BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AP012306	343,414	T→A	F13Y (TTT→TAT)	thiL →	thiamin‑monophosphate kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AP012306	343,414	0	T	A	100.0%	1022.5 / NA	255	F13Y (TTT→TAT)	thiL	thiamin‑monophosphate kinase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base A (137/118); total (137/118)

Only 100 of 261 total aligned reads displayed.

GCAGCACCTGTGATTCGCCCTAACAAAAAGTGATATCCAGGCCGGTAGATTCACGGAAGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCGTTATTTTGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTGCGCACTTCTCAATATCCCCGAGAAACAGACCCTGGCGATCAGCACTGATACGCTGGTGGCGGGTAACCATTTCCTCCCTGATATCGATCCTG  >  AP012306/343267‑343560

   gcaCCTGTGATTCGCCCTAACAAAAAGTGATATCCAGGCCGGTAGATTCACGGAAGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCGTTATTATGAcc                                                                                                                                               >  2:1121869/1‑150 (MQ=255)

    caCCTGTGATTCGCCCTAACAAAAAGTGATATCCAGGCCGGTAGATTCACGGAAGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCGTTATTATGACCg                                                                                                                                              >  1:514101/1‑150 (MQ=255)

     aCCTGTGATTCGCCCTAACAAAAAGTGATATCCAGGCCGGTAGATTCACGGAAGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCGTTATTATGACCgt                                                                                                                                             <  2:3071706/150‑1 (MQ=255)

       cTGTGATTCGCCCTAACAAAAAGTGATATCCAGGCCGGTAGATTCACGGAAGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCGTTATTATGACCgtgt                                                                                                                                           >  2:3271869/1‑150 (MQ=255)

       cTGTGATTCGCCCTAACAAAAAGTGATATCCAGGCCGGTAGATTCACGGAAGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCGTTATTATCACCgtgt                                                                                                                                           >  1:2581520/1‑150 (MQ=255)

        tgtgATTCGCCCTAACAAAAAGTGATATCCAGGCCGGTAGATTCACGGAAGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCGTTATTATGACCGTGTa                                                                                                                                          >  2:2646796/1‑150 (MQ=255)

         gtgATTCGCCCTAACAAAAAGTGATATCCAGGCCGGTAGATTCACGGAAGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCGTTATTATGACCGTGTaa                                                                                                                                         <  2:1032338/150‑1 (MQ=255)

               cGCCCTAACAAAAAGTGATATCCAGGCCGGTAGATTCACGGAAGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCGTTATTATGACCGTGTAAGAAGtt                                                                                                                                   >  1:3257895/1‑150 (MQ=255)

               cGCCCTAACAAAAAGTGATATCCAGGCCGGTAGATTCACGGAAGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCGTTATTATGACCGTGTAAGAAGtt                                                                                                                                   <  1:3492515/150‑1 (MQ=255)

               cGCCCTAACAAAAAGTGATATCCAGGCCGGTAGATTCACGGAAGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCGTTATTATGACCGTGTAAGAAGtt                                                                                                                                   <  1:872049/150‑1 (MQ=255)

               cGCCCTAACAAAAAGTGATATCCAGGCCGGTAGATTCACGGAAGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCGTTATTATGACCGTGTAAGAAGtt                                                                                                                                   >  2:3649517/1‑150 (MQ=255)

               cGCCCTAACAAAAAGTGATATCCAGGCCGGTAGATTCACGGAAGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCGTTATTATGACCGTGTAAGAAGtt                                                                                                                                   <  2:1662446/150‑1 (MQ=255)

                gCCCTAACAAAAAGTGATATCCAGGCCGGTAGATTCACGGAAGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCGTTATTATGACCGTGTAAGAAGTtc                                                                                                                                  >  2:1647109/1‑150 (MQ=255)

                  ccTAACAAAAAGTGATATCCAGGCCGGTAGATTCACGGAAGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCGTTATTATGACCGTGTAAGAAGTtctc                                                                                                                                >  1:3523424/1‑150 (MQ=255)

                   cTAACAAAAAGTGATATCCAGGCCGGTAGATTCACGGAAGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCGTTATTATGACCGTGTAAGAAGTTCTCg                                                                                                                               <  2:2467682/150‑1 (MQ=255)

                    tAACAAAAAGTGATATCCAGGCCGGTAGATTCACGGAAGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCGTTATTATGACCGTGTAAGAAGTTCTCGt                                                                                                                              >  1:1607385/1‑150 (MQ=255)

                       cAAAAAGTGATATCCAGGCCGGTAGATTCACGGAAGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCGTTATTATGACCGTGTAAGAAGTTCTCGTCtt                                                                                                                           <  2:522147/150‑1 (MQ=255)

                       cAAAAAGTGATATCCAGGCCGGTAGATTCACGGAAGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCGTTATTATGACCGTGTAAGAAGTTCTCGTCtt                                                                                                                           >  2:655351/1‑150 (MQ=255)

                          aaaGTGATATCCAGGCCGGTAGATTCACGGAAGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCGTTATTATGACCGTGTAAGAAGTTCTCGTCTTGAt                                                                                                                        <  1:2717105/150‑1 (MQ=255)

                           aaGTGATATCCAGGCCGGTAGATTCACGGAAGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCGTTATTATGACCGTGTAAGAAGTTCTCGTCTTGATg                                                                                                                       <  2:2508375/150‑1 (MQ=255)

                            aGTGATATCCAGGCCGGTAGATTCACGGAAGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCGTTATTATGACCGTGTAAGAAGTTCTCGTCTTGATGt                                                                                                                      <  2:4078806/150‑1 (MQ=255)

                            aGTGATATCCAGGCCGGTAGATTCACGGAAGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCGTTATTATGACCGTGTAAGAAGTTCTCGTCTTGATGt                                                                                                                      >  2:886705/1‑150 (MQ=255)

                               gATATCCAGGCCGGTAGATTCACGGAAGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCGTTATTATGACCGTGTAAGAAGTTCTCGTCTTGATGTCGa                                                                                                                   >  2:2132150/1‑150 (MQ=255)

                                atatCCAGGCCGGTAGATTCACGGAAGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCGTTATTATGACCGTGTAAGAAGTTCTCGTCTTGATGTCGaa                                                                                                                  <  2:3562607/150‑1 (MQ=255)

                                atatCCAGGCCGGTAGATTCACGGAAGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCGTTATTATGACCGTGTAAGAAGTTCTCGTCTTGATGTCGaa                                                                                                                  >  2:4100388/1‑150 (MQ=255)

                                 tatCCAGGCCGGTAGATTCACGGAAGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCGTTATTATGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAAc                                                                                                                 <  2:2059422/150‑1 (MQ=255)

                                  atCCAGGCCGGTAGATTCACGGAAGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCGTTATTATGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACt                                                                                                                >  2:927427/1‑150 (MQ=255)

                                  atCCAGGCCGGTAGATTCACGGAAGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCGTTATTATGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACt                                                                                                                >  2:324399/1‑150 (MQ=255)

                                     cAGGCCGGTAGATTCACGGAAGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCGTTATTATGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTggg                                                                                                             <  2:468031/150‑1 (MQ=255)

                                     cAGGCCGGTAGATTCACGGAAGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCGTTATTATGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTggg                                                                                                             >  1:1771732/1‑150 (MQ=255)

                                        gCCGGTAGATTCACGGAAGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCGTTATTATGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCAt                                                                                                          >  1:1584861/1‑150 (MQ=255)

                                         ccGGTAGATTCACGGAAGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCGTTATTATGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATc                                                                                                         >  2:1722221/1‑150 (MQ=255)

                                         ccGGTAGATTCACGGAAGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCGTTATTATGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATc                                                                                                         >  1:1339506/1‑150 (MQ=255)

                                         ccGGTAGATTCACGGAAGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCGTTATTATGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATc                                                                                                         >  2:3970957/1‑150 (MQ=255)

                                           ggTAGATTCACGGAAGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCGTTATTATGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCgg                                                                                                       <  1:4108919/150‑1 (MQ=255)

                                           ggTAGATTCACGGAAGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCGTTATTATGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCgg                                                                                                       <  1:2286229/150‑1 (MQ=255)

                                           ggTAGATTCACGGAAGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCGTTATTATGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCgg                                                                                                       <  1:1805804/150‑1 (MQ=255)

                                            gTAGATTCACGGAAGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCGTTATTATGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGc                                                                                                      <  1:2864738/150‑1 (MQ=255)

                                             tAGATTCACGGAAGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCGTTATTATGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGcg                                                                                                     >  2:1063231/1‑150 (MQ=255)

                                               gATTCACGGAAGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCGTTATTATGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGcgac                                                                                                   <  1:3084075/150‑1 (MQ=255)

                                                  tCACGGAAGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCGTTATTATGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGAt                                                                                                <  1:2031357/150‑1 (MQ=255)

                                                    aCGGAAGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCGTTATTATGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTg                                                                                              >  2:2353078/1‑150 (MQ=255)

                                                     cGGAAGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCGTTATTATGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTgc                                                                                             <  2:3896696/150‑1 (MQ=255)

                                                      ggAAGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCGTTATTATGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTgcg                                                                                            <  1:3142031/150‑1 (MQ=255)

                                                         aGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCGTTATTATGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTGCGCAc                                                                                         <  1:1084101/150‑1 (MQ=255)

                                                           aCCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCGTTATTATGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTGCGCACtt                                                                                       >  1:3366886/1‑150 (MQ=255)

                                                               ttCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCGTTATTATGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTGCGCACTTCTCa                                                                                   <  2:3744436/150‑1 (MQ=255)

                                                               ttCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCGTTATTATGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTGCGCACTTCTCa                                                                                   <  2:2427585/150‑1 (MQ=255)

                                                               ttCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCGTTATTATGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTGCGCACTTCTCa                                                                                   >  2:1876044/1‑150 (MQ=255)

                                                               ttCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCGTTATTATGACCGCGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTGCGCACTTCTCa                                                                                   >  2:294826/1‑150 (MQ=255)

                                                                tCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCGTTATTATGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTGCGCACTTCTCaa                                                                                  >  2:2722397/1‑150 (MQ=255)

                                                                  cATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCGTTATTATGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTGCGCACTTCTCAata                                                                                >  1:3949170/1‑150 (MQ=255)

                                                                   aTGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCGTTATTATGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTGCGCACTTCTCAatat                                                                               <  1:1951515/150‑1 (MQ=255)

                                                                    tGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCGTTATTATGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTGCGCACTTCTCAATATc                                                                              >  1:2938430/1‑150 (MQ=255)

                                                                      aTCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCGTTATTATGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTGCGCACTTCTCAATATccc                                                                            >  2:348607/1‑150 (MQ=255)

                                                                       tCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCGTTATTATGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTGCGCACTTCTCAATATcccc                                                                           <  2:2437563/150‑1 (MQ=255)

                                                                           cGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCGTTATTATGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTGCGCACTTCTCAATATCCCCgaga                                                                       >  1:4062643/1‑150 (MQ=255)

                                                                             gCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCGTTATTATGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTGCGCACTTCTCAATATCCCCGAGaaa                                                                     <  1:1472540/150‑1 (MQ=255)

                                                                               cttttcttttTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCGTTATTATGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTGCGCACTTCTCAATATCCCCGAGAAACa                                                                   <  2:2174740/150‑1 (MQ=255)

                                                                                 tttcttttTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCGTTATTATGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTGCGCACTTCTCAATATCCCCGAGAAACAGa                                                                 >  1:618558/1‑150 (MQ=255)

                                                                                   tcttttTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCGTTATTATGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTGCGCACTTCTCAATATCCCCGAGAAACAGAcc                                                               >  2:2789803/1‑150 (MQ=255)

                                                                                    cttttTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCGTTATTATGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTGCGCACTTCTCAATATCCCCGAGAAACAGAccc                                                              >  2:3878924/1‑150 (MQ=255)

                                                                                      tttttACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCGTTATTATGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTGCGCACTTCTCAATATCCCCGAGAAACAGACCCTg                                                            <  1:2140748/150‑1 (MQ=255)

                                                                                      tttttACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCGTTATTATGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTGCGCACTTCTCAATATCCCCGAGAAACAGACCCTg                                                            >  2:2305662/1‑150 (MQ=255)

                                                                                         ttACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCGTTATTATGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTGCGCACTTCTCAATATCCCCGAGAAACAGACCCTGGCg                                                         >  1:30001/1‑150 (MQ=255)

                                                                                           aCCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCGTTATTATGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTGCGCACTTCTCAATATCCCCGAGAAACAGACCCTGGCGAt                                                       >  1:1182252/1‑150 (MQ=255)

                                                                                            ccTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCGTTATTATGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTGCGCACTTCTCAATATCCCCGAGAAACAGACCCTGGCGATc                                                      <  1:230904/150‑1 (MQ=255)

                                                                                            ccTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCGTTATTATGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTGCGCACTTCTCAATATCCCCGAGAAACAGACCCTGGCGATc                                                      >  1:1380737/1‑150 (MQ=255)

                                                                                             ctgctgAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCGTTATTATGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTGCGCACTTCTCAATATCCCCGAGAAACAGACCCTGGCGATCa                                                     <  1:265307/150‑1 (MQ=255)

                                                                                                ctgAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCGTTATTATGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTGCGCACTTCTCAATATCCCCGAGAAACAGACCCTGGCGATCAGCa                                                  <  1:3783457/150‑1 (MQ=255)

                                                                                                ctgAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCGTTATTATGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTGCGCACTTCTCAATATCCCCGAGAAACAGACCCTGGCGATCAGCa                                                  <  2:3621581/150‑1 (MQ=255)

                                                                                                ctgAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCGTTATTATGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTGCGCACTTCTCAATATCCCCGAGAAACAGACCCTGGCGATCAGCa                                                  >  1:777962/1‑150 (MQ=255)

                                                                                                      cATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCGTTATTATGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTGCGCACTTCTCAATATCCCCGAGAAACAGACCCTGGCGATCAGCACTGATa                                            >  2:3285723/1‑150 (MQ=255)

                                                                                                          aCGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCGTTATTATGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTGCGCACTTCTCAATATCCCCGAGAAACAGACCCTGGCGATCAGCACTGATACGCt                                        >  1:2613575/1‑150 (MQ=255)

                                                                                                              aTGGCATGTGGCGAGTTCTCCCTGATTGCCCGTTATTATGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTGCGCACTTCTCAATATCCCCGAGAAACAGACCCTGGCGATCAGCACTGATACGCtggtg                                    >  2:148912/1‑150 (MQ=255)

                                                                                                                ggCATGTGGCGAGTTCTCCCTGATTGCCCGTTATTATGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTGCGCACTTCTCAATATCCCCGAGAAACAGACCCTGGCGATCAGCACTGATACGCTGGTGGc                                  <  2:1362945/150‑1 (MQ=255)

                                                                                                                 gCATGTGGCGAGTTCACCCTGATTGCCCGTTATTATGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTGCGCACTTCTCAATATCCCCGAGAAACAGACCCTGGCGATCAGCACTGATACGCTGGTGGCg                                 <  2:3355373/150‑1 (MQ=255)

                                                                                                                   aTGTGGCGAGTTCTCCCTGATTGCCCGTTATTATGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTGCGCACTTCTCAATATCCCCGAGAAACAGACCCTGGCGATCAGCACTGATACGCTGGTGGCggg                               >  1:2414779/1‑150 (MQ=255)

                                                                                                                      tgGCGAGTTCTCCCTGATTGCCCGTTATTATGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTGCGCACTTCTCAATATCCCCGAGAAACAGACCCTGGCGATCAGCACTGATACGCTGGTGGCGGGTaa                            <  2:3026248/150‑1 (MQ=255)

                                                                                                                       ggCGAGTTCTCCCTGATTGCCCGTTATTATGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTGCGCACTTCTCAATATCCCCGAGAAACAGACCCTGGCGATCAGCACTGATACGCTGGTGGCGGGTAAc                           >  1:3735686/1‑150 (MQ=255)

                                                                                                                          gAGTTCTCCCTGATTGCCCGTTATTATGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTGCGCACTTCTCAATATCCCCGAGAAACAGACCCTGGCGATCAGCACTGATACGCTGGTGGCGGGGAAcccc                        >  2:798316/1‑148 (MQ=255)

                                                                                                                           aGTTCTCCCTGATTGCCCGTTATTATGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTGCGCACTTCTCAATATCCCCGAGAAACAGACCCTGGCGATCAGCACTGATACGCTGGTGGCGGGTAACCAtt                       <  1:69838/150‑1 (MQ=255)

                                                                                                                              tctcCCTGATTGCCCGTTATTATGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTGCGCACTTCTCAATATCCCCGAGAAACAGACCCTGGCGATCAGCACTGATACGCTGGTGGCGGGTAACCATTtcc                    <  2:3987333/150‑1 (MQ=255)

                                                                                                                              tctcCCTGATTGCCCGTTATTATGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTGCGCACTTCTCAATATCCCCGAGAAACAGACCCTGGCGATCAGCACTGATACGCTGGTGGCGGGTAACCATTtcc                    <  1:576166/150‑1 (MQ=255)

                                                                                                                               ctcCCTGATTGCCCGTTATTATGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTGCGCACTTCTCAATATCCCCGAGAAACAGACCCTGGCGATCAGCACTGATACGCTGGTGGCGGGTAACCATTtcct                   <  2:424448/150‑1 (MQ=255)

                                                                                                                               ctcCCTGATTGCCCGTTATTATGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTGCGCACTTCTCAATATCCCCGAGAAACAGACCCTGGCGATCAGCACTGATACGCTGGTGGCGGGTAACCATTtcct                   >  1:3539206/1‑150 (MQ=255)

                                                                                                                               ctcCCTGATTGCCCGTTATTATGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTGCGCACTTCTCAATATCCCCGAGAAACAGACCCTGGCGATCAGCACTGATACGCTGGTGGCGGGTAACCATTtcct                   >  1:1544587/1‑150 (MQ=255)

                                                                                                                                tcCCTGATTGCCCGTTATTATGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTGCGCACTTCTCAATATCCCCGAGAAACAGACCCTGGCGATCAGCACTGATACGCTGGTGGCGGGTAACCATTtcctc                  <  1:3996520/150‑1 (MQ=255)

                                                                                                                                tcCCTGATTGCCCGTTATTATGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTGCGCACTTCTCAATATCCCCGAGAAACAGACCCTGGCGATCAGCACTGATACGCTGGTGGCGGGTAACCATTtcctc                  >  2:2595087/1‑150 (MQ=255)

                                                                                                                                 cccTGATTGCCCGTTATTATGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTGCGCACTTCTCAATATCCCCGAGAAACAGACCCTGGCGATCAGCACTGATACGCTGGTGGCGGGGAACCATTtcctcc                 >  2:1560934/1‑150 (MQ=255)

                                                                                                                                 cccTGATTGCCCGTTATTATGACCGTGTAAGAAGTTATCGTCTTGATGTCGAACTGGGCATCGGCGACGATTGCGCACTTCTCAATATCCCCGAGAAACAGACCCTGGCGATCAGCACTGATACGCTGGTGGCGGGTAACCATTtcctcc                 <  1:1906205/150‑1 (MQ=255)

                                                                                                                                    tGATTGCCCGTTATTATGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTGCGCACTTCTCAATATCCCCGAGAAACAGACCCTGGCGATCAGCACTGATACGCTGGTGGCGGGTAACCATTTCCTCCCTg              <  1:3681759/150‑1 (MQ=255)

                                                                                                                                    tGATTGCCCGTTATTATGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTGCGCACTTCTCAATATCCCCGAGAAACAGACCCTGGCGATCAGCACTGATACGCTGGTGGCGGGTAACCATTTCCTCCCTg              <  1:2785353/150‑1 (MQ=255)

                                                                                                                                         gCCCGTTATTATGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTGCGCACTTCTCAATATCCCCGAGAAACAGACCCTGGCGATCAGCACTGATACGCTGGTGGCGGGTAACCATTTCCTCCCTGATATc         >  1:1279434/1‑150 (MQ=255)

                                                                                                                                           ccGTTATTATGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTGCGCACTTCTCAATATCCCCGAGAAACAGACCCTGGCGATCAGCACTGATACGCTGGTGGCGGGTAACCATTTCCTCCCTGATATCGa       <  2:1790602/150‑1 (MQ=255)

                                                                                                                                            cGTTATTATGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTGCGCACTTCTCAATATCCCCGAGAAACAGACCCTGGCGATCAGCACTGATACGCTGGTGGCGGGTAACCATTTCCTCCCTGATATCGAt      >  1:3278968/1‑150 (MQ=255)

                                                                                                                                               tattatGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTGCGCACTTCTCAATATCCCCGAGAAACAGACCCTGGCGATCAGCACTGATACGCTGGTGGCGGGTAACCATTTCCTCCCTGATATCGATCct   <  1:2344306/150‑1 (MQ=255)

                                                                                                                                                aTTATGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTGCGCACTTCTCAATATCCCCGAGAAACAGACCCTGGCGATCAGCACTGATACGCTGGTGGCGGGTAACCATTTCCTCCCTGATATCGATCctg  >  2:2910286/1‑150 (MQ=255)

                                                                                                                                                aTTATGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTGCGCACTTCTCAATATCCCCGAGAAACAGACCCTGGCGATCAGCACTGATACGCTGGTGGCGGGTAACCATTTCCTCCCTGATATCGATCctg  >  1:2199320/1‑150 (MQ=255)

                                                                                                                                                aTTATGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTGCGCACTTCTCAATATCCCCGAGAAACAGACCCTGGCGATCAGCACTGATACGCTGGTGGCGGGTAAACATTTCCTCCCTGATATCGATcccg  >  1:3041526/1‑148 (MQ=255)

GCAGCACCTGTGATTCGCCCTAACAAAAAGTGATATCCAGGCCGGTAGATTCACGGAAGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCGTTATTTTGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTGCGCACTTCTCAATATCCCCGAGAAACAGACCCTGGCGATCAGCACTGATACGCTGGTGGCGGGTAACCATTTCCTCCCTGATATCGATCCTG  >  AP012306/343267‑343560

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`