Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123063,903,7910AG23.0% 661.1 / 78.6 278E189G (GAA→GGA) yjgJpredicted transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (33/181);  new base G (64/0);  total (97/181)
Fisher's exact test for biased strand distribution p-value = 1.11e-38
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 396 total aligned reads displayed.

GACCATTTATGACTACATCGCCAGGCGGCATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCC‑GAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTA‑GCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAACGTT  >  AP012306/3903642‑3903940
                                                                                                                                                     |                                                                                                                                                       
  ccATTTATGACTACATCGCCAGGCGGCATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAg                                                                                                                                                       <  2:1894698/150‑1 (MQ=255)
   cATTTATGACTACATCGCCAGGCGGCATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGCAgg                                                                                                                                                      >  1:4118988/1‑149 (MQ=255)
   cATTTATGACTACATCGCCAGGCGGCATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGc                                                                                                                                                      >  1:2844/1‑150 (MQ=255)
    aTTTATGACTACATCGCCAGGCGGCATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGCg                                                                                                                                                     >  1:3537616/1‑150 (MQ=255)
    aTTTATGACTACATCGCCAGGCGGCATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGCg                                                                                                                                                     >  1:4244162/1‑150 (MQ=255)
    aTTTATGACTACATCGCCAGGCGGCATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCg                                                                                                                                                     <  2:3387008/150‑1 (MQ=255)
     tttATGACTACATCGCCAGGCGGCATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGgagaga                                                                                                                                                    >  1:2675517/1‑150 (MQ=255)
       tATGACTACATCGCCAGGCGGCATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATa                                                                                                                                                  <  2:471426/150‑1 (MQ=255)
        aTGACTACATCGCCAGGCGGCATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGATaa                                                                                                                                                 >  1:3182069/1‑150 (MQ=255)
         tGACTACATCGTCAGGCGGCATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTCCCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATaaa                                                                                                                                                <  1:3831668/150‑1 (MQ=255)
         tGACTACATCGCCAGGCGGCATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGGTaaa                                                                                                                                                >  2:2812369/1‑150 (MQ=255)
           aCTACATCGCCAGGCGGCATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATaaaaa                                                                                                                                              <  1:4052246/150‑1 (MQ=255)
           aCTACATCGCCAGGCGGCATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATaaaaa                                                                                                                                              >  1:2801074/1‑150 (MQ=255)
             tACATCGCCAGGCGGCATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGATaaaaaat                                                                                                                                            >  2:1276010/1‑148 (MQ=255)
                  cGCCAGGCGGCATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCt                                                                                                                                       <  2:2044831/150‑1 (MQ=255)
                   gCCAGGCGGCATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCtt                                                                                                                                      <  1:4643781/150‑1 (MQ=255)
                   gCCAGGCGGCATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCtt                                                                                                                                      >  1:3117015/1‑150 (MQ=255)
                    ccAGGCGGCATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTg                                                                                                                                     <  1:133277/150‑1 (MQ=255)
                       ggcggcATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGCAGGGAAAAAAACTATTTTTgtgg                                                                                                                                  >  1:691891/1‑150 (MQ=255)
                         cggcATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTtaggag                                                                                                                                >  1:2264324/1‑150 (MQ=255)
                         cggcATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTgaggag                                                                                                                                <  1:3598910/150‑1 (MQ=255)
                          ggcATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGGTAAAAACTATtttttgggggg                                                                                                                               >  2:4086614/1‑143 (MQ=255)
                            cATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGGGAGAAAAACTTTTCTTgtgggggca                                                                                                                             >  2:1645519/1‑145 (MQ=255)
                             aTCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGATAAAAAATATTTTTTTGGAGTGAt                                                                                                                            >  2:3449286/1‑150 (MQ=255)
                              tCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGGGATAAAAAATATTTTTTAGGGGTGATg                                                                                                                           >  2:2171337/1‑150 (MQ=255)
                              tCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGACAAAAACTATTCTTTATGAGGGATg                                                                                                                           >  2:1517286/1‑150 (MQ=255)
                                cacaACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGTGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCg                                                                                                                         <  2:4697314/150‑1 (MQ=255)
                                  caACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAACGGGAAAAACTATTCTTTAGGAGGGGAGCggg                                                                                                                       >  2:1852721/1‑150 (MQ=255)
                                   aaCGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGGGGTAAAAAAAATTTTTTAGGAGGGGAGCgggg                                                                                                                      >  2:296350/1‑149 (MQ=255)
                                    aCGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTTAGGAGTGATGCGGGcc                                                                                                                     >  1:1049358/1‑150 (MQ=255)
                                     cgcgCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCt                                                                                                                    <  1:4330202/150‑1 (MQ=255)
                                     cgcgCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCt                                                                                                                    <  1:2392217/150‑1 (MQ=255)
                                           caATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGAccc                                                                                                              <  2:2077312/150‑1 (MQ=255)
                                            aaTGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGATAAAAAATATTTTTGAGGAGTGATGCGGGGCTTGACCC‑g                                                                                                            >  2:460549/1‑150 (MQ=255)
                                            aaTGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCC‑g                                                                                                            >  1:2746207/1‑150 (MQ=255)
                                             aTGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGATAAAAAATATTTTTTTGGAGAGATGCGGGCCTTGTCCC‑Ga                                                                                                           >  2:1454477/1‑150 (MQ=255)
                                             aTGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGATAAAAAATATTCTTTAGGAGTGATGCGGGCCTTGACCC‑Ga                                                                                                           >  2:4535231/1‑150 (MQ=255)
                                              tgtgtgACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGGGATGCGGGCCTTGACCC‑Gaa                                                                                                          >  1:4487824/1‑150 (MQ=255)
                                                   gACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGATAAAAACAATTCTTTAGGAGGGATGCGGGCCCTGGCCC‑GAAAAGCg                                                                                                     >  2:2417290/1‑150 (MQ=255)
                                                       gATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGGTAAAAAATATTCTTTAGGAGGGATGCGGGCCTTGACCC‑GAAAGGCgggga                                                                                                 >  1:2869581/1‑148 (MQ=255)
                                                         ttttATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGATAAAAAATATTCTTGAGGAGGGATGCGGGCCTTTGCCC‑GAAAAGCGGGGAAg                                                                                               >  1:3879497/1‑150 (MQ=255)
                                                          tttATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCC‑GAAAGGCGGGAAAgc                                                                                              <  2:929929/150‑1 (MQ=255)
                                                            tATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCC‑GAAAGGCGGGAAAgcgc                                                                                            <  1:3351741/150‑1 (MQ=255)
                                                            tATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCC‑GAAAGGCGGGAAAgcgc                                                                                            <  2:2624233/150‑1 (MQ=255)
                                                             aTGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGAGGTAAAAAATATTTTTTAGGAGGGAGGGGGGGCCTTACCC‑GCAAGGCGGGAAAGCGcc                                                                                           >  2:2364552/1‑150 (MQ=255)
                                                              tGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCC‑GAAAGGCGGGAAAGCGCCt                                                                                          <  1:2530105/150‑1 (MQ=255)
                                                              tGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAAGGGGAAAAACAATTTTTTAGGAGTGGTTGGGGCCTTGGCCC‑GAAAGGCGGGGAAgaggcc                                                                                          >  2:355048/1‑145 (MQ=255)
                                                                aGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGATAAAAAATATTCTTGAGGAGTGATGCGGGCCTTGACCC‑GAAAGGCGGGGAAGCGCCTGc                                                                                        >  2:3030112/1‑150 (MQ=255)
                                                                aGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCC‑GAAAGGCGGGAAAGCGCCTGc                                                                                        <  2:1171465/150‑1 (MQ=255)
                                                                  tACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGGCAAAAAAAATTCTTGTGGAGGGGTGCGGGGCTTGAGCC‑GAAAGGGGGGGAAAAGCCTGCCg                                                                                      >  2:4214254/1‑150 (MQ=255)
                                                                  tACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAAATATTTTTGAGGAGTGATGCGGGGCTTTACCC‑CAAAGGCGGGGAAGCGCccgccg                                                                                      >  1:1102195/1‑150 (MQ=255)
                                                                      gTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCC‑GAAAGGCGGGAAAGCGCCTGCCGATaa                                                                                  <  1:527468/150‑1 (MQ=255)
                                                                        gATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGATAAAAAATATTCTTTGGGAGGGATGGGGGCCTTTGCCC‑GAAAGGCGGGGAAAGGCCTGGCGaataag                                                                                >  2:4343199/1‑145 (MQ=255)
                                                                             cTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCC‑GAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGa                                                                           >  1:832574/1‑150 (MQ=255)
                                                                                ggCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCCGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCC‑GAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATaa                                                                        <  1:4431104/150‑1 (MQ=255)
                                                                                 gCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGATAAAAAATATTCTTGAGGGGTGGTGCGGGCCTTGACCC‑CCAAGGCGGGGAAGAGCCCGCCCGTAAATTGTGGTAAg                                                                       >  2:3479895/1‑150 (MQ=255)
                                                                                 gCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCC‑GAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAg                                                                       <  1:756392/150‑1 (MQ=255)
                                                                                  cTTCCTGCGAAGGCACGAGAGAGGCACTCTATAGAACACCTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGCCCC‑GAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGa                                                                      <  2:1801986/150‑1 (MQ=255)
                                                                                   tttCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCC‑GAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGAc                                                                     <  1:916216/150‑1 (MQ=255)
                                                                                        gCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCC‑GAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACaataa                                                                <  1:2060012/150‑1 (MQ=255)
                                                                                         cGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAACGGGAAAAAAAATTCTTGTGGAGGGATGCGGGGCCTGACCC‑CAAAAGGGGGGAAGAGCCTACCGAGAAGTTGTGGAAAGACaaaaaa                                                               >  2:3149436/1‑149 (MQ=255)
                                                                                         cGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCC‑GAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACaataat                                                               <  1:1250001/150‑1 (MQ=255)
                                                                                           aaGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGATAAAAAAAATTCTTGAGGGGGGATGCGGGGCTCGCCCCGGAAAGCGGGGAAACCGCC‑GCCGGTAAAATGTGATAAGACAAAAAATc                                                             >  2:3115830/1‑150 (MQ=255)
                                                                                               cACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGGGATAAAAAATATTCTTGAGGAGGGATGCGGGCCTTGACCC‑CAAAGGGGGGGAAACGCCCGCCGGTAAAGTGTGGTAAAAAAAAAAATCaacg                                                         >  2:1384049/1‑147 (MQ=255)
                                                                                                  gagaGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCC‑GAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGtt                                                      <  1:4549609/150‑1 (MQ=255)
                                                                                                        ggCACTTTATAAAACAACTCTGTGAAACAGCTGCAATGGCGGGGGAGGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCTTTGACCC‑GAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCt                                                <  2:2285488/150‑1 (MQ=255)
                                                                                                        ggCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCC‑GAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCt                                                <  1:1724963/150‑1 (MQ=255)
                                                                                                           aCTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGATAAAAAATATTCTTGAGGAGTGATGCGGGGCTTGACCC‑GAAAGGCGGGGAAGAGCCTGCCGATAAGATGTGATAAGACAATAATTTACGCGTTAAGGCTA‑Gc                                            >  2:4017693/1‑150 (MQ=255)
                                                                                                            ctctATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGATAAAAAATATTTTTGAGGAGTGATGCGGGGCTTGACCC‑GAAAGGCGGGGAAGAGCCTGCCGATAAGTTGTGATAAGACAATAAATTACGCGTTAAGGCTA‑GCg                                           >  2:4272938/1‑150 (MQ=255)
                                                                                                            ctctATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGATAAAAAAAATTTTTGAGGAGGGGTGCGGGCCTTGACCC‑GAAAAGGGGGGAAGCGCCCGCCGGTAAGGTTTGATAAGAAAAAAAATCACGCGGTAAGGGTA‑GCg                                           >  2:578058/1‑150 (MQ=255)
                                                                                                              ctATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGATAAAAAATATTTTTTAGGAGGGATGCGGGGCCTGACCC‑CAAAGGCGGGGAAACGCCCGCCGGTAAAGTGTGGTAAAACAAAAATTTACGCGGTAAAGCTA‑ACGGa                                         >  2:1574437/1‑150 (MQ=255)
                                                                                                               tataGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGATAAAAAATATTTTTTAGGAGGGATGCGGGCCTTGACCC‑GAAAAGCGGGGAAACGCCTGCCGGTAAGGTGTGATAAAACAAAAAATTACGCGGTAAAGGTA‑GCGGaa                                        >  2:4654410/1‑150 (MQ=255)
                                                                                                               tataGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCC‑GAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTA‑GCGGaa                                        <  2:3251648/150‑1 (MQ=255)
                                                                                                                 taGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCC‑GAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTA‑GCGGAAtt                                      <  2:64667/150‑1 (MQ=255)
                                                                                                                  aGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGATAAAAAATATTCTTTAGGAGTGATGCGGGGCTTGACCC‑GAAAAGCGGGGAAACGCCTGCCGGTAAGTTGTGATAAAACAAAAAATTACCCGTTAAAGCTA‑GCGGAAATg                                     >  2:496924/1‑150 (MQ=255)
                                                                                                                   gAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCC‑GAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTA‑GCGGAATTGa                                    <  1:3983292/150‑1 (MQ=255)
                                                                                                                    aacaacTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCC‑GAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTA‑GCGGAATTGAt                                   <  1:3468730/150‑1 (MQ=255)
                                                                                                                     acaacTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCC‑GAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTA‑GCGGAATTGAtt                                  <  2:364822/150‑1 (MQ=255)
                                                                                                                      caacTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCC‑GAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTA‑GCGGAATTGATTa                                 <  2:4451384/150‑1 (MQ=255)
                                                                                                                      caacTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCC‑GAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTA‑GCGGAATTGATTa                                 <  2:3923503/150‑1 (MQ=255)
                                                                                                                      caacTCTGTGCAACAGCTGCAAGGGCGGGGGAAGCGAAAAAAACTATTCTTGAGGAGTGAGGCGGGCCTTGACCC‑GAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTA‑GCGGAATTGATTa                                 <  2:1090314/150‑1 (MQ=255)
                                                                                                                                cAACAGCTGCAGGGGCGGGGGAAGCGATAAAATCTTTCCTTGAGGAGTGATGGGGGCCTTGACCC‑GAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTA‑GCGGAATTGATTATCTTTTCGta                       <  2:3190368/150‑1 (MQ=255)
                                                                                                                                 aaCAGCTGCAATGGCGGGGGGAGCGATAAAAAATATTTTTTGGGGGGGATGCGGGGCTTGACCC‑GAAAAGCGGGGAAAGGCCTGCCGATAAGTTGTGATAAGAAAAAAAATTACCCGGTAAGGGTAGGGGGAATTGATT‑TCtttttttat                      >  2:836556/1‑145 (MQ=255)
                                                                                                                                 aaCAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCC‑GAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTA‑GCGGAATTGATTATCTTTTCGtat                      <  1:1028208/150‑1 (MQ=255)
                                                                                                                                    aGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCTTTGACCC‑GAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTA‑GCGGAATTGATTATCTTTTCGTATAAc                   <  2:765175/150‑1 (MQ=255)
                                                                                                                                    aGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCC‑GAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTA‑GCGGAATTGATTATCTTTTCGTATAAc                   <  1:3702924/150‑1 (MQ=255)
                                                                                                                                       tGCAATGGCGGGGGAAGCGATAAAAAATATTTTTTGGGGGGGATGCGGGGCTTGACCC‑CAAAAGGGGGGAAACGCCCGCCGGTAAGTTTTGGAAAAACAAAAAATTACGCGTTTAAGGTA‑GGGGGATTTTTTTTTTTTTTTTATaaaaaa                >  2:3312130/1‑146 (MQ=255)
                                                                                                                                         cagtGGCGGGGGAATCGATAAAATCTATTCTTGAGGAGGGATGCGGGCTTTGACCG‑GAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTA‑GCGGAATTGATTATCTTTTCGTATAACGATAg              <  2:2893434/147‑1 (MQ=255)
                                                                                                                                         cAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCC‑GAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTA‑GCGGAATTGATTATCTTTTCGTATAACGATAg              <  2:4106223/150‑1 (MQ=255)
                                                                                                                                         cAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCC‑GAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTA‑GCGGAATTGATTATCTTTTCGTATAACGATAg              <  2:1697922/150‑1 (MQ=255)
                                                                                                                                         cAATGGCGGGGGAAGCGAAAAAAACTATTCTTGAGGAGTGATGCGGGCCTAGACCC‑GAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTA‑GCGGAATTGATTATCTTTTCGTATAACGATAg              <  2:3821438/150‑1 (MQ=255)
                                                                                                                                            tGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCC‑GAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTA‑GCGGAATTGATTATCTTTTCGTATAACGATAGaaa           <  2:2204885/150‑1 (MQ=255)
                                                                                                                                             ggCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCC‑GAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTA‑GCGGAATTGATTATCTTTTCGTATAAAGATAGAAAt          >  2:2692401/1‑150 (MQ=255)
                                                                                                                                                gggggAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCC‑GAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTA‑GCGGAATTGATTATCTTTTCGTATAACGATAGAAATGaa       <  1:2469820/150‑1 (MQ=255)
                                                                                                                                                gggggAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCC‑GAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTA‑GCGGAATTGATTATCTTTTCGTATAACGATAGAAATGaa       <  1:1635872/150‑1 (MQ=255)
                                                                                                                                                 ggggAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCC‑GAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTA‑GCGGAATTGATTATCTTTTCGTATAACGATAGAAATGaaa      <  1:2184823/150‑1 (MQ=255)
                                                                                                                                                 ggggAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCC‑GAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTA‑GCGGAATTGATTATCTTTTCGTATAACGATAGAAATGaaa      >  1:1769509/1‑150 (MQ=255)
                                                                                                                                                  gggAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCC‑GAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTA‑GCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAAc     <  2:4188311/150‑1 (MQ=255)
                                                                                                                                                    gAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCC‑GAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTA‑GCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAACgt   >  1:1500736/1‑150 (MQ=255)
                                                                                                                                                     aaGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCC‑GAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTA‑GCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAACgtt  <  1:1887756/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                       
GACCATTTATGACTACATCGCCAGGCGGCATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCC‑GAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTA‑GCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAACGTT  >  AP012306/3903642‑3903940

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑