Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP012306406,6770AG23.1% 672.7 / 24.3 256K294E (AAA→GAA) hemHferrochelatase
Reads supporting (aligned to +/- strand):  ref base A (46/151);  new base G (59/0);  total (105/151)
Fisher's exact test for biased strand distribution p-value = 2.29e-29
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 309 total aligned reads displayed.

TGCCTTATACCGACGAAACGCTGAAAATGCTCGGAGAAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGTTTTCATCATCCGTGAA  >  AP012306/406528‑406826
                                                                                                                                                     |                                                                                                                                                     
tGCCTTATACCGACGAAACGCTGAAAATGCTCGGAGAAAAAGGCGTAGTTCATATTCAGGTGATATGCCCGGGCTTTGCTGCGGATTGACTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTTTTCGTCGGTGCCGGCGGGa                                                                                                                                                       >  2:2981258/1‑150 (MQ=255)
tGCCTTATACCGACGAAACGCTGAAAATGCTCGGAGAAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGa                                                                                                                                                       <  1:2320118/150‑1 (MQ=255)
   cTTATACCGACGAAACGCTGAAAATGCTCGGAGAAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGgaaa                                                                                                                                                    >  1:3663942/1‑150 (MQ=255)
   cTTATACCGACGAAACGCTGAAAATGCTCGGAGAAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGaaaa                                                                                                                                                    <  1:2357047/150‑1 (MQ=255)
       taCCGACGAAACGCTGAAAATGCTCGGAGAAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAata                                                                                                                                                <  2:2022217/150‑1 (MQ=255)
         ccGACGAAACGCTGAAAATGCTCGGAGAAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGgaaaaaaaaa                                                                                                                                              >  2:2384176/1‑146 (MQ=255)
         ccGACGAAACGCTGAAAATGCTCGGAGAAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATg                                                                                                                                              <  1:4493756/150‑1 (MQ=255)
          cgacgaAACGCTGAAAATGCTCGGAGAAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGGAAAAATATGa                                                                                                                                             >  1:4949920/1‑150 (MQ=255)
          cgacgaAACGCTGAAAATGCTCGGAGAAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGa                                                                                                                                             <  1:3348000/150‑1 (MQ=255)
           gacgaAACGCTGAAAATGCTCGGAGAAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGaaaaaaaaataa                                                                                                                                            >  1:1179158/1‑144 (MQ=255)
           gacgaAACGCTGAAAATGCTCGGAGAAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGGAAAAAAATGaa                                                                                                                                            >  2:559105/1‑150 (MQ=255)
            acgaAACGCTGAAAATGCTCGGAGAAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAat                                                                                                                                           <  1:1326755/150‑1 (MQ=255)
            acgaAACGCTGAAAATGCTCGGAGAAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAat                                                                                                                                           <  1:1568427/150‑1 (MQ=255)
            acgaAACGCTGAAAATGCTCGGAGAAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGCCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGGAAAAAAAAGaaa                                                                                                                                           >  2:3035163/1‑149 (MQ=255)
             cgaAACGCTGAAAATGCTCGGAGAAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGGAAAAAAATTaaaa                                                                                                                                          >  1:3835251/1‑148 (MQ=255)
             cgaAACGCTGAAAATGCTCGGAGAAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAata                                                                                                                                          <  1:2158275/150‑1 (MQ=255)
              gaAACGCTGAAAATGCTCGGAGAAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAatat                                                                                                                                         <  2:1243300/150‑1 (MQ=255)
              gaAACGCTGAAAATGCTCGGAGAAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATAAGAatat                                                                                                                                         >  1:4176834/1‑150 (MQ=255)
              gaAACGCTGAAAATGCTCGGAGAAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGGCTTCCTCGGTGCCGGCGGGAAAAAaaatttattt                                                                                                                                         >  2:889465/1‑144 (MQ=255)
                 aCGCTGAAAATGCTCGGAGAAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATAtt                                                                                                                                      >  1:901533/1‑150 (MQ=255)
                 aCGCTGAAAATGCTCGGAGAAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATAtt                                                                                                                                      <  1:3240982/150‑1 (MQ=255)
                   gCTGAAAATGCTCGGAGAAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTcc                                                                                                                                    <  1:1792818/150‑1 (MQ=255)
                   gCTGAAAATGCTCGGAGAAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTcc                                                                                                                                    <  2:1124914/150‑1 (MQ=255)
                     tGAAAATGCTCGGAGAAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGGAAAAAAATGAATATAtttccg                                                                                                                                  >  1:3726832/1‑146 (MQ=255)
                     tGAAAATGCTCGGAGAAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGGAAAAAAAAGAAAAAAttgatg                                                                                                                                  >  2:3565033/1‑146 (MQ=255)
                       aaaaTGCTCGGAGAAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGgcg                                                                                                                                <  2:2989529/150‑1 (MQ=255)
                       aaaaTGCTCGGAGAAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGgcg                                                                                                                                <  2:4680073/150‑1 (MQ=255)
                         aaTGCTCGGAGAAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAAAATGAATATATTTCGGCGCt                                                                                                                              >  1:4850245/1‑150 (MQ=255)
                           tGCTCGGAGAAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGGAAAAATATGAATATATTTCGGCGCTTa                                                                                                                            >  1:4694570/1‑150 (MQ=255)
                           tGCTCGGAGAAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTa                                                                                                                            <  1:2518253/150‑1 (MQ=255)
                              tCGGAGAAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATg                                                                                                                         <  1:5008378/150‑1 (MQ=255)
                              tCGGAGAAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATg                                                                                                                         <  1:1088892/150‑1 (MQ=255)
                                 gagaAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGGAAAAAAAATAAAATATTTCGGCGCTTAATGCCa                                                                                                                      >  2:1606066/1‑150 (MQ=255)
                                  agaAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCAc                                                                                                                     <  2:3106328/150‑1 (MQ=255)
                                     aaaaGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAGAAAAAAAGAATATATTCCGGCGCCTAATgtcccccc                                                                                                                  >  2:4562843/1‑143 (MQ=255)
                                     aaaaGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGcc                                                                                                                  <  1:4107727/150‑1 (MQ=255)
                                       aaGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCgg                                                                                                                <  2:2150349/150‑1 (MQ=255)
                                          gCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGGAAAAATAAGAATATATTCCGGCGCCTTATGGCACGCCGGGAc                                                                                                             >  2:1421200/1‑150 (MQ=255)
                                           cGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGGAAAAAAAATAAAAAATTCCGGGGCTTAATTCCCCGCCCGAACa                                                                                                            >  2:823047/1‑150 (MQ=255)
                                           cGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACa                                                                                                            <  1:145666/150‑1 (MQ=255)
                                            gTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACat                                                                                                           <  1:4735299/150‑1 (MQ=255)
                                             tAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGGACata                                                                                                          >  1:3675504/1‑150 (MQ=255)
                                              aGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACatat                                                                                                         <  1:4367861/150‑1 (MQ=255)
                                              aGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACatat                                                                                                         <  1:1854869/150‑1 (MQ=255)
                                               ggTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATc                                                                                                        <  2:1824722/150‑1 (MQ=255)
                                                  cATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGaa                                                                                                     <  1:1196884/150‑1 (MQ=255)
                                                   atatTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGGAAAAAAATGAATATATTTCGGCGCTTAATTCCACCCCGGGACAAATCGaaa                                                                                                    >  2:2825468/1‑150 (MQ=255)
                                                   atatTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGaaa                                                                                                    <  1:3113125/150‑1 (MQ=255)
                                                    tatTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGGAAAAATATGAATATATTTCGGCGCTTAATGCCACGCCGGGACATATCGAAat                                                                                                   >  1:2258642/1‑150 (MQ=255)
                                                      ttCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGGAAAAATATTAATATATTCCGGCGCTTTATGCCACGCCGGGACATATCGAAaaga                                                                                                 >  1:3609543/1‑150 (MQ=255)
                                                      ttCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAatga                                                                                                 <  2:1052715/150‑1 (MQ=255)
                                                       tCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAatgat                                                                                                <  1:1430707/150‑1 (MQ=255)
                                                         aGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGGAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGGACATATCGAAATGATgg                                                                                              >  1:104677/1‑150 (MQ=255)
                                                         aGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATgg                                                                                              <  1:4919075/150‑1 (MQ=255)
                                                         aGGTGATGTGCCCGGGCTTTGCTGCGGATTGGCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATgg                                                                                              <  2:1680210/150‑1 (MQ=255)
                                                          ggTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTTCGGCGCTTTATTACCCGCCGGGACAAATTGAAATGAAggg                                                                                             >  2:2097167/1‑149 (MQ=255)
                                                           gTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCt                                                                                            <  1:392680/150‑1 (MQ=255)
                                                           gTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCCGCGCCTAATGCCACGCCCGGACATATCGGAATGATGGCt                                                                                            >  1:992516/1‑150 (MQ=255)
                                                             gATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGGAAAAAAATGAATATATTCCGGCGCCTTATGCCACGCCGGGACATATCGAAATGATGGGTaa                                                                                          >  1:66669/1‑150 (MQ=255)
                                                               tgtgCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGGAAAAATATGAAAATATTTCGGCGCTTAATGCCACGCCGGGACATATCGAAATGATGGCTaaac                                                                                        >  2:2313458/1‑148 (MQ=255)
                                                                 tgCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCtt                                                                                      <  2:390830/150‑1 (MQ=255)
                                                                  gCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCttg                                                                                     <  1:3503497/150‑1 (MQ=255)
                                                                   cccGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCttgt                                                                                    <  1:1244274/150‑1 (MQ=255)
                                                                       ggCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGcc                                                                                <  2:3867360/150‑1 (MQ=255)
                                                                        gCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCcg                                                                               <  2:4261638/150‑1 (MQ=255)
                                                                         cTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGGAAAAATATGAAAATATTTCGGCGCTTAATGCCACCCCGGGACATATCGAAAAAATGGCTAATATTTTTGCcgc                                                                              >  2:2719694/1‑150 (MQ=255)
                                                                          tttGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAAGATAAAAATATTCCCGCGCCTAATGCCACCCCCGGAAAAATCGAAAAGAAGGCTAATCtttttttcccg                                                                             >  2:3210447/1‑144 (MQ=255)
                                                                           ttGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGt                                                                            <  1:2054650/150‑1 (MQ=255)
                                                                             gctgcGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGGAAAAAAATGAATATATTCCGGCGCTTAATTCCCCGCCCGAACATATCGAAATGATGGGTAATCTTGTTTGCGCGTAt                                                                          >  2:286294/1‑150 (MQ=255)
                                                                             gctgcGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTAt                                                                          <  2:3412417/150‑1 (MQ=255)
                                                                               tgcGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGGAAAAAAAAGAATAAAATCCGGCGCCTTATTCCCCCCCCGAACATATCGAAAAGATGGGTAAACTTTTTTCCCCCTATCg                                                                        >  2:2262542/1‑150 (MQ=255)
                                                                                 cGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCt                                                                      <  2:4892565/150‑1 (MQ=255)
                                                                                  ggATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGGAAAAAAATCAATATATTTCGGCGCCTGATGCCCCGCCGGGACATAACGAAATGAAGGCGAATCTTTTTTCCGCGCATCGcca                                                                     >  2:2959436/1‑148 (MQ=255)
                                                                                    aTTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTaaa                                                                   <  1:3438445/150‑1 (MQ=255)
                                                                                     ttGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAg                                                                  <  1:2083343/150‑1 (MQ=255)
                                                                                        tCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTg                                                               <  1:861679/150‑1 (MQ=255)
                                                                                          tGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAg                                                             <  1:4473522/150‑1 (MQ=255)
                                                                                           ggAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGc                                                            <  2:3472422/150‑1 (MQ=255)
                                                                                           ggAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAAAATGAAAATATTTCGGCGCTTAATGCCACGCCGGGACATATCGAAATGATTGCTAATCTTGTTTCCGCGGATCGCTAAAACTGAGc                                                            >  2:61031/1‑150 (MQ=255)
                                                                                               aCGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTa                                                        <  1:2812273/150‑1 (MQ=255)
                                                                                               aCGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTa                                                        <  1:1601865/150‑1 (MQ=255)
                                                                                                 gCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGGAAAAAAATGAAAATATTTCGGGGCCTTAAGCCACGCCCGGAAAAATCGAAAAGATGGGTAAACTTTTTTCCGCGTATCGCTAAAACTGAGGGGGaaa                                                      >  2:3718653/1‑150 (MQ=255)
                                                                                                  cTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAg                                                     >  1:3288478/1‑150 (MQ=255)
                                                                                                  cTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAg                                                     <  2:2238150/150‑1 (MQ=255)
                                                                                                   tGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGa                                                    <  1:4636792/150‑1 (MQ=255)
                                                                                                   tGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGa                                                    <  2:1880343/150‑1 (MQ=255)
                                                                                                     gAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAAAATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGGAAAGAAc                                                  >  1:455956/1‑150 (MQ=255)
                                                                                                       agagATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGGAAAAAAATGAATAAATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAACTGAGCGGTAAAAAACTg                                                >  2:4027580/1‑150 (MQ=255)
                                                                                                       agagATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTg                                                <  1:4055037/150‑1 (MQ=255)
                                                                                                          gaTTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAgc                                             <  1:3889464/150‑1 (MQ=255)
                                                                                                          gaTTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAgc                                             <  2:4435008/150‑1 (MQ=255)
                                                                                                             tGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGcc                                          <  2:4482275/150‑1 (MQ=255)
                                                                                                                             gAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCtcg                          >  1:4247718/1‑150 (MQ=255)
                                                                                                                                gTCTTCCTCGGTGCCGGCGGGGAAAAAAATTAAAATATTTCTGGGCTTAATTGCACGCCCGGCCATATCGGAAAGATGGCTAATCTTGTTTCCGCGTATCGCCAAAAATGAGCGGGTAAAAACAGAGGGCCGCCGCGAAGAACCccgtcc                       >  2:3624577/1‑149 (MQ=255)
                                                                                                                                gTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCtcgtcg                       <  2:159188/150‑1 (MQ=255)
                                                                                                                                      cTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGtt                 <  2:3804985/150‑1 (MQ=255)
                                                                                                                                       tCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGttt                <  2:458910/150‑1 (MQ=255)
                                                                                                                                       tCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGttt                <  2:481069/150‑1 (MQ=255)
                                                                                                                                                gcggGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGTTTTCATCATcc       <  1:3069709/150‑1 (MQ=255)
                                                                                                                                                   ggAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGTTTTCATCATCCGTg    <  2:4121348/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TGCCTTATACCGACGAAACGCTGAAAATGCTCGGAGAAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGTTTTCATCATCCGTGAA  >  AP012306/406528‑406826

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑