Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123063,590,3670AC25.4% 734.4 / 44.1 276T30P (ACC→CCC) fabRDNA‑binding transcriptional repressor
Reads supporting (aligned to +/- strand):  ref base A (189/17);  new base C (0/70);  total (189/87)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 315 total aligned reads displayed.

AGAATTGCAGTAAATATGTTTTATTGCGTTACCGTTCATTCACAATACTGGAGCAATCCAGTATGTTCATTCTCTGGTATAGTGCCAG‑CAGTACTTTTGGCAAGG‑ATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAA‑‑CCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGGCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGTCTG  >  AP012306/3590218‑3590516
                                                                                                                                                       |                                                                                                                                                       
 gAATTGCAGTAAATATGTTTTATTGCGTTACCGTTCATTCACAATACTGGAGCAATCCAGTATGTTCATTCTCTGGTATAGTGCCAG‑CAGTACTTTTGGCAAGG‑ATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAA‑‑c                                                                                                                                                      >  1:3653233/1‑150 (MQ=255)
 gAATTGCAGTAAATATGTTTTATTGCGTTACCGTTCATTCACAATACTGGAGCAATCCAGTATGTTCATTCTCTGGTATAGTGCCAG‑CAGTACTTTTGGCAAGG‑ATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAA‑‑c                                                                                                                                                      <  1:2393601/150‑1 (MQ=255)
  aaTTGCAGTAAATATGTTTTATTGCGTTACCGTTCATTCACAATACTGGAGCAATCCAGTATGTTCATTCTCTGGTATAGTGCCAG‑CAGTACTTTTGGCAAGG‑ATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAA‑‑cc                                                                                                                                                     >  1:587590/1‑150 (MQ=255)
  aaTTGCAGTAAATATGTTTTATTGCGTTACCGTTCATTCACAATACTGGAGCAATCCAGTATGTTCATTCTCTGGTATAGTGCCAG‑CAGTACTTTTGGCAAGG‑ATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAA‑‑cc                                                                                                                                                     >  2:64362/1‑150 (MQ=255)
   aTTGCAGTAAATATGTTTTATTGCGTTACCGTTCATTCACAATACTGGAGCAATCCAGTATGTTCATTCTCTGGTATAGTGCCAG‑CAGTACTTTTGGCAAGG‑ATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAA‑‑ccc                                                                                                                                                    <  1:3969529/150‑1 (MQ=255)
     tGCAGTAAATATGTTTTATTGCGTTACCGTTCATTCACAATACTGGAGCAATCCAGTATGTTCATTCTCTGGTATAGTGCCAG‑CAGTACTTTTGGCAAGG‑ATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAA‑‑Cccgc                                                                                                                                                  <  1:2144249/150‑1 (MQ=255)
      gCAGTAAATATGTTTTATTGCGTTACCGTTCATTCACAATACTGGAGCAATCCAGTATGTTCATTCTCTGGTATAGTGCCAG‑CAGTACTTTTGGCAAGG‑ATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAA‑‑Cccgcc                                                                                                                                                 >  1:3922444/1‑150 (MQ=255)
      gCAGTAAATATGTTTTATTGCGTTACCGTTCATTCACAATACTGGAGCAATCCAGTATGTTCATTCTCTGGTATAGTGCCAG‑CAGTACTTTTGGCAAGG‑ATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAA‑‑Cccgcc                                                                                                                                                 <  1:4091962/150‑1 (MQ=255)
      gCAGTAAATATGTTTTATTGCGTTACCGTTCATTCACAATACTGGAGCAATCCAGTATGTTCATTCTCTGGTATAGTGCCAG‑CAGTACTTTTGGCAAGG‑ATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAA‑‑Cccgcc                                                                                                                                                 <  1:3843263/150‑1 (MQ=255)
      gCAGTAAATATGTTTTATTGCGTTACCGTTCATTCACAATACTGGAGCAATCCAGTATGTTCATTCTCTGGTATAGTGCCAG‑CAGTACTTTTGGCAAGG‑ATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAA‑‑Cccgcc                                                                                                                                                 >  1:891479/1‑150 (MQ=255)
       cAGTAAATATGTTTTATTGCGTTACCGTTCATTCACAATACTGGAGCAATCCAGTATGTTCATTCTCTGGTATAGTGCCAG‑CAGTACTTTTGGCAAGG‑ATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAA‑‑Cccgccg                                                                                                                                                >  1:1724280/1‑150 (MQ=255)
          tAAATATGTTTTATTGCGTTACCGTTCATTCACAATACTGGAGCAATCCAGTATGTTCATTCTCTGGTATAGTGCCAG‑CAGTACTTTTGGCAAGG‑ATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAA‑‑CCCGCCGTTc                                                                                                                                             >  1:2082985/1‑150 (MQ=255)
          tAAATATGTTTTATTGCGTTACCGTTCATTCACAATACTGGAGCAATCCAGTATGTTCATTCTCTGGTATAGTGCCAG‑CAGTACTTTTGGCAAGG‑ATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAA‑‑CCCGCCGTTc                                                                                                                                             >  1:4158475/1‑150 (MQ=255)
             atatGTTTTATTGCGTTACCGTTCATTCACAATACTGGAGCAATCCAGTATGTTCATTCTCTGGTATAGTGCCAG‑CAGTACTTTTGGCAAGG‑ATTCGGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAA‑‑CCCGCCGTTCGCt                                                                                                                                          >  2:296076/1‑150 (MQ=255)
                 tttttATTGCTTTCCCGTTCATTCACAATACTGGAGAAATCAAGTATGTTCATTCTCTGTTATAGCGCCAG‑CATTATTTTGGGAAAGG‑ATCCAGCCTTCGTGAGGGGCATAAGAGCAAAAAAAAAAAAAAAAC‑‑CCCGCCGTTCGCtggtg                                                                                                                                      <  2:3696508/149‑1 (MQ=255)
                  ttttATTGCGTTACCGTTCATTCACAATACTGGAGCAATCCAGTATGTTCATTCTCTGGTATAGTGCCAG‑CAGTACTTTTGGCAAGG‑ATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAA‑‑CCCGCCGTTCGCtggtgg                                                                                                                                     >  2:1510437/1‑150 (MQ=255)
                   tttATTGCGTTACCGTTCATTCACAATACTGGAGCAATCCAGTATGTTCATTCTCTGGTATAGTGCCAG‑CAGTACTTTTGGCAAGG‑ATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAA‑‑CCCGCCGTTCGCTGGTGGa                                                                                                                                    >  2:398681/1‑150 (MQ=255)
                    ttATTGCGTTCCCTTTCATTCACAATACTGGAACAATCCAGTATTTTCATTCTCTGGTATAGTGCCAG‑CAGTATTTTTGGAAAGT‑ATTCAGACATGGTGAGGGGCGAAAGAGCCCAAAAAAAAAAAAAAC‑‑CCCGCCGTTCGCTGGTGGaa                                                                                                                                   <  2:774162/150‑1 (MQ=255)
                    ttATTGCGTTACCGTTCATTCACAATACTGGAGCAATCCAGTATGTTCATTCTCTGGTATAGTGCCAG‑CAGTACTTTTGGCAAGG‑ATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAA‑‑CCCGCCGTTCGCTGGCGGaa                                                                                                                                   >  1:3732083/1‑150 (MQ=255)
                     tATTGCGTTACCGTTCATTCACAATACTGGAGCAATCCAGTATGTTCATTCTCTGGTATAGTGCCAG‑CAGTACTTTTGGCAAGG‑ATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAA‑‑CCCGCCGTTCGCTGGTGGAAg                                                                                                                                  >  1:832177/1‑150 (MQ=255)
                     tATTGCGTTACCGTTCATTCACAATACTGGAGCAATCCAGTATGTTCATTCTCTGGTATAGTGCCAG‑CAGTACTTTTGGCAAGG‑ATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAA‑‑CCCGCCGTTCGCTGGTGGAAg                                                                                                                                  >  2:3020295/1‑150 (MQ=255)
                     tATTGCGTTACCGTTCATTCACAATACTGGAGCAATCCAGTATGTTCATTCTCTGGTATAGTGCCAG‑CAGTACTTTTGGCAAGG‑ATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAA‑‑CCCGCCGTTCGCTGGTGGAAg                                                                                                                                  >  1:1168390/1‑150 (MQ=255)
                      aTTGCGTTACCGTTCATTCACAATACTGGAGCAATCCAGTATGTTCATTCTCTGGTATAGTGCCAG‑CAGTACTTTTGGCAAGG‑ATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAA‑‑CCCGCCGTTCGCTGGTGGAAGc                                                                                                                                 >  2:3905504/1‑150 (MQ=255)
                          cGTTACCGTTCATTCACAATACTGGAGCAATCCAGTATGTTCATTCTCTGGTATAGTGCCAG‑CAGTACTTTTGGCAAGG‑ATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAA‑‑CCCGCCGTTCGCTGGTGGAAGCCGCa                                                                                                                             >  1:1916365/1‑150 (MQ=255)
                              aCCTTTCATTCAAAATACGGGACAAATCAATTATTTTCATTCTCTGGTATAGTGCCAG‑CAGTATTTTGGACAAGG‑ATTCAGCCATCGTGGGGGGGGAAAGAGCCAAAAAAAAAAAAAAAC‑‑CCCGCCGTTCGCTGGTGGAAGCCGCATTTa                                                                                                                         <  2:2156987/150‑1 (MQ=255)
                                 atacaatcaCAATGCGGGAGCAACCGAGTCTTTTCCTTCTCAGGTATAG‑CCCAGCCAGTAATTTTGCCAAGG‑ACCCAGACAGCGTGAGGGGCAAAAAAGCGCAAAAAAAAAAAAAAC‑‑CCCGCCGTTCGCTGGTGGAAGCCGCATTTAGcc                                                                                                                      <  2:2920687/144‑1 (MQ=255)
                                   tcattcaCAATACTGGAGCAATCCAGTATGTTCATTCTCTGGTATAGTGCCAG‑CAGTACTTTTGGCAAGG‑ATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAA‑‑CCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCaa                                                                                                                    >  2:817356/1‑150 (MQ=255)
                                     attcaCAATACTGGAGCAATCCAGTATGTTCATTCTCTGGTATAGTGCCAG‑CAGTACTTTTGGCAAGG‑ATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAA‑‑CCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAAtt                                                                                                                  >  2:2700755/1‑150 (MQ=255)
                                        cacaATACTGGAGCAATCCAGTATGTTCATTCTCTGGTATAGTGCCAG‑CAGTACTTTTGGCAAGG‑ATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAA‑‑CCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAg                                                                                                               >  1:4044063/1‑150 (MQ=255)
                                        cacaATACTGGAGCAATCCAGTATGTTCATTCTCTGGTATAGTGCCAG‑CAGTACTTTTGGCAAGG‑ATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAA‑‑CCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAg                                                                                                               >  1:3863315/1‑150 (MQ=255)
                                          caATACTGGAGCAATCCAGTATGTTCATTCTCTGGTATAGTGCCAG‑CAGTACTTTTGGCAAGG‑ATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAA‑‑CCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTg                                                                                                             >  1:1101953/1‑150 (MQ=255)
                                           aaTACTGGAGCAATCCAGTATGTTCATTCTCTGGTATAGTGCCAG‑CAGTACTTTTGGCAAGG‑ATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAA‑‑CCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGc                                                                                                            >  2:1938856/1‑150 (MQ=255)
                                           aaTACTGGAGCAATCCAGTATGTTCATTCTCTGGTATAGTGCCAG‑CAGTACTTTTGGCAAGG‑ATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAA‑‑CCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGc                                                                                                            >  2:3495494/1‑150 (MQ=255)
                                               cTGGAGCAATCCAGTATGTTCATTCTCTGGTATAGTGCCAG‑CAGTACTTTTGGCAAGG‑ATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAA‑‑CCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGaa                                                                                                        >  2:3851943/1‑150 (MQ=255)
                                               cTGGAGCAATCCAGTATGTTCATTCTCTGGTATAGTGCCAG‑CAGTACTTTTGGCAAGG‑ATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAA‑‑CCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGaa                                                                                                        >  1:3077025/1‑150 (MQ=255)
                                               cTGGAGCAATCCAGTATGTTCATTCTCTGGTATAGTGCCAG‑CAGTACTTTTGGCAAGG‑ATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAA‑‑CCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGaa                                                                                                        >  2:2364194/1‑150 (MQ=255)
                                                tGGAGCAATCCAGTATGTTCATTCTCTGGTATAGTGCCAG‑CAGTACTTTTGGCAAGG‑ATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAA‑‑CCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAAc                                                                                                       >  1:3165747/1‑150 (MQ=255)
                                                     cAATCCAGTATGTTCATTCTCTGGTATAGTGCCAG‑CAGTACTTTTGGCAAGG‑ATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAA‑‑CCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGc                                                                                                  >  2:1271635/1‑150 (MQ=255)
                                                      aaTCCAGTATGTTCATTCTCTGGTATAGTGCCAG‑CAGTACTTTTGGCAAGG‑ATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAA‑‑CCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGGGCTGAACGCAGCt                                                                                                 >  2:132490/1‑150 (MQ=255)
                                                       aTCCAGTATGTTCATTCTCTGGTATAGTGCCAG‑CAGTACTTTTGGCAAGG‑ATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAA‑‑CCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCtt                                                                                                >  2:2842430/1‑150 (MQ=255)
                                                       aTCCAGTATGTTCATTCTCTGGTATAGTGCCAG‑CAGTACTTTTGGCAAGG‑ATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAA‑‑CCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCtt                                                                                                >  2:2056591/1‑150 (MQ=255)
                                                         ccAGTATGTTCATTCTCTGGTATAGTGCCAG‑CAGTACTTTTGGCAAGG‑ATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAA‑‑CCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCg                                                                                              >  1:3263809/1‑150 (MQ=255)
                                                            ttATGTTCTTTCTTTGTTATAGGGCCAG‑CATTACTTTTGGAAAGG‑ATTCAGACATCGGGAGGGGGGAAAGACCAAAAAAAAAAAAAAAAC‑‑CCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCa                                                                                           <  2:1411485/149‑1 (MQ=255)
                                                              aTGTTCATTCTCTGGTATAGTGCCAG‑CAGTACTTTTGGCAAGG‑ATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAA‑‑CCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGc                                                                                         >  1:2492786/1‑150 (MQ=255)
                                                               tGTTCATTCTCTGGTATAGTGCCAG‑CAGTACTTTTGGCAAGG‑ATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAA‑‑CCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGcc                                                                                        >  2:453080/1‑150 (MQ=255)
                                                                  tCATTCTCTGGTATAGTGCCAG‑CAGTACTTTTGGCAAGG‑ATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAA‑‑CCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGa                                                                                     >  1:2040515/1‑150 (MQ=255)
                                                                   ctttCTCGGGTATAGGGCCAG‑CAGTTCTTTGGGCAGGG‑TTTCAGCCCCGGGGCGGGGCGAAAGGGCACAAAAAAAAAAAAACC‑‑CCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAg                                                                                    <  2:125527/148‑1 (MQ=255)
                                                                   cATTCTCTGGTATAGTGCCAG‑CAGTACTTTTGGCAAGG‑ATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAA‑‑CCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAg                                                                                    >  1:2911799/1‑150 (MQ=255)
                                                                    aTTCTCTGGTATAGTGCCAG‑CAGTACTTTTGGCAAGG‑ATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAA‑‑CCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGt                                                                                   >  2:2276421/1‑150 (MQ=255)
                                                                     ttCTCTGGTATAGTGCCAG‑CAGTACTTTTGGCAAGG‑ATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAA‑‑CCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGtt                                                                                  >  2:1716201/1‑150 (MQ=255)
                                                                      tctcTGGTATAGTGCCAG‑CAGTACTTTTGGCAAGG‑ATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAA‑‑CCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGttt                                                                                 >  2:2510004/1‑150 (MQ=255)
                                                                        tctGGTATAGTGCCAG‑CAGTACTTTTGGCAAGG‑ATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAA‑‑CCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGc                                                                               >  1:1248026/1‑150 (MQ=255)
                                                                          tGGTATAGTGCCAG‑CAGTACTTTTGGCAAGG‑ATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAA‑‑CCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGt                                                                             >  2:2205328/1‑150 (MQ=255)
                                                                            gTATAGTGCCAG‑CAGTACTTTTGGCAAGG‑ATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAA‑‑CCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGa                                                                           >  1:3766543/1‑150 (MQ=255)
                                                                             tataGTGCCAG‑CAGTACTTTTGGCAAGG‑ATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAA‑‑CCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGaa                                                                          >  1:1805815/1‑150 (MQ=255)
                                                                             tataGTGCCAG‑CAGTACTTTTGGCAAGG‑ATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAA‑‑CCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGaa                                                                          >  2:4063216/1‑150 (MQ=255)
                                                                                 gTGCCAG‑CAGTACTTTTGGCAAGG‑ATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAA‑‑CCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTgg                                                                      >  1:1586330/1‑150 (MQ=255)
                                                                                     cag‑cagTATTTTGGGAAAGG‑TTTCAGACATCGTGAGGGGCGAAAGAGCACAAAAAAAAAAAAAAC‑‑CCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGcgcg                                                                  <  1:788930/150‑1 (MQ=255)
                                                                                     cag‑cagTACTTTTGGCAAGG‑ATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAA‑‑CCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGcgcg                                                                  >  1:2953766/1‑150 (MQ=255)
                                                                                       g‑cagTACTTTTGGCAAGG‑ATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAA‑‑CCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTg                                                                >  1:656296/1‑150 (MQ=255)
                                                                                         cagTACTTTTGGCAAGG‑ATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAA‑‑CCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGa                                                               >  2:2437557/1‑150 (MQ=255)
                                                                                          cagtattttGGGAAGGAATTCAGAAATCGTGGGGGGGGAAAGAGCGCAAAAAAAAAAAAAAC‑‑CCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGa                                                               <  1:2000538/145‑1 (MQ=255)
                                                                                           attcTTTTGGGAAGGAATCCAGACTTGGTGGGGGGCGAAAGAGCGAAAAAAAAAAAAAACC‑‑CCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGaa                                                              <  2:3336970/147‑1 (MQ=255)
                                                                                                 ttGGCAAGG‑ATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAA‑‑CCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGGCa                                                       >  2:187672/1‑150 (MQ=255)
                                                                                                   ggCAAGG‑TTCCAGACATCGGGGGGGGCGAAAGGCCGAAAAAAAAAAAAAAAC‑‑CCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGGCAtt                                                     <  2:2285964/150‑1 (MQ=255)
                                                                                                   ggCAAGG‑ATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAA‑‑CCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGGCAtt                                                     >  2:1051351/1‑150 (MQ=255)
                                                                                                    gCAAGG‑ATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAA‑‑CCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGGCATTg                                                    >  1:868015/1‑150 (MQ=255)
                                                                                                       aGG‑ATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAA‑‑CCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGGCATTGCTc                                                 >  2:3788580/1‑150 (MQ=255)
                                                                                                        gg‑ATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAA‑‑CCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGGCATTGCTcc                                                >  1:243335/1‑150 (MQ=255)
                                                                                                           aTTCAGACATGGTGAGGGGCGAAAGAGCAAAAAAAAAAAAAAAAC‑‑CCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGGCATTGCTCCCa                                              <  2:1785927/150‑1 (MQ=255)
                                                                                                           aTTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAA‑‑CCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGGCATTGCTCCCa                                              >  2:3123858/1‑150 (MQ=255)
                                                                                                                 aaattgtggtgGGCAAAAGCGCCAAAAAAAAAAAAAAAC‑‑CCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGGCATTGCTCCCACCTCtt                                        <  2:3285846/145‑1 (MQ=255)
                                                                                                                  cATCGTGAGGGGTATAAGAGCCAAAAAAAAAAAAAAAC‑‑CCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGGCATTGCTCCCACCTCttt                                       <  1:3546405/150‑1 (MQ=255)
                                                                                                                  cATCGTGAGGGGGGAAAGAGCGAAAAAAAAAAAAAAAC‑‑CCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGGCATTGCTCCCACCTCttt                                       <  1:2735112/150‑1 (MQ=255)
                                                                                                                     ggTGAGGGGCGAAAGAGCGAAAAAAAAAAAAAAAC‑‑CCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGGCATTGCTCCCACCTCTTTTTa                                    <  1:1545605/149‑1 (MQ=255)
                                                                                                                     cGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAA‑‑CCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGGCATTGCTCCCACCTCTTTTTa                                    >  1:3327934/1‑150 (MQ=255)
                                                                                                                      ggtgggggCGAAAGGGCGCAAAAAAAAAAAAAAC‑‑CCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGGCATTGCTCCCACCTCTTTTTAt                                   <  2:3190467/145‑1 (MQ=255)
                                                                                                                      gggtggggCGAAAGAGCAAAAAAAAAAAAAAAAA‑‑CCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGGCATTGCTCCCACCTCTTTTTAt                                   <  1:702467/145‑1 (MQ=255)
                                                                                                                       tGATGGGCGAAAGAGCACAAAAAAAAAAAAAAC‑‑CCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGGCATTGCTCCCACCTCTTTTTATc                                  <  2:2639682/150‑1 (MQ=255)
                                                                                                                         gggggCGAAAGAGCAAAAAAAAAAAAAAAAC‑‑CCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGGCATTGCTCCCACCTCTTTTTATCgg                                <  2:3583178/148‑1 (MQ=255)
                                                                                                                          ggggCGTAAGAGCGAAAAAAAAAAAAAAAC‑‑CCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGGCATTGCTCCCACCTCTTTTTATCGGc                               <  1:4118455/149‑1 (MQ=255)
                                                                                                                              cgaaaGAGCGAAAAAAAAAAAAAAAC‑‑CCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGGCATTGCTCCCACCTCTTTTTATCGGCAttt                           <  1:3789150/147‑1 (MQ=255)
                                                                                                                                tAAGAGCGCAACAAAAAGAAAAAA‑‑CCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGGCATTGCTCCCACCTCTTTTTATCGGCATTTcc                         >  2:1463265/1‑150 (MQ=255)
                                                                                                                                tAAGAGCAAAAAAAAAAAAAAAAC‑‑CCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGTGTGAAGTGGCGCGTGAAGCGGGCATTGCTCCCACCTCTTTTTATCGGCATTTcc                         <  2:1674403/150‑1 (MQ=255)
                                                                                                                                caaaagCGCAAAAAAAAAAAAAAACCCCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGGCATTGCTCCCACCTCTTTTTATCGGCAttt                           <  2:4149018/146‑1 (MQ=255)
                                                                                                                                 aaGAGCGCAACAAAAAGAAAAAA‑‑CCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGGCATTGCTCCCACCTCTTTTTATCGGCATTTCcg                        >  1:742958/1‑150 (MQ=255)
                                                                                                                                   gagCGCAACAAAAAGAAAAAA‑‑CCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGGCATTGCTCCCACCTCTTTTTATCGGCATTTCcgcg                      >  2:532309/1‑150 (MQ=255)
                                                                                                                                         aacaaAAAGAAAAAA‑‑CCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGGCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAg                >  1:2587719/1‑150 (MQ=255)
                                                                                                                                            aaaaaGAAAAAA‑‑CCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGGCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACg             >  1:486849/1‑150 (MQ=255)
                                                                                                                                              aaaGAAAATC‑‑CCCGCCGTTCGCTGGGGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGGCATTGCTCCCCCCTCTTTTTATCGGCATTTCCGCGACGTAGACGaa           <  2:3866369/150‑1 (MQ=255)
                                                                                                                                                 gAAAAAA‑‑CCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGGCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTg        >  2:1648002/1‑150 (MQ=255)
                                                                                                                                                 aaaaaaa‑‑CCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGGCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTg        <  1:1075788/149‑1 (MQ=255)
                                                                                                                                                  aaaaac‑‑CCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGGCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTgg       <  2:278617/150‑1 (MQ=255)
                                                                                                                                                  aaaaac‑‑CCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGGCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTgg       <  1:2229634/150‑1 (MQ=255)
                                                                                                                                                   aaaac‑‑CCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGGCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTggg      <  2:1170182/150‑1 (MQ=255)
                                                                                                                                                    aaac‑‑CCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGGCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGt     <  1:9970/150‑1 (MQ=255)
                                                                                                                                                     aaa‑‑CCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGGCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGTc    >  1:3024953/1‑150 (MQ=255)
                                                                                                                                                     aaa‑‑CCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGGCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGTc    >  2:1189960/1‑150 (MQ=255)
                                                                                                                                                     aaa‑‑CCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGGCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGTc    >  1:1279071/1‑150 (MQ=255)
                                                                                                                                                     aaa‑‑CCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGGCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGTc    >  1:3495535/1‑150 (MQ=255)
                                                                                                                                                       |                                                                                                                                                       
AGAATTGCAGTAAATATGTTTTATTGCGTTACCGTTCATTCACAATACTGGAGCAATCCAGTATGTTCATTCTCTGGTATAGTGCCAG‑CAGTACTTTTGGCAAGG‑ATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAA‑‑CCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGGCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGTCTG  >  AP012306/3590218‑3590516

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑