BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AP012306	3,407,932	C→T	S40L (TCA→TTA)	wzyE →	predicted Wzy protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AP012306	3,407,932	0	C	T	99.7%	1326.7 / ‑4.5	324	S40L (TCA→TTA)	wzyE	predicted Wzy protein
Reads supporting (aligned to +/- strand): ref base C (0/1); new base T (163/160); total (163/161)
Fisher's exact test for biased strand distribution p-value = 4.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.20e-01

Only 100 of 329 total aligned reads displayed.

GCGTTGGCGATTGCCGCCAGGGAGGTCGCATGAGTCTGCTGCAATTCAGTGGCCTGTTTGTTGTCTGGCTGCTCTGCACGCTGTTTATTGCCACGCTGACCTGGTTTGAGTTTCGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTCATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCGCTGACCAGCGTGCTGGTATTTCGCTTTGATGTTGGTGTCGCGCCGCCAGAAATCTTGTTGCAGGCGTTGCTTTCTGCGGGCTGCTTCTACGCGGTTTACTATGTCACCT  >  AP012306/3407785‑3408078

   ttGGCGATTGCCGCCAGGGAGGTCGCATGAGTCTGCTGCAATTCAGTGGCCTGTTTGTTGTCTGGCTGCTCTGCACGCTGTTTATTGCCACGCTGACCTGGTTTGAGTTTCGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTTATTGc                                                                                                                                               <  1:1906671/150‑1 (MQ=255)

    tGGCGATTGCCGCCAGGGAGGTCGCATGAGTCTGCTGCAATTCAGTGGCCTGTTTGTTGTCTGGCTGCTCTGCACGCTGTTTATTGCCACGCTGACCTGGTTTGAGTTTCGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTTATTGCt                                                                                                                                              <  1:3110873/150‑1 (MQ=255)

       cGATTGCCGCCAGGGAGGTCGCATGAGTCTGCTGCAATTCAGTGGCCTGTTTGTTGTCTGGCTGCTCTGCACGCTGTTTATTGCCACGCTGACCTGGTTTGAGTTTCGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGtt                                                                                                                                           >  2:4573520/1‑150 (MQ=255)

        gATTGCCGCCAGGGAGGTCGCATGAGTCTGCTGCAATTCAGTGGCCTGTTTGTTGTCTGGCTGCTCTGCACGCTGTTTATTGCCACGCTGACCTGGTTTGAGTTTCGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGttt                                                                                                                                          >  1:2497162/1‑150 (MQ=255)

        gATTGCCGCCAGGGAGGTCGCATGAGTCTGCTGCAATTCAGTGGCCTGTTTGTTGTCTGGCTGCTCTGCACGCTGTTTATTGCCACGCTGACCTGGTTTGAGTTTCGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGttt                                                                                                                                          >  2:3078807/1‑150 (MQ=255)

         aTTGCCGCCAGGGAGGTCGCATGAGTCTGCTGCAATTCAGTGGCCTGTTTGTTATCTGGCTGCTCTGCACGCTGTTTATTGCCACGCTGACCTGGTTTGAGTTTCGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGtttt                                                                                                                                         >  1:1945629/1‑150 (MQ=255)

           tGCCGCCAGGGAGGTCGCATGAGTCTGCTGCAATTCAGTGGCCTGTTTGTTGTCTGGCTGCTCTGCACGCTGTTTATTGCCACGCTGACCTGGTTTGAGTTTCGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGTTTTTg                                                                                                                                       <  1:3323197/150‑1 (MQ=255)

           tGCCGCCAGGGAGGTCGCATGAGTCTGCTGCAATTCAGTGGCCTGTTTGTTGTCTGGCTGCTCTGCACGCTGTTTATTGCCACGCTGACCTGGTTTGAGTTTCGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGTTTTTg                                                                                                                                       <  2:2941457/150‑1 (MQ=255)

             ccgccAGGGAGGTCGCATGAGTCTGCTGCAATTCAGTGGCCTGTTTGTTGTCTGGCTGCTCTGCACGCTGTTTATTGCCACGCTGACCTGGTTTGAGTTTCGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGTTTTTGCt                                                                                                                                     <  2:3123247/150‑1 (MQ=255)

             ccgccAGGGAGGTCGCATGAGTCTGCTGCAATTCAGTGGCCTGTTTGTTGTCTGGCTGCTCTGCACGCTGTTTATTGCCACGCTGACCTGGTTTGAGTTTCGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGTTTTTGCt                                                                                                                                     >  1:3448518/1‑150 (MQ=255)

               gccAGGGAGGTCGCATGAGTCTGCTGCAATTCAGTGGCCTGTTTGTTGTCTGGCTGCTCTGCACGCTGTTTATTGCCACGCTGACCTGGTTTGAGTTTCGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCa                                                                                                                                   >  2:1763738/1‑150 (MQ=255)

                ccAGGGAGGTCGCATGAGTCTGCTGCAATTCAGTGGCCTGTTTGTTGTCTGGCTGCTCTGCACGCTGTTTATTGCCACGCTGACCTGGTTTGAGTTTCGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCAc                                                                                                                                  <  2:3780773/150‑1 (MQ=255)

                ccAGGGAGGTCGCATGAGTCTGCTGCAATTCAGTGGCCTGTTTGTTGTCTGGCTGCTCTGCACGCTGTTTATTGCCACGCTGACCTGGTTTGAGTTTCGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCAc                                                                                                                                  <  2:4198127/150‑1 (MQ=255)

                    ggAGGTCGCATGAGTCTGCTGCAATTCAGTGGCCTGTTTGTTGTCTGGCTGCTCTGCACGCTGTTTATTGCCACGCTGACCTGGTTTGAGTTTCGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCttt                                                                                                                              >  2:4038628/1‑150 (MQ=255)

                     gAGGTCGCATGAGTCTGCTGCAATTCAGTGGCCTGTTTGTTGTCTGGCTGCTCTGCACGCTGTTTATTGCCACGCTGACCTGGTTTGAGTTTCGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCtttt                                                                                                                             <  1:340345/150‑1 (MQ=255)

                      aGGTCGCATGAGTCTGCTGCAATTCAGTGGCCTGTTTGTTGTCTGGCTGCTCTGCACGCTGTTTATTGCCACGCTGACCTGGTTTGAGTTTCGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCttttt                                                                                                                            <  1:660737/150‑1 (MQ=255)

                          cGCATGAGTCTGCTGCAATTCAGTGGCCTGTTTGTTGTCTGGCTGCTCTGCACGCTGTTTATTGCCACGCTGACCTGGTTTGAGTTTCGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTttcttc                                                                                                                        >  2:2962367/1‑150 (MQ=255)

                          cGCATGAGTCTGCTGCAATTCAGTGGCCTGTTTGTTGTCTGGCTGCTCTGCACGCTGTTTATTGCCACGCTGACCTGGTTTGAGTTTCGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTttcttc                                                                                                                        >  1:1227513/1‑150 (MQ=255)

                            cATGAGTCTGCTGCAATTCAGTGGCCTGTTTGTTGTCTGGCTGCTCTGCACGCTGTTTATTGCCACGCTGACCTGGTTTGAGTTTCGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCgg                                                                                                                      >  2:16796/1‑150 (MQ=255)

                            cATGAGTCTGCTGCAATTCAGTGGCCTGTTTGTTGTCTGGCTGCTCTGCACGCTGCTTATTGCGACGCTGACCTGGTTTGAGTTTCGCCGTGTGCGCTTTAACCTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCgg                                                                                                                      >  2:1911172/1‑150 (MQ=255)

                              tGAGTCTGCTGCAATTCAGTGGCCTGTTTGTTGTCTGGCTGCTCTGCACGCTGTTTATTGCCACGCTGACCTGGTTTGAGTTTCGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCt                                                                                                                    >  1:3876674/1‑150 (MQ=255)

                              tGAGTCTGCTGCAATTCAGTGGCCTGTTTGTTGTCTGGCTGCTCTGCACGCTGTTTATTGCCACGCTGACCTGGTTTGAGTTTCGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCt                                                                                                                    <  1:4627877/150‑1 (MQ=255)

                                  tCTGCTGCAATTCAGTGGCCTGTTTGTTGTCTGGCTGCTCTGCACGCTGTTTATTGCCACGCTGACCTGGTTTGAGTTTCGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTccc                                                                                                                <  1:1699513/150‑1 (MQ=255)

                                  tCTGCTGCAATTCAGTGGCCTGGTTGTTGTCTGGCTGCTCTGCACGCTTTTTATTGCCACGCTAACCTGGTTTGAGTTTCGCCGTGTGGGCCTTAACTTTTATGTCTTCTTGTTATTGCTGTTTTTGCTCACCTTTTTCTTCTGCTTccc                                                                                                                >  2:2492254/1‑150 (MQ=255)

                                   ctgctgCAATTCAGTGGCCTGTTTGTTGTCTGGCTGCTCTGCACGCTGTTTATTGCCACGCTGACCTGGTTTGAGTTTCGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCg                                                                                                               <  2:1352604/150‑1 (MQ=255)

                                     gctgcAATTCAGTGGCCTGTTTGTTGTCTGGCTGCTCTGCACGCTGTTTATTGCCACGCTGACCTGGTTTGAGTTTCGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCGCt                                                                                                             <  1:2953194/150‑1 (MQ=255)

                                      ctgcAATTCAGTGGCCTGTTTGTTGTCTGGCTGCTCTGCACGCTGTTTATTGCCACGCTGACCTGGTTTGAGTTTCGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCGCTg                                                                                                            >  1:2694153/1‑150 (MQ=255)

                                      ctgcAATTCAGTGGCCTGTTTGTTGTCTGGCTGCTCTGCACGCTGTTTATTGCCACGCTGACCTGGTTTGAGTTTCGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCGCTg                                                                                                            >  1:2681580/1‑150 (MQ=255)

                                         cAATTCAGTGGCCTGTTTGTTGTCTGGCTGCTCTGCACGCTGTTTATTGCCACGCTGACCTGGTTTGAGTTTCGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCGCTGAcc                                                                                                         <  2:125920/150‑1 (MQ=255)

                                          aaTTCAGTGGCCTGTTTGTTGTCTGGCTGCTCTGCACGCTGTTTATTGCCACGCTGACCTGGTTTGAGTTTCGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCGCTGACCa                                                                                                        >  2:548251/1‑150 (MQ=255)

                                           aTTCAGTGGCCTGTTTGTTGTCTGGCTGCTCTGCACGCTGTTTATTGCCACGCTGACCTGGTTTGAGTTTCGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCGCTGACCAg                                                                                                       <  1:2378996/150‑1 (MQ=255)

                                                 tGGCCTGTTTGTTGTCTGGCTGCTCTGCACGCTGTTTATTGCCACGCTGACCTGGTTTGAGTTTCGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCGCTGACCAGCGTGCt                                                                                                 >  2:4067034/1‑150 (MQ=255)

                                                  ggCCTGTTTGTTGTCTGGCTGCTCTGCACGCTGTTTATTGCCACGCTGACCTGGTTTGAGTTTCGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCGCTGACCAGCGTGCTg                                                                                                <  1:2608140/150‑1 (MQ=255)

                                                  ggCCTGTTTGTTGTCTGGCTGCTCTGCACGCTGTTTATTGCCACGCTGACCTGGTTTGAGTTTCGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCGCTGACCAGCGTGCTg                                                                                                >  2:4455276/1‑150 (MQ=255)

                                                   gCCTGTTTGTTGTCTGGCTGCTCTGCACGCTGTTTATTGCCACGCTGACCTGGTTTGAGTTTCGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCGCTGACCAGCGTGCTgg                                                                                               <  2:4138855/150‑1 (MQ=255)

                                                    ccTGTTTGTTGTCTGGCTGCTCTGCACGCTGTTTATTGCCACGCTGACCTGGTTTGAGTTTCGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCGCTGACCAGCGTGCTGGt                                                                                              <  1:1333465/150‑1 (MQ=255)

                                                    ccTGTTTGTTGTCTGGCTGCTCTGCACGCTGTTTATTGCCACGCTGACCTGGTTTGAGTTTCGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCGCTGACCAGCGTGCTGGt                                                                                              >  2:2595243/1‑150 (MQ=255)

                                                     cTGTTTGTTGTCTGGCTGCTCTGCACGCTGTTTATTGCCACGCTGACCTGGTTTGAGTTTCGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCGCTGACCAGCGTGCTGGTa                                                                                             >  1:2261020/1‑150 (MQ=255)

                                                       gtttgttGTCTGGCTGCTCTGCACGCTGTTTATTGCCACGCTGACCTGGTTTGAGTTTCGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCGCTGACCAGCGTGCTGGTAtt                                                                                           >  2:3414520/1‑150 (MQ=255)

                                                       gtttgttGTCTGGCTGCTCTGCACGCTGTTTATTGCCACGCTGACCTGGTTTGAGTTTCGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCGCTGACCAGCGTGCTGGTAtt                                                                                           >  2:2394582/1‑150 (MQ=255)

                                                       gtttgttGTCTGGCTGCTCTGCACGCTGTTTATTGCCACGCTGACCTGGTTTGAGTTTCGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCGCTGACCAGCGTGCTGGTAtt                                                                                           <  2:2668312/150‑1 (MQ=255)

                                                       gtttgttGTCTGGCTGCTCTGCACGCTGTTTATTGCCACGCTGACCTGGTTTGAGTTTCGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCGCTGACCAGCGTGCTGGTAtt                                                                                           >  1:3335709/1‑150 (MQ=255)

                                                        tttgttGTCTGGCTGCTCTGCACGCTGTTTATTGCCACGCTGACCTGGTTTGAGTTTCGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCGCTGACCAGCGTGCTGGTAttt                                                                                          >  2:629350/1‑150 (MQ=255)

                                                           gttgtCTGGCTGCTCTGCACGCTGTTTATTGCCACGCTGACCTGGTTTGAGTTTCGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCGCTGACCAGCGTGCTGGTATTTCGc                                                                                       >  2:2219388/1‑150 (MQ=255)

                                                             tgtCTGGCTGCTCTGCACGCTGTTTATTGCCACGCTGACCTGGTTTGAGTTTCGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCGCTGACCAGCGTGCTGGTATTTCGCtt                                                                                     <  1:2807975/150‑1 (MQ=255)

                                                             tgtCTGGCTGCTCTGCACGCTGTTTATTGCCACGCTGACCTGGTTTGAGTTTCGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCGCTGACCAGCGTGCTGGTATTTCGCtt                                                                                     >  2:722301/1‑150 (MQ=255)

                                                                cTGGCTGCTCTGCACGCTGTTTATTGCCACGCTGACCTGGTTTGAGTTTCGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCGCTGACCAGCGTGCTGGTATTTCGCTTTGa                                                                                  >  1:4329117/1‑150 (MQ=255)

                                                                  ggCTGCTCTGCACGCTGTTTATTGCCACGCTGACCTGGTTTGAGTTTCGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCGCTGACCAGCGTGCTGGTATTTCGCTTTGATg                                                                                <  2:2330787/150‑1 (MQ=255)

                                                                   gctgctCTGCACGCTGTTTATTGCCACGCTGACCTGGTTTGAGTTTCGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCGCTGACCAGCGTGCTGGTATTTCGCTTTGATGt                                                                               <  2:195313/150‑1 (MQ=255)

                                                                    ctgctCTGCACGCTGTTTATTGCCACGCTGACCTGGTTTGAGTTTCGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCGCTGACCAGCGTGCTGGTATTTCGCTTTGATGtt                                                                              <  1:1602771/150‑1 (MQ=255)

                                                                    ctgctCTGCACGCTGTTTATTGCCACGCTGACCTGGTTTGAGTTTCGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCGCTGACCAGCGTGCTGGTATTTCGCTTTGATGtt                                                                              >  1:3619162/1‑150 (MQ=255)

                                                                     tgctCTGCACGCTGTTTATTGCCACGCTGACCTGGTTTGAGTTTCGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCGCTGACCAGCGTGCTGGTATTTCGCTTTGATGTTg                                                                             <  2:2884163/150‑1 (MQ=255)

                                                                         ctGCACGCTGTTTATTGCCACGCTGACCTGGTTTGAGTTTCGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCGCTGACCAGCGTGCTGGTATTTCGCTTTGATGTTGgtgt                                                                         >  1:1661534/1‑150 (MQ=255)

                                                                            cACGCTGTTTATTGCCACGCTGACCTGGTTTGAGTTTCGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCGCTGACCAGCGTGCTGGTATTTCGCTTTGATGTTGGTGTcgc                                                                      <  2:1020650/150‑1 (MQ=255)

                                                                             aCGCTGTTTATTGCCACGCTGACCTGGTTTGAGTTTCGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCGCTGACCAGCGTGCTGGTATTTCGCTTTGATGTTGGTGTcgcg                                                                     >  2:1485370/1‑150 (MQ=255)

                                                                                   tttATTGCCACGCTGACCTGGTTTGAGTTTCGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCGCTGACCAGCGTGCTGGTATTTCGCTTTGATGTTGGTGTCGCGCCGCCa                                                               >  1:4347347/1‑150 (MQ=255)

                                                                                      aTTGCCACGCTGACCTGGTTTGAGTTTCGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCGCTGACCAGCGTGCTGGTATTTCGCTTTGATGTTGGTGTCGCGCCGCCAGaa                                                            >  2:1000251/1‑150 (MQ=255)

                                                                                        tGCCACGCTGACCTGGTTTGAGTTTCGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCGCTGACCAGCGTGCTGGTATTTCGCTTTGATGTTGGTGTCGCGCCGCCAGAAAt                                                          <  1:2033870/150‑1 (MQ=255)

                                                                                            aCGCTGACCTGGTTTGAGTTTCGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCGCTGACCAGCGTGCTGGTATTTCGCTTTGATGTTGGTGTCGCGCCGCCAGAAATCttg                                                      >  1:1263553/1‑150 (MQ=255)

                                                                                              gCTGACCTGGTTTGAGTTTCGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCGCTGACCAGCGTGCTGGTATTTCGCTTTGATGTTGGTGTCGCGCCGCCAGAAATCttgtt                                                    >  1:3539022/1‑150 (MQ=255)

                                                                                                 gACCTGGTTTGAGTTTCGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCGCTGACCAGCGTGCTGGTATTTCGCTTTGATGTTGGTGTCGCGCCGCCAGAAATCTTGTTGCa                                                 <  2:1225733/150‑1 (MQ=255)

                                                                                                  aCCTGGTTTGAGTTTCGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCGCTGACCAGCGTGCTGGTATTTCGCTTTGATGTTGGTGTCGCGCCGCCAGAAATCTTGTTGCAg                                                >  2:2094615/1‑150 (MQ=255)

                                                                                                    cTGGTTTGAGTTTCGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCGCTGACCAGCGTGCTGGTATTTCGCTTTGATGTTGGTGTCGCGCCGCCAGAAATCTTGTTGCAGGc                                              <  2:2052135/150‑1 (MQ=255)

                                                                                                     tGGTTTGAGTTTCGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCGCTGACCAGCGTGCTGGTATTTCGCTTTGATGTTGGTGTCGCGCCGCCAGAAATCTTGTTGCAGGCg                                             >  2:3360669/1‑150 (MQ=255)

                                                                                                      ggTTTGAGTTTCGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCGCTGACCAGCGTGCTGGTATTTCGCTTTGATGTTGGTGTCGCGCCGCCAGAAATCTTGTTGCAGGCGt                                            <  1:1307822/150‑1 (MQ=255)

                                                                                                       gTTTGAGTTTCGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCGCTGACCAGCGTGCTGGTATTTCGCTTTGATGTTGGTGTCGCGCCGCCAGAAATCTTGTTGCAGGCGtt                                           <  1:3435312/150‑1 (MQ=255)

                                                                                                        tttGAGTTTCGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCGCTGACCAGCGTGCTGGTATTTCGCTTTGATGTTGGTGTCGCGCCGCCAGAAATCTTGTTGCAGGCGTTg                                          >  1:3178611/1‑150 (MQ=255)

                                                                                                         ttGAGTTTCGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCGCTGACCAGCGTGCTGGTATTTCGCTTTGATGTTGGTGTCGCGCCGCCAGAAATCTTGTTGCAGGCGTTGc                                         >  1:1995781/1‑150 (MQ=255)

                                                                                                            aGTTTCGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCGCTGACCAGCGTGCTGGTATTTCGCTTTGATGTTGGTGTCGCGCCGCCAGAAATCTTGTTGCAGGCGTTGCttt                                      >  2:3679328/1‑150 (MQ=255)

                                                                                                            aGTTTCGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCGCTGACCAGCGTGCTGGTATTTCGCTTTGATGTTGGTGTCGCGCCGCCAGAAATCTTGTTGCAGGCGTTGCttt                                      >  2:3499839/1‑150 (MQ=255)

                                                                                                             gTTTCGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCGCTGACCAGCGTGCTGGTATTTCGCTTTGATGTTGGTGTCGCGCCGCCAGAAATCTTGTTGCAGGCGTTGCTTTc                                     <  1:3811213/150‑1 (MQ=255)

                                                                                                              tttCGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCGCTGACCAGCGTGCTGGTATTTCGCTTTGATGTTGGTGTCGCGCCGCCAGAAATCTTGTTGCAGGCGTTGCTTTCt                                    >  1:1406565/1‑150 (MQ=255)

                                                                                                                 cGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCGCTGACCAGCGTGCTGGTATTTCGCTTTGATGTTGGTGTCGCGCCGCCAGAAATCTTGTTGCAGGCGTTGCTTTCTGCg                                 >  2:3841176/1‑150 (MQ=255)

                                                                                                                  gCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCGCTGACCAGCGTGCTGGTATTTCGCTTTGATGTTGGTGTCGCGCCGCCAGAAATCTTGTTGCAGGCGTTGCTTTCTGCgg                                <  1:4271000/150‑1 (MQ=255)

                                                                                                                   ccGTGTGCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCGCTGACCAGCGTGCTGGTATTTCGCTTTGATGTTGGTGTCGCGCCGCCAGAAATCTTGTTGCAGGCGTTGCTTTCTGCggg                               <  2:1104536/150‑1 (MQ=255)

                                                                                                                    cGTGTGCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCGCTGACCAGCGCGCTGGTATTTCGCTTTGATGTTGGTGTCGCGCCGCCAGAAATCTTGTTGCAGGCGTTGCTTTCTGCGGgc                              >  1:759367/1‑150 (MQ=255)

                                                                                                                     gtgtGCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCGCTGACCAGCGTGCTGGTATTTCGCTTTGATGTTGGTGTCGCGCCGCCAGAAATCTTGTTGCAGGCGTTGCTTTCTGCGGgct                             >  1:2007778/1‑150 (MQ=255)

                                                                                                                       gtgCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCGCTGACCAGCGTGCTGGTATTTCGCTTTGATGTTGGTGTCGCGCCGCCAGAAATCTTGTTGCAGGCGTTGCTTTCTGCGGgctgc                           >  1:1179272/1‑150 (MQ=255)

                                                                                                                        tgCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCGCTGACCAGCGTGCTGGTATTTCGCTTTGATGTTGGTGTCGCGCCGCCAGAAATCTTGTTGCAGGCGTTGCTTTCTGCGGgctgct                          >  1:885159/1‑150 (MQ=255)

                                                                                                                         gcgcTTTAACTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCGCTGACCAGCGTGCTGGTATTTCGCTTTGATGTTGGTGTCGCGCCGCCAGAAATCTTGTTGCAGGCGTTGCTTTCTGCGGGCTGCtt                         <  1:406144/150‑1 (MQ=255)

                                                                                                                            cTTTAACTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCGCTGACCAGCGTGCTGGTATTTCGCTTTGATGTTGGTGTCGCGCCGCCAGAAATCTTGTTGCAGGCGTTGCTTTCTGCGGGCTGCTTCTa                      <  2:3282717/150‑1 (MQ=255)

                                                                                                                              ttAACTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCGCTGACCAGCGTGCTGGTATTTCGCTTTGATGTTGGTGTCGCGCCGCCAGAAATCTTGTTGCAGGCGTTGCTTTCTGCGGGCTGCTTCTAcg                    >  2:262016/1‑150 (MQ=255)

                                                                                                                              ttAACTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCGCTGACCAGCGTGCTGGTATTTCGCTTTGATGTTGGTGTCGCGCCGCCAGAAATCTTGTTGCAGGCGTTGCTTTCTGCGGGCTGCTTCTAcg                    <  2:4317905/150‑1 (MQ=255)

                                                                                                                                aaCTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCGCTGACCAGCGTGCTGGTATTTCGCTTTGATGTTGGTGTCGCGCCGCCAGAAATCTTGTTGCAGGCGTTGCTTTCTGCGGGCTGCTTCTAcgcg                  >  1:3692609/1‑150 (MQ=255)

                                                                                                                                aaCTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTTTATTCGGCTTCCCGCTGACCAGCGTGCTGGTATTTCGCTTTGATGTTGGTGTCGCGCCGCCAGAAATCTTGTTGCAGGCGTTGCTTTCTGCGGGCTGCTTCTAcgcg                  <  1:1011102/150‑1 (MQ=255)

                                                                                                                                  cTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCGCTGACCAGCGTGCTGGTATTTCGCTTTGATGTTGGTGTCGCGCCGCCAGAAATCTTGTTGCAGGCGTTGCTTTCTGCGGGCTGCTTCTACGCGGt                <  1:4170317/150‑1 (MQ=255)

                                                                                                                                  cTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCGCTGACCAGCGTGCTGGTATTTCGCTTTGATGTTGGTGTCGCGCCGCCAGAAATCTTGTTGCAGGCGTTGCTTTCTGCGGGCTGCTTCTACGCGGt                <  1:934474/150‑1 (MQ=255)

                                                                                                                                   ttCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCGCTGACCAGCGTGCTGGTATTTCGCTTTGATGTTGGTGTCGCGCCGCCAGAAATCTTGTTGCAGGCGTTGCTTTCTGCGGGCTGCTTCTACGCGGtt               >  2:883809/1‑150 (MQ=255)

                                                                                                                                   ttCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCGCTGACCAGCGTGCTGGTATTTCGCTTTGATGTTGGTGTCGCGCCGCCAGAAATCTTGTTGCAGGCGTTGCTTTCTGCGGGCTGCTTCTACGCGGtt               >  1:3989986/1‑150 (MQ=255)

                                                                                                                                    tCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCGCTGACCAGCGTGCTGGTATTTCGCTTTGATGTTGGTGTCGCGCCGCCAGAAATCTTGTTGCAGGCGTTGCTTTCTGCGGGCTGCTTCTACGCGGttt              >  1:1135368/1‑150 (MQ=255)

                                                                                                                                     cAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCGCTGACCAGCGTGCTGGTATTTCGCTTTGATGTTGGTGTCGCGCCGCCAGAAATCTTGTTGCAGGCGTTGCTTTCTGCGGGCTGCTTCTACGCGGTTTa             <  2:3596686/150‑1 (MQ=255)

                                                                                                                                     cAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCGCTGACCAGCGTGCTGGTATTTCGCTTTGATGTTGGTGTCGCGCCGCCAGAAATCTTGTTGCAGGCGTTGCTTTCTGCGGGCTGCTTCTACGCGGTTTa             >  2:4256074/1‑150 (MQ=255)

                                                                                                                                      aaTGTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCGCTGACCAGCGTGCTGGTATTTCGCTTTGATGTTGGTGTCGCGCCGCCAGAAATCTTGTTGCAGGCGTTGCTTTCTGCGGGCTGCTTCTACGCGGTTTAc            >  2:2729040/1‑150 (MQ=255)

                                                                                                                                       aTGTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCGCTGACCAGCGTGCTGGTATTTCGCTTTGATGTTGGTGTCGCGCCGCCAGAAATCTTGTTGCAGGCGTTGCTTTCTGCGGGCTGCTTCTACGCGGTTTACt           >  2:1536084/1‑150 (MQ=255)

                                                                                                                                       aTGTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCGCTGACCAGCGTGCTGGTATTTCGCTTTGATGTTGGTGTCGCGCCGCCAGAAATCTTGTTGCAGGCGTTGCTTTCTGCGGGCTGCTTCTACGCGGTTTACt           >  2:1449083/1‑150 (MQ=255)

                                                                                                                                       aTGTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCGCTGACCAGCGTGCTGGTATTTCGCTTTGATGTTGGTGTCGCGCCGCCAGAAATCTTGTTGCAGGCGTTGCTTTCTGCGGGCTGCTTCTACGCGGTTTACt           >  2:1143885/1‑150 (MQ=255)

                                                                                                                                          tcttctTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCGCTGACCAGCGTGCTGGTATTTCGCTTTGATGTTGGTGTCGCGCCGCCAGAAATCTTGTTGCAGGCGTTGCTTTCTGCGGGCTGCTTCTACGCGGTTTACTATg        >  2:857253/1‑150 (MQ=255)

                                                                                                                                           cttcttTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCGCTGACCAGCGTGCTGGTATTTCGCTTTGATGTTGGTGTCGCGCCGCCAGAAATCTTGTTGCAGGCGTTGCTTTCTGCGGGCTGCTTCTACGCGGTTTACTATGt       <  1:2167368/150‑1 (MQ=255)

                                                                                                                                             tcttTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCGCTGACCAGCGTGCTGGTATTTCGCTTTGATGTTGGTGTCGCGCCGCCAGAAATCTTGTTGCAGGCGTTGCTTTCTGCGGGCTGCTTCTACGCGGTTTACTATGTCa     <  1:184145/150‑1 (MQ=255)

                                                                                                                                                ttttATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCGCTGACCAGCGTGCTGGTATTTCGCTTTGATGTTGGTGTCGCGCCGCCAGAAATCTTGTTGCAGGCGTTGCTTTCTGCGGGCTGCTTCTACGCGGTTTACTATGTCACCt  <  2:3524886/150‑1 (MQ=255)

GCGTTGGCGATTGCCGCCAGGGAGGTCGCATGAGTCTGCTGCAATTCAGTGGCCTGTTTGTTGTCTGGCTGCTCTGCACGCTGTTTATTGCCACGCTGACCTGGTTTGAGTTTCGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTCATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCGCTGACCAGCGTGCTGGTATTTCGCTTTGATGTTGGTGTCGCGCCGCCAGAAATCTTGTTGCAGGCGTTGCTTTCTGCGGGCTGCTTCTACGCGGTTTACTATGTCACCT  >  AP012306/3407785‑3408078

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`