BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	AP012306	1,438,376	Δ719 bp		[pykF]–[lpp]	[pykF], [lpp]

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	AP012306	1438376	1439094	719	367 [0]	[0] 370	[pykF]–[lpp]	[pykF],[lpp]

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	AP012306	= 1438375	0 (0.000)	360 (1.010)	205/296	0.2	100%	coding (1095/1413 nt)	pykF	pyruvate kinase I
*	?	AP012306	1439095 =	0 (0.000)	360 (1.010)	205/296	0.2	100%	coding (92/237 nt)	lpp	murein lipoprotein

Only 100 of 370 total aligned reads displayed.

CTTTGTGTAATACTTGTAACGCTACATGGAGATTAACTCAATCTAGAGGGTATTAATAATGAAAGCTACTAAACTGGTACTGGGCGCGGTAATCCTGGGTTCTACTCTGCTGGCAGGTTGCTCCAGCAACGCTAAAATCGATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAAAGTTGACCAGCTGAGCAACGACGTGAACGCAATGCGTTCCGACGTTCAGGCTGCTAAAGATGACGCAGCTCGTGCTAACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAATAGT  >  AP012306/1438946‑1439244

caaaaggtaaatacccgctggaagcggtttctatcatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttcaacaatgacaaccgtaaactgcgcattaccgaagcggtatgccgtggtgccgttgaaactc                                                                                                                                                       >  1:2384071‑M2/150‑150 (MQ=255)

caaaaggtaaatacccgctggaagcggtttctatcatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttcaacaatgacaaccgtaaactgcgcattaccgaagcggtatgccgtggtgccgttgaaactc                                                                                                                                                       >  2:3255796‑M2/150‑150 (MQ=255)

caaaaggtaaatacccgctggaagcggtttctatcatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttcaacaatgacaaccgtaaactgcgcattaccgaagcggtatgccgtggtgccgttgaaactc                                                                                                                                                       <  1:200395‑M2/1‑1 (MQ=255)

  aaaggtaaatacccgctggaagcggtttctatcatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttcaacaatgacaaccgtaaactgcgcattaccgaagcggtatgccgtggtgccgttgaaactctt                                                                                                                                                     <  1:2804937‑M2/3‑1 (MQ=255)

      gtaaatacccgctggaagcggtttctatcatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttcaacaatgacaaccgtaaactgcgcattaccgaagcggtatgccgtggtgccgttgaaactCTTCTGa                                                                                                                                                 <  1:3719485‑M2/7‑1 (MQ=255)

       taaatacccgctggaagcggtttctatcatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttcaacaatgacaaccgtaaactgcgcattaccgaagcggtatgccgtggtgccgttgaaactCTTCTGAc                                                                                                                                                <  2:3907532‑M2/8‑1 (MQ=255)

        aaatacccgctggaagcggtttctatcatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttcaacaatgacaaccgtaaactgcgcattaccgaagcggtatgccgtggtgccgttgaaactCTTCTGACg                                                                                                                                               >  2:1382991‑M2/142‑150 (MQ=255)

          atacccgctggaagcggtttctatcatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttcaacaatgacaaccgtaaactgcgcattaccgaagcggtatgccgtggtgccgttgaaactCTTCTGACGtt                                                                                                                                             >  2:2027347‑M2/140‑150 (MQ=255)

            acccgctggaagcggtttctatcatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttcaacaatgacaaccgtaaactgcgcattaccgaagcggtatgccgtggtgccgttgaaactCTTCTGACGTTCa                                                                                                                                           <  1:3990508‑M2/13‑1 (MQ=255)

            acccgctggaagcggtttctatcatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttcaacaatgacaaccgtaaactgcgcattaccgaagcggtatgccgtggtgccgttgaaactCTTCTGACGTTCa                                                                                                                                           <  2:91218‑M2/13‑1 (MQ=255)

            acccgctggaagcggtttctatcatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttcaacaatgacaaccgtaaactgcgcattaccgaagcggtatgccgtggtgccgttgaaactCCTCTGACGTTCa                                                                                                                                           <  1:972722‑M2/13‑1 (MQ=255)

             cccgctggaagcggtttctatcatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttcaacaatgacaaccgtaaactgcgcattaccgaagcggtatgccgtggtgccgttgaaactCTTCTGACGTTCAg                                                                                                                                          >  1:3915734‑M2/137‑150 (MQ=255)

             cccgctggaagcggtttctatcatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttcaacaatgacaaccgtaaactgcgcattaccgaagcggtatgccgtggtgccgttgaaactCTTCTGACGTTCAg                                                                                                                                          <  1:2510744‑M2/14‑1 (MQ=255)

               cgctggaagcggtttctatcatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttcaacaatgacaaccgtaaactgcgcattaccgaagcggtatgccgtggtgccgttgaaactCTTCTGACGTTCAGAc                                                                                                                                        <  1:2946723‑M2/16‑1 (MQ=255)

                 ctggaagcggtttctatcatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttcaacaatgacaaccgtaaactgcgcattaccgaagcggtatgccgtggtgccgttgaaactCTTCTGACGTTCAGActc                                                                                                                                      <  2:2777213‑M2/18‑1 (MQ=255)

                 ctggaagcggtttctatcatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttcaacaatgacaaccgtaaactgcgcattaccgaagcggtatgccgtggtgccgttgaaactCTTCTGACGTTCAGActc                                                                                                                                      <  1:2605665‑M2/18‑1 (MQ=255)

                  tggaagcggtttctatcatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttcaacaatgacaaccgtaaactgcgcattaccgaagcggtatgccgtggtgccgttgaaactCTTCTGACGTTCAGActct                                                                                                                                     <  2:3669460‑M2/19‑1 (MQ=255)

                   ggaagcggtttctatcatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttcaacaatgacaaccgtaaactgcgcattaccgaagcggtatgccgtggtgccgttgaaactCTTCTGACGTTCAGACTCTg                                                                                                                                    >  1:1122817‑M2/131‑150 (MQ=255)

                    gaagcggtttctatcatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttcaacaatgacaaccgtaaactgcgcattaccgaagcggtatgccgtggtgccgttgaaactCTTCTGACGTTCAGACTCTGa                                                                                                                                   <  1:1269892‑M2/21‑1 (MQ=255)

                    gaagcggtttctatcatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttcaacaatgacaaccgtaaactgcgcattaccgaagcggtatgccgtggtgccgttgaaactCTTCTGACGTTCAGACTCTGa                                                                                                                                   <  1:4230477‑M2/21‑1 (MQ=255)

                     aagcggtttctatcatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttcaacaatgacaaccgtaaactgcgcattaccgaagcggtatgccgtggtgccgttgaaactCTTCTGACGTTCAGACTCTGaa                                                                                                                                  <  1:2473400‑M2/22‑1 (MQ=255)

                      agcggtttctatcatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttcaacaatgacaaccgtaaactgcgcattaccgaagcggtatgccgtggtgccgttgaaactCTTCTGACGTTCAGACTCTGAAc                                                                                                                                 >  2:4714794‑M2/128‑150 (MQ=255)

                       gcggtttctatcatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttcaacaatgacaaccgtaaactgcgcattaccgaagcggtatgccgtggtgccgttgaaactCTTCTGACGTTCAGACTCTGAACg                                                                                                                                >  2:4075392‑M2/127‑150 (MQ=255)

                           tttctatcatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttcaacaatgacaaccgtaaactgcgcattaccgaagcggtatgccgtggtgccgttgaaactCTTCTGACGTTCAGACTCTGAACGCTaa                                                                                                                            <  2:3789649‑M2/28‑1 (MQ=255)

                            ttctatcatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttcaacaatgacaaccgtaaactgcgcattaccgaagcggtatgccgtggtgccgttgaaactCTTCTGACGTTCAGACTCTGAACGCTaaa                                                                                                                           <  2:3766381‑M2/29‑1 (MQ=255)

                             tctatcatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttcaacaatgacaaccgtaaactgcgcattaccgaagcggtatgccgtggtgccgttgaaactCTTCTGACGTTCAGACTCTGAACGCTAAAg                                                                                                                          <  1:3107028‑M2/30‑1 (MQ=255)

                              ctatcatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttcaacaatgacaaccgtaaactgcgcattaccgaagcggtatgccgtggtgccgttgaaactCTTCTGACGTTCAGACTCTGAACGCTAAAGt                                                                                                                         >  1:2436252‑M2/120‑150 (MQ=255)

                              ctatcatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtcgcgagttcaacaatcacaaccgtaaactgcgcattaccgaagcggtatgccgtggtgccgttgaaactCTTCTGACGTTCAGACTCTGAACGCTAAAGt                                                                                                                         <  2:1773896‑M2/31‑1 (MQ=255)

                               tatcatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttcaacaatgacaaccgtaaactgcgcattaccgaagcggtatgccgtggtgccgttgaaactCTTCTGACGTTCAGACTCTGAACGCTAAAGtt                                                                                                                        <  2:71063‑M2/32‑1 (MQ=255)

                               tatcatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctccggttcaacaatgacaaccgtaaactgcgcattaccgaagcggtatgccgtggtgccgttgaaactCTTCTCACGTTCACACTCTGAACGCTAAAGtt                                                                                                                        >  1:837048‑M2/119‑150 (MQ=255)

                                  catggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttcaacaatgacaaccgtaaactgcgcattaccgaagcggtatgccgtggtgccgttgaaactCTTCTGACGTTCAGACTCTGAACGCTAAAGTTGAc                                                                                                                     >  2:1118807‑M2/116‑150 (MQ=255)

                                   atggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttcaacaatgacaaccgtaaactgcgcattaccgaagcggtatgccgtggtgccgttgaaactCTTCTGACGTTCAGACTCTGAACGCTAAAGTTGAcc                                                                                                                    >  1:2152180‑M2/115‑150 (MQ=255)

                                    tggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttcaacaatgacaaccgtaaactgcgcattaccgaagcggtatgccgtggtgccgttgaaactCTTCTGACGTTCAGACTCTGAACGCTAAAGTTGACCa                                                                                                                   >  1:740440‑M2/114‑150 (MQ=255)

                                     ggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttcaacaatgacaaccgtaaactgcgcattaccgaagcggtatgccgtggtgccgttgaaactCTTCTGACGTTCAGACTCTGAACGCTAAAGTTGACCAg                                                                                                                  >  1:1015406‑M2/113‑150 (MQ=255)

                                      gcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttcaacaatgacaaccgtaaactgcgcattaccgaagcggtatgccgtggtgccgttgaaactCTTCTGACGTTCAGACTCTGAACGCTAAAGTTGACCAGc                                                                                                                 >  2:1652875‑M2/112‑150 (MQ=255)

                                       cgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttcaacaatgacaaccgtaaactgcgcattaccgaagcggtatgccgtggtgccgttgaaactCTTCTGACGTTCAGACTCTGAACGCTAAAGTTGACCAGCt                                                                                                                <  2:1473194‑M2/40‑1 (MQ=255)

                                          ccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttcaacaatgacaaccgtaaactgcgcattaccgaagcggtatgccgtggtgccgttgaaactCTTCTGACGTTCAGACTCTGAACGCTAAAGTTGACCAGCTGAg                                                                                                             <  1:3477755‑M2/43‑1 (MQ=255)

                                           catctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttcaacaatgacaaccgtaaactgcgcattaccgaagcggtatgccgtggtgccgttgaaactCTTCTGACGTTCAGACTCTGAACGCTAAAGTTGACCAGCTGAGc                                                                                                            >  2:534234‑M2/107‑150 (MQ=255)

                                              ctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttcaacaatgacaaccgtaaactgcgcattaccgaagcggtatgccgtggtgccgttgaaactCTTCTGACGTTCAGACTCTGAACGCTAAAGTTGACCAGCTGAGCAac                                                                                                         <  1:2074827‑M2/47‑1 (MQ=255)

                                               tgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttcaacaatgacaaccgtaaactgcgcattaccgaagcggtatgccgtggtgccgttgaaactCTTCTGACGTTCAGACTCTGAACGCTAAAGTTGACCAGCTGAGCAacg                                                                                                        >  1:453044‑M2/103‑150 (MQ=255)

                                                 cgaacgtaccgaccgcgtgatgaacagccgtctcgagttcaacaatgacaaccgtaaactgcgcattaccgaagcggtatgccgtggtgccgttgaaactCTTCTGACGTTCAGACTCTGAACGCTAAAGTTGACCAGCTGAGCAacgac                                                                                                      >  2:1815042‑M2/101‑150 (MQ=255)

                                                 cgaacgtaccgaccgcgtgatgaacagccgtctcgagttcaacaatgacaaccgtaaactgcgcattaccgaagcggtatgccgtggtgccgttgaaactCTTCTGACGTTCAGACTCTGAACGCTAAAGTTGACCAGCTGAGCAacgac                                                                                                      <  2:4211402‑M2/50‑1 (MQ=255)

                                                  gaacgtaccgaccgcgtgatgaacagccgtctcgagttcaacaatgacaaccgtaaactgcgcattaccgaagcggtatgccgtggtgccgttgaaactCTTCTGACGTTCAGACTCTGAACGCTAAAGTTGACCAGCTGAGCAacgacg                                                                                                     <  2:1581572‑M2/51‑1 (MQ=255)

                                                    acgtaccgaccgcgtgatgaacagccgtctcgagttcaacaatgacaaccgtaaactgcgcattaccgaagcggtatgccgtggtgccgttgaaactCTTCTGACGTTCAGACTCTGAACGCTAAAGTTGACCAGCTGAGCAACGACGTg                                                                                                   <  2:1264784‑M2/53‑1 (MQ=255)

                                                      gtaccgaccgcgtgatgaacagccgtctcgagttcaacaatgacaaccgtaaactgcgcattaccgaagcggtatgccgtggtgccgttgaaactCTTCTGACGTTCAGACTCTGAACGCTAAAGTTGACCAGCTGAGCAACGACGTGaa                                                                                                 <  2:4585525‑M2/55‑1 (MQ=255)

                                                        accgaccgcgtgatgaacagccgtctcgagttcaacaatgacaaccgtaaactgcgcattaccgaagcggtatgccgtggtgccgttgaaactCTTCTGACGTTCAGACTCTGAACGCTAAAGTTGACCAGCTGAGCAACGACGTGAACg                                                                                               <  2:3495182‑M2/57‑1 (MQ=255)

                                                            accgcgtgatgaacagccgtctcgagttcaacaatgacaaccgtaaactgcgcattaccgaagcggtatgccgtggtgccgttgaaactCTTCTGACGTTCAGACTCTGAACGCTAAAGTTGACCAGCTGGGCAACGACGTGAACGCAAt                                                                                           <  2:758889‑M2/61‑1 (MQ=255)

                                                             ccgcgtgatgaacagccgtctcgagttcaacaatgacaaccgtaaactgcgcattaccgaagcggtatgccgtggtgccgttgaaactCTTCTGACGTTCAGACTCTGAACGCTAAAGTTGACCAGCTGAGCAACGACGTGAACGCAATg                                                                                          <  2:34198‑M2/62‑1 (MQ=255)

                                                             ccgcgtgatgaacagccgtctcgagttcaacaatgacaaccgtaaactgcgcattaccgaagcggtatgccggggtgccgttgaaactCTTCTGACGTTCAGACTCTGAACGCTAAAGTTGACCAGCTGAGCAACGACGTGAACGCAATg                                                                                          >  1:4855787‑M2/89‑150 (MQ=255)

                                                              cgcgtgatgaacagccgtctcgagttcaacaatgacaaccgtaaactgcgcattaccgaagcggtatgccgtggtgccgttgaaactCTTCTGACGTTCAGACTCTGAACGCTAAAGTTGACCAGCTGAGCAACGACGTGAACGCAATGc                                                                                         >  2:2519609‑M2/88‑150 (MQ=255)

                                                                cgtgatgaacagccgtctcgagttcaacaatgacaaccgtaaactgcgcattaccgaagcggtatgccgtggtgccgttgaaactCTTCTGACGTTCAGACTCTGAACGCTAAAGTTGACCAGCTGAGCAACGACGTGAACGCAATGCGt                                                                                       <  1:3076123‑M2/65‑1 (MQ=255)

                                                                 gtgatgaacagccgtctcgagttcaacaatgacaaccgtaaactgcgcattaccgaagcggtatgccgtggtgccgttgaaactCTTCTGACGTTCAGACTCTGAACGCTAAAGTTGACCAGCTGAGCAACGACGTGAACGCAATGCGtt                                                                                      <  1:1822491‑M2/66‑1 (MQ=255)

                                                                   gatgaacagccgtctcgagttcaacaatgacaaccgtaaactgcgcattaccgaagcggtatgccgtggtgccgttgaaactCTTCTGACGTTCAGACTCTGAACGCTAAAGTTGACCAGCTGAGCAACCACGTGAACGCAATGCGTTcc                                                                                    >  1:3803869‑M2/83‑150 (MQ=255)

                                                                        acagccgtctcgagttcaacaatgacaaccgtaaactgcgcattaccgaagcggtatgccgtggtgccgttgaaactCTTCTGACGTTCAGACTCTGAACGCTAAAGTTGACCAGCTGAGCAACGACGTGAACGCAATGCGTTCCGACGt                                                                               <  1:4723836‑M2/73‑1 (MQ=255)

                                                                             cgtctcgagttcaacaatgacaaccgtaaactgcgcattaccgaagcggtatgccgtggtgccgttgaaactCTTCTGACGTTCAGACTCTGAACGCTAAAGTTGACCAGCTGAGCAACGACGTGAACGCAATGCGTTCCGACGTTCAgg                                                                          <  1:4059508‑M2/78‑1 (MQ=255)

                                                                                   gagttcaacaatgacaaccgtaaactgcgcattaccgaagcggtatgccgtggtgccgttgaaactCTTCTGACGTTCAGACTCTGAACGCTAAAGTTGACCAGCTGAGCAACGACGTGAACGCAATGCGTTCCGACGTTCAGGCTGCTa                                                                    <  1:2266092‑M2/84‑1 (MQ=255)

                                                                                     gttcaacaatgacaaccgtaaactgcgcattaccgaagcggtatgccgtggtgccgttgaaactCTTCTGACGTTCAGACTCTGAACGCTAAAGTTGACCAGCTGAGCAACGACGTGAACGCAATGCGTTCCGACGTTCAGGCTGCTaaa                                                                  >  2:1227716‑M2/65‑150 (MQ=255)

                                                                                      ttcaacaatgacaaccgtaaactgcgcattaccgaagcggtatgccgtggtgccgttgaaactCTTCTGACGTTCAGACTCTGAACGCTAAAGTTGACCAGCTGAGCAACGACGTGAACGCAATGCGTTCCGACGTTCAGGCTGCTAAAg                                                                 <  2:1951340‑M2/87‑1 (MQ=255)

                                                                                        caacaatgacaaccgtaaactgcgcattaccgaagcggtatgccgtggtgccgttgaaactCTTCTGACGTTCAGACTCTGAACGCTAAAGTTGACCAGCTGAGCAACGACGTGAACGCAATGCGTTCCGACGTTCAGGCTGCTAAAGAt                                                               >  1:3533271‑M2/62‑150 (MQ=255)

                                                                                        caacaatgacaaccgtaaactgcgcattaccgaagcggtatgccgtggtgccgttgaaactCTTCTGACGTTCAGACTCTGAACGCTAAAGTTGACCAGCTGAGCAACGACGTGAACGCAATGCGTTCCGACGTTCAGGCTGCTAAAGAt                                                               >  1:2216343‑M2/62‑150 (MQ=255)

                                                                                        caacaatgacaaccgtaaactgcgcattaccgaagcggtatgccgtggtgccgttgaaactCTTCTGACGTTCAGACTCTGAACGCTAAAGTTGACCAGCTGAGCAACGACGTGAACGCAATGCGTTCCGACGTTCAGGCTGCTAAAGAt                                                               >  2:2450935‑M2/62‑150 (MQ=255)

                                                                                           caatgacaaccgtaaactgcgcattaccgaagcggtatgccgtggtgccgttgaaactCTTCTGACGTTCAGACTCTGAACGCTAAAGTTGACCAGCTGAGCAACGACGTGAACGCAATGCGTTCCGACGTTCAGGCTGCTAAAGATGAc                                                            >  2:4331852‑M2/59‑150 (MQ=255)

                                                                                           caatgacaaccgtaaactgcgcattaccgaagcggtatgccgtggtgccgttgaaactCTTCTGACGTTCAGACTCTGAACGCTAAAGTTGACCAGCTGAGCAACGACGTGAACGCAATGCGTTCCGACGTTCAGGCTGCTAAAGATGAc                                                            <  1:572995‑M2/92‑1 (MQ=255)

                                                                                           caatgacaaccgtaaactgcgcattaccgaagcggtatgccgtggtgccgttgaaactCTTCTGACGTTCAGACTCTGAACGCTAAAGTTGACCAGCTGAGCAACGACGTGAACGCAATGCGTTCCGACGTTCAGGCTGCTAAAGATGAc                                                            >  1:4439885‑M2/59‑150 (MQ=255)

                                                                                             atgacaaccgtaaactgcgcattaccgaagcggtatgccgtggtgccgttgaaactCTTCTGACGTTCAGACTCTGAACGCTAAAGTTGACCAGCTGAGCAACGACGTGAACGCAATGCGTTCCGACGTTCAGGCTGCTAAAGATGACGc                                                          >  2:2293632‑M2/57‑150 (MQ=255)

                                                                                              tgacaaccgtaaactgcgcattaccgaagcggtatgccgtggtgccgttgaaactCTTCTGACGTTCAGACTCTGAACGCTAAAGTTGACCAGCTGAGCAACGACGTGAACGCAATGCGTTCCGACGTTCAGGCTGCTAAAGATGACGCa                                                         >  1:1661949‑M2/56‑150 (MQ=255)

                                                                                                   accgtaaactgcgcattaccgaagcggtatgccgtggtgccgttgaaactCTTCTGACGTTCAGACTCTGAACGCTAAAGTTGACCAGCTGAGCAACGACGTGAACGCAATGCGTTCCGACGTTCAGGCTGCTAAAGATGACGCAGCTCg                                                    >  1:3245084‑M2/51‑150 (MQ=255)

                                                                                                    ccgtaaactgcgcattaccgaagcggtatgccgtggtgccgttgaaactCTTCTGACGTTCAGACTCTGAACGCTAAAGTTGACCAGCTGAGCAACGACGTGAACGCAATGCGTTCCGACGTTCAGGCTGCTAAAGATGACGCAGCTCGt                                                   <  1:1718569‑M2/101‑1 (MQ=255)

                                                                                                    ccgtaaactgcgcattaccgaagcggtatgccgtggtgccgttgaaactCTTCTGACGTTCAGACTCTGAACGCTAAAGTTGACCAGCTGAGCAACGACGTGAACGCAATGCGTTCCGACGTTCAGGCTGCTAAAGATGACGCAGCTCGt                                                   <  2:4816479‑M2/101‑1 (MQ=255)

                                                                                                        aaactgcgcattaccgaagcggtatgccgtggtgccgttgaaactCTTCTGACGTTCAGACTCTGAACGCTAAAGTTGACCAGCTGAGCAACGACGTGAACGCAATGCGTTCCGACGTTCAGGCTGCTAAAGATGACGCAGCTCGTGCTa                                               >  1:4155994‑M2/46‑150 (MQ=255)

                                                                                                           ctgcgcattaccgaagcggtatgccgtggtgccgttgaaactCTTCTGACGTTCAGACTCTGAACGCTAAAGTTGACCAGCTGAGCAACGACGTGAACGCAATGCGTTCCGACGTTCAGGCTGCTAAAGATGACGCAGCTCGTGCTAAcc                                            >  2:290731‑M2/43‑150 (MQ=255)

                                                                                                             gcgcattaccgaagcggtatgccgtggtgccgttgaaactCTTCTGACGTTCAGACTCTGAACGCTAAAGTTGACCAGCTGAGCAACGACGTGAACGCAATGCGTTCCGACGTTCAGGCTGCTAAAGATGACGCAGCTCGTGCTAACCAg                                          <  1:1550669‑M2/110‑1 (MQ=255)

                                                                                                                cattaccgaagcggtatgccgtggtgccgttgaaactCTTCTGACGTTCAGACTCTGAACGCTAAAGTTGACCAGCTGAGCAACGACGTGAACGCAATGCGTTCCGACGTTCAGGCTGCTAAAGATGACGCAGCTCGTGCTAACCAGCGt                                       <  1:121860‑M2/113‑1 (MQ=255)

                                                                                                                   taccgaagcggtatgccgtggtgccgttgaaactCTTCTGACGTTCAGACTCTGAACGCTAAAGTTGACCAGCTGAGCAACGACGTGAACGCAATGCGTTCCGACGTTCAGGCTGCTAAAGATGACGCAGCTCGTGCTAACCAGCGTCTg                                    <  2:2163013‑M2/116‑1 (MQ=255)

                                                                                                                   taccgaagcggtatgccgtggtgccgttgaaactCTTCTGACGTTCAGACTCTGAACGCTAAAGTTGACCAGCTGAGCAACGACGTGAACGCAATGCGTTCCGACGTTCAGGCTGCTAAAGATGACGCAGCTCGTGCTAACCAGCGTCTg                                    <  1:1949053‑M2/116‑1 (MQ=255)

                                                                                                                     ccgaagcggtatgccgtggtgccgttgaaactCTTCTGACGTTCAGACTCTGAACGCTAAAGTTGACCAGCTGAGCAACGACGTGAACGCAATGCGTTCCGACGTTCAGGCTGCTAAAGATGACGCAGCTCGTGCTAACCAGCGTCTGGa                                  <  2:3074335‑M2/118‑1 (MQ=255)

                                                                                                                       gaagcggtatgccgtggtgccgttgaaactCTTCTGACGTTCAGACTCTGAACGCTAAAGTTGACCAGCTGAGCAACGACGTGAACGCAATGCGTTCCGACGTTCAGGCTGCTAAAGATGACGCAGCTCGTGCTAACCAGCGTCTGGaca                                >  2:2082073‑M2/31‑150 (MQ=255)

                                                                                                                         agcggtatgccgtggtgccgttgaaactCTTCTGACGTTCAGACTCTGAACGCTAAAGTTGACCAGCTGAGCAACGACGTGAACGCAATGCGTTCCGACGTTCAGGCTGCTAAAGATGACGCAGCTCGTGCTAACCAGCGTCTGGacaac                              >  2:4399590‑M2/29‑150 (MQ=255)

                                                                                                                          gcggtatgccgtggtgccgttgaaactCTTCTGACGTTCAGACTCTGAACGCTAAAGTTGACCAGCTGAGCAACGACGTGAACGCAATGCGTTCCGACGTTCAGGCTGCTAAAGATGACGCAGCTCGTGCTAACCAGCGTCTGGacaaca                             >  1:3332198‑M2/28‑150 (MQ=255)

                                                                                                                          gcggtatgccgtggtgccgttgaaactCTTCTGACGTTCAGACTCTGAACGCTAAAGTTGACCAGCTGAGCAACGACGTGAACGCAATGCGTTCCGACGTTCAGGCTGCTAAAGATGACGCAGCTCGTGCTAACCAGCGTCTGGacaaca                             <  2:1051331‑M2/123‑1 (MQ=255)

                                                                                                                            ggtatgccgtggtgccgttgaaactCTTCTGACGTTCAGACTCTGAACGCTAAAGTTGACCAGCTGAGCAACGACGTGAACGCAATGCGTTCCGACGTTCAGGCTGCTAAAGATGACGCAGCTCGTGCTAACCAGCGTCTGGACAACATg                           <  2:4869353‑M2/125‑1 (MQ=255)

                                                                                                                             gtatgccgtggtgccgttgaaactCTTCTGACGTTCAGACTCTGAACGCTAAAGTTGACCAGCTGAGCAACGACGTGAACGCAATGCGTTCCGACGTTCAGGCTGCTAAAGATGACGCAGCTCGTGCTAACCAGCGTCTGGACAACATgg                          >  2:4467092‑M2/25‑150 (MQ=255)

                                                                                                                              tatgccgtggtgccgttgaaactCTTCTGACGTTCAGACTCTGAACGCTAAAGTTGACCAGCTGAGCAACGACGTGAACGCAATGCGTTCCGACGTTCAGGCTGCTAAAGATGACGCAGCTCGTGCTAACCAGCGTCTGGACAACATGGc                         >  2:3349536‑M2/24‑150 (MQ=255)

                                                                                                                              tatgccgtggtgccgttgaaactCTTCTGACGTTCAGACTCTGAACGCTAAAGTTGACCAGCTGAGCAACGACGTGAACGCAATGCGTTCCGACGTTCAGGCTGCTAAAGATGACGCAGCTCGTGCTAA‑CAGCGTCTGGACAACCTGGct                        >  2:3105309‑M2/24‑150 (MQ=255)

                                                                                                                                  ccgtggtgccgttgaaactCTTCTGACGTTCAGACTCTGAACGCTAAAGTTGACCAGCTGAGCAACGACGTGAACGCAATGCGTTCCGACGTTCAGGCTGCTAAAGATGACGCAGCTCGTGCTAACCAGCGTCTGGACAACATGGctact                     <  2:1740313‑M2/131‑1 (MQ=255)

                                                                                                                                  ccgtggtgccgttgaaactCTTCTGACGTTCAGACTCTGAACGCTAAAGTTGACCAGCTGAGCAACGACGTGAACGCAATGCGTTCCGACGTTCAGGCTGCTAAAGATGACGCAGCTCGTGCTAACCAGCGTCTGGACAACATGGctact                     >  1:1256953‑M2/20‑150 (MQ=255)

                                                                                                                                     tggtgccgttgaaactCTTCTGACGTTCAGACTCTGAACGCTAAAGTTGACCAGCTGAGCAACGACGTGAACGCAATGCGTTCCGACGTTCAGGCTGCTAAAGATGACGCAGCTCGTGCTAACCAGCGTCTGGACAACATGGCTACTaaa                  >  2:185931‑M2/17‑150 (MQ=255)

                                                                                                                                      ggtgccgttgaaactCTTCTGACGTTCAGACTCTGAACGCTAAAGTTGACCAGCTGAGCAACGACGTGAACGCAATGCGTTCCGACGTTCAGGCTGCTAAAGATGACGCAGCTCGTGCTAACCAGCGTCTGGACAACATGGCTACTAAAt                 >  1:1446346‑M2/16‑150 (MQ=255)

                                                                                                                                        tgccgttgaaactCTTCTGACGTTCAGACTCTGAACGCTAAAGTTGACCAGCTGAGCAACGACGTGAACGCAATGCGTTCCGACGTTCAGGCTGCTAAAGATGACGCAGCTCGTGCTAACCAGCGTCTGGACAACATGGCTACTAAATAc               >  2:4061757‑M2/14‑150 (MQ=255)

                                                                                                                                        tgccgttgaaactCTTCTGACGTTCAGACTCTGAACGCTAAAGTTGACCAGCTGAGCAACGACGTGAACGCAATGCGTTCCGACGTTCAGGCTGCTAAAGATGACGCAGCTCGTGCTAACCAGCGTCTGGACAACATGGCTACTAAATAc               <  1:3567356‑M2/137‑1 (MQ=255)

                                                                                                                                         gccgttgaaactCTTCTGACGTTCAGACTCTGAACGCTAAAGTTGACCAGCTGAGCAACGACGTGAACGCAATGCGTTCCGACGTTCAGGCTGCTAAAGATGACGCAGCTCGTGCTAACCAGCGTCTGGACAACATGGCTACTAAATAcc              <  2:2708654‑M2/138‑1 (MQ=255)

                                                                                                                                            gttgaaactCTTCTGACGTTCAGACTCTGAACGCTAAAGTTGACCAGCTGAGCAACGACGTGAACGCAATGCGTTCCGACGTTCAGGCTGCTAAAGATGACGCAGCTCGTGCTAACCAGCGTCTGGACAACATGCCTACTAAATACCGCa           >  2:161690‑M2/10‑150 (MQ=255)

                                                                                                                                            gttgaaactCTTCTGACGTTCAGACTCTGAACGCTAAAGTTGACCAGCTGAGCAACGACGTGAACGCAATGCGTTCCGACGTTCAGGCTGCTAAAGATGACGCAGCTCGTGCTAACCAGCGTCTGGACAACACGGCTACTAAATACCGCa           <  2:678254‑M2/141‑1 (MQ=255)

                                                                                                                                                aaactCTTCTGACGTTCAGACTCTGAACGCTAAAGTTGACCAGCTGAGCAACGACGTGAACGCAATGCGTTCCGACGTTCAGGCTGCTAAAGATGACGCAGCTCGTGCTAACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTa       >  1:1840176‑M2/6‑150 (MQ=255)

                                                                                                                                                aaactCTTCTGACGTTCAGACTCTGAACGCTAAAGTTGACCAGCTGAGCAACGACGTGAACGCAATGCGTTCCGACGTTCAGGCTGCTAAAGATGACGCAGCTCGTGCTAACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTa       <  1:4623314‑M2/145‑1 (MQ=255)

                                                                                                                                                  actCTTCTGACGTTCAGACTCTGAACGCTAAAGTTGACCAGCTGAGCAACGACGTGAACGCAATGCGTTCCGACGTTCAGGCTGCTAAAGATGACGCAGCTCGTGCTAACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAAt     >  2:1143569‑M2/4‑150 (MQ=255)

                                                                                                                                                    tCTTCTGACGTTCAGACTCTGAACGCTAAAGTTGACCAGCTGAGCAACGACGTGAACGCAATGCGTTCCGACGTTCAGGCTGCTAAAGATGACGCAGCTCGTGCTAACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAATAg   >  2:3600566‑M2/2‑150 (MQ=255)

                                                                                                                                                    tCTTCTGACGTTCAGACTCTGAACGCTAAAGTTGACCAGCTGAGCAACGACGTGAACGCAATGCGTTCCGACGTTCAGGCTGCTAAAGATGACGCAGCTCGTGCTAACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAATAg   >  2:635802‑M2/2‑150 (MQ=255)

                                                                                                                                                     cttctGACGTTCAGACTCTGAACGCTAAAGTTGACCAGCTGAGCAACGACGTGAACGCAATGCGTTCCGACGTTCAGGCTGCTAAAGATGACGCAGCTCGTGCTAACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAATAGt  <  1:511006/150‑1 (MQ=255)

                                                                                                                                                     cttctGACGTTCAGACTCTGAACGCTAAAGTTGACCAGCTGAGCAACGACGTGAACGCAATGCGTTCCGACGTTCAGGCTGCTAAAGATGACGCAGCTCGTGCTAACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAATAGt  <  1:1528929/150‑1 (MQ=255)

CTTTGTGTAATACTTGTAACGCTACATGGAGATTAACTCAATCTAGAGGGTATTAATAATGAAAGCTACTAAACTGGTACTGGGCGCGGTAATCCTGGGTTCTACTCTGCTGGCAGGTTGCTCCAGCAACGCTAAAATCGATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAAAGTTGACCAGCTGAGCAACGACGTGAACGCAATGCGTTCCGACGTTCAGGCTGCTAAAGATGACGCAGCTCGTGCTAACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAATAGT  >  AP012306/1438946‑1439244

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`