCTTCCGGCTGAATACCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCTTCGTCATTAACCAGTGAAACCAGTTGCCCGTTAc                     >  1:1737358/1‑150 (MQ=255)
                                                                                                                                       ttcttcCGGCTGAATACCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCTTCGTCATTAACCAGTGAAACCAGTTGCCCGTTACGCCa                 >  2:3996663/1‑150 (MQ=255)
                                                                                                                                       ttcttcCGGCTAAATCCCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCTTCGTCATTAACCAGTGAAACCAGTTGCCCGTTACGCCa                 <  2:1372555/150‑1 (MQ=255)
                                                                                                                                        tcttcCGGCTGAATACCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCTTCGTCATTAACCAGTGAAACCAGTTGCCCGTTACGCCAg                >  1:1511573/1‑150 (MQ=255)
                                                                                                                                        tcttcCGGCTGAATACCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCTTCGTCATTAACCAGTGAAACCAGTTGCCCGTTACGCCAg                >  1:654907/1‑150 (MQ=255)
                                                                                                                                        tctccCGGCTGAATCCCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCTTCGTCATTAACCAGTGAAACCAGTTGCCCGTTACGCCAg                <  2:1413046/150‑1 (MQ=255)
                                                                                                                                          ttcCGGCTGAATCCCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCTTCGTCATTAACCAGTGAAACCAGTTGCCCGTTACGCCAGTa              <  1:3957036/150‑1 (MQ=255)
                                                                                                                                            ccGGCTGAATACCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCTTCGTCATTAACCAGTGAAACCAGTTGCCCGTTACGCCAGTAAc            >  2:2164122/1‑150 (MQ=255)
                                                                                                                                             cGGCTGAATACCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCTTCGTCATTAACCAGTGAAACCAGTTGCCCGTTACGCCAGTAAcc           >  1:1084397/1‑150 (MQ=255)
                                                                                                                                              ggCTAAATCCCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCTTCGTCATTAACCAGTGAAACCAGTTGCCCGTTACGCCAGTAACCt          <  2:3898269/150‑1 (MQ=255)
                                                                                                                                                cTGAATCCCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCTTCGTCATTAACCAGTGAAACCAGTTGCCCGTTACGCCAGTAACCTTc        <  2:2618503/150‑1 (MQ=255)
                                                                                                                                                cTGAATACCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCTTCGTCATTAACCAGTGAAACCAGTTGCCCGTTACGCCAGTAACCTTc        <  2:3147/150‑1 (MQ=255)
                                                                                                                                                  gAATCCCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCTTCGTCATTAACCAGTGAAACCAGTTGCCCGTTACGCCAGTAACCTTCTg      <  2:1799559/150‑1 (MQ=255)
                                                                                                                                                    aTACCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCTTCGTCATTAACCAGTGAAACCAGTTGCCCGTTACGCCAGTAACCTTCTGcc    <  1:236144/150‑1 (MQ=255)
                                                                                                                                                      aCCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCTTCGTCATTAACCAGTGAAACCAGTTGCCCGTTACGCCAGTAACCTTCTGCCAt  >  2:3658690/1‑150 (MQ=255)
                                                                                                                                                      |                                                                                                                                                     
ACCCATTCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGC‑AATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCTTCGTCATTAACCAGTGAAACCAGTTGCCCGTTACGCCAGTAACCTTCTGCCAT  >  AP012306/1944139‑1944437

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑