BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AP012306	2,073,848	Δ2 bp	intergenic (+106/+161)	lysV → / ← xapR	tRNA‑Lys/DNA‑binding transcriptional activator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AP012306	2,073,847	0	G	.	100.0%	589.6 / NA	127	intergenic (+105/+163)	lysV/xapR	tRNA‑Lys/DNA‑binding transcriptional activator
Reads supporting (aligned to +/- strand): ref base G (0/0); new base . (33/94); total (33/94)
*	AP012306	2,073,848	0	T	.	100.0%	589.6 / NA	127	intergenic (+106/+162)	lysV/xapR	tRNA‑Lys/DNA‑binding transcriptional activator
Reads supporting (aligned to +/- strand): ref base T (0/0); new base . (33/94); total (33/94)

Only 100 of 141 total aligned reads displayed.

CTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGTGCTGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAGACTACTATTT  >  AP012306/2073698‑2073987

||

cTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCg                                                                                                                                              >  2:2954381/1‑150 (MQ=32)

cTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCg                                                                                                                                              >  2:2420190/1‑150 (MQ=32)

cTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCg                                                                                                                                              >  2:1919832/1‑150 (MQ=32)

 ttttAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCgc                                                                                                                                             >  2:3497714/1‑149 (MQ=14)

 ttttAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCgc                                                                                                                                             >  2:4871964/1‑149 (MQ=14)

 ttttAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCgc                                                                                                                                             >  2:3824805/1‑149 (MQ=14)

  tttAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCgcg                                                                                                                                            >  2:822678/1‑148 (MQ=11)

  tttAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCgcg                                                                                                                                            <  2:3485270/150‑3 (MQ=11)

  tttAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCgcg                                                                                                                                            >  1:4578208/1‑148 (MQ=11)

   ttAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCgcgg                                                                                                                                           >  2:4201476/1‑147 (MQ=1)

                                                                                                    gAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGa                                       <  1:1499583/150‑1 (MQ=0)

                                                                                                    gAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGa                                       <  1:4424425/150‑1 (MQ=0)

                                                                                                     aaCCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGaa                                      >  1:3611534/1‑150 (MQ=2)

                                                                                                     aaCCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGaa                                      >  1:1952134/1‑150 (MQ=2)

                                                                                                      aCCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGaaa                                     <  2:3994060/150‑1 (MQ=255)

                                                                                                      aCCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGaaa                                     <  1:3143555/150‑1 (MQ=255)

                                                                                                       ccTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAAt                                    <  1:3213556/150‑1 (MQ=255)

                                                                                                       ccTGCAGCAGGTATGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAAt                                    <  2:256364/150‑1 (MQ=11)

                                                                                                        cTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAAtt                                   >  2:4169728/1‑150 (MQ=255)

                                                                                                        cTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAAtt                                   <  1:3511204/150‑1 (MQ=255)

                                                                                                        cTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAAtt                                   >  1:4175393/1‑150 (MQ=255)

                                                                                                        cTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAAtt                                   <  1:2296172/150‑1 (MQ=255)

                                                                                                        cTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAAtt                                   <  1:2659533/150‑1 (MQ=255)

                                                                                                         tGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTg                                  >  2:290365/1‑150 (MQ=255)

                                                                                                          gcagcaGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGc                                 <  1:1189263/150‑1 (MQ=255)

                                                                                                            agcagGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCt                               >  1:723736/1‑150 (MQ=255)

                                                                                                            agcagGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCt                               >  2:543132/1‑150 (MQ=255)

                                                                                                            agcagGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCt                               <  1:441514/150‑1 (MQ=255)

                                                                                                            agcagGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCt                               <  1:4054431/150‑1 (MQ=255)

                                                                                                            agcagGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCt                               <  2:2156851/150‑1 (MQ=255)

                                                                                                            agcagGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCt                               <  2:4043519/150‑1 (MQ=255)

                                                                                                             gcagGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTg                              >  2:317204/1‑150 (MQ=255)

                                                                                                             gcagGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTg                              <  2:2172703/150‑1 (MQ=255)

                                                                                                               agGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGAt                            <  1:1613336/150‑1 (MQ=255)

                                                                                                               agGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGAt                            <  1:4809730/150‑1 (MQ=255)

                                                                                                               agGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGAt                            >  1:1994933/1‑150 (MQ=255)

                                                                                                               agGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGAt                            <  2:3229135/150‑1 (MQ=255)

                                                                                                               agGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGAt                            <  2:276087/150‑1 (MQ=255)

                                                                                                                 gTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATgc                          >  1:356570/1‑150 (MQ=255)

                                                                                                                 gTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATgc                          <  1:4187111/150‑1 (MQ=255)

                                                                                                                  tttGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATgcg                         <  2:44466/150‑1 (MQ=255)

                                                                                                                  tttGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATgcg                         <  1:2020682/150‑1 (MQ=255)

                                                                                                                  tttGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATgcg                         <  1:4761767/150‑1 (MQ=255)

                                                                                                                   ttgagttgagCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATgcgc                        >  1:2958119/1‑150 (MQ=255)

                                                                                                                     gagttgagCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCtt                      <  1:556676/150‑1 (MQ=255)

                                                                                                                      agttgagCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTc                     >  1:4317687/1‑150 (MQ=255)

                                                                                                                      agttgagCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTc                     <  1:846222/150‑1 (MQ=255)

                                                                                                                       gttgagCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCa                    <  1:1839979/150‑1 (MQ=255)

                                                                                                                       gttgagCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCa                    <  1:4920477/150‑1 (MQ=255)

                                                                                                                        ttgagCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCAc                   >  1:4909112/1‑150 (MQ=255)

                                                                                                                        ttgagCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCAc                   <  1:34886/150‑1 (MQ=255)

                                                                                                                         tgagCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACt                  <  1:3056397/150‑1 (MQ=255)

                                                                                                                          gagCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACtt                 <  2:2417248/150‑1 (MQ=255)

                                                                                                                          gagCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACtt                 <  1:1443381/150‑1 (MQ=255)

                                                                                                                          gagCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACtt                 <  1:4174215/150‑1 (MQ=255)

                                                                                                                           agCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTa                <  1:860470/150‑1 (MQ=255)

                                                                                                                           agCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTa                <  2:1350204/150‑1 (MQ=255)

                                                                                                                            gcgcAGCGAAGCAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAg               <  1:2922155/150‑1 (MQ=17)

                                                                                                                            gcgcAGCGAAACAGCGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAg               <  1:1160609/150‑1 (MQ=17)

                                                                                                                            gcgcAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAg               <  1:2783935/150‑1 (MQ=255)

                                                                                                                             cgcAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGc              <  2:2747126/150‑1 (MQ=255)

                                                                                                                             cgcAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGc              >  1:3550992/1‑150 (MQ=255)

                                                                                                                             cgcAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGc              <  2:4678959/150‑1 (MQ=255)

                                                                                                                              gcAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCa             <  2:1896870/150‑1 (MQ=255)

                                                                                                                              gcAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCa             <  2:3638899/150‑1 (MQ=255)

                                                                                                                              gcAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCa             <  2:2550298/150‑1 (MQ=255)

                                                                                                                              gcAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCa             <  1:1296173/150‑1 (MQ=255)

                                                                                                                              gcAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCa             <  2:813719/150‑1 (MQ=255)

                                                                                                                               cAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAg            <  2:3809965/150‑1 (MQ=255)

                                                                                                                               cAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAg            <  1:1310948/150‑1 (MQ=255)

                                                                                                                               cAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAg            >  1:3018838/1‑150 (MQ=255)

                                                                                                                                aGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAGa           >  2:1698298/1‑150 (MQ=255)

                                                                                                                                aGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAGa           <  1:1643890/150‑1 (MQ=255)

                                                                                                                                 gCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAGac          <  1:671644/150‑1 (MQ=255)

                                                                                                                                  cGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAGact         <  2:39403/150‑1 (MQ=255)

                                                                                                                                  cGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAGact         <  1:2602112/150‑1 (MQ=255)

                                                                                                                                   gAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAGacta        >  1:182417/1‑150 (MQ=255)

                                                                                                                                   gAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAGacta        >  1:4747174/1‑150 (MQ=255)

                                                                                                                                   gAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAGacta        >  1:468897/1‑150 (MQ=255)

                                                                                                                                   gAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAGacta        <  1:3299032/150‑1 (MQ=255)

                                                                                                                                   gAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAGacta        >  2:2561465/1‑150 (MQ=255)

                                                                                                                                    aaaCAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAGactac       <  2:1101796/150‑1 (MQ=255)

                                                                                                                                    aaaCAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAGactac       <  1:1562739/150‑1 (MQ=255)

                                                                                                                                    aaaCAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAGactac       <  1:2256845/150‑1 (MQ=255)

                                                                                                                                     aacaacGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAGactact      <  1:437864/150‑1 (MQ=255)

                                                                                                                                     aacaacGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAGactact      <  2:1741148/150‑1 (MQ=255)

                                                                                                                                     aacaacGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAGactact      <  1:2106740/150‑1 (MQ=255)

                                                                                                                                     aacaacGGAGCAGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTCGCAGactact      >  2:1176373/1‑150 (MQ=17)

                                                                                                                                      acaacGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAGActacta     <  1:1109419/150‑1 (MQ=255)

                                                                                                                                      acaacGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAGActacta     <  1:4570710/150‑1 (MQ=255)

                                                                                                                                      acaacGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAGActacta     <  2:1544660/150‑1 (MQ=255)

                                                                                                                                      acaacGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAGActacta     >  1:393743/1‑150 (MQ=255)

                                                                                                                                       caacGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAGACTACTAt    <  2:2112021/150‑1 (MQ=255)

                                                                                                                                       caacGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAGACTACTAt    <  1:226376/150‑1 (MQ=255)

                                                                                                                                       caacGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAGACTACTAt    <  2:577756/150‑1 (MQ=255)

                                                                                                                                       caacGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAGACTACTAt    <  2:2691877/150‑1 (MQ=255)

                                                                                                                                        aacGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAGACTACTAtt   <  2:13493/150‑1 (MQ=255)

                                                                                                                                        aacGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAGACTACTAtt   <  2:2743080/150‑1 (MQ=255)

                                                                                                                                         acGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAGACTACTAttt  <  2:2635837/150‑1 (MQ=255)

                                                                                                                                         acGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATACTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAGACTACTAttt  <  1:2925319/150‑1 (MQ=18)

||

CTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGTGCTGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAGACTACTATTT  >  AP012306/2073698‑2073987

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`