New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? AP012306 = 96920334 (0.960)8 (0.030) 8/274 11.6 2.5% coding (1331/1476 nt) murC UDP‑N‑acetylmuramate:L‑alanine ligase
?AP012306 = 96908 305 (0.960)coding (1319/1476 nt) murC UDP‑N‑acetylmuramate:L‑alanine ligase
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

TATGATGATTTCGCCAATGTGCTGACGCAGGTTGATACCCTGTTGATGCTGGAAGTGTATCCGGCTGGCGAAGCGCCAATTCCGGGAGCGGACAGCCGTTCGCTGTGTCGCACAATTCGTGGACGTGGGAAAATTGATCCCATTCTGGTGCCGGATCCGGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  AP012306/96760‑96920
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gccggatccggcACCAGAATGGGATCAATTTTCCCACGTCCACGAATTGTGCGACACAGCGAACGGCTGTCCGCTCCCGGAATTGGCGCTTCGCCAGCCGGATACACTTCCAGCATCAACAGGGTATCAACCTGCGTCAGCACATTGGCGAAATCATCAT  <  AP012306/96908‑96761
                                                                                                                                                                                                                                                                                                                     
TATGATGATTTCGCCAATGTGCTGACGCAGGTTGATACCCTGTTGATGCTGGAAGTGTATCCGGCTGGCGAAGCGCCAATTCCGGGAGCGGACGGCCGTTCGCTGTGTCGCACAATTCGTGGACGTGGGAAAATTGATCCCATTCTGGTG                                                                                                                                                                 <  1:4751773/150‑1
  TGATGATTTCGCCAATGTGCTGACGCAGGTTGATACCCTGTTGATGCTGGAAGTGTATCCGGCTGGCGAAGCGCCAATTCCGGGAGCGGACAGCGGTTCGCTGTGTCGCACAATTCGTGGACGTGGGAAAATTGATCCCATTCTGGTGCC                                                                                                                                                               >  1:4434543/1‑150
    ATGATTTCGCCAATGTGCTGACGCAGGTTGATACCCTGTTGATGCTGGAAGTGTATCCGGCTGGCGAAGCGCCAATTCCGGGAGCGGACAGCCGTTCGCTGTGTCGCACAATTCGTGGACGTGGGAAAATTGATCCCATTCTGGTGCCGG                                                                                                                                                             >  2:3558713/1‑150
    ATGATTTCGCCAATGTGCTGACGCAGGTTGATACCCTGTTGATGCTGGAAGTGTATCCGGCTGGCGAAGCGCCAATTCCGGGAGCGGACAGCCGTTCGCTGTGTCGCACAATTCGTGGACGTGGGAAAATTGATCCCATTCTGGTGCCGG                                                                                                                                                             >  1:1340833/1‑150
     TGATTTCGCCAATGTGCTGACGCAGGTTGATACCCTGTTGATGCTGGAAGTGTATCCGGCTGGCGAAGCGCCAATTCCGGGAGCGGACAGCCGTTCGCTGTGTCGCACAATTCGTGGACGTGGGAAAATTGATCCCATTCTGGTGCCGGA                                                                                                                                                            >  2:1666461/1‑150
      GATTTCGCCAATGTGCTGACGCAGGTTGATACCCTGTTGATGCTGGAAGTGTATCCGGCTGGCGAAGCGCCAATTCCGGGAGCGGACAGCCGTTCGCTGTGTCGCACAATTCGTGGACGTGGGAAAATTGATCCCATTCTGGTGCCGGAT                                                                                                                                                           >  1:1050072/1‑150
      GATTTCGCCAATGTGCTGACGCAGGTTGATACCCTGTTGATGCTGGAAGTGTATCCGGCTGGCGAAGCGCCAATTCCGGGAGCGGACAGCCGTTCGCTGTGTCGCACAATTCGTGGACGTGGGAAAATTGATCCCATTCTGGTGCCGGAT                                                                                                                                                           >  1:1113524/1‑150
               AATGTGCTGACGCAGGTTGATACCCTGTTGATGCTGGAAGTGTATCCGGCTGGCGAAGCGCCAATTCCGGGAGCGGACAGCCGTTCGCTGTGTCGCACAATTCGTGGACGTGGGAAAATTGATCCCATTCTGGTGCCGGATCCGGCACCA                                                                                                                                                  >  1:4581332/1‑150
                              GTTGATACCCTGTTGATGCTGGAAGTGTATCCGGCTGGCGAAGCGCCAATTCCGGGAGCGGACAGCCGTTCGCTGTGTCGCACAATTCGTGGACGTGGGAAAATTGATCCCATTCTGGTGCCGGATCCGGCGCCAGAATGGGATCAATTT                                                                                                                                   <  2:1835248/150‑1
                               TTGATACCCTGTTGATGCTGGAAGTGTATCCGGCTGGCGAAGCGCCAATTCCGGGAGCGGACAGCCGTTCGCTGTGTCGCACAATTCGTGGACGTGGGAAAATTGATCCCATTCTGGTGCCGGATCCGGCACCAGAATGGGATCAATTTT                                                                                                                                  <  2:4581332/150‑1
                                                         TATCCGGCTGGCGAAGCGCCAATTCCGGGAGCGGACAGCCGTTCGCTGTGTCGCACAATTCGTGGACGTGGGAAAATTGATCCCATTCTGGTGCCGGATCCGGCACCAGAATGGGATCAATTTTCCCACG                                                                                                                            >  1:2872667/1‑130
                                                                               TTCCGGGAGCGGACAGCCGTTCGCTGTGTCGCACAATTCGTGGACGTGGGAAAATTGATCCCATTCTGGTGCCGGATCCGGCACCAGAATGGGATCAATTTTCCCACGTCCACGAATTGTGCGACACAGCGAACGGCTGTCCGCTCCCGG                                                                                  <  2:795123/150‑1
                                                                                                       TGTGTCGCACATTTCGTGAACGTGGGAAAATTGATCCCATTCTGGGGCGGGATCCGGCGCCAGAGTGGGATCATTTTCCCCACGTCCACGAATTGT‑‑GACACAGCGAACGGTTGTCCGCTCCCGGAATTGGCGCTTCGCCAGCCGGATACA                                                        <  2:4238962/150‑1
                                                                                                                           CGTGGGAAAATTGATCCCATTCTGGTGCCGGATCCGGCACCAGAATGGGATCAATTTTCCCACGTCCACGAATTGTGCGACACAGCGAACGGCTGTCCGCTCCCGGAATTGGCGCTTCGCCAGCCGGATA                                                          >  2:2872667/1‑130
                                                                                                                                                 ccGTGCCGGATCCGGCACCAGAATGGGATCAATTTTCCCACGTCCACGAATTGTGCGACACAGCGAACGGCTGGCCGCTCCCGGAATTGGCGGTTCGCCAGCCGGATACACTTCCAGCATCAACAGGGTATCAACCTGCGTCAGCACATT                <  2:358227/148‑1
                                                                                                                                                     GCCGGATCCGGCACCAGAATGGGATCAATTTTCCCACGTCCACGAATTGTGCGACACAGCGAACGGCTGTCCGCTCCCGGAATTGGCGCTTCGCCAGCCGGATACACTTCCAGCATCAACAGGGTATCAACCTGCGTCAGCACATTGGCG            <  1:2249184/150‑1
                                                                                                                                                        GGATCCGGCACCAGAATGGGATCAATTTTCCCACGTCCACGAATTGTGCGACACAGCGAACGGCTGTCCGCTCCCGGAATTGGCGCTTCGCCAGCCGGATACACTTCCAGCATCAACAGGGTATCAACCTGCGTCAGCACATTGGCGAAA         <  1:104843/150‑1
                                                                                                                                                           TCCGGCACCAGAATGGGATCAATTTTCCCACGTCCACGAATTGTGCGACACAGCGAACGGCTGTCCGCTCCCGGAATTGGCGCTTCGCCAGCCGGATACACTTCCAGCATCAACAGGGTATCAACCTGCGTCAGCACATTGGCGAAATCA      <  2:1180933/150‑1
                                                                                                                                                            CCGGCACCAGAATGGGATCAATTTTCCCACGTCCACGAATTGTGCGACACAGCGAACGGCTGTCCGCTCCCGGAATTGGCGCTTCGCCAGCCGGATACACTTCCAGCATCAACAGGGTATCAACCTGCGTCAGCACATTGGCGAAATCAc     <  2:4202220/150‑2
                                                                                                                                                            CCGGCACCAGAATGGGATCAATTTTCCCACGTCCACGAATTGTGCGACACAGCGAACGGCTGTCCGCTCCCGGAATTGGCGCTTCGCCAGCCGGATACACTTCCAGCATCAACAGGGTATCAACCTGCGTCAGCACATTGGCGAAATCAT     <  2:3185119/150‑1
                                                                                                                                                            CCGGCACCAGAATGGGATCAATTTTCCCACGTCCACGAATTGTGCGACACAGCGAACGGCTGTCCGCTCCCGGAATTGGCGCTTCGCCAGCCGGATACACTTCCAGCATCAACAGGGTATCAACCTGCGTCAGCACATTGGCGAAATCAT     <  2:3748212/150‑1
                                                                                                                                                             CGGCACCAGAATGGGATCAATTTTCCCACGTCCACGAATTGTGCGACACAGCGAACGGCTGTCCGCTCCCGGAATTGGCGCTTCGCCAGCCGGATACACTTCCAGCATCAACAGGGTATCAACCTGCGTCAGCACATTGGCGAAATCATC    <  1:1120634/150‑1
                                                                                                                                                               GCACCAGAATGGGATCAATTTTCCCACGTCCACGAATTGTGCGACACAGCGAACGGCTGTCCGCTCCCGGAATTGGCGCTTCGCCAGCCGGATACACTTCCAGCATCAACAGGGTATCAACCTGCGTCAGCACATTGGCGAAATCATCAT  <  2:4511734/150‑1
                                                                                                                                                               GCACCAGAATGGGATCAATTTTCCCACGTCCACGAATTGTGCGACACAGCGAACGGCTGTCCGCTCCCGGAATTGGCGCTTCGCCAGCCGGATACACTTCCAGCATCAACAGGGTATCAACCTGCGTCAGCACATTGGCGAAATCATCAT  <  1:234003/150‑1
                                                                                                                                                                                                                                                                                                                     
TATGATGATTTCGCCAATGTGCTGACGCAGGTTGATACCCTGTTGATGCTGGAAGTGTATCCGGCTGGCGAAGCGCCAATTCCGGGAGCGGACAGCCGTTCGCTGTGTCGCACAATTCGTGGACGTGGGAAAATTGATCCCATTCTGGTGCCGGATCCGGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  AP012306/96760‑96920
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gccggatccggcACCAGAATGGGATCAATTTTCCCACGTCCACGAATTGTGCGACACAGCGAACGGCTGTCCGCTCCCGGAATTGGCGCTTCGCCAGCCGGATACACTTCCAGCATCAACAGGGTATCAACCTGCGTCAGCACATTGGCGAAATCATCAT  <  AP012306/96908‑96761

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.