Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP012306800,6750AC33.0% 529.9 / 56.7 274T192P (ACC→CCC) lolAchaperone for lipoproteins
Reads supporting (aligned to +/- strand):  ref base A (144/39);  new base C (0/90);  total (144/130)
Fisher's exact test for biased strand distribution p-value = 2.20e-41
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 349 total aligned reads displayed.

GCAATGGCAATCTGAAGCAGTTCACCATTAACGTGGGACGTGATGGCACAATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGC‑AGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCAGAAAATTTAGCACAGTATATCGGCCAGCAACA  >  AP012306/800526‑800824
                                                                                                                                                      |                                                                                                                                                     
   aTGGCAATCTGAAGCAGTTCACCATTAACGTGGGACGTGATGGCACAATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGC‑AGCGAAATTTACCTTCAccc                                                                                                                                                    <  2:1415856/150‑1 (MQ=255)
    tGGCAATCTGAAGCAGTTCACCATTAACGTGGGACGTGATGGCACAATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGC‑AGCGAAATTTACCTTCAcccc                                                                                                                                                   <  2:4596232/150‑1 (MQ=255)
        aaTCTGAAGCAGTTCACCATTAACGTGGGACGTGATGGCACAATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGC‑AGCGAAATTTACCTTCACCccgccg                                                                                                                                               >  2:3557846/1‑150 (MQ=255)
          tCTGAAGCAGTTCACCATTAACGTGGGACGTGATGGCACAATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGC‑AGCGAAATTTACCTTCCCCCCGCCGCa                                                                                                                                             >  2:2162010/1‑150 (MQ=255)
            ggAAGCAGTTCCCCTTAAACGGGGGCCGTGAGGGCAAAACCCATCAGTTTCGCGGGGTGGAGCAGGACGACCAGCGCAGCAGTTATCAACTGAAACCCCAGCAAAAGGGGTGTGGGGTTGC‑GGCAAAATTTACCTTCCCCCCGCCGCAAg                                                                                                                                           <  2:837892/149‑1 (MQ=255)
                    ttCACCATTAACGTGGGACGTGATGGCACAATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGC‑AGCGAAATTTACCTTCCCCCCGCCGCAAGGCGTCACg                                                                                                                                   <  1:392216/150‑1 (MQ=255)
                    ttCACCATTAACGTGGGACGTGATGGCACAATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGC‑AGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACg                                                                                                                                   >  1:4620623/1‑150 (MQ=255)
                      cACCATTAACGTGGGACGTGATGGCACAATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGC‑AGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGt                                                                                                                                 >  2:556484/1‑150 (MQ=255)
                             aaCGTGGGACGTGATGGCACAATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGC‑AGCGAAATTTCCCTTCCCCCCGCCGCAAGGCGTCACGGTAgatgat                                                                                                                          <  1:451549/150‑1 (MQ=255)
                             aaCGTGGGACGTGATGGCACAATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGC‑AGCGAAATTTACCTTCCCCCCGCCGCAAGGCGTCACGGTAgatgat                                                                                                                          <  1:2902569/150‑1 (MQ=255)
                             aaCGTGGGACGTGATGGCACAATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGC‑AGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAgatgat                                                                                                                          >  1:4479296/1‑150 (MQ=255)
                             aaCGTGGGACGTGATGGCACAATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGGGGATGC‑AGCGAAATTTCCCTTCCCCCCGCCGCAAGGCGTCACGGTAgatgat                                                                                                                          <  2:1620517/150‑1 (MQ=255)
                              aggggggACGTGTTGCCACAATCCAAGTTTGTCGCGGGGTGGAGCAGAACGATCAGCGAGGCACTGACCAAATTAAAGCCCAGAAAAGGGGGGTTGTGGAGGC‑AGGGAATTTTCCCTTCCCCCCGCCGCAAGGCGTCACGGTAGATGATc                                                                                                                         <  2:2345054/146‑1 (MQ=255)
                              aCGTGGGACGTGATGGCACAATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCATTTATCACCTAAAACCCCAGCAAAATGGGGCTGTGGATGC‑AGCGAAATTTCCCTTCCCCCCGCCGCAAGGCGTCACGGTAGATGATc                                                                                                                         <  2:2883683/150‑1 (MQ=255)
                              aCGTGGGACGTGATGGCACAATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGC‑AGCGAAATTTACCTTCCCCCCGCCGCAAGGCGTCACGGTAGATGATc                                                                                                                         <  1:192442/150‑1 (MQ=255)
                                ggggaaCGTGATGGCACAATCCATCAGTTTAGCGCGGTGGAGCAGGACAATCAGCGCAGCATTTATCAACTAAAACCCCAGAAAAATGGGGTTGTGGATGA‑AGCAAAATTTCCCTTCCCCCCGCCGCAAGGCGTCACGGTAGATGATCaa                                                                                                                       <  2:4657334/145‑1 (MQ=255)
                                gTGGGACGTGATGGCACAATCCATCAGTTTAGCGGGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAAGCCAAGCAAAAGGGGGTTGTGGATGC‑AGAGAAATTTCCCTTCCCCCCGCCGCAAGGCGTCACGGTAGATGATCaa                                                                                                                       <  2:3982037/150‑1 (MQ=255)
                                    gACGTGATGGCAGAATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGC‑AGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTa                                                                                                                   >  2:4719279/1‑150 (MQ=255)
                                      cGTGATGGCACAATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGC‑AGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAg                                                                                                                 >  2:3324333/1‑150 (MQ=255)
                                         gATGGCACAATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGC‑AGCGAAATTTACCTTCCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTag                                                                                                              <  1:1566305/150‑1 (MQ=255)
                                          aTGGCACAATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGC‑AGCGAAATTTACCTTCCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTaga                                                                                                             <  2:3403043/150‑1 (MQ=255)
                                             gCACAATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGC‑AGCGAAATTTACCTTCCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGc                                                                                                          <  1:206289/150‑1 (MQ=255)
                                             gCACAATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGC‑AGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGc                                                                                                          >  1:130390/1‑150 (MQ=255)
                                               acaATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGC‑AGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCAc                                                                                                        >  2:2584523/1‑150 (MQ=255)
                                                   tCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGC‑AGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCtga                                                                                                    >  1:3715054/1‑150 (MQ=255)
                                                    ccATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCATCAAAATGGGGCTGTGGATGC‑AGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCtgag                                                                                                   >  1:2006914/1‑150 (MQ=255)
                                                    ccATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGC‑AGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCtgag                                                                                                   >  2:1823335/1‑150 (MQ=255)
                                                        cAGTTAAGCGGGGTGGAGCAGGACGACCAGCGCACCAGTTATCAACTGAAATCCCAGAAAAAGGGGGCTGTGGATGA‑AGCGAAATTTACCTTCCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCtgagtgag                                                                                               <  2:3796722/150‑1 (MQ=255)
                                                           tttAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGC‑AGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCaa                                                                                            >  2:895233/1‑150 (MQ=255)
                                                           tttAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGC‑AGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCaa                                                                                            >  1:260892/1‑150 (MQ=255)
                                                            ttAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAAGGGGGCTGGGGATGC‑AGCGAAATTTCCCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAAt                                                                                           <  2:3277634/150‑1 (MQ=255)
                                                                cgcgGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTAAAATCCCAGCAAAAGGGGGCTGTGGAGGC‑AGCGAATTTTACCTCCCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGt                                                                                       <  2:2378854/150‑1 (MQ=255)
                                                                cgcgGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTAAAACCCCAGCAAAATGGGGCTGTGGATGC‑AGCGAAATTTACCTCCCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGt                                                                                       <  2:2803749/150‑1 (MQ=255)
                                                                 gcgGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAACCCCAGCAAAATGGGGCTGTGGATGC‑AGCGAAATTTCCCTTCCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTc                                                                                      <  2:477444/150‑1 (MQ=255)
                                                                   ggTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGC‑AGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGc                                                                                    >  2:1226081/1‑150 (MQ=255)
                                                                     tGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGC‑AGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTc                                                                                  >  2:395157/1‑150 (MQ=255)
                                                                     tGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGC‑AGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTc                                                                                  >  1:2431850/1‑150 (MQ=255)
                                                                       gAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGC‑AGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGa                                                                                >  2:4548288/1‑150 (MQ=255)
                                                                         gCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGC‑AGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGAtt                                                                              <  1:700255/150‑1 (MQ=255)
                                                                          gaGGAAGCTCTGCGTAGCGGTTATCAACATAAATCCCACAAAAGTGGGGCTGGGGAGGC‑GGCGAAATTTGCCCCCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGAttt                                                                             <  2:4263913/149‑1 (MQ=255)
                                                                          cAGAACGACCAGCGCAGCAGTTATCAACTGAAACCCCAGCAAAATGGGGGTGTGGATGC‑AGCAAAATTTACCTCCCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGAttt                                                                             <  2:1774466/150‑1 (MQ=255)
                                                                            ggACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGAAAAAGGGGGCTGTGGATGC‑AGCGAAATTTACCTTCCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGAttttt                                                                           <  1:725260/150‑1 (MQ=255)
                                                                              aCGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGC‑AGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCg                                                                         >  2:2005671/1‑150 (MQ=255)
                                                                              aCGATCAGCGCAGCAGTTATCAACTGAAATCCCAGAAAAATGGGGCTGTGGATGC‑AGCGAAATTTCCCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCg                                                                         <  1:3060930/150‑1 (MQ=255)
                                                                               cGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGC‑AGCGAAATTTACCTTCCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCgg                                                                        <  2:3617022/150‑1 (MQ=255)
                                                                                  tCAGCGCAGCAGTTATCAACTGAAACCCCAGCAAAATGGGGCTGTGGATGC‑AGCGAAATTTACCTTCCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGata                                                                     <  1:4221158/150‑1 (MQ=255)
                                                                                     gcgcAGAATTTATCAACGGAAACCCCAACAAATTGGGCGTGT‑GATGCGAGTAAAATTTCCCTCGCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGataata                                                                  <  2:1912848/150‑1 (MQ=255)
                                                                                      cgcAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGC‑AGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATAc                                                                 >  2:2662468/1‑150 (MQ=255)
                                                                                       gcagcaGTTATCAACTAAAACCCCAGCAAAAGGGGGCTGTGGATGC‑AGCGAAATTTACCTTCCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACt                                                                <  2:3453840/150‑1 (MQ=255)
                                                                                         agcagTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGC‑AGCGAAATTTACCTTCCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACttt                                                              <  1:3750894/150‑1 (MQ=255)
                                                                                         agcagTTATAAAGTGAAATCCCAGCAAACGGGGGCTGTGGATGC‑AGCGAATTTTCCCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACttt                                                              <  2:1729005/150‑1 (MQ=255)
                                                                                          gcagTTATCAACTGAAACCCCAGAAAAATGGGGCTGTGGATGC‑AGCGAAATTTCCCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACtttt                                                             <  2:4202610/150‑1 (MQ=255)
                                                                                            ggTTATCATCTGAACCCCCGGAAAATGGGGGCTGGGGGTGC‑AGCGAAATTTCCCTTCCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCa                                                           <  2:2263871/149‑1 (MQ=255)
                                                                                            agTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGC‑AGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCa                                                           >  2:750584/1‑150 (MQ=255)
                                                                                            agTTATCAACTGAAATCCCAGAAAAATGGGGCTGTGGATGC‑AGCAAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCa                                                           <  1:2288096/150‑1 (MQ=255)
                                                                                            agTTATCAACTGAAATCCCAGAAAAAGGGGGCTGTGGATGC‑AGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCa                                                           <  1:2926207/150‑1 (MQ=255)
                                                                                             gTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGC‑AGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCaa                                                          >  1:1068510/1‑150 (MQ=255)
                                                                                              ttATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGC‑AGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAAc                                                         >  1:2220126/1‑150 (MQ=255)
                                                                                              ttATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGC‑AGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAAc                                                         >  2:4003718/1‑150 (MQ=255)
                                                                                               tATCAAGTGAAATCCCAGCAAATGGGGGCTGGGGATGC‑CGCAAAATTTCCCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAAcc                                                        <  2:231598/150‑1 (MQ=255)
                                                                                                aTCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGC‑AGCGAAATTTCCCTCCCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACct                                                       <  1:556682/150‑1 (MQ=255)
                                                                                                aTCAACTAAAATCCCAGCAAAATGGGGCTGTGGATGC‑AGCGAAATTTCCCTCCCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACct                                                       <  2:664649/150‑1 (MQ=255)
                                                                                                 tCACCTGAAATCCCAGCAAAATGGGGCTGTGGATGC‑AGCGAAATTTCCCTTCCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACctc                                                      <  1:4157041/150‑1 (MQ=255)
                                                                                                  cAACTGAAATCCCAGCAAAATGGGGCTGTGGATGC‑AGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACctct                                                     <  1:1838352/150‑1 (MQ=255)
                                                                                                     cTGAAATCCCAGCAAAATGGGGCTGTGGATGC‑GGCGAAATTTACCTCCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGc                                                  <  1:914338/150‑1 (MQ=255)
                                                                                                      tGAAATCCCAGCAAAATGGGGCTGTGGATGC‑AGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGcc                                                 >  2:2993659/1‑150 (MQ=255)
                                                                                                      tGAAATCCCAGAAAAATGGGGCTGTGGATGC‑AGCAAAATTTACCTTCCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGcc                                                 <  2:2446277/150‑1 (MQ=255)
                                                                                                      tGAAACCCCAGCAAAATGGGGCTGTGGATGC‑AGCGAAATTTACCTTCCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGcc                                                 <  2:2074572/150‑1 (MQ=255)
                                                                                                         aaTCCCAGCAAAATGGGGCTGTGGATGC‑AGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCcgcg                                              >  2:4297836/1‑150 (MQ=255)
                                                                                                               aGCAAAATGGGGTTGTGGATGC‑AGCGAAATTTACCTCCCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATg                                        <  1:865094/150‑1 (MQ=255)
                                                                                                               aGCAAAATGGGGCTGTGGATGC‑AGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATg                                        >  2:4332891/1‑150 (MQ=255)
                                                                                                                 cAAAATGGGGCTGGGGATGC‑AGCGAAATTTCCCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCg                                      <  1:1009884/150‑1 (MQ=255)
                                                                                                                 aaaaaGGGGGCTGTGGATGC‑AGCAAAATTTACCTTCCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCg                                      <  1:1092087/149‑1 (MQ=255)
                                                                                                                  aaaaTGGGGCTGTGGATGC‑AGCGAAATTTCCCTTCCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCgg                                     <  1:4710990/150‑1 (MQ=255)
                                                                                                                  aaaaTGGGGCTGTGGATGC‑AGCGAAATTTCCCTCCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCgg                                     <  1:4387881/150‑1 (MQ=255)
                                                                                                                  aaaaTGGGGCTGTGGATGC‑AGCGAAATTTACCTTCCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCgg                                     <  1:2860139/150‑1 (MQ=255)
                                                                                                                    aaTGGGGCTGTGGATGC‑AGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGcc                                   >  2:2122464/1‑150 (MQ=255)
                                                                                                                     aTGGGGCTGTGGATGC‑AGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCa                                  >  1:3367723/1‑150 (MQ=255)
                                                                                                                        gggCTGTGGATGC‑AGGAAAATTTACCTTCCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCAGaa                               <  1:1712548/150‑1 (MQ=255)
                                                                                                                          gCTGTGGATGC‑AGCAAAATTTACCTCCCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCAGaaaa                             <  2:599330/150‑1 (MQ=255)
                                                                                                                           cTGTGGATGC‑AGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCAGAAAAt                            >  2:4235094/1‑150 (MQ=255)
                                                                                                                             gtgGATGC‑AGCGAAATTTACCTTCCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCAGAAAAttt                          <  1:3230370/150‑1 (MQ=255)
                                                                                                                             ggggATGC‑AGCGAAATTTACCTTCCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCAGAAAAttt                          <  2:3102656/148‑1 (MQ=255)
                                                                                                                                ggtGC‑AGCGAATTTTCCGTTACCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCAGAAAATTTAGc                       <  1:2705992/148‑1 (MQ=255)
                                                                                                                                gATGC‑AGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCAGAAAATTTAGc                       <  1:16652/150‑1 (MQ=255)
                                                                                                                                gATGC‑AGCAAAATTTACCTTCCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCAGAAAATTTAGc                       <  2:3745659/150‑1 (MQ=255)
                                                                                                                                agtGC‑GGCGAGTTTTGCCTGCGCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCAGAAAATTTAGc                       <  1:3468676/148‑1 (MQ=255)
                                                                                                                                    c‑AGCGAAATTTACCTCCCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCAGAAAATTTAGCACAg                   <  1:1438280/150‑1 (MQ=255)
                                                                                                                                      aGCAAAATTTCCCTCCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCAGAAAATTTAGCACAGt                  <  2:370167/150‑1 (MQ=255)
                                                                                                                                         gAAATTTACCTTCACCCCGCCGCAAGGCTTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCAGAAAATTTAGCACAGtata               >  1:3959545/1‑150 (MQ=255)
                                                                                                                                           aaTTTCCCTTCCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCAGAAAATTTAGCACAGTATATc             <  1:1551808/150‑1 (MQ=255)
                                                                                                                                             tttACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCAGAAAATTTAGCACAGTATATCgg           >  2:1555270/1‑150 (MQ=255)
                                                                                                                                              ttACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCAGAAAATTTAGCACAGTATATCGGc          >  1:3188579/1‑150 (MQ=255)
                                                                                                                                                 ccTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCAGAAAATTTAGCACAGTATATCGGCCAg       >  2:4166331/1‑150 (MQ=255)
                                                                                                                                                  cTTCCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCAGAAAATTTAGCACAGTATATCGGCCAGc      <  2:1332156/150‑1 (MQ=255)
                                                                                                                                                  cTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCAGAAAATTTAGCACAGTATATCGGCCAGc      >  2:4458027/1‑150 (MQ=255)
                                                                                                                                                  cTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCAGAAAATTTAGCACAGTATATCGGCCAGc      >  2:2192388/1‑150 (MQ=255)
                                                                                                                                                   ttCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCAGAAAATTTAGCACAGTATATCGGCCAGCa     >  1:2387205/1‑150 (MQ=255)
                                                                                                                                                     cACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCAGAAAATTTAGCACAGTATATCGGCCAGCAAc   >  2:2484570/1‑150 (MQ=255)
                                                                                                                                                      aCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCAGAAAATTTAGCACAGTATATCGGCCAGCAACa  >  2:1483716/1‑150 (MQ=255)
                                                                                                                                                      |                                                                                                                                                     
GCAATGGCAATCTGAAGCAGTTCACCATTAACGTGGGACGTGATGGCACAATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGC‑AGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCAGAAAATTTAGCACAGTATATCGGCCAGCAACA  >  AP012306/800526‑800824

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑