Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP012306202,4170TG24.6% 676.7 / 37.5 302V409G (GTA→GGA) tilStRNA(Ile)‑lysidine synthetase
Reads supporting (aligned to +/- strand):  ref base T (98/129);  new base G (74/0);  total (173/129)
Fisher's exact test for biased strand distribution p-value = 2.40e-22
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 351 total aligned reads displayed.

TTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGC‑AAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGATTCGCTTACCACCACCGTACCG  >  AP012306/202269‑202566
                                                                                                                                                    |                                                                                                                                                      
  cAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGGGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTAtt                                                                                                                                                     <  2:3044079/150‑1 (MQ=255)
      gcgcCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTtgtg                                                                                                                                                 <  1:2610792/150‑1 (MQ=255)
        gcCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCCGGGGGATTTGTGAc                                                                                                                                               >  1:920181/1‑150 (MQ=255)
        gcCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGGATTtttgga                                                                                                                                               >  2:501318/1‑148 (MQ=255)
         ccAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCTCTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGGATTTGTGACg                                                                                                                                              >  1:245355/1‑150 (MQ=255)
           aGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGGATTTGTGACGC‑a                                                                                                                                           >  1:1640289/1‑150 (MQ=255)
            ggATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCCGGGGGATTTTTGGCGC‑aa                                                                                                                                          >  2:1655415/1‑150 (MQ=255)
              aTTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGGATTTGTGACGC‑aaga                                                                                                                                        >  1:2099816/1‑150 (MQ=255)
                  ctgcATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGGAGGGGGCTTTTTGACGC‑cagaaagg                                                                                                                                    >  2:1920154/1‑147 (MQ=255)
                  ctgcATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGGATTTGTGACGC‑AAGAAGgt                                                                                                                                    >  1:2394844/1‑150 (MQ=255)
                     cATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGC‑AAGAAGGtgtg                                                                                                                                 <  1:2158469/150‑1 (MQ=255)
                      atatTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGGGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTTTGGCGAAACGCTGATTGCGGCGGGCGGGGGGTTTGTGGCGC‑AAGAAggggggg                                                                                                                                >  2:2960826/1‑145 (MQ=255)
                       tatTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCCGGGGGATTTTTGGCGC‑CAGAAGGGGTggg                                                                                                                               >  2:3372025/1‑149 (MQ=255)
                       tatTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGGATTTGTGACGC‑CAGAAGGTGTGGc                                                                                                                               >  1:1557225/1‑150 (MQ=255)
                         ttGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGC‑AAGAAGGGGTGGCTg                                                                                                                             >  1:3850546/1‑150 (MQ=255)
                         ttGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGGATTTGTGACGC‑AAGAAGGTGTGGCTg                                                                                                                             >  1:1799349/1‑150 (MQ=255)
                               ggagTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTCCGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGC‑AAGAAGGTGTGGCTGAAGGTg                                                                                                                       <  1:1353451/147‑1 (MQ=255)
                                gCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGC‑AAGAAGGTGTGGCTGGAGGTga                                                                                                                      >  1:1187311/1‑150 (MQ=255)
                                gCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGGATTTGTGACGC‑AAGAAGGTGTGGCTGAAGGTga                                                                                                                      >  1:4683596/1‑150 (MQ=255)
                                 cGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGCTTGCGGCGGCAGGGGGATTTTTGGAGCAAAAAAGGGGGGGCGGAAggggg                                                                                                                      >  2:1071909/1‑147 (MQ=255)
                                 cGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGGATTTGTGACGC‑AAGAAGGTGTGGCTGAAGGTgag                                                                                                                     >  1:3254307/1‑150 (MQ=255)
                                  gTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGGATTTGTGACGC‑AAGAAAGTTTGGGTGAAgggggga                                                                                                                    >  1:1964877/1‑145 (MQ=255)
                                   tAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGGATTTGTGACGC‑AAGAAGGTGTGGCTGAAGGGGGGaa                                                                                                                   >  1:2899385/1‑150 (MQ=255)
                                   tAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGGGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGGCGGGGGATTTTTGACGC‑GAGAAGGGGTGGGTGGAGGGgagga                                                                                                                   >  2:2881648/1‑148 (MQ=255)
                                     aCGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGC‑AAGAAGGTGTGGCTGAAGGTGAGAATg                                                                                                                 >  1:998213/1‑150 (MQ=255)
                                      cggcggACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGC‑AAGAAGGTGTGGCTGAAGGGGAGAATgg                                                                                                                >  1:3465168/1‑150 (MQ=255)
                                      cggcggACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGGATTTTTGACGC‑AAGAAGGTGTGGCTGAAGGGGAGAATgg                                                                                                                >  2:3924548/1‑150 (MQ=255)
                                             cGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGGATTTGTGACGC‑AAGAAGGTGTGGCTGAAGGGGAGAATGGGGGAAGt                                                                                                         >  2:2067445/1‑150 (MQ=255)
                                               tAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGGATTTGTGACGC‑AAGAAGGTGGGGCTGAAGGGGAGAATGGCGTAAGttt                                                                                                       >  2:2774153/1‑150 (MQ=255)
                                                   cTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGC‑AAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTc                                                                                                   >  1:4217676/1‑150 (MQ=255)
                                                    tAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGC‑AAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCt                                                                                                  >  1:3146402/1‑150 (MQ=255)
                                                    tAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGC‑AAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCt                                                                                                  <  1:2995120/150‑1 (MQ=255)
                                                     aaaGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGGATTTGTGACGC‑AAGAAGGTGGGGCTGAAGGGGAGAATGGCCTAAGGtttgtttg                                                                                                 >  2:3101473/1‑150 (MQ=255)
                                                      aaGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGGATTTGTGACGC‑AAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTgg                                                                                                >  1:2744880/1‑150 (MQ=255)
                                                       aGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGGATTTGTGACGC‑AAGAAGGTGTGGCTGAAGGGGAGAATGGCGTAAGTTTTGTCTGGc                                                                                               >  2:3386045/1‑150 (MQ=255)
                                                         aaaaTCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGC‑AAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAg                                                                                             <  2:3428279/150‑1 (MQ=255)
                                                            aTCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGC‑AAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGaaa                                                                                          <  1:4566262/150‑1 (MQ=255)
                                                             tCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGC‑AAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGaaaa                                                                                         <  2:2816174/150‑1 (MQ=255)
                                                               tGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGC‑AAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACg                                                                                       >  1:597111/1‑150 (MQ=255)
                                                                ggCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGC‑AAGAAGGTGTGGCTGAAGGGGAGAATGGCGTAAGTTTTTTCTGGCAGAAAACGc                                                                                      >  1:1160805/1‑150 (MQ=255)
                                                                 gCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGGTTGCGGCGGCATGGGACTTTTTTTCGC‑CAGAAAGCGAGGCTGATGGGGCAGATGGAGTAAGGTTTTGCTTGCAGCAAACGCt                                                                                     >  2:1739919/1‑150 (MQ=255)
                                                                  cAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGC‑AAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCtt                                                                                    <  1:4131053/150‑1 (MQ=255)
                                                                    agagCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGC‑AAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAg                                                                                  >  2:3840275/1‑150 (MQ=255)
                                                                     gagCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGC‑AAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTTTCTTGCAGAAAACGCTTTGt                                                                                 >  1:4321735/1‑150 (MQ=255)
                                                                     gagCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGGATTTGTGACGG‑CAGAAGGGGTGGCTGAAGGTGAGGATGGCGAAAGTTTTGTCTGGCAGAAAACGCTTAgg                                                                                 >  2:4100610/1‑149 (MQ=255)
                                                                     gagCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGGATTTGTGACGC‑AAGAAGGTGTGGCTGAAGGGGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGt                                                                                 >  2:3655608/1‑150 (MQ=255)
                                                                       gCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGC‑AAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTa                                                                               <  1:4458932/150‑1 (MQ=255)
                                                                       gCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGC‑AAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTa                                                                               >  1:3933263/1‑150 (MQ=255)
                                                                            gCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGC‑AAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGa                                                                          >  1:2815176/1‑150 (MQ=255)
                                                                            gCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGC‑AAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGa                                                                          <  2:2142257/150‑1 (MQ=255)
                                                                              gTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGGATTTGGGAAGC‑AAGAAGGGGGGGCTGAAGGTGAGAATGGCGTAAGGTTTGTCTTGCAGAAAACGCTTAGTTAAGTGaaa                                                                        >  2:3267143/1‑150 (MQ=255)
                                                                                gccgccGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGC‑AAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGc                                                                      <  2:3343832/150‑1 (MQ=255)
                                                                                gccgccGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGC‑AAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGc                                                                      <  1:73539/150‑1 (MQ=255)
                                                                                gccgccGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGC‑AAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGc                                                                      >  1:1905868/1‑150 (MQ=255)
                                                                                  cgccgTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGC‑AAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCg                                                                    >  1:4508365/1‑150 (MQ=255)
                                                                                     cgTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGC‑AAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGAt                                                                 <  2:4359070/150‑1 (MQ=255)
                                                                                     cgTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGGATTTGTGACGC‑AAGAAGGTGTGGCTGAAGGGGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGAt                                                                 >  2:4316892/1‑150 (MQ=255)
                                                                                      gTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGC‑AAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATa                                                                <  1:2965517/150‑1 (MQ=255)
                                                                                       tGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGC‑AAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATaa                                                               <  1:1035354/150‑1 (MQ=255)
                                                                                         gCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGC‑AAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGa                                                             <  1:3991628/150‑1 (MQ=255)
                                                                                         gCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGC‑AAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGa                                                             <  1:1737418/150‑1 (MQ=255)
                                                                                         gCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGC‑AAGAAGGTGTGGCTGAAGGGGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAACCGGATAAGa                                                             >  2:1271312/1‑150 (MQ=255)
                                                                                         gCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGC‑AAGAAGGTGTGGCTGAAGGGGAGAATGGCGTAAGGTTTGTCTGGCAGAAAAAGCTTAGTTAAGGGAAAACCGGATAAGa                                                             >  2:4634780/1‑150 (MQ=255)
                                                                                                gACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGC‑AAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCa                                                      <  2:3454015/150‑1 (MQ=255)
                                                                                                 acacCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGGATTTGTGACGC‑AAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGGTAAGACGCCTCaa                                                     >  2:3972724/1‑150 (MQ=255)
                                                                                                  caccacGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCCGGGGGATTTGTGACGC‑AAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCaaa                                                    >  2:2509377/1‑150 (MQ=255)
                                                                                                      acGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGC‑AAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTc                                                >  1:3966829/1‑150 (MQ=255)
                                                                                                           aCTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGC‑AAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATc                                           <  2:2253415/150‑1 (MQ=255)
                                                                                                            ctgctgTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGC‑AAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATcc                                          <  2:4391944/150‑1 (MQ=255)
                                                                                                              gctgTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGC‑AAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCgg                                        >  1:4614874/1‑150 (MQ=255)
                                                                                                              gctgTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGC‑AAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCgg                                        >  1:1397802/1‑150 (MQ=255)
                                                                                                               ctgTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGC‑AAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGc                                       <  2:3176759/150‑1 (MQ=255)
                                                                                                               ctgTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGC‑AAGAAGGTGTGGCTGAAGGGGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGc                                       >  2:694618/1‑150 (MQ=255)
                                                                                                                 gTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGC‑AAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGa                                     >  1:3628451/1‑150 (MQ=255)
                                                                                                                   tttATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGC‑AAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGaaa                                   >  1:3662282/1‑150 (MQ=255)
                                                                                                                      aTGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGC‑AAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTc                                <  2:4056965/150‑1 (MQ=255)
                                                                                                                      aTGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGC‑AAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTc                                >  1:798144/1‑150 (MQ=255)
                                                                                                                       tGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGC‑AAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCa                               <  1:517095/150‑1 (MQ=255)
                                                                                                                          cGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGC‑AAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATc                            >  1:541867/1‑150 (MQ=255)
                                                                                                                           gAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGC‑AAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCa                           <  2:1483022/150‑1 (MQ=255)
                                                                                                                           gAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGC‑AAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCa                           <  2:4471920/150‑1 (MQ=255)
                                                                                                                           gAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGC‑AAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCa                           <  2:1589548/150‑1 (MQ=255)
                                                                                                                            aaaCGCTGATTGCGGCGGCAGGGGTATTTGTGACGC‑AAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAg                          <  1:685808/150‑1 (MQ=255)
                                                                                                                             aaCGCTGATTGCGGCGGCAGGGGTATTTGTGACGC‑AAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAgg                         <  2:254955/150‑1 (MQ=255)
                                                                                                                              aCGCTGATTGCGGCGGCAGGGGTATTTGTGACGC‑AAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGa                        <  2:4530434/150‑1 (MQ=255)
                                                                                                                               cGCTGATTGCGGCGGCAGGGGTATTTGTGACGC‑AAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGAt                       >  1:4013656/1‑150 (MQ=255)
                                                                                                                               cGCTGATTGCGGCGGCAGGGGTATTTGTGACGC‑AAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGAt                       <  1:1279370/150‑1 (MQ=255)
                                                                                                                               cGCTGATTGCGGCGGCAGGGGTATTTGTGACGC‑AAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGAt                       <  2:3211884/150‑1 (MQ=255)
                                                                                                                                gCTGATTGCGGCGGCAGGGGTATTTGTGACGC‑AAGAAGGTGTGGCTGAAGGGGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGAtt                      >  2:2381425/1‑150 (MQ=255)
                                                                                                                                 cTGATTGCGGCGGCAGGGGGATTTGTGACGC‑AAGAAGGTGTGGCTGAAGGTGAGAATGGCGGAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAACCCGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGATTc                     >  2:1423509/1‑150 (MQ=255)
                                                                                                                                  tGATTGCGGCGGCAGGGGTATTTGTGACGC‑AAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGATTCg                    <  1:4545353/150‑1 (MQ=255)
                                                                                                                                  tGATTGCGGCGGCAGGGGTATTTGTGACGC‑AAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGATTCg                    <  2:810210/150‑1 (MQ=255)
                                                                                                                                  tGATTGCGGCGGCAGGGGTATTTGTGACGC‑AAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGATTCg                    >  2:4194843/1‑150 (MQ=255)
                                                                                                                                  tGATTGCGGCGGCAGGGGTATTTGTGACGC‑AAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGATTCg                    >  1:1695464/1‑150 (MQ=255)
                                                                                                                                   gATTGCGGCGGCAGGGGTATTTGTGACGC‑AAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGATTCGc                   <  1:3070309/150‑1 (MQ=255)
                                                                                                                                      tGCGGCGGCAGGGGTATTTGTGACGC‑AAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGATTCGCTTa                <  1:3588920/150‑1 (MQ=255)
                                                                                                                                         ggcggcAGGGGTATTTGTGACGC‑AAGAAGGTGTGGCTGAAGGTGAGAAGGGCGTAAGTGTTGTCTGGCCGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAGCGGCGCATCCGGCGAAAGTCAATCAGGATTCGCTTacca             <  2:1250497/150‑1 (MQ=255)
                                                                                                                                                ggggTATTTGTGACGC‑AAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGATTCGCTTACCACCACCGt      <  2:1536706/150‑1 (MQ=255)
                                                                                                                                                 gggTATTTGTGACGC‑AAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGATTCGCTTACCACCACCGTa     >  2:2668142/1‑150 (MQ=255)
                                                                                                                                                    tATTTGTGACGC‑AAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGATTCGCTTACCACCACCGTACCg  >  2:558157/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                      
TTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGC‑AAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGATTCGCTTACCACCACCGTACCG  >  AP012306/202269‑202566

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑