Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123062,857,9110AC23.3% 589.7 / 23.9 219K180N (AAA→AAC) aaeRpredicted DNA‑binding transcriptional regulator, efflux system
Reads supporting (aligned to +/- strand):  ref base A (141/27);  new base C (0/51);  total (141/78)
Fisher's exact test for biased strand distribution p-value = 2.53e-30
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 257 total aligned reads displayed.

GCCCCCGACCTGATTGCCGACGGTCTGGATGTGGTGATCCGCGTCGGCGCGTTGCAGGATTCCAGCC‑TGTTTTCCCGCCGTCTGGGCGCGATGCCAATGGTGGTGTGCGCCGCGAAAAGCTATCTCACACAATACGGCATACCGGAAAAA‑CCCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCTGATCCCACAAGGAAGATTTGTGACTAATGATCCGATGACGCTGGTGCGCTGGCT  >  AP012306/2857762‑2858060
                                                                                                                                                      |                                                                                                                                                      
gCCCCCGACCAGATGGCCGACGGTCTGGATGTGGTGATCCGCGTCGGCGCGTTGCAGGATTCCAGCC‑TGTTTTCCCGCCGTCTGGGCGCGATGCCAATGGTGGTGTGCGCCGCGAAAAGCTATCTCACACAATACGGCATACCGGaaaaa                                                                                                                                                        <  1:2250774/150‑1 (MQ=255)
  ccccGACCTGATTGCCGACGGTCTGGATGTGGTGATCCGCGTCGGCGCGTTGCAGGATTCCAGCC‑TGTTTTCCCGCCGTCTGGGCGCGATGCCAATGGTGGTGTGCGCCGCGAAAAGCTATCTCACACAATACGGCATACCGGAAAAA‑cc                                                                                                                                                     <  2:4506553/150‑1 (MQ=255)
  ccccGACCTGATTGCCGACGGTCTGGATGTGGTGATCCGCGTCGGCGCGTTGCAGGATTCCAGCC‑TGTTTTCCCGCCGTCTGGGCGCGATGCCAATGGTGGTGTGCGCCGCGAAAAGCTATCTCACACAATACGGCATACCGGAAAAA‑cc                                                                                                                                                     <  1:2714169/150‑1 (MQ=255)
   cccGACCTGATTGCCGACGGTCTGGATGTGGTGATCCGCGTCGGCGCGTTGCAGGATTCCAGCC‑TGTTTTCCCGCCGTCTGGGCGCGATGCCAATGGTGGTGTGCGCCGCGAAAAGCTATCTCACACAATACGGCATACCGGAAAAA‑ccc                                                                                                                                                    >  1:2619354/1‑150 (MQ=255)
    ccGACCTGATTGCCGACGGTCTGGATGTGGTGATCCGCGTCGGCGCGTTGCAGGATTCCAGCC‑TGTTTTCCCGCCGTCTGGGCGCGATGCCAATGGTGGTGTGCGCCGCGAAAAGCTATCTCACACAATACGGCATACCGGAAAAA‑Cccg                                                                                                                                                   <  1:4387456/150‑1 (MQ=255)
      gACCTGATTGCCGACGGTCTGGATGTGGTGATCCGCGTCGGCGCGTTGCAGGATTCCAGCC‑TGTTTTCCCGCCGTCTGGGCGCGATGCCAATGGTGGTGTGCGCCGCGAAAAGCTATCTCACACAATACGGCATACCGGAAAAA‑Cccgcc                                                                                                                                                 <  2:2374094/150‑1 (MQ=255)
         cTGATTGCCGACGGTCTGGATGTGGTGATCCGCGTCGGCGCGTTGCAGGATTCCAGCC‑TGTTTTCCCGCCGTCTGGGCGCGATGCCAATGGTGGTGTGCGCCGCGAAAAGCTATCTCACACAATACGGCATACCGGAAAAA‑CCCGCCGAt                                                                                                                                              >  1:2838409/1‑150 (MQ=255)
              tGCCGACGGTCTGGATGTGGTGATCCGCGTCGGCGCGTTGCAGGATTCCAGCC‑TGTTTTCCCGCCGTCTGGGCGCGATGCCAATGGTGGGGTGCGCCGCGAAAAGCTATCTCACACAATACGGCATACCGGAAAAA‑CCCGCCGATTTGag                                                                                                                                         >  2:4018239/1‑150 (MQ=255)
              cccggccGGTGTGGATGTGGTGTCCCGCGCGGGCGCGTGGCAGAATCCCAGCT‑TTTTTCCCCGCCGTCGGGGCGCGATGCCAATGGTGGTGTGCGCCGCAAAAGGCTACCCCCCACAATACGGCATCCCGAAAAAC‑CCCGCCGATTTGag                                                                                                                                         <  2:939488/144‑1 (MQ=255)
                ccGACGGTGTGGATGTGGTTATCCGCGTCGGCGCGTTGAAGCTTCCCAGCT‑TTTTTCCCCCCCGTCTGGGCGCGATGCCAATGGAGGTGTGCGCGGCAAAAATCTATCCCACAAAATACGGCATCCCGAAAAAA‑CCCGCCGATTTGagta                                                                                                                                       <  2:2571821/150‑1 (MQ=255)
                  gACGGTCTGGATGTGGTGATCCGCGTCGGCGCGTTGCAGGATTCCAGCC‑TGTTTTCCCGCCGTCTGGGCGCGATGCCAATGGTGGTGAGCGCCGCGAAAAGCTATCTCACACAATACGGCATACCGGAAAAA‑CCCGCCGATTTGagtagt                                                                                                                                     >  1:4344606/1‑150 (MQ=255)
                        cTGGATGTGGTGATCCGCGTCGGCGCGTTGCAGGATTCCAGCC‑TGTTTTCCCGCCGTCTGGGCGCGATGCCAATGGTGGTGTGCGCCGCGAAAAGCTATCTCACACAATACGGCATACCGGAAAAA‑CCCGCCGATTTGAGTAGtcattca                                                                                                                               >  2:2427883/1‑150 (MQ=255)
                             tgtgGTGATCCGCGTCGGCGCGTTGCAGGATTCCAGCC‑TGTTTTCCCGCCGTCTGGGCGCGATGCCAATGGTGGTGTGCGCCGCGAAAAGCTATCTCACACAATACGGCATACCGGAAAAA‑CCCGCCGATTTGAGTAGTCATTCATGGCt                                                                                                                          >  1:507564/1‑150 (MQ=255)
                             tgtgGTGATCCGCGTCGGCGCGTTGCAGGATTCCAGCC‑TGTTTTCCCGCCGTCTGGGCGCGATGCCAATGGTGGTGTGCGCCGCGAAAAGCTATCTCACACAATACGGCATACCGGAAAAA‑CCCGCCGATTTGAGTAGTCATTCATGGCt                                                                                                                          >  1:3227582/1‑150 (MQ=255)
                              gtggtgATCCGCGTCGGCGCGTTGCAGGATTCCAGCC‑TGTTTTCCCGCCGTCTGGGCGCGATGCCAATGGTGGTGTGCGCCGCGAAAAGCTATCTCACACAATACGGCATACCGGAAAAA‑CCCGCCGATTTGAGTAGTCATTCATTGCtt                                                                                                                         >  1:1022107/1‑150 (MQ=255)
                                   gATCCGCGTCGGCGCGTTGCAGGATTCCAGCC‑TGTTTTCCCGCCGTCTGGGCGCGATGCCAATGGTGGTGTGCGCCGCGAAAAGCTATCTCACACAATACGGCATACCGGAAAAA‑CCCGCCGATTTGAGTAGTCATTCATGGCTTGAATa                                                                                                                    >  2:3371883/1‑150 (MQ=255)
                                    aTCCGCGTCGGCGCGTTGCAGGATTCCAGCC‑TGTTTTCCCGCCGTCTGGGCGCGATGCCAATGGTGGTGTGCGCCGCGAAAAGCTATCTCACACAATACGGCATACCGGAAAAA‑CCCGCCGATTTGAGTAGTCATTCATGGCTTGAATAc                                                                                                                   >  2:2306788/1‑150 (MQ=255)
                                     tCCGCGGGGGCGTGTGGCAGTATCCCAGCC‑TTTTTCCCCGCCGTCGGGGCGCGATGCCAATGGGGGTGTGCGCCGCGAAAAGCTATCTCACACAATACGGCATACCGAAAAAC‑CCCGCCGATTTGAGTAGTCATTCATGGCTTGAATACa                                                                                                                  <  2:602765/150‑1 (MQ=255)
                                      ccGCGTGGGCGTGTGGAGGGATCCCAGCC‑TGTTTCCCCGCCGTCGGGGCGCGATGCCAGGGGTGGTGGGCGCCGCAAAAAGCTATCCCACAAAATACGGCATACCGAAAAAC‑CCCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAg                                                                                                                 <  2:4210230/150‑1 (MQ=255)
                                                  gTTGCAGGATTCCAGCC‑TGTTTTCCCGCCGTCTGGGCGCGATGCCAATGGTGGTGTGCGCCGCGAAAAGCTATCTCACACAATACGGCATACCGGAAAAA‑CCCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGa                                                                                                     >  2:1346642/1‑150 (MQ=255)
                                                    ttgatggttCCCGCCTTGTTTCCCCCCCGTCGGGGCGTGATGCAAAGGGTGGTGGGCGCCGAGAAAAGCTATCTCACACAATACGGCATCCCGAAAAAC‑CCCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGAc                                                                                                    <  2:2636780/143‑1 (MQ=255)
                                                      cAGGATTCCAGCC‑TGTTTTCCCGCCGTCTGGGCGCGATGCCAATGGTGGTGTGCGCCGCGAAAAGCTATCTCACACAATACGGCATACCGGAAAAA‑CCCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAAt                                                                                                 >  1:2110620/1‑150 (MQ=255)
                                                       aGGATTCCAGCC‑TGTTTTCCCGCCGTCTGGGCGCGATGCCAATGGTGGTGTGCGCCGCGAAAAGCTATCTCACACAATACGGCATACCGGAAAAA‑CCCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATg                                                                                                >  1:4428209/1‑150 (MQ=255)
                                                        ggATTCCAGCC‑TGTTTTCCCGCCGTCTGGGCGCGATGCCAATGGTGGTGTGCGCCGCGAAAAGCTATCTCACACAATACGGCATACCGGAAAAA‑CCCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGa                                                                                               >  2:3791743/1‑150 (MQ=255)
                                                             ccAGCC‑TGTTTTCCCGCCGTCTGGGCGCGATGCCAATGGTGGTGTGCGCCGCGAAAAGCTATCTCACACAATACGGCATACCGGAAAAA‑CCCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTg                                                                                          >  1:1532745/1‑150 (MQ=255)
                                                                gCC‑TGTTTTCCCGCCGTCTGGGCGCGATGCCAATGGTGGTGTGCGCCGCGAAAAGCTATCTCACACAATACGGCATACCGGAAAAA‑CCCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAAc                                                                                       >  2:2683347/1‑150 (MQ=255)
                                                                 cc‑TTTTTCCCCCCCGTCGGGGCGCGATGCCAATGGTGGTGTGCGCCGCAAAAAGTTATCACACAAAATACGGAATACCGAAAAAC‑CCCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACt                                                                                      <  2:820539/150‑1 (MQ=255)
                                                                  cttttttcCCCGCCGTGGGGGCGCGATGCAAATGGGGGTGGGCGCCGCAAAAAGCTATCCAAAACAATCCGCCATACCGAAAAAC‑CCCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACt                                                                                      <  2:209681/146‑1 (MQ=255)
                                                                     ttttcCCCGCCGTCTGGGCGCGATGCAAATGGGGGTGGGCGCCGCAAAAAGCTATCCCACACAATACGGCATACGGAAAAAA‑CCCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGAt                                                                                   <  2:1442814/149‑1 (MQ=255)
                                                                     gTTTTCCCGCCGTCTGGGCGCGATGCCAATGGTGGTGTGCGCCGCGAAAAGCTATCTCACACAATACGGCATACCGGAAAAA‑CCCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGAt                                                                                   >  1:1568626/1‑150 (MQ=255)
                                                                     gTTTTCCCGCCGTCTGGGCGCGATGCCAATGGTGGTGTGCGCCGCGAAAAGCTATCTCACACAATACGGCATACCGGAAAAA‑CCCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGAt                                                                                   >  2:3660966/1‑150 (MQ=255)
                                                                             gccgTCTGGGCGCGATGCCAATGGTGGTGTGCGCCGCGAAAAGCTATCTCACACAATACGGCATACCGGAAAAA‑CCCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCgg                                                                           >  2:3754136/1‑150 (MQ=255)
                                                                             gccgTCTGGGCGCGATGCCAATGGTGGTGTGCGCCGCGAAAAGCTATCTCACACAATACAGCATACCGGAAAAA‑CCCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCgg                                                                           >  1:4040127/1‑150 (MQ=255)
                                                                                 tCTGGGCGCGATGCCAATGGTGGTGTGCGCCGCGAAAAGCTATCTCACACAATACGGCATACCGGAAAAA‑CCCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAgg                                                                       >  1:2056404/1‑150 (MQ=255)
                                                                                      gcggGACGCAAAGGGGGGTGTGCGCCGAAAAAATCTATCCCACAAAAT‑CGGGAAACCCGAAAAACCCCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGAtct                                                                  <  2:1849355/150‑1 (MQ=255)
                                                                                       cgcgATGCAATTGGGGGTGTGCCCCGCAAAAAGCTATCTCACACAATACGGCATACCGAAAAAC‑CCCGCAGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGAtctc                                                                 <  2:3941781/150‑1 (MQ=255)
                                                                                        gcgATGCCAATGGTGGTGTGCGCCGCGAAAAGCTATCTCACACAATACGGCATACCGGAAAAA‑CCCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCg                                                                >  2:1790898/1‑150 (MQ=255)
                                                                                           aTGCCAATGGTGGTGTGCGCCGCGAAAAGTTATCTCACACAATCCGGCATCCCGGAAAAC‑CCCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACt                                                             <  1:3646692/150‑1 (MQ=255)
                                                                                           aTGCCAATGGTGGTGTGCGCCGCGAAAAGCTATCTCACACAATACGGCATACCGGAAAAA‑CCCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACt                                                             >  2:2828486/1‑150 (MQ=255)
                                                                                             gcaaggggtggtGGGCGCCGCGAAAAGCTATCCCACAAAATAGGGCATCGCGGAAAAA‑CCCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCg                                                           <  2:372609/144‑1 (MQ=255)
                                                                                              ccAATGGTGGTGTGCGCCGCGAAAAGCTATCTCACACAATACGGCATACCGGAAAAA‑CCCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGc                                                          >  1:3413759/1‑150 (MQ=255)
                                                                                                atgggtggtGTGAGCCGGAAAAAGCTTTTACCAAAAAGAGGGAATACCGGAAAAA‑CCCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCt                                                        <  2:47251/147‑1 (MQ=255)
                                                                                                   ggtggtGGGCGCGGCAAAAAGCTACCTCACACAATACGGCAT‑CCGGAAAAACCCCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCTGAt                                                     <  1:2004838/150‑1 (MQ=255)
                                                                                                    gtggtgTGCGCCGCGAAAAGCTATCTCACACAATACGGCATACCGGAAAAA‑CCCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCTGATc                                                    >  2:3456767/1‑150 (MQ=255)
                                                                                                      ggtgTGCGCCGCGAAAAGCTATCTCACACAATACGGCATACCGGAAAAA‑CCCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCTGATccc                                                  >  2:4375762/1‑150 (MQ=255)
                                                                                                      ggtgTGCGCCGCGAAAAGCTATCTCACACAATACGGCATACCGGAAAAA‑CCCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCTGATccc                                                  >  1:1804510/1‑150 (MQ=255)
                                                                                                      ggtgTGCGCCGCGAAAAGCTATCTCACACAATACGGCATACCGGAAAAA‑CCCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCTGATccc                                                  >  2:2997209/1‑150 (MQ=255)
                                                                                                       gggcgcgcCGCAAAAAGCTATCTCACAAAATACGGCATACCGAAAAAC‑CCCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCTGATCCca                                                 <  2:3246877/146‑1 (MQ=255)
                                                                                                        tgtgCGCCGCGAAAAGCTATCTCACACAATACGGCATACCGGAAAAA‑CCCGCCGATTTGAGTAGTCATTCATGGCTTGAAAACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCTGATCCcac                                                >  2:108206/1‑150 (MQ=255)
                                                                                                         gggcgcCGCAAAAAGCTACCCAACACAATACGGCATACCGAAAAAC‑CCCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCTGATCCcaca                                               <  1:3726192/148‑1 (MQ=255)
                                                                                                          tgCGCCGCGAAAAGCTATCTCACACAATACGGCATACCGGAAAAA‑CCCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCTGATCCCACaa                                              >  1:4451844/1‑150 (MQ=255)
                                                                                                          tgCGCCGCAAAAATCAATCCCCCAAAATCCGCCACCCGGAAAAAA‑CCCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCTGATCCCACaa                                              <  2:697347/150‑1 (MQ=255)
                                                                                                            ccccgcAAAAAGTTACCCCAAAAAATGCGCCAT‑CCGGAAAAACCCCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCTGATCCCACAAgg                                            <  2:3071202/148‑1 (MQ=255)
                                                                                                             cccgaaaaaaGCTATCTCACAAAAACCGCAATCCCGAAAAAC‑CCCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCTGATCCCACAAGGa                                           <  2:3878308/144‑1 (MQ=255)
                                                                                                              ccgcGAAAAGCTATCTCACACAATACGGCATACCGGAAAAA‑CCCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCTGATCCCACAAGGaa                                          >  2:2497623/1‑150 (MQ=255)
                                                                                                              ccgcGAAAAGCAATCCCACACAATACGGCATACGGAAAAAT‑CCCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCTGATCCCACAAGGaa                                          <  2:2838805/150‑1 (MQ=255)
                                                                                                                gcaaaaaGCTATCACACACAATACGGCATACCGAAAAAC‑CCCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCTGATCCCACAAGGAAGa                                        <  1:3111597/147‑1 (MQ=255)
                                                                                                                 caaaaaGCTATCTCACAAAATACGGCATACCGAAAAAA‑CCCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCTGATCCCACAAGGAAGAt                                       <  1:1960751/148‑1 (MQ=255)
                                                                                                                 caaaaaGCTACCCCACACAATCCGGCATACCGAAAAAC‑CCCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCTGATCCCACAAGGAAGAt                                       <  2:1240184/148‑1 (MQ=255)
                                                                                                                    aaaGCTATCTCACACAATACGGCATACCGGAAAAA‑CCCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCTGATCCCACAAGGAAGATTtg                                    >  1:122384/1‑150 (MQ=255)
                                                                                                                    aaaGCTATCTCACACAATACGGCATACCGGAAAAA‑CCCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCTGATCCCACAAGGAAGATTtg                                    >  1:2325023/1‑150 (MQ=255)
                                                                                                                     aaGCTATCTCACACAATACGGCATACCGGAAAAA‑CCCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCTGATCCCACAAGGAAGATTtgt                                   >  2:1001907/1‑150 (MQ=255)
                                                                                                                        cTATCTCACACAATACGGCATACCGGAAAAA‑CCCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCTGATCCCACAAGGAAGATTTGTGAc                                >  1:655626/1‑150 (MQ=255)
                                                                                                                        cTATCTCACACAATACGGCATACCGGAAAAA‑CCCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGCGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCTGATCCCACAAGGAAGATTTGTGAc                                >  1:2771765/1‑150 (MQ=255)
                                                                                                                         tATCTCACACAATACGGCATACCGGAAAAA‑CCCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCTGATCCCACAAGGAAGATTTGTGACt                               >  1:1680072/1‑150 (MQ=255)
                                                                                                                           tctcACACAATACGGCATACCGGAAAAA‑CCCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCTGATCCCACAAGGAAGATTTGTGACTaa                             >  1:926134/1‑150 (MQ=255)
                                                                                                                            ctcACACAATACGGCATACCGGAAAAA‑CCCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCTGATCCCACAAGGAAGATTTGTGACTAAt                            >  1:1012277/1‑150 (MQ=255)
                                                                                                                              cacacaACACGCCTTCCCGGAAAAC‑CCCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCTGATCCCACAAGGAAGATTTGTGACTAATGa                          <  1:1625895/150‑1 (MQ=255)
                                                                                                                               acacaATACGGCATACCGGAAAAA‑CCCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCTGATCCCACAAGGAAGATTTGTGACTAATGAt                         >  1:1361902/1‑150 (MQ=255)
                                                                                                                               acacaATACGGCATACCGGAAAAA‑CCCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCTGATCCCACAAGGAAGATTTGTGACTAATGAt                         >  2:2817217/1‑150 (MQ=255)
                                                                                                                                caaaacaCGTCATGCCGAAAAAC‑CCCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCTGATCCCACAAGGAAGATTTGTGACTAATGATc                        <  2:833254/144‑1 (MQ=255)
                                                                                                                                aaaaaTAGGGCAT‑CCGGAAAAACCCCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCTGATCCCACAAGGAAGATTTGTGACTAATGATc                        <  2:2182107/147‑1 (MQ=255)
                                                                                                                                 acaATACGGCATACGGGAAAAA‑CCCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCTGATCCCACAAGGAAGATTTGTGACTAATGATcc                       <  1:306417/150‑1 (MQ=255)
                                                                                                                                 acaATACGGCAT‑CCGGAAAAACCCCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCTGATCCCACAAGGAAGATTTGTGACTAATGATcc                       <  1:3764401/150‑1 (MQ=255)
                                                                                                                                 acaAGACGGCATACCGAAAAAC‑CCCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCTGATCCCACAAGGAAGATTTGTGACTAATGATcc                       <  1:3706922/150‑1 (MQ=255)
                                                                                                                                  caATCCGCCATCCCGAAAAAC‑CCCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCTGATCCCACAAGGAAGATTTGTGACTAATGATCCg                      <  1:1287594/150‑1 (MQ=255)
                                                                                                                                  aaaTCCGGCAACCCGAAAAAC‑CCCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCTGATCCCACAAGGAAGATTTGTGACTAATGATCCg                      <  2:4193717/149‑1 (MQ=255)
                                                                                                                                   aaTACGGCATACCGGAAAAA‑CCCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCTGATCCCACAAGGAAGATTTGTGACTAATGATCCGa                     >  1:2537652/1‑150 (MQ=255)
                                                                                                                                    aTACGGCATACCGGAAAAA‑CCCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCTGATCCCACAAGGAAGATTTGTGACTAATGATcc                       >  1:3921632/1‑147 (MQ=255)
                                                                                                                                    aTACGGCATACCGGAAAAA‑CCCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCTGATCCCACAAGGAAGATTTGTGACTAATGATCCGAt                    >  1:1593067/1‑150 (MQ=255)
                                                                                                                                      gcGGCATACCGAAAAAC‑CCCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCTGATCCCACAAGGAAGATTTGTGACTAATGATCCGATGa                  <  1:2167586/149‑1 (MQ=255)
                                                                                                                                      aCGGCATACCGGAAAAA‑CCCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCTGATCCCACAAGGAAGATTTGTGACTAATGATCCGATGa                  >  2:1965769/1‑150 (MQ=255)
                                                                                                                                       cGGCATACCGGAAAAA‑CCCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCTGATCCCACAAGGAAGATTTGTGACTAATGATCCGATGAc                 >  1:4550969/1‑150 (MQ=255)
                                                                                                                                       cGGCATACCGGAAAAA‑CCCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCTGATCCCACAAGGAAGATTTGTGACTAATGATCCGATGAc                 >  1:2974914/1‑150 (MQ=255)
                                                                                                                                        ggCATCCGGGAAAAA‑CCCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCTGATCCCACAAGGAAGATTTGTGACTAATGATCCGATGACg                <  1:1455750/150‑1 (MQ=255)
                                                                                                                                        ggCATACCGGAAAAA‑CCCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCTGATCCCACAAGGAAGATTTGTGACTAATGATCCGATGaag                >  2:3190507/1‑148 (MQ=255)
                                                                                                                                        ggCATACCGAAAAAC‑CCCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCTGATCCCACAAGGAAGATTTGTGACTAATGATCCGATGACg                <  1:1631622/150‑1 (MQ=255)
                                                                                                                                           aTACCGGAAAAC‑CCCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCTGATCCCACAAGGAAGATTTGTGACTAATGATCCGATGACGCTg             <  1:148180/150‑1 (MQ=255)
                                                                                                                                           aTACCGAAAAAC‑CCCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCTGATCCCACAAGGAAGATTTGTGACTAATGATCCGATGACGCTg             <  1:1715788/150‑1 (MQ=255)
                                                                                                                                             acaaaaaaac‑CCCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCTGATCCCACAAGGAAGATTTGTGACTAATGATCCGATGACGCTGGt           <  2:2490418/145‑1 (MQ=255)
                                                                                                                                             aCCGGAAAAA‑CCCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCTGATCCCACAAGGAAGATTTGTGACTAATGATCCGATGACGCTGGt           >  1:2557/1‑150 (MQ=255)
                                                                                                                                              ccGGAAAAA‑CCCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCTGATCCCACAAGGAAGATTTGTGACTAATGATCCGATGACGCTGGTg          >  2:1770560/1‑150 (MQ=255)
                                                                                                                                              ccGGAAAAA‑CCCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCTGATCCCACAAGGAAGATTTGTGACTAATGATCCGATGACGCTGGTg          >  1:3588564/1‑150 (MQ=255)
                                                                                                                                                gaaaaac‑CCCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCTGATCCCACAAGGAAGATTTGTGACTAATGATCCGATGACGCTGGTgcg        <  2:3560993/148‑1 (MQ=255)
                                                                                                                                                 gAAAAA‑CCCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCTGATCCCACAAGGAAGATTTGTGACTAATGATCCGATGACGCTGGTgcgc       >  1:1213609/1‑150 (MQ=255)
                                                                                                                                                 gAAAAA‑CCCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCTGATCCCACAAGGAAGATTTGTGACTAATGATCCGATGACGCTGGTgcgc       >  2:1705664/1‑150 (MQ=255)
                                                                                                                                                  aaaaa‑CCCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCTGATCCCACAAGGAAGATTTGTGACTAATGATCCGATGACGCTGGTGCgct      >  2:2769391/1‑150 (MQ=255)
                                                                                                                                                   aaaa‑CCCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCTGATCCCACAAGGAAGATTTGTGACTAATGATCCGATGACGCTGGTGCgctg     >  1:3326700/1‑150 (MQ=255)
                                                                                                                                                     aa‑CCCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCTGATCCCACAAGGAAGATTTGTGACTAATGATCCGATGACGCTGGTGCgctggc   >  1:398137/1‑150 (MQ=255)
                                                                                                                                                      a‑CCCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCTGATCCCACAAGGAAGATTTGTGACTAATGATCCGATGACGCTGGTGCgctggct  <  1:3812337/150‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                      
GCCCCCGACCTGATTGCCGACGGTCTGGATGTGGTGATCCGCGTCGGCGCGTTGCAGGATTCCAGCC‑TGTTTTCCCGCCGTCTGGGCGCGATGCCAATGGTGGTGTGCGCCGCGAAAAGCTATCTCACACAATACGGCATACCGGAAAAA‑CCCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCTGATCCCACAAGGAAGATTTGTGACTAATGATCCGATGACGCTGGTGCGCTGGCT  >  AP012306/2857762‑2858060

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑