Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123062,560,2430TG22.3% 525.4 / 18.0 212N419T (AAC→ACC) pepPproline aminopeptidase P II
Reads supporting (aligned to +/- strand):  ref base T (59/105);  new base G (47/0);  total (107/105)
Fisher's exact test for biased strand distribution p-value = 1.89e-17
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 237 total aligned reads displayed.

GGCAGCGCCCCGTGACTTAACCGGGAAATAGCCAGCGCCAGCGTCGCGCCCGCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAATATACAGCCCTGGCTCTACGGTCAGTACCATGCCCGGTTCCAGAATGCGCGAGCGATC  >  AP012306/2560094‑2560391
                                                                                                                                                     |                                                                                                                                                    
 gcggccccccGTGACTTACCGGGAAAATAGCCAGCGCCGGCGTCGCGCCCGCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGtt                                                                                                                                                     <  2:1815392/144‑1 (MQ=255)
  cAGCCCCCGGGGACTAAACGGGAAAAACCCCAGCGCCAGCGCCGCGCCCGCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGttt                                                                                                                                                    <  2:3820839/150‑1 (MQ=255)
   acgcccccGTGATTTAACGGGAAAATGGCCCGCGCCAGCGCCGCGCCCGCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGtttt                                                                                                                                                   <  2:523068/146‑1 (MQ=255)
   aGCGCCCGGTGCCTTAACCGGAAAATACCCAGCGCCAGCGTCGCGCCCGCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGtttt                                                                                                                                                   <  2:483728/150‑1 (MQ=255)
    gcgcCCCGTGACTTAACCGGGAAATAGCCAGCGCCAGCGTCGCGTCCGCCATGCCGCCAACGACGATGATTAAGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTc                                                                                                                                                  <  1:2047745/150‑1 (MQ=255)
    gccccccGTGACTTACCCGGAAAATAGCCAGCGCCAGCGTCGCGCCCGCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTc                                                                                                                                                  <  1:4654982/147‑1 (MQ=255)
      gcCCCGTGACTTAACCGGGAAATAGCCAGCGCCAGCGTCGCGCCCGCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGt                                                                                                                                                >  1:2281215/1‑150 (MQ=255)
       ccccGTGACTTAACCGGGAAATAGCCAGCGCCAGCGTCGCGCCCGCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGtt                                                                                                                                               >  2:4287802/1‑150 (MQ=255)
       ccccGTGACTTAACCGGGAAATAGCCAGCGCCAGCGTCGCGCCCGCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGGTTTCCtt                                                                                                                                               >  2:684714/1‑150 (MQ=255)
          cgtcctttACCCGGAAAATACCCAGCCCCGGCGTCGCGCCCGCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTAcc                                                                                                                                            <  2:1363789/144‑1 (MQ=255)
             gACTTAACCGGGAAATAGCCAGCGCCAGCGTCGCGCCCGCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGt                                                                                                                                         >  1:4068856/1‑150 (MQ=255)
               cTTACCCGGGAAATAGCCAGCGCCGGCGCCGCGCCCGCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGttt                                                                                                                                       <  2:1868002/150‑1 (MQ=255)
                  aaCCGGGAAATAGCCAGCCCCAGCGCCGCCCCCGCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCgg                                                                                                                                    <  2:2514442/150‑1 (MQ=255)
                   aCCGGGAAATAGCCAGCGCCAGCGTCGCGCCCGCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCTGt                                                                                                                                   >  2:3959171/1‑150 (MQ=255)
                      ggaaaaTACCCCGCGCCCGCGGCGCGCCCGCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAAt                                                                                                                                <  2:3179583/147‑1 (MQ=255)
                        gAAATAGCCAGCGCCAGCGTCGCGCCCGCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATca                                                                                                                              >  2:2408426/1‑150 (MQ=255)
                        gAAATAGCCAGCGCCAGCGTCGCGCCCGCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGGTTTCGTTACCGGGTTTGGGAATca                                                                                                                              >  2:3912823/1‑150 (MQ=255)
                          aaTGGCCAGCGCCAGCGTCGCCCCCGCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATcaca                                                                                                                            <  1:2957896/150‑1 (MQ=255)
                          aaTAGCCAGCGCCAGCGTCGCGCCCGCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATcaca                                                                                                                            <  1:2354586/150‑1 (MQ=255)
                            tAGCCAGCGCCAGCGTCGCGCCCGCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGGTTTCGTTACCGGTTTCGGTAATCACAAt                                                                                                                          >  2:2345150/1‑150 (MQ=255)
                             aGCCAGCGCCAGCGTCGCGCCCGCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGGTTCGGTAATCACAATg                                                                                                                         >  2:358515/1‑150 (MQ=255)
                              gccagcgccaggGTCGCGCCCGCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATgt                                                                                                                        <  1:3954668/150‑1 (MQ=255)
                                 agcgccggcgTCGCGCCCGCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATgtcgt                                                                                                                     <  2:1048877/150‑1 (MQ=255)
                                 agcgccggcgTCGCCCCCGCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATgtcgt                                                                                                                     <  2:3422766/150‑1 (MQ=255)
                                 agcgccagcgTCGCGCCCGCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATgtcgt                                                                                                                     <  1:2508222/150‑1 (MQ=255)
                                    gccagcgTCGCGCCCGCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCtt                                                                                                                  >  1:146222/1‑150 (MQ=255)
                                     caagcgTCGCCCCCGCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTc                                                                                                                 <  1:2449766/148‑1 (MQ=255)
                                      cagcgTCGCGCCCGCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCa                                                                                                                >  1:3807162/1‑150 (MQ=255)
                                         cgTCGCGCCCGCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATa                                                                                                             <  1:3072067/150‑1 (MQ=255)
                                            cgcgCCCGCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGa                                                                                                          <  1:1594557/150‑1 (MQ=255)
                                               gcccgccATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATg                                                                                                       <  1:1326165/150‑1 (MQ=255)
                                               gcccgccATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATg                                                                                                       <  2:2925969/150‑1 (MQ=255)
                                               ccccgccATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATg                                                                                                       <  2:877703/149‑1 (MQ=255)
                                                cccgccATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGCGAGGGTTTTGTTACCGGTTTTGGTAATCACAATGTCGGCTTCAATACGAAAGc                                                                                                      >  2:928317/1‑150 (MQ=255)
                                                  cgccATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGCGAGGGTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCg                                                                                                    >  1:4157250/1‑150 (MQ=255)
                                                    ccATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGAt                                                                                                  <  1:1827147/150‑1 (MQ=255)
                                                     cATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATa                                                                                                 >  1:2696228/1‑150 (MQ=255)
                                                      aTGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATAc                                                                                                <  1:4480867/150‑1 (MQ=255)
                                                      aTGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGGTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATAc                                                                                                >  2:1947333/1‑150 (MQ=255)
                                                      aTGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGGTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATAc                                                                                                >  1:1034624/1‑150 (MQ=255)
                                                       tGCCGCCGCCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATAcc                                                                                               <  2:4539043/150‑1 (MQ=255)
                                                        gccgccACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACcg                                                                                              >  1:4340410/1‑150 (MQ=255)
                                                          cgccACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGGTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACcgcg                                                                                            >  1:3621251/1‑150 (MQ=255)
                                                             cACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGGTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGata                                                                                         >  2:4307100/1‑150 (MQ=255)
                                                              aCCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGGTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCCATACGAATGCCGATACCGCGatat                                                                                        >  1:806635/1‑150 (MQ=255)
                                                               ccGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATAtt                                                                                       <  2:755451/150‑1 (MQ=255)
                                                                cgacgaTGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTg                                                                                      <  1:1855680/150‑1 (MQ=255)
                                                                 gacgaTGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGt                                                                                     <  1:374033/150‑1 (MQ=255)
                                                                 gacgaTGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGt                                                                                     <  2:2974826/150‑1 (MQ=255)
                                                                  acgaTGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGtt                                                                                    <  1:1082913/150‑1 (MQ=255)
                                                                    gatgatTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCt                                                                                  <  1:4328488/150‑1 (MQ=255)
                                                                     atgatTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTg                                                                                 <  1:4563932/150‑1 (MQ=255)
                                                                     atgatTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTg                                                                                 <  2:3072901/150‑1 (MQ=255)
                                                                      tgatTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTgg                                                                                >  1:1703217/1‑150 (MQ=255)
                                                                        atTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCa                                                                              >  2:4446971/1‑150 (MQ=255)
                                                                        atTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCa                                                                              <  1:951050/150‑1 (MQ=255)
                                                                        atTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGATTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATTCCGATACCGCGATATTGTTCTCGCa                                                                              >  1:225630/1‑150 (MQ=255)
                                                                           aCGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACtt                                                                           >  1:4384496/1‑150 (MQ=255)
                                                                            cGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTc                                                                          <  2:4632858/150‑1 (MQ=255)
                                                                             gCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCt                                                                         <  1:1614171/150‑1 (MQ=255)
                                                                                cATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGGACTTCTGCa                                                                      >  2:2020586/1‑150 (MQ=255)
                                                                                 aTTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGAGCGGCTTTCCTTACCGTTTTCGGGAATCACAAATTCGTCTTCCATTAGAAAGCCGAAAACGCGATATTTTTCTGGCACTTTTGcct                                                                     >  2:3747548/1‑148 (MQ=255)
                                                                                    gCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCg                                                                  >  1:3787293/1‑150 (MQ=255)
                                                                                     cTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGGTTTCGTTACCCGGTTCGGTAATTACAATTTCGTCTTTAATACGAATGCCGGTACCGCCATATTTTTCTTGCCCTTTTGCCTcccg                                                                 >  2:2054581/1‑148 (MQ=255)
                                                                                        tctcGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGGTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGgcgc                                                              >  2:3235255/1‑150 (MQ=255)
                                                                                           cGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGGTATTGTTCTTGCACTTCTGCATCCCGCGCAat                                                           >  1:3408021/1‑150 (MQ=255)
                                                                                             cagcaACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAatat                                                         <  1:531896/150‑1 (MQ=255)
                                                                                               gcaACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGGTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAATATAc                                                       >  2:1263020/1‑150 (MQ=255)
                                                                                                 aaCCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAATATACAg                                                     <  2:4375848/150‑1 (MQ=255)
                                                                                                 aaCCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGGGAGGGTTTCGTTACCGGTTTCGGTAATCACAATGTTGTCTTCAATACGAATGCCGATACCGCGAAGTTGTTCTGGCACCTCTGCATCCGGCCCAattttcca                                                     >  2:4261365/1‑144 (MQ=255)
                                                                                                  aCCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAATATACAGc                                                    >  1:219467/1‑150 (MQ=255)
                                                                                                  aCCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGGTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAATATACAGc                                                    >  1:337983/1‑150 (MQ=255)
                                                                                                   ccATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAATATACAGcc                                                   <  1:987621/150‑1 (MQ=255)
                                                                                                    cATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGGTTTCGTTACCGGGTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAATATACAGccc                                                  >  1:2731525/1‑150 (MQ=255)
                                                                                                     aTCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTTTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAATATACAGCCCt                                                 <  2:2181674/150‑1 (MQ=255)
                                                                                                       cAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGGTTTCGTTACCGGGTTCGGTAATCACAATGGCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAATATACAGCCCTgg                                               >  1:604447/1‑150 (MQ=255)
                                                                                                        aaCGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGGTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAATATACAGCCCTGGc                                              >  1:1400552/1‑150 (MQ=255)
                                                                                                         aCGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAATATACAGCCCTGGct                                             <  1:2679648/150‑1 (MQ=255)
                                                                                                          cGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAATATACAGCCCTGGctc                                            <  2:1689688/150‑1 (MQ=255)
                                                                                                             ttCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGGTTTCGTTTCCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAATATACAGCCCTGGCTCTAc                                         >  2:4091050/1‑150 (MQ=255)
                                                                                                                  tttCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTc                                                                          >  2:3354435/1‑112 (MQ=255)
                                                                                                                       tcCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAATATACAGCCCTGGCTCTACGGTCAGTAcc                               >  1:3301283/1‑150 (MQ=255)
                                                                                                                       tcCGGCTTTTTCACCACGCTGGCGGTGAGGGTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAATATACAGCCCTGGCTCTACGGTCAGTAcc                               >  1:2871943/1‑150 (MQ=255)
                                                                                                                          ggCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCCATATACAGCCCTGGCTCTACGGTCAGTACCATg                            >  1:2914823/1‑150 (MQ=255)
                                                                                                                          ggCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAATATACAGCCCTGGCTCTACGGTCAGTACCATg                            >  2:1549748/1‑150 (MQ=255)
                                                                                                                               tttCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAATATACAGCCCTGGCTCTACGGTCAGTACCATGCCCgg                       <  1:3914269/150‑1 (MQ=255)
                                                                                                                                ttCACCACGCTGGCGGTGAGGTTTTCGTTACTGGTTTCGGTAATCACAATGTCGTCTTTAATACGAATGCCGATACCGCGATATTTTTCTGGCACTTCTGCATCCGGCGCAATATACAGCCCCGGCTCTACGGTCAGTACCATGCCCGGt                      >  2:1497981/1‑150 (MQ=255)
                                                                                                                                  caccacGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAAAGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAATATACAGCCCTGGCTCTACGGTCAGTACCATGCCCGGTTc                    >  2:3450558/1‑150 (MQ=255)
                                                                                                                                   accacGCTGGCGGTGAGGGTTTCGTTACCGGTTTCGGTAATCACAATGTCGGCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAATATACAGCCCTGGCTCTACGGTCAGTACCATGGCCGGTTcc                   >  2:2691060/1‑150 (MQ=255)
                                                                                                                                       cGCTGGCGGTGAGGGTTTCGTTACCCGGTTCGGGAATCACAATGTCGTCTTCAATACGAATGCCGATAACGCGATATTGTTCTGGCACTTCTGCATCCCGCGCAATATACAACCCCGGCTCTACGGGAAGTACCCAGCCCGGTTCCAGaa               >  2:1075810/1‑150 (MQ=255)
                                                                                                                                           ggCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAATATACAGCCCTGGCTCTACGGTCAGTACCATGCCCGGTTCCAGAATgcg           >  2:1304625/1‑150 (MQ=255)
                                                                                                                                             cGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAATATACAGCCCTGGCTCTACGGTCAGTACCATGCCCGGTTCCAGAATGcgcg         >  1:2141245/1‑150 (MQ=255)
                                                                                                                                              ggTGAGGTTTTCGTTACCGGTTTCGGTAATCCCAATGTCGTCTTCACTACGAATGGCGGTACCGCGATATTGTGCTAGCACTTCTCCATCCGGCGCAATATCCAGCGCTGTCTCTACGGACAGCACCATGCCCGGTTCGCTAATgcgcta        >  2:2650047/1‑148 (MQ=255)
                                                                                                                                               gTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAATATACAGCCCTGGCTCTACGGTCAGTACCATGCCCGGTTCCAGAATGCgcgag       <  2:1194788/150‑1 (MQ=255)
                                                                                                                                                tGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAATATACAGCCCTGGCTCTACGGTCAGTACCATGCCCGGTTCCAGAATGCgcgagc      >  1:3175282/1‑150 (MQ=255)
                                                                                                                                                   ggTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAATATACAGCCCTGGCTCTACGGTCAGTACCATGCCCGGTTCCAGAATGCGCGAGCGAt   >  2:768079/1‑150 (MQ=255)
                                                                                                                                                   ggTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGACGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAATATACAGCCCTTGCTCTACGGTCAGTACCATGCCCGGTTCCAGAATGCGCGAGCGAt   >  2:2443326/1‑150 (MQ=255)
                                                                                                                                                    gTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAATATACAGCCCTGGCTCTACGGTCAGTACCATGCCCGGTTCCAGAATGCGCGAGCGATc  <  2:223981/150‑1 (MQ=255)
                                                                                                                                                    gTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAATATACAGCCCTGGCTCTACGGTCAGTACCATGCCCGGTTCCAGAATGCGCGAGCGATc  >  1:1281780/1‑150 (MQ=255)
                                                                                                                                                    gTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAATATACAGCCCTGGCTCTACGGTCAGTACCATGCCCGGTTCCAGAATGCGCGAGCGATc  >  2:949279/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
GGCAGCGCCCCGTGACTTAACCGGGAAATAGCCAGCGCCAGCGTCGCGCCCGCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAATATACAGCCCTGGCTCTACGGTCAGTACCATGCCCGGTTCCAGAATGCGCGAGCGATC  >  AP012306/2560094‑2560391

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑