Predicted mutation
evidence seq id position mutation annotation gene description
RA AP012306 1,583,643 T→A I139N (ATC→AAC)  manY → mannose‑specific enzyme IIC component of PTS

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123061,583,6430TA100.0% 974.2 / NA 254I139N (ATC→AAC) manYmannose‑specific enzyme IIC component of PTS
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (88/166);  total (88/166)

Only 100 of 256 total aligned reads displayed.

AGAGCATTGGTGCAGGTATCGCACTGGCAATCCCTCTGGCCGCTGCGGGCCAGGTACTGACCATCATCGTTCGTACTATTACCGTTGCTTTCCAGCACGCTGCGGATAAGGCTGCTGATAACGGCAACCTGACAGCGATTTCCTGGATCCACGTTTCTTCTCTGTTCCTGCAAGCAATGCGTGTGGCTATTCCGGCCGTCATCGTTGCGCTGTCTGTTGGTACCAGCGAAGTACAGAACATGCTGAATGCGATTCCGGAAGTGGTGACCAATGGTCTGAATATCGCCGGTGGC  >  AP012306/1583496‑1583788
                                                                                                                                                   |                                                                                                                                                 
agagCATTGGTGCAGGTATCGCACTGGCAATCCCTCTGGCCGCTGCGGGCCAGGTACTGACCATCATCGTTCGTACTATTACCGTTGCTTTCCAGCACGCTGCGGATAAGGCTGCTGATAACGGCAACCTGACAGCGATTTCCTGGAAcc                                                                                                                                                 >  2:787701/1‑150 (MQ=255)
agagCATTGGTGCAGGTATCGCACTGGCAATCCCTCTGGCCGCTGCGGGCCAGGTACTGACCATCATCGTTCGTACTATTACCGTTGCTTTCCAGCACGCTGCGGATAAGGCTGCTGATAACGGCAACCTGACAGCGATTTCCTGGAAcc                                                                                                                                                 >  2:3080941/1‑150 (MQ=255)
agagCATTGGTGCAGGTATCGCACTGGCAATCCCTCTGGCCGCTGCGGGCCAGGTACTGACCATCATCGTTCGTACTATTACCGTTGCTTTCCAGCACGCTGCGGATAAGGCTGCTGATAACGGCAACCTGACAGCGATTTCCTGGAAcc                                                                                                                                                 >  2:4086694/1‑150 (MQ=255)
 gagCATTGGTGCAGGTATCGCACTGGCAATCCCTCTGGCCGCTGCGGGCCAGGTACTGACCATCATCGTTCGTACTATTACCGTTGCTTTCCAGCACGCTGCGGATAAGGCTGCTGATAACGGCAACCTGACAGCGATTTCCTGGAACCa                                                                                                                                                <  2:2693726/150‑1 (MQ=255)
  agCATTGGTGCAGGTATCGCACTGGCAATCCCTCTGGCCGCTGCGGGCCAGGTACTGACCATCATCGTTCGTACTATTACCGTTGCTTTCCAGCACGCTGCGGATAAGGCTGCTGATAACGGCAACCTGACAGCGATTTCCTGGAACCAc                                                                                                                                               >  1:2933575/1‑150 (MQ=255)
  agCATTGGTGCAGGTATCGCACTGGCAATCCCTCTGGCCGCTGCGGGCCAGGTACTGACCATCATCGTTCGTACTATTACCGTTGCTTTCCAGCACGCTGCGGATAAGGCTGCTGATAACGGCAACCTGACAGCGATTTCCTGGAACCAc                                                                                                                                               <  1:777947/150‑1 (MQ=255)
   gCATTGGTGCAGGTATCGCACTGGCAATCCCTCTGGCCGCTGCGGGCCAGGTACTGACCATCATCGTTCGTACTATTACCGTTGCTTTCCAGCACGCTGCGGATAAGGCTGCTGATAACGGCAACCTGACAGCGATTTCCTGGAACCACg                                                                                                                                              <  1:1340645/150‑1 (MQ=255)
     aTTGGTGCAGGTATCGCACTGGCAATCCCTCTGGCCGCTGCGGGCCAGGTACTGACCATCATCGTTCGTACTATTACCGTTGCTTTCCAGCACGCTGCGGATAAGGCTGCTGATAACGGCAACCTGACAGCGATTTCCTGGAACCACGtt                                                                                                                                            >  2:499626/1‑150 (MQ=255)
      ttGGTGCAGGTATCGCACTGGCAATCCCTCTGGCCGCTGCGGGCCAGGTACTGACCATCATCGTTCGTACTATTACCGTTGCTTTCCAGCACGCTGCGGATAAGGCTGCTGATAACGGCAACCTGACAGCGATTTCCTGGAACCACGttt                                                                                                                                           <  1:853625/150‑1 (MQ=255)
         gTGCAGGTATCGCACTGGCAATCCCTCTGGCCGCTGCGGGCCAGGTACTGACCATCATCGTTCGTACTATTACCGTTGCTTTCCAGCACGCTGCGGATAAGGCTGCTGATAACGGCAACCTGACAGCGATTTCCTGGAACCACGTttctt                                                                                                                                        <  2:3607230/150‑1 (MQ=255)
           gCAGGTATCGCACTGGCAATCCCTCTGGCCGCTGCGGGCCAGGTACTGACCATCATCGTTCGTACTATTACCGTTGCTTTCCAGCACGCTGCGGATAAGGCTGCTGATAACGGCAACCTGACAGCGATTTCCTGGAACCACGTTtcttct                                                                                                                                      <  1:3172116/150‑1 (MQ=255)
           gCAGGTATCGCACTGGCAATCCCTCTGGCCGCTGCGGGCCAGGTACTGACCATCATCGTTCGTACTATTACCGTTGCTTTCCAGCACGCTGCGGATAAGGCTGCTGATAACGGCAACCTGACAGCGATTTCCTGGAACCACGTTtcttct                                                                                                                                      <  1:1189937/150‑1 (MQ=255)
            cAGGTATCGCACTGGCAATCCCTCTGGCCGCTGCGGGCCAGGTACTGACCATCATCGTTCGTACTATTACCGTTGCTTTCCAGCACGCTGCGGATAAGGCTGCTGATAACGGCAACCTGACAGCGATTTCCTGGAACCACGTTTCTtctc                                                                                                                                     <  1:576404/150‑1 (MQ=255)
             aGGTATCGCACTGGCAATCCCTCTGGCCGCTGCGGGCCAGGTACTGACCATCATCGTTCGTACTATTACCGTTGCTTTCCAGCACGCTGCGGATAAGGCTGCTGATAACGGCAACCTGACAGCGATTTCCTGGAACCACGTTTCTTctct                                                                                                                                    <  1:2804175/150‑1 (MQ=255)
             aGGTATCGCACTGGCAATCCCTCTGGCCGCTGCGGGCCAGGTACTGACCATCATCGTTCGTACTATTACCGTTGCTTTCCAGCACGCTGCGGATAAGGCTGCTGATAACGGCAACCTGACAGCGATTTCCTGGAACCACGTTTCTTctct                                                                                                                                    <  1:428517/150‑1 (MQ=255)
              ggTATCGCACTGGCAATCCCTCTGGCCGCTGCGGGCCAGGTACTGACCATCATCGTTCGTACTATTACCGTTGCTTTCCAGCACGCTGCGGATAAGGCTGCTGATAACGGCAACCTGACAGCGATTTCCTGGAACCACGTTTCTTCTCTg                                                                                                                                   <  1:2196205/150‑1 (MQ=255)
              ggTATCGCACTGGCAATCCCTCTGGCCGCTGCGGGCCAGGTACTGACCATCATCGTTCGTACTATTACCGTTGCTTTCCAGCACGCTGCGGATAAGGCTGCTGATAACGGCAACCTGACAGCGATTTCCTGGAACCACGTTTCTTCTCTg                                                                                                                                   >  1:101242/1‑150 (MQ=255)
                tATCGCACTGGCAATCCCTCTGGCCGCTGCGGGCCAGGTACTGACCATCATCGTTCGTACTATTACCGTTGCTTTCCAGCACGCTGCGGATAAGGCTGCTGATAACGGCAACCTGACAGCGATTTCCTGGAACCACGTTTCTTCTCTGtt                                                                                                                                 <  2:231208/150‑1 (MQ=255)
                   cGCACTGGCAATCCCTCTGGCCGCTGCGGGCCAGGTACTGACCATCATCGTTCGTACTATTACCGTTGCTTTCCAGCACGCTGCGGATAAGGCTGCTGATAACGGCAACCTGACAGCGATTTCCTGGAACCACGTTTCTTCTCTGTTCCt                                                                                                                              <  1:2782812/150‑1 (MQ=255)
                   cGCACTGGCAATCCCTCTGGCCGCTGCGGGCCAGGTACTGACCATCATCGTTCGTACTATTACCGTTGCTTTCCAGCACGCTGCGGATAAGGCTGCTGATAACGGCAACCTGACAGCGATTTCCTGGAACCACGTTTCTTCTCTGTTCCt                                                                                                                              <  2:218728/150‑1 (MQ=255)
                    gCACTGGCAATCCCTCTGGCCGCTGCGGGCCAGGTACTGACCATCATCGTTCGTACTATTACCGTTGCTTTCCAGCACGCTGCGGATAAGGCTGCTGATAACGGCAACCTGACAGCGATTTCCTGGAACCACGTTTCTTCTCTGTTCCTg                                                                                                                             <  2:1379508/150‑1 (MQ=255)
                    gCACTGGCAATCCCTCTGGCCGCTGCGGGCCAGGTACTGACCATCATCGTTCGTACTATTACCGTTGCTTTCCAGCACGCTGCGGATAAGGCTGCTGATAACGGCAACCTGACAGCGATTTCCTGGAACCACGTTTCTTCTCTGTTCCTg                                                                                                                             <  2:1933629/150‑1 (MQ=255)
                    gCACTGGCAATCCCTCTGGCCGCTGCGGGCCAGGTACTGACCATCATCGTTCGTACTATTACCGTTGCTTTCCAGCACGCTGCGGATAAGGCTGCTGATAACGGCAACCTGACAGCGATTTCCTGGAACCACGTTTCTTCTCTGTTCCTg                                                                                                                             <  1:3286179/150‑1 (MQ=255)
                      aCTGGCAATCCCTCTGGCCGCTGCGGGCCAGGTACTGACCATCATCGTTCGTACTATTACCGTTGCTTTCCAGCACGCTGCGGATAAGGCTGCTGATAACGGCAACCTGACAGCGATTTCCTGGAACCACGTTTCTTCTCTGTTCCTgca                                                                                                                           <  2:3876326/150‑1 (MQ=255)
                       cTGGCAATCCCTCTGGCCGCTGCGGGCCAGGTACTGACCATCATCGTTCGTAATATTACCGTTGCTTTCCAGCACGCTGCGGATAAGGCTGCTGATAACGGCAACCTGACAGCGATTTCCTGGAACCACGTTTCGTCTCTGTTCCTgcaa                                                                                                                          >  2:3366824/1‑150 (MQ=255)
                        tGGCAATCCCTCTGGCCGCTGCGGGCCAGGTACTGACCATCATCGTTCGTACTATTACCGTTGCTTTCCAGCACGCTGCGGATAAGGCTGCTGATAACGGCAACCTGACAGCGATTTCCTGGAACCACGTTTCTTCTCTGTTCCTgcaag                                                                                                                         <  1:3790593/150‑1 (MQ=255)
                              tCCCTCTGGCCGCTGCGGGCCAGGTACTGACCATCATCGTTCGTACTATTACCGTTGCTTTCCAGCACGCTGCGGATAAGGCTGCTGATAACGGCAACCTGACAGCGATTTCCTGGAACCACGTTTCTTCTCTGTTCCTGCAAGCAATGc                                                                                                                   >  2:2384170/1‑150 (MQ=255)
                              tCCCTCTGGCCGCTGCGGGCCAGGTACTGACCATCATCGTTCGTACTATTACCGTTGCTTTCCAGCACGCTGCGGATAAGGCTGCTGATAACGGCAACCTGACAGCGATTTCCTGGAACCACGTTTCTTCTCTGTTCCTGCAAGCAATGc                                                                                                                   <  2:1700664/150‑1 (MQ=255)
                                ccTCTGGCCGCTGCGGGCCAGGTACTGACCATCATCGTTCGTACTATTACCGTTGCTTTCCAGCACGCTGCGGATAAGGCTGCTGATAACGGCAACCTGACAGCGATTTCCTGGAACCACGTTTCTTCTCTGTTCCTGCAAGCAATGCgt                                                                                                                 <  1:140053/150‑1 (MQ=255)
                                 ctctGGCCGCTGCGGGCCAGGTACTGACCATCATCGTTCGTACTATTACCGTTGCTTTCCAGCACGCTGCGGATAAGGCTGCTGATAACGGCAACCTGACAGCGATTTCCTGGAACCACGTTTCTTCTCTGTTCCTGCAAGCAATGCgtg                                                                                                                >  2:2268975/1‑150 (MQ=255)
                                  tctGGCCGCTGCGGGCCAGGTACTGACCATCATCGTTCGTACTATTACCGTTGCTTTCCAGCACGCTGCGGATAAGGCTGCTGATAACGGCAACCTGACAGCGATTTCCTGGAACCACGTTTCTTCTCTGTTCCTGCAAGCAATGCgtgt                                                                                                               <  2:2424488/150‑1 (MQ=255)
                                  tctGGCCGCTGCGGGCCAGGTACTGACCATCATCGTTCGTACTATTACCGTTGCTTTCCAGCACGCTGCGGATAAGGCTGCTGATAACGGCAACCTGACAGCGATTTCCTGGAACCACGTTTCTTCTCTGTTCCTGCAAGCAATGCgtgt                                                                                                               <  2:1846993/150‑1 (MQ=255)
                                    tGGCCGCTGCGGGCCAGGTACTGACCATCATCGTTCGTACTATTACCGTTGCTTTCCAGCACGCTGCGGATAAGGCTGCTGATAACGGCAACCTGACAGCGATTTCCTGGAACCACGTTTCTTCTCTGTTCCTGCAAGCAATGCGTGTgg                                                                                                             <  1:1951282/150‑1 (MQ=255)
                                    tGGCCGCTGCGGGCCAGGTACTGACCATCATCGTTCGTACTATTACCGTTGCTTTCCAGCACGCTGCGGATAAGGCTGCTGATAACGGCAACCTGACAGCGATTTCCTGGAACCACGTTTCTTCTCTGTTCCTGCAAGCAATGCGTGTgg                                                                                                             <  1:1223740/150‑1 (MQ=255)
                                    tGGCCGCTGCGGGCCAGGTACTGACCATCATCGTTCGTACTATTACCGTTGCTTTCCAGCACGCTGCGGATAAGGCTGCTGATAACGGCAACCTGACAGCGATTTCCTGGAACCACGTTTCTTCTCTGTTCCTGCAAGCAATGCGTGTgg                                                                                                             <  2:649892/150‑1 (MQ=255)
                                    tGGCCGCTGCGGGCCAGGTACTGACCATCATCGTTCGTACTATTACCGTTGCTTTCCAGCACGCTGCGGATAAGGCTGCTGATAACGGCAACCTGACAGCGATTTCCTGGAACCACGTTTCTTCTCTGTTCCTGCAAGCAATGCGTGTgg                                                                                                             <  1:2520324/150‑1 (MQ=255)
                                      gCCGCTGCGGGCCAGGTACTGACCATCATCGTTCGTACTATTACCGTTGCTTTCCAGCACGCTGCGGATAAGGCTGCTGATAACGGCAACCTGACAGCGATTTCCTGGAACCACGTTTCTTCTCTGTTCCTGCAAGCAATGCGTGTGGCt                                                                                                           >  2:2438347/1‑150 (MQ=255)
                                        cGCTGCGGGCCAGGTACTGACCATCATCGTTCGTACTATTACCGTTGCTTTCCAGCACGCTGCGGATAAGGCTGCTGATAACGGCAACCTGACAGCGATTTCCTGGAACCACGTTTCTTCTCTGTTCCTGCAAGCAATGCGTGTGGCTAt                                                                                                         <  2:2736570/150‑1 (MQ=255)
                                        cGCTGCGGGCCAGGTACTGACCATCATCGTTCGTACTATTACCGTTGCTTTCCAGCACGCTGCGGATAAGGCTGCTGATAACGGCAACCTGACAGCGATTTCCTGGAACCACGTTTCTTCTCTGTTCCTGCAAGCAATGCGTGTGGCTAt                                                                                                         <  2:1146431/150‑1 (MQ=255)
                                               ggCCAGGTACTGACCATCATCGTTCGTACTATTACCGTTGCTTTCCAGCACGCTGCGGATAAGGCTGCTGATAACGGCAACCTGACAGCGATTTCCTGGAACCACGTTTCTTCTCTGTTCCTGCAAGCAATGCGTGTGGCTATTCCGGcc                                                                                                  <  1:2101178/150‑1 (MQ=255)
                                               ggCCAGGTACTGACCATCATCGTTCGTACTATTACCGTTGCTTTCCAGCACGCTGCGGATAAGGCTGCTGATAACGGCAACCTGACAGCGATTTCCTGGAACCACGTTTCTTCTCTGTTCCTGCAAGCAATGCGTGTGGCTATTCCGGcc                                                                                                  >  2:3910174/1‑150 (MQ=255)
                                                   aGGTACTGACCATCATCGTTCGTACTATTACCGTTGCTTTCCAGCACGCTGCGGATAAGGCTGCTGATAACGGCAACCTGACAGCGATTTCCTGGAACCACGTTTCTTCTCTGTTCCTGCAAGCAATGCGTGTGGCTATTCCGGCCGTCa                                                                                              <  1:546336/150‑1 (MQ=255)
                                                       aCTGACCATCATCGTTCGTACTATTACCGTTGCTTTCCAGCACGCTGCGGATAAGGCTGCTGATAACGGCAACCTGACAGCGATTTCCTGGAACCACGTTTCTTCTCTGTTCCTGCAAGCAATGCGTGTGGCTATTCCGGCCGTCATCGt                                                                                          >  2:457436/1‑150 (MQ=255)
                                                              atcatcGTTCGTACTATTACCGTTGCTTTCCAGCACGCTGCGGATAAGGCTGCTGATAACGGCAACCTGACAGCGATTTCCTGGAACCACGTTTCTTCTCTGTTCCTGCAAGCAATGCGTGTGGCTATTCCGGCCGTCATCGTTGCGctg                                                                                   <  2:3861419/150‑1 (MQ=255)
                                                               tcatcGTTCGTACTATTACCGTTGCTTTCCAGCACGCTGCGGATAAGGCTGCTGATAACGGCAACCTGACAGCGATTTCCTGGAACCACGTTTCTTCTCTGTTCCTGCAAGCAATGCGTGTGGCTATTCCGGCCGTCATCGTTGCGctgt                                                                                  <  2:1322062/150‑1 (MQ=255)
                                                               tcatcGTTCGTACTATTACCGTTGCTTTCCAGCACGCTGCGGATAAGGCTGCTGATAACGGCAACCTGACAGCGATTTCCTGGAACCACGTTTCTTCTCTGTTCCTGCAAGCAATGCGTGTGGCTATTCCGGCCGTCATCGTTGCGctgt                                                                                  >  2:3493353/1‑150 (MQ=255)
                                                                     ttcgtACTATTACCGTTGCTTTCCAGCACGCTGCGGATAAGGCTGCTGATAACGGCAACCTGACAGCGATTTCCTGGAACCACGTTTCTTCTCTGTTCCTGCAAGCAATGCGTGTGGCTATTCCGGCCGTCATCGTTGCGCTGTCTGTTg                                                                            <  2:2827723/150‑1 (MQ=255)
                                                                     ttcgtACTATTACCGTTGCTTTCCAGCACGCTGCGGATAAGGCTGCTGATAACGGCAACCTGACAGCGATTTCCTGGAACCACGTTTCTTCTCTGTTCCTGCAAGCAATGCGTGTGGCTATTCCGGCCGTCATCGTTGCGCTGTCTGTTg                                                                            <  2:3717460/150‑1 (MQ=255)
                                                                      tcgtACTATTACCGTTGCTTTCCAGCACGCTGCGGATAAGGCTGCTGATAACGGCAACCTGACAGCGATTTCCTGGAACCACGTTTCTTCTCTGTTCCTGCAAGCAATGCGTGTGGCTATTCCGGCCGTCATCGTTGCGCTGTCTGTTgg                                                                           <  2:2802449/150‑1 (MQ=255)
                                                                       cgtACTATTACCGTTGCTTTCCAGCACGCTGCGGATAAGGCTGCTGATAACGGCAACCTGACAGCGATTTCCTGGAACCACGTTTCTTCTCTGTTCCTGCAAGCAATGCGTGTGGCTATTCCGGCCGTCATCGTTGCGCTGTCTGTTGGt                                                                          <  2:4041309/150‑1 (MQ=255)
                                                                         tACTATTACCGTTGCTTTCCAGCACGCTGCGGATAAGGCTGCTGATAACGGCAACCTGACAGCGATTTCCTGGAACCACGTTTCTTCTCTGTTCCTGCAAGCAATGCGTGTGGCTATTCCGGCCGTCATCGTTGCGCTGTCTGTTGGTAc                                                                        <  1:3012818/150‑1 (MQ=255)
                                                                          aCTATTACCGTTGCTTTCCAGCACGCTGCGGATAAGGCTGCTGATAACGGCAACCTGACAGCGATTTCCTGGAACCACGTTTCTTCTCTGTTCCTGCAAGCAATGCGTGTGGCTATTCCGGCCGTCATCGTTGCGCTGTCTGTTGGTAcc                                                                       <  2:2584639/150‑1 (MQ=255)
                                                                           cTATTACCGTTGCTTTCCAGCACGCTGCGGATAAGGCTGCTGATAACGGCAACCTGACAGCGATTTCCTGGAACCACGTTTCTTCTCTGTTCCTGCAAGCAATGCGTGTGGCTATTCCGGCCGTCATCGTTGCGCTGTCTGTTGGTACCa                                                                      <  2:731202/150‑1 (MQ=255)
                                                                             aTTACCGTTGCTTTCCAGCACGCTGCGGATAAGGCTGCTGATAACGGCAACCTGACAGCGATTTCCTGGAACCACGTTTCTTCTCTGTTCCTGCAAGCAATGCGTGTGGCTATTCCGGCCGTCATCGTTGCGCTGTCTGTTGGTACCAGc                                                                    <  1:747866/150‑1 (MQ=255)
                                                                             aTTACCGTTGCTTTCCAGCACGCTGCGGATAAGGCTGCTGATAACGGCAACCTGACAGCGATTTCCTGGAACCACGTTTCTTCTCTGTTCCTGCAAGCAATGCGTGTGGCTATTCCGGCCGTCATCGTTGCGCTGTCTGTTGGTACCAGc                                                                    <  1:3607234/150‑1 (MQ=255)
                                                                                  cGTTGCTTTCCAGCACGCTGCGGATAAGGCTGCTGATAACGGCAACCTGACAGCGATTTCCTGGAACCACGTTTCTTCTCTGTTCCTGCAAGCAATGCGTGTGGCTATTCCGGCCGTCATCGTTGCGCTGTCTGTTGGTACCAGCGAAGt                                                               <  2:1575646/150‑1 (MQ=255)
                                                                                   gTTGCTTTCCAGCACGCTGCGGATAAGGCTGCTGATAACGGCAACCTGACAGCGATTTCCTGGAACCACGTTTCTTCTCTGTTCCTGCAAGCAATGCGTGTGGCTATTCCGGCCGTCATCGTTGCGCTGTCTGTTGGTACCAGCGAAGTa                                                              >  2:1770585/1‑150 (MQ=255)
                                                                                     tGCTTTCCAGCACGCTGCGGATAAGGCTGCTGATAACGGCAACCTGACAGCGATTTCCTGGAACCACGTTTCTTCTCTGTTCCTGCAAGCAATGCGTGTGGCTATTCCGGCCGTCATCGTTGCGCTGTCTGTTGGTACCAGCGAAGTACa                                                            <  2:2884120/150‑1 (MQ=255)
                                                                                       cTTTCCAGCACGCTGCGGATAAGGCTGCTGATAACGGCAACCTGACAGCGATTTCCTGGAACCACGTTTCTTCTCTGTTCCTGCAAGCAATGCGTGTGGCTATTCCGGCCGTCATCGTTGCGCTGTCTGTTGGTACCAGCGAAGTACAGa                                                          <  1:2878161/150‑1 (MQ=255)
                                                                                       cTTTCCAGCACGCTGCGGATAAGGCTGCTGATAACGGCAACCTGACAGCGATTTCCTGGAACCACGTTTCTTCTCTGTTCCTGCAAGCAATGCGTGTGGCTATTCCGGCCGTCATCGTTGCGCTGTCTGTTGGTACCAGCGAAGTACAGa                                                          >  2:2078172/1‑150 (MQ=255)
                                                                                       cTTTCCAGCACGCTGCGGATAAGGCTGCTGATAACGGCAACCTGACAGCGATTTCCTGGAACCACGTTTCTTCTCTGTTCCTGCAAGCAATGCGTGTGGCTATTCCGGCCGTCATCGTTGCGCTGTCTGTTGGTACCAGCGAAGTACAGa                                                          <  1:46361/150‑1 (MQ=255)
                                                                                          tCCAGCACGCTGCGGATAAGGCTGCTGATAACGGCAACCTGACAGCGATTTCCTGGAACCACGTTTCTTCTCTGTTCCTGCAAGCAATGCGTGTGGCTATTCCGGCCGTCATCGTTGCGCTGTCTGTTGGTACCAGCGAAGTACAGAACa                                                       >  2:3976229/1‑150 (MQ=255)
                                                                                            cAGCACGCTGCGGATAAGGCTGCTGATAACGGCAACCTGACAGCGATTTCCTGGAACCACGTTTCTTCTCTGTTCCTGCAAGCAATGCGTGTGGCTATTCCGGCCGTCATCGTTGCGCTGTCTGTTGGTACCAGCGAAGTACAGAACATg                                                     >  2:3021388/1‑150 (MQ=255)
                                                                                               cACGCTGCGGATAAGGCTGCTGATAACGGCAACCTGACAGCGATTTCCTGGAACCACGTTTCTTCTCTGTTCCTGCAAGCAATGCGTGTGGCTATTCCGGCCGTCATCGTTGCGCTGTCTGTTGGTACCAGCGCAGTACAGAACATGCTg                                                  >  2:2049723/1‑150 (MQ=255)
                                                                                                aCGCTGCGGATAAGGCTGCTGATAACGGCAACCTGACAGCGATTTCCTGGAACCACGTTTCTTCTCTGTTCCTGCAAGCAATGCGTGTGGCTATTCCGGCCGTCATCGTTGCGCTGTCTGTTGGTACCAGCGAAGTACAGAACATGCTGa                                                 <  1:2637381/150‑1 (MQ=255)
                                                                                                 cGCTGCGGATAAGGCTGCTGATAACGGCAACCTGACAGCGATTTCCTGGAACCACGTTTCTTCTCTGTTCCTGCAAGCTATGCGTGTGGCTATTCCGGCCGTCATCGTTGCGCTGTCTGTTGGTACCAGCGAAGTACAGAACATGCTGaa                                                >  2:3284264/1‑150 (MQ=255)
                                                                                                 cGCTGCGGATAAGGCTGCTGATAACGGCAACCTGACAGCGATTTCCTGGAACCACGTTTCTTCTCTGTTCCTGCAAGCAATGCGTGTGGCTATTCCGGCCGTCATCGTTGCGCTGTCTGTTGGTACCAGCGAAGTACAGAACATGCTGaa                                                <  2:1072993/150‑1 (MQ=255)
                                                                                                  gCTGCGGATAAGGCTGCTGATAACGGCAACCTGACAGCGATTTCCTGGAACCACGTTTCTTCTCTGTTCCTGCAAGCAATGCGTGTGGCTATTTCGGCCGTCATCGTTGCGCTGTCTGTTGGTACCAGCGAAGTACAGAACATGCTGAAt                                               <  2:3134310/150‑1 (MQ=255)
                                                                                                  gCTGCGGATAAGGCTGCTGATAACGGCAACCTGACAGCGATTTCCTGGAACCACGTTTCTTCTCTGTTCCTGCAAGCAATGCGTGTGGCTATTCCGGCCGTCATCGTTGCGCTGTCTGTTGGTACCAGCGAAGTACAGAACATGCTGAAt                                               <  2:892611/150‑1 (MQ=255)
                                                                                                  gCTGCGGATAAGGCTGCTGATAACGGCAACCTGACAGCGATTTCCTGGAACCACGTTTCTTCTCTGTTCCTGCAAGCAATGCGTGTGGCTATTCCGGCCGTCATCGTTGCGCTGTCTGTTGGTACCAGCGAAGTACAGAACATGCTGAAt                                               >  1:324579/1‑150 (MQ=255)
                                                                                                     gCGGATAAGGCTGCTGATAACGGCAACCTGACAGCGATTTCCTGGAACCACGTTTCTTCTCTGTTCCTGCAAGCAATGCGTGTGGCTATTCCGGCCGTCATCGTTGCGCTGTCTGTTGGTACCAGCGAAGTACAGAACATGCTGAATGCg                                            <  2:3572227/150‑1 (MQ=255)
                                                                                                        gATAAGGCCGCTGATAACGGCAACCTGACAGCGATTTCCTGGAACCACGTTTCTTCTCTGTTCCTGCAAGCAATGCGTGTGGCTATTCCGGCCGTCATCGTTGCGCTGTCTGTTGGTACCAGCGAAGTACAGAACATGCTGAATGCGAtt                                         <  1:1556414/150‑1 (MQ=255)
                                                                                                          tAAGGCTGCTGATAACGGCAACCTGACAGCGATTTCCTGGAACCACGTTTCTTCTCTGTTCCTGCAAGCAATGCGTGTGGCTATTCCGGCCGTCATCGTTGCGCTGTCTGTTGGTACCAGCGAAGTACAGAACATGCTGAATGCGATTcc                                       >  2:250924/1‑150 (MQ=255)
                                                                                                          tAAGGCTGCTGATAACGGCAACCTGACAGCGATTTCCTGGAACCACGTTTCTTCTCTGTTCCTGCAAGCAATGCGTGTGGCTATTCCGGCCGTCATCGTTGCGCTGTCTGTTGGTACCAGCGAAGTACAGAACATGCTGAATGCGATTcc                                       >  2:144852/1‑150 (MQ=255)
                                                                                                           aaGGCTGCTGATAACGGCAACCTGACAGCGATTTCCTGGAACCACGTTTCTTCTCTGTTCCTGCAAGCAATGCGTGTGGCTATTCCGGCCGTCATCGTTGCGCTGTCTGTTGGTACCAGCGAAGTACAGAACATGCTGAATGCGATTCCg                                      >  2:1883214/1‑150 (MQ=255)
                                                                                                           aaGGCTGCTGATAACGGCAACCTGACAGCGATTTCCTGGAACCACGTTTCTTCTCTGTTCCTGCAAGCAATGCGTGTGGCTATTCCGGCCGTCATCGTTGCGCTGTCTGTTGGTACCAGCGAAGTACAGAACATGCTGAATGCGATTCCg                                      >  2:1179919/1‑150 (MQ=255)
                                                                                                                 gctgATAACGGCAACCTGACAGCGATTTCCTGGAACCACGTTTCTTCTCTGTTCCTGCAAGCAATGCGTGTGGCTATTCCGGCCGTCATCGTTGCGCTGTCTGTTGGTACCAGCGAAGTACAGAACATGCTGAATGCGATTCCGGAAgtg                                <  1:1108037/150‑1 (MQ=255)
                                                                                                                 gctgATAACGGCAACCTGACAGCGATTTCCTGGAACCACGTTTCTTCTCTGTTCCTGCAAGCAATGCGTGTGGCTATTCCGGCCGTCATCGTTGCGCTGTCTGTTGGTACCAGCGAAGTACAGAACATGCTGAATGCGATTCCGGAAgtg                                <  1:2323960/150‑1 (MQ=255)
                                                                                                                    gATAACGGCAACCTGACAGCGATTTCCTGGAACCACGTTTCTTCTCTGTTCCTGCAAGCAATGCGTGTGGCTATTCCGGCCGTCATCGTTGCGCTGTCTGTTGGTACCAGCGAAGTACAGAACATGCTGAATGCGATTCCGGAAgtggtg                             <  1:3880653/150‑1 (MQ=255)
                                                                                                                      tAACGGCAACCTGACAGCGATTTCCTGGAACCACGTTTCTTCTCTGTTCCTGCAAGCAATGCGTGTGGCTATTCCGGCCGTCATCGTTGCGCTGTCTGTTGGTACCAGCGAAGTACAGAACATGCTGAATGCGATTCCGGAAGTGGTGAc                           <  1:1751112/150‑1 (MQ=255)
                                                                                                                      tAACGGCAACCTGACAGCGATTTCCTGGAACCACGTTTCTTCTCTGTTCCTGCAAGCAATGCGTGTGGCTATTCCGGCCGTCATCGTTGCGCTGTCTGTTGGTACCAGCGAAGTACAGAACATGCTGAATGCGATTCCGGAAGTGGTGAc                           <  1:1762811/150‑1 (MQ=255)
                                                                                                                          ggCAACCTGACAGCGATTTCCTGGAACCACGTTTCTTCTCTGTTCCTGCAAGCAATGCGTGTGGCTATTCCGGCCGTCATCGTTGCGCTGTCTGTTGGTACCAGCGAAGTAc                                                             <  1:3579746/112‑1 (MQ=255)
                                                                                                                          ggCAACCTGACAGCGATTTCCTGGAACCACGTTTCTTCTCTGTTCCTGCAAGCAATGCGTGTGGCTATTCCGGCCGTCATCGTTGCGCTGTCTGTTGGTACCAGCGAAGTACAGAACATGCTGAATGCGATTCCGGAAGTGGTGACCAAt                       <  2:1592686/150‑1 (MQ=255)
                                                                                                                           gCAACCTGACAGCGATTTCCTGGAACCACGTTTCTTCTCTGTTCCTGCAAGCAATGCGTGTGGCTATTCCGGCCGTCATCGTTGCGCTGTCTGTTGGTACCAGCGAAGTACAGAACATGCTGAATGCGATTCCGGAAGTGGTGACCAATg                      <  1:1600103/150‑1 (MQ=255)
                                                                                                                            cAACCTGACAGCGATTTCCTGGAACCACGTTTCTTCTCTGTTCCTGCAAGCAATGCGTGTGGCTATTCCGGCCGTCATCGTTGCGCTGTCTGTTGGTACCAGCGAAGTACAGAACATGCTGAATGCGATTCCGGAAGTGGTGACCAATgg                     >  2:2536337/1‑150 (MQ=255)
                                                                                                                             aaCCTGACAGCGATTTCCTGGAACCACGTTTCTTCTCTGTTCCTGCAAGCAATGCGTGTGGCTATTCCGGCCGTCATCGTTGCGCTGTCTGTTGGTACCAGCGAAGTACAGAACATGCTGAATGCGATTCCGGAAGTGGTGACCAATGGt                    <  1:2487823/150‑1 (MQ=255)
                                                                                                                              aCCTGACAGCGATTTCCTGGAACCACGTTTCTTCTCTGTTCCTGCAAGCAATGCGTGTGGCTATTCCGGCCGTCATCGTTGCGCTGTCTGTTGGTACCAGCGAAGTACAGAACATGCTGAATGCGATTCCGGAAGTGGTGACCAATGGTc                   >  2:2175395/1‑150 (MQ=255)
                                                                                                                               ccTGACAGCGATTTCCTGGAACCACGTTTCTTCTCTGTTCCTGCAAGCAATGCGTGTGGCTATTCCGGCCGTCATCGTTGCGCTGTCTGTTGGTACCAGCGAAGTACAGAACATGCTGAATGCGATTCCGGAAGTGGTGACCAATGGTCt                  >  2:6115/1‑150 (MQ=255)
                                                                                                                                cTGACAGCGATTTCCTGGAACCACGTTTCTTCTCTGTTCCTGCAAGCAATGCGTGTGGCTATTCCGGCCGTCATCGTTGCGCTGTCTGTTGGTACCAGCGAAGTACAGAACATGCTGAATGCGATTCCGGAAGTGGTGACCAATGGTCTg                 >  1:3665474/1‑150 (MQ=255)
                                                                                                                                cTGACAGCGATTTCCTGGAACCACGTTTCTTCTCTGTTCCTGCAAGCAATGCGTGTGGCTATTCCGGCCGTCATCGTTGCGCTGTCTGTTGGTACCAGCGAAGTACAGAACATGCTGAATGCGATTCCGGAAGTGGTGACCAATGGTCTg                 <  1:1491245/150‑1 (MQ=255)
                                                                                                                                cTGACAGCGATTTCCTGGAACCACGTTTCTTCTCTGTTCCTGCAAGCAATGCGTGTGGCTATTCCGGCCGTCATCGTTGCGCTGTCTGTTGGTACCAGCGAAGTACAGAACATGCTGAATGCGATTCCGGAAGTGGTGACCAATGGTCTg                 >  2:315583/1‑150 (MQ=255)
                                                                                                                                 tGACAGCGATTTCCTGGAACCACGTTTCTTCTCTGTTCCTGCAAGCAATGCGTGTGGCTATTCCGGCCGTCATCGTTGCGCTGTCTGTTGGTACCAGCGAAGTACAGAACATGCTGAATGCGATTCCGGAAGTGGTGACCAATGGTCTGa                <  1:3714485/150‑1 (MQ=255)
                                                                                                                                  gACAGCGATTTCCTGGAACCACGTTTCTTCTCTGTTCCTGCAAGCAATGCGTGTGGCTATTCCGGCCGTCATCGTTGCGCTGTCTGTTGGTACCAGCGAAGTACAGAACATGCTGAATGCGATTCCGGAAGTGGTGACCAATGGTCTGaa               <  1:914595/150‑1 (MQ=255)
                                                                                                                                  gACAGCGATTTCCTGGAACCACGTTTCTTCTCTGTTCCTGCAAGCAATGCGTGTGGCTATTCCGGCCGTCATCGTTGCGCTGTCTGTTGGTACCAGCGAAGTACAGAACATGCTGAATGCGATTCCGGAAGTGGTGACCAATGGTCTGaa               <  2:222484/150‑1 (MQ=255)
                                                                                                                                   aCAGCGATTTCCTGGAACCACGTTTCTTCTCTGTTCCTGCAAGCAATGCGTGTGGCTATTCCGGCCGTCATCGTTGCGCTGTCTGTTGGTACCAGCGAAGTACAGAACATGCTGAATGCGATTCCGGAAGTGGTGACCAATGGTCTGAat              <  2:3690160/150‑1 (MQ=255)
                                                                                                                                   aCAGCGATTTCCTGGAACCACGTTTCTTCTCTGTTCCTGCAAGCAATGCGTGTGGCTATTCCGGCCGTCATCGTTGCGCTGTCTGTTGGTACCAGCGAAGTACAGAACATGCTGAATGCGATTCCGGAAGTGGTGACCAATGGTCTGAat              <  2:57798/150‑1 (MQ=255)
                                                                                                                                      gCGATTTCCTGGAACCACGTTTCTTCTCTGTTCCTGCAAGCAATGCGTGTGGCTATTCCGGCCGTCATCGTTGCGCTGTCTGTTGGTACCAGCGAAGTACAGAACATGCTGAATGCGATTCCGGAAGTGGTGACCAATGGTCTGAATATc           <  1:296856/150‑1 (MQ=255)
                                                                                                                                         aTTTCCTGGAACCACGTTTCTTCTCTGTTCCTGCAAGCAATGCGTGTGGCTATTCCGGCCGTCATCGTTGCGCTGTCTGTTGGTACCAGCGAAGTACAGAACATGCTGAATGCGATTCCGGAAGTGGTGACCAATGGTCTGAATATCGcc        <  1:3733922/150‑1 (MQ=255)
                                                                                                                                           ttCCTGGAACCACGTTTCTTCTCTGTTCCTGCAAGCAATGCGTGTGGCTATTCCGGCCGTCATCGTTGCGCTGTCTGTTGGTACCAGCGAAGTACAGAACATGCTGAATGCGATTCCGGAAGTGGTGACCAATGGTCTGAATATCGCCgg      >  1:2892234/1‑150 (MQ=255)
                                                                                                                                             ccTGGAACCACGTTTCTTCTCTGTTCCTGCAAGCAATGCGTGTGGCTATTCCGGCCGTCATCGTTGCGCTGTCTGTTGGTACCAGCGAAGTACAGAACATGCTGAATGCGATTCCGGAAGTGGTGACCAATGGTCTGAATATCGCCGGTg    >  1:3401599/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
AGAGCATTGGTGCAGGTATCGCACTGGCAATCCCTCTGGCCGCTGCGGGCCAGGTACTGACCATCATCGTTCGTACTATTACCGTTGCTTTCCAGCACGCTGCGGATAAGGCTGCTGATAACGGCAACCTGACAGCGATTTCCTGGATCCACGTTTCTTCTCTGTTCCTGCAAGCAATGCGTGTGGCTATTCCGGCCGTCATCGTTGCGCTGTCTGTTGGTACCAGCGAAGTACAGAACATGCTGAATGCGATTCCGGAAGTGGTGACCAATGGTCTGAATATCGCCGGTGGC  >  AP012306/1583496‑1583788

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑