BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AP012306	1,486,241	G→A	intergenic (‑2088/‑492)	thrS ← / → yniD	threonyl‑tRNA synthetase/predicted protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AP012306	1,486,241	0	G	A	100.0%	1622.4 / NA	407	intergenic (‑2088/‑492)	thrS/yniD	threonyl‑tRNA synthetase/predicted protein
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (206/201); total (206/201)

Only 100 of 416 total aligned reads displayed.

CCAATAGCACAAGAGCTTTTAAACAAAAAACTGGTGATGAGTATTTTAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCGCCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAGCTACAGCACAGGGAACCCCTGCATTATACATCGCCATGAGCAAGGGTAATGAAGACGTCGTGTTATCTTATATATCGACGCTGGGTGCTTTTGCA  >  AP012306/1486094‑1486387

ccAATAGCACAAGAGCTTTTAAACAAAAAACTGGTGATGAGTATTTTAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCAcc                                                                                                                                                  <  1:1966955/150‑1 (MQ=255)

 cAATAGCACAAGAGCTTTTAAACAAAAAACTGGTGATGAGTATTTTAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCt                                                                                                                                                 >  1:554498/1‑150 (MQ=255)

  aaTAGCACAAGAGCTTTTAAACAAAAAACTGGTGATGAGTATTTTAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCtt                                                                                                                                                >  1:297257/1‑150 (MQ=255)

   aTAGCACAAGAGCTTTTAAACAAAAAACTGGTGATGAGTATTTTAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCttt                                                                                                                                               <  1:4348872/150‑1 (MQ=255)

    tAGCACAAGAGCTTTTAAACAAAAAACTGGTGATGAGTATTTTAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTa                                                                                                                                              >  2:3041164/1‑150 (MQ=255)

     aGCACAAGAGCTTTTAAACAAAAAACTGGTGATGAGTATTTTAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTaa                                                                                                                                             >  1:1103707/1‑150 (MQ=255)

      gCACAAGAGCTTTTAAACAAAAAACTGGTGATGAGTATTTTAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTaaa                                                                                                                                            <  2:2938319/150‑1 (MQ=255)

        acaAGAGCTTTTAAACAAAAAACTGGTGATGAGCATTTTAGCCATGAAAATACATGATGGAGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATa                                                                                                                                          >  1:1379164/1‑150 (MQ=255)

           agagCTTTTAAACAAAAAACTGGTGATGAGTATTTTAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACaa                                                                                                                                       <  2:135974/150‑1 (MQ=255)

             agCTTTTAAACAAAAAACTGGTGATGAGTATTTTAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGt                                                                                                                                     >  1:2639902/1‑150 (MQ=255)

               cTTTTAAACAAAAAACTGGTGATGAGTATTTTAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTg                                                                                                                                   >  1:1527373/1‑150 (MQ=255)

                 tttAAACAAAAAACTGGTGATGAGTATTTTAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAg                                                                                                                                 <  2:472028/150‑1 (MQ=255)

                 tttAAACAAAAAACTGGTGATGAGTATTTTAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAg                                                                                                                                 >  1:2444799/1‑150 (MQ=255)

                      aCAAAAAACTGGTGATGAGTATTTTAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAg                                                                                                                            >  2:4974805/1‑150 (MQ=255)

                       cAAAAAACTGGTGATGAGTATTTTAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGc                                                                                                                           >  2:3454593/1‑150 (MQ=255)

                         aaaaaCTGGTGATGAGTATTTTAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTa                                                                                                                         >  2:872787/1‑150 (MQ=255)

                            aaCTGGTGATGAGTATTTTAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAGATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAAca                                                                                                                      <  1:963680/150‑1 (MQ=255)

                            aaCTGGTGATGAGTATTTTAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAAca                                                                                                                      >  1:3280705/1‑150 (MQ=255)

                            aaCTGGTGATGAGTATTTTAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAAca                                                                                                                      >  2:4851929/1‑150 (MQ=255)

                             aCTGGTGATGAGTATTTTAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAAcat                                                                                                                     <  1:2820087/150‑1 (MQ=255)

                              cTGGTGATGAGTATTTTAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAAcatc                                                                                                                    >  2:2421712/1‑150 (MQ=255)

                              cTGGTGATGAGTATTTTAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAAcatc                                                                                                                    >  1:3628520/1‑150 (MQ=255)

                                ggTGATGAGTATTTTAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAAcatcat                                                                                                                  <  2:2560009/150‑1 (MQ=255)

                                ggTGATGAGTATTTTAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAAcatcat                                                                                                                  >  2:2305679/1‑150 (MQ=255)

                                  tgatgaGTATTTTAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAACATCATgg                                                                                                                <  2:1278108/150‑1 (MQ=255)

                                    attaGTATTTTAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGAt                                                                                                              <  2:2361472/147‑1 (MQ=255)

                                        gTATTTTAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAACATCATg                                                                                                                 <  1:1211114/143‑1 (MQ=255)

                                            tttAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTaaa                                                                                                      >  1:1861763/1‑150 (MQ=255)

                                            tttAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTaaa                                                                                                      <  2:4439685/150‑1 (MQ=255)

                                                gCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGa                                                                                                  <  1:2165985/150‑1 (MQ=255)

                                                 ccATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAg                                                                                                 >  1:778537/1‑150 (MQ=255)

                                                  cATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAGc                                                                                                >  1:114115/1‑150 (MQ=255)

                                                  cATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAGc                                                                                                >  1:4035755/1‑150 (MQ=255)

                                                   aTGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAGCt                                                                                               >  1:4433762/1‑150 (MQ=255)

                                                   aTGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAGCt                                                                                               >  1:3429485/1‑150 (MQ=255)

                                                     gAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAGCTAc                                                                                             >  2:3721389/1‑150 (MQ=255)

                                                       aaaTACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAGCTACAg                                                                                           <  2:3288319/150‑1 (MQ=255)

                                                       aaaTACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAGCTACAg                                                                                           >  2:2140253/1‑150 (MQ=255)

                                                       aaaTACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAGCTACAg                                                                                           <  1:3067543/150‑1 (MQ=255)

                                                          tACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAGCTACAGcac                                                                                        >  1:1058632/1‑150 (MQ=255)

                                                          tACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAGCTACAGcac                                                                                        <  2:2892765/150‑1 (MQ=255)

                                                            cATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAGCTACAGCACAg                                                                                      >  2:1911697/1‑150 (MQ=255)

                                                            cATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAGCTACAGCACAg                                                                                      <  2:2731478/150‑1 (MQ=255)

                                                             atgatgGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAGCTACAGCACAgg                                                                                     >  2:3819831/1‑150 (MQ=255)

                                                              tgatgGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAGCTACAGCACAggg                                                                                    >  1:657604/1‑150 (MQ=255)

                                                              tgatgGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAGCTACAGCACAggg                                                                                    <  1:4810594/150‑1 (MQ=255)

                                                              tgatgGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAGCTACAGCACAggg                                                                                    >  2:3640093/1‑150 (MQ=255)

                                                               gatgGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAGCTACAGCACAGGGa                                                                                   <  2:553409/150‑1 (MQ=255)

                                                                atgGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAGCTACAGCACAGGGaa                                                                                  >  2:188254/1‑150 (MQ=255)

                                                                 tgGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAGCTACAGCACAGGGAAc                                                                                 >  1:3918100/1‑150 (MQ=255)

                                                                  ggTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAGCTACAGCACAGGGAAcc                                                                                <  1:4219454/150‑1 (MQ=255)

                                                                      aGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAGCTACAGCACAGGGAACCCCTg                                                                            <  2:1222662/150‑1 (MQ=255)

                                                                         cAGGATTATACGCCGCAATGGAAAATAATCAGCCTTTGTGTGTCACACGGTTCCTCTCTAAAGTTAATGGCATCACCTTTAAATACAAGTTGAGCAAATCTAACATCATGGATTTATTAAAAGGAGCTACAGCAAAGTGAACCCCTGCAt                                                                         >  2:639200/1‑150 (MQ=255)

                                                                          aGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAGCTACAGCACAGGGAACCCCTGCAtt                                                                        >  2:4195083/1‑150 (MQ=255)

                                                                          aGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAGCTACAGCACAGGGAACCCCTGCAtt                                                                        >  2:1625468/1‑150 (MQ=255)

                                                                          aGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAGCTACAGCACAGGGAACCCCTGCAtt                                                                        >  1:2083420/1‑150 (MQ=255)

                                                                           ggATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAGCTACAGCACAGGGAACCCCTGCATta                                                                       >  1:2549310/1‑150 (MQ=255)

                                                                             aTTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAGCTACAGCACAGGGAACCCCTGCATtata                                                                     <  1:3849041/150‑1 (MQ=255)

                                                                             aTTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAGCTACAGCACAGGGAACCCCTGCATtata                                                                     <  2:1025584/150‑1 (MQ=255)

                                                                               tataCGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAGCTACAGCACAGGGAACCCCTGCATTATACa                                                                   <  1:4956107/150‑1 (MQ=255)

                                                                                 taCGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAGCTACAGCACAGGGAACCCCTGCATTATACATc                                                                 <  2:3131756/150‑1 (MQ=255)

                                                                                 taCGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAGCTACAGCACAGGGAACCCCTGCATTATACATc                                                                 >  1:610714/1‑150 (MQ=255)

                                                                                  aCGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAGCTACAGCACAGGGAACCCCTGCATTATACATCg                                                                >  1:3132399/1‑150 (MQ=255)

                                                                                         aaTGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAGCTACAGCACAGGGAACCCCTGCATTATACATCGCCATGAg                                                         <  2:1422818/150‑1 (MQ=255)

                                                                                            ggAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAGCTACAGCACAGGGAACCCCTGCATTATACATCGCCATGAGCaa                                                      >  1:410974/1‑150 (MQ=255)

                                                                                            ggAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAGCTACAGCACAGGGAACCCCTGCATTATACATCGCCATGAGCaa                                                      >  1:3361305/1‑150 (MQ=255)

                                                                                             gAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAGCTACAGCACAGGGAACCCCTGCATTATACATCGCCATGAGCAAg                                                     <  2:4292626/150‑1 (MQ=255)

                                                                                              aaaaTAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAGCTACAGCACAGGGAACCCCTGCATTATACATCGCCATGAGCAAgg                                                    >  2:1380141/1‑150 (MQ=255)

                                                                                               aaaTAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAGCTACAGCACAGGGAACCCCTGCATTATACATCGCCATGAGCAAggg                                                   >  1:925931/1‑150 (MQ=255)

                                                                                                aataatCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAGCTACAGCACAGGGAACCCCTGCATTATACATCGCCATGAGCAAGGGt                                                  <  1:3796643/150‑1 (MQ=255)

                                                                                                aataatCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAGCTACAGCACAGGGAACCCCTGCATTATACATCGCCATGAGCAAGGGt                                                  >  1:4751287/1‑150 (MQ=255)

                                                                                                  taatCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAGCTACAGCACAGGGAACCCCTGCATTATACATCGCCATGAGCAAGGGTaa                                                >  1:1723452/1‑150 (MQ=255)

                                                                                                   aatCATCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAGCTACAGCACAGGGAACCCCTGCATTATACATCGCCATGAGCAAGGGTAAt                                               <  2:1770432/150‑1 (MQ=255)

                                                                                                   aatCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAGCTACAGCACAGGGAACCCCTGCATTATACATCGCCATGAGCAAGGGTAAt                                               <  2:4598578/150‑1 (MQ=255)

                                                                                                    atCACCCCTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAGCTACAGCACAGGGAACCCCTGCATTATACATCGCCATGAGCAAGGGTAATg                                              <  2:3515590/150‑1 (MQ=255)

                                                                                                       aCCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAGCTACAGCACAGGGAACCCCTGCATTATACATCGCCATGAGCAAGGGTAATGAAg                                           >  2:4121223/1‑150 (MQ=255)

                                                                                                       aCCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAGCTACAGCACAGGGAACCCCTGCATTATACATCGCCATGAGCAAGGGTAATGAAg                                           >  1:491362/1‑150 (MQ=255)

                                                                                                        cccTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAGCTACAGCACAGGGAACCCCTGCATTATACATCGCCATGAGCAAGGGTAATGAAGa                                          <  2:2209555/150‑1 (MQ=255)

                                                                                                               tgtgtCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAATTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAGCTACAGCACAGGGAACCCCTGCATTATACATCGCCATGAGCAAGGGTAATGAAGACGTCgtg                                   >  1:2350971/1‑150 (MQ=255)

                                                                                                               tgtgtCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAGCTACAGCACAGGGAACCCCTGCATTATACATCGCCATGAGCAAGGGTAATGAAGACGTCgtg                                   <  2:1517462/150‑1 (MQ=255)

                                                                                                                gtgtCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAGCTACAGCACAGGGAACCCCTGCATTATACATCGTCATGAGCAAGGGTAATGAAGACGTCgtgt                                  >  1:3573391/1‑150 (MQ=255)

                                                                                                                gtgtCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAGCTACAGCACAGGGAACCCCTGCATTATACATCGCCATGAGCAAGGGTAATGAAGACGTCgtgt                                  <  2:1994135/150‑1 (MQ=255)

                                                                                                                 tgtCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAGCTACAGCACAGGGAACCCCTGCATTATACATCGCCATGAGCAAGGGTAATGAAGACGTCGTGtt                                 <  2:2674597/150‑1 (MQ=255)

                                                                                                                 tgtCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAGCTACAGCACAGGGAACCCCTGCATTATACATCGCCATGAGCAAGGGTAATGAAGACGTCGTGtt                                 >  2:5008481/1‑150 (MQ=255)

                                                                                                                 tgtCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAGCTACAGCACAGGGAACCCCTGCATTATACATCGCCATGAGCAAGGGTAATGAAGACGTCGTGtt                                 <  2:1125127/150‑1 (MQ=255)

                                                                                                                   tCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAGCTACAGCACAGGGAACCCCTGCATTATACATCGCCATGAGCAAGGGTAATGAAGACGTCGTGTTAt                               >  2:3362984/1‑150 (MQ=255)

                                                                                                                     acacGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAGCTACAGCACAGGGAACCCCTGCATTATACATCGCCATGAGCAAGGGTAATGAAGACGTCGTGTTATCt                             <  2:1205010/150‑1 (MQ=255)

                                                                                                                      cacGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAGCTACAGCACAGGGAACCCCTGCATTATACATCGCCATGAGCAAGGGTAATGAAGACGTCGTGTTATCtt                            <  2:74218/150‑1 (MQ=255)

                                                                                                                         ggTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAGCTACAGCACAGGGAACCCCTGCATTATACATCGCCATGAGCAAGGGTAATGAAGACGTCGTGTTATCTtata                         <  1:806316/150‑1 (MQ=255)

                                                                                                                          gTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAGCTACAGCACAGGGAACCCCTGCATTATACATCGCCATGAGCAAGGGTAATGAAGACGTCGTGTTATCTtatat                        <  1:16610/150‑1 (MQ=255)

                                                                                                                            tCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAGCTACAGCACAGGGAACCCCTGCATTATACATCGCCATGAGCAAGGGTAATGAAGACGTCGTGTTATCTTatatat                      <  1:4513754/150‑1 (MQ=255)

                                                                                                                               tctctAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAGCTACAGCACAGGGAACCCCTGCATTATACATCGCCATGAGCAAGGGTAATGAAGACGTCGTGTTATCTTATATATCGa                   >  1:4604642/1‑150 (MQ=255)

                                                                                                                                ctctAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAGCTACAGCACAGGGAACCCCTGCATTATACATCGCCATGAGCAAGGGTAATGAAGACGTCGTGTTATCTTATATATCGAc                  <  2:4659438/150‑1 (MQ=255)

                                                                                                                                 tctAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAGCTACAGCACAGGGAACCCCTGCATTATACATCGCCATGAGCAAGGGTAATGAAGACGTCGTGTTATCTTATATATCGACg                 <  2:4050257/150‑1 (MQ=255)

                                                                                                                                      aaTTAATGGCATCACCTTTAAATACAATTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAGCTACAGCACAGGGAACCCCTGCATTATGCATCGCCATGAGGAAGGGTAATGAAGACGTCGTGTTATCTTATATATCGACGCTggg            <  2:4557684/150‑1 (MQ=255)

                                                                                                                                       aTTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAGCTACAGCACAGGGAACCCCTGCATTATACATCGCCATGAGCAAGGGTAATGAAGACGTCGTGTTATCTTATATATCGACGCTGGGt           <  1:3988386/150‑1 (MQ=255)

                                                                                                                                       aTTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAGCTACAGCACAGGGAACCCCTGCATTATACATCGCCATGAGCAAGGGTAATGAAGACGTCGTGTTATCTTATATATCGACGCTGGGt           >  1:1307044/1‑150 (MQ=255)

                                                                                                                                         tAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAGCTACAGCACAGGGAACCCCTGCATTATACATCGCCATGAGCAAGGGTAATGAAGACGTCGTGTTATCTTATATATCGACGCTGGGTGc         >  2:1837890/1‑150 (MQ=255)

                                                                                                                                          aaTGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAGCTACAGCACAGGGAACCCCTGCATTATACATCGCCATGAGCAAGGGTAATGAAGACGTCGTGTTATCTTATATATCGACGCTGGGTGCt        <  2:4015619/150‑1 (MQ=255)

                                                                                                                                           aTGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAGCTACAGCACAGGGAACCCCTGCATTATACATCGCCATGAGCAAGGGTAATGAAGACGTCGTGTTATCTTATATATCGACGCTGGGTGCtt       >  1:2495361/1‑150 (MQ=255)

CCAATAGCACAAGAGCTTTTAAACAAAAAACTGGTGATGAGTATTTTAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCGCCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAGCTACAGCACAGGGAACCCCTGCATTATACATCGCCATGAGCAAGGGTAATGAAGACGTCGTGTTATCTTATATATCGACGCTGGGTGCTTTTGCA  >  AP012306/1486094‑1486387

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`