Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123063,182,8610AC25.4% 284.6 / 23.5 114L198F (TTA→TTC) yiaNpredicted transporter
Reads supporting (aligned to +/- strand):  ref base A (72/13);  new base C (0/29);  total (72/42)
Fisher's exact test for biased strand distribution p-value = 2.50e-17
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 176 total aligned reads displayed.

TTATTCCCCCTTCCATTCCGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGC‑GGGCATTGCCCCTGGCATGATG‑ATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCG‑CC‑TTAA‑TTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTG‑TTTACCCCTACCGAAGCCGGGGCCGTTGCTGCTTTCTATG  >  AP012306/3182713‑3183009
                                                                                                                                                         |                                                                                                                                                     
 tattCCCCCTTCCATTCCGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGC‑GGGCATTGCCCCTGGCATGATG‑ATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCG‑CC‑TTAA‑TTTAcc                                                                                                                                                     <  1:4025820/150‑1 (MQ=255)
  attCCCCCTTCCATTCCGTTTATTATTTTCCGCGTTTCCAGCGGATTATTCATCAGTAAGCTGTTTATGGC‑GGGCATTGCCCCTGGCATGATG‑ATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCG‑CC‑TTAA‑TTTAccc                                                                                                                                                    <  1:2338117/150‑1 (MQ=255)
        ccTTCCATTCCGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGC‑GGGGGATTCCCCTGGCCTTGTG‑ATTGGCGCCACCCTGATGCTTACCTGGTGGGGGGAGGGCCGACG‑CC‑CTAATTTTACccccccg                                                                                                                                               >  2:3009468/1‑149 (MQ=255)
         cTTCCATTCCGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGC‑GGGCATTGCCCCCGGCATGATG‑ATGGGCGCAACGCTGATGCTTTCCTGGTGGGGGGGGGGCAGTCGCCC‑TTAT‑TTTTCcccccccc                                                                                                                                              >  2:1164239/1‑148 (MQ=255)
          ttCCATTCCGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGC‑GGGCATTTCCCCCGGCACGATT‑TTGGGGGCAAAGCCGATGCCTTCCCGGTGGTGGGGGGCCAGGCG‑CC‑CTTA‑TTTTCCCCCCCCGcc                                                                                                                                            >  2:1681851/1‑149 (MQ=255)
           tCCATTCCGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGC‑GGGCGTTTTCCCTGGCATGATG‑TGGGGTGCGACGCTTTTGCTTATCTTGGGGGGGCGGGCCCGTCG‑CCGTTAA‑TTT‑CCCCGcccacaa                                                                                                                                           >  2:3949817/1‑145 (MQ=255)
             cATTCCGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGC‑GGGCATTTCCCCCGGCATTATG‑ATTGGGGCCACGCCGGTGGTTTCCCTGGGGGGGGAGGGCCGGCG‑CC‑CTAA‑TTTTTCCCGccccccaaa                                                                                                                                         >  2:1801600/1‑143 (MQ=255)
              aTTCCGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGC‑GGGCAATGGACCCGGCATGTTGTGTTGGC‑CAACACAGAGGCTTACCTGGCGGCGGCTGGCGCAACG‑CC‑TCAA‑TTTACCCCTCCAGCgaaaa                                                                                                                                        >  2:138407/1‑150 (MQ=255)
                 cgttttATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGC‑GGGCATTGCCCCTGGCATGATG‑ATGGGCGCAACGCTGATGCTTCCCTGGTGGGGGCAGGCCAGTCG‑CC‑TTAA‑TTTCCCCCGCCAGCAAAAAGCa                                                                                                                                     <  1:2138447/147‑1 (MQ=255)
                      tattatCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGC‑GGGCGTTGCCCCCGGCATGATG‑ATGGGGGCAACGCTGATTCTTTCCTGGGGGTGGGGGGCCCGTCG‑CC‑TTTA‑TTTTTTCCCCCCGCAAAAAACAACGAt                                                                                                                                >  2:2814175/1‑150 (MQ=255)
                          atCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGC‑GGGCGTTTCCCCCCGCCTGTTG‑ATGGGGGCAACGCTGATGGTTTCCTTGGGGGGGGGGGGCCGGCG‑CC‑TTTA‑TTTTTCCCCCCCGCAAAAAAAAACGATGcgg                                                                                                                            >  2:3640442/1‑148 (MQ=255)
                          atCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGC‑GGGCCTTTCCCCCGGCCTGATT‑ATTGGGGCAAACCTGATGCTTACCTGGTGGTGGGAGGCCAGTCG‑CC‑CTTA‑TTTATCCCCCCCGCAAAAAACAAAGATGCAg                                                                                                                            >  2:2377620/1‑150 (MQ=255)
                          atCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGC‑GGGCATTTCCCCCCGCATGTTT‑ATTGGGGCCACCCTGATGCTTACCCGGTGGGGGGAGGCCAGCCG‑CC‑TTAA‑TTTAACCCCCCCGCAAAAAACAAAGATTCat                                                                                                                            >  2:12708/1‑149 (MQ=255)
                            cTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGC‑GGGCATTGCCCCTGGCATGATG‑ATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCG‑CC‑TTAA‑TTTACCCCGCCAGCAAAAAGCAACGATGCAGGa                                                                                                                          <  1:1347056/150‑1 (MQ=255)
                              tCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGC‑GGGCGTTTCCCCTGGCGTTAAT‑ATTGGCGCAACGCTGATGCTTTTCTTGTGGGGGGAGGGCAGTCG‑CC‑TTTA‑TTTAACCCCCCCGCAAAAAACAACGAAGCAGGgaa                                                                                                                        >  2:3212608/1‑150 (MQ=255)
                                ggCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGC‑GGGCGTTTCCCCCGGCATGATG‑ATGGGGGCAACGCTGATGCTTTCCTGGTGGGGGCAGGGCAGGCG‑CC‑CTAA‑TTTTCCCCCCCCGCAAAAAAAAACGAAGCAGGaaaac                                                                                                                      >  2:3257294/1‑148 (MQ=255)
                                 gCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGC‑GGGCATTGCCCCGGGCATGATG‑ATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCG‑CC‑TTAA‑TTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCt                                                                                                                     <  1:3527609/150‑1 (MQ=255)
                                   gTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGC‑GGGCATTGCCCCCGGCATGGTG‑ATGGGGGCAACGCTGATGCTTACCTGGGGGGGGCAGGCCAGTCG‑CC‑TTTA‑TTTACCCCGCCCCCCAAAAACAACGATGCCGGAAAACTgg                                                                                                                   >  2:86898/1‑150 (MQ=255)
                                          gCGGATTATCCATCAGTAAGCTGTTTATGGC‑GGGCATTGCCCCTGGCATGATG‑ATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCG‑CT‑TTAA‑TTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCtt                                                                                                            <  1:1359876/150‑1 (MQ=255)
                                           cGGATTATCCATCAGTAAGCTGTTTATGGC‑GGGCATTGCCCCGGGCATGATG‑ATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCG‑CT‑TTAA‑TTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCttt                                                                                                           <  2:721999/150‑1 (MQ=255)
                                            ggATTATCCATCAGTAAGCTGTTTATGGC‑GGGGCCTGCCCCTGGCCTGGTT‑GAGGGGGCGACACTGCTGCTTTACTCGTGGCGGCGCGGCCGCGC‑CC‑TTAA‑TTTATCCCGCCCGCCAAAAACAACGACGCAAGGAAACTGGGACTCtttt                                                                                                          >  2:1505389/1‑150 (MQ=255)
                                            ggATTATCCATCAGTAAGCTGTTTATGGC‑GGGCATTGCCCCCGGCCTGATG‑ATGGGGGCAACGCTGATGCTTACCTGGTGGGGGCAGGGCAGGCG‑CC‑TTAA‑TTTACCCCCCCCGCAAAAAACAACGATGCAGGAAAACTGGCACTCtttt                                                                                                          >  2:2613000/1‑150 (MQ=255)
                                              aTTATCCATCAGTAAGCTGTTTATGGCGGGGGATTG‑CCCTGGCTTGTTT‑TTTGGGGCAACGCTGAGGCTTTCCTGGTGGTGGCAGGCCCGTCG‑CC‑TTTA‑TTTACCCCCCCCGCGAAAACGGAAGAAGCAAGAAATCTCGCGCTCtttttt                                                                                                        >  2:269788/1‑148 (MQ=255)
                                              aTTATCCATCAGTAAGCTGTTTATGGC‑GGGGATTTCCCCCTGCATGATT‑ATGGGGGCAACGCTGATGCTTTCCTGGTGGGGGGCGGCCCGTCG‑CC‑CTAA‑TTTATCCCCCCCGCCAAAAACAACGATTCAGGGAATCCTGGACTCtttttt                                                                                                        >  2:2088125/1‑148 (MQ=255)
                                               ttATCCATCAGTAAGCTGTTTATGGC‑GGGCCTTGCCCCCGGCCTGATG‑ATGGGGGCCACGCTGATGCTTTCCTGGTGGGGGGGGGCCAGGCG‑CC‑TTAA‑TTTACCCCCCCCCCCAAAAAAAAAGAGGGCGGAAAACTGGGCCTCttttttt                                                                                                       >  2:1040785/1‑147 (MQ=255)
                                                tATCCATCAGTAAGCTTTTTATGGC‑GGGCTTTGCCCCTGGCATGATG‑AGGGGCGCACCGCTGATCTTTCCCGGGTGGGGGCAGCCCAGTCG‑CT‑TTAT‑TTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGtct                                                                                                      <  2:2873370/150‑1 (MQ=255)
                                                tATCCATCAGTAAGCTGTTTATGGC‑GGGCCTTTCCCCTCGCCTTTTG‑ATTGGGGCAACACTTATGTTTACCTGGTGGGGGGGGGCCAGTCG‑CC‑TTTA‑TTTTCCCCCCCCGCAAAAAAAAAAGAAGCAGGAAAACTGGCACCCtttttttt                                                                                                      >  2:2573554/1‑146 (MQ=255)
                                                tATCCATCAGTAAGCTGTTTATGGC‑GGGCATTTCCCCCGGCATGATG‑ATGGGGGCAACGCTGATGCTTTACTGGTGGGGGGGGGGCAGGCG‑CC‑TTAA‑TTTATTCCCCCCGCCAAAAAAAAAGATGCAGGAAATCTGGGCATCtttttttt                                                                                                      >  2:668681/1‑146 (MQ=255)
                                                   cccccAGTAAGTTTTTTATGGC‑GGGCTTTGCCCCTGGCATGAGG‑AGGGGCGCAACGCTGATGCTTACCTGGGGGTGGCAGGCCAGTCG‑CC‑TTAA‑TTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCg                                                                                                   <  2:521719/146‑1 (MQ=255)
                                                   ccATCAGTAAGCTGTTTATGGC‑GGGCATTGCCCCCGGGATGGTG‑GTGGGGGGAACGCTGATGCTTTCCTTGGGGGGGGGGGGCAGGGG‑CC‑TTTA‑TTTACCCCCCCCGCCAAAAACAAAGATGAAGGGAAACTGGGACTCTTTTTTTTccc                                                                                                   >  2:3069989/1‑149 (MQ=255)
                                                     aTCAGTAAGTTTTTTTTGGC‑GGGCATTGCCCCTGGCATGATG‑AGGGGCGCACCGCTGATGCTTACCTGGTGGGGGCAGGCCAGTCG‑CT‑TTAT‑TTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGt                                                                                                 <  2:1349180/150‑1 (MQ=255)
                                                          tAAGCTGTTTATGGC‑GGGCATTGCCCCTGGCATGATG‑ATGGGCGCAACGCTGATGCTTTCCTGGGGGGGGGAGGCCCGGCG‑CC‑CTAA‑TTTACCCCCCCCGCAAAAAAAAACGATGCAGGAAATCTGGCACTCTTTTTTTTCCGGGATCtt                                                                                            >  2:3183448/1‑149 (MQ=255)
                                                            aGCTGTTTATGGC‑GGGCATTGCCCCTGGCATGATG‑ATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCG‑CC‑TTAA‑TTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTggg                                                                                          <  1:1157386/150‑1 (MQ=255)
                                                              cTGTTTATGGC‑GGGCATTTCCCCCGGCATGATG‑TTGGGGGCAACGCTGATGCTTTCCTGGTGGGGGGAGGGCCGGCG‑CC‑CTAA‑TTTACCCCCCCCGCAAAAAAAAACGATGCAGGAAATCTGGCACTCTTTTTTCTCCGGGATCTGGgcg                                                                                        >  2:2783388/1‑150 (MQ=255)
                                                               tGTTTATGGC‑GGGCATTGCCCCCGGCCTGATG‑ATGGGGGCAACGCTGATGCTTTCCTGGTGGGGGGAGGGCAGGCG‑CC‑TTAA‑TTTTACCCCCCAGCAAAAAACAACGATGCAGGAAAACTGGGACTCTTTTTTTTCCCGTATCTGGgcgc                                                                                       >  2:3318170/1‑150 (MQ=255)
                                                                gTTTATGGT‑GGGCATTGCCCCTGGCATGATG‑ATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCG‑CC‑TTAA‑TTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCt                                                                                      >  2:2221665/1‑150 (MQ=255)
                                                                   ttgggC‑GGGCATTGCCCCTGGCATGATG‑ATGGGCGCAACGCTGATGCTTCCTTGGTGGGGGCAGGCCAGTCG‑CC‑TTAA‑TTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGtt                                                                                   <  2:3563595/147‑1 (MQ=255)
                                                                     tGGC‑GGGCATTGCCCCTGGCATGATG‑ATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCG‑CC‑TTAA‑TTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTttc                                                                                 >  2:2611456/1‑150 (MQ=255)
                                                                          gggCATTGCCCCTGGCATGATG‑ATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCG‑CC‑TTAA‑TTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTcc                                                                             >  1:1384066/1‑150 (MQ=255)
                                                                             cATTGCCCCTGGCATGATG‑ATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCG‑CC‑TTAA‑TTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGt                                                                          >  2:3438784/1‑150 (MQ=255)
                                                                             cATTGCCCCTGGCATGATG‑ATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCG‑CC‑TTAA‑TTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGt                                                                          >  1:3135642/1‑150 (MQ=255)
                                                                              aTTGCCCCTGGCATGATG‑ATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCG‑CC‑TTAA‑TTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGtc                                                                         >  2:2804243/1‑150 (MQ=255)
                                                                               ttGCCCCTGGCATGATG‑ATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCG‑CC‑TTAA‑TTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGtca                                                                        >  2:2393330/1‑150 (MQ=255)
                                                                                 gCCCCTGGCATGATG‑ATGGGCCCACCGCTGATGCTTCCCTGGTGGTGGCGGGCCAGTCG‑CT‑TTAA‑TTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGtcatc                                                                      <  2:3490761/150‑1 (MQ=255)
                                                                                    ccTGGCATGATG‑ATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCG‑CC‑TTAA‑TTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCatt                                                                   >  1:3485459/1‑150 (MQ=255)
                                                                                    ccTGGCATGATG‑ATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCG‑CC‑TTAA‑TTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCatt                                                                   >  2:1600480/1‑150 (MQ=255)
                                                                                      tGGCATGATG‑ATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCG‑CC‑TTAA‑TTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCattat                                                                 >  1:142903/1‑150 (MQ=255)
                                                                                      tGGCATGATG‑ATGCGCGCAACTCTGATGCTTCCCTGTTGGTGGCCGGCCAGTCG‑CT‑TTAT‑TTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCattat                                                                 <  2:2242643/150‑1 (MQ=255)
                                                                                       ggCATGATG‑ATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCG‑CC‑TTAA‑TTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCattatt                                                                >  1:1463208/1‑150 (MQ=255)
                                                                                        gCATGATG‑ATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCG‑CC‑TTAA‑TTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTg                                                               >  2:2281093/1‑150 (MQ=255)
                                                                                           tgatg‑atgGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCG‑CC‑TTAA‑TTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCg                                                            >  2:2539611/1‑150 (MQ=255)
                                                                                            gatg‑atgGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCG‑CC‑TTAA‑TTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCgg                                                           >  2:1237017/1‑150 (MQ=255)
                                                                                            gatg‑aggGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCG‑CC‑TTAA‑TTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCgg                                                           <  1:255344/150‑1 (MQ=255)
                                                                                             atg‑atgGGCGCAACGCTGATGCTTCCCTGGTGGTGGCAGGCCAGTCG‑CC‑TTAA‑TTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCggg                                                          <  1:811169/150‑1 (MQ=255)
                                                                                             atg‑atgGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCG‑CC‑TTAA‑TTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCggg                                                          <  1:348026/150‑1 (MQ=255)
                                                                                              tg‑atgGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCG‑CC‑TTAA‑TTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCgggg                                                         >  1:1967748/1‑149 (MQ=255)
                                                                                                  ggggCGCACCGTTGGTGCTTACCTGGTGGTGGCGGCCCAGTCG‑CT‑TTAT‑TTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTc                                                      <  2:1904480/149‑1 (MQ=255)
                                                                                                  ggggCGCAACGCTGATGCTTACCGGGGGGGGGCAGGCCAGTCG‑CC‑TTAA‑TTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTc                                                      <  2:696613/149‑1 (MQ=255)
                                                                                                    ggCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCG‑CC‑TTAA‑TTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGtcttcc                                                    >  2:3912973/1‑145 (MQ=255)
                                                                                                      cgcAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCG‑CC‑TTAA‑TTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTTGccc                                                  >  1:2495295/1‑148 (MQ=255)
                                                                                                       gcAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCG‑CT‑TTAA‑TTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTcc                                                 <  1:1213037/150‑1 (MQ=255)
                                                                                                        cAACGTTGATGTTTACTGGGTGGTGGCAGGCCAGTGG‑CT‑TTAT‑TTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCg                                                <  2:2994019/150‑1 (MQ=255)
                                                                                                        cAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCG‑CC‑TTAA‑TTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTccc                                                >  1:3185847/1‑149 (MQ=255)
                                                                                                            gCTGATGCTTACCTGGTGGTGGCAGGCCAGTCG‑CC‑TTAA‑TTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCt                                            <  1:3179707/150‑1 (MQ=255)
                                                                                                            gCTGATGCTTACCTGGTGGTGGCAGGCCAGTCG‑CC‑TTAA‑TTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTTGCTCCCGCCt                                            >  2:2958366/1‑150 (MQ=255)
                                                                                                              tGATGCTTCCGTGGGGGTGGCAGGCCAGTCG‑CC‑TTAT‑TTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTG‑t                                         <  2:3948189/150‑1 (MQ=255)
                                                                                                              tGATGCTTACCTGGTGGTGGCAGGCCAGTCG‑CC‑TTAA‑TTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTTGCTCCCGCCCG‑t                                         >  2:3631259/1‑150 (MQ=255)
                                                                                                              tGATGCTTACCTGGTGGTGGCAGGCCAGTCG‑CC‑TTAA‑TTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTTCCTTCCGCCTG‑t                                         >  1:1018287/1‑150 (MQ=255)
                                                                                                              tGATGCTTACCTGGTGGTGGCAGGCCAGTCG‑CC‑TTAA‑TTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGGTTTGCTcccggccgt                                          >  2:2229219/1‑143 (MQ=255)
                                                                                                              tGATGCTTACCTGGTGGTGGCAGGCCAGTCG‑CC‑TTAA‑TTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGGTTTGCTCCCGcccgg                                          >  2:2178742/1‑147 (MQ=255)
                                                                                                                 tGCTTCCCTGGTGGTGGCAGGCCAGTCG‑CC‑TTAA‑TTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTG‑TTTa                                      <  1:2680617/150‑1 (MQ=255)
                                                                                                                       ccTGGTGGTGGCAGGCCAGTCG‑CC‑TTAA‑TTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGGTTCGCGCCGGCCTC‑tttttcccca                                >  1:170527/1‑143 (MQ=255)
                                                                                                                        gggggggtggCAGCCCAGTCG‑CC‑TTAT‑TTCCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTG‑TTTACCCCTAc                               <  2:2918221/145‑1 (MQ=255)
                                                                                                                          ggtggtggCAGGCCAGTCG‑CC‑TTAA‑TTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGGTTTGCTCCGGCCCG‑TTTATCCCTAccc                             >  1:3728536/1‑149 (MQ=255)
                                                                                                                             gggggCAGGCCGGTGG‑CC‑TTAA‑TTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTG‑TTTACCCCTACCGAAg                          <  1:1602357/147‑1 (MQ=255)
                                                                                                                                  cAGGCCAGTCG‑CC‑TTAA‑TTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTG‑TTTACCCCTACCGAAGCCggg                     <  2:3824921/150‑1 (MQ=255)
                                                                                                                                    ggCCAGTCG‑CC‑TTAA‑TTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTG‑TTTACCCCTACCGAAGCCGGGGc                   <  1:2733088/150‑1 (MQ=255)
                                                                                                                                    ggCCAGTCG‑CC‑TTAA‑TTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGGTTTGCTCCCGCCCG‑TTTTTCCCCCCCCCAGCCGGGGc                   >  2:1865641/1‑150 (MQ=255)
                                                                                                                                      ccAGTCG‑CC‑TTAA‑TTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGGTTTGCTCCCGCCCG‑TTTTTCCCTCCCCAAACCCGGGGCg                 >  2:944710/1‑150 (MQ=255)
                                                                                                                                      ccAGTCG‑CA‑TTAA‑TTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTTGCTCCGGCCTG‑GTTTCCCCTCCCGAAGCCCGGGCCg                 >  2:3429833/1‑150 (MQ=255)
                                                                                                                                         gccG‑CC‑TTAA‑TTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTG‑TTTACCCCTACCGAAGCCGGGGCCGTtg              <  1:3516177/148‑1 (MQ=255)
                                                                                                                                         gTCG‑CC‑TTAA‑TTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTG‑TTTACCCCTACCGAAGCCGGGGCCGTtg              <  1:2628362/150‑1 (MQ=255)
                                                                                                                                         gTCG‑CC‑TTAA‑TTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCTCTCCCGCCCG‑TTTTCCCCCACCCCAGCCCGGGCCGGtg              >  1:389644/1‑150 (MQ=255)
                                                                                                                                         gTCG‑CC‑TTAA‑TTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTG‑TTTACCCCTACCGAAGCCGGGGCCGTtg              <  1:519642/150‑1 (MQ=255)
                                                                                                                                           gtccctttAA‑TTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTG‑TTTACCCCTACCGAAGCCGGGGCCGTtg              <  2:3154707/144‑1 (MQ=255)
                                                                                                                                           cG‑CC‑TTAA‑TTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTTGCTCCCGCCCG‑TTTTCCCCCACCCCAGCCGGGGCCgctgct            >  1:576260/1‑150 (MQ=255)
                                                                                                                                           cG‑CC‑TTAA‑TTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTG‑TTTACCCCTACCGAAGCCGGGGCCGTtgct            <  1:2804243/150‑1 (MQ=255)
                                                                                                                                            g‑CC‑TTAA‑TTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTG‑TTTACCCCTACCGAAGCCGGGGCCGTtgctg           <  1:1229249/150‑1 (MQ=255)
                                                                                                                                            g‑CC‑TTAA‑TTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGGTTTGCTCCCGGCTGTTTTACCCCACCCCAACCCGGGGcgctggtg            >  1:3231672/1‑143 (MQ=255)
                                                                                                                                            g‑CC‑TTAA‑TTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGGTTTGCTCCCGCCTG‑TTTACCCCCACCCAAGCCGGGGCCGGtgctg           >  1:3367114/1‑150 (MQ=255)
                                                                                                                                              cc‑TTAA‑TTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCCGCCTG‑TTTACCCCCCCCCAAGCCGGGGCCGTtgctgc          >  1:2775161/1‑150 (MQ=255)
                                                                                                                                               c‑TTAA‑TTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGGTTTGGTCCCGGCCG‑TTTTTCCCTACCCGAGCCGGGGCCGGtgctgcc         >  1:13708/1‑149 (MQ=255)
                                                                                                                                                 ttAA‑TTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCGCCGGCCTG‑CTTTCCCCTCCCGAACCCCGGGGCGCTGCTGCtt        >  1:750441/1‑150 (MQ=255)
                                                                                                                                                  tAA‑TTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGGTTTTCTCCCGCCGG‑TTTTCCCCTCCCAAAACCCGGGGCGTTGCTGCttt       >  1:1851121/1‑150 (MQ=255)
                                                                                                                                                  tAA‑TTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGGTTTGCTCCCGCCCG‑TTTTCCCCCACCCCAGCCGGGGGCGCTGCTGCttt       >  1:963489/1‑150 (MQ=255)
                                                                                                                                                    attttCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTG‑TTTACCCCTACCGAAGCCGGGGCCGTTGCTGCTTTc      <  1:518473/148‑1 (MQ=255)
                                                                                                                                                      tttACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTTGCTTCCGCCCG‑TTTTTCCCCCCCCAAACCCGGGGCGGTGCTGCtttttt    >  2:1522763/1‑147 (MQ=255)
                                                                                                                                                       ttACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGGTTTGCTCCGGCCTG‑TTTTTCCCCACCCAAACCGGGGGCGGTGCTTCttttttt   >  2:505231/1‑146 (MQ=255)
                                                                                                                                                        tACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCCCCGGCCCG‑TTTTAACCCACCCCACCCGGGGGCGTTGCTGCgttttttg  >  1:2926442/1‑145 (MQ=255)
                                                                                                                                                        tACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGGTTTTCCCCCGCCCG‑TTTTCCCCCCCCCCAACCCGGGCCCCTGCTGCtttttttt  >  1:3727587/1‑145 (MQ=255)
                                                                                                                                                         |                                                                                                                                                     
TTATTCCCCCTTCCATTCCGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGC‑GGGCATTGCCCCTGGCATGATG‑ATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCG‑CC‑TTAA‑TTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTG‑TTTACCCCTACCGAAGCCGGGGCCGTTGCTGCTTTCTATG  >  AP012306/3182713‑3183009

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑