BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AP012306	3,407,932	C→T	S40L (TCA→TTA)	wzyE →	predicted Wzy protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AP012306	3,407,932	0	C	T	100.0%	1606.0 / NA	393	S40L (TCA→TTA)	wzyE	predicted Wzy protein
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (186/207); total (186/207)

Only 100 of 398 total aligned reads displayed.

GCGTTGGCGATTGCCGCCAGGGAGGTCGCATGAGTCTGCTGCAATTCAGTGGCCTGTTTGTTGTCTGGCTGCTCTGCACGCTGTTTATTGCCACGCTGACCTGGTTTGAGTTTCGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTCATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCGCTGACCAGCGTGCTGGTATTTCGCTTTGATGTTGGTGTCGCGCCGCCAGAAATCTTGTTGCAGGCGTTGCTTTCTGCGGGCTGCTTCTACGCGGTTTACTATGTCACCT  >  AP012306/3407785‑3408078

gCGTTGGCGATTGCCGCCAGGGAGGTCGCATGAGTCTGCTGCAATTCAGTGGCCTGTTTGTTGTCTGGCTGCTCTGCACGCTGTTTATTGCCACGCTGACCTGGTTTGAGTTTCGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTTAt                                                                                                                                                  <  2:732756/150‑1 (MQ=255)

  gTTGGCGATTGCCGCCAGGGAGGTCGCATGAGTCTGCTGCAATTCAGTGGCCTGTTTGTTGTCTGGCTGCTCTGCACGCTGTTTATTGCCACGCTGACCTGGTTTGAGTTTCGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTTATTg                                                                                                                                                >  2:4572800/1‑150 (MQ=255)

     ggCGATTGCCGCCAGGGAGGTCGCATGAGTCTGCTGCAATTCAGTGGCCTGTTTGTTGTCTGGCTGCTCTGCACGCTGTTTATTGCCACGCTGACCTGGTTTGAGTTTCGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTTATTGCTg                                                                                                                                             >  2:3994170/1‑150 (MQ=255)

       cGATTGCCGCCAGGGAGGTCGCATGAGTCTGCTGCAATTCAGTGGCCTGTTTGTTGTCTGGCTGCTCTGCACGCTGTTTATTGCCACGCTGACCTGGTTTGAGTTTCGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGtt                                                                                                                                           <  1:181695/150‑1 (MQ=255)

         aTTGCCGCCAGGGAGGTCGCATGAGTCTGCTGCAATTCAGTGGCCTGTTTGTTGTCTGGCTGCTCTGCACGCTGTTTATTGCCACGCTGACCTGGTTTGAGTTTCGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGtttt                                                                                                                                         <  2:1738571/150‑1 (MQ=255)

         aTTGCCGCCAGGGAGGTCGCATGAGTCTGCTGCAATTCAGTGGCCTGTTTGTTGTCTGGCTGCTCTGCACGCTGTTTATTGCCACGCTGACCTGGTTTGAGTTTCGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGtttt                                                                                                                                         <  1:130307/150‑1 (MQ=255)

          tcgccgccAGGGAGGTCGCATGAGTCTGCTGCAATTCAGTGGCCTGTTTGTTGTCTGGCTGCTCTGCACGCTGTTTATTGCCACGCTGACCTGGTTTGAGTTTCGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGttttt                                                                                                                                        <  1:1108015/148‑1 (MQ=255)

               gccAGGGAGGTCGCATGAGTCTGCTGCAATTCAGTGGCCTGTTTGTTGTCTGGCTGCTCTGCACGCTGTTTATTGCCACGCTGACCTGGTTTGAGTTTCGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCa                                                                                                                                   <  2:4225302/150‑1 (MQ=255)

               gccAGGGAGGTCGCATGAGTCTGCTGCAATTCAGTGGCCTGTTTGTTGTCTGGCTGCTCTGCACGCTGTTTATTGCCACGCTGACCTGGTTTGAGTTTCGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCa                                                                                                                                   <  1:2924389/150‑1 (MQ=255)

                ccAGGGAGGTCGCATGAGTCTGCTGCAATTCAGTGGCCTGTTTGTTGTCTGGCTGCTCTGCACGCTGTTTATTGCCACGCTGACCTGGTTTGAGTTTCGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCAc                                                                                                                                  >  2:3760630/1‑150 (MQ=255)

                   gggAGGTCGCATGAGTCTGCTGCAATTCAGTGGCCTGTTTGTTGTCTGGCTGCTCTGCACGCTGTTTATTGCCACGCTGACCTGGTTTGAGTTTCGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCtt                                                                                                                               <  2:1792825/150‑1 (MQ=255)

                   gggAGGTCGCATGAGTCTGCTGCAATTCAGTGGCCTGTTTGTTGTCTGGCTGCTCTGCACGCTGTTTATTGCCACGCTGACCTGGTTTGAGTTTCGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCtt                                                                                                                               <  1:3003036/150‑1 (MQ=255)

                   gggAGGTCGCATGAGTCTGCTGCAATTCAGTGGCCTGTTTGTTGTCTGGCTGCTCTGCACGCTGTTTATTGCCACGCTGACCTGGTTTGAGTTTCGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCtt                                                                                                                               <  2:3099892/150‑1 (MQ=255)

                   gggAGGTCGCATGAGTCTGCTGCAATTCAGTGGCCTGTTTGTTGTCTGGCTGCTCTGCACGCTGTTTATTGCCACGCTGACCTGGTTTGAGTTTCGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCtt                                                                                                                               <  2:3028059/150‑1 (MQ=255)

                    ggAGGTCGCATGAGTCTGCTGCAATTCAGTGGCCTGTTTGTTGTCTGGCTGCTCTGCACGCTGTTTATTGCCACGCTGACCTGGTTTGAGTTTCGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCttt                                                                                                                              <  1:4754176/150‑1 (MQ=255)

                    ggAGGTCGCATGAGTCTGCTGCAATTCAGTGGCCTGTTTGTTGTCTGGCTGCTCTGCACGCTGTTTATTGCCACGCTGACCTGGTTTGAGTTTCGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCttt                                                                                                                              >  1:1972490/1‑150 (MQ=255)

                    ggAGGTCGCATGAGTCTGCTGCAATTCAGTGGCCTGTTTGTTGTCTGGCTGCTCTGCACGCTGTTTATTGCCACGCTGACCTGGTTTGAGTTTCGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCttt                                                                                                                              <  2:1885667/150‑1 (MQ=255)

                     gAGGTCGCATGAGTCTGCTGCAATTCAGTGGCCTGTTTGTTGTCTGGCTGCTCTGCACGCTGTTTATTGCCACGCTGACCTGGTTTGAGTTTCGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCtttt                                                                                                                             <  2:3313/150‑1 (MQ=255)

                      aGGTCGCATGAGTCTGCTGCAATTCAGTGGCCTGTTTGTTGTCTGGCTGCTCTGCACGCTGTTTATTGCCACGCTGACCTGGTTTGAGTTTCGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCttttt                                                                                                                            <  2:3197839/150‑1 (MQ=255)

                          cGCATGAGTCTGCTGCAATTCAGTGGCCTGTTTGTTGTCTGGCTGCTCTGCAAGCTGTTTATTGCCACGCTGCCCTGGTTTGAGTTTCGCCGTGGGGGCTTTAACTTCAATGTCTTCTTTTTATTGATGTTTTTGCTCACCTTTttcttc                                                                                                                        >  2:3059127/1‑150 (MQ=255)

                              tGAGTCTGCTGCAATTCAGTGGCCTGTTTGTTGTCTGGCTGCTCTGCACGCTGTTTATTGCCACGCTGACCTGGTTTGAGTTTCGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCt                                                                                                                    <  1:4159703/150‑1 (MQ=255)

                              tGAGTCTGCTGCAATTCAGTGGCCTGTTTGTTGTCTGGCTGCTCTGCACGCTGTTTATTGCCACGCTGACCTGGTTTGAGTTTCGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCt                                                                                                                    >  2:4289128/1‑150 (MQ=255)

                               gAGTCTGCTGCAATTCAGTGGCCTGTTTGTTGTCTGGCTGCTCTGCACGCTGTTTATTGCCACGCTGACCTGGTTTGAGTTTCGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCtt                                                                                                                   <  2:2721171/150‑1 (MQ=255)

                                aGTCTGCTGCAATTCAGTGGCCTGTTTGTTGTCTGGCTGCTCTGCACGCTGTTTATTGCCACGCTGACCTGGTTTGAGTTTCGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTc                                                                                                                  >  2:4766450/1‑150 (MQ=255)

                                aGTCTGCTGCAATTCAGTGGCCTGTTTGTTGTCTGGCTGCTCTGCACGCTGTTTATTGCCACGCTGACCTGGTTTGAGTTTCGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTc                                                                                                                  >  2:1484957/1‑150 (MQ=255)

                                 gTCTGCTGCAATTCAGTGGCCTGTTTGTTGTCTGGCTGCTCTGCACGCTGTTTATTGCCACGCTGACCTGGTTTGAGTTTCGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTcc                                                                                                                 <  2:4887761/150‑1 (MQ=255)

                                      ctgcAATTCAGTGGCCTGTTTGTTGTCTGGCTGCTCTGCACGCTGTTTATTGCCACGCTGACCTGGTTTGAGTTTCGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCGCTg                                                                                                            <  1:2320880/150‑1 (MQ=255)

                                      ctgcAATTCAGTGGCCTGTTTGTTGTCTGGCTGCTCTGCACGCTGTTTATTGCCACGCTGACCTGGTTTGAGTTTCGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCGCTg                                                                                                            >  1:431607/1‑150 (MQ=255)

                                        gcAATTCGGTGGCCTGTTTGTTGTCTGGCTGCTCTGCACGCTGTTTATTGCCACGCTGACCTGGTTTGAGTTTCGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCGCTGAc                                                                                                          >  2:974145/1‑150 (MQ=255)

                                             tCAGTGGCCTGTTTGTTGTCTGGCTGCTCTGCACGCTGTTTATTGCCACGCTGACCTGGTTTGAGTTTCGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCAGCTGACCAGCg                                                                                                     <  1:820750/150‑1 (MQ=255)

                                             tCAGAGGCCTGTTTGTTGTCTGGCTGCTCTGCACGCTGTTTATTGCCACGCTGACCTGGTTTGAGTTTCGCCGTGTGCGCTTAAACTGCAATTTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCGCTGACCAGCg                                                                                                     <  2:4949065/150‑1 (MQ=255)

                                                   gCCTGTTTGTTGTCTGGCTGCTCTGCACGCTGTTTATTGCCACGCTGACCTGGTTTGAGTTTCGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCGCTGACCAGCGTGCTgg                                                                                               >  1:2537341/1‑150 (MQ=255)

                                                    ccTGTTTGTTGTCTGGCTGCTCTGCACGCTGTTTATTGCCACGCTGACCTGGTTTGAGTTTCGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCGCTGACCAGCGTGCTGGt                                                                                              <  1:3761612/150‑1 (MQ=255)

                                                     cTGTTTGTTGTCTGGCTGCTCTGCACGCTGTTTATTGCCACGCTGACCTGGTTTGAGTTTCGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCGCTGACCAGCGTGCTGGTa                                                                                             <  1:3066629/150‑1 (MQ=255)

                                                        tttgttGTCTGGCTGCTCTGCACGCTGTTTATTGCCACGCTGACCTGGTTTGAGTTTCGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCGCTGACCAGCGTGCTGGTAttt                                                                                          >  1:3130442/1‑150 (MQ=255)

                                                         ttgttgTCTGGCTGCTCTGCACGCTGTTTATTGCCACGCTGACCTGGTTTGAGTTTCGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCGCTGACCAGCGTGCTGGTATTTc                                                                                         <  1:3518394/150‑1 (MQ=255)

                                                         ttgttgTCTGGCTGCTCTGCACGCTGTTTATTGCCACGCTGACCTGGTTTGAGTTTCGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCGCTGACCAGCGTGCTGGTATTTc                                                                                         <  2:2612060/150‑1 (MQ=255)

                                                         ttgttgTCTGGCTGCTCTGCACGCTGTTTATTGCCACGCTGACCTGGTTTGAGTTTCGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCGCTGACCAGCGTGCTGGTATTTc                                                                                         >  1:4997592/1‑150 (MQ=255)

                                                         ttgttgTCTGGCTGCTCTGCACGCTGTTTATTGCCACGCTGACCTGGTTTGAGTTTCGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCGCTGACCAGCGTGCTGGTATTTc                                                                                         <  1:2041414/150‑1 (MQ=255)

                                                            ttgtCTGGCTGCTCTGCACGCTGTTTATTGCCACGCTGACCTGGTTTGAGTTTCGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCGCTGACCAGCGTGCTGGTATTTCGCt                                                                                      >  2:2429775/1‑150 (MQ=255)

                                                            ttgtCTGGCTGCTCTGCACGCTGTTTATTGCCACGCTGACCTGGTTTGAGTTTCGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCGCTGACCAGCGTGCTGGTATTTCGCt                                                                                      <  2:651991/150‑1 (MQ=255)

                                                             tgtCTGGCTGCTCTGCACGCTGTTTATTGCCACGCTGACCTGGTTTGAGTTTCGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCGCTGACCAGCGTGCTGGTATTTCGCtt                                                                                     <  2:3836907/150‑1 (MQ=255)

                                                             tgtCTGGCTGCTCTGCACGCTGTTTATTGCCACGCTGACCTGGTTTGAGTTTCGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCGCTGACCAGCGTGCTGGTATTTCGCtt                                                                                     >  1:2382351/1‑150 (MQ=255)

                                                              gtCTGGCTGCTCTGCACGCTGTTTATTGCCACGCTGACCTGGTTTGAGTTTCGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCGCTGACCAGCGTGCTGGTATTTCGCttt                                                                                    <  2:811163/150‑1 (MQ=255)

                                                              gtCTGGCTGCTCTGCACGCTGTTTATTGCCACGCTGACCTGGTTTGAGTTTCGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCGCTGACCAGCGTGCTGGTATTTCGCttt                                                                                    >  1:2245882/1‑150 (MQ=255)

                                                                 tGGCTGCTCTGCACGCTGTTTATTGCCACGCTGACCTGGTTTGAGTTTCGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCGCTGACCAGCGTGCTGGTATTTCGCTTTGAt                                                                                 <  1:1611938/150‑1 (MQ=255)

                                                                      gctCTGCACGCTGTTTATTGCCACGCTGACCTGGTTTGAGTTTCGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCGCTGACCAGCGTGCTGGTATTTCGCTTTGATGTTgg                                                                            <  2:1548658/150‑1 (MQ=255)

                                                                       ctctGCACGCTGTTTATTGCCACGCTGACCTGGTTTGAGTTTCGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCCCCTTTTTCTTCGGCTTCCCGCTGACCAGCGTGCTGGTATTTCGCTTTGATGTTGgt                                                                           <  1:1185342/150‑1 (MQ=255)

                                                                        tctGCACGCTGTTTATTGCCACGCTGACCTGGTTTGAGTTTCGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCGCTGACCAGCGTGCTGGTATTTCGCTTTGATGTTGgtg                                                                          >  1:4645992/1‑150 (MQ=255)

                                                                         ctGCACGCTGTTTATTGCCACGCTGACCTGGTTTGAGTTTCGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCGCTGACCAGCGTGCTGGTATTTCGCTTTGATGTTGgtgt                                                                         >  1:3292924/1‑150 (MQ=255)

                                                                           gCACGCTGTTTATTGCCACGCTGACCTGGTTTGAGTTTCGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCGCTGACCAGCGTGCTGGTATTTCGCTTTGATGTTGGTGTcg                                                                       >  1:636588/1‑150 (MQ=255)

                                                                             aCGCTGTTTATTGCCACGCTGACCTGGTTTGAGTTTCGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCGCTGACCAGCGTGCTGGTATTTCGCTTTGATGTTGGTGTcgcg                                                                     <  1:4493889/150‑1 (MQ=255)

                                                                             aCGCTGTTTATTGCCACGCTGACCTGGTTTGAGTTTCGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCGCTGACCAGCGTGCTGGTATTTCGCTTTGATGTTGGTGTcgcg                                                                     >  2:2221290/1‑150 (MQ=255)

                                                                               gCTGTTTATTGCCACGCTGACCTGGTTTGAGTTTCGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCGCTGACCAGCGTGCTGGTATTTCGCTTTGATGTTGGTGTCGcgcc                                                                   <  2:2275062/150‑1 (MQ=255)

                                                                                cTGTTTATTGCCACGCTGACCTGGTTTGAGTTTCGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCGCTGACCAGCGTGCTGGTATTTCGCTTTGATGTTGGTGTCGcgccg                                                                  >  2:365598/1‑150 (MQ=255)

                                                                                  gTTTATTGCCACGCTGACCTGGTTTGAGTTTCGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCGCTGACCAGCGTGCTGGTATTTCGCTTTGATGTTGGTGTCGCgccgcc                                                                <  1:2064102/150‑1 (MQ=255)

                                                                                    ttATTGCCACGCTGACCTGGTTTGAGTTTCGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCGCTGACCAGCGTGCTGGTATTTCGCTTTGATGTTGGTGTCGCGCCGCCAg                                                              >  1:371260/1‑150 (MQ=255)

                                                                                        tGCCACGCTGACCTGGTTTGAGTTTCGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCGCTGACCAGCGTGCTGGTATTTCGCTTTGATGTTGGTGTCGCGCCGCCAGAAAt                                                          >  2:2958386/1‑150 (MQ=255)

                                                                                           cACGCTGACCTGGTTTGAGTTTCGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCGCTGACCAGCGTGCTGGTATTTCGCTTTGATGTTGGTGTCGCGCCGCCAGAAATCtt                                                       <  1:122492/150‑1 (MQ=255)

                                                                                             cGCTGACCTGGTTTGAGTTTCGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCGCTGACCAGCGTGCTGGTATTTCGCTTTGATGTTGGTGTCGCGCCGCCAGAAATCttgt                                                     >  1:3959105/1‑150 (MQ=255)

                                                                                                tGACCTGGTTTGAGTTTCGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCGCTGACCAGCGTGCTGGTATTTCGCTTTGATGTTGGTGTCGCGCCGCCAGAAATCTTGTTGc                                                  >  2:1012099/1‑150 (MQ=255)

                                                                                                tGACCTGGTTTGAGTTTCGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGTTTTTGATCACCTTTTTCTTCGGCTTCCCGCTGACCAGCGTGCTGGTATTTCGCTATGATGTTGGCGTCGCGCCGCCAGAAATCTTGTTGc                                                  >  2:4615668/1‑150 (MQ=255)

                                                                                                 gACCTGGTTTGAGTTTCGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCGCTGACCAGCGTGCTGGTATTTCGCTTTGATGTTGGTGTCGCGCCGCCAGAAATCTTGTTGCa                                                 >  1:3575941/1‑150 (MQ=255)

                                                                                                  aCCCGGTTTGAGTTTCGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCGCTGACCAGCGTGCTGGTATTTCGCTTTGATGTTGGTGTCGCGCCGCCAGAAATCTTGTTGCAg                                                <  2:1083891/150‑1 (MQ=255)

                                                                                                   ccTGGTTTGAGTTTCGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCGCTGACCAGCGTGCTGGTATTTCGCTTTGATGTTGGTGTCGCGCCGCCAGAAATCTTGTTGCAgg                                               <  2:621661/150‑1 (MQ=255)

                                                                                                    cTGGTTTGAGTTTCGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCGCTGACCAGCGTGCTGGTATTTCGCTTTGATGTTGGTGTCGCGCCGCCAGAAATCTTGTTGCAGGc                                              <  1:4909816/150‑1 (MQ=255)

                                                                                                      ggTTTGAGTTTCGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCGCTGACCAGCGTGCTGGTATTTCGCTTTGATGTTGGTGTCGCGCCGCCAGAAATCTTGTTGCAGGCGt                                            <  2:1348687/150‑1 (MQ=255)

                                                                                                      ggTTTGAGTTTCGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCGCTGACCAGCGTGCTGGTATTTCGCTTTGATGTTGGTGTCGCGCCGCCAGAAATCTTGTTGCAGGCGt                                            >  2:4455715/1‑150 (MQ=255)

                                                                                                      ggTTTGAGTTTCGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCGCTGACCAGCGTGCTGGTATTTCGCTTTGATGTTGGTGTCGCGCCGCCAGAAATCTTGTTGCAGGCGt                                            >  1:2723853/1‑150 (MQ=255)

                                                                                                       gtgtGAGTTTCGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCGCTGACCAGCGTGCTGGTATTTCGCTTTGATGTTGGTGTCGCGCCGCCAGAAATCTTGTTGCAGGCGtt                                           <  2:3903556/147‑1 (MQ=255)

                                                                                                       gTTTGAGTTTCGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCGCTGACCAGCGTGCTGGTATTTCGCTTTGATGTTGGTGTCGCGCCGCCAGAAATCTTGTTGCAGGCGtt                                           <  2:1179643/150‑1 (MQ=255)

                                                                                                       gTTTGAGTTTCGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCGCTGACCAGCGTGCTGGTATTTCGCTTTGATGTTGGTGTCGCGCCGCCAGAAATCTTGTTGCAGGCGtt                                           >  1:4557531/1‑150 (MQ=255)

                                                                                                        tttGAGTTTCGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCGCTGACCAGCGTGCTGGTATTTCGCTTTGATGTTGGTGTCGCGCCGCCAGAAATCTTGTTGCAGGCGTTg                                          >  2:2013406/1‑150 (MQ=255)

                                                                                                            aGTTTCGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCGCTGACCAGCGTGCTGGTATTTCGCTTTGATGTTGGTGTCGCGCCGCCAGAAATCTTGTTGCAGGCGTTGCttt                                      >  2:2139550/1‑150 (MQ=255)

                                                                                                              tttCGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCGCTGACCAGCGTGCTGGTATTTCGCTTTGATGTTGGTGTCGCGCCGCCAGAAATCTTGTTGCAGGCGTTGCTTTCt                                    <  1:3394583/150‑1 (MQ=255)

                                                                                                               ttCGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCGCTGACCAGCGTGCTGGTATTTCGCTTTGATGTTGGTGTCGCGCCGCCAGAAATCTTGTTGCAGGCGTTGCTTTCTg                                   <  2:1466621/150‑1 (MQ=255)

                                                                                                               ttCGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCGCTGACCAGCGTGCTGGTATTTCGCTTTGATGTTGGTGTCGCGCCGCCAGAAATCTTGTTGCAGGCGTTGCTTTCTg                                   >  1:2855921/1‑150 (MQ=255)

                                                                                                                  gCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCGCTGACCAGCGTGCTGGTATTTCGCTTTGATGTTGGTGTCGCGCCGCCAGAAATCTTGTTGCTGGCGTTGCTTTCTGCgg                                >  2:2333997/1‑150 (MQ=255)

                                                                                                                    cGTGTGCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCGCTGACCAGCGTGCTGGTATTTCGCTTTGATGTTGGTGTCGCGCCGCCAGAAATCTTGTTGCAGGCGTTGCTTTCTGCGGgc                              <  2:3465099/150‑1 (MQ=255)

                                                                                                                      tgtgCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCGCTGACCAGCGTGCTGGTATTTCGCTTTGATGTTGGTGTCGCGCCGCCAGAAATCTTGTTGCAGGCGTTGCTTTCTGCGGgctg                            >  1:4080201/1‑150 (MQ=255)

                                                                                                                       gtgCGCTTTAACTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCGCTGACCAGCGTGCTGGTATTTCGCTTTGATGTTGGTGTCGCGCCGCCAGAAATCTTGTTGCAGGCGTTGCTTTCTGCGGgctgc                           <  1:143949/150‑1 (MQ=255)

                                                                                                                         gcgcTTTAACTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCGCTGACCAGCGTGCTGGTATTTCGCTTTGATGTTGGTGTCGCGCCGCCAGAAATCTTGTTGCAGGCGTTGCTTTCTGCGGGCTGCtt                         <  1:3235548/150‑1 (MQ=255)

                                                                                                                           gcTTTAACTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCGCTGACCAGCGTGCTGGTATTTCGCTTTGATGTTGGTGTCGCGCCGCCAGAAATCTTGTTGCAGGCGTTGCTTTCTGCGGGCTGCTTCt                       <  1:2487336/150‑1 (MQ=255)

                                                                                                                            cTTTAACTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCGCTGACCAGCGTGCTGGTATTTCGCTTTGATGTTGGTGTCGCGCCGCCAGAAATCTTGTTGCAGGCGTTGCTTTCTGCGGGCTGCTTCTa                      <  2:4177063/150‑1 (MQ=255)

                                                                                                                            cTTTAACTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCGCTGACCAGCGTGCTGGTATTTCGCTTTGATGTTGGTGTCGCGCCGCCAGAAATCTTGTTGCAGGCGTTGCTTTCTGCGGGCTGCTTCTa                      >  1:1870882/1‑150 (MQ=255)

                                                                                                                             tttAACTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCGCTGACCAGCGTGCTGGTATTTCGCTTTGATGTTGGTGTCGCGCCGCCAGAAATCTTGTTGCAGGCGTTGCTTTCTGCGGGCTGCTTCTAc                     <  1:4843306/150‑1 (MQ=255)

                                                                                                                                aaCTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCGCTGACCAGCGTGCTGGTATTTCGCTTTGATGTTGGTGTCGCGCCGCCAGAAATCTTGTTGCAGGCGTTGCTTTCTGCGGGCTGCTTCTAcgcg                  >  2:3156482/1‑150 (MQ=255)

                                                                                                                                  cTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCGCTGACCAGCGTGCTGGTATTTCGCTTTGATGTTGGTGTCGCGCCGCCAGAAATCTTGTTGCAGGCGTTGCTTTCTGCGGGCTGCTTCTACGCGGt                >  1:1643022/1‑150 (MQ=255)

                                                                                                                                  cTTCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCGCTGACCAGCGTGCTGGTATTTCGCTTTGATGTTGGTGTCGCGCCGCCAGAAATCTTGTTGCAGGCGTTGCTTTCTGCGGGCTGCTTCTACGCGGt                >  1:1060877/1‑150 (MQ=255)

                                                                                                                                    tCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCGCTGACCAGCGTGCTGGTATTTCGCTTTGATGTTGGTGTCGCGCCGCCAGAAATCTTGTTGCAGGCGTTGCTTTCTGCGGGCTGCTTCTACGCGGttt              >  2:1632208/1‑150 (MQ=255)

                                                                                                                                    tCAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCGCTGACCAGCGTGCTGGTATTTCGCTTTGATGTTGGTGTCGCGCCGCCAGAAATCTTGTTGCAGGCGTTGCTTTCTGCGGGCTGCTTCTACGCGGttt              >  1:954449/1‑150 (MQ=255)

                                                                                                                                     cAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCGCTGACCAGCGTGCTGGTATTTCGCTTTGATGTTGGTGTCGCGCCGCCAGAAATCTTGTTGCAGGCGTTGCTTTCTGCGGGCTGCTTCTACGCGGTTTa             <  1:4366018/150‑1 (MQ=255)

                                                                                                                                     cAATGTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCGCTGACCAGCGTGCTGGTATTTCGCTTTGATGTTGGTGTCGCGCCGCCAGAAATCTTGTTGCAGGCGTTGCTTTCTGCGGGCTGCTTCTACGCGGTTTa             <  2:143646/150‑1 (MQ=255)

                                                                                                                                        tGTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCGCTGACCAGCGTGCTGGTATTTCGCTTTGATGTTGGTGTCGCGCCGCCAGAAATCTTGTTGCAGGCGTTGCTTTCTGCGGGCTGCTTCTACGCGGTTTACTa          <  1:3880806/150‑1 (MQ=255)

                                                                                                                                         gTCTTCTTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCGCTGACCAGCGTGCTGGTATTTCGCTTTGATGTTGGTGTCGCGCCGCCAGAAATCTTGTTGCAGGCGTTGCTTTCTGCGGGCTGCTTCTACGCGGTTTACTAt         <  1:1002251/150‑1 (MQ=255)

                                                                                                                                          tcttctTTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCGCTGACCAGCGTGCTGGTATTTCGCTTTGATGTTGGTGTCGCGCCGCCAGAAATCTTGTTGCAGGCGTTGCTTTCTGCGGGCTGCTTCTACGCGGTTTACTATg        >  1:4968133/1‑150 (MQ=255)

                                                                                                                                           cttcttTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCGCTGACCAGCGTGCTGGTATTTCGCTTTGATGTTGGTGTCGCGCCGCCAGAAATCTTGTTGCAGGCGTTGCTTTCTGCGGGCTGCTTCTACGCGGTTTACTATGt       >  1:4209206/1‑150 (MQ=255)

                                                                                                                                           cttcttTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCGCTGACCAGCGTGCTGGTATTTCGCTTTGATGTTGGTGTCGCGCCGCCAGAAATCTTGTTGCAGGCGTTGCTTTCTGCGGGCTGCTTCTACGCGGTTTACTATGt       >  2:1820596/1‑150 (MQ=255)

                                                                                                                                              cttTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCGCTGACCAGCGTGCTGGTATTTCGCTTTGATGTTGGTGTCGCGCCGCCAGAAATCTTGTTGCAGGCGTTGCTTTCTGCGGGCTGCTTCTACGCGGTTTACTATGTCAc    >  2:3565365/1‑150 (MQ=255)

                                                                                                                                              cttTTTATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCGCTGACCAGCGTGCTGGTATTTCGCTTTGATGTTGGTGTCGCGCCGCCAGAAATCTTGTTGCAGGCGTTGCTTTCTGCGGGCTGCTTCTACGCGGTTTACTATGTCAc    >  2:882422/1‑150 (MQ=255)

GCGTTGGCGATTGCCGCCAGGGAGGTCGCATGAGTCTGCTGCAATTCAGTGGCCTGTTTGTTGTCTGGCTGCTCTGCACGCTGTTTATTGCCACGCTGACCTGGTTTGAGTTTCGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTCATTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCGCTGACCAGCGTGCTGGTATTTCGCTTTGATGTTGGTGTCGCGCCGCCAGAAATCTTGTTGCAGGCGTTGCTTTCTGCGGGCTGCTTCTACGCGGTTTACTATGTCACCT  >  AP012306/3407785‑3408078

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`