Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123061,309,9140AC32.7% 289.4 / 11.1 150L23F (TTA→TTC) lsrCAI2 transporter
Reads supporting (aligned to +/- strand):  ref base A (58/43);  new base C (0/49);  total (58/92)
Fisher's exact test for biased strand distribution p-value = 3.59e-14
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 224 total aligned reads displayed.

CTCTGCACTGACCGAGCGCGATATTAATGTCGAGACTATTATGCGCGTTGCCTTCGGCGATAGTCAGCGTCAGGAGGCGTCATGCTGAAGTTTATTCAGAACAACCGTGAAATCACGGCACTGCTGGCGGTGGTGCTGCTGTTTGTATTACCCGGTTTTCTCGACCGCCAGTATTTAAGTGTGCAAACGCTGACCATGGTTTATAGCAGCGCGCAAATCCTGATCCTGCTGGCAATGGGCGCGACGCTGGTAATGCTTACGCGCAATATTGATGTTTCAGTGGGTTCGATTACCGGAAT  >  AP012306/1309765‑1310063
                                                                                                                                                     |                                                                                                                                                     
ctctGCACTGACCGAGCGCGATATTAATGTCGAGACTATTATGCGCGTTGCCTTCGGCGATAGTCAGCGTCAGGAGGCGTCATGCTGAAGTTTATTCAGAACAACCGTGAAATCACGGCACTGCTGGCGGTGGTGCTGCTGTTTGTATTa                                                                                                                                                       >  2:230632/1‑150 (MQ=255)
ctctGCACTGACCGAGCGCGATATTAATGTCGAGACTATTATGCGCGTTGCCTTCGGCGATAGTCAGCGTCAGGAGGCGTCATGCTGAAGTTTATTCAGAACAACCGTGAAATCACGGCACTGCTGGCGGTGGGGCTGCTGTTTGTATTa                                                                                                                                                       >  2:171025/1‑150 (MQ=255)
  ctGCACTGACCGAGCGCGATATTAATGTCGAGACTATTATGCGCGTTGCCTTCGGCGATAGTCAGCGTCAGGAGGCGTCATGCTGAAGTTTATTCAGAACAACCGTGAAATCACGGCACTGCTGGCGGTGGTGCTGCTGTTTGTATTAcc                                                                                                                                                     <  1:802213/150‑1 (MQ=255)
    gCACTGACCGAGCGCGATATTAATGTCGAGACTATTATGCGCGTTGCCTTCGGCGATAGTCAGCGTCAGGAGGCGTCATGCTGAAGTTTATTCAGAACAACCGAGAAATCACGGCACTGCTGGCGGTGGTGCTGCTGTTTGTATTACCCg                                                                                                                                                   <  2:4200090/150‑1 (MQ=255)
          aCCGAGCGCGATATTAATGTCGAGACTATTATGCGCGTTGCCTTCGGCGATAGTCAGCGTCAGGAGGCGTCATGCTGAAGTTTATTCAGAACAACCGTGAAATCACGGCACTGCTGGCGGTGGTGCTGCTGTTTGTATTACCCGGTTTtc                                                                                                                                             >  1:2550820/1‑150 (MQ=255)
           ccGAGCGCGATATTAATGTCGAGACTATTATGCGCGTTGCCTTCGGCGATAGTCAGCGTCAGGAGGCGTCATGCTGAAGTTTATTCAGAACAACCGTGAAATCACGGCACTGCTGGCGGTGGTGCTGCTGTTTGTATTACCCGGTTTtct                                                                                                                                            <  2:2867806/150‑1 (MQ=255)
            cGAGCGCGATATTAATGTCGAGACTATTATGCGCGTTGCCTTCGGCGATAGTCAGCGTCAGGAGGCGTCATGCTGAAGTTTATTCAGAACAACCGTGAAATCACGGCACTGCTGGCGGTGGTGCTGCTGTTTGTATTACCCGGTTTtctc                                                                                                                                           <  2:754195/150‑1 (MQ=255)
            cGAGCGCGATATTAATGTCGAGACTATTATGCGCGTTGCCTTCGGCGATAGTCAGCGTCAGGAGGCGTCATGCTGAAGTTTATTCAGAACAACCGTGAAATCACGGCACTGCTGGCGGGGGTGGTGCTTTTTGTATTTCCCGGTTTtttc                                                                                                                                           >  2:4586344/1‑150 (MQ=255)
             gAGCGCGATATTAATGTCGAGACTATTATGCGCGTTGCCTTCGGCGATAGTCAGCGTCAGGAGGCGTCATGCTGAAGTTTATTCAGAACAACCGTGAAATCACGGCACTGCTGGCGGTGGTGCTGCTGTTTGTATTACCCGGTTTTCTCg                                                                                                                                          <  1:2164236/150‑1 (MQ=255)
             gAGCGCGATATTAATGTCGAGACTATTATGCGCGTTGCCTTCGGCGATAGTCAGCGTCAGGAGGCGTCATGCTGAAGTTTATTCAGAACAACCGTGAAATCACGGCACTGCTGGCGGTGGGGCTGCTGTTTGTATTACCCGGTTTTCTCg                                                                                                                                          >  2:1905165/1‑150 (MQ=255)
                  cgATATTAATGTCGAGACTATTATGCGCGTTGCCTTCGGCGATAGTCAGCGTCAGGAGGCGTCATGCTGAAGTTTATTCAGAACAACCGTGAAATCACGGCACTGCTGGCGGTGGTGCTGCTGTTTGTATTACCCGGTTTTCTCGACCGc                                                                                                                                     >  2:3236984/1‑150 (MQ=255)
                  cgATATTAATGTCGAGACTATTATGCGCGTTGCCTTCGGCGATAGTCAGCGTCAGGAGGCGTCATGCTGAAGTTTATTCAGAACAACCGTGAAATCACGGCACTGCTGGCGGTGGGGCTGCTGTTTGTATTACCCGGTTTTCCCGACCGc                                                                                                                                     >  2:1645166/1‑150 (MQ=255)
                  cgATATTAATGTCGAGACTATTATGCGCGTTGCCTTCGGCGATAGTCAGCGTCAGGAGGCGTCATGCTGAAGTTTATTCAGAACAACCGTGAAATCACGGCACTGCTGGCGGAGGGGGCGCTGTTTGTATTACCCGGGTTTTTCGAcccc                                                                                                                                     >  1:1125732/1‑148 (MQ=255)
                   gATATTAATGTCGAGACTATTATGCGCGTTGCCTTCGGCGATAGTCAGCGTCAGGAGGCGTCATGCTGAAGTTTATTCAGAACAACCGTGAAATCACGGCACTGCTGGCGGTGGTGCTGCTGTTTGTATTACCCGGTTTTCTCGACCGcc                                                                                                                                    >  2:2759559/1‑150 (MQ=255)
                    atatTAATGTCGAGACTATTATGCGCGTTGCCTTCGGCGATAGTCAGCGTCAGGAGGCGTCATGCTGAAGTTTATTCAGAACAACCGTGAAATCACGGCACTGCTGGCGGTGGTGCTGCTGTTTGTATTACCCGGTTTTCTCGACCGCCa                                                                                                                                   <  1:13648/150‑1 (MQ=255)
                     tatTAATGTCGAGACTATTATGCGCGTTGCCTTCGGCGATAGTCAGCGTCAGGAGGCGTCATGCTGAAGTTTATTCAGAACAACCGTGAAATCACGGCACTGCTGGCGGTGGTGCTGCTGTTTGTATTACCCGGTTTTCTCGACCGCCAg                                                                                                                                  >  1:1807607/1‑150 (MQ=255)
                       ttAATGTCGAGATTTTTATTCGCGTTGCCTTCGGCGATAGTCAGCGTCAGGGGGCGTCATGCTGAATTTTATTCAGAACAACCGTGAAATCCCGGCACTGCTGGGGGTGGTGCTGCTGTTTGTATTCCCCGGTTTTCTCGACCGCCAGTa                                                                                                                                <  2:2773644/150‑1 (MQ=255)
                           tGTCGAGACTATTATGCGCGTTGCCTTCGGCGATAGTCAGCGTCAGGAGGCGTCATGCTGAAGTTTATTCAGAACAACCGTGAAATCACGGCACTGCTGGCGGTGGTGCTGCTGTTTGTATTACCCGGTTTTCTCGACCGCCAGTATTTa                                                                                                                            >  1:4207309/1‑150 (MQ=255)
                             tCGAGACTATTATGCGCGTTGCCTTCGGCGATAGTCAGCGTCAGGAGGCGTCATGCTGAAGTTTATTCAGAACAACCGTGAAATCACGGCACTGCTGGCGGGGGTGCTGCTGGTTGTATTACCCGGTTTTTTCGACCGCCAGTATTTAAg                                                                                                                          >  2:2890532/1‑150 (MQ=255)
                              cGAGACTATTATGCGCGTTGCCTTCGGCGATAGTCAGCGTCAGGAGGCGTCATGCTGAAGTTTATTCAGAACAACCGTGAAATCACGGCACTGCTGGCGGTGGTGCTGCTGTTTGTATTACCCGGTTTTCTCGACCGCCAGTATTTAAgt                                                                                                                         <  1:1926668/150‑1 (MQ=255)
                                agaCTATTATGCGCGTTGCCTTCGGCGATAGTCAGCGTCAGGAGGCGTCATGCTGAAGTTTATTCAGAACAACCGTGAAATCACGGCACTGCTGGCGGTGGTGCTGCTGTTTGTATTACCCGGGTTTCTCGACCGCCAGTATTTAAgtgt                                                                                                                       >  1:59771/1‑150 (MQ=255)
                                  aCTATTATGCGCGTTGCCTTCGGCGATAGTCAGCGTCAGGAGGCGTCATGCTGAAGTTTATTCAGAACAACCGTGAAATCACGGCACTGCTGGCGGTGGTGCTGCTGTTTGTATTCCCCGGTTTTCTCGACCGCCAGTATTTAAGTGTGc                                                                                                                     <  1:407668/150‑1 (MQ=255)
                                    tattatGCGCGTTGCCTTCGGGGATAGTCAGCGGCAGGAGGCGTCATGCTGAAGTTTATTCAGAACAACCGTGAAATCACGGCACTGCTGGGGGTGGTGCTGCTGTTTGTATTCCCCGTTTTTCTCGACCGCCAGTATTTAAGTGTGCaa                                                                                                                   <  2:2665976/150‑1 (MQ=255)
                                    tattatGCGCGTTGCCTTCGGCGATAGTCAGCGTCAGGAGGCGTCATGCTGAAGTTTATTCAGAACAACCGTGAAATCACGGCACTGCTGGCGGTGGTGCTGCTGTTTGTATTACCCGGTTTTCTCGACCGCCAGTATTTAAGTGTGCaa                                                                                                                   >  2:226277/1‑150 (MQ=255)
                                     tttatGCGCTTTGCCTTGGGCGATAGTCAGCGTCAGGAGGCGTCATGCTGAATTTTATTCAGAACAACCGTGAAATCACGGCACTGCTGGCGGTGGTGCTGCTGTTTGTATTACCCGGTTTTCTCGACCGCCAGTATTTAAGTGTGCaaa                                                                                                                  <  2:2222646/149‑1 (MQ=255)
                                     attatGCGCGTTGCCTTCGGCGATAGTCAGCGTCAGGAGGCGTCATGCTGAAGTTTATTCAGAACAACCGTGAAATCACGGCACTGCTGGCGGTGGTGCTGCTGTTTGTATTACCCGGTTTTCTCGACCGCCAGTATTTAAGTGTGCaaa                                                                                                                  <  1:29621/150‑1 (MQ=255)
                                     attatGCGCGTTGCCTTCGGCGATAGTCAGCGTCAGGAGGCGTCATGCTGAAGTTTATTCAGAACAACCGTGAAATCACGGCACTGCTGGCGGTGGTGCTGCTGTTTGTATTACCCGGGTTTCTCGACCGCCCGTATTTAAGTGTGCaaa                                                                                                                  >  2:2068682/1‑150 (MQ=255)
                                       ttggcgcGTTGCCTTCGGCGATAGTCAGCGTCAGGAGGCGTCATGCTGAAGTTTATTCAGAACACCCGTGAAATCACGGCACTGCTGGGGGTGGTGCTGCTGTTTGTATTCCCCGGTTTTCTCGACCGCCAGTATTTAAGTGTGCAAACg                                                                                                                <  2:3680195/147‑1 (MQ=255)
                                             cgTTGCTTTCGGCGATAGTCAGCGTCAGGAGGCGTCATGCTGAAGTTTATTCACAACACCCGTGAAATCACGGCACTGCTGGCGGTGGTGCTGCTGTTTGTATTACCCGGTTTTCTCGACCGCCAGTATTTAAGTGTGCAAACGCTGAcc                                                                                                          <  1:178206/150‑1 (MQ=255)
                                              gTTGCCTTCGGCGATAGTCAGCGTCAGGAGGCGTCATGCTGAAGTTTATTCAGAACAACCGTGAAATCACGGCACTGCTGGCGGTGGTGCTGCTGTTTGTATTCCCCGGTTTTCTCGACCGCCAGTATTTAAGTGTGCAAACGCTGACCa                                                                                                         <  1:3674339/150‑1 (MQ=255)
                                              gTTGCCTTCGGCGATAGTCAGCGTCAGGAGGCGTCATGCTGAAGTTTATTCAGAACAACCGTGAAATCACGGCACTGCTGGCGGTGGTGCTGCTGTTTGTATTACCCGGTTTTCTCGACCGCCAGTATTTAAGTGTGCAAACGCTGACCa                                                                                                         >  2:964076/1‑150 (MQ=255)
                                                 gCCTTCGGCGATAGTCAGCGTCAGGAGGCGTCATGCTGAAGTTTATTCAGAACACCCGTGAAATCACGGCACTGCTGGCGGTGGTGCTGCTGTTTGTATTCCCCGGTTTTCTCGACCGCCAGTATTTAAGTGTGCAAACGCTGACCATgg                                                                                                      <  2:4504300/150‑1 (MQ=255)
                                                  ctttgggCAATAGCCAGCGTCGGGGGGCGTCTTGCTAAATTTTTTCAAAAACACCCGTAAAACCCCGGCCCTGCGGGCGGGGGTGCTGTTTTTTTTATTCCCCGTTTTTCTCGACCGCCAGTATTTAAGTGTGCAAACGCTGACCATGGt                                                                                                     <  2:4748127/145‑1 (MQ=255)
                                                     tgggCGATAGTCAGCGTCAGGAGGCGTCATGCTGAAGTTTATTCAGAACAACCGTGAAATCACGGCACTGCTGGCGGTGGTGCTGCTGTTTGTATTCCCCGTTTTTCTCGACCGCCAGTATTTAAGTGTGCAAACGCTGACCATGGTTta                                                                                                  <  2:3345337/148‑1 (MQ=255)
                                                                 aGCGTCAGGAGGCGTCATGCTGAAGTTTATTCAGAACAACCGTGAAATCACGGCACTGCTGGCGGTGGTGCTGCTGTTTGTATTACCCGGTTTTCTCGACCGCCAGGATTTAAGTGTGCAAACGCTGACCCTGGTTTATAGCAGCGCGCa                                                                                      >  2:216468/1‑150 (MQ=255)
                                                                    gTCAGGAGGCGTCATGCTGAAGTTTATTCAGAACACCCGTGAAATCACGGCACTGCGGGCGGTGGTGCTGCTGTTTGTATTCCCCGGTTTTCTCGACCGCCAGTATTTAAGTGTGCAAACGCTGACCATGGTTTATAGCAGCGCGCAAat                                                                                   <  2:685817/150‑1 (MQ=255)
                                                                        ggcggCGTCATGCTGATGTTTATTAAAAACAAGCGTGAAATCACGGCTGTGCTGGCGGTGGTGCTGCTGTTTGTTTTACCCTGTTTTCTCGCCCGCCAGTATTTAAGTGTGCAAACGCTGACCATGGTTTATAGCAGCGCGCAAatcctg                                                                               <  2:1306420/147‑1 (MQ=255)
                                                                         gaggCGTCATGCTGAAGTTTATTCAGAACAACCGTGAAATCACGGCACTGCTGGCGGTGGTGCTGCTGTTTGTATTACCCGGTTTTCTCGACCGCCAGTATTTAAGTGTGCAAACGCTGACCATGGTTTATAGCAGCGCGCAAatcctga                                                                              <  1:3444438/150‑1 (MQ=255)
                                                                           ggCGTCATGCTGAAGTTTATTCAGAACAACCGTGAAATCACGGCACTGCTGGGGGTGGTGCTGCTGTTTGTATTCCCCGGTTTTCTCGACCGCCAGTATTTAAGTGTGCAAACGCTGACCATGGTTTATAGCAGCGCGCAAatcctgatc                                                                            <  2:3266157/150‑1 (MQ=255)
                                                                           ggCGTCATGCTGAAGTTTATTCAGAACAACCGTGAAATCACGGCACTGCTGGCGGGGGGGCTGCTGTTTGTATTACCCGGTTTTCTCGACCGCCAGTATTTAAGTGTGCAAACGCTGACCATGGTTTTTAGCAGCGCGCAAatcctgatc                                                                            >  2:1247876/1‑150 (MQ=255)
                                                                            gCGTCATGCTGAAGTTTATTCAGAACAACCGTAAAATCACGGCACTGCTGGCGGTGGTGCCGCTGTTTGTATTCCCCGGTTTTCTCGACCGCCAGTATTTAAGTGTGCAAACGCTGACCATGGTTTATAGCAGCGCGCAAatcctgatcc                                                                           <  1:3334553/150‑1 (MQ=255)
                                                                             cGTCATGCTGAAGTTTATTCAGAACAACCGTGAAATCACGGCACTGCTGGCGGTGGTGCTGCTGTTTGTATTACCCGGTTTTCTCGACCGCCAGTATTTAAGTGTGCAAACGCTGACCATGGTTTATAGCAGCGCGCAAatcctgatcct                                                                          >  1:1560058/1‑150 (MQ=255)
                                                                              gTCATGCTGAAGTTTATTCAGAACAACCGTGAAATCACGGCACTGCTGGCGGTGGTGCTGCTGTTTGTATTCCCCGGTTTTCTCGACCGCCAGTATTTAAGTGTGCAAACGCTGACCATGGTTTATAGCAGCGCGCAAatcctgatcctg                                                                         <  2:2704610/150‑1 (MQ=255)
                                                                                   gCTGAGGTTTATCCAAAACAACCGTGAAATCACGGCCCTGCGGGCGGGGGTGCTGTTTTTTGTATTCCCCGGTTTTCTCGACCGCCAGTATTTAAGTGTGCAAACGCTGACCATGGTTTATAGCAGCGCGCAAATCCTGATCCTGCTGGc                                                                    <  2:4443850/150‑1 (MQ=255)
                                                                                      gAAGTTTATTCAGAACAACCGTGAAATCACGGCACTGCTGGCGGTGGTGCTGCTGTTTGTATTACCGGGTTTTCTCGACCGCCAGTATTTAAGTGTGCAAACGCTGACCATGGTTTATAGCAGCGCGCAAATCCTGATCCTGCTGGCAAt                                                                 <  1:889408/150‑1 (MQ=255)
                                                                                      gAAGTTTATTCAGAACAACCGTGAAATCACGGCACTGCTGGCGGTGGTGCTGCTGTTTGTATTACCCGGTTTTCTCGACCGCCAGTATTTAAGTGTGCAAACGCTGACCATGGTTTATAGCAGCGCGCAAATCCTGATCCTGCTGGCAAt                                                                 <  1:2660041/150‑1 (MQ=255)
                                                                                         ttttATTCAGAACACCCGTGAAATCGGGGCCCTGCTGGGGGGGGTGCTGGTGTTTGTTTTCCCTGTTTTTCGCGACCGCCAGTATTTAAGTGTGCAAACGCTGACCATGGTTTATAGCAGCGCGCAAATCCTGATCCTGCTGGCAATggg                                                              <  2:4686173/149‑1 (MQ=255)
                                                                                         gTTTATTCAGAACAACCGTGAAATCAGGGCACTGCGGGGGGGGGTGCTGCTGTTTGTATTACCCGGTTTTCTCGACCGCCAGTATTTAAGTGTGCAAACGCTGACCATGGTTTATAGCAGCGCGCAAATCCTGATCCTGCTGGCAATggg                                                              <  2:1319482/150‑1 (MQ=255)
                                                                                           ttATTCAGAACAACCGTGAAATCACGGCACTGCTGGCGGAGGTTGTGTTGTTTTTATTACCCGGTTTTCTCGATCGCCCGCATTTAAGAGGGCAAACGCTGAGCAATGGTTATAGGAGCGTCCAAATCCTGCTCCTGGTGGAAATGGgcg                                                            >  2:3557567/1‑150 (MQ=255)
                                                                                            tATTCAGAACACCCGTGAAATCACGGCACTGCTGGCGGTGGTGCTGTTGTTTGTATTACCCGGTTTTCTCGACCGCCAGTATTTAAGTGTGCAAACGCTGACCATGGTTTATAGCAGCGCGCAAATCCTGATCCTGCTGGCAATGGgcgc                                                           <  1:320366/150‑1 (MQ=255)
                                                                                             aTTCAGAACAACCGTGAAATCACGGCACTGCTGGCGGTGGTGCTGCTGTTTGTATTACCCGGTTTTCTCGACCGCCAGTATTTAAGTGTGCAAACGCTGACCATGGTTTATAGCAGCGCGCAAATCCTGATCCTGCTGGCAATGGGcgcg                                                          >  1:4243647/1‑150 (MQ=255)
                                                                                               tCAGAACAACCGTGAAATCACGGCACTGCTGGCGGTGGGGCTGCTGTTTGTATTACCCGGGTTTCTCGACCGCCAGTATTTAAGTGTGCAAACGCTGACCATGGTTTATAGCAGCGCGCAAATCCTGATCCTGCTGGCAATGGGCGCGAc                                                        >  1:3604835/1‑150 (MQ=255)
                                                                                               tCAGAACAACCGTGAAATCACGGCACTGCTGGCGGCGGGGCTGCTGTTTTTCTTACCCGGGTTTCTCGACCGCCCGTATTTAAGGCTGCAAAAGCTGGCCTTGGTTTATAGCAGGGCGCAAATCCTGATCCTGCTGGCAATGGGcgcggc                                                        >  2:1958131/1‑148 (MQ=255)
                                                                                                   aacaacCGTGAAATCACGGCACGGCTGGCGGTGGTGCTGCTGTTTGTATTACCCGGTTTTCTCGACCGCCAGTATTTAAGTGTGCAAACGCTGACCATGGTTTATAGCAGCGCGCAAATCCTGATCCTGCTGGCAATGGGCGCGACGCTg                                                    <  1:1478708/150‑1 (MQ=255)
                                                                                                      aacCGTGAAATCACGGCACTGCTGGCGGTGGTGCTGCTGTTTGTATTACCCGGGTTTCTCGACCGCCAGTATTTAAGTGTGCAAACGCTGACCATGGTTTATAGCAGCGCGCAAATCCTGATCCTGCTGGCAATGGGCGCGACGCTGGTa                                                 >  2:435732/1‑150 (MQ=255)
                                                                                                      aacCGTAAAATCCGGGCACTGCGGGCGGTGGTGCTGCTTTTTGTATTCCCCGTTTTTCTCGACCGCCAGTATTTAAGTGTGCAAACGCTGACCATGGTTTATAGCAGCGCGCAAATCCTGATCCTGCTGGCAATGGGCGCGACGCTGGTa                                                 <  2:4304066/150‑1 (MQ=255)
                                                                                                        ccGTGAAATCACGGCACTGCTGGCGGTGGTGCTGCTGTTTGTATTCCCCGGTTTTCTCGACCGCCAGTATTTAAGTGTGCAAACGCTGACCATGGTTTATAGCAGCGCGCAAATCCTGATCCTGCTGGCAATGGGCGCGACGCTGGTAAt                                               <  2:2819657/150‑1 (MQ=255)
                                                                                                        ccGTGAAATCACGGCACTGCTGGCGGTGGTGCTGCTGTTTGTATTCCCCGGTTTTCTCGACCGCCAGTATTTAAGTGTGCAAACGCTGACCATGGTTTATAGCAGCGCGCAAATCCTGATCCTGCTGGCAATGGGCGCGACGCTGGTAAt                                               <  1:3264036/150‑1 (MQ=255)
                                                                                                         cGTGAAATCAGGGCACTGGTGGGGGTGGTGCTGCTGTTTGTATTACCCGTTTTTCTCGACCGCCAGTATTTAAGTGTGCAAACGCTGACCATGGTTTATAGCAGCGCGCAAATCCTGATCCTGCTGGCAATGGGCGCGACGCTGGTAATg                                              <  2:2419558/150‑1 (MQ=255)
                                                                                                         cGTGAAATCACGGCACTGCTGGTGGTGTTGCTGTTGTTTGTATTACCCGGTTTTCTCGCCCGCCAGTATTTAAGTGTGCAAACGCTGACCATGGTTTATAGCAGCGCGCAAATCCTGATCCTGCTGGCAATGGGCGCGACGCTGGTAATg                                              <  1:3112546/150‑1 (MQ=255)
                                                                                                         cGTGAAATCACGGCACTGCTGGCGGTGGTGCTGCTGTTTGTATTACCCGGTTTTCTCGACCGCCAGTATTTAAGTGTGCAAACGCTGACCATGGTTTATAGCAGCGCGCAAATCCTGATCCTGCTGGCAATGGGCGCGACGCTGGTAATg                                              <  1:3007923/150‑1 (MQ=255)
                                                                                                          gtaaaaTCACGGCACTGCTGGCGGTGGTGCTGCTGTTTGTATTCCCCGGTTTTCTCGACCGCCAGTATTTAAGTGTGCAAACGCTGACCATGGTTTATAGCAGCGCGCAAATCCTGATCCTGCTGGCAATGGGCGCGACGCTGGTAATGc                                             <  1:3687187/147‑1 (MQ=255)
                                                                                                           tcaaaCCACGGCACTGCGGGGGGTGGTGTTGCTGTTTGTATTCCCCGGTTTTCTCGACCGCCAGTATTTAAGTGTGCAAACGCTGACCATGGTTTATAGCAGCGCGCAAATCCTGATCCTGCTGGCAATGGGCGCGACGCTGGTAATGCt                                            <  1:2833607/148‑1 (MQ=255)
                                                                                                           tGAAATCACGGCACTGCTGGCGGTGGGGCTGCTGTTTGTATTACCCGGTTTTCTCGACCGCCCGTATTTAAGTGGGCAAACGCTGACCATGGTTTTTAGCAGCGCGCAAATCCTGATCCTGCTGGCAATGGGCGCGACGCTGGTAATGCt                                            >  2:803940/1‑150 (MQ=255)
                                                                                                            gAAATCACGGCACTGCTGGCGGTGGTGCTGCTGTTTGTATTACCCGGTTTTCTCGACCGCCAGTATTTAAGTGTGCAAACGCTGACCATGGTTTATAGCAGCGCGCAAATCCTGATCCTGCTGGCAATGGGCGCGACGCTGGTAATGCtt                                           <  1:464316/150‑1 (MQ=255)
                                                                                                            gAAAGCACGGCAGTGGTGGCGGTGGTGCTGCTGTTTGTATTACCGGGTTTTCGCGACCGCCAGTATTTAAGTGTGCAAACGCTGACCATGGTTTATAGCAGCGCGCAAATCCTGATCCTGCTGGCAATGGGCGCGACGCTGGTAATGCtt                                           <  1:551377/150‑1 (MQ=255)
                                                                                                             aaaTCACGGCACTGCTGGCGGTGGTGCTGCTGTTTGTATTACCCGGTTTTCTCGACCGCCAGTATTTAAGTGTGCAAACGCTGACCATGGTTTATAGCAGCGCGCAAATCCTGA‑CCTGCTGGGAAATGGCGCGACGCTGGTAATGCTTAc                                         >  1:2616510/1‑150 (MQ=255)
                                                                                                             aaaTCACGGCACTGCTGGCGGTGGTGCTGCTGTTTGTATTACCCGGTTTTCTCGACCGCCAGTATTTAAGTGTGCAAACGCTGACCATGGGTTATAGCAGCGCGCCAATCCTGATCCTGCTGGCAATGGGCGCGACGCTGGTAATGCttt                                          >  2:1777189/1‑149 (MQ=255)
                                                                                                              aaTCACGGCCCGGCGGGCGGTGGTGCTGCTGTTTGTATTCCCCGGTTTTCTCGACCGCCAGTATTTAAGTGTGCAAACGCTGACCATGGTTTATAGCAGCGCGCAAATCCTGATCCTGCTGGCAATGGGCGCGACGCTGGTAATGCTTAc                                         <  1:2284972/150‑1 (MQ=255)
                                                                                                                tCACGGCACTGCTGGCGGTGGTGCTGCTGTTTGTATTATCCGGGTTTCTCGACCGCCAGTATTTAAGTGTGCAAACGCTGACCATGGGTTATAGCAGCGCGCAAATCCTGATCCTGCTGGCAATGGGCGCGACGCTGGTAATGCTTAcgc                                       >  1:2039255/1‑150 (MQ=255)
                                                                                                                    ggCACTGCTGGCGGTGGTGCTGCTGTTTGTATTCCCCGGTTTTCTCGACCGCCAGTATTTAAGTGTGCAAACGCTGACCATGGTTTATAGCAGCGCGCAAATCCTGATCCTGCTGGCAATGGGCGCGACGCTGGTAATGCTTACGCGCaa                                   <  2:2966512/150‑1 (MQ=255)
                                                                                                                    ggCACTGCTGGCGGTGGTGCTGCTGTTTGTATTACCCGGTTTTCTCGACCGCCAGTATTTAAGTGTGCAAACGCTGACCATGGTTTATAGCAGCGCGCAAATCCTGATCCTGCTGGCAATGGGCGCGACGCTGGTAATGCTTACGCGCaa                                   >  1:4630157/1‑150 (MQ=255)
                                                                                                                      cACTGCTGGCGGTGGTGCTGCTGTTTGTATTACCCGGTTTTCTCGACCGCCAGTATTTAAGTGTGCAAACGCTGACCATGGTTTATAGCAGCGCGCAAATCCTGATCCTGCTGGCAATGGGCGCGACGCTGGTAATGCTTACGCGCAata                                 <  1:3358907/150‑1 (MQ=255)
                                                                                                                      cACTGCTGGCGGTGGGGCCGCTGGTTTTATTACCCGGTTTTCTCGGCCGCCCGGATTTAAGTGGGCCAACGCTGACCATGGGTTATTGCAACGCGCAAATCCTGATCCTGCTGGCAATGGGCGCGACGCCGGTAATGCATTCGCGCCata                                 >  2:280469/1‑150 (MQ=255)
                                                                                                                        ctgctgGCGGTGGTGCTGCTGTTTGTATTTCCCGGTTTTCTCGACCGCCAGTATTTTAGTGTGCAAACGCTGACCATGGTTTATAGCAGCGCGCAAATCCTGGTCCTGCTGGCAATTGGCGCGCCGCTGGGAATGCTTACGCGCGATAtt                               >  2:4540688/1‑150 (MQ=255)
                                                                                                                        ctgctgGCGGTGGTGCTGCTGTTTGTATTACCCGGTTTTCTCGACCGCCAGTATTTAAGTGTGCAAACGCTGACCATGGTTTATAGCAGCGCGCAAATCCTGATCCTGCTAGCAATGGGCGCGACGCTGGTAATGCTTACGCGCAATAtt                               <  1:3748580/150‑1 (MQ=255)
                                                                                                                         tgctgGCGGTGGTGCTGCTTTTTGTATTACCCGTTTTTCTCGACCGCCAGTATTTAAGTGTGCAAACGCTGACCATGGTTTATAGCAGCGCGCAAATCCTGATCCTGCTGGCAATGGGCGCGACGCTGGTAATGCTTACGCGCAATATTg                              <  2:1177870/150‑1 (MQ=255)
                                                                                                                         tgctgGCGGTGGTGCTGCTGTTTGTATTCCCCGGTTTTCTCGACCGCCAGTATTTAAGTGTGCAAACGCTGACCATGGTTTATAGCAGCGCGCAAATCCTGATCCTGCTGGCAATGGGCGCGACGCTGGTAATGCTTACGCGCAATATTg                              <  1:2002901/150‑1 (MQ=255)
                                                                                                                              gCGGTGTTGTTGCTGTTTGTATTCCGCGGTTTACCAGACCGCCAGTATTTAAGTGTGCAAACGCTGACCATGGTTTATAGCAGCGCGCAAATCCTGATCCTGCTGGAAATGGGCGCGACGCTGGTAATGCTTACGCGCAATATTGATGtt                         <  2:1419234/150‑1 (MQ=255)
                                                                                                                                 gtggtgCTGTTTTTTGTATTCCCCGGTTTTCTCGACCGCCAGTATTTAAGTGTGCAAACGCTGACCATGGTTTATAGCAGCGCGCAAATCCTGATCCTGCTGGCAATGGGCGCGACGCTGGTAATGCTTACGCGCAATATTGATGTTTCa                      <  2:1587441/150‑1 (MQ=255)
                                                                                                                                      gctgctGTTTGTATTACCCGGTTTTCTCGACCGCCAGTATTTAAGTGTGCAAACGCTGACCATGGTTTATAGCAGCGCGCAAATCCTGATCCTGCTGGCAATGGGCGCGACGCTGGTAATGCTTACGCGCAATATTGATGTTTCAGTggg                 >  1:147235/1‑150 (MQ=255)
                                                                                                                                      gctgctGTTTGTATTACCCGGTTTTCTCGACCGCCAGTATTTAAGTGTGCAAACGCTGACCATGGTTTATAGCAGCGCGCAAATCCTGATCCTGCTGGCAATGGGCGCGACGCTGGTAATGCTTACGCGCAATATTGATGTTTCAGTggg                 >  2:4731687/1‑150 (MQ=255)
                                                                                                                                       ctgctgTTTGTATTACCCGGTTTTCTCGACCGCCAGTATTTAAGTGTGCAAACGCTGACCATGATTTATAGCAGCGCGCAAATCCTGATCCTGCTGGCAATGGGCGCGACGCTGGTAATGCTTACGCGCAATATTGATGTTTCAGTGGGt                >  2:4086845/1‑150 (MQ=255)
                                                                                                                                        tgttttttGTATTCCCCGTTTTTCTCGACCGCCAGTATTTAAGTGTGCAAACGCTGACCATGGTTTATAGCAGCGCGCAAATCCTGATCCTGCTGGCAATGGGCGCGACGCTGGTAATGCTTACGCGCAATATTGATGTTTCAGTGGGtt               <  2:2458989/145‑1 (MQ=255)
                                                                                                                                        tgcttTTTTTATTCCCCGTTTTTCTCGACCGCCAGTATTTAAGTGTGCAAACGCTGACCATGGTTTATAGCAGCGCGCAAATCCTGATCCTGCTGGCAATGGGCGCGACGCTGGTAATGCTTACGCGCAATATTGATGTTTCAGTGGGtt               <  2:2536731/150‑1 (MQ=255)
                                                                                                                                        tgctgTTTGTTTTACCCGCTTTCCGGGGCCGCCAGTATTTAAGTGTGCAAACGCTGACCATGGTTTATAGCAGCGCGCAAATCCTGATCCTGCTGGCAATGGGCGCGACGCTGGTAATGCTTACGCGCAATATTGATGTTTCAGTGGGtt               <  2:3658966/150‑1 (MQ=255)
                                                                                                                                        tgctgTTTGTATTACCGGTTTTTCTCGACCGCCAGTATTTAAGTGTGCAAACGCTGACCATGGTTTATAGCAGCGCGCAAATCCTGATCCTGCTGGCAATGGGCGCGACGCTGGTAATGCTTACGCGCAATATTGATGTTTCAGTGGGtt               <  1:4527500/150‑1 (MQ=255)
                                                                                                                                         gctgTTTGTATTCCCCGGTTTTCTCGACCGCCAGTATTTAAGTGTGCAAACGCTGACCATGGTTTATAGCAGCGCGCAAATCCTGATCCTGCTGGCAATGGGCGCGACGCTGGTAATGCTTACGCGCAATATTGATGTTTCAGTGGGTTc              <  1:3723594/150‑1 (MQ=255)
                                                                                                                                          ctgTTTGTATTACCGGGTTTTCCCGACCGCCAGTATTTAAGTGTGCAAACGCTGACCATGGTTTATAGCAGCGCGCAAATCCTGATCCTGCTGGCAATGGGCGCGACGCTGGTAATGCTTACGCGCAATATTGATGTTTCAGTGGGTTCg             <  1:1187954/150‑1 (MQ=255)
                                                                                                                                          ctgTTTGTATTACCCGGTTTTCTCGACCGCCAGTATTTAAGTGTGCAAACGCTGACCATGGTTTATAGCAGCGCGCAAATCCTGATCCTGCTGGCAATGGGCGCGACGCTGGTAATGCTTACGCGCAATATTGGTGTTTCAGTGGGTTCg             <  1:3149214/150‑1 (MQ=255)
                                                                                                                                           tgTTTGTATTACCCGGTTTTCTCGACCGCCAGTATTTAAGTGTGCAAACGCTGACCATGGTTTATAGCAGCGCGCAAATCCTGATCCTGCTGGCAATGGGCGCGACGCTGGTAATGCTTACGCGCATTATTGATGTTTCAGTGGGTTCGa            >  2:3725377/1‑150 (MQ=255)
                                                                                                                                           tgTTTGTATTACCCGGTTTTCTCGACCGCCAGTATTTAAGTGTGCAAACGCTGACCATGGTTTATAGCAGCGCGCAAATCCTGATCCTGCTGGCAATGGGCGCGACGCTGGTAATGCTTACGCGCAATATTGATGTTTCAGTGGGTTCGa            >  1:3975449/1‑150 (MQ=255)
                                                                                                                                             tttGTATTCCCCGTTTTTCTCGACCGCCAGTATTTAAGTGTGCAAACGCTGACCATGGTTTATAGCAGCGCGCAAATCCTGATCCTGCTGGCAATGGGCGCGACGCTGGTAATGCTTACGCGCAATATTGATGTTTCAGTGGGTTCGAtt          <  1:4719470/150‑1 (MQ=255)
                                                                                                                                             tttGTATTCCCCGGTTTTCTCGACCGCCAGTATTTAAGTGTGCAAACGCTGACCATGGTTTATAGCAGCGCGCAAATCCTGATCCTGCTGGCAATGGGCGCGACGCTGGTAATGCTTACGCGCAATATTGATGTTTCAGTGGGTTCGAtt          <  2:3876617/150‑1 (MQ=255)
                                                                                                                                              ttGTATTACCCGTTTTTCTCGACCGCCAGTATTTAAGTGTGCAAACGCTGACCATGGTTTATAGCAGCGCGCAAATCCTGATCCTGCTGGCAATGGGCGCGACGCTGGTAATGCTTACGCGCAATATTGATGTTTCAGTGGGTTCGATTa         <  2:1807607/150‑1 (MQ=255)
                                                                                                                                              ttGTATTACCCGGTTTTCTCGACCGCCAGTATTTAAGTGTGCAAACGCTGACCATGGTTTATAGCAGCGCGCAAATCCTGATCCTGCTGGCAATGGGCGCGACGCTGGTAATGCTTACGCGCAATATTGATGTTTCAGTGGGTTCGATTa         <  1:651166/150‑1 (MQ=255)
                                                                                                                                              ttGTATTACCCGGTTTTCTCGACCGCCAGTATTTAAGTGTGCAAACGCTGACCATGGTTTATAGCAGCGCGCAAATCCTGATCCTGCTGGCAATGGGCGCGACGCTGGTAATGCTTACGCGCAATATTGATGTTTCAGTGGGTTCGATTa         >  1:4379380/1‑150 (MQ=255)
                                                                                                                                               tGTATTCCCCGGTTTTCTCGACCGCCAGTATTTAAGTGTGCAAACGCTGACCATGGTTTATAGCAGCGCGCAAATCCTGATCCTGCTGGCAATGGGCGCGACGCTGGTAATGCTTACGCGCAATATTGATGTTTCAGTGGGTTCGATTAc        <  2:854569/150‑1 (MQ=255)
                                                                                                                                               tGTATTACCCGGTTTTCTCGACCGCCAGTATTTAAGTGTGCAAACGCTGACCATGGTTTATAGCAGCGCGCAAATCCTGATCCTGCTGGCAATGGGCGCGACGCTGGTAATGCTTACGCGCAATATTGATGTTTCAGTGGGTTCGATTAc        <  1:380472/150‑1 (MQ=255)
                                                                                                                                                     aCCCGGTTTTCTCGACCGCCAGTATTTAAGTGTGCAAACGCTGACCATGGTTTATAGCAGCGCGCAAATCCTGATCCTGCTGGCAATGGGCGCGACGCTGGTAATGCTTACGCGCAATATTGATGTTTCAGTGGGTTCGATTACCGGAAt  >  2:3210446/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CTCTGCACTGACCGAGCGCGATATTAATGTCGAGACTATTATGCGCGTTGCCTTCGGCGATAGTCAGCGTCAGGAGGCGTCATGCTGAAGTTTATTCAGAACAACCGTGAAATCACGGCACTGCTGGCGGTGGTGCTGCTGTTTGTATTACCCGGTTTTCTCGACCGCCAGTATTTAAGTGTGCAAACGCTGACCATGGTTTATAGCAGCGCGCAAATCCTGATCCTGCTGGCAATGGGCGCGACGCTGGTAATGCTTACGCGCAATATTGATGTTTCAGTGGGTTCGATTACCGGAAT  >  AP012306/1309765‑1310063

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑