BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AP012306	2,315,375	(A)_5→4	coding (26/1203 nt)	proV →	glycine betaine transporter subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AP012306	2,315,371	0	A	.	100.0%	2625.1 / NA	529	coding (22/1203 nt)	proV	glycine betaine transporter subunit
Reads supporting (aligned to +/- strand): ref base A (0/0); new base . (252/277); total (252/277)

Only 100 of 575 total aligned reads displayed.

CATCAATATTCATGCCACATTTGCCATCAGGGGTTGCCTCAGATTCTCAGTATGTTAGGGTAGAAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTAAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGGAAAAAACTGGGCTATCGCTTGGCGTAAAAGACGCCAGTCTGGCCATTGAAGAAGGCGAGAT  >  AP012306/2315222‑2315516

cATCAATATTCATGCCACATTTGCCATCAGGGGTTGCCTCCGATTCTCAGTATGTTAGGGTAGAAAAAAGGGACTATTTCCATTGGGTAATATATCGACATAAACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTa                                                                                                                                                   >  2:3652814/1‑150 (MQ=255)

 aTCAATATTCATGCCACATTTGCCATCAGGGGTTGCCTCAGATTCTCAGTATGTTAGGGTAGAAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTaa                                                                                                                                                  >  1:2207007/1‑150 (MQ=255)

    aaTATTCATGCCACATTTGCCATCAGGGGTTGCCTCAGATTCTCAGTATGTTAGGGTAGAAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTaaaat                                                                                                                                               >  2:3138794/1‑149 (MQ=255)

     atatTCATGCCACATTTGCCATCAGGGGTTGCCTCAGATTCTCAGTATGTTAGGGTAGAAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTaaaatc                                                                                                                                              <  1:2453253/150‑3 (MQ=255)

       atTCATGCCACATTTGCCATCAGGGGTTGCCTCAGATTCTCAGTATGTTAGGGTAGAAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTaaaatctt                                                                                                                                            <  2:3580049/150‑5 (MQ=255)

         tCATGCCACATTTGCCATCAGGGGTTGCCTCAGATTCTCAGTATGTTAGGGTAGAAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATT‑AAAATCTTta                                                                                                                                         >  2:4626778/1‑150 (MQ=255)

         tCATGCCACATTTGCCATCAGGGGTTGCCTCAGATTCTCAGTATGTTAGGGTAGAAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATT‑AAAATCTTta                                                                                                                                         <  1:498485/150‑1 (MQ=255)

          cATGCCACATTTGCCATCAGGGGTTGCCTCAGATTCTCAGTATGTTAGGGTAGAAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATT‑AAAATCTTtat                                                                                                                                        <  1:4010205/150‑1 (MQ=255)

            tGCCACATTTGCCATCAGGGGTTGCCTCAGATTCTCAGTATGTTAGGGTAGAAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATT‑AAAATCTTTATaa                                                                                                                                      >  2:2283942/1‑150 (MQ=255)

            tGCCACATTTGCCATCAGGGGTTGCCTCAGATTCTCAGTATGTTAGGGTAGAAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATT‑AAAATCTTTATaa                                                                                                                                      <  1:3789842/150‑1 (MQ=255)

            tGCAACATTTGCCATCAGGGGTTGCCTCAGATTCTCAGTATGTTAGGGTAGAAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATT‑AAAATCTTTATaa                                                                                                                                      <  1:2821090/150‑1 (MQ=255)

              ccACATTTGCCATCAGGGGTTGCCTCAGATTCTCAGTATGTTAGGGTAGAAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATT‑AAAATCTTTATaaaa                                                                                                                                    >  2:64713/1‑150 (MQ=255)

                 caTTTGCCATCAGGGGTTGCCTCAGATTCTCAGTATGTTAGGGTAGAAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATT‑AAAATCTTTATAAAatat                                                                                                                                 >  1:2401496/1‑150 (MQ=255)

                  aTTTGCCATCAGGGGTTGCCTCAGATTCTCAGTATGTTAGGGTAGAAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATT‑AAAATCTTTATAAAATAtt                                                                                                                                <  1:667751/150‑1 (MQ=255)

                    ttGCCATCAGGGGTTGCCTCAGATTCTCAGTATGTTAGGGTAGAAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATT‑AAAATCTTTATAAAATATTTg                                                                                                                              <  2:4076631/150‑1 (MQ=255)

                            aGGGGTTGCCTCAGATTCTCAGTATGTTAGGGTAGAAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATT‑AAAATCTTTATAAAATATTTGGCGAGCAt                                                                                                                      >  1:4280421/1‑150 (MQ=255)

                                gTTGCCTCAGATTCTCAGTATGTTAGGGTAGAAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATT‑AAAATCTTTATAAAATATTTGGCGAGCATCcac                                                                                                                  >  1:3684250/1‑150 (MQ=255)

                                  tGCCTCAGATTCTCAGTATGTTAGGGTAGAAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATT‑AAAATCTTTATAAAATATTTGGCGAGCATCCACag                                                                                                                >  1:2033020/1‑150 (MQ=255)

                                     cTCAGATTCTCAGTATGTTAGGGTAGAAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATT‑AAAATCTTTATAAAATATTTGGCGAGCATCCACagcga                                                                                                             >  1:4082166/1‑150 (MQ=255)

                                         gATTCTCAGTATGTTAGGGTAGAAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATT‑AAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGt                                                                                                         >  2:10784/1‑150 (MQ=255)

                                            tctcAGTATGTTAGGGTAGAAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATT‑AAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCa                                                                                                      >  1:1013523/1‑150 (MQ=255)

                                            tctcAGTATGTTAGGGGAGAAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATT‑AAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCa                                                                                                      >  2:1526600/1‑150 (MQ=255)

                                               cAGTATGTTAGGGTAGAAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATT‑AAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAat                                                                                                   <  2:1018811/150‑1 (MQ=255)

                                                aGTATGTTAGGGTAGAAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATT‑AAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAata                                                                                                  >  2:2052708/1‑150 (MQ=255)

                                                aGTATGTTAGGGTAGAAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATT‑AAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAata                                                                                                  <  2:2088304/150‑1 (MQ=255)

                                                aGTATGTTAGGGTAGAAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATT‑AAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAata                                                                                                  <  1:1506289/150‑1 (MQ=255)

                                                 gTATGTTAGGGTAGAAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATT‑AAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAatat                                                                                                 >  2:3529270/1‑150 (MQ=255)

                                                   aTGTTAGGGTAGAAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATT‑AAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAatatat                                                                                               >  1:2278906/1‑150 (MQ=255)

                                                     gTTAGGGTAGAAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATT‑AAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCg                                                                                             >  1:4807861/1‑150 (MQ=255)

                                                     gTTAGGGTAGAAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATT‑AAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCg                                                                                             <  1:3898687/150‑1 (MQ=255)

                                                     gTTAGGGTAGAAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATT‑AAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCg                                                                                             <  1:1084142/150‑1 (MQ=255)

                                                      ttAGGGTAGAAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATT‑AAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGa                                                                                            >  2:2689268/1‑150 (MQ=255)

                                                         gggTAGAAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATT‑AAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACa                                                                                         >  2:4144846/1‑150 (MQ=255)

                                                         gggTAGAAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATT‑AAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACa                                                                                         >  2:683190/1‑150 (MQ=255)

                                                          ggTAGAAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATT‑AAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACaa                                                                                        <  2:3458308/150‑1 (MQ=255)

                                                           gTAGAAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATT‑AAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAg                                                                                       >  1:2703811/1‑150 (MQ=255)

                                                           gTAGAAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATT‑AAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAg                                                                                       <  1:4389857/150‑1 (MQ=255)

                                                             aGAAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATT‑AAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGa                                                                                     >  1:2641176/1‑150 (MQ=255)

                                                              gAAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATT‑AAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGAc                                                                                    >  2:2562932/1‑150 (MQ=255)

                                                                  aaaGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATT‑AAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTc                                                                                >  1:1442992/1‑150 (MQ=255)

                                                                       gACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATT‑AAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAg                                                                           <  1:744860/150‑1 (MQ=255)

                                                                        aCTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATT‑AAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGa                                                                          <  2:582624/150‑1 (MQ=255)

                                                                        aCTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATT‑AAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGa                                                                          <  2:339175/150‑1 (MQ=255)

                                                                         cTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATT‑AAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGaa                                                                         >  2:3885617/1‑150 (MQ=255)

                                                                         cTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATT‑AAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGaa                                                                         >  2:445622/1‑150 (MQ=255)

                                                                          tATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATT‑AAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAAc                                                                        <  2:3986402/150‑1 (MQ=255)

                                                                          tATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATT‑AAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAAc                                                                        <  1:1257220/150‑1 (MQ=255)

                                                                             ttCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATT‑AAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACaaa                                                                     >  2:4406263/1‑150 (MQ=255)

                                                                              tCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATT‑AAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAAt                                                                    >  2:1985250/1‑150 (MQ=255)

                                                                                cATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATT‑AAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTc                                                                  <  2:192261/150‑1 (MQ=255)

                                                                                cATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATT‑AAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTc                                                                  <  1:3570371/150‑1 (MQ=255)

                                                                                 aTTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATT‑AAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCt                                                                 <  2:1650731/150‑1 (MQ=255)

                                                                                 aTTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATT‑AAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCt                                                                 <  2:1318575/150‑1 (MQ=255)

                                                                                    gggTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATT‑AAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGGa                                                              >  2:2835857/1‑150 (MQ=255)

                                                                                      gTAATATATCGATATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATT‑AAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGGaaa                                                            >  2:4883226/1‑150 (MQ=255)

                                                                                      gTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATT‑AAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGGaaa                                                            >  2:3836788/1‑150 (MQ=255)

                                                                                      gTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATT‑AAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGGaaa                                                            >  1:252993/1‑150 (MQ=255)

                                                                                         atatatCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATT‑AAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGGaaaaaa                                                         <  1:1856526/150‑1 (MQ=255)

                                                                                         atatatCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATT‑AAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGGaaaaaa                                                         >  2:2967098/1‑150 (MQ=255)

                                                                                         atatatCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATT‑AAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGGaaaaaa                                                         >  1:4503154/1‑150 (MQ=255)

                                                                                           atatCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATT‑AAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGGAAAAAACt                                                       <  1:422592/150‑1 (MQ=255)

                                                                                            tatCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATT‑AAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGGAAAAAACTg                                                      >  1:962272/1‑150 (MQ=255)

                                                                                               cGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATT‑AAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGGAAAAAACTGGGc                                                   >  2:3085314/1‑150 (MQ=255)

                                                                                                gACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATT‑AAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGGAAAAAACTGGGCt                                                  >  2:1149476/1‑150 (MQ=255)

                                                                                                 aCATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATT‑AAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGGAAAAAACTGGGCTa                                                 <  2:848268/150‑1 (MQ=255)

                                                                                                    tAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATT‑AAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGGAAAAAACTGGGCTATCg                                              >  2:3764956/1‑150 (MQ=255)

                                                                                                       aCAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATT‑AAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGGAAAAAACTGGGCTATCGCtt                                           >  1:208772/1‑150 (MQ=255)

                                                                                                         aaaTAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATT‑AAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGGAAAAAACTGGGCTATCGCTTgg                                         >  1:3413576/1‑150 (MQ=255)

                                                                                                          aaTAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATT‑AAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGGAAAAAACTGGGCTATCGCTTGGc                                        <  1:4666708/150‑1 (MQ=255)

                                                                                                          aaTAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATT‑AAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGGAAAAAACTGGGCTATCGCTTGGc                                        >  1:3745338/1‑150 (MQ=255)

                                                                                                              aaGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATT‑AAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGGAAAAAACTGGGCTATCGCTTGGCGTaa                                    >  1:864482/1‑150 (MQ=255)

                                                                                                               aGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATT‑AAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGGAAAAAACTGGGCTATCGCTTGGCGTaaa                                   >  2:4258508/1‑150 (MQ=255)

                                                                                                                ggAATCTTTCTATTGCATGGCAATTAAATTAGAAATT‑AAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGGAAAAAACTGGGCTATCGCTTGGCGTaaaa                                  >  1:1939396/1‑150 (MQ=255)

                                                                                                                ggAATCTTTCTATTGCATGGCAATTAAATTAGAAATT‑AAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGGAAAAAACTGGGCTATCGCTTGGCGTaaaa                                  >  1:3369175/1‑150 (MQ=255)

                                                                                                                ggAATCTTTCTATTGCATGGCAATTAAATTAGAAATT‑AAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGGAAAAAACTGGGCTATCGCTTGGCGTaaaa                                  >  2:1737857/1‑150 (MQ=255)

                                                                                                                 gAATCTTTCTATTGCATGGCAATTAAATTAGAAATT‑AAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGGAAAAAACTGGGCTATCGCTTGGCGTAAAAg                                 >  1:4852783/1‑150 (MQ=255)

                                                                                                                  aaTCTTTCTATTGGATGGCAATTAAATTAGAAATT‑AAAATCTTTATAAAATATTTTGCGAGCATCCACAGCGAGCGATCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGGAAAAAACTGGGCTATCGCTTGGCGTAAAAGa                                >  2:2175974/1‑150 (MQ=255)

                                                                                                                  aaTCTTTCTATTGCATGGCAATTAAATTAGAAATT‑AAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGGAAAAAACTGGGCTATCGCTTGGCGTAAAAGa                                >  1:794357/1‑150 (MQ=255)

                                                                                                                   aTCTTTCTATTGCATGGCAATTAAATTAGAAATT‑AAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGGAAAAAACTGGGCTATCGCTTGGCGTAAAAGAc                               <  2:2126315/150‑1 (MQ=255)

                                                                                                                   aTCTTTCTATTGCATGGCAATTAAATTAGAAATT‑AAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGGAAAAAACTGGGCTATCGCTTGGCGTAAAAGAc                               >  2:4583012/1‑150 (MQ=255)

                                                                                                                    tCTTTCTATTGCATGGCAATTAAATTAGAAATT‑AAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGGAAAAAACTGGGCTATCGCTTGGCGTAAAAGACg                              >  2:4755590/1‑150 (MQ=255)

                                                                                                                     cTTTCTATTGCATGGCAATTAAATTAGAAATT‑AAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGGAAAAAACTGGGCTATCGCTTGGCGTAAAAGACGc                             <  1:3052897/150‑1 (MQ=255)

                                                                                                                      tttCTATTGCATGGCAATTAAATTAGAAATT‑AAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGGAAAAAACTGGGCTATCGCTTGGCGTAAAAGACGcc                            <  1:2900004/150‑1 (MQ=255)

                                                                                                                       ttCTATTGCATGGCAATTAAATTAGAAATT‑AAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGGAAAAAACTGGGCTATCGCTTGGCGTAAAAGACGCCa                           >  2:3708754/1‑150 (MQ=255)

                                                                                                                        tCTATTGCATGGCAATTAAATTAGAAATT‑AAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGGAAAAAACTGGGCTATCGCTTGGCGTAAAAGACGCCAg                          <  1:443479/150‑1 (MQ=255)

                                                                                                                         cTATTGCATGGCAATTAAATTAGAAATT‑AAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGGAAAAAACTGGGCTATCGCTTGGCGTAAAAGACGCCAGt                         >  1:294584/1‑150 (MQ=255)

                                                                                                                            ttGCATGGCAATTAAATTAGAAATT‑AAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGGAAAAAACTGGGCTATCGCTTGGCGTAAAAGACGCCAGTCTg                      <  1:1759018/150‑1 (MQ=255)

                                                                                                                             tGCATGGCAATTAAATTAGAAATT‑AAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGGAAAAAACTGGGCTATCGCTTGGCGTAAAAGACGCCAGTCTgg                     >  2:813444/1‑150 (MQ=255)

                                                                                                                                 tGGCAATTAAATTAGAAATT‑AAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGGAAAAAACTGGGCTATCGCTTGGCGTAAAAGACGCCAGTCTGGCCAt                 >  1:3525078/1‑150 (MQ=255)

                                                                                                                                 tGGCAATTAAATTAGAAATT‑AAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGGAAAAAACTGGGCTATCGCTTGGCGTAAAAGACGCCAGTCTGGCCAt                 <  2:2724570/150‑1 (MQ=255)

                                                                                                                                 tGGCAATTAAATTAGAAATT‑AAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGGAAAAAACTGGGCTATCGCTTGGCGTAAAAGACGCCAGTCTGGCCAt                 <  1:3283097/150‑1 (MQ=255)

                                                                                                                                   gCAATTAAATTAGAAATT‑AAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGGAAAAAACTGGGCTATCGCTTGGCGTAAAAGACGCCAGTCTGGCCATTg               <  2:145926/150‑1 (MQ=255)

                                                                                                                                   gCAATTAAATTAGAAATT‑AAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGGAAAAAACTGGGCTATCGCTTGGCGTAAAAGACGCCAGTCTGGCCATTg               >  2:2412325/1‑150 (MQ=255)

                                                                                                                                        taaattaGAAATT‑AAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGGAAAAAACTGGGCTATCGCTTGGCGTAAAAGACGCCAGTCTGGCCATTgaagaa          <  1:314565/150‑1 (MQ=255)

                                                                                                                                         aaattaTAAATT‑AAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGGAAAAAACTGGGCTATCGCTTGGCGTAAAAGACGCCAGTCTGGCCATTGaagaag         >  1:2156576/1‑150 (MQ=255)

                                                                                                                                         aaattaGAAATT‑AAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGGAAAAAACTGGGCTATCGCTTGGCGTAAAAGACGCCAGTCTGGCCATTGaagaag         >  2:4356401/1‑150 (MQ=255)

                                                                                                                                             taGAAATT‑AAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGGAAAAAACTGGGCTATCGCTTGGCGTAAAAGACGCCAGTCTGGCCATTGAAGAAGGCga     >  2:3294792/1‑150 (MQ=255)

                                                                                                                                              aGAAATT‑AAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGGAAAAAACTGGGCTATCGCTTGGCGTAAAAGACGCCAGTCTGGCCATTGAAGAAGGCgag    <  1:4570803/150‑1 (MQ=255)

                                                                                                                                               gAAATT‑AAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGGAAAAAACTGGGCTATCGCTTGGCGTAAAAGACGCCAGTCTGGCCATTGAAGAAGGCgaga   <  1:1189807/150‑1 (MQ=255)

                                                                                                                                                aaaTT‑AAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGGAAAAAACTGGGCTATCGCTTGGCGTAAAAGACGCCAGTCTGGCCATTGAAGAAGGCGAGat  <  1:608154/150‑1 (MQ=255)

CATCAATATTCATGCCACATTTGCCATCAGGGGTTGCCTCAGATTCTCAGTATGTTAGGGTAGAAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTAAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGGAAAAAACTGGGCTATCGCTTGGCGTAAAAGACGCCAGTCTGGCCATTGAAGAAGGCGAGAT  >  AP012306/2315222‑2315516

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`