BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AP012306	1,076,347	Δ1 bp	coding (931/1101 nt)	chaA ←	calcium/sodium:proton antiporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AP012306	1,076,345	0	A	.	100.0%	1422.6 / NA	286	coding (933/1101 nt)	chaA	calcium/sodium:proton antiporter
Reads supporting (aligned to +/- strand): ref base A (0/0); new base . (146/140); total (146/140)

Only 100 of 308 total aligned reads displayed.

AAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCACCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCATAAAGGCAATTAGCGTGACGACAGGTACGGTAAGCGAAATGGTTGCTAACACTGAACCAAAGAACAGATTCATCGCGCGCTGAACCTGGTTGTTCAACACTGCTTTTAATGCACCTAAACCTTCCGGCGACAGAATCAACAGTGCCA  >  AP012306/1076196‑1076490

aaaTAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCACCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCATa                                                                                                                                                   >  1:2797012/1‑150 (MQ=255)

  aTAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGGGGGGAAGGAGATATGGCACAGCACTAAAGAGGCCACCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCATaag                                                                                                                                                 >  2:2808011/1‑149 (MQ=255)

   tAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCACCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCATaagg                                                                                                                                                >  2:558435/1‑148 (MQ=255)

   tAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCACCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCATaagg                                                                                                                                                >  2:2011077/1‑148 (MQ=255)

    aGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCACCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCATaaggc                                                                                                                                               >  1:3035976/1‑147 (MQ=255)

    aGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCACCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCATaaggc                                                                                                                                               >  1:2187500/1‑147 (MQ=255)

        ggcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCACCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCAT‑AAGGCAAtt                                                                                                                                          <  2:180427/150‑1 (MQ=255)

              cAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCACCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCAT‑AAGGCAATTAGCGTg                                                                                                                                    <  1:1250754/150‑1 (MQ=255)

              cAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCACCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCAT‑AAGGCAATTAGCGTg                                                                                                                                    <  2:3603148/150‑1 (MQ=255)

                gTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCACCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCAT‑AAGGCAATTAGCGTgac                                                                                                                                  <  1:4079634/150‑1 (MQ=255)

                gTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCACCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCAT‑AAGGCAATTAGCGTgac                                                                                                                                  >  2:1342696/1‑150 (MQ=255)

                gTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCACCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCAT‑AAGGCAATTAGCGTgac                                                                                                                                  <  2:2576764/150‑1 (MQ=255)

                 tGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCACCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCAT‑AAGGCAATTAGCGTgacg                                                                                                                                 <  1:3537970/150‑1 (MQ=255)

                   ccAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCACCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCAT‑AAGGCAATTAGCGTgacgac                                                                                                                               >  2:3532907/1‑150 (MQ=255)

                   ccAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCACCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCAT‑AAGGCAATTAGCGTgacgac                                                                                                                               >  2:3415036/1‑150 (MQ=255)

                    cAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCACCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCAT‑AAGGCAATTAGCGTGACGACa                                                                                                                              >  1:4608450/1‑150 (MQ=255)

                     aGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCACCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCAT‑AAGGCAATTAGCGTGACGACAg                                                                                                                             <  1:3225555/150‑1 (MQ=255)

                       aTGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCACCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCAT‑AAGGCAATTAGCGTGACGACAGGt                                                                                                                           <  1:686077/150‑1 (MQ=255)

                       aTGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCACCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCAT‑AAGGCAATTAGCGTGACGACAGGt                                                                                                                           <  2:7265/150‑1 (MQ=255)

                         gcgctgcgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCACCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCAT‑AAGGCAATTAGCGTGACGACAGGTAc                                                                                                                         >  1:3843199/1‑150 (MQ=255)

                           gctgcgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCACCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCAT‑AAGGCAATTAGCGTGACGACAGGTACgg                                                                                                                       <  2:2045666/150‑1 (MQ=255)

                           gctgcgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCACCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCAT‑AAGGCAATTAGCGTGACGACAGGTACgg                                                                                                                       <  2:4590345/150‑1 (MQ=255)

                              gcgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCACCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCTTCAT‑AAGGCAATTAGCGTGACGACAGGTACGGTaa                                                                                                                    <  2:1008051/150‑1 (MQ=255)

                                 ccATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCACCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCAT‑AAGGCAATTAGCGTGACGACAGGTACGGTAAGCg                                                                                                                 <  1:52432/150‑1 (MQ=255)

                                 ccATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCACCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCAT‑AAGGCAATTAGCGTGACGACAGGTACGGTAAGCg                                                                                                                 <  2:614435/150‑1 (MQ=255)

                                   aTTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCACCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCAT‑AAGGCAATTAGCGTGACGACAGGTACGGTAAGCGaa                                                                                                               <  1:3647688/150‑1 (MQ=255)

                                    ttGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCACCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCAT‑AAGGCAATTAGCGTGACGACAGGTACGGTAAGCGaaa                                                                                                              <  1:476587/150‑1 (MQ=255)

                                      gAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCACCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCAT‑AAGGCAATTAGCGTGACGACAGGTACGGTAAGCGAAATg                                                                                                            <  1:2479251/150‑1 (MQ=255)

                                      gAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCACCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCAT‑AAGGCAATTAGCGTGACGACAGGTACGGTAAGCGAAATg                                                                                                            <  2:3848262/150‑1 (MQ=255)

                                      gAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCACCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCAT‑AAGGCAATTAGCGTGACGACAGGTACGGTAAGCGAAATg                                                                                                            <  2:2459678/150‑1 (MQ=255)

                                      gAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCACCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCAT‑AAGGCAATTAGCGTGACGACAGGTACGGTAAGCGAAATg                                                                                                            <  2:4043708/150‑1 (MQ=255)

                                        gCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCACCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCAT‑AAGGCAATTAGCGTGACGACAGGTACGGTAAGCGAAATGGt                                                                                                          <  2:1429920/150‑1 (MQ=255)

                                        gCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCACCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCAT‑AAGGCAATTAGCGTGACGACAGGTACGGTAAGCGAAATGGt                                                                                                          >  2:1045256/1‑150 (MQ=255)

                                        gCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCACCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCAT‑AAGGCAATTAGCGTGACGACAGGTACGGTAAGCGAAATGGt                                                                                                          >  2:4545750/1‑150 (MQ=255)

                                         cATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCACCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCAT‑AAGGCAATTAGCGTGACGACAGGTACGGTAAGCGAAATGGtt                                                                                                         >  1:1292415/1‑150 (MQ=255)

                                           tGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCACCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCAT‑AAGGCAATTAGCGTGACGACAGGTACGGTAAGCGAAATGGTTGc                                                                                                       <  1:953620/150‑1 (MQ=255)

                                               aGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCACCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCAT‑AAGGCAATTAGCGTGACGACAGGTACGGTAAGCGAAATGGTTGCTAac                                                                                                   >  1:1548495/1‑150 (MQ=255)

                                                gTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCACCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCAT‑AAGGCAATTAGCGTGACGACAGGTACGGTAAGCGAAATGGTTGCTAaca                                                                                                  >  1:4294109/1‑150 (MQ=255)

                                                    gTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCACCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCAT‑AAGGCAATTAGCGTGACGACAGGTACGGTAAGCGAAATGGTTGCTAACACTGa                                                                                              <  1:139455/150‑1 (MQ=255)

                                                      ccGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCACCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCAT‑AAGGCAATTAGCGTGACGACAGGTACGGTAAGCGAAATGGTTGCTAACACTGAAc                                                                                            <  2:1738597/150‑1 (MQ=255)

                                                         gTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCACCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCAT‑AAGGCAATTAGCGTGACGACAGGTACGGTAAGCGAAATGGTTGCTAACACTGAACCaa                                                                                         >  1:902940/1‑150 (MQ=255)

                                                         gTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCACCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCAT‑AAGGCAATTAGCGTGACGACAGGTACGGTAAGCGAAATGGTTGCTAACACTGAACCaa                                                                                         <  1:3962907/150‑1 (MQ=255)

                                                          tGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCACCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCAT‑AAGGCAATTAGCGTGACGACAGGTACGGTAAGCGAAATGGTTGCTAACACTGAACCaaa                                                                                        <  1:2357654/150‑1 (MQ=255)

                                                           ggAGAAGGAGATATGGCACAGCACTAAAGAGGCCACCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCAT‑AAGGCAATTAGCGTGACGACAGGTACGGTAAGCGAAATGGTTGCTAACACTGAACCAAAg                                                                                       <  1:2332616/150‑1 (MQ=255)

                                                              gaAGGAGATATGGCACAGCACTAAAGAGGCCACCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCAT‑AAGGCAATTAGCGTGACGACAGGTACGGTAAGCGAAATGGTTGCTAACACTGAACCAAAGAAc                                                                                    <  2:2256518/150‑1 (MQ=255)

                                                              gaAGGAGATATGGCACAGCACTAAAGAGGCCACCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCAT‑AAGGCAATTAGCGTGACGACAGGTACGGTAAGCGAAATGGTTGCTAACACTGAACCAAAGAAc                                                                                    <  1:2537367/150‑1 (MQ=255)

                                                              gaAGGAGATATGGCACAGCACTAAAGAGGCCACCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCAT‑AAGGCAATTAGCGTGACGACAGGTACGGTAAGCGAAATGGTTGCTAACACTGAACCAAAGAAc                                                                                    <  1:2023899/150‑1 (MQ=255)

                                                              gaAGGAGATATGGCACAGCACTAAAGAGGCCACCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCAT‑AAGGCAATTAGCGTGACGACAGGTACGGTAAGCGAAATGGTTGCTAACACTGAACCAAAGAAc                                                                                    >  1:4531557/1‑150 (MQ=255)

                                                               aaGGAGATATGGCACAGCACTAAAGAGGCCACCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCAT‑AAGGCAATTAGCGTGACGACAGGTACGGTAAGCGAAATGGTTGCTAACACTGAACCAAAGAACa                                                                                   >  1:578181/1‑150 (MQ=255)

                                                               aaGGAGATATGGCACAGCACTAAAGAGGCCACCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCAT‑AAGGCAATTAGCGTGACGACAGGTACGGTAAGCGAAATGGTTGCTAACACTGAACCAAAGAACa                                                                                   <  2:1466486/150‑1 (MQ=255)

                                                               aaGGAGATATGGCACAGCACTAAAGAGGCCACCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCAT‑AAGGCAATTAGCGTGACGACAGGTACGGTAAGCGAAATGGTTGCTAACACTGAACCAAAGAACa                                                                                   >  1:2834853/1‑150 (MQ=255)

                                                               aaGGAGATATGGCACAGCACTAAAGAGGCCACCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCAT‑AAGGCAATTAGCGTGACGACAGGTACGGTAAGCGAAATGGTTGCTAACACTGAACCAAAGAACa                                                                                   >  1:1065715/1‑150 (MQ=255)

                                                                   agaTATGGCACAGCACTAAAGAGGCCACCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCAT‑AAGGCAATTAGCGTGACGACAGGTACGGTAAGCGAAATGGTTGCTAACACTGAACCAAAGAACAGAtt                                                                               >  1:4211482/1‑150 (MQ=255)

                                                                   agaTATGGCACAGCACTAAAGAGGCCACCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCAT‑AAGGCAATTAGCGTGACGACAGGTACGGTAAGCGAAATGGTTGCTAACACTGAACCAAAGAACAGAtt                                                                               <  1:3395706/150‑1 (MQ=255)

                                                                            acaGCACTAAAGAGGCCACCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCAT‑AAGGCAATTAGCGTGACGACAGGTACGGTAAGCGAAATGGTTGCTAACACTGAACCAAAGAACAGATTCATcgcgcg                                                                      >  2:275753/1‑150 (MQ=255)

                                                                               gCACTAAAGAGGCCACCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCAT‑AAGGCAATTAGCGTGACGACAGGTACGGTAAGCGAAATGGTTGCTAACACTGAACCAAAGAACAGATTCATCGCGCGCTg                                                                   >  1:2589873/1‑150 (MQ=255)

                                                                                cACTAAAGAGGCCACCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCAT‑AAGGCAATTAGCGTGACGACAGGTACGGTAAGCGAAATGGTTGCTAACACTGAACCAAAGAACAGATTCATCGCGCGCTGa                                                                  >  1:3593858/1‑150 (MQ=255)

                                                                                 aCTAAAGAGGCCACCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCAT‑AAGGCAATTAGCGTGACGACAGGTACGGTAAGCGAAATGGTTGCTAACACTGAACCAAAGAACAGATTCATCGCGCGCTGaa                                                                 >  2:2636196/1‑150 (MQ=255)

                                                                                   tAAAGAGGCCACCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCAT‑AAGGCAATTAGCGTGACGACAGGTACGGTAAGCGAAATGGTTGCTAACACTGAACCAAAGAACAGATTCATCGCGTGCTGAAcc                                                               <  1:2271121/150‑1 (MQ=255)

                                                                                      agagGCCACCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCAT‑AAGGCAATTAGCGTGACGACAGGTACGGTAAGCGAAATGGTTGCTAACACTGAACCAAAGAACAGATTCATCGCGCGCTGAACCTgg                                                            >  1:918484/1‑150 (MQ=255)

                                                                                       gagGCCACCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCAT‑AAGGCAATTAGCGTGACGACAGGTACGGTAAGCGAAATGGTTGCTAACACTGAACCAAAGAACAGATTCATCGCGCGCTGAACCTGgt                                                           <  1:3919895/150‑1 (MQ=255)

                                                                                       gagGCCACCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCAT‑AAGGCAATTAGCGTGACGACAGGTACGGTAAGCGAAATGGTTGCTAACACTGAACCAAAGAACAGATTCATCGCGCGCTGAACCTGgt                                                           >  2:1548749/1‑150 (MQ=255)

                                                                                        agGCCACCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCAT‑AAGGCAATTAGCGTGACGACAGGTACGGTAAGCGAAATGGTTGCTAACACTGAACCAAAGAACAGATTCATCGCGCGCTGAACCTGgtt                                                          >  2:3924026/1‑150 (MQ=255)

                                                                                         ggCCACCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCAT‑AAGGCAATTAGCGTGACGACAGGTACGGTAAGCGAAATGGTTGCTAACACTGAACCAAAGAACAGATTCATCGCGCGCTGAACCTGgttg                                                         <  1:4372888/150‑1 (MQ=255)

                                                                                          gCCACCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCAT‑AAGGCAATTAGCGTGACGACAGGTACGGTAAGCGAAATGGTTGCTAACACTGAACCAAAGAACAGATTCATCGCGCGCTGAACCTGgttgt                                                        <  1:3483135/150‑1 (MQ=255)

                                                                                           ccaccaTCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCAT‑AAGGCAATTAGCGTGACGACAGGTACGGTAAGCGAAATGGTTGCTAACACTGAACCAAAGAACAGATTCATCGCGCGCTGAACCTGgttgtt                                                       >  2:2535547/1‑150 (MQ=255)

                                                                                           ccaccaTCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCAT‑AAGGCAATTAGCGTGACGACAGGTACGGTAAGCGAAATGGTTGCTAACACTGAACCAAAGAACAGATTCATCGCGCGCTGAACCTGgttgtt                                                       <  1:3746775/150‑1 (MQ=255)

                                                                                               caTCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCAT‑AAGGCAATTAGCGTGACGACAGGTACGGTAAGCGAAATGGTTGCTAACACTGAACCAAAGAACAGATTCATCGCGCGCTGAACCTGGTTGTTCAac                                                   >  1:4017017/1‑150 (MQ=255)

                                                                                                aTCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCAT‑AAGGCAATTAGCGTGACGACAGGTACGGTAAGCGAAATGGTTGCTAACACTGAACCAAAGAACAGATTCATCGCGCGCTGAACCTGGTTGTTCAaca                                                  >  2:4624310/1‑150 (MQ=255)

                                                                                                  caccacCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCAT‑AAGGCAATTAGCGTGACGACAGGTACGGTAAGCGAAATGGTTGCTAACACTGAACCAAAGAACAGATTCATCGCGCGCTGAACCTGGTTGTTCAACACt                                                <  1:1826830/150‑1 (MQ=255)

                                                                                                   accaccATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCAT‑AAGGCAATTAGCGTGACGACAGGTACGGTAAGCGAAATGGTTGCTAACACTGAACCAAAGAACAGATTCATCGCGCGCTGAACCTGGTTGTTCAACACTg                                               >  2:2113592/1‑150 (MQ=255)

                                                                                                   accaccATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCAT‑AAGGCAATTAGCGTGACGACAGGTACGGTAAGCGAAATGGTTGCTAACACTGAACCAAAGAACAGATTCATCGCGCGCTGAACCTGGTTGTTCAACACTg                                               <  1:3282004/150‑1 (MQ=255)

                                                                                                    ccaccaTTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCAT‑AAGGCAATTAGCGTGACGACAGGTACGGTAAGCGAAATGGTTGCTAACACTGAACCAAAGAACAGATTCATCGCGCGCTGAACCTGGTTGTTCAACACTGc                                              <  1:1664445/150‑1 (MQ=255)

                                                                                                    ccaccaTTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCAT‑AAGGCAATTAGCGTGACGACAGGTACGGTAAGCGAAATGGTTGCTAACACTGAACCAAAGAACAGATTCATCGCGCGCTGAACCTGGTTGTTCAACACTGc                                              >  2:1660168/1‑150 (MQ=255)

                                                                                                    ccaccaTTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCAT‑AAGGCAATTAGCGTGACGACAGGTACGGTAAGCGAAATGGTTGCTAACACTGAACCAAAGAACAGATTCATCGCGCGCTGAACCTGGTTGTTCAACACTGc                                              <  2:4299984/150‑1 (MQ=255)

                                                                                                      accaTTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCAT‑AAGGCAATTAGCGTGACGACAGGTACGGTAAGCGAAATGGTTGCTAACACTGAACCAAAGAACAGATTCATCGCGCGCTGAACCTGGTTGTTCAACACTGCtt                                            >  1:2728510/1‑150 (MQ=255)

                                                                                                        caTTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCAT‑AAGGCAATTAGCGTGACGACAGGTACGGTAAGCGAAATGGTTGCTAACACTGAACCAAAGAACAGATTCATCGCGCGCTGAACCTGGTTGTTCAACACTGCtttt                                          <  2:1293501/150‑1 (MQ=255)

                                                                                                        caTTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCAT‑AAGGCAATTAGCGTGACGACAGGTACGGTAAGCGAAATGGTTGCTAACACTGAACCAAAGAACAGATTCATCGCGCGCTGAACCTGGTTGTTCAACACTGCtttt                                          <  1:651524/150‑1 (MQ=255)

                                                                                                         aTTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCAT‑AAGGCAATTAGCGTGACGACAGGTACGGTAAGCGAAATGGTTGCTAACACTGAACCAAAGAACAGATTCATCGCGCGCTGAACCTGGTTGTTCAACACTGCTTTTa                                         >  1:1481767/1‑150 (MQ=255)

                                                                                                         aTTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCAT‑AAGGCAATTAGCGTGACGACAGGTACGGTAAGCGAAATGGTTGCTAACACTGAACCAAAGAACAGATTCATCGCGCGCTGAACCTGGTTGTTCAACACTGCTTTTa                                         >  2:2359298/1‑150 (MQ=255)

                                                                                                         aTTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCAT‑AAGGCAATTAGCGTGACGACAGGTACGGTAAGCGAAATGGTTGCTAACACTGAACCAAAGAACAGATTCATCGCGCGCTGAACCTGGTTGTTCAACACTGCTTTTa                                         >  1:4425105/1‑150 (MQ=255)

                                                                                                          tttCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCAT‑AAGGCAATTAGCGTGACGACAGGTACGGTAAGCGAAATGGTTGCTAACACTGAACCAAAGAACAGATTCATCGCGCGCTGAACCTGGTTGTTCAACACTGCTTTTaa                                        <  1:2428041/150‑1 (MQ=255)

                                                                                                            tCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCAT‑AAGGCAATTAGCGTGACGACAGGTACGGTAAGCGAAATGGTTGCTAACACTGAACCAAAGAACAGATTCATCGCGCGCTGAACCTGGTTGTTCAACACTGCTTTTAATg                                      <  1:1358422/150‑1 (MQ=255)

                                                                                                              tGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCAT‑AAGGCAATTAGCGTGACGACAGGTACGGTAAGCGAAATGGTTGCTAACACTGAACCAAAGAACAGATTCATCGCGCGCTGAACCTGGTTGTTCAACACTGCTTTTAATGCa                                    <  2:3089383/150‑1 (MQ=255)

                                                                                                                 cgcACCAAGTGCAAACTGCAATTCGTTACCCGTCAT‑AAGGCAATTAGCGTGACGACAGGTACGGTAAGCGAAATGGTTGCTAACACTGAACCAAAGAACAGATTCATCGCGCGCTGAACCTGGTTGTTCAACACTGCTTTTAATGCACCt                                 <  2:3758548/150‑1 (MQ=255)

                                                                                                                  gcACCAAGTGCAAACTGCAATTCGTTACCCGTCAT‑AAGGCAATTAGCGTGACGACAGGTACGGTAAGCGAAATGGTTGCTAACACTGAACCAAAGAACAGATTCATCGCGCGCTGAACCTGGTTGTTCAACACTGCTTTTAATGCACCTa                                <  2:3500341/150‑1 (MQ=255)

                                                                                                                    aCCAAGTGCAAACTGCAATTCGTTACCCGTCAT‑AAGGCAATTAGCGTGACGACAGGTACGGTAAGCGAAATGGTTGCTAACACTGAACCAAAGAACAGATTCATCGCGCGCTGAACCTGGTTGTTCAACACTGCTTTTAATGCACCTaaa                              <  2:794404/150‑1 (MQ=255)

                                                                                                                     ccAAGTGCAAACTGCAATTCGTTACCAGTCAT‑AAGGCAATTAGCGTGACGACAGGTACGGTAAGCGAAATGGTTGCTAACACTGAACCAAAGAACAGATTCATCGCGCGCTGAACCTGGTTGTTCAACACTGCTTTTAATGCACCTAAAc                             <  2:2925480/150‑1 (MQ=255)

                                                                                                                       aaGTGCAAACTGCAATTCGTTACCCGTCAT‑AAGGCAATTAGCGTGACGACAGGTACGGTAAGCGAAATGGTTGCTAACACTGAACCAAAGAACAGATTCATCGCGCGCTGAACCTGGTTGTTCAACACTGCTTTTAATGCACCTAAACCt                           >  2:2413996/1‑150 (MQ=255)

                                                                                                                          tGCAAACTGCAATTCGTTACCCGTCAT‑AAGGCAATTAGCGTGACGACAGGTACGGTAAGCGAAATGGTTGCTAACACTGAACCAAAGAACAGATTCATCGCGCGCTGAACCTGGTTGTTCAACACTGCTTTTAATGCACCTAAACCTTcc                        <  1:4405444/150‑1 (MQ=255)

                                                                                                                              aaCTGCAATTCGTTACCCGTCAT‑AAGGCAATTAGCGTGACGACAGGTACGGTAAGCGAAATGGTTGCTAACACTGAACCAAAGAACAGATTCATCGCGCGCTGAACCTGGTTGTTCAACACTGCTTTTAATGCACCTAAACCTTCCGGCg                    >  1:1925300/1‑150 (MQ=255)

                                                                                                                                 tGCAATTCGTTACCCGTCAT‑AAGGCAATTAGCGTGACGACAGGTACGGTAAGCGAAATGGTTGCTAACACTGAACCAAAGAACAGATTCATCGCGCGCTGAACCTGGTTGTTCAACACTGCTTTTAATGCACCTAAACCTTCCGGCGACa                 <  2:4207927/150‑1 (MQ=255)

                                                                                                                                  gCAATTCGTTACCCGTCAT‑AAGGCAATTAGCGTGACGACAGGTACGGTAAGCGAAATGGTTGCTAACACTGAACCAAAGAACAGATTCATCGCGCGCTGAACCTGGTTGTTCAACACTGCTTTTAATGCACCTAAACCTTCCGGCGACAg                >  2:2898538/1‑150 (MQ=255)

                                                                                                                                  gCAATTCGTTACCCGTCAT‑AAGGCAATTAGCGTGACGACAGGTACGGTAAGCGAAATGGTTGCTAACACTGAACCAAAGAACAGATTCATCGCGCGCTGAACCTGGTTGTTCAACACTGCTTTTAATGCACCTAAACCTTCCGGCGACAg                <  2:1856985/150‑1 (MQ=255)

                                                                                                                                   cAATTCGTTACCCGTCAT‑AAGGCAATTAGCGTGACGACAGGTACGGTAAGCGAAATGGTTGCTAACACTGAACCAAAGAACAGATTCATCGCGCGCTGAACCTGGTTGTTCAACACTGCTTTTAATGCACCTAAACCTTCCGGCGACAGa               <  1:1642183/150‑1 (MQ=255)

                                                                                                                                    aaTTCGTTACCCGTCAT‑AAGGCAATTAGCGTGACGACAGGTACGGTAAGCGAAATGGTTGCTAACACTGAACCAAAGAACAGATTCATCGCGCGCTGAACCTGGTTGTTCAACACTGCTTTTAATGCACCTAAACCTTCCGGCGACAGaa              <  2:387162/150‑1 (MQ=255)

                                                                                                                                       tCGTTACCCGTCAT‑AAGGCAATTAGCGTGACGACAGGTACGGTAAGCGAAATGGTTGCTAACACTGAACCAAAGAACAGATTCATCGCGCGCTGAACCTGGTTGTTCAACACTGCTTTTAATGCACCTAAACCTTCCGGCGACAGAATCa           <  2:4365638/150‑1 (MQ=255)

                                                                                                                                           tACCCGTCAT‑AAGGCAATTAGCGTGACGACAGGTACGGTAAGCGAAATGGTTGCTAACACTGAACCAAAGAACAGATTCATCGCGCGCTGAACCTGGTTGTTCAACACTGCTTTTAATGCACCTAAACCTTCCGGCGACAGAATCAACAg       >  2:3167432/1‑150 (MQ=255)

                                                                                                                                             cccGTCAT‑AAGGCAATTAGCGTGACGACAGGTACGTTAACCGAAATGGTTGCTAACACTGAACCAAAGAACAGATTCATCGCGCGCTGAACCTGGTTGTTCAACACTGCTTTTAATGCACCTAAACCTTCCGGCTACAGAATCAACAGTg     >  1:307694/1‑150 (MQ=255)

                                                                                                                                               cGTCAT‑AAGGCAATTAGCGTGACGACAGGTACGGTAAGCGAAATGGTTGCTAACACTGAACCAAAGAACAGATTCATCGCGCGCTGAACCTGGTTGTTCAACACTGCTTTTAATGCACCTAAACCTTCCGGCGACAGAATCAACAGTGcc   >  2:1271912/1‑150 (MQ=255)

AAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCACCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCATAAAGGCAATTAGCGTGACGACAGGTACGGTAAGCGAAATGGTTGCTAACACTGAACCAAAGAACAGATTCATCGCGCGCTGAACCTGGTTGTTCAACACTGCTTTTAATGCACCTAAACCTTCCGGCGACAGAATCAACAGTGCCA  >  AP012306/1076196‑1076490

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`