Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP012306341,3770TG31.2% 218.9 / 27.0 125G60G (GGT→GGG) ribDfused diaminohydroxyphosphoribosylaminopyrimidin e deaminase and 5‑amino‑6‑(5‑phosphoribosylamino) uracil reductase
Reads supporting (aligned to +/- strand):  ref base T (20/66);  new base G (39/0);  total (59/66)
Fisher's exact test for biased strand distribution p-value = 7.34e-18
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 194 total aligned reads displayed.

CTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTGCG‑ACCGCCT‑ATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCG  >  AP012306/341228‑341526
                                                                                                                                                      |                                                                                                                                                       
cTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGt                                                                                                                                                         >  1:1619795/1‑150 (MQ=255)
   aaGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGaa                                                                                                                                                      >  1:1475323/1‑150 (MQ=255)
        ggCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGGGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGgaaaaaa                                                                                                                                                 >  2:1636199/1‑148 (MQ=255)
         gcgcAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGcc                                                                                                                                                <  1:3317803/150‑1 (MQ=255)
         gcgcAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGcc                                                                                                                                                <  1:2535856/150‑1 (MQ=255)
         gcgcAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGcc                                                                                                                                                <  1:2210222/150‑1 (MQ=255)
         gcgcAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGGAAAAAcc                                                                                                                                                >  1:3919835/1‑150 (MQ=255)
         gcgcAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGGGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGGAAAAgac                                                                                                                                                >  2:1144092/1‑148 (MQ=255)
          cgcAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGAAAAAACCa                                                                                                                                               >  1:3283969/1‑150 (MQ=255)
           gcAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGgaaaaaacaa                                                                                                                                              >  2:508193/1‑145 (MQ=255)
             aaCGAGGACGTTTTACCCCGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTTCCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAg                                                                                                                                            <  2:4064505/150‑1 (MQ=255)
             aaCGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAg                                                                                                                                            <  1:2939409/150‑1 (MQ=255)
             aaCGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAg                                                                                                                                            <  1:1017026/150‑1 (MQ=255)
              aCGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAgg                                                                                                                                           <  1:3896343/150‑1 (MQ=255)
               cGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGGGGAAAAACCCAAAgt                                                                                                                                           >  1:4694693/1‑149 (MQ=255)
               cGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGt                                                                                                                                          <  2:2663217/150‑1 (MQ=255)
               cGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGt                                                                                                                                          <  1:1815188/150‑1 (MQ=255)
                 aGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTGc                                                                                                                                        <  1:128435/150‑1 (MQ=255)
                  ggACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTGCg                                                                                                                                       <  1:3191769/150‑1 (MQ=255)
                   gACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTGCG‑a                                                                                                                                     <  1:2418113/150‑1 (MQ=255)
                    aCGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTGCG‑Ac                                                                                                                                    <  1:1581539/150‑1 (MQ=255)
                    aCGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTGCG‑Ac                                                                                                                                    <  1:2127509/150‑1 (MQ=255)
                     cGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTGCG‑Acc                                                                                                                                   <  1:2228341/150‑1 (MQ=255)
                      gTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGAAAAAGCCAAAAGTGCG‑ACCg                                                                                                                                  >  1:3082416/1‑150 (MQ=255)
                       ttttACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGGAAAAGCCCAAGGTGCG‑ACCGc                                                                                                                                 >  1:174390/1‑150 (MQ=255)
                       ttttACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGGGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGGAAAAAACAAAAGAGCG‑Gcccc                                                                                                                                 >  2:2403129/1‑148 (MQ=255)
                          tACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTGCG‑ACCGCCT‑a                                                                                                                             <  1:4142294/150‑1 (MQ=255)
                            ccACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAAGCTAAGGTGCG‑ACCGcccttt                                                                                                                            >  1:432926/1‑146 (MQ=255)
                              aCGCATCCAACCCCGAATGTGGGGTGCGTCATTGTAAAAGATGGCGAAATTGTCGGTAAAGGTTCCCACCAACGTGCGGGTGAACCACATGCCGAAGTACCCGCGTTGTGTATGGG‑GGGTGAAAAAGCCAAAGGTGCG‑ACCGCCT‑ATGTc                                                                                                                         <  2:4441702/150‑1 (MQ=255)
                               cGCATCCCACCCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTGCG‑ACCGCCT‑ATGTca                                                                                                                        <  1:2654904/150‑1 (MQ=255)
                                   tCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGGAAAAACCAAAAGGGCG‑ACCGCCC‑ATGTTACACt                                                                                                                    >  1:4484862/1‑150 (MQ=255)
                                      caccccGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGGGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTAGGGG‑GGGTAAAAAAGCCAAAGGTGCG‑ACCGCCT‑ATGTCACACTCGa                                                                                                                 <  2:2915049/147‑1 (MQ=255)
                                      cAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGAAAAAACCAAAAGGGCG‑ACCGCCC‑ATGTCACACTcca                                                                                                                 >  1:1266609/1‑148 (MQ=255)
                                       aaCCCGAATGTGGGGTGCGTCATTGTAAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACCCGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTGCG‑ACCGCCT‑ATGTCACACTCGaa                                                                                                                <  2:4400757/150‑1 (MQ=255)
                                        aCCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAACCAAAGGGGCG‑ACCGCCC‑CTGTCACACTCGCAc                                                                                                               >  1:4038496/1‑150 (MQ=255)
                                         cccGAATGTGGGGTGCGTCATTGTAAAAGATGGAAAATTTGGGGGTGAGGGTTACCACAAGCGGGGGGGTAACCCACATGCGGAATTACACGGGTTGCGTAGGGG‑GGGTAAAAAACCAAAAGGTGCG‑ACCGCCT‑ATGTCACACTCGAAcc                                                                                                              <  2:3530566/150‑1 (MQ=255)
                                             aaTGTGGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGACCCACAGGCCGAAGTACACGCGTTGCGTATGGC‑GGGTAAAAAAGCCAAAGGTGCG‑ACCGCCT‑ATGTCACACTCGAACCCTGt                                                                                                          <  2:3146531/150‑1 (MQ=255)
                                              aTGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGAAAAAACCAAAAGGGCG‑ACCGCCT‑CTGTCACACTCGAACCCCGTa                                                                                                         >  1:900373/1‑150 (MQ=255)
                                                    ggggCGTCATTGTAAAAGAGGGCGAAATTGCGGGTGAGGGTTCCCACCAAGGTGCGGGTGAACCACAGGCCGAAGTACACGGGTTGCGTCTGGG‑GGGTGAAAAACCCAAGGGTGCG‑ACCGCCT‑ATGTCACACTCGAACCCTGTAGCcatc                                                                                                   <  2:1890741/147‑1 (MQ=255)
                                                      tGCTTCATTGAAAAAGAGGGGAAAATTGCCGGTGAAGGTTACCCCCAGGTGGCGTGTAAACCCGGTCCCGAACAACACGTTTGGCTTA‑GGC‑GGGTTGAAAAGCCAAAGGGGGGAACCGCCT‑ATGTCACACTCGAACCCTGTAGCcatcat                                                                                                 <  2:2002988/150‑1 (MQ=255)
                                                      tGCGTCATTGTAAAAGATGGCAAAATTGTCGGTGAAGTTTCCCCCCAACGTGGGGGTGACCCACATGCCGAAGTACACGCGTTGCGTATGGG‑GGGTGAAAAAGCCAAGGGTGCG‑ACCGCCT‑ATGTCACACTCGAACCCTGTAGCcatcat                                                                                                 <  2:2698685/150‑1 (MQ=255)
                                                       gctttaTTGTAAAAGATGGCAAAATTGTCGGTGAAGTTTACCCCCAACGTGCGGGTGAACAAAATGCCGAAGTACACGCGTTGCGTATGGC‑GGTTAAAAAACCAAAGGGTGCG‑ACCGCCT‑ATGTCACACTCGAACCCTGTAGCCATCATg                                                                                                <  2:1844057/145‑1 (MQ=255)
                                                        cGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGAAAAAACCAAAAGTGCG‑ACCGCCT‑ATGTCACACTCGAACCCCGTAGCCATCATgg                                                                                               >  1:1651580/1‑150 (MQ=255)
                                                         gTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGAAAAAGCCAAAGGGGCG‑ACCGCCC‑ATGTCACACTCGAACCCTGTAGCCATCATGGt                                                                                              >  1:1363430/1‑150 (MQ=255)
                                                          tCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTGCG‑ACCGCCT‑ATGTCACACTCGAACCCTGTAGCCATCATGGTc                                                                                             <  1:61799/150‑1 (MQ=255)
                                                          tCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTAAAAAAGCCAAAGGTGCG‑ACCGCCT‑ATGTCACACTCGAACCCTGTAGCCATCATGGTc                                                                                             <  1:768847/150‑1 (MQ=255)
                                                           cATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGAGAAAACCAAAAGTGCG‑GCCGCCT‑GTGTTACCCCCCAACCCCTTTGAcatcattggcg                                                                                            >  2:3398488/1‑145 (MQ=255)
                                                            aTTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGAAAAAGCCAAAGGTGCG‑ACCGCCC‑ATGTCCCACTCGAACCCTGTAGCCATCATTGTCGt                                                                                           >  1:3150127/1‑150 (MQ=255)
                                                             ttGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGAAAAAACCAAAAGGGCG‑ACCGCCC‑ATGTCACACTCGAACCCCGTAGCCATCATGGTCGTa                                                                                          >  2:2723422/1‑150 (MQ=255)
                                                              tGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGAAAAAACCCAAAGTGCG‑ACCGCCC‑ATTTCACACCCCAACCCCGTAGCCATCATGGTCGTAc                                                                                         >  2:3904006/1‑150 (MQ=255)
                                                                gaaaaGATGGCGAATTTGTCGGTGAAGGTTCCCCCCACCGGGCGGGGGAACCACAGCCCGAAGTCCCCGTGTGGCTTATGGC‑GGGGAAAAAACCCAAGGGGGCG‑ACCGCCT‑ATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGc                                                                                       <  2:828910/148‑1 (MQ=255)
                                                                       tgcgtaaaTGGTGGGGGAAGGTTACCCCCAGCGGGGGGGGAAACCACATGCCGAAGTACGCGGGTTGCGTATGGC‑GGGAGAAAAACCAAAAGGTGGG‑ACCGCCT‑ATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTg                                                                                <  2:1407654/145‑1 (MQ=255)
                                                                       tGGCGAAATTGTCGGTGAAGGTTCCCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAGGGTGCG‑ACCGCCT‑ATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTg                                                                                <  1:1948083/150‑1 (MQ=255)
                                                                       tGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGGAAAAAACAAAAGTGCG‑ACCGCCC‑ATGTCACACTCGAACCCCGTAGCCATCATGGTCGTACGCccccccg                                                                                >  2:3023206/1‑147 (MQ=255)
                                                                         gCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGAAAAAAGCAAAGGTGGG‑AACCCCC‑ATTTCACCCTCCCACCCCGTCGCCACCATCGTCGGACCCCACCCTGcg                                                                              >  2:4598197/1‑149 (MQ=255)
                                                                         gCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGAAAAAAACCAAAGCGGG‑ACCGCCC‑CTGTCACACACCAACCCTGTAACCCTTCTTGGCGTACGCccccccgcc                                                                              >  2:1089573/1‑145 (MQ=255)
                                                                          cGAAATTGTCGGTGAAGGTTACCACAAACGGGCGGGTGAACCACATGCCGAAGTACACGCGTTGGGTAGGGC‑GGGTGAAAAAGCCAAAGGTGCG‑ACCGCCT‑ATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCtg                                                                             <  2:4290523/150‑1 (MQ=255)
                                                                           gAAATTGTCGGTGAAGGTTACCACCACCGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTGCG‑ACCGCCT‑ATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCtgt                                                                            <  1:3292217/150‑1 (MQ=255)
                                                                           gAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGAAAAAGCCAAAAGGGCG‑ACCGCCC‑ATGTCACACTCCAACCCCGTAGCCATCATGGGCGTACGCCCCCCTGCtgt                                                                            >  2:1567794/1‑150 (MQ=255)
                                                                            aatttGTCGGTGAGGTTTCCCACAAACGGGGGGGTGAACCCCATCCGGAAGTACCGGCGTTGGGTTTGGC‑GGTTGAAAAACCCAAAGGTGCG‑ACCGCCT‑ATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCtgtg                                                                           <  2:2171403/147‑1 (MQ=255)
                                                                              tttGTGGGTGATGTTTACAACAAGGGTGCGGGCGAACCACAGGCGGAAGTACACGCGTTGCGTAGGGG‑GGGTGAAAAAGCAAAAGGTGCA‑ACCGCCT‑ATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGAc                                                                         <  2:1272628/149‑1 (MQ=255)
                                                                               ttGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTGCG‑ACCGCCT‑ATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACg                                                                        <  1:2479821/150‑1 (MQ=255)
                                                                                  tCGGTGAAGTTTACCACCAAGGTGGGGGTAACCCACATGCCGAAGTACCCGCGTTGCGTAGGGG‑GGGTAAAAAAGCAAAAGGTGCG‑ACCGCCT‑ATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCAc                                                                     <  2:3491429/150‑1 (MQ=255)
                                                                                   cGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGAAAAAAACAAAGGGGCG‑ACCGCCC‑ATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCCTGCTGTGACGCACt                                                                    >  2:64873/1‑150 (MQ=255)
                                                                                    ggTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGAAAAAACCAAAAGGGCG‑ACCGCCC‑ATGTCACACCCCAACCCCGTAGCCATCATGGGCGGACGCCCCCCCGCCGTGGCGCACTc                                                                   >  2:897056/1‑150 (MQ=255)
                                                                                          ggTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGAAAAAGCCAAAGGGGCG‑ACCGCCC‑CTGTCACACCCGAACCCTGTAGCCATCATGGGCGTACGCCACCGTGCTGTGACGCACTCATcgcc                                                             >  2:8869/1‑150 (MQ=255)
                                                                                           gTTAACACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTGCG‑ACCGCCT‑ATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATcgccg                                                            >  2:55494/1‑150 (MQ=255)
                                                                                               ccaccaGGGTGGGGGTGAACCACATGCCGAAGTACACGCGTTGCGTAGGGC‑GGGTGAAAAAGCAAAAGGTGCG‑ACCGCCT‑ATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTgg                                                        <  2:1919543/150‑1 (MQ=255)
                                                                                                cacaaACGGGGGGGTGAACCACATGCGGAAGTCCCGGCGTGGTGTTTGGG‑GGGTGAAAAAGCAAAGGGTGCG‑ACCGCCT‑ATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGc                                                       <  2:4173710/150‑1 (MQ=255)
                                                                                                       gTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGGGGAAAAAACCAAA‑GTGGG‑ACCGCCC‑ATGGCACACTCCAACCCTGTAACCCTCATGGTTGGACCCCCCCGTGCGGTGGCGCACTCATCGCCCCCGGCGGAgcgc                                                >  2:48674/1‑150 (MQ=255)
                                                                                                         gCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGAAAAAACCAAAAGGGCG‑ACCGCCC‑ATGTCACACTCGAACCCCGTAGCCATCATGGGCGGACGCCCCCCTGCTGTGACGCACTCCTCGCCCCCGGCGTGgcgcgc                                              >  2:3361857/1‑150 (MQ=255)
                                                                                                          cGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGAAAAAACCAAAAGGGGG‑ACCGCCT‑ATGTCACACTCGCACCCCGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGcgcgcg                                             >  2:743697/1‑150 (MQ=255)
                                                                                                               gAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGAAAAAACCAAAAGGGCG‑GCCGCCC‑ATGTCCCACTCGAACCCCTTAGCCATCATGGGCGTACGCCACCGTGCCGTGACGCACTCATCGCCCCTGGCGTAGCGCGCGTgggt                                        >  2:1939202/1‑148 (MQ=255)
                                                                                                                   cacaTGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGGAAAAGCCAAAGGGGCG‑ACCCCCT‑ATGGCCCCCTCGGACCCCGGAGCCATCAAGGGCGTACGCCCCCGGGCCGGGACGCACTCCTCCCCCCGGGCGGAGCGCGCGCGGTTGccc                                    >  2:1732473/1‑149 (MQ=255)
                                                                                                                   cacaTGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGGAAAAAGAAAAAGTGAG‑CCCGACTCATG‑CCCACTCTAAACCTGTCCCCTCCCTGGTCCTACGCCACCCGGCTGGGGCGCGCTCATCCACCCCGGCGTCGCTCGCGAGGGTGCCt                                    >  2:2042388/1‑150 (MQ=255)
                                                                                                                      aTGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAACCAAAGGGGCG‑ACCCCCC‑ATTTCACACCCGAAACCCGGAGGCATCTTTGGCGGGCGCCCCCGTCGTTTTAGGCGCTCCTCGCCGCTGGCGGAGCCCGCGTGGGTGCGTCGa                                 >  2:152728/1‑150 (MQ=255)
                                                                                                                           gAAGTACACGCGTTGCGTATGGC‑GGGTAAAAAAGCAAAAGGTGCG‑ACCGCCT‑ATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCaa                            <  1:2890220/150‑1 (MQ=255)
                                                                                                                             aGTACACGCGTTGCGTATGGC‑GGGGGAAAAAAACAAAAGGGCG‑ACCGCCC‑ATGTCACACTCGAACCCTGTAGCCATCATGGGCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGGAGCGCGCGTGGTTGCCTCGATGCAAGa                          >  2:3830318/1‑150 (MQ=255)
                                                                                                                              gTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTGCG‑ACCGCCT‑ATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGAt                         <  1:4216021/150‑1 (MQ=255)
                                                                                                                              gTACACGCGTTGCGTAGGGG‑GGGTAAAAAAGCCAAAGGTGCG‑ACCGCCT‑ATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGAt                         <  2:4226466/150‑1 (MQ=255)
                                                                                                                               taccggcgTTGCGTATGGC‑GGGTGAAAAGGCAAAAGGTGCG‑ACCGCCT‑ATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATc                        <  1:237013/145‑1 (MQ=255)
                                                                                                                               tACACGCGTTGCGTATGGC‑GGGTGAAAAAACCAAAAGGGCC‑ACCCCCC‑CTGTCACACCCGAAACCCGTAGCCCTCAAGGGTGGACCCCCCCGGGCTGTGACGCACTCATCACCCCTCGCGCAGCGCGCGCGGGTGCCCCGATGCCAGATc                        >  2:1247009/1‑150 (MQ=255)
                                                                                                                               tACACGCGTGGCGTAGGGC‑GGGTGAAAAAGCAAAAGGTGCG‑ACCGCCT‑ATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATc                        <  1:1029648/150‑1 (MQ=255)
                                                                                                                                 cacGCGTTGCGTATGGC‑GGGTGAAAAGGCAAAAGGTGCG‑ACCGCCT‑ATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCt                      <  1:2748838/150‑1 (MQ=255)
                                                                                                                                  acGCGTTGCGTATGGC‑GGGTGAAAAAACCAAAAGGGCG‑ACCGCCC‑ATGTCACACTCCAACCCCGTAGCCATCATGGTCGTACGCCCCCGCGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTa                     >  2:1439857/1‑150 (MQ=255)
                                                                                                                                  acGCGTTGCGTAGGGC‑GGGTAAAAAAGCAAAAGGTGCG‑ACCGCCT‑ATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTa                     <  1:458129/150‑1 (MQ=255)
                                                                                                                                   cgcgTTGCGTATGGG‑GGGTAAAAAAGCCAAGGGTGCG‑ACCGCCT‑ATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTaa                    <  2:322890/150‑1 (MQ=255)
                                                                                                                                    gggTTGCGTAGGGC‑GGGTAAAAAAGCAAAGGGTGCG‑CCCGCCT‑ATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAAc                   <  2:2342768/148‑1 (MQ=255)
                                                                                                                                    gcgTTGCGTATGGG‑GGGTGAAAAAGCAAAGGGTGCG‑ACCGCCT‑ATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAAc                   <  1:2250770/150‑1 (MQ=255)
                                                                                                                                     cgTTGCGTATGGC‑GGGTGAAAAAGGCAAAAGGGCG‑GCCCCCC‑ATGTCACACTCGGACCCTCTAGCCCTCATGGTCCGACGCCCCCGGGCTGTGGCGCACTCATCGCCGCCGGCGCAACGCGCGTGGGTGCCTCCCTGCAAAAACCTAAcc                  >  2:3884875/1‑150 (MQ=255)
                                                                                                                                      gTTGCGTATGGC‑GGGTGAAAAAACCAAAAGTGCG‑ACCGCCT‑ATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAAccc                 >  1:3664421/1‑150 (MQ=255)
                                                                                                                                          cGTATGGC‑GGGTGAAAAAGCCAAAGGTGCG‑ACCGCCT‑ATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAg             <  1:2601759/150‑1 (MQ=255)
                                                                                                                                          cGTATGGC‑GGGTGAAAAAACCAAAAGGGCG‑ACCGCCT‑ATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGGAGCGCGCGTGGTTGCATCGATGCAAGATCCTAACCCCCAg             >  2:4203860/1‑150 (MQ=255)
                                                                                                                                           gTATGGC‑GGGGGAAAAAGGCAAAGGGGCG‑ACCGCCT‑ATGTCACACTCGAACCCTGGAGCCATCATGGGCGGACGCCACCGGGCTGTGACGCACTCATCGCCGCTGGCGTAGGGCGCGTGGGTGCCTCGATGCAAGAACCTAACCCGCAgg            >  2:3988789/1‑150 (MQ=255)
                                                                                                                                              tGGC‑GGGGGAAAAAGCCAAAGGAGCG‑CCCGCCT‑TTGTCACACTCGAACCCTGTAGCCATCATGGTCGCACGCCACCGCGCTGTGACGCACTCATCCCCGCTGGCGTAGCGCGCCTGGTTGCCTCGATGCAAGACCCCAACCCGCAGGGCg         >  2:668728/1‑150 (MQ=255)
                                                                                                                                                 gggggTGAAAAAGCCAAGGGTGCG‑ACCGCCT‑ATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCt       <  2:1404099/148‑1 (MQ=255)
                                                                                                                                                    ggTGAAAAAGCC‑AAGGTGCG‑ACCGCCT‑ATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGc   >  1:4369743/1‑150 (MQ=255)
                                                                                                                                                     gTGAAAAAGCCAAAGGTGCG‑ACCGCCT‑ATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGc   >  2:4548688/1‑150 (MQ=255)
                                                                                                                                                     gTGAAAAAGCCAAAGGTGCG‑ACCGCCT‑ATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGc   >  2:2772997/1‑150 (MQ=255)
                                                                                                                                                      tGAAAAAGCCAAAGGTGCG‑ACCGCCT‑ATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCg  >  1:3503592/1‑150 (MQ=255)
                                                                                                                                                      |                                                                                                                                                       
CTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTGCG‑ACCGCCT‑ATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCG  >  AP012306/341228‑341526

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑