Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123062,714,5550TG24.3% 745.6 / 17.8 304T175P (ACC→CCC) ygjVconserved inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (100/130);  new base G (74/0);  total (174/130)
Fisher's exact test for biased strand distribution p-value = 2.87e-22
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 337 total aligned reads displayed.

CGTATTGCAGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAGCAGAAAACTACCCTCAATCATCGTGCCGCCTATCGACCCCGCCCAGAAGTTGTGAATCACCCAGCAACACGTTG  >  AP012306/2714407‑2714704
                                                                                                                                                    |                                                                                                                                                     
 gTATTGCAGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGttt                                                                                                                                                     >  2:3367729/1‑150 (MQ=255)
 gTATTGCAGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGgtt                                                                                                                                                     >  1:3099315/1‑150 (MQ=255)
  tATTGCAGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGtttt                                                                                                                                                    >  2:2333394/1‑150 (MQ=255)
  tATTGCAGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGtttt                                                                                                                                                    >  1:3776568/1‑150 (MQ=255)
   aTTGCAGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGgtttt                                                                                                                                                   >  2:3008238/1‑149 (MQ=255)
   aTTGCAGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTtc                                                                                                                                                   >  1:426285/1‑150 (MQ=255)
   aTTGCAGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGGTTtc                                                                                                                                                   >  2:3922006/1‑150 (MQ=255)
    ttGCAGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTtct                                                                                                                                                  >  1:1378614/1‑150 (MQ=255)
     tGCAGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTtctc                                                                                                                                                 >  1:110433/1‑150 (MQ=255)
     tGCAGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTtctc                                                                                                                                                 <  1:1778267/150‑1 (MQ=255)
      gCAGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCa                                                                                                                                                <  2:1054443/150‑1 (MQ=255)
       cAGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCaa                                                                                                                                               <  1:3462491/150‑1 (MQ=255)
           aCTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACttt                                                                                                                                           >  2:1324159/1‑150 (MQ=255)
             tctTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTaa                                                                                                                                         <  2:3566787/150‑1 (MQ=255)
             tctTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGGGTTCTCAACTTTaa                                                                                                                                         >  2:260713/1‑150 (MQ=255)
                tGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACg                                                                                                                                      <  2:3316301/150‑1 (MQ=255)
                 gCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACgg                                                                                                                                     <  1:3865772/150‑1 (MQ=255)
                 gCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACgg                                                                                                                                     >  1:2327721/1‑150 (MQ=255)
                     aaCCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGATCa                                                                                                                                 >  1:2572228/1‑150 (MQ=255)
                      aCCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGGTTTCTCCACTTTTAACGGGTCaa                                                                                                                                >  2:170344/1‑150 (MQ=255)
                        cgcgCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCGACTTTAAACGGATCAAtt                                                                                                                              >  1:116462/1‑150 (MQ=255)
                          cgcTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTcc                                                                                                                            <  1:3995682/150‑1 (MQ=255)
                          cgcTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGGTTTCTCAACTTTAAACGGATCAATTcc                                                                                                                            >  1:1034215/1‑150 (MQ=255)
                            cTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGGTTTCTCAACTTTAAACGGATCAATTcccc                                                                                                                          >  2:1067672/1‑150 (MQ=255)
                              gAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGGTTTTTCAACTTTAAAAGGATCAATTCCCCtt                                                                                                                        >  2:1585957/1‑150 (MQ=255)
                               aaCTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGCCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCttt                                                                                                                       <  2:2185192/150‑1 (MQ=255)
                                aCTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAACAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCtttt                                                                                                                      <  1:2168083/150‑1 (MQ=255)
                                  tctTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCt                                                                                                                    <  1:2891099/150‑1 (MQ=255)
                                    ttCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGc                                                                                                                  >  2:589584/1‑150 (MQ=255)
                                     tCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGGTTTCTCAACTTTTAACGGATCAATTCCCCCTTTCTGCa                                                                                                                 >  2:3696973/1‑150 (MQ=255)
                                      cAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCAt                                                                                                                >  2:1824125/1‑150 (MQ=255)
                                      cAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCAt                                                                                                                >  2:3557796/1‑150 (MQ=255)
                                         cTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGGTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCg                                                                                                             >  1:4098080/1‑150 (MQ=255)
                                           gACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGCAGGGGGGTTCTCAACTTTAAAAGGATTAATTCCCCTTTTCTTcgcccgcc                                                                                                           >  2:2743348/1‑150 (MQ=255)
                                              gTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGa                                                                                                        <  2:470907/150‑1 (MQ=255)
                                                     tCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAAt                                                                                                 >  1:895898/1‑150 (MQ=255)
                                                       aCTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGGTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGa                                                                                               >  2:2487260/1‑150 (MQ=255)
                                                           cGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATAtt                                                                                           >  1:1344368/1‑150 (MQ=255)
                                                              cAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAg                                                                                        <  1:390094/150‑1 (MQ=255)
                                                              cAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAg                                                                                        >  1:2821087/1‑150 (MQ=255)
                                                               aGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTTTCAACTTTAAACGGATCAATTCCCCTTTTTTGCATCCGCCAGAAACGAATGATAtttaag                                                                                       >  2:1242116/1‑146 (MQ=255)
                                                               aGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAgg                                                                                       <  1:1567973/150‑1 (MQ=255)
                                                                   tGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGGTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCCGGAACGAATGATATTCAGGCCac                                                                                   >  1:2108690/1‑149 (MQ=255)
                                                                    gCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCAtt                                                                                  >  1:3563875/1‑150 (MQ=255)
                                                                    gCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCAtt                                                                                  <  2:350918/150‑1 (MQ=255)
                                                                     ccAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTc                                                                                 <  1:712998/150‑1 (MQ=255)
                                                                      cAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGGTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCa                                                                                >  2:823991/1‑150 (MQ=255)
                                                                      cAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGGTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCa                                                                                >  2:2429706/1‑150 (MQ=255)
                                                                      cAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGGTTTCTCAACTTTAAACGGATCAAATTTCCTTTTCTGCATCCGCCCGAAACGAATGATATTCAAGCCATTCa                                                                                >  2:4117527/1‑150 (MQ=255)
                                                                         gCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATaa                                                                             <  1:607697/150‑1 (MQ=255)
                                                                          cAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATg                                                                                                <  2:2807658/130‑1 (MQ=255)
                                                                          cAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAg                                                                            >  1:1687276/1‑150 (MQ=255)
                                                                           agaagaCGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAGc                                                                           <  2:4148582/150‑1 (MQ=255)
                                                                           agaagaCGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAGc                                                                           <  2:1409475/150‑1 (MQ=255)
                                                                           agaagaCGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGGTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAGc                                                                           >  1:1587212/1‑150 (MQ=255)
                                                                                aCGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAGCAGaaa                                                                      >  1:3196596/1‑150 (MQ=255)
                                                                                 cGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAGCAGaaaa                                                                     <  1:2657190/150‑1 (MQ=255)
                                                                                 cGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCCTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCTTAAGCAGaaaa                                                                     >  2:2915415/1‑150 (MQ=255)
                                                                                  gATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGGTTTTTTAAATTTTAACGGAACAATTTCCCTTTTCTTCATCCGCCCGAAACGAATGATATTTAGGGCATTTTTCAGGAGaaaaa                                                                    >  2:3108676/1‑149 (MQ=255)
                                                                                  gATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGGTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCCTTCATAAGCAGAAAAc                                                                    >  1:521902/1‑150 (MQ=255)
                                                                                   aTCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAGCAGAAAACt                                                                   <  1:148508/150‑1 (MQ=255)
                                                                                   aTCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGGTTTCTCAACTTTAAACGGATCAATTCCCCTTTTTTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAGCAGAAAACt                                                                   >  2:4051667/1‑150 (MQ=255)
                                                                                     cGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAGCAGAAAACTAc                                                                 >  1:1168285/1‑150 (MQ=255)
                                                                                        cTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAGCAGAAAACTACCCt                                                              <  1:2976523/150‑1 (MQ=255)
                                                                                          ggCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAGCAGAAAACTACCCTCa                                                            >  1:470959/1‑150 (MQ=255)
                                                                                          ggCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAGCAGAAAACTACCCTCa                                                            >  1:2424333/1‑150 (MQ=255)
                                                                                          ggCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGGTTTTTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAGCAGAAAAATACCCTCa                                                            >  2:3399486/1‑150 (MQ=255)
                                                                                           gCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGGTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAGCAGAAAACTACCCTCaa                                                           >  2:1481330/1‑150 (MQ=255)
                                                                                             aaaTAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAGCAGAAAACTACCCTCAatc                                                         >  1:3491213/1‑150 (MQ=255)
                                                                                             aaaTAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGGTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAACAGAAAAATACCCTCAatc                                                         >  2:543199/1‑150 (MQ=255)
                                                                                              aaTAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAGCAGAAAACTACCCTCAatca                                                        <  1:1922940/150‑1 (MQ=255)
                                                                                                 aGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGGTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAGCAGAAAACTACCCTCAATCATCg                                                     >  1:1058947/1‑150 (MQ=255)
                                                                                                    gcCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGGTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAGCAGAAAACTACCCTCAATCATCGTgc                                                  >  2:213400/1‑150 (MQ=255)
                                                                                                     cccTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGGTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAGCAGAAAACTACCCTCAATCATCGTgcc                                                 >  2:3262653/1‑150 (MQ=255)
                                                                                                       cTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGGTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAGCAGAAAACTACCCTCAATCATCGTgccgc                                               >  1:2344744/1‑150 (MQ=255)
                                                                                                          cGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAGCAGAAAACTACCCTCAATCATCGTGCCGCCTa                                            <  1:2549793/150‑1 (MQ=255)
                                                                                                          cGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAGCAGAAAACTACCCTCAATCATCGTGCCGCCTa                                            <  1:3793399/150‑1 (MQ=255)
                                                                                                            taattaatTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGGTTTCTCAACTTTAAAAGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAGCAGAAAACTACCCTCAATCATCGTGCCGCCTATc                                          >  2:3763163/1‑150 (MQ=255)
                                                                                                                taattGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAGCAGAAAACTACCCTCAATCATCGTGCCGCCTATCGAcc                                      >  2:4013884/1‑150 (MQ=255)
                                                                                                                taattGTTTAACCCCTTTCGTCTACGGCGGAAGGGGGTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAGCAGAAAACTACCCTCAATCATCGTGCCGCCTATCGAcc                                      >  2:3624981/1‑150 (MQ=255)
                                                                                                                 aattGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAGCAGAAAACTACCCTCAATCATCGTGCCGCCTATCGAccc                                     <  1:209198/150‑1 (MQ=255)
                                                                                                                  attGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTTTCCACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAGCAGAAAACTACCCTCAATCATCGTGCCGCCTATCGAcccc                                    >  1:2725709/1‑150 (MQ=255)
                                                                                                                  attGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAGCAGAAAACTACCCTCAATCATCGTGCCGCCTATCGAcccc                                    <  1:627130/150‑1 (MQ=255)
                                                                                                                   ttGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAGCAGAAAACTACCCTCAATCATCGTGCCGCCTATCGACCCCg                                   <  1:3535443/150‑1 (MQ=255)
                                                                                                                    tGTTTAACCCCTTTCGTCTACGGCGGAAAGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCC‑CCAGAAACGGATGATATTCAGGCCATTCATAAGCAGAAAACTACCCTCCATCATCGTGCCGCCTATCGACCCCGcc                                 >  1:1664941/1‑150 (MQ=255)
                                                                                                                        tAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAGCAGAAAACTACCCTCAATCATCGTGCCGCCTATCGACCCCGCCCAg                              <  1:1007384/150‑1 (MQ=255)
                                                                                                                          aCCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGGTCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAGCAGAAAACTACCCTCAATCATCGTGCCGCCTATCGAACCCGCCCAGaa                            >  1:1971058/1‑150 (MQ=255)
                                                                                                                           ccccTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAGCAGAAAACTACCCTCAATCATCGTGCCGCCTATCGACCCCGCCCAGAAg                           <  2:2026342/150‑1 (MQ=255)
                                                                                                                            cccTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAGCAGAAAACTACCCTCAATCATCGTGCCGCCTATCGACCCCGCCCAGAAGt                          <  1:1225010/150‑1 (MQ=255)
                                                                                                                            cccTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAGCAGAAAACTACCCTCAATCATCGTGCCGCCTATCGACCCCGCCCAGAAGt                          >  1:3346261/1‑150 (MQ=255)
                                                                                                                            cccTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAGCAGAAAACTACCCTCAATCATCGTGCCGCCTATCGACCCCGCCCAGAAGt                          <  1:970844/150‑1 (MQ=255)
                                                                                                                                ttCGTCTACGGCGGAAGGGGGTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAGCAGAAAACTACCCTCAATCATCGTGCCGCCTATCGACCCCGCCCAGAAGTtgtg                      >  2:3183173/1‑150 (MQ=255)
                                                                                                                                     cTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAGCAGAAAACTACCCTCAATCATCGTGCCGCCTATCGACCCCGCCCAGAAGTTGTGAATCa                 >  2:1748690/1‑150 (MQ=255)
                                                                                                                                      tACGGGGGAAGGGGTTTTCTCAACTTTAAACGGGTCAATTCCCCTTTTCTGCATCCCCCAGAAACGAATGATATTCAGGCCATTCATAAGCAGAAAACTACCCTCAATCATCGTGCCGCCTATCGACCCCGCCCAGAAGTTGTGAATCAc                >  1:2232046/1‑150 (MQ=255)
                                                                                                                                      tACGGCGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAGCAGAAAACTTCCCTCAATCATCGTGCCGCCTATCGACCCCGCCCAGAAGTTGTGAATCAc                <  1:2806877/150‑1 (MQ=255)
                                                                                                                                        cggcggAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAGCAGAAAACTACCCTCAATCATCGTGCCGCCTATCGACCCCGCCCAGAAGTTGTGAATCAccc              >  2:748758/1‑150 (MQ=255)
                                                                                                                                               aGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAGCAGAAAACTACCCTCAATCATCGTGCCGCCTATCGACCCCGCCCAGAAGTTGTGAATCACCCAGCAaca       >  2:1665113/1‑150 (MQ=255)
                                                                                                                                                  ggTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAGCAGAAAACTACCCTCAATCATCGTGCCGCCTATCGCCCCCGCCCAGAAGTTGTGAATCACCCAGCAACACGt    <  1:323466/150‑1 (MQ=255)
                                                                                                                                                  ggTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAGCAGAAAACTACCCTCAATCATCGTGCCGCCTATCGACCCCGCCCAGAAGTTGTGAATCACCCAGCAACACGt    <  1:3675897/150‑1 (MQ=255)
                                                                                                                                                   gTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAGCAGAAAACTACCCTCAATCATCGTGCCGCCTATCGACCCCGCCCAGAAGTTGTGAATCACCCAGCAACACGtt   <  2:1988948/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
CGTATTGCAGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAGCAGAAAACTACCCTCAATCATCGTGCCGCCTATCGACCCCGCCCAGAAGTTGTGAATCACCCAGCAACACGTTG  >  AP012306/2714407‑2714704

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑