Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123061,944,2880AC32.2% 485.1 / 53.3 237G361G (GGT→GGG) menEo‑succinylbenzoate‑CoA ligase
Reads supporting (aligned to +/- strand):  ref base A (109/51);  new base C (0/76);  total (109/128)
Fisher's exact test for biased strand distribution p-value = 7.49e-28
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 309 total aligned reads displayed.

ACCCATTCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATA‑CCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCTTCGTCATTAACCAGTGAAACCAGTTGCCCGTTACGCCAGTAACCTTCTGC  >  AP012306/1944139‑1944434
                                                                                                                                                     |                                                                                                                                                   
aCCCATTCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATa                                                                                                                                                     >  2:2032757/1‑150 (MQ=255)
aCCCATTCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATa                                                                                                                                                     >  2:4115360/1‑150 (MQ=255)
   cATTCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATA‑ccc                                                                                                                                                 <  1:2698521/150‑1 (MQ=255)
   cATTCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATA‑ccc                                                                                                                                                 <  2:870207/150‑1 (MQ=255)
    aTTCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATA‑CCct                                                                                                                                                <  1:486510/150‑1 (MQ=255)
     ttCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATA‑CCctc                                                                                                                                               <  1:2594654/150‑1 (MQ=255)
     ttCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATA‑CCctc                                                                                                                                               <  2:4677495/150‑1 (MQ=255)
      tCACTAAGATCAACGCTCTCGTGGTCATACTCCTTCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATA‑CCctct                                                                                                                                              <  1:2019810/150‑1 (MQ=255)
       cACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATA‑CCCtctc                                                                                                                                             <  2:127050/150‑1 (MQ=255)
       cACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATA‑CCCtctc                                                                                                                                             >  1:2987703/1‑150 (MQ=255)
        aCTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATA‑CCCTCTcc                                                                                                                                            <  2:2283135/150‑1 (MQ=255)
        aCTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATA‑CCCTCTcc                                                                                                                                            >  1:4151351/1‑150 (MQ=255)
           aaGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATA‑CCCTCTCCGcc                                                                                                                                         >  1:4660914/1‑150 (MQ=255)
            aGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATA‑CCCTCTCCGCCa                                                                                                                                        <  1:380393/150‑1 (MQ=255)
                cAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAACCCCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATC‑CCCTCTCCGCCACTga                                                                                                                                    <  1:63427/150‑1 (MQ=255)
                cAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATA‑CCCTCTCCGCCACTga                                                                                                                                    >  2:1571360/1‑150 (MQ=255)
                cAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATA‑CCCTCTCCGCCACTga                                                                                                                                    >  2:4764382/1‑150 (MQ=255)
                 aaCGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATA‑CCCTCTCCGCCACTgaa                                                                                                                                   >  1:3247977/1‑150 (MQ=255)
                    gCTCTCGGGTTCATACTCCATCCCCGCCACCGCTCGATGCCCAACCTCCTTGTCGGCAACGGGGACGATAAACCCCTGCAAAACCGCAGGATGTGCACCAATTACGCGCTCGACTTCTTCCGGCTGAATC‑CCCTCTCCGCCACTgaagaa                                                                                                                                <  2:1337544/150‑1 (MQ=255)
                     ctctCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCACCGGGGACGATAAACACCTGAAAACCCGCAGGATGTGCAGCAATTACGCGCTCGATTTCTTCCGGCTGAATC‑CCCTCTCCGCCACTGAAGAAt                                                                                                                               <  2:2444215/150‑1 (MQ=255)
                         cgggctCACACTCGATCCCCCCCACCGCTCGATGCCCAATCTCATTGTCGACAACGGGGAGGTGAAACCCCTCAAAAACCGCAGCATGTGCAACATTTACGCCCCGGTTTTCTTCCGGTTAAATC‑CCCTCTCCGCCACTGAAGAATAGAt                                                                                                                           <  2:3300074/145‑1 (MQ=255)
                           tGGTCATCCCCCACCACCGCCCCCGGGCGATGACCAAACTCCTTGTCGGCAACGGGGAAGAAAAACCCCTGCAAACCCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTAAATC‑CCCTCTCCGCCACTGAAGAATAGATTg                                                                                                                         <  2:4480032/150‑1 (MQ=255)
                            gttCATACTCCTTCCCCGCCCCCGTTCGATGCCCAAACCCCTTGTCGGCAACGGGGACGAAAAACCCCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCAGAATC‑CCCTCTCCGCCACTGAAGAATAGATTGt                                                                                                                        <  2:907402/148‑1 (MQ=255)
                             gTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATA‑CCCTCTCCGCCACTGAAGAATAGATTGTc                                                                                                                       <  1:2805211/150‑1 (MQ=255)
                               cATCCTCCATCCCCGCCACCGGTCGATGACCAACCTCCTTGTCGGCAACGGGGACGATAACCACCTGCAAAACCGCAGGATGTGCAGCAATTACGCCCTCGCCTTCTTCCGGCTGAATC‑CCCTCTCCGCCACTGAAGAATAGATTGTCTa                                                                                                                     <  2:181065/150‑1 (MQ=255)
                               cATCCTCCATCCCCGCCACCGGTCGATGACCAACCTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAACCCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATC‑CCCTCTCCGCCACTGAAGAATAGATTGTCTa                                                                                                                     <  2:30157/150‑1 (MQ=255)
                               cATACTCCACCACCGCCACCGGTCGATGACCAACCTCCTTGTCGGCAACGGGGACGATAAACCCCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGCCTTCTTCCGGCTGAATC‑CCCTCTCCGCCACTGAAGAATAGATTGTCTa                                                                                                                     <  2:4413860/150‑1 (MQ=255)
                               aaTACTCCATCACCGCCACCGGCCGATGAACAAACTCCTTGTCAGCAACCGGTACGATAAACACCTGCAAAACCGCACGATGTGAAGCAAACACGCGCTCGACTTCTCCCGCCTGAATA‑CCCTCTCCGCCACTGAAGAATAGATTGTCTa                                                                                                                     >  2:2142649/2‑150 (MQ=255)
                                aTACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATA‑CCCTCTCCGCCACTGAAGAATAGATTGTCTaa                                                                                                                    >  2:4976563/1‑150 (MQ=255)
                                 tACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATA‑CCCTCTCCGCCACTGAAGAATAGATTGTCTaaa                                                                                                                   <  1:691539/150‑1 (MQ=255)
                                  ccTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATC‑CCCTCTCCGCCACTGAAGAATAGATTGTCTAAAc                                                                                                                  <  1:2505521/149‑1 (MQ=255)
                                  aCTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATA‑CCCTCTCCGCCACTGAAGAATAGATTGTCTAAAc                                                                                                                  <  1:4960450/150‑1 (MQ=255)
                                  aCTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATA‑CCCTCTCCGCCACTGAAGAATAGATTGTCTAAAc                                                                                                                  >  2:1101087/1‑150 (MQ=255)
                                    tcaaTCCCCGCCACCGGTCGATGACCAACCTCCTTGTCGGCAACGGGGACAATAAACCCCTGCAAAACCGCAGGATGTGCAGCAATTACGCCCTCGTCTTCTTCCGGGTGAATC‑CCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGt                                                                                                                <  2:3134735/147‑1 (MQ=255)
                                    tCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATA‑CCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGt                                                                                                                >  1:1572826/1‑150 (MQ=255)
                                    cccacccccGCCACCGGGCGATGCCCAAACTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATA‑CCCCCTCCGCCACTGAAGAATAGATTGTCTAAACGt                                                                                                                <  2:2663311/143‑1 (MQ=255)
                                     ccATCACCGCCACCGGTCGATGACAAAACTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATA‑CCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTc                                                                                                               <  1:4400944/150‑1 (MQ=255)
                                        tCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATC‑CCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGa                                                                                                            <  1:101788/150‑1 (MQ=255)
                                         ccccGCCACCGGTCGATGACCAACCCCCTTGTCGGCAACGGGGACGATAAACCCCTCCAAACCCGCAGGATGTGCAGCATTTCCGCGCTCGACTTCTCCCGGCTGAATC‑CCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGAc                                                                                                           <  2:2938810/148‑1 (MQ=255)
                                             gCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAACCCCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATC‑CCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATg                                                                                                       <  1:2267267/150‑1 (MQ=255)
                                              ccACCGGTCGAGGCCCAACCTCCTTGTCGCCAACGGGAACTATAACCCCCTGTAAAGCCGCAATATGTGCAAAAATTACGCTCCCCCCTTCTTCCGGTTGAATC‑CCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATgg                                                                                                      <  2:458147/150‑1 (MQ=255)
                                               cACCGCTCAATGACAAACCCCCTTGTCGGCAACGGGGACGATAAACTCCTGCAAAACCGCAGGAGGTGAAGCATTTACCCGTTGGTCTTCTTCCGGCCGAATC‑CCCTCCCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGt                                                                                                     <  2:2601134/150‑1 (MQ=255)
                                                aCCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATC‑CCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTc                                                                                                    <  1:1442901/150‑1 (MQ=255)
                                                      cGATGACCAACCTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAACCCGCAGGATGTGCAGCAATTCCGCGCTCGACTTCTTCCGGCTAAATA‑CCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTg                                                                                              <  2:1048545/150‑1 (MQ=255)
                                                         tGACCAAACTCCTTGTCGGCAACGGGGACGATAAACCCCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATC‑CCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCa                                                                                           <  2:4034672/150‑1 (MQ=255)
                                                           aCCAACCCCCTTGTCGGCAACGGGGACGATAAACCCCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGTCTTCTTCCGGCTGAATC‑CCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCAtt                                                                                         <  2:660072/150‑1 (MQ=255)
                                                           aCCAAACTCCTTGTCGGCAACGGGGACGATAAACCCCTGCAAAACCGCAGGATGGGCAGCAATTACGCGCTCGATTTCTTCCGGCTGAATC‑CCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCAtt                                                                                         <  2:4738414/150‑1 (MQ=255)
                                                             cAAACTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATA‑CCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTAt                                                                                       <  1:883419/150‑1 (MQ=255)
                                                               aaCTCCTTGTCGGCAACGGGGACGATAAACCCCTGCAAAACCGCAGGATGTGCAGCAATTCCGCGCTCGACTTCTCCCGGCTGAATC‑CCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGc                                                                                     <  2:812223/150‑1 (MQ=255)
                                                                        tCGGCAACGGGGACGATATACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGATTTCTTCCGGCTGAATC‑CCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACcg                                                                            <  2:4367258/150‑1 (MQ=255)
                                                                        tCGGCAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATA‑CCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACcg                                                                            >  2:2504793/1‑150 (MQ=255)
                                                                        gcGGCACCGGGGCCGATAACCACTTGCAAAACCGCAGAATGTGCAGCATTTACGCGCTCGACTTCTTCCGGCTGAATC‑CCCTGTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACcg                                                                            <  2:3274067/149‑1 (MQ=255)
                                                                          ggCAACGGGGACGATAAACCCCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATC‑CCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACcgcg                                                                          <  1:4311593/150‑1 (MQ=255)
                                                                          ggCAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATC‑CCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACcgcg                                                                          <  1:2344785/150‑1 (MQ=255)
                                                                            cAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATA‑CCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGAt                                                                        >  1:4094714/1‑150 (MQ=255)
                                                                                ggggACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATC‑CCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATcgcg                                                                    <  1:2871368/150‑1 (MQ=255)
                                                                                     cGATAAACCCCTACAAACCCGCAGGATGGGCAGCAGTTACGCGCTCGCCTTCTCCCGGCTGAATC‑CCCCCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCgcgtag                                                               <  2:3884537/150‑1 (MQ=255)
                                                                                     cGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATC‑CCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCgcgtag                                                               <  1:3178280/150‑1 (MQ=255)
                                                                                      gATAAACCCCTACAAAACCGCAGGATGTCCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATC‑CCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCgcgtagc                                                              <  2:1650552/150‑1 (MQ=255)
                                                                                          aaCACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATA‑CCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTAc                                                          >  1:4561165/1‑150 (MQ=255)
                                                                                            cacCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATA‑CCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCa                                                        >  2:1866941/1‑150 (MQ=255)
                                                                                             acCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATA‑CCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAg                                                       >  1:2402053/1‑150 (MQ=255)
                                                                                              ccTGCAAACCCGCAGGATGTGCAGCAATTACGCGATCGATTTCTTCCGGCTGAATC‑CCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGc                                                      <  1:2432215/150‑1 (MQ=255)
                                                                                               cTGCAAACCCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTCCCGGCTGAATC‑CCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGcc                                                     <  2:3961164/150‑1 (MQ=255)
                                                                                                tgaaaaaCCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATC‑CCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCt                                                    <  1:3650119/147‑1 (MQ=255)
                                                                                                tGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATA‑CCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCt                                                    >  1:1873137/1‑150 (MQ=255)
                                                                                                 gCAAACCCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATC‑CCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCtt                                                   <  1:3746271/150‑1 (MQ=255)
                                                                                                 gCAAAACCGCAGGATGGGCAGAAATTCCCCGCTCGGCTTCTCCCGG‑TGAAAACCCCCCCCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCtt                                                   <  2:215805/150‑1 (MQ=255)
                                                                                                   aaaaCCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATA‑CCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCTTCg                                                 >  1:724054/1‑150 (MQ=255)
                                                                                                     aaCCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATC‑CCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCTTCGTc                                               <  1:1734785/150‑1 (MQ=255)
                                                                                                     aaCCGAAGGAGGTCCAGCAATTCCGCCCCCGATTTTTTCCGCCTGAATC‑CCCTCACCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCTTCGTc                                               <  2:4613524/150‑1 (MQ=255)
                                                                                                       ccGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATA‑CCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCTTCGTCAt                                             >  1:1207748/1‑150 (MQ=255)
                                                                                                        cGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATA‑CCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCTTCGTCAtt                                            <  1:3370975/150‑1 (MQ=255)
                                                                                                        cGCAGGATGTGAAGCAATTACGCGCTCGACTTCTTCCGGCTGAATC‑CCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCTTCGTCAtt                                            <  2:1465295/150‑1 (MQ=255)
                                                                                                             gATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATA‑CCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCTTCGTCATTAACCa                                       >  1:572375/1‑150 (MQ=255)
                                                                                                              aTGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATA‑CCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCTTCGTCATTAACCAg                                      >  2:2972521/1‑150 (MQ=255)
                                                                                                                gtgCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATA‑CCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCTTCGTCATTAACCAGTg                                    >  1:1332207/1‑150 (MQ=255)
                                                                                                                   cagcaATTACGCGCTCGACTTCTTCCGGCTGAATA‑CCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCTTCGTCATTAACCAGTGaaa                                 >  1:4229640/1‑150 (MQ=255)
                                                                                                                   cagcaATTACGCGCTCGACTTCTTCCGGCTGAATA‑CCCTCCCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCTTCGTCATTAACCAGTGaaa                                 >  2:769357/1‑150 (MQ=255)
                                                                                                                    agcaATTACGCGCTCGACTTCTTCCGGCTGAATA‑CCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCTTCGTCATTAACCAGTGAAAc                                >  2:4863594/1‑150 (MQ=255)
                                                                                                                     gcaATTACGCGCTCGACTTCTTCCGGCTGAATA‑CCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCTTCGTCATTAACCAGTGAAAcc                               <  1:2665558/150‑1 (MQ=255)
                                                                                                                      caATTACGCGCTCGACTTCTTCCGGCTGAATA‑CCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCTTCGTCATTAACCAGTGAAACCa                              >  1:1152892/1‑150 (MQ=255)
                                                                                                                      caATTACGCGCTCGACTTCTTCCGGCTGAATA‑CCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCTTCGTCATTAACCAGTGAAACCa                              >  1:3882662/1‑150 (MQ=255)
                                                                                                                      aaaTTACGCGGCGGACTTTTTCGGCCGGAATC‑CCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCTTCGTCATTAACCAGTGAAACCa                              <  1:1086427/149‑1 (MQ=255)
                                                                                                                        aTTACGCGCTCGACTTCTTCCGGCTGAATA‑CCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCTTCGTCATTAACCAGTGAAACCAGt                            >  2:2785313/1‑150 (MQ=255)
                                                                                                                           aCGCGCTCGACTTCTTCCGGCTGAATC‑CCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCTTCGTCATTAACCAGTGAAACCAGTTGc                         <  2:3737301/150‑1 (MQ=255)
                                                                                                                           aCGCGCTCGACTTCTTCCGGCTGAATA‑CCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCTTCGTCATTAACCAGTGAAACCAGTTGc                         >  1:4813263/1‑150 (MQ=255)
                                                                                                                             gcgcTCGACTTCTTCCGGCTGAATA‑CCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCTTCGTCATTAACCAGTGAAACCAGTTGccc                       >  1:4043441/1‑150 (MQ=255)
                                                                                                                              cgcTCGCCTTCTTCCGGCTGAATC‑CCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCTTCGTCATTAACCAGTGAAACCAGTTGCCCg                      <  2:2848041/150‑1 (MQ=255)
                                                                                                                              cgcTCGACTTCTTCCGGCTGAATC‑CCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCTTCGTCATTAACCAGTGAAACCAGTTGCCCg                      <  1:1666026/150‑1 (MQ=255)
                                                                                                                              cgcTCGACTTCTTCCGGCTGAATA‑CCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCTTCGTCATTAACCAGTGAAACCAGTTGCCCg                      >  1:1516502/1‑150 (MQ=255)
                                                                                                                                cTCGACTTCTTCCGGCTGAATA‑CCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCTTCGTCATTAACCAGTGAAACCAGTTGCCCGtt                    >  2:3179297/1‑150 (MQ=255)
                                                                                                                                  cGACTTCTTCCGGCTGAATC‑CCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCTTCGTCATTAACCAGTGAAACCAGTTGCCCGTTAc                  <  2:4078024/150‑1 (MQ=255)
                                                                                                                                     tttctacCGACTGAATC‑CCCTCCCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCTTCGTCATTAACCAGTGAAACCAGTTGCCCGTTACGcc               <  1:2204398/149‑1 (MQ=255)
                                                                                                                                      ttcttcCGGCTGAATC‑CCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCTTCGTCATTAACCAGTGAAACCAGTTGCCCGTTACGCCa              <  2:3592756/150‑1 (MQ=255)
                                                                                                                                             ggCTTAATA‑CCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCTTCGTCATTAACCAGTGAAACCAGTTGCCCGTTACGCCAGTAACCt       >  1:3145541/1‑150 (MQ=255)
                                                                                                                                              gCTGAATA‑CCCTCTACGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCTTCGTCATTAACCAGTGAAACCAGTTGCCCGTTACGCCAGTAACCtt      <  2:4255577/150‑1 (MQ=255)
                                                                                                                                               cTGAATC‑CCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCTTCGTCATTAACCAGTGAAACCAGTTGCCCGTTACGCCAGTAACCTTc     <  2:3443083/150‑1 (MQ=255)
                                                                                                                                                tGAATC‑CCGTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCTTCGTCATTAACCAGTGAAACCAGTTGCCCGTTACGCCAGTAACCTTCt    <  2:597351/150‑1 (MQ=255)
                                                                                                                                                tGAATC‑CCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCTTCGTCATTAACCAGTGAAACCAGTTGCCCGTTACGCCAGTAACCTTCt    <  1:2223147/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
ACCCATTCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATA‑CCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCTTCGTCATTAACCAGTGAAACCAGTTGCCCGTTACGCCAGTAACCTTCTGC  >  AP012306/1944139‑1944434

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑