Predicted mutation
evidence seq id position mutation annotation gene description
RA AP012306 3,101,168 C→A S616R (AGC→AGA)  mdtF → multidrug transporter, RpoS‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123063,101,1680CA100.0% 1097.6 / NA 273S616R (AGC→AGA) mdtFmultidrug transporter, RpoS‑dependent
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (139/134);  total (139/134)

Only 100 of 281 total aligned reads displayed.

GGGGGTATTTATGACCACCGCGCAGTTACCTTCCGGTGCCACCATGGTTAACACCACGAAAGTGCTGCAACAGGTGACGGATTATTATCTGACTAAAGAGAAAGATAATGTCCAGTCGGTGTTTACCGTTGGCGGCTTTGGCTTCAGCGGTCAGGGGCAAAACAACGGCCTGGCGTTTATCAGTCTCAAGCCGTGGTCTGAACGTGTCGGTGAGGAAAACTCGGTTACCGCGATCATTCAGCGGGCAATGATTGCGTTAAGCAGTATCAATAAAGCCGTCGTCTTCCCGTTCA  >  AP012306/3101021‑3101313
                                                                                                                                                   |                                                                                                                                                 
gggggTATTTATGACCACCGCGCAGTTACCTTCCGGTGCCACCATGGTTAACACCACGAAAGTGCTGCAACAGGTGACGGATTATTATCTGACTAAAGAGAAAGATAATGTCCAGTCGGTGTTTACCGTTGGCGGCTTTGGCTTCAGAgg                                                                                                                                                 >  1:3700163/1‑150 (MQ=255)
gggggTATTTATGACCACCGCGCAGTTACCTTCCGGTGCCACCATGGTTAACACCACGAAAGTGCTGCAACAGGTGACGGATTATTATCTGACTAAAGAGAAAGATAATGTCCAGTCGGTGTTTACCGTTGGCGGCTTTGGCTTCAGAgg                                                                                                                                                 >  1:4622097/1‑150 (MQ=255)
gggggTATTTATGACCACCGCGCAGTTACCTTCCGGTGCCACCATGGTTAACACCACGAAAGTGCTGCAACAGGTGACGGATTATTATCTGACTAAAGAGAAAGATAATGTCCAGTCGGTGTTTACCGTTGGCGGCTTTGGCTTCAGAgg                                                                                                                                                 <  1:3003115/150‑1 (MQ=255)
 ggggTATTTATGACCACCGCGCAGTTACCTTCCGGTGCCACCATGGTTAACACCACGAAAGTGCTGCAACAGGTGACGGATTATTATCTGACTAAAGAGAAAGATAATGTCCAGTCGGTGTTTACCGTTGGCGGCTTTGGCTTCAGAGGt                                                                                                                                                <  1:2269775/150‑1 (MQ=255)
      aTTTATGACCACCGCGCAGTTACCTTCCGGTGCCACCATGGTTAACACCACGAAAGTGCTGCAACAGGTGACGGATTATTATCTGACTAAAGAGAAAGATAATGTCCAGTCGGTGTTTACCGTTGGCGGCTTTGGCTTCAGAGGTCAggg                                                                                                                                           <  2:3630809/150‑1 (MQ=255)
       tttATGACCACCGCGCAGTTACCTTCCGGTGCCACCATGGTTAACACCACGAAAGTGCTGCAACAGGTGACGGATTATTATCTGACTAAAGAGAAAGATAATGTCCAGTCGGTGTTTACCGTTGGCGGCTTTGGCTTCAGAGGTCAgggg                                                                                                                                          <  1:3154872/150‑1 (MQ=255)
       tttATGACCACCGCGCAGTTACCTTCCGGTGCCACCATGGTTAACACCACGAAAGTGCTGCAACAGGTGACGGATTATTATCTGACTAAAGAGAAAGATAATGTCCAGTCGGTGTTTACCGTTGGCGGCTTTGGCTTCAGAGGTCAgggg                                                                                                                                          >  1:2032987/1‑150 (MQ=255)
             accaccGCGCAGTTACCTTCCGGTGCCACCATGGTTAACACCACGAAAGTGCTGCAACAGGTGACGGATTATTATCTGACTAAAGAGAAAGATAATGTCCAGTCGGTGTTTACCGTTGGCGGCTTTGGCTTCAGAGGTCAGGGGCAAaac                                                                                                                                    <  2:3842010/150‑1 (MQ=255)
             accaccGCGCAGTTACCTTCCGGTGCCACCATGGTTAACACCACGAAAGTGCTGCAACAGGTGACGGATTATTATCTGACTAAAGAGAAAGATAATGTCCAGTCGGTGTTTACCGTTGGCGGCTTTGGCTTCAGAGGTCAGGGGCAAaac                                                                                                                                    <  1:2115361/150‑1 (MQ=255)
               caccGCGCAGTTACCTTCCGGTGCCACCATGGTTAACACCACGAAAGTGCTGCAACAGGTGACGGATTATTATCTGACTAAAGAGAAAGATAATGTCCAGTCGGTGTTTACCGTTGGCGGCTTTGGCTTCAGAGGTCAGGGGCAAaacaa                                                                                                                                  >  2:2810745/1‑150 (MQ=255)
                     gcAGTTACCTTCCGGTGCCACCATGGTTAACACCACGAAAGTGCTGCAACAGGTGACGGATTATTATCTGACTAAAGAGAAAGATAATGTCCAGTCGGTGTTTACCGTTGGCGGCTTTGGCTTCAGAGGTCAGGGGCAAAACAACGGCCt                                                                                                                            <  1:4073603/150‑1 (MQ=255)
                     gcAGTTACCTTCCGGTGCCACCATGGTTAACACCACGAAAGTGCTGCAACAGGTGACGGATTATTATCTGACTAAAGAGAAAGATAATGTCCAGTCGGTGTTTACCGTTGGCGGCTTTGGCTTCAGAGGTCAGGGGCAAAACAACGGCCt                                                                                                                            >  1:4395264/1‑150 (MQ=255)
                       aGTTACCTTCCGGTGCCACCATGGTTAACACCACGAAAGTGCTGCAACAGGTGACGGATTATTATCTGACTAAAGAGAAAGATAATGTCCAGTCGGTGTTTACCGTTGGCGGCTTTGGCTTCAGAGGTCAGGGGCAAAACAACGGCCTgg                                                                                                                          <  1:3030753/150‑1 (MQ=255)
                         ttACCTTCCGGTGCCACCATGGTTAACACCACGAAAGTGCTGCAACAGGTGACGGATTATTATCTGACTAAAGAGAAAGATAATGTCCAGTCGGTGTTTACCGTTGGCGGCTTTGGCTTCAGAGGTCAGGGGCAAAACAACGGCCTGGCg                                                                                                                        >  1:3046489/1‑150 (MQ=255)
                         ttACCTTCCGGTGCCACCATGGTTAACACCACGAAAGTGCTGCAACAGGTGACGGATTATTATCTGACTAAAGAGAAAGATAATGTCCAGTCGGTGTTTACCGTTGGCGGCTTTGGCTTCAGAGGTCAGGGGCAAAACAACGGCCTGGCg                                                                                                                        <  2:1024583/150‑1 (MQ=255)
                           aCCTTCCGGTGCCACCATGGTTAACACCACGAAAGTGCTGCAACAGGTGACGGATTATTATCTGACTAAAGAGAAAGATAATGTCCAGTCGGTGTTTACCGTTGGCGGCTTTGGCTTCAGAGGTCAGGGGCAAAACAACGGCCTGGCGtt                                                                                                                      <  2:1005812/150‑1 (MQ=255)
                             cTTCCGGTGCCACCATGGTTAACACCACGAAAGTGCTGCAACAGGTGACGGATTATTATCTGACTAAAGAGAAAGATAATGTCCAGTCGGTGTTTACCGTTGGCGGCTTTGGCTTCAGAGGTCAGGGGCAAAACAACGGCCTGGCGTTTa                                                                                                                    <  2:3500615/150‑1 (MQ=255)
                                ccGGTGCCACCATGGTTAACACCACGAAAGTGCTGCAACAGGTGACGGATTATTATCTGACTAAAGAGAAAGATAATGTCCAGTCGGTGTTTACCGTTGGCGGCTTTGGCTTCAGAGGTCAGGGGCAAAACAACGGCCTGGCGTTTATCa                                                                                                                 >  1:74087/1‑150 (MQ=255)
                                  ggTGCCACCATGGTTAACACCACGAAAGTGCTGCAACAGGTGACGGATTATTATCTGACTAAAGAGAAAGATAATGTCCAGTCGGTGTTTACCGTTGGCGGCTTTGGCTTCAGAGGTCAGGGGCAAAACAACGGCCTGGCGTTTATCAGt                                                                                                               >  1:3256303/1‑150 (MQ=255)
                                   gTGCCACCATGGTTAACACCACGAAAGTGCTGCAACAGGTGACGGATTATTATCTGACTAAAGAGAAAGATAATGTCCAGTCGGTGTTTACCGTTGGCGGCTTTGGCTTCAGAGGTCAGGGGCAAAACAACGGCCTGGCGTTTATCAGtc                                                                                                              <  2:4503367/150‑1 (MQ=255)
                                    tGCCACCATGGTTAACACCACGAAAGTGCTGCAACAGGTGACGGATTATTATCTGACTAAAGAGAAAGATAATGTCCAGTCGGTGTTTACCGTTGGCGGCTTTGGCTTCAGAGGTCAGGGGCAAAACAACGGCCTGGCGTTTATCAGtct                                                                                                             <  2:2180216/150‑1 (MQ=255)
                                    tGCCACCATGGTTAACACCACGAAAGTGCTGCAACAGGTGACGGATTATTATCTGACTAAAGAGAAAGATAATGTCCAGTCGGTGTTTACCGTTGGCGGCTTTGGCTTCAGAGGTCAGGGGCAAAACAACGGCCTGGCGTTTATCAGtct                                                                                                             >  2:2672811/1‑150 (MQ=255)
                                    tGCCACCATGGTTAACACCACGAAAGTGCTGCAACAGGTGACGGATTATTATCTGACTAAAGAGAAAGATAATGTCCAGTCGGTGTTTACCGTTGGCGGCTTTGGCTTCAGAGGTCAGGGGCAAAACAACGGCCTGGCGTTTATCAGtct                                                                                                             >  2:2853142/1‑150 (MQ=255)
                                    tGCCACCATGGTTAACACCACGAAAGTGCTGCAACAGGTGACGGATTATTATCTGACTAAAGAGAAAGATAATGTCCAGTCGGTGTTTACCGTTGGCGGCTTTGGCTTCAGAGGTCAGGGGCAAAACAACGGCCTGGCGTTTATCAGtct                                                                                                             >  1:1387750/1‑150 (MQ=255)
                                      ccaccaTGGTTAACACCACGAAAGTGCTGCAACAGGTGACGGATTATTATCTGACTAAAGAGAAAGATAATGTCCAGTCGGTGTTTACCGTTGGCGGCTTTGGCTTCAGAGGTCAGGGGCAAAACAACGGCCTGGCGTTTATCAGTCTCa                                                                                                           >  2:2063815/1‑150 (MQ=255)
                                       caccaTGGTTAACACCACGAAAGTGCTGCAACAGGTGACGGATTATTATCTGACTAAAGAGAAAGATAATGTCCAGTCGGTGTTTACCGTTGGCGGCTTTGGCTTCAGAGGTCAGGGGCAAAACAACGGCCTGGCGTTTATCAGTCTCaa                                                                                                          >  1:3526614/1‑150 (MQ=255)
                                       caccaTGGTTAACACCACGAAAGTGCTGCAACAGGTGACGGATTATTATCTGACTAAAGAGAAAGATAATGTCCAGTCGGTGTTTACCGTTGGCGGCTTTGGCTTCAGAGGTCAGGGGCAAAACAACGGCCTGGCGTTTATCAGTCTCaa                                                                                                          >  1:4299743/1‑150 (MQ=255)
                                           aTGGTTAACACCACGAAAGTGCTGCAACAGGTGACGGATTATTATCTGACTAAAGAGAAAGATAATGTCCAGTCGGTGTTTACCGTTGGCGGCTTTGGCTTCAGAGGTCAGGGGCAAAACAACGGCCTGGCGTTTATCAGTCTCAAGCCg                                                                                                      >  2:3163794/1‑150 (MQ=255)
                                            tGGTTAACACCACGAAAGTGCTGCAACAGGTGACGGATTATTATCTGACTAAAGAGAAAGATAATGTCCAGTCGGTGTTTACCGTTGGCGGCTTTGGCTTCAGAGGTCAGGGGCAAAACAACGGCCTGGCGTTTATCAGTCTCAAGCCGt                                                                                                     >  2:4485714/1‑150 (MQ=255)
                                                   caccacGAAAGTGCTGCAACAGGTGACGGATTATTATCTGACTAAAGAGAAAGATAATGTCCAGTCGGTGTTTACCGTTGGCGGCTTTGGCTTCAGAGGTCAGGGGCAAAACAACGGCCTGGCGTTTATCAGTCTCAAGCCGTGGTCTGa                                                                                              >  2:590659/1‑150 (MQ=255)
                                                     ccacGAAAGTGCTGCAACAGGTGACGGATTATTATCTGACTAAAGAGAAAGATAATGTCCAGTCGGTGTTTACCGTTGGCGGCTTTGGCTTCAGAGGTCAGGGGCAAAACAACGGCCTGGCGTTTATCAGTCTCAAGCCGTGGTCTGAAc                                                                                            <  1:1752376/150‑1 (MQ=255)
                                                     ccacGAAAGTGCTGCAACAGGTGACGGATTATTATCTGACTAAAGAGAAAGATAATGTCCAGTCGGTGTTTACCGTTGGCGGCTTTGGCTTCAGAGGTCAGGGGCAAAACAACGGCCTGGCGTTTATCAGTCTCAAGCCGTGGTCTGAAc                                                                                            <  1:4288258/150‑1 (MQ=255)
                                                       acGAAAGTGCTGCAACAGGTGACGGATTATTATCTGACTAAAGAGAAAGATAATGTCCAGTCGGTGTTTACCGTTGGCGGCTTTGGCTTCAGAGGTCAGGGGCAAAACAACGGCCTGGCGTTTATCAGTCTCAAGCCGTGGTCTGAACgt                                                                                          >  1:517919/1‑150 (MQ=255)
                                                        cGAAAGTGCTGCAACAGGTGACGGATTATTATCTGACTAAAGAGAAAGATAATGTCCAGTCGGTGTTTACCGTTGGCGGCTTTGGCTTCAGAGGTCAGGGGCAAAACAACGGCCTGGCGTTTATCAGTCTCAAGCCGTGGTCTGAACgtg                                                                                         >  2:1488931/1‑150 (MQ=255)
                                                           aaGTGCTGCAACAGGTGACGGATTATTATCTGACTAAAGAGAAAGATAATGTCCAGTCGGTGTTTACCGTTGGCGGCTTTGGCTTCAGAGGTCAGGGGCAAAACAACGGCCTGGCGTTTATCAGTCTCAAGCCGTGGTCTGAACGTGTCg                                                                                      <  1:3074274/150‑1 (MQ=255)
                                                            aGTGCTGCAACAGGTGACGGATTATTATCTGACTAAAGAGAAAGATAATGTCCAGTCGGTGTTTACCGTTGGCGGCTTTGGCTTCAGAGGTCAGGGGCAAAACAACGGCCTGGCGTTTATCAGTCTCAAGCCGTGGTCTGAACGTGTCgg                                                                                     >  1:3976235/1‑150 (MQ=255)
                                                             gTGCTGCAACAGGTGACGGATTATTATCTGACTAAAGAGAAAGATAATGTCCAGTCGGTGTTTACCGTTGGCGGCTTTGGCTTCAGAGGTCAGGGGCAAAACAACGGCCTGGCGTTTATCAGTCTCAAGCCGTGGTCTGAACGTGTCGGt                                                                                    <  2:2153778/150‑1 (MQ=255)
                                                               gctgcAACAGGTGACGGATTATTATCTGACTAAAGAGAAAGATAATGTCCAGTCGGTGTTTACCGTTGGCGGCTTTGGCTTCAGAGGTCAGGGGCAAAACAACGGCCTGGCGTTTATCAGTCTCAAGCCGTGGTCTGAACGTGTCGGTGa                                                                                  <  2:1330076/150‑1 (MQ=255)
                                                                 tgcAACAGGTGACGGATTATTATCTGACTAAAGAGAAAGATAATGTCCAGTCGGTGTTTACCGTTGGCGGCTTTGGCTTCAGAGGTCAGGGGCAAAACAACGGCCTGGCGTTTATCAGTCTCAAGCCGTGGTCTGAACGTGTCGGTGAgg                                                                                <  1:106926/150‑1 (MQ=255)
                                                                  gcAACAGGTGACGGATTATTATCTGACTAAAGAGAAAGATAATGTCCAGTCGGTGTTTACCGTTGGCGGCTTTGGCTTCAGAGGTCAGGGGCAAAACAACGGCCTGGCGTTTATCAGTCTCAAGCCGTGGTCTGAACGTGTCGGTGAGGa                                                                               <  1:1120261/150‑1 (MQ=255)
                                                                  gcAACAGGTGACGGATTATTATCTGACTAAAGAGAAAGATAATGTCCAGTCGGTGTTTACCGTTGGCGGCTTTGGCTTCAGAGGTCAGGGGCAAAACAACGGCCTGGCGTTTATCAGTCTCAAGCCGTGGTCTGAACGTGTCGGTGAGGa                                                                               >  2:2923381/1‑150 (MQ=255)
                                                                      cAGGTGACGGATTATTATCTGACTAAAGAGAAAGATAATGTCCAGTCGGTGTTTACCGTTGGCGGCTTTTGCTTCAGAGGTCAGGGGCAAAACAACGGCCTGGCGTTTATCAGTCTCAAGCCGTGGTCTGAACGTGTCGGTGAGGAAAAc                                                                           >  1:4496353/1‑150 (MQ=255)
                                                                       aGGTGACGGATTATTATCTGACTAAAGAGAAAGATAATGTCCAGTCGGTGTTTACCGTTGGCGGCTTTGGCTTCAGAGGTCAGGGGCAAAACAACGGCCTGGCGTTTATCAGTCTCAAGCCGTGGTCTGAACGTGTCGGTGAGGAAAACt                                                                          >  1:1971693/1‑150 (MQ=255)
                                                                         gTGACGGATTATTATCTGACTAAAGAGAAAGATAATGTCCAGTCGGTGTTTACCGTTGGCGGCTTTGGCTTCAGAGGTCAGGGGCAAAACAACGGCCTGGCGTTTATCAGTCTCAAGCCGTGGTCTGAACGTGTCGGTGAGGAAAACTCg                                                                        >  2:3025911/1‑150 (MQ=255)
                                                                         gTGACGGATTATTATCTGACTAAAGAGAAAGATAATGTCCAGTCGGTGTTTACCGTTGGCGGCTTTGGCGTTAGAGGTGAGGGGCAAAATAACAGCCTGGTGTTTATCAGTTTCAAGCCGTGGTCTGAAAGTGTCTGTGAGTAAAAcatg                                                                        >  2:4377761/1‑147 (MQ=255)
                                                                           gACGGATTATTATCTGACTAAAGAGAAAGATAATGTCCAGTCGGTGTTTACCGTTGGCGGCTTTGGCTTCAGAGGTCAGGGGCAAAACAACGGCCTGGCGTTTATCAGTCTCAAGCCGTGGTCTGAACGTGTCGGTGAGGAAAACTCGGt                                                                      >  2:2338535/1‑150 (MQ=255)
                                                                            aCGGATTATTATCTGACTAAAGAGAAAGATAATGTCCAGTCGGTGTTTACCGTTGGCGGCTTTGGCTTCAGAGGTCAGGGGCAAAACAACGGCCTGGCGTTTATCAGTCTCAAGCCGTGGTCTGAACGTGTCGGTGAGGAAAACTCGGtt                                                                     >  1:1640203/1‑150 (MQ=255)
                                                                              ggATTATTATCTGACTAAAGAGAAAGATAATGTCCAGTCGGTGTTTACCGTTGGCGGCTTTGGCTTCAGAGGTCAGGGGCAAAACAACGGCCTGGCGTTTATCAGTCTCAAGCCGTGGTCTGAACGTGTCGGTGAGGAAAACTCGGTTAc                                                                   <  2:4173602/150‑1 (MQ=255)
                                                                              ggATTATTATCTGACTAAAGAGAAAGATAATGTCCAGTCGGTGTTTACCGTTGGCGGCTTTGGCTTCAGAGGTCAGGGGCAAAACAACGGCCTGGCGTTTATCAGTCTCAAGCCGTGGTCTGAACGTGTCGGTGAGGAAAACTCGGTTAc                                                                   >  2:736925/1‑150 (MQ=255)
                                                                                 ttattaTCTGACTAAAGAGAAAGATAATGTCCAGTCGGTGTTTACCGTTGGCGGCTTTGGCTTCAGAGGTCAGGGGCAAAACAACGGCCTGGCGTTTATCAGTCTCAAGCCGTGGTCTGAACGTGTCGGTGAGGAAAACTCGGTTACcgc                                                                <  1:2556958/150‑1 (MQ=255)
                                                                                   attatCTGACTAAAGAGAAAGATAATGTCCAGTCGGTGTTTACCGTTGGCGGCTTTGGCTTCAGAGGTCAGGGGCAAAACAACGGCCTGGCGTTTATCAGTCTCAAGCCGTGGTCTGAACGTGTCGGTGAGGAAAACTCGGTTACCGCGa                                                              >  1:63364/1‑150 (MQ=255)
                                                                                   attatCTGACTAAAGAGAAAGATAATGTCCAGTCGGTGTTTACCGTTGGCGGCTTTGGCTTCAGAGGTCAGGGGCAAAACAACGGCCTGGCGTTTATCAGTCTCAAGCCGTGGTCTGAACGTGTCGGTGAGGAAAACTCGGTTACCGCGa                                                              >  1:3336490/1‑150 (MQ=255)
                                                                                    ttatCTGACTAAAGAGAAAGATAATGTCCAGTCGGTGTTTACCGTTGGCGGCTTTGGCTTCAGAGGTCAGGGGCAAAACAACGGCCTGGCGTTTATCAGTCTCAAGCCGTGGTCTGAACGTGTCGGTGAGGAAAACTCGGTTACCGCGAt                                                             <  1:2749784/150‑1 (MQ=255)
                                                                                    ttatCTGACTAAAGAGAAAGATAATGTCCAGTCGGTGTTTACCGTTGGCGGCTTTGGCTTCAGAGGTCAGGGGCAAAACAACGGCCTGGCGTTTATCAGTCTCAAGCCGTGGTCTGAACGTGTCGGTGAGGAAAACTCGGTTACCGCGAt                                                             >  2:4147572/1‑150 (MQ=255)
                                                                                     tatCTGACTAAAGAGAAAGATAATGTCCAGTCGGTGTTTACCGTTGGCGGCTTTGGCTTCAGAGGTCAGGGGCAAAACAACGGCCTGGCGTTTATCAGTCTCAAGCCGTGGTCTGAACGTGTCGGTGAGGAAAACTCGGTTACCGCGATc                                                            >  1:979842/1‑150 (MQ=255)
                                                                                     tatCTGACTAAAGAGAAAGATAATGTCCAGTCGGTGTTTACCGTTGGCGGCTTTGGCTTCAGAGGTCAGGGGCAAAACAACGGACTGGCGTTTATCAGTCTCAAGCCGTGGTCTGAACGTGTCGGTGAGGAAAACTCGGTTACCGCGATc                                                            <  1:282037/150‑1 (MQ=255)
                                                                                       tCTGACTAAAGAGAAAGATAATGTCCAGTCGGTGTTTACCGTTGGCGGCTTTGGCTTCAGAGGTCAGGGGCAAAACAACGGCCTGGCGTTTATCAGTCTCAAGCCGTGGTCTGAACGTGTCGGTGAGGAAAACTCGGTTACCGCGATCAt                                                          <  1:2352826/150‑1 (MQ=255)
                                                                                       tCTGACTAAAGAGAAAGATAATGTCCAGTCGGTGTTTACCGTTGGCGGCTTTGGCTTCAGAGGTCAGGGGCAAAACAACGGCCTGGCGTTTATCAGTCTCAAGCCGTGGTCTGAACGTGTCGGTGAGGAAAACTCGGTTACCGCGATCAt                                                          >  2:4411975/1‑150 (MQ=255)
                                                                                        cTGACTAAAGAGAAAGATAATGTCCAGTCGGTGTTTACCGTTGGCGGCTTTGGCTTCAGAGGTCAGGGGCAAAACAACGGCCTGGCGTTTATCAGTCTCAAGCCGTGGTCTGAACGTGTCGGTGAGGAAAACTCGGTTACCGCGATCAtt                                                         >  1:1874664/1‑150 (MQ=255)
                                                                                         tGACTAAAGAGAAAGATAATGTCCAGTCGGTGTTTACCGTTGGCGGCTTTGGCTTCAGAGGTCAGGGGCAAAACAACGGCCTGGCGTTTATCAGTCTCAAGCCGTGGTCTGAACGTGTCGGTGAGGAAAACTCGGTTACCGCGATCATTc                                                        >  1:3876207/1‑150 (MQ=255)
                                                                                            cTAAAGAGAAAGATAATGTCCAGTCGGTGTTTACCGTTGGCGGCTTTGGCTTCAGAGGTCAGGGGCAAAACAACGGCCTGGCGTTTATCAGTCTCAAGCCGTGGTCTGAACGTGTCGGTGAGGAAAACTCGGTTACCGCGATCATTCAGc                                                     <  1:181178/150‑1 (MQ=255)
                                                                                            cTAAAGAGAAAGATAATGTCCAGTCGGTGTTTACCGTTGGCGGCTTTGGCTTCAGAGGTCAGGGGCAAAACAACGGCCTGGCGTTTATCAGTCTCAAGCCGTGGTCTGAACGTGTCGGTGAGGAAAACTCGGTTACCGCGATCATTCAGc                                                     <  1:3834742/150‑1 (MQ=255)
                                                                                            cTAAAGAGAAAGATAATGTCCAGTCGGTGTTTACCGTTGGCGGCTTTGGCTTCAGAGGTCAGGGGCAAAACAACGGCCTGGCGTTTATCAGTCTCAAGCCGTGGTCTGAACGTGTCGGTGAGGAAAACTCGGTTACCGCGATCATTCAGc                                                     <  2:2564345/150‑1 (MQ=255)
                                                                                             tAAAGAGAAAGATAATGTCCAGTCGGGGTTTACCGTTGGCGGCTTTGGCTTCAGAGGTCAGGGGCAAAACAACGGCCTGGCGTTTATCAGTCTCAAGCCGTGGTCTGAACGTGTCGGTGAGGAAAACTCGGTTACCGCGATCATTCAGCg                                                    >  2:345057/1‑150 (MQ=255)
                                                                                                  agaAAGATAATGTCCAGTCGGTGTTTACCGTTGGCGGCTTTGGCTTCAGAGGTCAGGGGCAAAACAACGGCCTGGCGTTTATCAGTCTCAAGCCGTGGTCTGAACGTGTCGGTGAGGAAAACTCGGTTACCGCGATCATTCAGCGGGCaa                                               >  2:808778/1‑150 (MQ=255)
                                                                                                  agaAAGATAATGTCCAGTCGGTGTTTACCGTTGGCGGCTTTGGCTTCAGAGGTCAGGGGCAAAACAACGGCCTGGCGTTTATCAGTCTCAAGCCGTGGTCTGAACGTGTCGGTGAGGAAAACTCGGTTACCGCGATCATTCAGCGGGCaa                                               <  2:2507695/150‑1 (MQ=255)
                                                                                                     aaGATAATGTCCAGTCGGTGTTTACCGTTGGCGGCTTTGGCTTCAGAGGTCAGGGGCAAAACAACGGCCTGGCGTTTATCAGTCTCAAGCCGTGGTCTGAACGTGTCGGTGAGGAAAACTCGGTTACCGCGATCATTCAGCGGGCAATGa                                            >  2:4020173/1‑150 (MQ=255)
                                                                                                     aaGATAATGTCCAGTCGGTGTTTACCGTTGGCGGCTTTGGCTTCAGAGGTCAGGGGCAAAACAACGGCCTGGCGTTTATCAGTCTCAAGCCGTGGTCTGAACGTGTCGGTGAGGAAAACTCGGTTACCGCGATCATTCAGCGGGCAATGa                                            >  1:1253812/1‑150 (MQ=255)
                                                                                                          aaTGTCCAGTCGGTGTTTACCGTTGGCGGCTTTGGCTTCAGAGGTCAGGGGCAAAACAACGGCCTGGCGTTTATCAGTCTCAAGCCGTGGTCTGAACGTGTCGGTGAGGAAAACTCGGTTACCGCGATCATTCAGCGGGCAATGATTGCg                                       >  1:3408905/1‑150 (MQ=255)
                                                                                                          aaTGTCCAGTCGGTGTTTACCGTTGGCGGCTTTGGCTTCAGAGGTCAGGGGCAAAACAACGGCCTGGCGTTTATCAGTCTCAAGCCGTGGTCTGAACGTGTCGGTGAGGAAAACTCGGTTACCGCGATCATTCAGCGGGCAATGATTGCg                                       >  2:4343108/1‑150 (MQ=255)
                                                                                                           aTGTCCAGTCGGTGTTTACCGTTGGCGGCTTTGGCTTCAGAGGTCAGGGGCAAAACAACGGCCTGGCGTTTATCAGTCTCAAGCCGTGGTCTGAACGGGTCGGTGAGGAAAACTCGGTTACCGCGGTCATTCCGCGGGCAATGATTGCGt                                      >  2:872225/1‑150 (MQ=255)
                                                                                                            tGTCCAGTCGGTGTTTACCGTTGGCGGCTTTGGCTTCAGAGGTCAGGGGCAAAACAACGGCCTGGCGTTTATCAGTCTCAAGCCGTGGTCTGAACGTGTCGGTGAGGAAAACTCGGTTACCGCGATCATTCAGCGGGCAATGATTGCGtt                                     <  2:1633605/150‑1 (MQ=255)
                                                                                                            tGTCCAGACGGTGTTTACCGTTGGCGGCTTTGGCTTCAGAGGTCAGGGGCAAAACAACGGCCTGGCGTTTATCAGTCTCAAGCCGTGGTCTGAACGTGTCGGTGAGGAAAACTCGGTTACCGCGATCATTCAGCGGGCAATGATTGCGtt                                     <  2:4048035/150‑1 (MQ=255)
                                                                                                             gTCCAGTCGGTGTTTACCGTTGGCGGCTTTGGCTTCAGAGGTCAGGGGCAAAACAACGGCCTGGCGTTTATCAGTCTCAAGCCGTGGTCTGAACGTGTCGGTGAGGAAAACTCGGTTACCGCGATCATTCAGCGGGCAATGATTGCGTTa                                    >  2:374794/1‑150 (MQ=255)
                                                                                                              tCCAGTCGGTGTTTACCGTTGGCGGCTTTGGCTTCAGAGGTCAGGGGCAAAACAACGGCCTGGCGTTTATCAGTCTCAAGCCGTGGTCTGAACGTGTCGGTGAGGAAAACTCGGTTACCGCGATCATTCAGCGGGCAATGATTGCGTTaa                                   >  2:3077136/1‑150 (MQ=255)
                                                                                                                cAGTCGGTGTTTACCGTTGGCGGCTTTGGCTTCAGAGGTCAGGGGCAAAACAACGGCCTGGCGTTTATCAGTCTCAAGCCGTGGTCTGAACGTGTCGGTGAGGAAAACTCGGTTACCGCGATCATTCAGCGGGCAATGATTGCGTTAAGc                                 >  1:1143075/1‑150 (MQ=255)
                                                                                                                cAGTCGGTGTTTACCGTTGGCGGCTTTGGCTTCAGAGGTCAGGGGCAAAACAACGGCCTGGCGTTTATCAGTCTCAAGCCGTGGTCTGAACGTGTCGGTGAGGAAAACTCGGTTACCGCGATCATTCAGCGGGCAATGATTGCGTTAAGc                                 >  1:2409661/1‑150 (MQ=255)
                                                                                                                  gTCGGTGTTTACCGTTGGCGGCTTTGGCTTCAGAGGTCAGGGGCAAAACAACGGCCTGGCGTTTATCAGTCTCAAGCCGTGGTCTGAACGTGTCGGTGAGGAAAACTCGGTTACCGCGATCATTCAGCGGGCAATGATTGCGTTAAGCAg                               <  1:3454236/150‑1 (MQ=255)
                                                                                                                   tCGGTGTTTACCGTTGGCGGCTTTGGCTTCAGAGGTCAGGGGCAAAACAACGGCCTGGCGTTTATCAGTCTCAAGCCGTGGTCTGAACGTGTCGGTGAGGAAAACTCGGTTACCGCGATCATTCAGCGGGCAATGATTGCGGTaaccgag                              <  2:517919/150‑6 (MQ=255)
                                                                                                                    cGGTGTTTACCGTTGGCGGCTTTGGCTTCAGAGGTCAGGGGCAAAACAACGGCCTGGCGTTTATCAGTCTCAAGCCGTGGTCTGAACGTGTCGGTGAGGAAAACTCGGTTACCGCGATCATTCAGCGGGCAATGATTGCGTTAAGCAGTa                             <  2:152463/150‑1 (MQ=255)
                                                                                                                     ggTGTTTACCGTTGGCGGCTTTGGCTTCAGAGGTCAGGGGCAAAACAACGGCCTGGCGTTTATCAGTCTCAAGCCGTGGTCTGAACGTGTCGGTGAGGAAAACTCGGTTACCGCGATCATTCAGCGGGCAATGATTGCGTTAAGCAGTAt                            <  2:496977/150‑1 (MQ=255)
                                                                                                                       tgtTTACCGTTGGCGGCTTTGGCTTCAGAGGTCAGGGGCAAAACAACGGCCTGGCGTTTATCAGTCTCAAGCCGTGGTCTGAACGTGTCGGTGAGGAAAACTCGGTTACcgc                                                                <  2:458556/112‑1 (MQ=255)
                                                                                                                       tgtTTACCGTTGGCGGCTTTGGCTTCAGAGGTCAGGGGCAAAACAACGGCCTGGCGTTTATCAGTCTCAAGCCGTGGTCTGAACGTGTCGGTGAGGAAAACTCGGTTACcgc                                                                >  1:458556/1‑112 (MQ=255)
                                                                                                                       tgtTTACCGTTGGCGGCTTTGGCTTCAGAGGTCAGGGGCAAAACAACGGCCTGGCGTTTATCAGTCTCAAGCCGTGGTCTGAACGTGTCGGTGAGGAAAACTCGGTTACCGCGATCATTCAGCGGGCAATGATTGCGTTAAGCAGTATCa                          >  1:891389/1‑150 (MQ=255)
                                                                                                                        gtTTACCGTTGGCGGCTTTGGCTTCAGAGGTCAGGGGCAAAACAACGGCCTGGCGTTTATCAGTCTCAAGCCGTGGTCTGAACGTGTCGGTGAGGAAAACTCGGTTACCGCGATCATTCAGCGGGCAATGATTGCGTTAAGCAGTATCaa                         <  2:2009289/150‑1 (MQ=255)
                                                                                                                        gtTTACCGTTGGCGGCTTTGGCTTCAGAGGTCAGGGGCAAAACAACGGCCTGGCGTTTATCAGTCTCAAGCCGTGGTCTGAACGTGTCGGTGAGGAAAACTCGGTTACCGCGATCATTCAGCGGGCAATGATTGCGTTAAGCAGTATCaa                         <  2:1120782/150‑1 (MQ=255)
                                                                                                                         tttACCGTTGGCGGCTTTGGCTTCAGAGGTCAGGGGCAAAACAACGGCCTGGCGTTTATCAGTCTCAAGCCGTGGTCTGAACGTGTCGGTGAGGAAAACTCGGTTACCGCGATCATTCAGCGTGCAATGATTGCGTTAAGCAGTATCAAt                        >  1:1055772/1‑150 (MQ=255)
                                                                                                                         tttACCGTTGGCGGCTTTGGCTTCAGAGGTCAGGGGCAAAACAACGGCCTGGCGTTTATCAGTCTCAAGCCGTGGTCTGAACGTGTCGGTGAGGAAAACTCGGTTACCGCGATCATTCAGCGGGCAATGATTGCGTTAAGCAGTATCAAt                        >  1:4003732/1‑150 (MQ=255)
                                                                                                                           tACCGTTGGCGGCTTTGGCTTCAGAGGTCAGGGGCAAAACAACGGCCTGGCGTTTATCAGTCTCAAGCCGTGGTCTGAACGTGTCGGTGAGGAAAACTCGGTTACCGCGATCATTCAGCGGGCAATGATTGCGTTAAGCAGTATCAATaa                      <  1:2598759/150‑1 (MQ=255)
                                                                                                                           tACCGTTGGCGGCTTTGGCTTCAGAGGTCAGGGGCAAAACAACGGCCTGGCGTTTATCAGTCTCAAGCCGTGGTCTGAACGTGTCGGTGAGGAAAACTCGGTTACCGCGATCATTCAGCGGGCAATGATTGCGTTAAGCAGTATCAATaa                      <  1:2504803/150‑1 (MQ=255)
                                                                                                                            aCCGTTGGCGGCTTTGGCTTCAGAGGTCAGGGGCAAAACAACGGCCTGGCGTTTATCAGTCTCAAGCCGTGGTCTGAACGTGTCGGTGAGGAAAACTCGGTTACCGCGATCATTCAGCGGGCAATGATTGCGTTAAGCAGTATCAATaaa                     <  1:2856411/150‑1 (MQ=255)
                                                                                                                              cGTTGGCGGCTTTGGCTTCAGAGGTCAGGGGCAAAACAACGGCCTGGCGTTTATCAGTCTCAAGCCGTGGTCTGAACGTGTCGGTGAGGAAAACTCGGTTACCGCGATCATTCAGCGGGCAATGATTGCGTTAAGCAGTATCAATAAAGc                   >  1:1482477/1‑150 (MQ=255)
                                                                                                                                  ggcggcTTTGGCTTCAGAGGTCAGGGGCAAAACAACGGCCTGGCGTTTATCAGTCTCAAGCCGTGGTCTGAACGTGTCGGTGAGGAAAACTCGGTTACCGCGATCATTCAGCGGGCAATGATTGCGTTAAGCAGTATCAATAAAGCcgtc               <  2:3220275/150‑1 (MQ=255)
                                                                                                                                  ggcggcTTTGGCTTCAGAGGTCAGGGGCAAAACAACGGCCTGGCGTTTATCAGTCTCAAGCCGTGGTCTGAACGTGTCGGTGAGGAAAACTCGGTTACCGCGATCATTCAGCGGGCAATGATTGCGTTAAGCAGTATCAATAAAGCcgtc               <  2:1797073/150‑1 (MQ=255)
                                                                                                                                   gcggcTTTGGCTTCAGAGGTCAGGGGCAAAACAACGGCCTGGCGTTTATCAGTCTCAAGCCGTGGTCTGAACGTGTCGGTGAGGAAAACTCGGTTACCGCGATCATTCAGCGGGCAATGATTGCGTTAAGCAGTATCAATAAAGCcgtcg              <  2:153563/150‑1 (MQ=255)
                                                                                                                                     ggcTTTGGCTTCAGAGGTCAGGGGCAAAACAACGGCCTGGCGTTTATCAGTCTCAAGCCGTGGTCTGAACGTGTCGGTGAGGAAAACTCGGTTACCGCGATCATTCAGCGGGCAATGATTGCGTTAAGCAGTATCAATAAAGCCgtcgtc            >  2:3291892/1‑150 (MQ=255)
                                                                                                                                       cTTTGGCTTCAGAGGTCAGGGGCAAAACAACGGCCTGGCGTTTATCAGTCTCAAGCCGTGGTCTGAACGTGTCGGTGAGGAAAACTCGGTTACCGCGATCATTCAGCGGGCAATGATTGCGTTAAGCAGTATCAATAAAGCCGTCGTCtt          <  2:3928956/150‑1 (MQ=255)
                                                                                                                                          tGGCTTCAGAGGTCAGGGGCAAAACAACGGCCTGGCGTTTATCAGTCTCAAGCCGTGGTCTGAACGTGTCGGTGAGGAAAACTCGGTTACCGCGATCATTCAGCGGGCAATGATTGCGTTAAGCAGTATCAATAAAGCCGTCGTCTTccc       <  1:3896947/150‑1 (MQ=255)
                                                                                                                                             cTTCAGAGGTCAGGGGCAAAACAACGGCCTGGCGTTTATCAGTCTCAAGCCGTGGTCTGAACGTGTCGGTGAGGAAAACTCGGTTACCGCGATCATTCAGCGGGCAATGATTGCGTTAAGCAGTATCAATAAAGCCGTCGTCTTCCCGtt    <  1:1605484/150‑1 (MQ=255)
                                                                                                                                               tCAGAGGTCAGGGGCAAAACAACGGCCTGGCGTTTATCAGTCTCAAGCCGTGGTCTGAACGTGTCGGTGAGGAAAACTCGGTTACCGCGATCATTCAGCGGGCAATGATTGCGTTAAGCAGTATCAATAAAGCCGTCGTCTTCCCGTTCa  >  2:2257136/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
GGGGGTATTTATGACCACCGCGCAGTTACCTTCCGGTGCCACCATGGTTAACACCACGAAAGTGCTGCAACAGGTGACGGATTATTATCTGACTAAAGAGAAAGATAATGTCCAGTCGGTGTTTACCGTTGGCGGCTTTGGCTTCAGCGGTCAGGGGCAAAACAACGGCCTGGCGTTTATCAGTCTCAAGCCGTGGTCTGAACGTGTCGGTGAGGAAAACTCGGTTACCGCGATCATTCAGCGGGCAATGATTGCGTTAAGCAGTATCAATAAAGCCGTCGTCTTCCCGTTCA  >  AP012306/3101021‑3101313

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑