Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123063,020,6700TG28.4% 427.3 / 14.3 216K195N (AAA→AAC) glgCglucose‑1‑phosphate adenylyltransferase
Reads supporting (aligned to +/- strand):  ref base T (97/57);  new base G (61/0);  total (159/57)
Fisher's exact test for biased strand distribution p-value = 1.93e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 314 total aligned reads displayed.

ATCAAATCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGC‑GGGTTAG‑CAGGTTTTTCAACG‑AATT‑CGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGATAAGCATACGCGAGTAGTCTTGCTT  >  AP012306/3020521‑3020819
                                                                                                                                                       |                                                                                                                                                       
 tCAAATCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGC‑GGGTTAG‑CAGGtt                                                                                                                                                        <  2:1276968/150‑1 (MQ=255)
  cAAATCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGC‑GGGTTAG‑CAGGttt                                                                                                                                                       <  1:3459254/150‑1 (MQ=255)
  cAAATCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGC‑GGGTTAG‑CAGGttt                                                                                                                                                       <  2:3834679/150‑1 (MQ=255)
   aaaTCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGC‑GGGTTAG‑CAGGgttt                                                                                                                                                      >  2:4570322/1‑150 (MQ=255)
    aaTCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGC‑GGGGTTG‑CAGGgtttt                                                                                                                                                     >  1:4670982/1‑150 (MQ=255)
     aTCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGC‑GGGTTAG‑CAGGTTTTTc                                                                                                                                                    >  1:2994007/1‑150 (MQ=255)
     aTCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGC‑GGGTTAG‑CAGGTTTTTc                                                                                                                                                    <  2:4104493/150‑1 (MQ=255)
        tttGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGC‑GGGTTAG‑CAGGTTTTTCAAc                                                                                                                                                 <  1:694741/150‑1 (MQ=255)
         ttGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGC‑GGGTTAG‑CAGGGTTTTCAACg                                                                                                                                                >  1:3330867/1‑150 (MQ=255)
           gCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGC‑GGGTTAG‑CAGGTTTTTCAACG‑aa                                                                                                                                             <  1:4100463/150‑1 (MQ=255)
            ccAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGC‑GGGTTAG‑CAGGTTTTTCAACG‑AAt                                                                                                                                            <  2:2301219/150‑1 (MQ=255)
            ccAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGC‑GGGTTAG‑CAGGTTTTTCAACG‑AAt                                                                                                                                            <  2:431994/150‑1 (MQ=255)
            ccAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGC‑GGGTTAG‑CAGGTTTTTCAACG‑AAt                                                                                                                                            <  2:3668671/150‑1 (MQ=255)
             cAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGC‑GGGTTAG‑CAGGTTTTTCAACG‑AAtt                                                                                                                                           <  2:4922714/150‑1 (MQ=255)
             cAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGC‑GGGGTTG‑GAGGGTTTTCAACG‑CAtt                                                                                                                                           >  1:1377700/1‑150 (MQ=255)
              aaaGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGC‑GGGTTAG‑CAGGTTTTTCAACG‑AATT‑c                                                                                                                                         <  1:866921/150‑1 (MQ=255)
              aaaGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGC‑GGGGTAG‑CAGGGTTTTTAAAG‑AATT‑c                                                                                                                                         >  2:94668/1‑150 (MQ=255)
               aaGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGC‑GGGTTAG‑CAGGTTTTTCAACG‑AATT‑Cg                                                                                                                                        <  2:4693722/150‑1 (MQ=255)
                 gTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGGTTAC‑CAGGTTTTTCACCC‑AATT‑CGa                                                                                                                                       >  2:1230824/1‑150 (MQ=255)
                   cGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGC‑GGGTTAG‑CAGGGTTTTCAACG‑AAAT‑TGATaa                                                                                                                                    >  2:2202739/1‑150 (MQ=255)
                    gTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGC‑GGGGTTG‑GAGGGTTTTTCACG‑AATT‑TGATaaa                                                                                                                                   >  1:3700489/1‑149 (MQ=255)
                    gTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGC‑GGGGTAG‑TAAGGTTTTCAACG‑AATT‑TGATaaa                                                                                                                                   >  2:4845769/1‑149 (MQ=255)
                      ggCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGC‑GGGTTAG‑CAGGTTTTTCAACG‑AATT‑CGATAAttt                                                                                                                                 <  1:3566356/150‑1 (MQ=255)
                       gCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGC‑GGGTTAG‑CAGGTTTTTCAACG‑AATT‑CGATAAtttt                                                                                                                                <  1:1048596/150‑1 (MQ=255)
                         tGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGC‑GGGTTAG‑CAGGTTTTTCAACG‑AATT‑CGATAATTTTAt                                                                                                                              <  1:2304838/150‑1 (MQ=255)
                         tGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGC‑GGGTTAG‑CAGGTTTTTCAACG‑AATT‑CGATAATTTTAt                                                                                                                              >  1:1317724/1‑150 (MQ=255)
                         tGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGC‑GGGTTAG‑CAGGTTTTTCAACG‑AATT‑CGATAATTTTAt                                                                                                                              <  2:1833664/150‑1 (MQ=255)
                             gTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGC‑GGGTTAG‑CAGGTTTTTCAACG‑AATT‑CGATAATTTTATCGtt                                                                                                                          <  1:3360231/150‑1 (MQ=255)
                             gTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGC‑GGGGTAG‑CAGGTTTTTCAACG‑AATT‑CGATAATTTTATCGtt                                                                                                                          >  2:146488/1‑150 (MQ=255)
                                ctcATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGC‑GGGTTAG‑CAGGTTTTTCAACG‑AATT‑CGATAATTTTATCGTtctc                                                                                                                       <  2:2328720/150‑1 (MQ=255)
                                  cATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGC‑GGGTTAG‑CAGGTTTTTCAACG‑AATT‑CGATAATTTTATCGTTCtcat                                                                                                                     <  2:3547900/150‑1 (MQ=255)
                                   aTCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGC‑GGGTTAG‑CCGGTTTTTTCACG‑AATT‑CGATAATTTTATCGTTCtcatc                                                                                                                    >  2:2062168/1‑150 (MQ=255)
                                      gcgATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGC‑GGGTTAG‑CAGGTTTTTCAACG‑AATT‑CGATAATTTTATCGTTCTCATCAAc                                                                                                                 <  1:1399904/150‑1 (MQ=255)
                                      gcgATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGGTCGGCATTGACGGC‑GGGGTAA‑CAGGTTTTTCAACC‑AATT‑CGATAATTTTTTCGCTTTCATCaat                                                                                                                 >  2:3875206/1‑149 (MQ=255)
                                       cgATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGC‑GGGTTAG‑CAGGTTTTTTAACG‑AATT‑CGATAATTTTATCGTTCTCATCAAcc                                                                                                                >  2:2951510/1‑150 (MQ=255)
                                       cgATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGC‑GGGTTAG‑CAGGTTTTTCAACG‑AATT‑CGATAATTTTATCGTTCTCATCAAcc                                                                                                                >  1:2494194/1‑150 (MQ=255)
                                        gATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGC‑GGGTTAG‑CAGGTTTTTCAACG‑AATT‑CGATAATTTTATCGTTCTCATCAACCg                                                                                                               <  2:690407/150‑1 (MQ=255)
                                        gATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGC‑GGGTTAG‑CAGGTTTTTCAACG‑AATT‑CGATAATTTTATCGTTCTCATCAACCg                                                                                                               <  2:266686/150‑1 (MQ=255)
                                        gATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGC‑GGGTTAG‑CAGGCTTTTTCACG‑AATT‑CGGTAATTTTTTTGTTCTCATCAACCg                                                                                                               >  2:4176215/1‑150 (MQ=255)
                                        gATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCGCGGATCGTTCGGCATTGACGGC‑GGGGTAG‑CAGGGTTTTCAACG‑AATT‑CGATAATTTTATCGTTCTCATCAACCg                                                                                                               >  2:116028/1‑150 (MQ=255)
                                         aTCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGC‑GGGTTAG‑CAGGTTTTTCAACG‑AATT‑CGATAATTTTATCGTTCTCATCAACCGc                                                                                                              <  1:1759726/150‑1 (MQ=255)
                                           cGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGC‑GGGTTAG‑CAGGTTTTTCAACG‑AATT‑CGATAATTTTATCGTTCTCATCAACCGCCa                                                                                                            <  1:2796802/150‑1 (MQ=255)
                                             tcttctTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGC‑GGGTTAG‑CAGGTTTTTCAACG‑AATT‑CGATAATTTTATCGTTCTCATCAACCGCCATa                                                                                                          >  1:3130494/1‑150 (MQ=255)
                                             tcttctTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGC‑GGGTTAG‑CAGGGTTTTCAACG‑AATT‑CGATAATTTTATCGTTCTCATCAACCGCCATa                                                                                                          >  1:3983512/1‑150 (MQ=255)
                                              cttcttCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGC‑GGGTTAG‑CAGGGTTTTCAACG‑AATT‑CGATAATTTTATCGTTCTCATCAACCGCCATaa                                                                                                         >  1:4201481/1‑150 (MQ=255)
                                                     cagcagTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGC‑GGGTTAA‑CAGGGTTTTCAAAG‑AATT‑CGATAATTTTATTCTTCTCATCAACCGCCATAAACCCaaa                                                                                                  >  2:2471994/1‑150 (MQ=255)
                                                       gcagTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGC‑GGGGTAG‑CAGGTTTTTTCACG‑AATT‑CGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATg                                                                                                >  2:2400761/1‑150 (MQ=255)
                                                        cagTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGC‑GGGTTAG‑CAGGTTTTTCAACG‑AATT‑CGATAATTTTATCGTTCTCATCAACCGCTATAACGCCAAATGc                                                                                               <  2:1512310/150‑1 (MQ=255)
                                                        cagTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGC‑GGGTTAG‑CAGGTTTTTCAACG‑AATT‑CGATAATTTTATCGTTCTCATCAACCGCCCTAACGCCAAATGc                                                                                               >  1:5006443/1‑150 (MQ=255)
                                                        cagTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGC‑GGGTTAG‑CAGGGTTTTCAACG‑AAAT‑CGATAATTTTATCGTTCTCATCAACCCCCATAACGCCAAATGc                                                                                               >  2:281032/1‑150 (MQ=255)
                                                         agTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGC‑GGGTTAG‑CAGGTTTTTCAACG‑AATT‑CGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCg                                                                                              >  1:4613268/1‑150 (MQ=255)
                                                            tCATACAGATAGGCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGC‑GGGTTAG‑CAGGGTTTTCAACG‑AATT‑CGATAATTTTTTCGTTCTCATCAACCGCCATAACGCCAAAAGCGGAg                                                                                           >  2:4999464/1‑150 (MQ=255)
                                                             cATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGC‑GGGTTAG‑CAGGTTTTTCAACG‑AATT‑CGATAATTTTATCGTTCTCATCAACCGCCATAACGCCCAATGCGGAgg                                                                                          >  1:2076367/1‑150 (MQ=255)
                                                             cATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGC‑GGGTTAG‑CAGGATTTTCAACC‑AATT‑CGATAATTTTATTGGTCTTCTCAACCGCCATAACGCCCAAAGCGGAgg                                                                                          >  2:1914949/1‑150 (MQ=255)
                                                              aTACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGC‑GGGCTAG‑CAGGTTTTTCAACG‑AATT‑CGATAATTTTATCGCTCTCATCAACCGCCATAACGCCAAATGCGGAGGc                                                                                         >  2:3515786/1‑150 (MQ=255)
                                                               tACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGC‑GGGTTAG‑CAGGTTTTTCAACG‑AATT‑CGATAATTTTATCGTTCTCATCAACCGCCATAACGCCCAATGCGGAGGct                                                                                        >  1:2561824/1‑150 (MQ=255)
                                                                 cAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGC‑GGGGTAG‑CAGGTTTTTCAACG‑AATT‑CGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGcttc                                                                                      >  1:1657483/1‑150 (MQ=255)
                                                                 cAGATAGGCGGCGAAAAAGACGAAGATCCCCATCCCCGCGAGATTTTTGCTCGGATCGTTGGGCTTGGGCGGG‑GGTTTAG‑CTGTTTTTACAACA‑AATT‑GGATTTTTTTATCTTTCTCACCAACCGCCATAACGCCAAATGCGGAGGcttc                                                                                      <  2:216730/150‑1 (MQ=255)
                                                                  aGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGC‑GGGTTAG‑CAGGTTTTTCAACG‑AATT‑CGATAATTTTATCGTTCTCATCAACCCCCCTAACGCCAAATGCGGAGGcgtct                                                                                     >  2:3295360/1‑150 (MQ=255)
                                                                  aGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGC‑GGGTTAG‑CAGGGTTTTCAACG‑AATT‑CGATAATTTTATTGTTCTCATCAAACGCCATAACGCCAAATGCGGAGGcttct                                                                                     >  2:3461108/1‑150 (MQ=255)
                                                                    aTAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGC‑GGGGTAG‑CAGGGTTTTCAACG‑AATT‑CGGTAATTTTATCGGTCTCATCAACCGCCATAACGCCCAATGCGGAGGCtttttc                                                                                   >  2:197107/1‑150 (MQ=255)
                                                                      aGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGC‑GGGTTAG‑CAGGTTTTTCAACG‑AATT‑CGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCaa                                                                                 >  1:780981/1‑150 (MQ=255)
                                                                              tCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGC‑GGGTTAG‑CAGGTTTTTCAACG‑AATT‑CGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACt                                                                         >  1:3876945/1‑150 (MQ=255)
                                                                                  aGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGC‑GGGGTAG‑CAGGGTTTTCAACG‑AATT‑CGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCa                                                                     >  2:2914123/1‑150 (MQ=255)
                                                                                    aCGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGC‑GGGGTAG‑CAGGTTTTTCAACG‑AATT‑CGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGGACTGGCATa                                                                   >  2:862654/1‑150 (MQ=255)
                                                                                      gTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGC‑GGGTTAG‑CAGGTTTTTCAACG‑AATT‑CGATAATTTTTTTGTTCTCATCAACCGCCATAACGCCCAAATCGGAGGCTTCTTCCATCGGTACTGGCATACa                                                                 >  2:3956073/1‑150 (MQ=255)
                                                                                       tAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGC‑GGGTTAG‑CAGGTTTTTCAACG‑AATT‑CGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACaa                                                                >  1:44926/1‑150 (MQ=255)
                                                                                       tAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGC‑GGGGTAG‑CAGGGTTTTCAACG‑AATT‑CGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACaa                                                                >  1:238376/1‑150 (MQ=255)
                                                                                        aGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGC‑GGGCTTG‑CAGGTTTTTCAACG‑GATT‑CGATAATTTTATCGGTTTCACCAACCCCCACAACGCCAAATGCGGGGGCTTCTTCAATCGGTACTGGCATACAAg                                                               >  2:1683224/1‑150 (MQ=255)
                                                                                          aTACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGC‑GGGGTAG‑CAGGGTTTTCAACG‑AATT‑CGATAATTTTATCGGTCTCATCAACCCCCATAACGCCAAAAGCGGAGGGTTCTTTTATCGGTACTGGCATACAAACa                                                             >  2:399931/1‑150 (MQ=255)
                                                                                           tACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGC‑GGGTTAGCCCGGTTTTTC‑ACG‑AATT‑CGATAATTTTTTCTTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCaa                                                            >  2:3075497/1‑150 (MQ=255)
                                                                                              ccATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGC‑GGGGTAG‑CAGGGTTTTCAACG‑AATT‑CGATAATTTTTTCGTTCTCATCAACCGCCATAACGCTAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACgg                                                         >  2:4615811/1‑150 (MQ=255)
                                                                                                 tACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGC‑GGGTTAG‑CAGGTTTTTCAACG‑AATT‑CGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGc                                                      >  2:1015232/1‑150 (MQ=255)
                                                                                                  aCTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGC‑GGGTTAG‑CAGGTTTTTCAACG‑AATT‑CGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCa                                                     >  1:4902641/1‑150 (MQ=255)
                                                                                                        cAGAGATTTGCTCGGATCGTTCGGCATTGACGGC‑GGGTTAG‑CAGGTTTTTCAACG‑AATT‑CGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTgc                                               >  1:3251352/1‑150 (MQ=255)
                                                                                                         agagATTTGCTCGGATCGTTCGGCATTGACGGC‑GGGTTAG‑CAGGTTTTTCAACG‑AATT‑CGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTgcg                                              >  1:323582/1‑150 (MQ=255)
                                                                                                            gttttGCTCGGCTCGTTGGGCTTGGAGGGC‑GGGTTAG‑CGGTTTTTTCAACG‑ATTT‑CGATATTTTTTTCTTTCCCACCACCCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCt                                           <  2:4482296/148‑1 (MQ=255)
                                                                                                            gaTTTGCTCGGATCGTTCGGCATTGACGGC‑GGGTTAG‑CAGGTTTTTTAACG‑AATT‑CGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCt                                           >  2:602151/1‑150 (MQ=255)
                                                                                                              tttGCTCGGATCGTTCGGCATTGACGGC‑GGGTTAG‑CAGGGTTTTCAACG‑AATT‑CGATAATTTTTTCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCttt                                         >  2:808051/1‑150 (MQ=255)
                                                                                                               ttGCTCGGATCGTTCGGCATTGACGGC‑GGGTTAG‑CAGGGTTTTTAACG‑AATT‑CGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGGACTGGCATACAAGCAACGGTGCAACGTGCGCCtttt                                        >  2:2852058/1‑150 (MQ=255)
                                                                                                               tcccTCGGCTCGTTCGGCTTTGCCGGC‑GGGTTAG‑CAGTTTTTCCAACA‑ATTT‑CTTTATTTTTCTCTTTCTCAACACCCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCtttt                                        <  2:1955466/147‑1 (MQ=255)
                                                                                                                 gCTCGGATCGTTCGGCATTGACGGC‑GGGTTAG‑CAGGTTTTTCAACG‑CATT‑CGATAATTTTATCGTTCTCATCAACCGCCATAAAGCCAAATGCGGAGGCTTGTTTAATCGGTACTGGCATACAAGCAACGGCGAAACGTGCGCCtttttt                                      >  2:1572697/1‑149 (MQ=255)
                                                                                                                  cTCGGATCGTTCGGCATTGACGGC‑GGGTTAG‑CAGGTTTTTCAACG‑AATT‑CGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCg                                     >  1:1964006/1‑150 (MQ=255)
                                                                                                                  cTCGGATCGTTCGGCATTGACGGC‑GGGTTAG‑CAGGGTTTTTAACG‑AATT‑CGATAATTTTATCGTTCTCATCAACCGCCATAACGCCCAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCg                                     >  2:768653/1‑150 (MQ=255)
                                                                                                                       aTCGTTCGGCATTGACGGC‑GGGTTAG‑CAGGTTTTTCAACG‑AATT‑CGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTg                                >  1:4249183/1‑150 (MQ=255)
                                                                                                                         cGTTCGGCATTGACGGC‑GGGTTAG‑CAGGTTTTTCAACG‑AATT‑CGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAACAACGGTGCAACGTGCGCCTTTTTCGACGTGAt                              >  1:567139/1‑150 (MQ=255)
                                                                                                                          tttCGGCATGGGGGGG‑GGTTTAG‑CA‑TTTTTTCCAAG‑AATTCCGATATTTTTATCTTTCTCACCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATc                             <  2:3795546/149‑1 (MQ=255)
                                                                                                                                 aTTGACGGC‑GGGTTAG‑CAGGGTTTTCAACG‑AATT‑CGATAATTTTTTCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAACAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGATAAGc                      >  2:140418/1‑150 (MQ=255)
                                                                                                                                    gACGGC‑GGGTTAG‑CAGGTTTTTCAACG‑AATT‑CGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGATAAGCATa                   >  1:4844113/1‑150 (MQ=255)
                                                                                                                                       ggc‑ggGTTAG‑CAGGTTTTTCAACG‑AATT‑CGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGATAAGCATAcgc                >  2:3059134/1‑150 (MQ=255)
                                                                                                                                       ggc‑ggGTTAG‑CAGGTTTTTCAACG‑AATT‑CGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGATAAGCATAcgc                >  2:1127209/1‑150 (MQ=255)
                                                                                                                                       ggc‑ggGTTAG‑CAGGTTTTTCAACG‑AATT‑CGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGATAAGCATAcgc                >  2:137152/1‑150 (MQ=255)
                                                                                                                                            ggTTAG‑CGGTTTTTACAACA‑AATT‑CGTTATTTTTTTCTTTCCCACCACCCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGATAAGCATACGCGagt            <  2:1861735/150‑1 (MQ=255)
                                                                                                                                            ggTTAG‑CAGGTTTTTCAACG‑AATT‑CGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGATAAGCATACGCGagt            >  2:2567120/1‑150 (MQ=255)
                                                                                                                                             gTTAG‑CAGGTTTTTCAACG‑AATT‑CGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGATAAGCATACGCGagta           >  1:2719109/1‑150 (MQ=255)
                                                                                                                                              ttAG‑CAGGTTTTTCAACG‑AATT‑CGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGATAAGCATACGCGagtag          >  2:3399486/1‑150 (MQ=255)
                                                                                                                                               gtgaggagTTTTTAAAAGAAATT‑TGATATTTTTTTCTTTCTAAACAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGATAAGCATACGCGagta           <  1:1210532/143‑1 (MQ=255)
                                                                                                                                                    aGGTTTTTCAACG‑AATT‑CGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGATAAGCATACGCGAGTAGTcttg     >  2:4256680/1‑150 (MQ=255)
                                                                                                                                                    aGGTTTTTAAACG‑ATTT‑CATTATTTTTATCTTTCCAACCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGATAAGCATACGCGAGTAGTcttg     <  1:4368360/150‑1 (MQ=255)
                                                                                                                                                       tttttCAACG‑AATT‑CGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGATAAGCATACGCGAGTAGTcttgctt  >  2:3927683/1‑150 (MQ=255)
                                                                                                                                                       |                                                                                                                                                       
ATCAAATCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGC‑GGGTTAG‑CAGGTTTTTCAACG‑AATT‑CGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGATAAGCATACGCGAGTAGTCTTGCTT  >  AP012306/3020521‑3020819

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑