Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123061,066,3230AC25.8% 623.2 / 25.9 245intergenic (‑92/+33)ychM/prsApredicted transporter/phosphoribosylpyrophosphate synthase
Reads supporting (aligned to +/- strand):  ref base A (158/23);  new base C (0/63);  total (158/87)
Fisher's exact test for biased strand distribution p-value = 2.42e-39
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 285 total aligned reads displayed.

GCAAGCGTCGATCAGAGCGCGGAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTG‑TCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGG‑ATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCACGTTCGGCAGTGATTTGATTTCATCGCT  >  AP012306/1066175‑1066472
                                                                                                                                                      |                                                                                                                                                     
gCAAGCGTCGATCAGAGCGCGGAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTG‑TCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGG‑ATATTACAGACAAAAAAAc                                                                                                                                                      >  1:3595897/1‑150 (MQ=255)
 cAAGCGTCGATCAGAGCGCGGAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTG‑TCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGG‑ATATTACAGACAAAAAAAcc                                                                                                                                                     <  2:2899306/150‑1 (MQ=255)
  aaGCGTCGATCAGAGCGCGGAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTG‑TCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGG‑ATATTACAGACAAAAAAAccc                                                                                                                                                    >  1:1292909/1‑150 (MQ=255)
  aaGCGTCGATCAGAGCGCGGAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTG‑TCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGG‑ATATTACAGACAAAAAAAccc                                                                                                                                                    <  1:446174/150‑1 (MQ=255)
   aGCGTCGATCAGAGCGCGGAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTG‑TCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGG‑ATATTACAGACAAAAAAACccg                                                                                                                                                   <  1:2762070/150‑1 (MQ=255)
      gTCGATCAGAGCGCGGAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTG‑TCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGG‑ATATTACAGACAAAAAAACccgccg                                                                                                                                                >  2:1754362/1‑150 (MQ=255)
         gATCAGAGCGCGGAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTG‑TCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGG‑ATATTACAGACAAAAAAACCCGCCGCAg                                                                                                                                             >  2:1662322/1‑150 (MQ=255)
            cAGAGCGCGGAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTG‑TCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGG‑ATATTACAGACAAAAAAACCCGCCGCAGCgg                                                                                                                                          >  2:3435951/1‑150 (MQ=255)
              gagCGCGGAAAGGCATCACATGTGAGGAAAATATTTTGTTCAAAATAATGTTTCACCCGTGAGCGAATCATCTG‑TCAACAAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGG‑ATATTACAAAAAAAAAACCCCGCCGCAGCGGGt                                                                                                                                        <  2:4480030/150‑1 (MQ=255)
              gagCGCGGAAAGGAATCACAGGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTG‑TAAACAAAAGGGCAGGGGAGGGAGGAAAGGGTAAAAAAAATGG‑ATATTACAAAAAAAAAACCCCGCCGCAGCGGGt                                                                                                                                        <  2:1294062/150‑1 (MQ=255)
                gcgcGGAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTG‑TCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGG‑ATATTACAGACAAAAAAACCCGCCGCAGCGGGTCt                                                                                                                                      >  1:2234594/1‑150 (MQ=255)
                 cgcgGAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTG‑TCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGG‑ATATTACAGACAAAAAAACCCGCCGCAGCGGGTCtt                                                                                                                                     >  2:1529285/1‑150 (MQ=255)
                           cATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTG‑TCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGG‑ATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGt                                                                                                                           >  2:2181410/1‑150 (MQ=255)
                           cATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTG‑TCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGG‑ATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGt                                                                                                                           >  1:429191/1‑150 (MQ=255)
                           cATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTG‑TCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGG‑ATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGt                                                                                                                           >  1:1450626/1‑150 (MQ=255)
                           cATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTG‑TCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGG‑ATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGt                                                                                                                           >  1:492823/1‑150 (MQ=255)
                           atccacaTGTGAGAAAAATTTTTTGTTCACAATATTGTTTCATCCGGGAGCGAATAATCTG‑TCAACAAAATGGCAGGTGAGGGAGGCATGGGTCATAAAAATGG‑ATTTTAAAAAAAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGt                                                                                                                           <  2:3594694/147‑1 (MQ=255)
                            aTCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTG‑TCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGG‑ATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGtt                                                                                                                          >  1:768376/1‑150 (MQ=255)
                               acaTGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTG‑TCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGG‑ATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGa                                                                                                                       >  2:320356/1‑150 (MQ=255)
                                  tgtgAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTG‑TCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGG‑ATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTa                                                                                                                    >  2:394368/1‑150 (MQ=255)
                                   gtgAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTG‑TCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGG‑ATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAg                                                                                                                   >  2:2118071/1‑150 (MQ=255)
                                       ggAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTG‑TCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGG‑ATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGtt                                                                                                               >  1:1121953/1‑150 (MQ=255)
                                       gaaaaaTTTTTTGTTCAAAATAATGTTTCACCGGTGCGCGCATCATCTG‑TAAACAAAATGGCAGGGGAGGGAGGCATGGGTCATACAAAGGG‑ATATTAAAGAAAAAAAACCCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGtt                                                                                                               <  2:201067/148‑1 (MQ=255)
                                        gAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTG‑TCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGG‑ATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTc                                                                                                              >  1:2713008/1‑150 (MQ=255)
                                        aaaaaTTTTTTGTAAAAAATATTGTTTCACCCGTGAGCGCATCATCTG‑TCAACAAAAGGGCGGGTGAGGGAGGAATGGGTCATAAAAAGGG‑ATTTTACAAAAAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTc                                                                                                              <  2:2614971/149‑1 (MQ=255)
                                         aaaaTATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTG‑TCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGG‑ATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCg                                                                                                             >  2:3746450/1‑150 (MQ=255)
                                          aaaTATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTG‑TCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGG‑ATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGa                                                                                                            >  1:1360453/1‑150 (MQ=255)
                                          aaaTATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTG‑TCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGG‑ATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGa                                                                                                            >  2:4673882/1‑150 (MQ=255)
                                          aaaTATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTG‑TCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGG‑ATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGa                                                                                                            >  1:409322/1‑150 (MQ=255)
                                           aaTATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTG‑TCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGG‑ATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGaa                                                                                                           >  1:1218159/1‑150 (MQ=255)
                                            atatTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTG‑TCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGG‑ATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAAc                                                                                                          >  1:4619335/1‑150 (MQ=255)
                                            atatTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTG‑TCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGG‑ATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAAc                                                                                                          >  1:1974817/1‑150 (MQ=255)
                                              tttttGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTG‑TCAACAAAATGGCAGGTGAAGGAGGCATAGGTCATAAAAATGG‑ATATTACAGAAAAAAAACCCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACAt                                                                                                        <  2:850841/149‑1 (MQ=255)
                                                ttttttCAAAATAATGTTCCATCCGGGAGCGCATCATCTG‑ACAACTAAAGGGGGGGGGAAGGAGGCATAGGTCATAAAAATGG‑ATTTTAAAAAAAAAAAACCCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATgg                                                                                                      <  2:1867852/150‑1 (MQ=255)
                                                  tGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTG‑TCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGG‑ATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCa                                                                                                    >  2:2522949/1‑150 (MQ=255)
                                                   gTTCACAATAATGTTTCATCCGTGAGCGCATCATCTG‑TCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGG‑ATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCag                                                                                                   >  1:4413883/1‑150 (MQ=255)
                                                   attCACAAGAATGTTTGATCCGTGATCGTA‑CAACGGCAAAACTAATTGGAAGGTGAGGGAGCCGGCGGTCTTACAAATGA‑ATATTAAAAAAAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCag                                                                                                   <  2:2454790/149‑1 (MQ=255)
                                                     tCACAATAATGTTTCATCCGTGAGCGCATCATCTG‑TCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGG‑ATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCagag                                                                                                 >  1:4119395/1‑150 (MQ=255)
                                                         aataatGTTTCATCCGTGAGCGCATCATCTG‑TCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGG‑ATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCg                                                                                             >  2:2948586/1‑150 (MQ=255)
                                                         aataatGTTTCATCCGTGAGCGCATCATCTG‑TCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGG‑ATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCg                                                                                             >  1:3335678/1‑150 (MQ=255)
                                                          ataatGTTTCATCCGTGAGCGCATCATCTG‑TCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGG‑ATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGa                                                                                            >  2:4554566/1‑150 (MQ=255)
                                                               gTTTAATCGGGGGGCGCATCATCTG‑TCAACAAAAGGGAAGGGGAAGGGGGAAAAGGTAATAAAAAGGG‑ATTTTACAGAAAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGAttctt                                                                                       <  2:944372/150‑1 (MQ=255)
                                                                 ttCATCCGTGAGCGCATCATCTG‑TCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGG‑ATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCg                                                                                     >  2:3892577/1‑150 (MQ=255)
                                                                  tCATCGGTGAGCACATCATCTG‑CAAAAAAAATGGCAGGGGAAGGAGGCATAGGTCATACAAAGGG‑ATATAAAAAAAAAAAAACCCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGt                                                                                    <  2:137778/150‑1 (MQ=255)
                                                                    acccGTGAGCGCACCATCTG‑TCAACAAAATGGCAGGGGAGGGAGGCTTAGTTAATACAAATGG‑ATATTACAAACAAAAAACCCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTg                                                                                  <  2:1834922/148‑1 (MQ=255)
                                                                     tCCGTGAGCGCATCATCTG‑TCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGG‑ATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGc                                                                                 >  2:2838477/1‑150 (MQ=255)
                                                                          gAGCGCATCATCTG‑TCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGG‑ATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATa                                                                            >  2:1782713/1‑150 (MQ=255)
                                                                           aGCGCATCATTTG‑TCAACAAAAGGGCAGGTGAGGGGGGCATAGGTCATAAAAATGG‑ATATTACAAAAAAAAAACCCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATac                                                                           <  2:309584/150‑1 (MQ=255)
                                                                           aGCGCATCATCTG‑TCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATAAAAAGGA‑ATATTACAAAAAAAAAAACCCGCCGCAGCGGGTCTTTAAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATac                                                                           <  2:3038047/150‑1 (MQ=255)
                                                                            gcacaaaatcTG‑ACAACAAAATGGCGGGTGAGGGAGGCAGAGGTCAAAAAAAGGT‑ATATTACACAAAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATacg                                                                          <  2:1925928/143‑1 (MQ=255)
                                                                             cgcATCATCTG‑TCAACTAAATGCGGGGTGAAGGAGGCATAGGTAATAAAAATGA‑ATATTACAAAAAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATacga                                                                         <  2:682577/150‑1 (MQ=255)
                                                                               catcatCTG‑TCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGG‑ATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATacgacg                                                                       >  2:56053/1‑150 (MQ=255)
                                                                                  catcTG‑TCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGG‑ATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAAt                                                                    >  1:569738/1‑150 (MQ=255)
                                                                                  catcTG‑TCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGG‑ATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGGTCGATTAGTGTTCGAACATGGCAGAAATCGACTCTTCGTTGCTTGTACGACTAAt                                                                    >  1:978513/1‑150 (MQ=255)
                                                                                   atcAG‑TCATCAAAATGCAGGGTGAAGGGGGCATAGAATAAAAACATGG‑ACATTACAAAAAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATc                                                                   <  2:778458/150‑1 (MQ=255)
                                                                                    tcTG‑TCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGG‑ATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCAGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCg                                                                  >  2:2049705/1‑150 (MQ=255)
                                                                                       g‑TCAACAAAAGGGCAGGTGAAGGAGGCATAGGTCATAAAAATGG‑ATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCtt                                                               <  1:2104574/150‑1 (MQ=255)
                                                                                         taaaCAAAAGGGCAGGGGAAGGAGGCATAGGTAATA‑AAATGGTTTATTAAAAAAAAAAAACCCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTc                                                              <  2:2272128/148‑1 (MQ=255)
                                                                                             cTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGG‑ATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGcc                                                          >  2:811325/1‑150 (MQ=255)
                                                                                               aaaGGGCAGGTGAAGGAGGAATAGGTCATAAAAATGG‑ATATAACAAAAAAAAAACCCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAg                                                        <  2:4425174/150‑1 (MQ=255)
                                                                                                aagggCAGGTGAGGGAGGCATAGGTCATAAAAATGG‑ATATTACAGAAAAAAAACCCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGc                                                       <  1:3439000/147‑1 (MQ=255)
                                                                                                  gggCAGGTGAAGGAGGCTGAGGAAAAACAAATGG‑ATATTACAGAAAAAAAAGCCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCAt                                                     <  1:1836899/149‑1 (MQ=255)
                                                                                                   ggCAGGTGAAGGAGGCATAGGTCATACAAATGG‑ATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATa                                                    >  2:3648682/1‑150 (MQ=255)
                                                                                                     ggggTGAAGGAGGCATGGGTCATACAAATGG‑ATTTTACAAAAAAAAAACCCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATAcc                                                  <  2:619253/148‑1 (MQ=255)
                                                                                                       ggTGAAGGAGGCATAGTTCATACAAATGG‑ATATTACAGACAAAAAACCCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTg                                                <  1:2495610/150‑1 (MQ=255)
                                                                                                       ggTGAAGGAGGCATAGGTCATACAAATGG‑ATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTg                                                >  1:3056851/1‑150 (MQ=255)
                                                                                                       ggTGAAGGAGGCATAGGTCATAAAAATGA‑ATATTAAAGAAAAAAAACCCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTg                                                <  1:3841832/150‑1 (MQ=255)
                                                                                                        ggaagggaggaATAGGTCATACAAATGA‑ATATAACAAAAAAAAAACCCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGa                                               <  1:4749484/145‑1 (MQ=255)
                                                                                                          gAAGGAGGCATAGGTCATACAAATGG‑ATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACa                                             >  2:1171459/1‑150 (MQ=255)
                                                                                                            aggaggCATGGGTCAAACAAATGA‑AAATTAAAAAAAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAgg                                           <  1:1762254/150‑1 (MQ=255)
                                                                                                             ggaggCATGGGTTATAAAAATGG‑ATATAACAAAAAAAAAACCCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAggg                                          <  1:2024900/150‑1 (MQ=255)
                                                                                                             ggaggCATAGGTCATACAAATGA‑ATATTACAGACAAAAAACCCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAggg                                          <  1:2559778/150‑1 (MQ=255)
                                                                                                              gaggCATAGGTCATACAAATGG‑ATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGt                                         >  1:1003975/1‑150 (MQ=255)
                                                                                                                  cATAGGTCATACAAATGG‑ATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCaga                                     >  1:2949005/1‑150 (MQ=255)
                                                                                                                     aGGTCATAAAAAGGA‑ATATAAAAGAAAAAAAACCCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTa                                  <  2:4236014/150‑1 (MQ=255)
                                                                                                                      ggTCATAAAAATGG‑ATATTACAAACAAAAAACCCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTAc                                 <  2:3220561/150‑1 (MQ=255)
                                                                                                                       gtaaTAAAAAGGG‑ATATTACAAAAAAAAAACCCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACg                                <  2:3831906/147‑1 (MQ=255)
                                                                                                                         cATACAAAGGT‑ATTTTACAGACAAAAAACCCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCa                              <  1:660771/150‑1 (MQ=255)
                                                                                                                          aTACAAATGT‑ATATCACAGACAAAAAACCCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCAc                             <  2:4298351/150‑1 (MQ=255)
                                                                                                                          aTACAAATGG‑ATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCAc                             >  1:2847757/1‑150 (MQ=255)
                                                                                                                          aTACAAATGG‑ATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCAc                             >  1:3199840/1‑150 (MQ=255)
                                                                                                                           tACAAATGG‑ATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCACg                            >  2:2731464/1‑150 (MQ=255)
                                                                                                                            aaaaaTGG‑ATATTAAAAAAAAAAAACCCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCACGt                           <  2:4011796/148‑1 (MQ=255)
                                                                                                                            aCAAATGG‑ATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCACGt                           >  1:3752352/1‑150 (MQ=255)
                                                                                                                              aaaTGG‑ATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGATCGATTAGTGATCGAACATGGCAGAGATCGATTCTTCGTTACTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTAACCACGTTc                         >  2:405828/1‑150 (MQ=255)
                                                                                                                              aaaTGA‑ATATCACAAAAAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCACGTTc                         <  2:329475/150‑1 (MQ=255)
                                                                                                                               aaTGG‑ATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCACGTTCg                        >  2:1490908/1‑150 (MQ=255)
                                                                                                                                     atatTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCACGTTCGGCAGt                   >  1:824444/1‑150 (MQ=255)
                                                                                                                                      tatTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCACGTTCGGCAGtg                  >  2:2713327/1‑150 (MQ=255)
                                                                                                                                       atTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCACGTTCGGCAGtga                 >  1:4268589/1‑150 (MQ=255)
                                                                                                                                           cAGAAAAAAAACCCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCACGTTCGGCAGtgatttg             <  1:419993/150‑1 (MQ=255)
                                                                                                                                             gaaaaaaaacCCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCACGTTCGGCAGtgatttgat           <  1:2276794/147‑1 (MQ=255)
                                                                                                                                              aaaaaaaacCCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCACGTTCGGCAGtgatttgatt          <  2:4600881/148‑1 (MQ=255)
                                                                                                                                                 aaaaacCCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCACGTTCGGCAGTGATTTGATTTCa       <  1:2627861/150‑1 (MQ=255)
                                                                                                                                                 aaaaaaCCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCACGTTCGGCAGTGATTTGATTTCa       >  2:751232/1‑150 (MQ=255)
                                                                                                                                                  aaaaaCCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCACGTTCGGCAGTGATTTGATTTCAt      >  1:1052321/1‑150 (MQ=255)
                                                                                                                                                   aaaaCCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCACGTTCGGCAGTGATTTGATTTCATc     >  1:1496594/1‑150 (MQ=255)
                                                                                                                                                     aaCCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCACGTTCGGCAGTGATTTGATTTCATCGc   >  2:2485529/1‑150 (MQ=255)
                                                                                                                                                     aaCCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCACGTTCGGCAGTGATTTGATTTCATCGc   >  1:4589870/1‑150 (MQ=255)
                                                                                                                                                      aCCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCACGTTCGGCAGTGATTTGATTTCATCGCt  >  1:3287570/1‑150 (MQ=255)
                                                                                                                                                      |                                                                                                                                                     
GCAAGCGTCGATCAGAGCGCGGAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTG‑TCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGG‑ATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCACGTTCGGCAGTGATTTGATTTCATCGCT  >  AP012306/1066175‑1066472

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑