Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123063,527,3680AC31.9% 592.8 / 67.2 276L140F (TTA→TTC) rhaSDNA‑binding transcriptional activator, L‑rhamnose‑binding
Reads supporting (aligned to +/- strand):  ref base A (151/37);  new base C (0/88);  total (151/125)
Fisher's exact test for biased strand distribution p-value = 1.32e-42
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 330 total aligned reads displayed.

CGATTTCAGTTTCTCGCCGGGCTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAA‑CCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGATGAGGTGAATTG  >  AP012306/3527219‑3527517
                                                                                                                                                      |                                                                                                                                                     
 gATTTCAGTTTCTCGCCGGGCTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAA‑CCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTAc                                                                                                                                                      >  1:3969002/1‑150 (MQ=255)
  aTTTCAGTTTCTCGCCGGGCTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAA‑CCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTAcc                                                                                                                                                     <  1:2261389/150‑1 (MQ=255)
  aTTTCAGTTTCTCGCCGGGCTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAA‑CCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTAcc                                                                                                                                                     >  1:2087059/1‑150 (MQ=255)
   tttCAGTTTCTCGCCGGGCTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAA‑CCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTAccc                                                                                                                                                    <  2:4823227/150‑1 (MQ=255)
    ttCAGTTTCTCGCCGGGCTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAA‑CCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCt                                                                                                                                                   >  1:1630186/1‑150 (MQ=255)
     tCAGTTTCTCGCCGGGCTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAA‑CCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTc                                                                                                                                                  <  1:1017343/150‑1 (MQ=255)
      cAGTTTCTCGCCGGGCTGAATCAGTTGCTGCCTCAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAA‑CCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCg                                                                                                                                                 >  2:1569340/1‑150 (MQ=255)
        gTTTCTCGCCGGGCTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAA‑CCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGAc                                                                                                                                               <  1:3418892/150‑1 (MQ=255)
         tttCTCGCCGGGCTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAA‑CCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGAcc                                                                                                                                              >  2:4371469/1‑150 (MQ=255)
         tttCTCGCCGGGCTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAA‑CCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGAcc                                                                                                                                              >  1:1201017/1‑150 (MQ=255)
             tcGCCGGGCTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAA‑CCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCa                                                                                                                                          <  2:4221966/150‑1 (MQ=255)
                ccGGGCTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAA‑CCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTc                                                                                                                                       >  2:936738/1‑150 (MQ=255)
                 ggggTTGAATCGGTTGCTGCCACAAGAGCTGGAGGGGCAGTATCCGTCTCACTGGCGCGTTAC‑CCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTCCACAGATGGAACAGCAGGAAGGGAAAAATGATTTCCCCTCGACCGCCAGTcg                                                                                                                                      <  2:4917215/149‑1 (MQ=255)
                  gggCTGAATCAGTTGCTGCGACAAGAGCTGGATGGGCAGTATGGGTCTCAATGGCGGGTTAA‑CCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTcgc                                                                                                                                     >  2:659562/1‑150 (MQ=255)
                     cTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAA‑CCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCgag                                                                                                                                  >  2:2576327/1‑150 (MQ=255)
                      tGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAA‑CCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTCCCCTCGACCGCCAGTCGCgaga                                                                                                                                 <  1:330880/150‑1 (MQ=255)
                        aaTCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAA‑CCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTCCCCTCGACCGCCAGTCGCGAGATc                                                                                                                               <  2:4030735/150‑1 (MQ=255)
                         aTCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAA‑CCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTCCCCTCGACCGCCAGTCGCGAGATCt                                                                                                                              <  1:3100186/150‑1 (MQ=255)
                          tCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAA‑CCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCtt                                                                                                                             >  1:444158/1‑150 (MQ=255)
                            aGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAA‑CCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGt                                                                                                                           >  1:450590/1‑150 (MQ=255)
                                cctgcCAAAAGAGCGGGAGGGGCAGTATCCGTCTCACGGGCGCGTTAA‑CCACAGCGTATTGCAGCAGGGGCGACAGCGGGTTGCACAGATGGAACAGCAGGAAGGGAAAAATGTTTTCCCCCCGACCGCCAGTCGCGAGATCTTGTTTAt                                                                                                                       <  2:954521/149‑1 (MQ=255)
                                  ggcCACAAGAGCGGGATGGGCAGTACCCGTCTCACGGGCGCGTTAA‑CCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACGGAGGGAACAGCAGGAGGGGAAAAATGATTTCCCCCCGACCGCCAGTCGCGAGATCTTGTTTATGc                                                                                                                     <  2:1716560/149‑1 (MQ=255)
                                      acaAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAA‑CCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAAtt                                                                                                                 >  2:2046918/1‑150 (MQ=255)
                                      acaAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAA‑CCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAAtt                                                                                                                 >  1:1144257/1‑150 (MQ=255)
                                       caAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAA‑CCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTa                                                                                                                >  2:787808/1‑150 (MQ=255)
                                              tGGATGGGCAGTCTCCGTCTCACTGGCGCGTTAA‑CCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAGGGGAAAAATGTTTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGctct                                                                                                         <  2:3194434/150‑1 (MQ=255)
                                              tGGATGGGCAGTATCCGTCTCACTGGCGCGTTAA‑CCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGctct                                                                                                         >  2:3410311/1‑150 (MQ=255)
                                                gATGGGCAGTATCCGTCTCACTGGCGCGTTAA‑CCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTtg                                                                                                       <  1:4626351/150‑1 (MQ=255)
                                                   ggggAGTATCCGTCTCCCTGGCGCTTTAA‑CCACGGCGTATGGCAGGAGGTGCGACGGCTGTTTGCACAGAGGGAACAGAAGGAAGGGAAAAATGATTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTtgctg                                                                                                    <  2:441549/150‑1 (MQ=255)
                                                    ggCAGTACCCGTCTCACTGGCGCGTTAC‑CCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGAAAAATGATTTCCCCCCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTtgctgc                                                                                                   <  2:3899722/150‑1 (MQ=255)
                                                      gaGTATCCGTAACACTGGCGTGTTAC‑CCACAGCGTATGGCAGGAGGTGCGACAGGTGGTAGCACAAATGGAACAGCAGGAAGGGGAAAATGTTTTCCCCCCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGt                                                                                                 <  2:232156/149‑1 (MQ=255)
                                                      cAGTATCCGTCTCACTGGCGCGTTAA‑CCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGt                                                                                                 >  1:876089/1‑150 (MQ=255)
                                                          ttCCGTCTCACTGGCGCTTTAA‑CCACAGCGTATGGCAGCAGGGGCGACAGGTGGTTGCAAAGAGGGAACAGCAGGAAGGGGAAAATTATTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTaaaa                                                                                             <  2:3769754/149‑1 (MQ=255)
                                                          aTCCGTCTCACTGGCGCGTTAA‑CCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTaaaa                                                                                             >  1:2925139/1‑150 (MQ=255)
                                                           tCCGTCTCACTGGCGCGTTAA‑CCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAg                                                                                            <  1:4233712/150‑1 (MQ=255)
                                                           tCCGTCTCACTGGCGCGTAAA‑CCACAGCGTATTGCAGGAGGTGCGACAGCTGGTTGCACAGATGAAACAGGAGGAGGGGGAAAATGATTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAg                                                                                            <  2:1784892/150‑1 (MQ=255)
                                                           cccGTCCCACTGGCGCGTTAA‑CCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGAAAAATGATTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAg                                                                                            <  2:310689/149‑1 (MQ=255)
                                                             cGTCTCGCGGGCGCGTTAA‑CCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCa                                                                                          <  1:4359815/150‑1 (MQ=255)
                                                             cGTCTCACTGGCGCGTTAA‑CCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCa                                                                                          >  1:3898698/1‑150 (MQ=255)
                                                              gTCTCACTGGCGCGTTAA‑CCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAg                                                                                         <  1:4930961/150‑1 (MQ=255)
                                                              gTCTCACTGGCGCGTTAA‑CCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAAATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAg                                                                                         >  2:1602052/1‑150 (MQ=255)
                                                                ctcACTGGCGCGTTAA‑ACACAGTGTATTGCAGCAGGTGGGACAGCAGGTCGCCCGGAGGGACAAGGAGGAGGGGGAAAATGATTCACCCTTGCCCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGtt                                                                                       <  2:141355/150‑1 (MQ=255)
                                                                   acgggCGCTTTAC‑CCACAGCGTATTGCAGCAGGTGCGACAGCTGGTGCCACAGAGGGAACAGCAGGAAGGGGAAAATGATTTCCCCCCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGc                                                                                    <  2:4712838/147‑1 (MQ=255)
                                                                    cgggCGCGTTAA‑CCACAGCGTATTGCAGCAGGTGCGCCAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCa                                                                                   <  2:4078639/148‑1 (MQ=255)
                                                                     tGGCGCGTTAA‑CCACAGCTTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAg                                                                                  <  2:543806/150‑1 (MQ=255)
                                                                     tGGCGCGTTAA‑CCACAGCGTATTGCAGCGGGTGCGACAGCTGGTTGCACAGGTGGAACAGCAGGAAGGGGAAAATGATTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAg                                                                                  <  1:526287/150‑1 (MQ=255)
                                                                     tGGCGCGTTAA‑CCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAg                                                                                  <  1:603430/150‑1 (MQ=255)
                                                                     cgggcggTTAACCCACGGGGTTTTGCAGCGGGTGCGACAGCTGGTTGCACAGATGGAACAGAAGGAGGGGAAAAATGATTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCa                                                                                   <  2:2491750/144‑1 (MQ=255)
                                                                       gcgcGTTAA‑CCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGga                                                                                >  1:3566590/1‑150 (MQ=255)
                                                                       gcgcGTAAC‑CCACAGCGTATGGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGga                                                                                <  1:368192/150‑1 (MQ=255)
                                                                        cgtgTTAA‑CCAAAGCGTATTGCAGTAGGGGCAACAGCTGGTTGCACAGAAGGAACAAGAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGgag                                                                               <  2:1060270/147‑1 (MQ=255)
                                                                         gcgTTAA‑CCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGgaga                                                                              <  1:2547587/150‑1 (MQ=255)
                                                                          cgTTAA‑CCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGaa                                                                             <  1:3029147/150‑1 (MQ=255)
                                                                          cgTTAA‑CCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGaa                                                                             >  1:4051046/1‑150 (MQ=255)
                                                                              aa‑CCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTg                                                                         >  2:327215/1‑150 (MQ=255)
                                                                              aa‑CCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTg                                                                         >  1:2484460/1‑150 (MQ=255)
                                                                               a‑CCACAGCGTTTGGCAGGAGGTGCGACAGCTGGTTGCACAGATGAAAAAGCAGGAGGGGAAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTgg                                                                        <  2:36943/150‑1 (MQ=255)
                                                                                 ccACAGCGTATGGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGAAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGa                                                                       <  2:877590/150‑1 (MQ=255)
                                                                                     aGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAAc                                                                   >  2:1288687/1‑150 (MQ=255)
                                                                                      gCGTATTGCAGCAGGTGCGACAGCTGGTTGAACAGATGGAACAGCAGGAAGGGAAAAATGTTTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACa                                                                  <  2:1117021/150‑1 (MQ=255)
                                                                                           ttGCAGCAGGCGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCa                                                             >  2:3317607/1‑150 (MQ=255)
                                                                                            tGCAGCAGGTGCAACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCAt                                                            <  1:177794/150‑1 (MQ=255)
                                                                                              cagcagGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCa                                                          >  1:1415736/1‑150 (MQ=255)
                                                                                                gcagGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACg                                                        >  1:2186862/1‑150 (MQ=255)
                                                                                                 cagGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGt                                                       <  1:4292912/150‑1 (MQ=255)
                                                                                                      gCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCaa                                                  >  2:4112628/1‑150 (MQ=255)
                                                                                                        gACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACt                                                <  1:3242976/150‑1 (MQ=255)
                                                                                                         aCAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACtt                                               <  1:2775399/150‑1 (MQ=255)
                                                                                                         aCAGCGGGTTGCAGAGAGGGAACGGCAGGAGGGGGAAAATGATTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACtt                                               <  1:2851114/150‑1 (MQ=255)
                                                                                                          cAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTg                                              >  2:252380/1‑150 (MQ=255)
                                                                                                             cTGGTTGCACAGATGGAACAGCAGGAAGGGAAAAATGATTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCtt                                           <  2:4287071/150‑1 (MQ=255)
                                                                                                             cTGGTGGCACAGAGGAAACAGAAGAAAGGGGAAAATGTTTCCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCtt                                           <  2:2871151/150‑1 (MQ=255)
                                                                                                              tGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTc                                          <  1:4872591/150‑1 (MQ=255)
                                                                                                              tGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGGGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTc                                          >  2:3716318/1‑150 (MQ=255)
                                                                                                                gTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTctg                                        >  2:2096818/1‑150 (MQ=255)
                                                                                                                 ttGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTctgg                                       >  1:1311986/1‑150 (MQ=255)
                                                                                                                    cacaGAGGGAACAGCAGGAAGGGAAAATTGTTTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTctggcct                                    <  2:2752115/150‑1 (MQ=255)
                                                                                                                      caGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTctggcctgg                                  >  1:3607974/1‑150 (MQ=255)
                                                                                                                       aGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTctggcctggc                                 >  1:4735332/1‑150 (MQ=255)
                                                                                                                           ggACCAGCAGGAAGGGGAAAATGTTCTTCCCCCTGCCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGATCCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTgga                             <  2:3501170/150‑1 (MQ=255)
                                                                                                                           ggAACAGCGGGAAGGGGAAAATGATTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTgga                             <  1:3493442/150‑1 (MQ=255)
                                                                                                                            gAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTggag                            >  2:4957043/1‑150 (MQ=255)
                                                                                                                              aCAGCAGGAAGGGGAAAATGATTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTggagga                          <  2:4159030/150‑1 (MQ=255)
                                                                                                                                agcagGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGAcc                        >  2:4571520/1‑150 (MQ=255)
                                                                                                                                  cagGGAGTGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGCACTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCTTCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCAt                      >  2:2543558/1‑150 (MQ=255)
                                                                                                                                  cagGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCAt                      >  1:2583484/1‑150 (MQ=255)
                                                                                                                                   agGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCAtt                     >  2:3963241/1‑150 (MQ=255)
                                                                                                                                      aaGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTg                  >  1:4131820/1‑150 (MQ=255)
                                                                                                                                        ggggAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGcc                >  1:3162372/1‑150 (MQ=255)
                                                                                                                                         gggAAAATGATTTGCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCg               <  1:1677778/150‑1 (MQ=255)
                                                                                                                                          ggAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGa              >  2:2969543/1‑150 (MQ=255)
                                                                                                                                           gAAAATGTTTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGAt             <  2:2687380/150‑1 (MQ=255)
                                                                                                                                           gAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGAt             >  2:1844825/1‑150 (MQ=255)
                                                                                                                                             aaaTGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGATGa           <  1:199731/150‑1 (MQ=255)
                                                                                                                                                 gttttCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGATGAGGTg       <  2:4462087/148‑1 (MQ=255)
                                                                                                                                                   tttCCCCCCGTCCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGATGAGGTGaa     <  1:2407763/150‑1 (MQ=255)
                                                                                                                                                   tttACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGATGAGGTGaa     >  2:1216238/1‑150 (MQ=255)
                                                                                                                                                    ttACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGATGAGGTGAAt    >  2:3133230/1‑150 (MQ=255)
                                                                                                                                                      aCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGATGAGGTGAATTg  >  1:70864/1‑150 (MQ=255)
                                                                                                                                                      aCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGATGAGGTGAATTg  >  1:1255637/1‑150 (MQ=255)
                                                                                                                                                      |                                                                                                                                                     
CGATTTCAGTTTCTCGCCGGGCTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAA‑CCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGATGAGGTGAATTG  >  AP012306/3527219‑3527517

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑