Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123063,020,6700TG32.8% 339.9 / 18.3 189K195N (AAA→AAC) glgCglucose‑1‑phosphate adenylyltransferase
Reads supporting (aligned to +/- strand):  ref base T (77/50);  new base G (62/0);  total (139/50)
Fisher's exact test for biased strand distribution p-value = 5.95e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 258 total aligned reads displayed.

ATCAAATCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGC‑AGGTTTTTC‑AACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGATAAGCATACGCGAGTAGTCTTGCTT  >  AP012306/3020521‑3020819
                                                                                                                                                      |                                                                                                                                                      
aTCAAATCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGC‑AGGt                                                                                                                                                        <  2:1671019/150‑1 (MQ=255)
  cAAATCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGC‑AGGttt                                                                                                                                                      <  2:2681043/150‑1 (MQ=255)
   aaaTCTTTCCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGC‑AGGtttt                                                                                                                                                     <  2:1877252/150‑1 (MQ=255)
    aaTCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGC‑AGGttttt                                                                                                                                                    <  2:3821117/150‑1 (MQ=255)
    aaTCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGGTAGC‑AGGgtttt                                                                                                                                                    >  1:1586898/1‑150 (MQ=255)
     aTCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGC‑AGGTTTTTc                                                                                                                                                   <  1:1044910/150‑1 (MQ=255)
       cTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGGTAGC‑AGGGTTTTC‑aa                                                                                                                                                >  1:1270171/1‑150 (MQ=255)
       cTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGGTAGC‑AGGGGTTTC‑aa                                                                                                                                                >  2:194491/1‑150 (MQ=255)
         ttGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGC‑AGGTTTTTC‑AACg                                                                                                                                              <  1:990478/150‑1 (MQ=255)
           gCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGC‑AGGTTTTTC‑AACGaa                                                                                                                                            <  2:1122605/150‑1 (MQ=255)
             cAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGC‑AGGTTTTTC‑AACGAAtt                                                                                                                                          <  2:3565206/150‑1 (MQ=255)
              aaaGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAAGGTTTTTA‑ACCAAAtt                                                                                                                                          >  2:4731891/1‑150 (MQ=255)
              aaaGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGC‑AGGGTTTTT‑TACGaacta                                                                                                                                         >  2:3281470/1‑147 (MQ=255)
              aaaGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGC‑AGGGTTTTC‑AACGAATTc                                                                                                                                         >  1:3836022/1‑150 (MQ=255)
                aGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGGTAGC‑AGGGTTTTC‑AACGAATTCGa                                                                                                                                       >  1:573311/1‑150 (MQ=255)
                  tCGTGGCTTAAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGC‑AGGTTTTTC‑AACGAATTCGATa                                                                                                                                     <  2:3886930/150‑1 (MQ=255)
                  tCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGC‑AGGTTTTTC‑AACGAATTCGATa                                                                                                                                     <  1:852379/150‑1 (MQ=255)
                   cGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGC‑AGGTTTTTC‑AACGAATTCGATaa                                                                                                                                    <  1:4198207/150‑1 (MQ=255)
                     tGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGC‑AGGGTTTTT‑AACCAATTCGATAAtt                                                                                                                                  >  2:3503209/1‑150 (MQ=255)
                       gCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGC‑AGGTTTTTC‑AACGAATTCGATAAtttt                                                                                                                                <  1:439643/150‑1 (MQ=255)
                       gCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGC‑AGGGTTTTT‑AACGAATTCGATAAtttt                                                                                                                                >  2:4078206/1‑150 (MQ=255)
                        cTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGC‑AGGTTTTTC‑AACGAATTCGATAATTTTa                                                                                                                               <  2:2365819/150‑1 (MQ=255)
                         tGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGC‑AGGTTTTTC‑AACGAATTCGATAATTTTAt                                                                                                                              <  2:195391/150‑1 (MQ=255)
                          ggAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGGTAGC‑AGGTTTTTC‑AACGAATTCGATAATTTTTTc                                                                                                                             >  1:1157285/1‑150 (MQ=255)
                           gAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGC‑AGGTTTTTC‑AACGAATTCGATAATTTTATCg                                                                                                                            <  2:1159820/150‑1 (MQ=255)
                             gTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGATTGC‑CGGTTTTTT‑AACGAAATCGATAATTTTATCGtt                                                                                                                          >  2:3678516/1‑150 (MQ=255)
                               tctcATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATGCCCATGCTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGC‑AGGTTTTTC‑AACGAATTCGATAATTTTATCGTtct                                                                                                                        <  2:1641593/150‑1 (MQ=255)
                               tctcATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGC‑AGGTTTTTC‑AACGAATTCGATAATTTTATCGTtct                                                                                                                        <  1:3874419/150‑1 (MQ=255)
                                ctcATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGC‑AGGTTTTTC‑AACGAATTCGATAATTTTATCGTtctc                                                                                                                       <  2:1397558/150‑1 (MQ=255)
                                 tcATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGC‑AGGTTTTTC‑AACGAATTCGATAATTTTATCGTTCtca                                                                                                                      <  2:1850235/150‑1 (MQ=255)
                                 tcATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGC‑AGGTTTTTC‑AACGAATTCGATAATTTTATCGTTCtca                                                                                                                      <  2:4616357/150‑1 (MQ=255)
                                  cATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGC‑AGGTTTTTC‑AACGAATTCGATAATTTTATCGTTCtcat                                                                                                                     <  1:2303529/150‑1 (MQ=255)
                                  cATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGC‑AGGTTTTTC‑AACGAATTCGATAATTTTATCGTTCtcat                                                                                                                     <  2:2220367/150‑1 (MQ=255)
                                       cgATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGC‑AGGTTTTTC‑AACGAATTCGATAATTTTATCGTTCTCATCAAcc                                                                                                                <  2:4312677/150‑1 (MQ=255)
                                       cgATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGC‑AGGGTTTTC‑AACGAATTCGATAATTTTATCGTTCTCATCAAcc                                                                                                                >  1:769327/1‑150 (MQ=255)
                                        gATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGC‑AGGTTTTTC‑AACGAATTCGATAATTTTATCGTTCTCATCAACCg                                                                                                               >  2:1278075/1‑150 (MQ=255)
                                        gATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGC‑AGGTTTTTC‑AACGAATTCGATAATTTTATCGTTCTCATCAACCg                                                                                                               <  1:867096/150‑1 (MQ=255)
                                        gATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGC‑AGGTTTTTC‑AACGAATTCGATAATTTTATCGTTCTCATCAACCg                                                                                                               <  2:4474084/150‑1 (MQ=255)
                                         aTCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGGTAGC‑AGGGTTTTT‑AACGAATTCGAAAAATTTATCTTTTtcattcaacgc                                                                                                              >  2:925085/1‑143 (MQ=255)
                                            gTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGC‑AGGTTTTTC‑AACGAATTCGATAATTTTATCGTTCTCATCAACCGCCAt                                                                                                           >  1:4347373/1‑150 (MQ=255)
                                            gTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGGTAGC‑AGGGTTTTC‑AACGAATTCGATAATTTTATCGTTCTCATCAACCGccct                                                                                                           >  2:451766/1‑148 (MQ=255)
                                               ttcttcCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGC‑AGGTTTTTC‑AACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAAc                                                                                                        >  2:3065318/1‑150 (MQ=255)
                                                 cttcCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGGTAGC‑AGGTTTTTC‑AACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGc                                                                                                      >  2:2688000/1‑150 (MQ=255)
                                                  ttcCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGC‑AGGTTTTTC‑AACGAATTCGATAATTTTATCGTTCTCCTCAACCGCCATAACGcc                                                                                                     >  1:4540803/1‑150 (MQ=255)
                                                    ccAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGC‑AGGTTTTTC‑AACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCaa                                                                                                   >  1:3688069/1‑150 (MQ=255)
                                                       gcagTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGC‑AGGTTTTTT‑TACAAATTCGATAATTTTATTGGTGTCATCTACCCCCATAACACCCAATg                                                                                                >  2:3011888/1‑150 (MQ=255)
                                                       gcagTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGC‑AGGTTTTTC‑AACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATg                                                                                                >  1:427930/1‑150 (MQ=255)
                                                       gcagTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGC‑AGGGTTTTC‑AACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATg                                                                                                >  2:885404/1‑150 (MQ=255)
                                                        cagTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGC‑AGGTTTTTC‑AACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGc                                                                                               >  1:2951665/1‑150 (MQ=255)
                                                               tACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGC‑AGGGTTTTC‑AACGAATTCGATAATTTTATCGGTTTCATCAACCGCCATAACGCCCAATGCGGAGGct                                                                                        >  2:1904136/1‑150 (MQ=255)
                                                                aCAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGC‑AGGTTTTTC‑AACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGGGGctt                                                                                       >  2:3653768/1‑150 (MQ=255)
                                                                aCAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGC‑AGGTTTTTC‑AACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGctt                                                                                       >  1:2707664/1‑150 (MQ=255)
                                                                  aGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGC‑AGGGTTTTT‑AACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGcttct                                                                                     >  2:4028358/1‑150 (MQ=255)
                                                                   gATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGGTAGC‑AGGGTTTTC‑AACGAATTCGATAATTTTATCGTTCTCATCAAACGCCATAACGCCAAATGCGGAGGcttctt                                                                                    >  2:4569523/1‑150 (MQ=255)
                                                                    aTAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGC‑AGGTTTTTC‑AACGAATTCGATAATTTTTTCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGcttcttt                                                                                   >  2:4250458/1‑149 (MQ=255)
                                                                    aTAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGC‑AGGGTTTTC‑AACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCttcttc                                                                                   >  2:1736939/1‑150 (MQ=255)
                                                                    aTAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGATAGG‑AGGGTTTTT‑AAACAATTTGATAATTTTTTTGTTTTCCTCAAACGCCATAACACCAAATTAGGGGGgttcttt                                                                                   >  2:2266484/1‑149 (MQ=255)
                                                                     tAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGC‑AGGTTTTTT‑AAAGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGGGGCTTTTTCa                                                                                  >  2:3588359/1‑150 (MQ=255)
                                                                     tAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGC‑AGGGTTTTC‑AACGAATTCGATAATTTTATCGTTCTCATCAACCGCCCTAACGCCAAATGCGGAGGCTTCTTCa                                                                                  >  1:3534352/1‑150 (MQ=255)
                                                                      aGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGGTAGG‑AGGGTTTTT‑AACGAAATCGGTAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGGTTCTTCaa                                                                                 >  2:4156452/1‑150 (MQ=255)
                                                                       gTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGC‑AGGTTTTTC‑AACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAAt                                                                                >  1:3426871/1‑150 (MQ=255)
                                                                       gTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGC‑AGGTTTTTC‑AACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAAt                                                                                >  1:1900852/1‑150 (MQ=255)
                                                                       gTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGC‑AGGGTTTTC‑AACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAAt                                                                                >  1:3968419/1‑150 (MQ=255)
                                                                          ggCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGC‑AGGTTTTTC‑AACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCgg                                                                             >  1:1516296/1‑150 (MQ=255)
                                                                            cGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGC‑AGGGTTTTC‑AACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTa                                                                           >  2:626537/1‑150 (MQ=255)
                                                                            cGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGGTAGC‑AGGTTTTTC‑AACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTa                                                                           >  1:1671205/1‑150 (MQ=255)
                                                                                  aGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGC‑AGGGTTTTC‑AACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCCAATGCGGAGGCTTCTTTAATCGGTACTGGCa                                                                     >  2:2617120/1‑150 (MQ=255)
                                                                                     cGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGC‑AGGTTTTTC‑AACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATAc                                                                  >  2:1611247/1‑150 (MQ=255)
                                                                                      gTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGC‑AGGGTTTTC‑AACGAATTCGATAATTTTATCGTTCTCATCAACCCCCATAACGCCAAATGCGGAGGCTTCTTCCATCGGTACTGGCATACa                                                                 >  1:1966591/1‑150 (MQ=255)
                                                                                        aaacccccATCCCCGCAAGGGTTTTGCTGGGATCTTTGGGCTTTGGGGGGGGGTTGGC‑GTTTTTTAA‑AAAAAATTAAATATTTTTCTCTTTCACAACACCCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAg                                                               <  2:3518596/145‑1 (MQ=255)
                                                                                        aGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGC‑AGGTTTTTC‑AACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAg                                                               >  1:1190039/1‑150 (MQ=255)
                                                                                        aGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGC‑AGGGTTTTC‑AACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAg                                                               >  1:4606095/1‑150 (MQ=255)
                                                                                            aCCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGC‑AGGGTTTTC‑AACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAAc                                                           >  1:312458/1‑150 (MQ=255)
                                                                                                      accAGAATTTTGCCCGGCCCGCTCGGATTTGAGGGGGGGTGAGA‑AGTTTTTTA‑AACAAATTCGTTATTTTTCTCTTTCTCAAAAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGt                                                 <  2:1770063/149‑1 (MQ=255)
                                                                                                        cAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGC‑AGGGTTTTT‑AACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCCGTACTGGCATACAAGCAACGGTGCAACGTgc                                               >  1:1399827/1‑150 (MQ=255)
                                                                                                             aTTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGC‑AGGTTTTTC‑AACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCtt                                          >  1:490776/1‑150 (MQ=255)
                                                                                                              tttGCTCGGATCGTTCGGCATTGACGGCGGGTTAGC‑AGGTTTTTT‑AACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCttt                                         >  2:3457359/1‑150 (MQ=255)
                                                                                                                tGCTCGGATCGTTCGGCATTGACGGCGGGTTAGC‑AGGGTTTTC‑AACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCttttt                                       >  2:3169223/1‑150 (MQ=255)
                                                                                                                 gCTCGGATCGTTCGGCATTGACGGCGGGGTAGC‑AGGTTTTTC‑AACCAATTCGATAATTTTTTTGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGGTTCTTCAATCGGTACTTGCATACAAGCAACGGCGCCACGTGCGCCCTTTTc                                      >  2:864216/1‑150 (MQ=255)
                                                                                                                            tCGGCATTGACGGCGGGTTAGC‑AGGTTTTTC‑AACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGa                           >  1:332177/1‑150 (MQ=255)
                                                                                                                              ggCATTGACGGCGGGTTAGC‑AGGTTTTTC‑AACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGATa                         >  2:217447/1‑150 (MQ=255)
                                                                                                                                cATTGACGGCGGGTTAGC‑AGGTTTTTC‑AACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGATAAg                       >  1:1060568/1‑150 (MQ=255)
                                                                                                                                cATTGACGGCGGGTTAGC‑AGGTTTTTC‑AACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGATAAg                       >  1:4568652/1‑150 (MQ=255)
                                                                                                                                cATTGACGGCGGGTTAGC‑AGGGTTTTC‑AACGAATTCGATAATTTTTTCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAACAACGGGGCAACGTGCGCCTTTTTCGACGTGGTCGATAAg                       >  2:63406/1‑150 (MQ=255)
                                                                                                                                 aTTGACGGCGGGTTAGC‑AGGTTTTTC‑AACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGATAAGc                      >  1:1923079/1‑150 (MQ=255)
                                                                                                                                    gagggggggTTAGC‑AGTTTTTTA‑AACGAATTAGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGATAAGCATa                   <  1:697168/144‑1 (MQ=255)
                                                                                                                                       ggcggGTTAGC‑TTTTTTTTC‑AACAAATTCTATATTTTTTTCTTTCTCACCACCCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGATAAGCATAcgc                <  2:1471406/150‑1 (MQ=255)
                                                                                                                                       ggcggGTTAGC‑AGGTTTTTC‑AACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGATAAGCATAcgc                >  1:179641/1‑150 (MQ=255)
                                                                                                                                       ggcggGTTAGC‑AGGTTTTTC‑AACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGATAAGCATAcgc                >  1:213158/1‑150 (MQ=255)
                                                                                                                                         ggggTTAGT‑AGTTTTTTA‑AACAAATTCATTATTTTTTTCTTCCTCAACACCCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGATAAGCATACGCGa              <  2:303930/149‑1 (MQ=255)
                                                                                                                                           ggTTAGC‑AGGTTTTTC‑AACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGCTCGATAAGCATACGCGagt            >  1:3461781/1‑150 (MQ=255)
                                                                                                                                           ggTTAGA‑AGTTTTTAA‑AACGAATTCGATATTTTTATCTTTCTAACCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGATAAGCATACGCGagt            <  1:265611/150‑1 (MQ=255)
                                                                                                                                            gTTAGC‑GGTTTTTAC‑AACGATTCCGATATTTTAATCGTCCCCACCACCCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGATAAGCATACGCGagta           <  1:2256987/150‑1 (MQ=255)
                                                                                                                                            gTTAGC‑AGGTTTTTC‑AACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGATAAGCATACGCGagta           >  2:3940533/1‑150 (MQ=255)
                                                                                                                                                gC‑AGGTTTTTC‑AACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGATAAGCATACGCGAGTAGTct       >  1:3200122/1‑150 (MQ=255)
                                                                                                                                                gC‑AGGTTTTTC‑AACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGATAAGCATACGCGAGTAGTct       >  1:3309649/1‑150 (MQ=255)
                                                                                                                                                gC‑AGGTTTTTC‑AACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGATAAGCATACGCGAGTAGTct       >  2:3404439/1‑150 (MQ=255)
                                                                                                                                                 c‑AGGTTTTTC‑AACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGATAAGCATACGCGAGTAGTctt      >  2:2889792/1‑150 (MQ=255)
                                                                                                                                                    attttttCAAACAAATTCGATATTTTTATCTTTCTCACCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGATAAGCATACGCGAGTAGTcttg     <  2:2069291/148‑1 (MQ=255)
                                                                                                                                                     gTTTTTC‑AACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGATAAGCATACGCGAGTAGTcttgct   >  1:3745747/1‑150 (MQ=255)
                                                                                                                                                      |                                                                                                                                                      
ATCAAATCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGC‑AGGTTTTTC‑AACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGATAAGCATACGCGAGTAGTCTTGCTT  >  AP012306/3020521‑3020819

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑