BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AP012306	1,591,969	G→A	Q252* (CAA→TAA)	htpX ←	predicted endopeptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AP012306	1,591,969	0	G	A	100.0%	952.7 / NA	236	Q252* (CAA→TAA)	htpX	predicted endopeptidase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (79/157); total (79/157)

Only 100 of 258 total aligned reads displayed.

TTTTAGTATTTACTTCATATTACTTCAGGTATTCACCCGTACGCAGAGCTTCAATTCGTTTATCCAGCGGCGGGTGGGTCATGAACAACTCACTGAGCGATTTCGACTTACCGTTAATGCAGAGAGCCATCATGCTGGTTGCTTCTTGCGGTTCATAGCTGGTTTTCAGGCGCTGCAGCGCGGCAATCATTTTCTCGCGACCAACCAGTTTTGCCGAACCGGCATCAGCATGGAATTCACGATGACGCGAGAACCACATGGTGATAATGCTCGCCAGAATACCAAACACCAGTT  >  AP012306/1591822‑1592115

  ttAGTATTTACTTCATATTACTTCAGGTATTCACCCGTACGCAGAGCTTCAATTCGTTTATCCAGCGGCGGGTGGGTCATGAACAACTCACTGAGCGATTTCGACTTACCGTTAATGCAGAGAGCCATCATGCTGGTTGCTTCTTACGGt                                                                                                                                                <  1:584990/150‑1 (MQ=255)

   tAGTATTTACTTCATATTACTTCAGGTATTCACCCGTACGCAGAGCTTCAATTCGTTTATCCAGCGGCGGGTGGGTCATGAACAACTCACTGAGCGATTTCGACTTACCGTTAATGCAGAGAGCCATCATGCTGGTTGCTTCTTACGGtt                                                                                                                                               <  1:1002707/150‑1 (MQ=255)

   tAGTATTTACTTCATATTACTTCAGGTATTCACCCGTACGCAGAGCTTCAATTCGTTTATCCAGCGGCGGGTGGGTCATGAACAACTCACTGAGCGATTTCGACTTACCGTTAATGCAGAGAGCCATCATGCTGGTTGCTTCTTACGGtt                                                                                                                                               <  2:3939715/150‑1 (MQ=255)

     gTATTTACTTCATATTACTTCAGGTATTCACCCGTACGCAGAGCTTCAATTCGTTTATCCAGCGGCGGGTGGGTCATGAACAACTCACTGAGCGATTTCGACTTACCGTTAATGCAGAGAGCCATCATGCTGGTTGCTTCTTACGGTTCa                                                                                                                                             <  1:3346741/150‑1 (MQ=255)

      tATTTACTTCATATTACTTCAGGTATTCACCCGTACGCAGAGCTTCAATTCGTTTATCCAGCGGCGGGTGGGTCATGAACAACTCACTGAGCGATTTCGACTTACCGTTAATGCAGAGAGCCATCATGCTGGTTGCTTCTTACGGTTCAt                                                                                                                                            <  2:3208569/150‑1 (MQ=255)

       aTTTACTTCATATTACTTCAGGTATTCACCCGTACGCAGAGCTTCAATTCGTTTATCCAGCGGCGGGTGGGGCATGAACAAAACACTTAGCGGTTTTTACTTTCCCTTTATGCAGAGAGGCATCATTCTGGTTGCTTTTTTTGGTTtata                                                                                                                                           >  2:2197931/1‑150 (MQ=255)

             ttCATATTACTTCAGGTATTCACCCGTACGCAGAGCTTCAATTCGTTTATCCAGCGGCGGGTGGGTCATGAACAACTCACTGAGCGATTTCGACTTACCGTTAATGCAGAGAGCCATCATGCTGGTTGCTTCTTACGGTTCATAGCTGGt                                                                                                                                     <  2:3796669/150‑1 (MQ=255)

             ttCATATTACTTCAGGTATTCACCCGTACGCAGAGCTTCAATTCGTTTATCCAGCGGCGGGTGGGTCATGAACAACTCACTGAGCGATTTCGACTTACCGTTAATGCAGAGAGCCATCATGCTGGTTGCTTCTTACGGTTCATAGCTGGt                                                                                                                                     <  1:2158017/150‑1 (MQ=255)

              tCATATTACTTCAGGTATTCACCCGTACGCAGAGCTTCAATTCGTTTATCCAGCGGCGGGTGGGTCATGAACAACTCACTGAGCGATTTCGACTTACCGTTAATGCAGAGAGCCATCATGCTGGTTGCTTCTTACGGTTCATAGCTGGtt                                                                                                                                    <  1:3959443/150‑1 (MQ=255)

               cATATTACTTCAGGTATTCACCCGTACGCAGAGCTTCAATTCGTTTATCCAGCGGCGGGTGGGTCATGAACAACTCACTGAGCGATTTCGACTTACCGTTAATGCAGAGAGCCATCATGCTGGTTGCTTCTTACGGTTCATAGCTGGttt                                                                                                                                   <  2:1887149/150‑1 (MQ=255)

               cATATTACTTCAGGTATTCACCCGTACGCAGAGCTTCAATTCGTTTATCCAGCGGCGGGTGGGTCATGAACAACTCACTGAGCGATTTCGACTTACCGTTAATGCAGAGAGCCATCATGCTGGTTGCTTCTTACGGTTCATAGCTGGttt                                                                                                                                   <  1:1786859/150‑1 (MQ=255)

                   ttACTTCAGGTATTCACCCGTACGCAGAGCTTCAATTCGTTTATCCAGCGGCGGGTGGGTCATGAACAACTCACTGAGCGATTTCGACTTACCGTTAATGCAGAGAGCCATCATGCTGGTTGCTTCTTACGGTTCATAGCTGGTTTTCAg                                                                                                                               <  2:2798959/150‑1 (MQ=255)

                        tCAGGTATTCGCCCGTACGCAGAGCTTCAATTCGTTTATCCAGCGGCGGGTGGGTCATGCACAACTCACTGAGCGATTTCGACTTACCGTTAATGCAGAGAGCCATCATGCTGGTTGCTTCTTACGGTTCATAGCTGGTTTTCAGGCGCt                                                                                                                          <  1:1025570/150‑1 (MQ=255)

                        tCAGGTATTCACCCGTACGCAGAGCTTCAATTCGTTTATCCAGCGGCGGGTGGGTCATGAACAACTCACTGAGCGATTTCGACTTACCGTTAATGCAGAGAGCCATCATGCTGGTTGCTTCTTACGGTTCATAGCTGGTTTTCAGGCGCt                                                                                                                          <  2:1368177/150‑1 (MQ=255)

                        tCAGGTATTCACCCGTACGCAGAGCTTCAATTCGTTTATCCAGCGGCGGGTGGGTCATGAACAACTCACTGAGCGATTTCGACTTACCGTTAATGCAGAGAGCCATCATGCTGGTTGCTTCTTACGGTTCATAGCTGGTTTTCAGGCGCt                                                                                                                          <  2:440819/150‑1 (MQ=255)

                           ggTATTCACCCGTACGCAGAGCTTCAATTCGTTTATCCAGCGGCGGGTGGGTCATGAACAACTCACTGAGCGATTTCGACTTACCGTTAATGCAGAGAGCCATCATGCTGGTTGCTTCTTACGGTTCATAGCTGGTTTTCAGGCGCTGCa                                                                                                                       <  1:3428358/150‑1 (MQ=255)

                            gTATTCACCCGTACGCAGAGCTTCAATTCGTTTATCCAGCGGCGGGGGGGGCATGAACAAAACACTGAGCGGTTTTGACTTACCGTTAATGCAGAGAGGCATCATGCTGGGTGCTTTTTTCGGTTCATAGCTGGGTTTTAGGCGCTGCAg                                                                                                                      >  2:699818/1‑150 (MQ=255)

                              aTTCACCCGTACGCAGAGCTTCAATTCGTTTATCCAGCGGCGGGGGGGGCATGAACAACTCACTCAGCGGTTTTGACTTTCCGTTAATGCAGAGAGCCATCATGCTGGGTGGTTCTTTCGGGTCATAACTGGGTTTTAGGCGCTGCAgcg                                                                                                                    >  2:3667018/1‑150 (MQ=255)

                               ttCACCCGTACGCAGAGCTTCAATTCGTTTATCCAGCGGCGGGGGGGGGATGAACAACTCACTTAGCGACTTTTACTTACCGTTAATGCAGAGAGGCATCATGCTGGTTGCTTCTTACGGTTCTTAGCTCGGTTTTAGGCGCGGCAgcgc                                                                                                                   >  2:2678072/1‑150 (MQ=255)

                                   cccGTACGCAGAGCTTCAATTCGTTTATCCAGCGGCGGGGGGGGCGTGAACAAAACACTGAGCGATTTTGACTTTCCCTTAATGCAGAGGGCCATCATGCTGGTTGCTTCTTTCGGTTTCTAGCTGGGTTTTTGGCGCCGCAGCGCGGcc                                                                                                               >  2:1821963/1‑149 (MQ=255)

                                   cccGTACGCAGAGCTTCAATTCGTTTATCCAGCGGCGGGGGGGGCATGAAAAAATCACTGAGCGGTTTTTACTTTCCCGTTATGCAGAGAGCCCTCATGCTGGGTGGTTTTTTCGGTTCCTAGCTGGGTTTTCGGCGCCGCCGCGCGGCa                                                                                                               >  2:3470056/1‑150 (MQ=255)

                                     cGTACGCAGAGCTTCAATTCGTTTATCCAGCGGCGGGTGGGTCATGAACAACTCACTGAGCGATTTCGACTTACCGTTAATGCAGAGAGCCATCATGCTGGTTGCTTCTTACGGTTCATAGCTGGTTTTCAGGCGCTGCAGCGCGGCAAt                                                                                                             <  2:1989450/150‑1 (MQ=255)

                                         cGCAGAGCTTCAATTCGTTTATCCAGCGGCGGGTGGGTCATGAACAACTCACTGAGCGATTTCGACTTACCGTTAATGCAGAGAGCCATCATGCTGGTTGCTTCTTACGGTTCATAGCTGGTTTTCAGGCGCTGCAGCGCGGCAATCAtt                                                                                                         <  1:1985287/150‑1 (MQ=255)

                                             gagCTTCAATTCGTTTATCCAGCGGCGGGTGGGTCATGAACAACTCACTGAGCGATTTCGACTTACCGTTAATGCAGAGAGCCATCATGCTGGTTGCTTCTTACGGTTCATAGCTGGTTTTCAGGCGCTGCAGCGCGGCAATCATTTtct                                                                                                     <  1:3094655/150‑1 (MQ=255)

                                              agCTTCAATTCGTTTATCCAGCGGCGGGTGGGTCATGAACAACTCACTGAGCGATTTCGACTTACCGTTAATGCAGAGAGCCATCATGCTGGTTGCTTCTTACGGTTCATAGCTGGTTTTCAGGCGCTGCAGCGCGGCAATCATTTtctc                                                                                                    <  1:2613534/150‑1 (MQ=255)

                                              agCTTCAATTCGTTTATCCAGCGGCGGGTGGGTCATGAACAACTCACTGAGCGATTTCGACTTACCGTTAATGCAGAGAGCCATCATGCTGGTTGCTTCTTACGGTTCATAGCTGGTTTTCAGGCGCTGCAGCGCGGCAATCATTTtctc                                                                                                    <  1:3879640/150‑1 (MQ=255)

                                               gCTTCAATTCGTTTATCCAGCGGCGGGGGGGGCATGAAAAAACCACTTAGCGATTTTTACTTTCCCTTTATGCAGAGAGCCCTCATGCTGGGTTCTTCTTTCGGTTTATAGCTGGGTTTTAGGCGCTGCCGCGCGGCAATCCTTTTTTcg                                                                                                   >  2:1218871/1‑150 (MQ=255)

                                                cTTCAATTCGTTTATCCAGCGGCGGGTGGGTCATGAACAACTCACTGAGCGATTTCGACTTACCGTTAATGCAGAGAGCCATCATGCTGGTTGCTTCTTACGGTTCATAGCTGGTTTTCAGGCGCTGCAGCGCGGCAATCATTTTCTcgc                                                                                                  <  2:1622296/150‑1 (MQ=255)

                                                cTTCAATTCGTTTATCCAGCGGCGGGTGGGTCATGAACAACTCACTGAGCGATTTCGACTTACCGTTAATGCAGAGAGCCATCATGCTGGTTGCTTCTTACGGTTCATAGCTGGTTTTCAGGCGCTGCAGCGCGGCAATCATTTTCTcgc                                                                                                  <  2:1100797/150‑1 (MQ=255)

                                                 ttCAATTCGTTTATCCAGCGGCGGGTGGGTCATGAACAACTCACTGAGCGATTTCGACTTACCGTTAATGCAGAGAGCCATCATGCTGGTTGCTTCTTACGGTTCATAGCTGGTTTTCAGGCGCTGCAGCGCGGCAATCATTTTCTcgcg                                                                                                 <  2:1493269/150‑1 (MQ=255)

                                                    aaTTCGTTTATCCAGCGGCGGGGGGGGGATGAAAAAAACACTTAGCGGGTTTGGCTTAACGTTAATGGAGAGAGGCATCATGCTGGGTGCTTCTTTCGGTTTATAACTGGGTTTTTTGCGCGGCAGCGCGGCGAACAATTTTTCTCGacc                                                                                              >  2:4113640/1‑150 (MQ=255)

                                                      ttCGTTTATCCAGCGGCGGGTGGGTCATGAACAACTCACTGAGCGATTTCGACTTACCGTTAATGCAGAGAGCCATCATGCTGGTTGCTTCTTACGGTTCATAGCTGGTTTTCAGGCGCTGCAGCGCGGCAATCATTTTCTCGCGaccaa                                                                                            <  2:2589334/150‑1 (MQ=255)

                                                        cGTTTATCCAGCGGCGGGTGGGTCATGAACAACTCACTGAGCGATTTCGACTTACCGTTAATGCAGAGAGCCATCATGCTGGTTGCTTCTTACGGTTCATAGCTGGTTTTCAGGCGCTGCAGCGCGGCAATCATTTTCTCGCGaccaacc                                                                                          <  2:1756568/150‑1 (MQ=255)

                                                        cGTTTATCCAGCGGCGGGGGGGGCATGGAAAAATCACTGGGCGGTTTTTACTTTCCCTTTATGCAGAGAGCCATCATGCTGGTTGCTTTTTTCGGTTTATAGCTGGGTTTTATGCGCTGCAGCGCGGGGATCATTTTTTCGCGacccaac                                                                                          >  2:940110/1‑146 (MQ=255)

                                                          tttATCCAGCGGCGGGTGGGTCATGAACAACTCACTGAGCGATTTCGACTTACCGTTAATGCAGAGAGCCATCATGCTGGTTGCTTCTTACGGTTCATAGCTGGTTTTCAGGCGCTGCAGCGCGGCAATCATTTTCTCGCGACCAACCAg                                                                                        <  2:497217/150‑1 (MQ=255)

                                                            tATCCAGCGGCGGGTGGGTCATGAACAACTCACTGAGCGATTTCGACTTACCGTTAATGCAGAGAGCCATCATGCTGGTTGCTTCTTACGGTTCATAGCTGGTTTTCAGGCGCTGCAGCGCGGCAATCATTTTCTCGCGACCAACCAGtt                                                                                      <  2:1281517/150‑1 (MQ=255)

                                                             aTCCAGCGGCGGGTGGGTCATGAACAACTCACTGAGCGATTTCGACTTACCGTTAATGCAGAGAGCCATCATGCTGGTTGCTTCTTACGGTTCATAGCTGGTTTTCAGGCGCTTCAGCGCGGCAATCATTTTCTCGCGACCAACCAGttt                                                                                     <  2:3286931/150‑1 (MQ=255)

                                                               ccAGCGGCGGGTGGGTCATGAACAACTCACTGAGCGATTTCGACTTACCGTTAATGCAGAGAGCCATCACGCTGGTTGCTTCTTACGGTTCATAGCTGGTTTTCAGGCGCTGCAGCGCGGCAATCATTTTCTCGCGACCAACCAGTTTTg                                                                                   <  1:658658/150‑1 (MQ=255)

                                                                cAGCGGCGGGTGGGTCATGAACAACTCACTGAGCGATTTCGACTTACCGTTAATGCAGAGAGCCATCATGCTGGTTGCTTCTTACGGTTCATAGCTGGTTTTCAGGCGCTGCAGCGCGGCAATCATTTTCTCGCGACCAACCAGTTTTGc                                                                                  <  1:286358/150‑1 (MQ=255)

                                                                  gcggcgGGTGGGTCATGAACAACTCACTGAGCGATTTCGACTTACCGTTAATGCAGAGAGCCATCATGCTGGTTGCTTCTTACGGTTCATAGCTGGTTTTCAGGCGCTGCAGCGCGGCAATCATTTTCTCGCGACCAACCAGTTTTGCCg                                                                                <  2:3865183/150‑1 (MQ=255)

                                                                  gcggcgGGTGGGTCATGAACAACTCACTGAGCGATTTCGACTTACCGTTAATGCAGAGAGCCATCATGCTGGTTGCTTCTTACGGTTCATAGCTGGTTTTCAGGCGCTGCAGCGCGGCAATCATTTTCTCGCGACCAACCAGTTTTGCCg                                                                                <  1:1131470/150‑1 (MQ=255)

                                                                       gggtgggtCATGAACAACTCACTGAGCGATTTCGACTTACCGTTAATGCAGAGAGCCATCATGCTGGTTGCTTCTTACGGTTCATAGCTGGTTTTCAGGCGCTGCAGCGCGGCAATCATTTTCTCGCGACCAACCAGTTTTGCCGAACCg                                                                           >  1:277826/1‑150 (MQ=255)

                                                                       gggtgggtCATGAACAACTCACTGAGCGATTTCGACTTACCGTTAATGCAGAGAGCCATCATGCTGGTTGCTTCTTACGGTTCATAGCTGGTTTTCAGGCGCTGCAGCGCGGCAATCATTTTCTCGCGACCAACCAGTTTTGCCGAACCg                                                                           >  2:2869065/1‑150 (MQ=255)

                                                                         gtgggtCATGAACAACTCACTGAGCGATTTCGACTTACCGTTAATGCAGAGAGCCATCATGCTGGTTGCTTCTTACGGTTCATAGCTGGTTTTCAGGCGCTGCAGCGCGGCAATCATTTTCTCGCGACCAACCAGTTTTGCCGAACCGGc                                                                         >  1:850922/1‑150 (MQ=255)

                                                                          tgggtCATGAACAACTCACTGAGCGATTTCGACTTACCGTTAATGCAGAGAGCCATCATGCTGGTTGCTTCTTACGGTTCATAGCTGGTTTTCAGGCGCTGCAGCGCGGCAATCATTTTCTCGCGACCAACCAGTTTTGCCGAACCGGCa                                                                        >  1:4034247/1‑150 (MQ=255)

                                                                              tCATGAACAACTCACTGAGCGATTTCGACTTACCGTTAATGCAGAGAGCCATCATGCTGGTTGCTTCTTACGGTTCATAGCTGGTTTTCAGGCGCTGCAGCGCGGCAATCATTTTCTCGCGACCAACCAGTTTTGCCGAACCGGCATCAg                                                                    >  2:761399/1‑150 (MQ=255)

                                                                                  gAACAACTCACTGAGCGATTTCGACTTACCGTTAATGCAGAGAGCCATCATGCTGGTTGCTTCTTACGGTTCATAGCTGGTTTTCAGGCGCTGCAGCGCGGCAATCATTTTCTCGCGACCAACCAGTTTTGCCGAACCGGCATCAGCATg                                                                >  2:1009620/1‑150 (MQ=255)

                                                                                         tCACTGAGCGATTTCGACTTACCGTTAATGCAGAGAGCCATCATGCTGGTTGCTTCTTACGGTTCATAGCTGGTTTTCAGGCGCTGCAGCGCGGCAATCATTTTCTCGCGACCAACCAGTTTTGCCGAACCGGCATCAGCATGGAATTCa                                                         <  2:2395719/150‑1 (MQ=255)

                                                                                            cTGAGCGATTTCGACTTACCGTTAATGCAGAGAGCCATCATGCTGGTTGCTTCTTACGGTTCATAGCTGGTTTTCAGGCGCTGCAGCGCGGCAATCATTTTCTCGCGACCAACCAGTTTTGCCGAACCGGCATCAGCATGGAATTCACGa                                                      >  1:348074/1‑150 (MQ=255)

                                                                                              gAGCGATTTCGACTTACCGTTAATGCAGAGAGCCATCATGCTGGTTGCTTCTTACGGTTCATAGCTGGTTTTCAGGCGCTGCAGCGCGGCAATCATTTTCTCGCGACCAACCAGTTTTGCCGAACCGGCATCAGCATGGAATTCACGATg                                                    >  1:1676009/1‑150 (MQ=255)

                                                                                              gAGCGATTTCGACTTACCGTTAATGCAGAGAGCCATCATGCTGGTTGCTTCTTACGGTTCATAGCTGGTTTTCAGGCGCTGCAGCGCGGCAATCATTTTCTCGCGACCAACCAGTTTTGCCGAACCGGCATCAGCATGGAATTCACGATg                                                    >  2:3776773/1‑150 (MQ=255)

                                                                                              gAGCGATTTCGACTTACCGTTAATGCAGAGAGCCATCATGCTGGTTGCTTCTTACGGTTCATAGCTGGTTTTCAGGCGCTGCAGCGCGGCAATCATTTTCTCGCGACCAACCAGTTTTGCCGAACCGGCATCAGCATGGAATTCACGATg                                                    <  1:2839734/150‑1 (MQ=255)

                                                                                               aGCGATTTCGACTTACCGTTAATGCAGAGAGCCATCATGCTGGTTGCTTCTTACGGTTCATAGCTGGTTTTCAGGCGCTGCAGCGCGGCAATCATTTTCTCGCGACCAACCAGTTTTGCCGAACCGGCATCAGCATGGAATTCACGATGa                                                   >  1:73905/1‑150 (MQ=255)

                                                                                               aGCGATTTCGACTTACCGTTAATGCAGAGAGCCATCATGCTGGTTGCTTCTTACGGTTCATAGCTGGTTTTCAGGCGCTGCAGCGCGGCAATCATTTTCTCGCGACCAACCAGTTTTGCCGAACCGGCATCAGCATGGAATTCACGATGa                                                   >  2:3899568/1‑150 (MQ=255)

                                                                                                 cGATTTCGACTTACCGTTAATGCAGAGAGCCATCATGCTGGTTGCTTCTTACGGTTCATAGCTGGTTTTCAGGCGCTGCAGCGCGGCAATCATTTTCTCGCGACCAACCAGTTTTGCCGAACCGGCATCAGCATGGAATTCACGATGAcg                                                 <  1:2730438/150‑1 (MQ=255)

                                                                                                 cGATTTCGACTTACCGTTAATGCAGAGAGCCATCATGCTGGTTGCTTCTTACGGTTCATAGCTGGTTTTCAGGCGCTGCAGCGCGGCAATCATTTTCTCGCGACCAACCAGTTTTGCCGAACCGGCATCAGCATGGAATTCACGATGAcg                                                 >  1:1523115/1‑150 (MQ=255)

                                                                                                  gATTTCGACTTACCGTTAATGCAGAGAGCCATCATGCTGGTTGCTTCTTACGGTTCATAGCTGGTTTTCAGGCGCTGCAGCGCGGCAATCATTTtct                                                                                                     <  1:1874808/97‑1 (MQ=255)

                                                                                                   aTTTCGACTTACCGTTAATGCAGAGAGCCATCATGCTGGTTGCTTCTTACGGTTCATAGCTGGTTTTCAGGCGCTGCAGCGCGGCAATCATTTTCTCGCGACCAACCAGTTTTGCCGAACCGGCATCAGCATGGAATTCACGATGAcgcg                                               >  1:245924/1‑150 (MQ=255)

                                                                                                   aTTTCGACTTACCGTTAATGCAGAGAGCCATCATGCTGGTTGCTTCTTACGGTTCATAGCTGGTTTTCAGGCGCTGCAGCGCGGCAATCATTTTCTCGCGACCAACCAGTTTTGCCGAACCGGCATCAGCATGGAATTCACGATGAcgcg                                               <  2:1541388/150‑1 (MQ=255)

                                                                                                      tCGACTTACCGTTAATGCAGAGAGCCATCATGCTGGTTGCTTCTTACGGTTCATAGCTGGTTTTCAGGCGCTGCAGCGCGGCAATCATTTTCTCGCGACCAACCAGTTTTGCCGAACCGGCATCAGCATGGAATTCACGATGACGCgaga                                            <  2:2097032/150‑1 (MQ=255)

                                                                                                        gACTTACCGTTAATGCAGAGAGCCATCATGCTGGTTGCTTCTTACGGTTCATAGCTGGTTTTCAGGCGCTGCAGCGCGGCAATCATTTTCTCGCGACCAACCAGTTTTGCCGAACCGGCATCAGCATGGAATTCACGATGACGCGAGAAc                                          >  2:2747189/1‑150 (MQ=255)

                                                                                                        gACTTACCGTTAATGCAGAGAGCCATCATGCTGGTTGCTTCTTACGGTTCATAGCTGGTTTTCAGGCGCTGCAGCGCGGCAATCATTTTCTCGCGACCAACCAGTTTTGCCGAACCGGCATCAGCATGGAATTCACGATGACGCGAGAAc                                          <  2:2950244/150‑1 (MQ=255)

                                                                                                         aCTTACCGTTAATGCAGAGAGCCATCATGCTGGTTGCTTCTTACGGTTCATAGCTGGTTTTCAGGCGCTGCAGCGCGGCAATCATTTTCTCGCGACCAACCAGTTTTGCCGAACCGGCATCAGCATGGAATTCACGATGACGCGAGAAcc                                         <  2:3354270/150‑1 (MQ=255)

                                                                                                          cTTACCGTTAATGCAGAGAGCCATCATGCTGGTTGCTTCTTACGGTTCATAGCTGGTTTTCAGGCGCTGCAGCGCGGCAATCATTTTCTCGCGACCAACCAGTTTTGCCGAACCGGCATCAGCATGGAATTCACGATGACGCGAGAACca                                        >  2:152329/1‑150 (MQ=255)

                                                                                                          cTTACCGTTAATGCAGAGAGCCATCATGCTGGTTGCTTCTTACGGTTCATAGCTGGTTTTCAGGCGCTGCAGCGCGGCAATCATTTTCTCGCGACCAACCAGTTTTGCCGAACCGGCATCAGCATGGAATTCACGATGACGCGAGAACca                                        >  1:750821/1‑150 (MQ=255)

                                                                                                          cTTACCGTTAATGCAGAGAGCCATCATGCTGGTTGCTTCTTACGGTTCATAGCTGGTTTTCAGGCGCTGCAGCGCGGCAATCATTTTCTCGCGACCAACCAGTTTTGCCGAACCGGCATCAGCATGGAATTCACGATGACGCGAGAACca                                        <  2:307438/150‑1 (MQ=255)

                                                                                                             aCCGTTAATGCAGAGAGCCATCATGCTGGTTGCTTCTTACGGTTCATAGCTGGTTTTCAGGCGCTGCAGCGCGGCAATCATTTTCTCGCGACCAACCAGTTTTGCCGAACCGGCATCAGCATGGAATTCACGATGACGCGAGAACCACAt                                     <  1:2264355/150‑1 (MQ=255)

                                                                                                              ccGTTAATGCAGAGAGCCATCATGCTGGTTGCTTCTTACGGTTCATAGCTGGTTTTCAGGCGCTGCAGCGCGGCAATCATTTTCTCGCGACCAACCAGTTTTGCCGAACCGGCATCAGCATGGAATTCACGATGACGCGAGAACCACATg                                    <  2:3892625/150‑1 (MQ=255)

                                                                                                               cGTTAATGCAGAGAGCCATCATGCTGGTTGCTTCTTACGGTTCATAGCTGGTTTTCAGGCGCTGCAGCGCGGCAATCATTTTCTCGCGACCAACCAGTTTTGCCGAACCGGCATCAGCATGGAATTCACGATGACGCGAGAACCACATgg                                   <  1:3667018/150‑1 (MQ=255)

                                                                                                                 ttAATGCAGAGAGCCATCATGCTGGTTGCTTCTTACGGTTCATAGCTGGTTTTCAGGCGCTGCAGCGCGGCAATCATTTTCTCGCGACCAACCAGTTTTGCCGAACCGGCATCAGCATGGAATTCACGATGACGCGAGAACCACATGGTg                                 <  1:1278680/150‑1 (MQ=255)

                                                                                                                  tAATGCAGAGAGCCATCATGCTGGTTGCTTCTTACGGTTCATAGCTGGTTTTCAGGCGCTGCAGCGCGGCAATCATTTTCTCGCGACCAACCAGTTTTGCCGAACCGGCATCAGCATGGAATTCACGATGACGCGAGAACCACATGGTGa                                <  2:516051/150‑1 (MQ=255)

                                                                                                                    aTGCAGAGAGCCATCATGCTGGTTGCTTCTTACGGTTCATAGCTGGTTTTCAGGCGCTGCAGCGCGGCAATCATTTTCTCGCGACCAACCAGTTTTGCCGAACCGGCATCAGCATGGAATTCACGATGACGCGAGAACCACATGGTGATa                              <  2:4027884/150‑1 (MQ=255)

                                                                                                                    aTGCAGAGAGCCATCATGCTGGTTGCTTCTTACGGTTCATAGCTGGTTTTCAGGCGCTGCAGCGCGGCAATCATTTTCTCGCGACCAACCAGTTTTGCCGAACCGGCATCAGCATGGAATTCACGATGACGCGAGAACCACATGGTGATa                              <  2:2337393/150‑1 (MQ=255)

                                                                                                                    aTGCAGAGAGCCATCATGCTGGTTGCTTCTTACGGTTCATAGCTGGTTTTCAGGCGCTGCAGCGCGGCAATCATTTTCTCGCGACCAACCAGTTTTGCCGAACCGGCATCAGCATGGAATTCACGATGACGCGAGAACCACATGGTGATa                              <  2:1324441/150‑1 (MQ=255)

                                                                                                                     tGCAGAGAGCCATCATGCTGGTTGCTTCTTACGGTTCATAGCTGGTTTTCAGGCGCTGCAGCGCGGCAATCATTTTCTCGCGACCAACCAGTTTTGCCGAACCGGCATCAGCATGGAATTCACGATGACGCGAGAACCACATGGTGATaa                             <  1:3725362/150‑1 (MQ=255)

                                                                                                                      gCAGAGAGCCATCATGCTGGTTGCTTCTTACGGTTCATAGCTGGTTTTCAGGCGCTGCAGCGCGGCAATCATTTTCTCGCGACCAACCAGTTTTGCCGAACCGGCATCAGCATGGAATTCACGATGACGCGAGAACCACATGGTGATAAt                            <  1:3002920/150‑1 (MQ=255)

                                                                                                                       cAGAGAGCCATCATGCTGGTTGCTTCTTACGGTTCATAGCTGGTTTTCAGGCGCTGCAGCGCGGCAATCATTTTCTCGCGACCAACCAGTTTTGCCGAACCGGCATCAGCATGGAATTCACGATGACGCGAGAACCACATGGTGATAATg                           >  1:1822702/1‑150 (MQ=255)

                                                                                                                       cAGAGAGCCATCATGCTGGTTGCTTCTTACGGTTCATAGCTGGTTTTCAGGCGCTGCAGCGCGGCAATCATTTTCTCGCGACCAACCAGTTTTGCCGAACCGGCATCAGCATGGAATTCACGATGACGCGAGAACCACATGGTGATAATg                           >  1:3046167/1‑150 (MQ=255)

                                                                                                                        agagagCCATCATGCTGGTTGCTTCTTACGGTTCATAGCTGGTTTTCAGGCGCTGCAGCGCGGCAATCATTTTCTCGCGACCAACCAGTTTTGCCGAACCGGCATCAGCATGGAATTCACGATGACGCGAGAACCACATGGTGATAATGc                          <  2:2241388/150‑1 (MQ=255)

                                                                                                                         gagagCCATCATGCTGGTTGCTTCTTACGGTTCATAGCTGGTTTTCAGGCGCTGCAGCGCGGCAATCATTTTCTCGCGACCAACCAGTTTTGCCGAACCGGCATCAGCATGGAATTCACGATGACGCGAGAACCACATGGTGATAATGCt                         <  1:426405/150‑1 (MQ=255)

                                                                                                                          agagCCATCATGCTGGTTGCTTCTTACGGTTCATAGCTGGTTTTCAGGCGCTGCAGCGCGGCAATCATTTTCTCGCGACCAACCAGTTTTGCCGAACCGGCATCAGCATGGAATTCACGATGACGCGAGAACCACATGGTGATAATGCTc                        <  1:1596877/150‑1 (MQ=255)

                                                                                                                          agagCCATCATGCTGGTTGCTTCTTACGGTTCATAGCTGGTTTTCAGGCGCTGCAGCGCGGCAATCATTTTCTCGCGACCAACCAGTTTTGCCGAACCGGCATCAGCATGGAATTCACGATGACGCGAGAACCACATGGTGATAATGCTc                        <  2:57816/150‑1 (MQ=255)

                                                                                                                          agagCCATCATGCTGGTTGCTTCTTACGGTTCATAGCTGGTTTTCAGGCGCTGCAGCGCGGCAATCATTTTCTCGCGACCAACCAGTTTTGCCGAACCGGCATCAGCATGGAATTCACGATGACGCGAGAACCACATGGTGATAATGCTc                        >  1:114975/1‑150 (MQ=255)

                                                                                                                            agCCATCATGCTGGTTGCTTCTTACGGTTCATAGCTGGTTTTCAGGCGCTGCAGCGCGGCAATCATTTTCTCGCGACCAACCAGTTTTGCCGAACCGGCATCAGCATGGAATTCACGATGACGCGAGAACCACATGGTGATAATGCTCGc                      <  1:132157/150‑1 (MQ=255)

                                                                                                                             gCCATCATGCTGGTTGCTTCTTACGGTTCATAGCTGGTTTTCAGGCGCTGCAGCGCGGCAATCATTTTCTCGCGACCAACCAGTTTTGCCGAACCGGCATCAGCATGGAATTCACGATGACGCGAGAACCACATGGTGATAATGCTCGcc                     >  1:2111585/1‑150 (MQ=255)

                                                                                                                              ccATCATGCTGGTTGCTTCTTACGGTTCATAGCTGGTTTTCAGGCGCTGCAGCGCGGCAATCATTTTCTCGCGACCAACCAGTTTTGCCGAACCGGCATCAGCATGGAATTCACGATGACGCGAGAACCACATGGTGATAATGCTCGCCa                    <  1:891164/150‑1 (MQ=255)

                                                                                                                               catcatGCTGGTTGCTTCTTACGGTTCATAGCTGGTTTTCAGGCGCTGCAGCGCGGCAATCATTTTCTCGCGACCAACCAGTTTTGCCGAACCGGCATCAGCATGGAATTCACGATGACGCGAGAACCACATGGTGATAATGCTCGCCAg                   >  1:450339/1‑150 (MQ=255)

                                                                                                                                atcatGCTGGTTGCTTCTTACGGTTCATAGCTGGTTTTCAGGCGCTGCAGCGCGGCAATCATTTTCTCGCGACCAACCAGTTTTGCCGAACCGGCATCAGCATGGAATTCACGATGACGCGAGAACCACATGGTGATAATGCTCGCCAGa                  >  2:3784685/1‑150 (MQ=255)

                                                                                                                                  catGCTGGTTGCTTCTTACGGTTCATAGCTGGTTTTCAGGCGCTGCAGCGCGGCAATCATTTTCTCGCGACCAACCAGTTTTGCCGAACCGGCATCAGCATGGAATTCACGATGACGCGAGAACCACATGGTGATAATGCTCGCCAGAAt                <  1:3811573/150‑1 (MQ=255)

                                                                                                                                  catGCTGGTTGCTTCTTACGGTTCATAGCTGGTTTTCAGGCGCTGCAGCGCGGCAATCATTTTCTCGCGACCAACCAGTTTTGCCGAACCGGCATCAGCATGGAATTCACGATGACGCGAGAACCACATGGTGATAATGCTCGCCAGAAt                <  1:2875278/150‑1 (MQ=255)

                                                                                                                                  catGCTGGTTGCTTCTTACGGTTCATAGCTGGTTTTCAGGCGCTGCAGCGCGGCAATCATTTTCTCGCGACCAACCAGTTTTGCCGAACCGGCATCAGCATGGAATTCACGATGACGCGAGAACCACATGGTGATAATGCTCGCCAGAAt                >  1:1932539/1‑150 (MQ=255)

                                                                                                                                  catGCTGGTTGCTTCTTACGGTTCATAGCTGGTTTTCAGGCGCTGCAGCGCGGCAATCATTTTCTCGCGACCAACCAGTTTTGCCGAACCGGCATCAGCATGGAATTCACGATGACGCGAGAACCACATGGTGATAATGCTCGCCAGAAt                <  2:2645678/150‑1 (MQ=255)

                                                                                                                                   atGCTGGTTGCTTCTTACGGTTCATAGCTGGTTTTCAGGCGCTGCAGCGCGGCAATCATTTTCTCGCGACCAACCAGTTTTGCCGAACCGGCATCAGCATGGAATTCACGATGACGCGAGAACCACATGGTGATAATGCTCGCCAGAATa               <  2:2058465/150‑1 (MQ=255)

                                                                                                                                      cTGGTTGCTTCTTACGGTTCATAGCTGGTTTTCAGGCGCTGCAGCGCGGCAATCATTTTCTCGCGACCAACCAGTTTTGCCGAACCGGCATCAGCATGGAATTCACGATGACGCGAGAACCACATGGTGATAATGCTCGCCAGAATACCa            <  1:2048860/150‑1 (MQ=255)

                                                                                                                                         gTTGCTTCTTACGGTTCATAGCTGGTTTTCAGGCGCTGCAGCGCGGCAATCATTTTCTCGCGACCAACCAGTTTTGCCGAACCGGCATCAGCATGGAATTCACGATGACGCGAGAACCACATGGTGATAATGCTCGCCAGAATACCAAac         >  1:602272/1‑150 (MQ=255)

                                                                                                                                         gTTGCTTCTTACGGTTCATAGCTGGTTTTCAGGCGCTGCAGCGCGGCAATCATTTTCTCGCGACCAACCAGTTTTGCCGAACCGGCATCAGCATGGAATTCACGATGACGCGAGAACCACATGGTGATAATGCTCGCCAGAATACCAAac         >  1:3370232/1‑150 (MQ=255)

                                                                                                                                          ttGCTTCTTACGGTTCATAGCTGGTTTTCAGGCGCTGCAGCGCGGCAATCATTTTCTCGCGACCAACCAGTTTTGCCGAACCGGCATCAGCATGGAATTCACGATGACGCGAGAACCACATGGTGATAATGCTCGCCAGAATACCAAaca        >  1:1399776/1‑150 (MQ=255)

                                                                                                                                          ttGCTTCTTACGGTTCATAGCTGGTTTTCAGGCGCTGCAGCGCGGCAATCATTTTCTCGCGACCAACCAGTTTTGCCGAACCGGCATCAGCATGGAATTCACGATGACGCGAGAACCACATGGTGATAATGCTCGCCAGAATACCAAaca        >  2:3125886/1‑150 (MQ=255)

                                                                                                                                              ttcttACGGTTCATAGCTGGTTTTCAGGCGCTGCAGCGCGGCAATCATTTTCTCGCGACCAACCAGTTTTGCCGAACCGGCATCAGCATGGAATTCACGATGACGCGAGAACCACATGGTGATAATGCTCGCCAGAATACCAAACACCAg    <  2:4059533/150‑1 (MQ=255)

                                                                                                                                               tcttACGGTTCATAGCTGGTTTTCAGGCGCTGCAGCGCGGCAATCATTTTCTCGCGACCAACCAGTTTTGCCGAACCGGCATCAGCATGGAATTCACGATGACGCGAGAACCACATGGTGATAATGCTCGCCAGAATACCAAACACCAGt   >  1:2225564/1‑150 (MQ=255)

TTTTAGTATTTACTTCATATTACTTCAGGTATTCACCCGTACGCAGAGCTTCAATTCGTTTATCCAGCGGCGGGTGGGTCATGAACAACTCACTGAGCGATTTCGACTTACCGTTAATGCAGAGAGCCATCATGCTGGTTGCTTCTTGCGGTTCATAGCTGGTTTTCAGGCGCTGCAGCGCGGCAATCATTTTCTCGCGACCAACCAGTTTTGCCGAACCGGCATCAGCATGGAATTCACGATGACGCGAGAACCACATGGTGATAATGCTCGCCAGAATACCAAACACCAGTT  >  AP012306/1591822‑1592115

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`