Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123061,421,1470TG25.4% 513.9 / 13.0 244T79P (ACC→CCC) ydhBpredicted DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (98/84);  new base G (62/0);  total (160/84)
Fisher's exact test for biased strand distribution p-value = 3.02e-14
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 300 total aligned reads displayed.

TTCCTGAAAGACAAGAAGTTCGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTT‑GA‑GAAACCACGCTCCAGCAGCGGTCAGCTCCACATCACGGTGCCGTCGTTCAAAGAGCGGCACCGCCAGCCACTCTTCCAGCTGACGCACGGTATAGCTGACCGCAGAAGGAA  >  AP012306/1420998‑1421296
                                                                                                                                                     |                                                                                                                                                       
ttCCTGAAAGACAAGAAGTTCGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGt                                                                                                                                                         <  2:92704/150‑1 (MQ=255)
  ccTGAAAGACAAGAAGTTCGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTgg                                                                                                                                                       >  1:20664/1‑149 (MQ=255)
     gAAAGACAAGAAGTTCGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGATCt                                                                                                                                                    >  1:1616113/1‑150 (MQ=255)
      aaaGACAAGAAGTTCGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGAAAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTggcctg                                                                                                                                                   >  2:2328646/1‑145 (MQ=255)
       aaGACAAGAAGTTCGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGATCTGc                                                                                                                                                  >  1:2916764/1‑150 (MQ=255)
         gACAAGAAGTTCGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGATCTGcct                                                                                                                                                >  2:336657/1‑148 (MQ=255)
           cAAGAAGTTCGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGATCTGCAttt                                                                                                                                              >  2:1713140/1‑150 (MQ=255)
            aagaagTTCGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCAtttt                                                                                                                                             <  2:1042120/150‑1 (MQ=255)
            aagaagTTCGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGGTTTGCAtttt                                                                                                                                             >  2:3071241/1‑150 (MQ=255)
              gaagTTCGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCAtttttt                                                                                                                                           <  2:1314991/150‑1 (MQ=255)
                agTTCGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTg                                                                                                                                         <  1:892632/150‑1 (MQ=255)
                agTTCGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTg                                                                                                                                         >  1:4468118/1‑150 (MQ=255)
                agTTCGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGATCTGCAGTTTTTTg                                                                                                                                         >  1:2619705/1‑150 (MQ=255)
                  ttCGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGac                                                                                                                                       <  2:384864/150‑1 (MQ=255)
                    cGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGGTCTGCCttttttttgcaa                                                                                                                                     >  2:1270721/1‑145 (MQ=255)
                     gACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGGTCTGCCTTTTTTTTacaac                                                                                                                                    >  2:1422882/1‑150 (MQ=255)
                     gACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTTacaaa                                                                                                                                    >  2:2573307/1‑149 (MQ=255)
                       catcatCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACag                                                                                                                                  <  1:4231922/150‑1 (MQ=255)
                        atcatcAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACaga                                                                                                                                 <  2:4036778/150‑1 (MQ=255)
                        atcatcAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACaga                                                                                                                                 >  1:95668/1‑150 (MQ=255)
                        atcatcAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACaga                                                                                                                                 <  1:2326153/150‑1 (MQ=255)
                          catcaAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGATCTGCATTTTTTTTTCAACagaga                                                                                                                               >  2:1081279/1‑149 (MQ=255)
                           atcaAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAgcg                                                                                                                              >  1:1176314/1‑150 (MQ=255)
                           atcaAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAgcg                                                                                                                              <  2:4322347/150‑1 (MQ=255)
                              aaaaTGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGccc                                                                                                                           >  1:3656008/1‑150 (MQ=255)
                               aaaTGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTTACAACAGAGCGCCct                                                                                                                          >  2:3127186/1‑150 (MQ=255)
                               aaaTGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGCCct                                                                                                                          <  1:2823126/150‑1 (MQ=255)
                               aaaTGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGGTTTGCATTTTTTTTTCAACAAAGCGcccc                                                                                                                          >  2:4535344/1‑149 (MQ=255)
                                 aTGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCCTTTTTTTTACAAAAGAGCGCCcttc                                                                                                                        >  2:875634/1‑150 (MQ=255)
                                  tGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGGTCTGCATTTTTTTTACAACAGAGCGCCcctct                                                                                                                       >  2:4067180/1‑150 (MQ=255)
                                    gCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGCCCTTCttt                                                                                                                     <  2:1684514/150‑1 (MQ=255)
                                     cGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTT‑g                                                                                                                   >  1:1556824/1‑150 (MQ=255)
                                      gATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGGTCTGCATTTTTTTTACAACAGAGCGCCCTTCTTT‑ga                                                                                                                  >  1:1025790/1‑150 (MQ=255)
                                      gATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATTTGCCTTTTTTTGACAACAAAGCGCCCTTCTTT‑gt                                                                                                                  >  2:3974969/1‑149 (MQ=255)
                                      gATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGGGCTGGATTTTTTTGACAAAAGAGGGGCCGTCTTT‑ga                                                                                                                  >  2:2753435/1‑150 (MQ=255)
                                           aaaTCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGATCTGCATTTTTTTTACAACAGAGCGCCCTTCTTT‑GA‑GAAAc                                                                                                            >  2:1616477/1‑150 (MQ=255)
                                            aaTCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTT‑GA‑GAAAcc                                                                                                           <  2:4278173/150‑1 (MQ=255)
                                            aaTCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTT‑GA‑GAAAcc                                                                                                           <  2:4202893/150‑1 (MQ=255)
                                                aaCGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGCTCTTGCTTTTTTTTCCAACAATGAGCCCCTCTTT‑GATGAAAcccgc                                                                                                        >  1:268827/1‑147 (MQ=255)
                                                 aCGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTT‑GA‑GAAACCACGCt                                                                                                      >  1:1190516/1‑150 (MQ=255)
                                                 aCGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGCGATCTGCATTTTTTTGACAACAGAGCGCCCCTCTTT‑GA‑GAAACCACGCt                                                                                                      >  1:3512509/1‑150 (MQ=255)
                                                   gATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTTACAACAGAGCGCCCCTCTTT‑TA‑GAAACCACGcccc                                                                                                    >  2:4423923/1‑150 (MQ=255)
                                                   gATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGGCAACAAAGCGCCCCTCTTT‑GC‑GAAAACCCGcccc                                                                                                    >  2:1791164/1‑150 (MQ=255)
                                                    atcatcTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTT‑GA‑GAAACCACGCTCca                                                                                                   <  2:2477664/150‑1 (MQ=255)
                                                    accatcTGCCATGGACGTTCTGCCGTGACAATTTTATGCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGATGGCGGGTGATCTGCATTTTTTTGACAGCAGAGCGCGCTTCTTT‑GA‑GAAACCACGGTCca                                                                                                   <  2:1978507/148‑1 (MQ=255)
                                                     tcatcTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTT‑TA‑GAAACCCCGCTcccg                                                                                                  >  1:121011/1‑148 (MQ=255)
                                                      catcTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGATCTGCCTTTTTTTTACAACAGAGCGCCCCTCTTT‑GA‑GAAACCACGCTCcagc                                                                                                 >  2:599346/1‑150 (MQ=255)
                                                          tGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTTACAACAGAGCGCCCTTCCTT‑GA‑GAAACCACGCTCCAGCAGCg                                                                                             >  2:2649763/1‑150 (MQ=255)
                                                          tGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGGTCTTCCTTTTTTTGACAACAGAGCGCCCTTCTTT‑TA‑GAAACCACGCTCCAGCAGCg                                                                                             >  2:514301/1‑150 (MQ=255)
                                                           gCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTT‑GA‑GAAACCACGCTCCAGCAGCgg                                                                                            >  2:2926696/1‑150 (MQ=255)
                                                              gtgtACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTT‑GA‑GAAACCACGCTCCAGCAGCGGTCa                                                                                         >  1:3187523/1‑150 (MQ=255)
                                                              gtgtACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTGCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTT‑GA‑GAAACCACGCTCCAGCAGCGGTCa                                                                                         <  1:2108076/150‑1 (MQ=255)
                                                                 tACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTTACAACAGAGCGCCCTTCTTT‑GA‑GAAACCACGCTCCAGCAGCGGTCAGCt                                                                                      >  1:142265/1‑150 (MQ=255)
                                                                       cTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTT‑GA‑GAAACCACGCTCCAGCAGCGGTCAGCTCCACAt                                                                                <  1:465332/150‑1 (MQ=255)
                                                                       cTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGGTCTGCATTTTTTTTACAACAGAGCGCCCTTCTTT‑TA‑GAAACCACGCTCCAGCAGCGGGCAGCTCCACAt                                                                                >  2:3336529/1‑150 (MQ=255)
                                                                         ggCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTT‑GA‑GAAACCACGCTCCAGCAGCGGTCAGCTCCACATCa                                                                              <  1:1886128/150‑1 (MQ=255)
                                                                          gCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGATCTGCATTTTTTTTTCAACAGAGGGCCCTTCTTT‑TT‑GGAACCACCCCCCAGCCTCGGGCGGCTCCACATcca                                                                             >  2:2097072/1‑148 (MQ=255)
                                                                            cTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTT‑GA‑GAAACCACGCTCCAGCAGCGGTCAGCTCCACATCACgg                                                                           >  1:3006543/1‑150 (MQ=255)
                                                                             tGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTT‑GA‑GAAACCACGCTCCAGCAGCGGTCAGCTCCACATCACGGt                                                                          <  1:3802018/150‑1 (MQ=255)
                                                                             tGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGGTCTGCATTTTTTTGACAACAGAGCGCCCTTCTTT‑GA‑GAAACCACGCTCCAGCAGCGGTCAGCTCCACATCACGGt                                                                          >  1:805902/1‑150 (MQ=255)
                                                                             tGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGAGCTGAAATTTTTTTAGAACCGAGCGCCCTTCTTT‑GG‑GGAAACCCGCTCCTCCACCGGACACGTCCACACCTCGGt                                                                          >  2:150553/1‑150 (MQ=255)
                                                                               aCAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTT‑GA‑GAAACCACGCTCCAGCAGCGGTCAGCTCCACATCACGGTGc                                                                        >  2:3669071/1‑150 (MQ=255)
                                                                               aCAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTT‑GA‑GAAACCACGCTCCAGCAGCGGTCAGCTCCACATCACCGTGc                                                                        >  1:4096637/1‑150 (MQ=255)
                                                                                   tattatCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTT‑GA‑GAAACCACGCTCCAGCAGCGGTCAGCTCCCCATCACGGTGCcgtc                                                                    >  1:3910516/1‑150 (MQ=255)
                                                                                    attatCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTTACAACAGAGCGCCCTTCTTT‑GA‑GAAACCACGCTCCAGCAGCGGTCAGCTCCACATCACCGTGCcgtcg                                                                   >  1:4528694/1‑150 (MQ=255)
                                                                                     ttatCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTT‑GA‑GAAACCACGCTCCAGCAGCGGTCAGCTCCACATCACGGTGCcgtcgt                                                                  >  1:2514852/1‑150 (MQ=255)
                                                                                       atCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCCTTTTTTTGACAACAGAGCGCCCTTCTTT‑GA‑GAAACCACGCTCCAGCAGCGGTCAGCTCCACATCACGGTGCCGTCGTTc                                                                >  1:4404334/1‑150 (MQ=255)
                                                                                       atCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGATTTGCCTTTTTTTTTCAAAAGAGCGCCCTTCTTT‑TA‑GAAACCACGCCCCAACAGGGGTTAGCTCCACATCACGGGGCCGTCGTTc                                                                >  2:2747279/1‑150 (MQ=255)
                                                                                         ccACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAAAGCGCCCTTCTTT‑GA‑GAAACCACGCCCCAGCAGCGGGCAGCTCCACATCACCGTGCCGGCGTTCaa                                                              >  2:183959/1‑150 (MQ=255)
                                                                                           aCTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTT‑GA‑GAAACCACGCTCCAGCAGCGGTCAGCTCCACATCACGGTGCCGTCGTTCAAag                                                            <  1:1786013/150‑1 (MQ=255)
                                                                                            cTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGGTTTTCATTTTTTTGACAACAGAGCGCCCTTCTTT‑GT‑GAAAACCCCCTCCCGCCGCGGTCAGCTCCCCATAACGGTGGCGTCGTTCCAaga                                                           >  2:2156140/1‑150 (MQ=255)
                                                                                             tGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTT‑GA‑GAAACCACGCTCCAGCAGCGGTCAGCTCCACATCACGGTGCCGTCGTTCAAagag                                                          <  1:632366/150‑1 (MQ=255)
                                                                                              gCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTT‑GA‑GAAACCACGCTCCAGCAGCGGTCAGCTCCACATCACGGTGCCGTCGTTCAAAGAGc                                                         <  1:1274318/150‑1 (MQ=255)
                                                                                              gCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTT‑GA‑GAAACCACGCTCCAGCAGCGGTCAGCTCCACATCACGGGGCCGTCGTTCAAAGAGc                                                         >  1:4342811/1‑150 (MQ=255)
                                                                                               cGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTT‑GA‑GAAACCACGCTCCAGCAGCGGTCAGCTCCACATCACGTTGCCGTCGTTCAAAGAGCg                                                        <  2:3155679/150‑1 (MQ=255)
                                                                                               cGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTT‑GA‑GAAACCACGCTCCAGCAGCGGTCAGCTCCACATCACGGTGCCGTCGTTCAAAGAGCg                                                        >  1:3275155/1‑150 (MQ=255)
                                                                                               cGAGAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTT‑GA‑GAAACCACGCTCCAGCAGCGGTCAGCTCCACATCACGGTGCCGTCGTTCAAAGAGCg                                                        <  2:3005546/150‑1 (MQ=255)
                                                                                                gATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTT‑GA‑GAAACCACGCTCCAGCAGCGGTCAGCTCCACATCACGGTGCCGTCGTTCAAAGAGCgg                                                       <  2:358/150‑1 (MQ=255)
                                                                                                 aTAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATTTGCATTTTTTTGGCAACAGAGCGCCCTTCTTT‑GG‑GAAACCACGCCCCAGCAGCGGTCAGCCCCCCCTCACCGTGCCCTCGTTCAAAGAggggg                                                      >  2:3819449/1‑149 (MQ=255)
                                                                                                 aTAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTTACAAAAGAACGCCCCTCTTT‑TA‑GAAACCACGCTCCAGCACCCGGCAACTCCCCATCCCAGGGCCGTCGTTCAAACAGCGGc                                                      >  2:1200584/1‑150 (MQ=255)
                                                                                                   aGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTT‑GA‑GAAACCACGCTCCAGCAGCGGTCAGCTCCACATCACGGTGCCGTCGTTCAAAGAGCGGCAc                                                    <  1:3072587/150‑1 (MQ=255)
                                                                                                   aGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGATCTGCATTTTTTTTACAACAGAGCGCCCTTCTTT‑GA‑GAAACCACGCTCCAGCAGCGGTCAGCTCCACATCACGGGGCCGTCGTTCAAAGAGCGGccc                                                    >  2:1652108/1‑148 (MQ=255)
                                                                                                    gCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTT‑GA‑GAAACCACGCTCCAGCAGCGGTCAGCTCCACATCACCGTGCCGTCGTTCAAAGAGCGGCAcc                                                   >  1:3542819/1‑150 (MQ=255)
                                                                                                     cTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTT‑GA‑GAAACCACGCTCCAGCAGCGGTCAGCTCCACATCACGGTGCCGTCGTTCAAAGAGCGGCACCg                                                  >  1:4495057/1‑150 (MQ=255)
                                                                                                      tAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTT‑GA‑GAAACCACGCTCCAGCAGCGGTCAGCTCCACATCACGGTGCCGTCGTTCAAAGAGCGGCACCgc                                                 <  2:213074/150‑1 (MQ=255)
                                                                                                      tAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGATCTGCCTTTTTTTTACAAAAGAGCGCCCCTCTTT‑TA‑GAAACCACCCCCCCGCAGCGGGCAGCTCCACATTACGGTGGCGGCGTTTCAAGAGGGGGCCCgc                                                 >  2:4627246/1‑150 (MQ=255)
                                                                                                       aaCTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTT‑GA‑GAAACCACGCTCCAGCAGCGGTCAGCTCCACATCACCGTGCCGTCGTTCAAAGAGCGGCACCgcc                                                >  1:4293743/1‑150 (MQ=255)
                                                                                                                   cAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGATCTGCATTTTTTTTACAACAAAGCGCCCCTCTTT‑GA‑GAAACCACGCTCCAGCAGCGGGCAGCTCCCCATCACCGGGCCGTCGGTCAAAGAGCGGCCCCCCCCGACACTCtttc                                    >  2:2270166/1‑148 (MQ=255)
                                                                                                                         ttCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTTACAACAGAGCGCCCTTCTTT‑GA‑GAAACCACGCTCCAGCAGCGGTCAGCTCCACATCACCGTGCCGTCGTTCAAAGAGCGGCACCGCCAGCCACTCTTCCAGCTGa                              >  1:2458333/1‑150 (MQ=255)
                                                                                                                         ttCGCTATCTGCTGACATTGCTGGCGGGGGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTT‑GA‑GAAACCACGCTCCAGCAGCGGTCAGCTCCACATCACGGTGCCGTCGTTCAAAGAGCGGCACCCCCAGCCACTCTTCCCGCTGa                              >  2:1589398/1‑150 (MQ=255)
                                                                                                                               aTCTGCTGACATTGCTGGCGGGTGATCTGCCTTTTTTTTACAACAGAGCGCCCTTCTTT‑GA‑GAAACCACGCCCCAGCAGCGGGCAGCTCCACCTCACCGTGCCGGCGTTCCAAGAGCGGCCCCGCCCGCCACCCTTCCCGCTGACGCACg                        >  2:972528/1‑150 (MQ=255)
                                                                                                                                 ctgctgACATTGCTGGCGGGTGATCTGCATTTTTTTTACAACAGAGCGCCCTTCTTT‑TA‑GAAACCACGCTCCAGCAGCGGTCAGCTCCACATCACGGTGCCGTCGTTCAAAGAGCGGCACCGCCAGCCACTCTTCCAGCTGACGCACGGt                      >  1:3346580/1‑150 (MQ=255)
                                                                                                                                    ctgACATTGCTGGCGGGGGGTCTGCATTTTTTTTACAACAAAGCGCCCTTCTTT‑GA‑GAAACCACGCTCCAGCAGCGGGCAGCTCCACATCACGGTGCCGTCGTTCAAAGAGCGGCCCCGCCAGCCACTCTTCCAGCTGACGCACGGtata                   >  2:3294688/1‑150 (MQ=255)
                                                                                                                                       aCATTGCTGGCGGGGGATCTGCATTTTTTTTACAAAAAAGCGCCCTTCTTT‑GA‑GAAACCACGCTCCAGCAGCGGTCAGCTCCACATCCCGGTGCCGTCGTTCAAAGAGCGGCCCCGCCAGCCCCTCTTCCCGCTGACGCCCGGGATAGCt                >  2:2700042/1‑150 (MQ=255)
                                                                                                                                             cTGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTT‑GA‑GAAACCACGCTCCAGCAGCGGTCAGCTCCACATCACGGTGCCGTCGTTCAAAGAGCGGCACCGCCAGCCACTCTTCCAGCTGACGCACGGTATAGCTGACCGc          >  1:378363/1‑150 (MQ=255)
                                                                                                                                             cTGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTT‑GA‑GAAACCACGCTCCAGCAGCGGTCAGCTCCACATCACGGTGCCGTCGTTCAAAGAGCGGCACCGCCAGCCACTCTTCCAGCTGACGCACGGTATAGCTGACCGc          >  2:782503/1‑150 (MQ=255)
                                                                                                                                               ggCGGGTGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTT‑GA‑GAAACCACGCTCCAGCAGCGGTCAGCTCCACATCACGGTGCCGTCGTTCAAAGAGCGGCACCGCCAGCCACTCTTCCAGCTGACGCACGGTATAGCTGACCGCAg        >  2:4174887/1‑150 (MQ=255)
                                                                                                                                                    gTGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTT‑GA‑GAAACCACGCTCCAGCAGCGGTCAGCTCCACATCACGGTGCCGTCGTTCAAAGAGCGGCACCGCCAGCCACTCTTCCAGCTGACGCACGGTATAGCTGACCGCAGAAGGa   >  1:2158544/1‑150 (MQ=255)
                                                                                                                                                     tGATCTGCTTTTTTTAGAAAAAAG‑GCCCCCTTTTTTGGA‑AAAACCCCCCCCAAGCCGCGCCCAGCCCCACTCCTCTGGCCCGCCTTAAAAAGAGCGGCCCCCCCAGCCACTCTTCCAGCTGACGCACGGTATAGCTGACCGCAGAAGGaa  <  2:3200309/150‑1 (MQ=255)
                                                                                                                                                     tGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTT‑GA‑GAAACCACGCTCCAGCAGCGGTCAGCTCCACATCACGGTGCCGTCGTTCAAAGAGCGGCACCGCCAGCCACTCTTCCAGCTGACGCACGGTATAGCTGACCGCAGAAGGaa  >  2:730325/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                       
TTCCTGAAAGACAAGAAGTTCGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTT‑GA‑GAAACCACGCTCCAGCAGCGGTCAGCTCCACATCACGGTGCCGTCGTTCAAAGAGCGGCACCGCCAGCCACTCTTCCAGCTGACGCACGGTATAGCTGACCGCAGAAGGAA  >  AP012306/1420998‑1421296

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑