Predicted mutation
evidence seq id position mutation annotation gene description
RA AP012306 3,252,721 G→A G370D (GGT→GAT)  spoT → bifunctional (p)ppGpp synthetase II/guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123063,252,7210GA100.0% 1119.7 / NA 287G370D (GGT→GAT) spoTbifunctional (p)ppGpp synthetase II/guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (107/180);  total (107/180)

Only 100 of 292 total aligned reads displayed.

AGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGCCGCGCACTGGGCTTATAAAGAGCACGGCGAAACCAGTACTACCGCACAAATCCGCGCCCAGCGCTGGATGCAAAGCCTGCTGGAGCTGCAACAGAGCGCCGGTAGTTCGTTTGAATTTATCGAGAGCGTTAAATCCGATCTCTTCCCGGATGAGATTTACGTTTTCACACCGGAAGGGCGCATTGTCGAGCTGCCTGCCGGTGCAACGCCCGTCGACTTCGCTTATGCAGTGCATACCGATATCGGTC  >  AP012306/3252574‑3252867
                                                                                                                                                   |                                                                                                                                                  
aGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGCCGCGCACTGGGCTTATAAAGAGCACGGCGAAACCAGTACTACCGCACAAATCCGCGCCCAGCGCTGGATGCAAAGCCTGCAGGAGCTGCAACAGAGCGCCGATa                                                                                                                                                  <  1:2776797/150‑1 (MQ=255)
 gATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGCCGCGCACTGGGCTTATAAAGAGCACGGCGAAACCAGTACTACCGCACAAATCCGCGCCCAGCGCTGGATGCAAAGCCTGCTGGAGCTGCAACAGAGCGCCGATAg                                                                                                                                                 <  1:87667/150‑1 (MQ=255)
 gATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGCCGCGCACTGGGCTTATAAAGAGCACGGCGAAACCAGTACTACCGCACAAATCCGCGCCCAGCGCTGGATGCAAAGCCTGCTGGAGCTGCAACAGAGCGCCGATAg                                                                                                                                                 <  1:1942816/150‑1 (MQ=255)
  aTCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGCCGCGCACTGGGCTTATAAAGAGCACGGCGAAACCAGTACTACCGCACAAATCCGCGCCCAGCGCTGGATGCAAAGCCTGCTGGAGCTGCAACAGAGCGCCGATAGt                                                                                                                                                >  1:4487903/1‑150 (MQ=255)
   tCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGCCGCGCACTGGGCTTATAAAGAGCACGGCGAAACCAGTACTACCGCACAAATCCGCGCCCAGCGCTGGATGCAAAGCCTGCTGGAGCTGCAACAGAGCGCCGATAGtt                                                                                                                                               <  2:3692154/150‑1 (MQ=255)
   tCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGCCGCGCACTGGGCTTATAAAGAGCACGGCGAAACCAGTACTACCGCACAAATCCGCGCCCAGCGCTGGATGCAAAGCCTGCTGGAGCTGCAACAGAGCGCCGATAGtt                                                                                                                                               <  2:4247076/150‑1 (MQ=255)
   tCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGCCGCGCACTGGGCTTATAAAGAGCACGGCGAAACCAGTACTACCGCACAAATCCGCGCCCAGCGCTGGATGCAAAGCCTGCTGGAGCTGCAACAGAGCGCCGATAGtt                                                                                                                                               >  1:2191207/1‑150 (MQ=255)
    ccGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGCCGCGCACTGGGCTTATAAAGAGCACGGCGAAACCAGTACTACCGCACAAATCCGCGCCCAGCGCTGGATGCAAAGCCTGCTGGAGCTGCAACAGAGCGCCGATAGTTc                                                                                                                                              <  2:4185492/150‑1 (MQ=255)
    ccGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGCCGCGCACTGGGCTTATAAAGAGCACGGCGAAACCAGTACTACCGCACAAATCCGCGCCCAGCGCTGGATGCAAAGCCTGCTGGAGCTGCAACAGAGCGCCGATAGTTc                                                                                                                                              <  2:1492288/150‑1 (MQ=255)
    ccGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGCCGCGCACTGGGCTTATAAAGAGCACGGCGAAACCAGTACTACCGCACAAATCCGCGCCCAGCGCTGGATGCAAAGCCTGCTGGAGCTGCAACAGAGCGCCGATAGTTc                                                                                                                                              <  1:1368225/150‑1 (MQ=255)
     cGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGCCGCGCACTGGGCTTATAAAGAGCACGGCGAAACCAGTACTACCGCACAAATCCGCGCCCAGCGCTGGATGCAAAGCCTGCTGGAGCTGCAACAGAGCGCCGATAGTTCg                                                                                                                                             <  2:1260074/150‑1 (MQ=255)
      gTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGCCGCGCACTGGGCTTATAAAGAGCACGGCGAAACCAGTACTACCGCACAAATCCGCGCCCAGCGCTGGATGCAAAGCCTGCTGGAGCTGCAACAGAGCGCCGATAGTTCGt                                                                                                                                            <  2:2959352/150‑1 (MQ=255)
         ccGAAGATATGGACCAGATGGCGGAGATGGGTGTTGCCGCGCACTGGGCTTATAAAGAGCACGGCGAAACCAGTACTACCGCACAAATCCGCGCCCAGCGCTGGATGCAAAGCCTGCTGGAGCTGCAACAGAGCGCCGATAGTTCGTTTg                                                                                                                                         <  1:781008/150‑1 (MQ=255)
          cGAAGATATGGACCAGATGGCGGAGATGGGTGTTGCCGCGCACTGGGCTTATAAAGAGCACGGCGAAACCAGTACTACCGCACAAATCCGCGCCCAGCGCTGGATGCAAAGCCTGCTGGAGCTGCAACAGAGCGCCGATAGTTCGTTTGa                                                                                                                                        <  1:4113559/150‑1 (MQ=255)
            aaGATATGGACCAGATGGCGGAGATGGGTGTTGCCGCGCACTGGGCTTATAAAGAGCACGGCGAAACCAGTACTACCGCACAAATCCGCGCCCAGCGCTGGATGCAAAGCCTGCTGGAGCTGCAACAGAGCGCCGATAGTTCGTTTGAAt                                                                                                                                      <  1:616334/150‑1 (MQ=255)
             aGATATGGACCAGATGGCGGAGATGGGTGTTGCCGCGCACTGGGCTTATAAAGAGCACGGCGAAACCAGTACTACCGCACAAATCCGCGCCCAGCGCTGGATGCAAAGCCTGCTGGAGCTGCAACAGAGCGCCGATAGTTCGTTTGAAtt                                                                                                                                     <  1:339096/150‑1 (MQ=255)
             aGATATGGACCAGATGGCGGAGATGGGTGTTGCCGCGCACTGGGCTTATAAAGAGCACGGCGAAACCAGTACTACCGCACAAATCCGCGCCCAGCGCTGGATGCAAAGCCTGCTGGAGCTGCAACAGAGCGCCGATAGTTCGTTTGAAtt                                                                                                                                     >  1:3681417/1‑150 (MQ=255)
              gATATGGACCAGATGGCGGAGATGGGTGTTGCCGCGCACTGGGCTTATAAAGAGCACGGCGAAACCAGTACTACCGCACAAATCCGCGCCCAGCGCTGGATGCAAAGCCTGCTGGAGCTGCAACAGAGCGCCGATAGTTCGTTTGAAttt                                                                                                                                    <  2:2879599/150‑1 (MQ=255)
               atatGGACCAGATGGCGGAGATGGGTGTTGCCGCGCACTGGGCTTATAAAGAGCACGGCGAAACCAGTACTACCGCACAAATCCGCGCCCAGCGCTGGATGCAAAGCCTGCTGGAGCTGCAACAGAGCGCCGATAGTTCGTTTGAATTTa                                                                                                                                   >  2:511745/1‑150 (MQ=255)
               atatGGACCAGATGGCGGAGATGGGTGTTGCCGCGCACTGGGCTTATAAAGAGCACGGCGAAACCAGTACTACCGCACAAATCCGCGCCCAGCGCTGGATGCAAAGCCTGCTGGAGCTGCAACAGAGCGCCGATAGTTCGTTTGAATTTa                                                                                                                                   <  1:933152/150‑1 (MQ=255)
                tatGGACCAGATGGCGGAGATGGGTGTTGCCGCGCACTGGGCTTATAAAGAGCACGGCGAAACCAGTACTACCGCACAAATCCGCGCCCAGCGCTGGATGCAAAGCCTGCTGGAGCTGCAACAGAGCGCCGATAGTTCGTTTGAATTTAt                                                                                                                                  <  2:2058208/150‑1 (MQ=255)
                 atGGACCAGATGGCGGAGATGGGTGTTGCCGCGCACTGGGCTTATAAAGAGCACGGCGAAACCAGTACTACCGCACAAATCCGCGCCCAGCGCTGGATGCAAAGCCTGCTGGAGCTGCAACAGAGCGCCGATAGTTCGTTTGAATTTATc                                                                                                                                 <  2:3899467/150‑1 (MQ=255)
                  tGGACCAGATGGCGGAGATGGGTGTTGCCGCGCACTGGGCTTATAAAGAGCACGGCGAAACCAGTACTACCGCACAAATCCGCGCCCAGCGCTGGATGCAAAGCCTGCTGGAGCTGCAACAGAGCGCCGATAGTTCGTTTGAATTTATCg                                                                                                                                <  1:841066/150‑1 (MQ=255)
                  tGGACCAGATGGCGGAGATGGGTGTTGCCGCGCACTGGGCTTATAAAGAGCACGGCGAAACCAGTACTACCGCACAAATCCGCGCCCAGCGCTGGATGCAAAGCCTGCTGGAGCTGCAACAGAGCGCCGATAGTTCGTTTGAATTTATCg                                                                                                                                <  2:4395707/150‑1 (MQ=255)
                    gACCAGATGGCGGAGATGGGTGTTGCCGCGCACTGGGCTTATAAAGAGCACGGCGAAACCAGTACTACCGCACAAATCCGCGCCCAGCGCTGGATGCAAAGCCTGCTGGAGCTGCAACAGAGCGCCGATAGTTCGTTTGAATTTATCgag                                                                                                                              >  1:1139776/1‑150 (MQ=255)
                    gACCAGATGGCGGAGATGGGTGTTGCCGCGCACTGGGCTTATAAAGAGCACGGCGAAAACAGTACTACCGCACAAATCCGCGCCCAGCGCTCGATGCGAAGCCTGCTGGAGCTGCAACAGAGCGCCGATAGTTCGTTTGAATTTATCgag                                                                                                                              >  2:1360973/1‑150 (MQ=255)
                     aCCAGATGGCGGAGATGGGTGTTGCCGCGCACTGGGCTTATAAAGAGCACGGCGAAACCAGTACTACCGCACAAATCCGCGCCCAGCGCTGGATGCAAAGCCTGCTGGAGCTGCAACAGAGCGCCGATAGTTCGTTTGAATTTATCgaga                                                                                                                             >  2:2435721/1‑150 (MQ=255)
                       cAGATGGCGGAGATGGGTGTTGCCGCGCACTGGGCTTATAAAGAGCACGGCGAAACCAGTACTACCGCACAAATCCGCGCCCAGCGCTGGATGCAAAGCCTGCTGGAGCTGCAACAGAGCGCCGATAGTTCGTTTGAATTTATCGAGAGc                                                                                                                           >  1:4046758/1‑150 (MQ=255)
                           tGGCGGAGATGGGTGTTGCCGCGCACTGGGCTTATAAAGAGCACGGCGAAACCAGTACTACCGCACAAATCCGCGCCCAGCGCTGGATGCAAAGCCTGCTGGAGCTGCAACAGAGCGCCGATAGTTCGTTTGAATTTATCGAGAGCGTTa                                                                                                                       <  2:4716260/150‑1 (MQ=255)
                             gCGGAGATGGGTGTTGCCGCGCACTGGGCTTATAAAGAGCACGGCGAAACCAGTACTACCGCACAAATCCGCGCCCAGCGCTGGATGCAAAGCCTGCTGGAGCTGCAACAGAGCGCCGATAGTTCGTTTGAATTTATCGAGAGCGTTaaa                                                                                                                     <  2:1697954/150‑1 (MQ=255)
                               ggAGATGGGTGTTGCCGCGCACTGGGCTTATAAAGAGCACGGCGAAACCAGTACTACCGCACAAATCCGCGCCCAGCGCTGGATGCAAAGCCTGCTGGAGCTGCAACAGAGCGCCGATAGTTCGTTTGAATTTATCGAGAGCGTTAAATc                                                                                                                   <  1:1027165/150‑1 (MQ=255)
                               ggAGATGGGTGTTGCCGCGCACTGGGCTTATAAAGAGCACGGCGAAACCAGTACTACCGCACAAATCCGCGCCCAGCGCTGGATGCAAAGCCTGCTGGAGCTGCAACAGAGCGCCGATAGTTCGTTTGAATTTATCGAGAGCGTTAAATc                                                                                                                   >  2:3621699/1‑150 (MQ=255)
                               ggAGATGGGTGTTGCCGCGCACTGGGCTTATAAAGAGCACGGCGAAACCAGTACTACCGCACAAATCCGCGCCCAGCGCTGGATGCAAAGCCTGCTGGAGCTGCAACAGAGCGCCGATAGTTCGTTTGAATTTATCGAGAGCGTTAAATc                                                                                                                   >  2:3470435/1‑150 (MQ=255)
                                gagaTGGGTGTTGCCGCGCACTGGGCTTATAAAGAGCACGGCGAAACCAGTACTACCGCACAAATCCGCGCCCAGCGCTGGATGCAAAGCCTGCTGGAGCTGCAACAGAGCGCCGATAGTTCGTTTGAATTTATCGAGAGCGTTAAATcc                                                                                                                  <  2:68440/150‑1 (MQ=255)
                                   aTGGGTGTTGCCGCGCACTGGGCTTATAAAGAGCACGGCGAAACCAGTACTACCGCACAAATCCGCGCCCAGCGCTGGATGCAAAGCCTGCTGGAGCTGCAACAGAGCGCCGATAGTTCGTTTGAATTTATCGAGAGCGTTAAATCCGAt                                                                                                               >  2:3335451/1‑150 (MQ=255)
                                    tGGGTGTTGCCGCGCACTGGGCTTATAAAGAGCACGGCGAAACCAGTACTACCGCACAAATCCGCGCCCAGCGCTGGATGCAAAGCCTGCTGGAGCTGCAACAGAGCGCCGATAGTTCGTTTGAATTTATCGAGAGCGTTAAATCCGAtc                                                                                                              <  2:2799829/150‑1 (MQ=255)
                                     gggTGTTGCCGCGCACTGGGCTTATAAAGAGCACTGCGAAACCAGTACTACCGCACAAATCCGCGCCCAGCGCTGGATGCAAAGCCTGCTGGAGCTGCAACAGAGCGCCGATAGTTCGTTTGAATTTATCGAGAGCGTTAAATCCGAtct                                                                                                             >  1:3045159/1‑150 (MQ=255)
                                       gtgtTGCCGCGCACTGGGCTTATAAAGAGCACGGCGAAACCAGTACTACCGCACAAATCCGCGCCCAGCGCTGGATGCAAAGCCTGCTGGAGCTGCAACAGAGCGCCGATAGTTCGTTTGAATTTATCGAGAGCGTTAAATCCGATctct                                                                                                           <  1:17949/150‑1 (MQ=255)
                                            gCCGCGCACTGGGCTTATAAAGAGCACGGCGAAACCAGTACTACCGCACAAATCCGCGCCCAGCGCTGGATGCAAAGCCTGCTGGAGCTGCAACAGAGCGCCGATAGTTCGTTTGAATTTATCGAGAGCGTTAAATCCGATCTCTTCCCg                                                                                                      <  2:4141418/150‑1 (MQ=255)
                                               gcgcACTGGGCTTATAAAGAGCACGGCGAAACCAGTACTACCGCACAAATCCGCGCCCAGCGCTGGATGCAAAGCCTGCTGGAGCTGCAACAGAGCGCCGATAGTTCGTTTGAATTTATCGAGAGCGTTAAATCCGATCTCTTCCCGGAt                                                                                                   <  1:2245372/150‑1 (MQ=255)
                                                 gcACTGGGCTTATAAAGAGCACGGCGAAACCAGTACTACCGCACAAATCCGCGCCCAGCGCTGGATGCAAAGCCTGCTGGAGCTGCAACAGAGCGCCGATAGTTCGTTTGAATTTATCGAGAGCGTTAAATCCGATCTCTTCCCGGATga                                                                                                 <  1:3211284/150‑1 (MQ=255)
                                                    cTGGGCTTATAAAGAGCACGGCGAAACCAGTACTACCGCACAAATCCGCGCCCAGCGCTGGATGCAAAGCCTGCTGGAGCTGCAACAGAGCGCCGATAGTTCGTTTGAATTTATCGAGAGCGTTAAATCCGATCTCTTCCCGGATGAGAt                                                                                              <  2:3253295/150‑1 (MQ=255)
                                                     tGGGCTTATAAAGAGCACGGCGAAACCAGTACTACCGCACAAATCCGCGCCCAGCGCTGGATGCAAAGCCTGCTGGAGCTGCAACAGAGCGCCGATAGTTCGTTTGAATTTATCGAGAGCGTTAAATCCGATCTCTTCCCGGATGAGAtt                                                                                             <  2:2714982/150‑1 (MQ=255)
                                                     tGGGCTTATAAAGAGCACGGCGAAACCAGTACTACCGCACAAATCCGCGCCCAGCGCTGGATGCAAAGCCTGCTGGAGCTGCAACAGAGCGCCGATAGTTCGTTTGAATTTATCGAGAGCGTTAAATCCGATCTCTTCCCGGATGAGAtt                                                                                             >  1:1676841/1‑150 (MQ=255)
                                                       ggCTTATAAAGAGCACGGCGAAACCAGTACTACCGCACAAATCCGCGCCCAGCGCTGGATGCAAAGCCTGCTGGAGCTGCAACAGAGCGCCGATAGTTCGTTTGAATTTATCGAGAGCGTTAAATCCGATCTCTTCCCGGATGAGATTTa                                                                                           <  2:3024650/150‑1 (MQ=255)
                                                         cTTATAAAGAGCACGGCGAAACCAGTACTACCGCACAAATCCGCGCCCAGCGCTGGATGCAAAGCCTGCTGGAGCTGCAACAGAGCGCCGATAGATCGTTTGAATTTATCGAGAGCGTTAAATCCGATCTCTTCCCGGATGAGATTTACg                                                                                         >  1:4435693/1‑150 (MQ=255)
                                                             taAAGAGCACGGCGAAACCAGTACTACCGCACAAATCCGCGCCCAGCGCTGGATGCAAAGCCTGCTGGAGCTGCAACAGAGCGCCGATAGTTCGTTTGAATTTATCGAGAGCGTTAAATCCGATCTCTTCCCGGATGAGATTTACGtttt                                                                                     <  1:4238758/150‑1 (MQ=255)
                                                              aaaGAGCACGGCGAAACCAGTACTACCGCACAAATCCGCGCCCAGCGCTGGATGCAAAGCCTGCTGGAGCTGCAACAGAGCGCCGATAGTTCGTTTGAATTTATCGAGAGCGTTAAATCCGATCTCTTCCCGGATGAGATTTACGTTTTc                                                                                    >  2:3852507/1‑150 (MQ=255)
                                                               aaGAGCACGGCGAAACCAGTACTACCGCACAAATCCGCGCCCAGCGCTGGATGCAAAGCCTGCTGGAGCTGCAACAGAGCGCCGATAGTTCGTTTGAATTTATCGAGAGCGTTAAATCCGATCTCTTCCCGGATGAGATTTACGTTTTca                                                                                   >  2:4037176/1‑150 (MQ=255)
                                                                 gagCACGGCGAAACCAGTACTACCGCACAAATCCGCGCCCAGCGCTGGATGCAAAGCCTGCTGGAGCTGCAACAGAGCGCCGATAGTTCGTTTGAATTTATCGAGAGCGTTAAATCCGATCTCTTCCCGGATGAGATTTACGTTTTcaca                                                                                 <  1:4363656/150‑1 (MQ=255)
                                                                 gagCACGGCGAAACCAGTACTACCGCACAAATCCGCGCCCAGCGCTGGATGCAAAGCCTGCTGGAGCTGCAACAGAGCGCCGATAGTTCGTTTGAATTTATCGAGAGCGTTAAATCCGATCTCTTCCCGGATGAGATTTACGTTTTcaca                                                                                 <  1:1827712/150‑1 (MQ=255)
                                                                   gCACGGCGAAACCAGTACTACCGCACAAATCCGCGCCCAGCGCTGGATGCAAAGCCTGCTGGAGCTGCAACAGAGCGCCGATAGTTCGTTTGAATTTATCGAGAGCGTTAAATCCGATCTCTTCCCGGATGAGATTTACGTTTTCACAcc                                                                               <  2:1761986/150‑1 (MQ=255)
                                                                   gCACGGCGAAACCAGTACTACCGCACAAATCCGCGCCCAGCGCTGGATGCAAAGCCTGCTGGAGCTGCAACAGAGCGCCGATAGTTCGTTTGAATTTATCGAGAGCGTTAAATCCGATCTCTTCCCGGATGAGATTTACGTTTTCACAcc                                                                               <  2:246381/150‑1 (MQ=255)
                                                                   gCACGGCGAAACCAGTACTACCGCACAAATCCGCGCCCAGCGCTGGATGCAAAGCCTGCTGGAGCTGCAACAGAGCGCCGATAGTTCGTTTGAATTTATCGAGAGCGTTAAATCCGATCTCTTCCCGGATGAGATTTACGTTTTCACAcc                                                                               <  1:3528659/150‑1 (MQ=255)
                                                                     aCGGCGAAACCAGTACTACCGCACAAATCCGCGCCCAGCGCTGGATGCAAAGCCTGCTGGAGCTGCAACAGAGCGCCGATAGTTCGTTTGAATTTATCGAGAGCGTTAAATCCGATCTCTTCCCGGATGAGATTTACGTTTTCACACCgg                                                                             >  1:3268757/1‑150 (MQ=255)
                                                                     aCGGCGAAACCAGTACTACCGCACAAATCCGCGCCCAGCGCTGGATGCAAAGCCTGCTGGAGCTGCAACAGAGCGCCGATAGTTCGTTTGAATTTATCGAGAGCGTTAAATCCGATCTCTTCCCGGATGAGATTTACGTTTTCACACCgg                                                                             <  1:1546787/150‑1 (MQ=255)
                                                                      cGGCGAAACCAGTACTACCGCACAAATCCGCGCCCAGCGCTGGATGCAAAGCCTGCTGGAGCTGCAACAGAGCGCCGATAGTTCGTTTGAATTTATCGAGAGCGTTAAATCCGATCTCTTCCCGGATGAGATTTACGTTTTCACACCGGa                                                                            <  1:458846/150‑1 (MQ=255)
                                                                         cGAAACCAGTACTACCGCACAAATCCGCGCCCAGCGCTGGATGCAAAGCCTGCTGGAGCTGCAACAGAGCGCCGATAGTTCGTTTGAATTTATCGAGAGCGTTAAATCCGATCTCTTCCCGGATGAGATTTACGTTTTCACACCGGAAgg                                                                         <  1:3433005/150‑1 (MQ=255)
                                                                         cGAAACCAGTACTACCGCACAAATCCGCGCCCAGCGCTGGATGCAAAGCCTGCTGGAGCTGCAACAGAGCGCCGATAGTTCGTTTGAATTTATCGAGAGCGTTAAATCCGATCTCTTCCCGGATGAGATTTACGTTTTCACACCGGAAgg                                                                         <  2:3291016/150‑1 (MQ=255)
                                                                           aaaCCAGTACTACCGCACAAATCCGCGCCCAGCGCTGGATGCAAAGCCTGCTGGAGCTGCAACAGAGCGCCGATAGTTCGTTTGAATTTATCGAGAGCGTTAAATCCGATCTCTTCCCGGATGAGATTTACGTTTTCACACCGGAAGGgc                                                                       <  1:3706713/150‑1 (MQ=255)
                                                                                aGTACTACCGCACAAATCCGCGCCCAGCGCTGGATGCAAAGCCTGCTGGAGCTGCAACAGAGCGCCGATAGTTCGCTTGAATTTATCGAGAGCGTTAAATCCGATCTCTTCCCGGATGAGATTTACGTTTTCACACCGGAAGGGCGCAtt                                                                  >  2:1581149/1‑150 (MQ=255)
                                                                                 gTACTACCGCACAAATCCGCGCCCAGCGCTGGATGCAAAGCCTGCTGGAGCTGCAACAGAGCGCCGATAGTTCGTTTGAATTTATCGAGAGCGTTAAATCCGATCTCTTCCCGGATGAGATTTACGTTTTCACACCGGAAGGGCGCATTg                                                                 <  1:2927202/150‑1 (MQ=255)
                                                                                  tactacCGCACAAATCCGCGCCCAGCGCTGGATGCAAAGCCTGCTGGAGCTGCAACAGAGCGCCGATAGTTCGTTTGAATTTATCGAGAGCGTTAAATCCGATCTCTTCCCGGATGAGATTTACGTTTTCACACCGGAAGGGCGCATTGt                                                                <  2:1150185/150‑1 (MQ=255)
                                                                                     tacCGCACAAATCCGCGCCCAGCGCTGGATGCAAAGCCTGCTGGAGCTGCAACAGAGCGCCGATAGTTCGTTTGAATTTATCGAGAGCGTTAAATCCGATCTCTTCCCGGATGAGATTTACGTTTTCACACCGGAAGGGCGCATTGTCGa                                                             <  2:2252787/150‑1 (MQ=255)
                                                                                       ccGCACAAATCCGCGCCCAGCGCTGGATGCAAAGCCTGCTGGAGCTGCAACAGAGCGCCGATAGTTCGTTTGAATTTATCGAGAGCGTTAAATCCGATCTCTTCCCGGATGAGATTTACGTTTTCACACCGGAAGGGCGCATTGTCGAGc                                                           >  2:3187160/1‑150 (MQ=255)
                                                                                        cGCACAAATCCGCGCCCAGCGCTGGATGCAAAGCCTGCTGGAGCTGCAACAGAGCGCCGATAGTTCGTTTGAATTTATCGAGAGCGTTAAATCCGATCTCTTCCCGGATGAGATTTACGTTTTCACACCGGAAGGGCGCATTGTCGAGct                                                          <  2:2485853/150‑1 (MQ=255)
                                                                                           acaAATCCGCGCCCAGCGCTGGATGCAAAGCCTGCTGGAGCTGCAACAGAGCGCCGATAGTTCGTTTGAATTTATCGAGAGCGTTAAATCCGATCTCTTCCCGGATGAGATTTACGTTTTCACACCGGAAGGGCGCATTGTCGAGctgcc                                                       <  2:2386697/150‑1 (MQ=255)
                                                                                            caAATCCGCGCCCAGCGCTGGATGCAAAGCCTGCTGGAGCTGCAACAGAGCGCCGATAGTTCGTTTGAATTTATCGAGAGCGTTAAATCCGATCTCTTCCCGGATGAGATTTACGTTTTCACACCGGAAGGGCGCATTGTCGAGctgcct                                                      <  1:1639222/150‑1 (MQ=255)
                                                                                                      cccAGCGCTGGATGCAAAGCCTGCTGGAGCTGCAACAGAGCGCCGATAGTTCGTTTGAATTTATCGAGAGCGTTAAATCCGATCTCTTCCCGGATGAGATTTACGTTTTCACACCGGAAGGGCGCATTGTCGAGCTGCCTGCCGGTGCaa                                            >  1:699551/1‑150 (MQ=255)
                                                                                                        cAGCGCTGGATGCAAAGCCTGCTGGAGCTGCAACAGAGCGCCGATAGTTCGTTTGAATTTATCGAGAGCGTTAAATCCGATCTCTTCCCGGATGAGATTTACGTTTTCACACCGGAAGGGCGCATTGTCGAGCTGCCTGCCGGTGCAACg                                          >  2:4640547/1‑150 (MQ=255)
                                                                                                         aGCGCTGGATGCAAAGCCTGCTGGAGCTGCAACAGAGCGCCGATAGTTCGTTTGAATTTATCGAGAGCGTTAAATCCGATCTCTTCCCGGATGAGATTTACGTTTTCACACCGGAAGGGCGCATTGTCGAGCTGCCTGCCGGTGCAACGc                                         >  2:1543227/1‑150 (MQ=255)
                                                                                                         aGCGCTGGATGCAAAGCCTGCTGGAGCTGCAACAGAGCGCCGATAGTTCGTTTGAATTTATCGAGAGCGTTAAATCCGATCTCTTCCCGGATGAGATTTACGTTTTCACACCGGAAGGGCGCATTGTCGAGCTGCCTGCCGGTGCAACGc                                         <  1:1469075/150‑1 (MQ=255)
                                                                                                          gcgcTGGATGCAAAGCCTGCTGGAGCTGCAACAGAGCGCCGATAGTTCGTTTGAATTTATCGAGAGCGTTAAATCCGATCTCTTCCCGGATGAGATTTACGTTTTCACACCGGAAGGGCGCATTGTCGAGCTGCCTGCCGGTGCAACGcc                                        <  1:2303539/150‑1 (MQ=255)
                                                                                                              tGGATGCAAAGCCTGCTGGAGCTGCAACAGAGCGCCGATAGTTCGTTTGAATTTATCGAGAGCGTTAAATCCGATCTCTTCCCGGATGAGATTTACGTTTTCACACCGGAAGGGCGCATTGTCGAGCTGCCTGCCGGTGCAACGCCCGTc                                    <  1:2541455/150‑1 (MQ=255)
                                                                                                              tGGATGCAAAGCCTGCTGGAGCTGCAACAGAGCGCCGATAGTTCGTTTGAATTTATCGAGAGCGTTAAATCCGATCTCTTCCCGGATGAGATTTACGTTTTCACACCGGAAGGGCGCATTGTCGAGCTGCCTGCCGGTGCAACGCCCGTc                                    >  1:468037/1‑150 (MQ=255)
                                                                                                               ggATGCAAAGCCTGCTGGAGCTGCAACAGAGCGCCGATAGTTCGTTTGAATTTATCGAGAGCGTTAAATCCGATCTCTTCCCGGATGAGATTTACGTTTTCACACCGGAAGGGCGCATTGTCGAGCTGCCTGCCGGTGCAACGCCCGTCg                                   <  2:4208465/150‑1 (MQ=255)
                                                                                                               ggATGCAAAGCCTGCTGGAGCTGCAACAGAGCGCCGATAGTTCGTTTGAATTTATCGAGAGCGTTAAATCCGATCTCTTCCCGGATGAGATTTACGTTTTCACACCGGAAGGGCGCATTGTCGAGCTGCCTGCCGGTGCAACGCCCGTCg                                   >  2:768132/1‑150 (MQ=255)
                                                                                                                gATGCAAAGCCTGCTGGAGCTGCAACAGAGCGCCGATAGTTCGTTTGAATTTATCGAGAGCGTTAAATCCGATCTCTTCCCGGATGAGATTTACGTTTTCACACCGGAAGGGCGCATTGTCGAGCTGCCTGCCGGTGCAACGCCCGTCGa                                  >  2:1898556/1‑150 (MQ=255)
                                                                                                                  tGCAAAGCCTGCTGGAGCTGCAACAGAGCGCCGATAGTTCGTTTGAATTTATCGAGAGCGTTAAATCCGATCTCTTCCCGGATGAGATTTACGTTTTCACACCGGAAGGGCGCATTGTCGAGCTGCCTGCCGGTGCAACGCCCGTCGACt                                <  2:1612647/150‑1 (MQ=255)
                                                                                                                    cAAAGCCTGCTGGAGCTGCAACAGAGCGCCGATAGTTCGTTTGAATTTATCGAGAGCGTTAAATCCGATCTCTTCCCGGATGAGATTTACGTTTTCACACCGGAAGGGCGCATTGTCGAGCTGCCTGCCGGTGCAACGCCCGTCGACTTc                              <  1:3573940/150‑1 (MQ=255)
                                                                                                                     aaaGCCTGCTGGAGCTGCAACAGAGCGCCGATAGTTCGTTTGAATTTATCGAGAGCGTTAAATCCGATCTCTTCCCGGATGAGATTTACGTTTTCACACCGGAAGGGCGCATTGTCGAGCTGCCTGCCGGTGCAACGCCCGTCGACTTCg                             <  1:3997186/150‑1 (MQ=255)
                                                                                                                       aGCCTGCTGGAGCTGCAACAGAGCGCCGATAGTTCGTTTGAATTTATCGAGAGCGTTAAATCCGATCTCTTCCCGGATGAGATTTACGTTTTCACACCGGAAGGGCGCATTGTCGAGCTGCCTGCCGGTGCAACGCCCGTCGACTTCGCt                           >  1:210039/1‑150 (MQ=255)
                                                                                                                       aGCCTGCTGGAGCTGCAACAGAGCGCCGATAGTTCGTTTGAATTTATCGAGAGCGTTAAATCCGATCTCTTCCCGGATGAGATTTACGTTTTCACACCGGAAGGGCGCATTGTCGAGCTGCCTGCCGGTGCAACGCCCGTCGACTTCGCt                           <  1:1167888/150‑1 (MQ=255)
                                                                                                                          ctgctgGAGCTGCAACAGAGCGCCGATAGTTCGTTTGAATTTATCGAGAGCGTTAAATCCGATCTCTTCCCGGATGAGATTTACGTTTTCACACCGGAAGGGCGCATTGTCGAGCTGCCTGCCGGTGCAACGCCCGTCGACTTCGCTTAt                        <  1:952968/150‑1 (MQ=255)
                                                                                                                           tgctgGAGCTGCAACAGAGCGCCGATAGTTCGTTTGAATTTATCGAGAGCGTTAAATCCGATCTCTTCCCGGATGAGATTTACGTTTTCACACCGGAAGGGCGCATTGTCGAGCTGCCTGCCGGTGCAACGCCCGTCGACTTCGCTTATg                       >  1:4316433/1‑150 (MQ=255)
                                                                                                                            gctgGAGCTGCAACAGAGCGCCGATAGTTCGTTTGAATTTATCGAGAGCGTTAAATCCGATCTCTTCCCGGATGAGATTTACGTTTTCACACCGGAAGGGCGCATTGTCGAGCTGCCTGCCGGTGCAACGCCCGTCGACTTCGCTTATGc                      <  2:4525253/150‑1 (MQ=255)
                                                                                                                              tgGAGCTGCAACAGAGCGCCGATAGTTCGTTTGAATTTATCGAGAGCGTTAAATCCGATCTCTTCCCGGATGAGATTTACGTTTTCACACCGGAAGGGCGCATTGTCGAGCTGCCTGCCGGTGCAACGCCCGTCGACTTCGCTTATGCAg                    <  2:3067997/150‑1 (MQ=255)
                                                                                                                               ggAGCTGCAACAGAGCGCCGATAGTTCGTTTGAATTTATCGAGAGCGTTAAATCCGATCTCTTCCCGGATGAGATTTACGTTTTCACACCGGAAGGGCGCATTGTCGAGCTGCCTGCCGGTGCAACGCCCGTCGACTTCGCTTATGCAGt                   >  2:3986070/1‑150 (MQ=255)
                                                                                                                                  gCTGCAACAGAGCGCCGATAGTTCGTTTGAATTTATCGAGAGCGTTAAATCCGATCTCTTCCCGGATGAGATTTACGTTTTCACACCGGAAGGGCGCATTGTCGAGCTGCCTGCCGGTGCAACGCCCGTCGACTTCGCTTATGCAGTGCa                <  1:1083121/150‑1 (MQ=255)
                                                                                                                                  gCTGCAACAGAGCGCCGATAGTTCGTTTGAATTTATCGAGAGCGTTAAATCCGATCTCTTCCCGGATGAGATTTACGTTTTCACACCGGAAGGGCGCATTGTCGAGCTGCCTGCCGGTGCAACGCCCGTCGACTTCGCTTATGCAGTGCa                <  2:2010716/150‑1 (MQ=255)
                                                                                                                                   cTGCAACAGAGCGCCGATAGTTCGTTTGAATTTATCGAGAGCGTTAAATCCGATCTCTTCCCGGATGAGATTTACGTTTTCACACCGGAAGGGCGCATTGTCGAGCTGCCTGCCGGTGCAACGCCCGTCGACTTCGCTTATGCAGTGCAt               <  2:2211137/150‑1 (MQ=255)
                                                                                                                                    tGCAACAGAGCGCCGATAGTTCGTTTGAATTTATCGAGAGCGTTAAATCCCATCTCTTCCCGGATGAGATTTACGTTTTCACACCGGAAGGGCGCCTTGTCGAGCTGCCTGCCGGTGCAACGCCCGTCGACTTCGCTTATGCAGTGCATa              >  1:2068338/1‑150 (MQ=255)
                                                                                                                                      cAACAGAGCGCCGATAGTTCGTTTGAATTTATCGAGAGCGTTAAATCCGATCTCTTCCCGGATGAGATTTACGTTTTCACACCGGAAGGGCGCATTGTCGAGCTGCCTGCCGGTGCAACGCCCGTCGACTTCGCTTATGCAGTGCATAcc            <  2:1957117/150‑1 (MQ=255)
                                                                                                                                       aaCAGAGCGCCGATAGTTCGTTTGAATTTATCGAGAGCGTTAAATCCGATCTCTTCCCGGATGAGATTTACGTTTTCACACCGGAAGGGCGCATTGTCGAGCTGCCTGCCGGTGCAACGCCCGTCGACTTCGCTTATGCAGTGCATACCg           >  1:3151346/1‑150 (MQ=255)
                                                                                                                                       aaCAGAGCGCCGATAGTTCGTTTGAATTTATCGAGAGCGTTAAATCCGATCTCTTCCCGGATGAGATTTACGTTTTCACACCGGAAGGGCGCATTGTCGAGCTGCCTGCCGGTGCAACGCCCGTCGACTTCGCTTATGCAGTGCATACCg           >  1:1269370/1‑150 (MQ=255)
                                                                                                                                          agagCGCCGATAGTTCGTTTGAATTTATCGAGAGCGTTAAATCCGATCTCTTCCCGGATGAGATTTACGTTTTCACACCGGAAGGGCGCATTGTCGAGCTGCCTGCCGGTGCAACGCCCGTCGACTTCGCTTATGCAGTGCATACCGata        <  2:892786/150‑1 (MQ=255)
                                                                                                                                           gagCGCCGATAGTTCGTTTGAATTTATCGAGAGCGTTAAATCCGATCTCTTCCCGGATGAGATTTACGTTTTCACACCGGAAGGGCGCATTGTCGAGCTGCCTGCCGGTGCAACGCCCGTCGACTTCGCTTATGCAGTGCATACCGatat       <  1:382217/150‑1 (MQ=255)
                                                                                                                                            agCGCCGATAGTTCGTTTGAATTTATCGAGAGCGTTAAATCCGATCTCTTCCCGGATGAGATTTACGTTTTCACACCGGAAGGGCGCATTGTCGAGCTGCCTGCCGGTGCAACGCCCGTCGACTTCGCTTATGCAGTGCATACCGATATc      >  1:1861964/1‑150 (MQ=255)
                                                                                                                                            agCGCCGATAGTTCGTTTGAATTTATCGAGAGCGTTAAATCCGATCTCTTCCCGGATGAGATTTACGTTTTCACACCGGAAGGGCGCATTGTCGAGCTGCCTGCCGGTGCAACGCCCGTCGACTTCGCTTATGCAGTGCATACCGATATc      <  1:1586345/150‑1 (MQ=255)
                                                                                                                                             gcgcCGATAGTTCGTTTGAATTTATCGAGAGCGTTAAATCCGATCTCTTCCCGGATGAGATTTACGTTTTCACACCGGAAGGGCGCATTGTCGAGCTGCCTGCCGGTGCAACGCCCGTCGACTTCGCTTATGCAGTGCATACCGATATCg     <  1:2457849/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
AGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGCCGCGCACTGGGCTTATAAAGAGCACGGCGAAACCAGTACTACCGCACAAATCCGCGCCCAGCGCTGGATGCAAAGCCTGCTGGAGCTGCAACAGAGCGCCGGTAGTTCGTTTGAATTTATCGAGAGCGTTAAATCCGATCTCTTCCCGGATGAGATTTACGTTTTCACACCGGAAGGGCGCATTGTCGAGCTGCCTGCCGGTGCAACGCCCGTCGACTTCGCTTATGCAGTGCATACCGATATCGGTC  >  AP012306/3252574‑3252867

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑