Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123061,854,8510AC23.8% 750.5 / 60.4 298T25P (ACC→CCC) yejHpredicted ATP‑dependet helicase
Reads supporting (aligned to +/- strand):  ref base A (197/30);  new base C (0/71);  total (197/101)
Fisher's exact test for biased strand distribution p-value = 7.01e-45
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 340 total aligned reads displayed.

TAGCGGTCTAATAACATCCCTCAAGGGAAAAAGATCCATGGCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGAACTGGTGGCGCAAAACCATGCAAAGTATCAGGCGCTGGGGCTG  >  AP012306/1854702‑1855000
                                                                                                                                                     |                                                                                                                                                     
 aGCGGTCTAATAACATCCCTCAAGGGAAAAAGATCCATGGCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCGCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAAc                                                                                                                                                      >  1:4583957/1‑150 (MQ=255)
 aGCGGTCTAATAACATCCCTCAAGGGAAAAAGATCCATGGCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAAc                                                                                                                                                      <  1:1867392/150‑1 (MQ=255)
    ggTCTAATAACATCCCTCAAGGGAAAAAGATCCATGGCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAAcccc                                                                                                                                                   <  2:3218333/150‑1 (MQ=255)
     gTCTAATAACATCCCTCAAGGGAAAAAGATCCATGGCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCt                                                                                                                                                  <  1:211268/150‑1 (MQ=255)
       cTAATAACATCCCTCAAGGGAAAAAGATCCATGGCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGc                                                                                                                                                >  2:2590899/1‑150 (MQ=255)
       cTAATAACATCCCTCAAGGGAAAAAGATCCATGGCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGc                                                                                                                                                >  2:3113752/1‑150 (MQ=255)
          ataaCATCCCTCAAGGGAAAAAGATCCATGGCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGt                                                                                                                                             >  1:471959/1‑150 (MQ=255)
          ataaCATCCCTCAAGGGAAAAAGATCCATGGCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGt                                                                                                                                             <  2:1240341/150‑1 (MQ=255)
            aaCATCCCTCAAGGGAAAAAGATCCATGGCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTa                                                                                                                                           >  1:6306/1‑150 (MQ=255)
             aCATCCCTCAAGGGAAAAAGATCCATGGCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTAt                                                                                                                                          >  1:2631887/1‑150 (MQ=255)
             aCATCCCTCAAGGGAAAAAGATCCATGGCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTAt                                                                                                                                          >  1:2021706/1‑150 (MQ=255)
              cATCCCTCAAGGGAAAAAGATCCATGGCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATc                                                                                                                                         >  1:1461923/1‑150 (MQ=255)
              cATCCCCAAAGGGAAAAAGATCCATGGCATACTATTACCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCCCCCATATCAGCAAAAAGCCGTGGATCCCACGCTAAACCATTTTCGTCGTCATAAACCCCCTGCCGTTATc                                                                                                                                         <  2:4614522/150‑1 (MQ=255)
                tCCCTCAAGGGAAAAAGATCCATGGCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAACCCCCTGCCGTTATCGt                                                                                                                                       <  2:4037013/150‑1 (MQ=255)
                    tCAAGGGAAAAAGATCCATGGCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGtgctg                                                                                                                                   >  2:3683125/1‑150 (MQ=255)
                     cAAGGGAAAAAGATCCATGGAATACTATTAGCAAAATAATCTACCTAGGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGAGGATGTCACGCACAACCATATTCATCGTCTTAAAACCCCTGCCGTTATCGatctgc                                                                                                                                  >  2:4798741/1‑150 (MQ=255)
                      aaGGAAAAAAGATCCATGGCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAACCCCCTGCCGTTATCGTGCTGcc                                                                                                                                 <  1:3869871/150‑1 (MQ=255)
                        gggAAAAAGATCCATGGCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAACCCCCTGCCGTTATCGTGCTGCCCa                                                                                                                               <  1:355348/150‑1 (MQ=255)
                         ggAAAAAGATCCATGGCATACTATTAGCAGAATAATCTACCTACGCGAGACCTTGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCAc                                                                                                                              <  1:3367948/150‑1 (MQ=255)
                         ggAAAAAGATCCATGGCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCAc                                                                                                                              >  2:2546789/1‑150 (MQ=255)
                           aaaaaGATCCATGGCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCg                                                                                                                            >  2:1501935/1‑150 (MQ=255)
                            aaaaGATCCATGGCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCCCGCTCAACCATTTTCGTCGTCATAAACCCCCTGCCGTTATCGTGCTGCCCACCgg                                                                                                                           <  2:4819088/150‑1 (MQ=255)
                             aaaGATCCATGGCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGgc                                                                                                                          >  2:2483687/1‑150 (MQ=255)
                              aaGATCCATGGCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGgcg                                                                                                                         >  2:832108/1‑150 (MQ=255)
                               aGATCCATGGCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGgcgc                                                                                                                        >  1:2414131/1‑150 (MQ=255)
                                   ccATGGCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGt                                                                                                                    >  1:4626682/1‑150 (MQ=255)
                                   ccAGGGCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTGCGCCCATACCAGCAAGAGGCCGTGGATGCCCCGCTCAACCATTTGCGGCGTCATAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGt                                                                                                                    <  2:3007213/150‑1 (MQ=255)
                                     aTGGCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTaa                                                                                                                  >  2:637473/1‑150 (MQ=255)
                                       ggCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATGTCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTaaaa                                                                                                                >  1:3278381/1‑150 (MQ=255)
                                       ggCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTaaaa                                                                                                                >  2:4656394/1‑150 (MQ=255)
                                        gCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAg                                                                                                               >  2:4780296/1‑150 (MQ=255)
                                         cATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGc                                                                                                              <  1:1720117/150‑1 (MQ=255)
                                         cATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGc                                                                                                              >  2:2246853/1‑150 (MQ=255)
                                           tACAATTAGCAGAATAATCTACCTCCGCGAGACCATGATTTTTACCCTCCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTCCGTCGTCATAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCt                                                                                                            <  2:1700582/150‑1 (MQ=255)
                                            aCTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTg                                                                                                           >  1:1052914/1‑150 (MQ=255)
                                             cTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTgg                                                                                                          >  2:2801194/1‑150 (MQ=255)
                                              tATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGt                                                                                                         >  1:4255410/1‑150 (MQ=255)
                                               aTTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTg                                                                                                        >  1:4121865/1‑150 (MQ=255)
                                               aTTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTg                                                                                                        >  2:2445508/1‑150 (MQ=255)
                                               aTTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTg                                                                                                        >  1:2186485/1‑150 (MQ=255)
                                                 tAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGAt                                                                                                      >  1:4042711/1‑150 (MQ=255)
                                                   gCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAg                                                                                                    <  1:2432141/150‑1 (MQ=255)
                                                     aGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCg                                                                                                  >  1:4216652/1‑150 (MQ=255)
                                                     aGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCg                                                                                                  >  1:3715063/1‑150 (MQ=255)
                                                        ataatCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGaa                                                                                               >  2:1605786/1‑150 (MQ=255)
                                                          aatCTACCTCCGCGAGACCATGATTTTTACACTTCGCCCATATAAGCAAGAAGCCGGGGATGCCCCGCTCACCCTTTTTCGGCGTCAAAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACt                                                                                             <  2:1106391/150‑1 (MQ=255)
                                                              tacctacGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCa                                                                                         >  1:3428703/1‑150 (MQ=255)
                                                               ccctacGCGAGACCATGATTTTTACACTTCCCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGCCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCAc                                                                                        <  2:870612/149‑1 (MQ=255)
                                                                 ctccgcgAGACCATGATTTTTCCCCTCCCCCAATATCAGCAAGAAGCCGGGGATGCCCCGCTCAACCATTTTCGTCGTCAAAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACgg                                                                                      <  2:182886/147‑1 (MQ=255)
                                                                 ctacGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACgg                                                                                      <  1:3459114/150‑1 (MQ=255)
                                                                 ctacGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACgg                                                                                      <  2:1461923/150‑1 (MQ=255)
                                                                 ctacGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACgg                                                                                      >  1:3082509/1‑150 (MQ=255)
                                                                   acGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACggct                                                                                    >  2:4916646/1‑150 (MQ=255)
                                                                    cgcgAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGGCGTCAAAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACggctg                                                                                   <  2:4744791/150‑1 (MQ=255)
                                                                      cgAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACggctggc                                                                                 >  1:2262310/1‑150 (MQ=255)
                                                                       gagaCCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACggctggct                                                                                >  2:1923911/1‑150 (MQ=255)
                                                                        agaCCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTc                                                                               <  1:966055/150‑1 (MQ=255)
                                                                         gaCCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGTTCg                                                                              >  2:2750607/1‑150 (MQ=255)
                                                                         gaCCATGATTTTTACACTTCGCCCATAACAGCAAGAAGCCGTGGATGCCACGCCCAACCATTTTCGTCGTCATAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCg                                                                              <  2:246063/150‑1 (MQ=255)
                                                                             aTGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGt                                                                          >  1:432895/1‑150 (MQ=255)
                                                                                aTTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTcgc                                                                       >  1:4488904/1‑150 (MQ=255)
                                                                                 tttttACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTcgcg                                                                      >  1:2941163/1‑150 (MQ=255)
                                                                                   tttACCCTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCgtg                                                                    <  1:3769371/150‑1 (MQ=255)
                                                                                   tttACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCgtg                                                                    >  1:1638615/1‑150 (MQ=255)
                                                                                    ttACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCgtgc                                                                   >  2:4585142/1‑150 (MQ=255)
                                                                                       cacTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCgtgctcg                                                                >  1:3649474/1‑148 (MQ=255)
                                                                                         cTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCgtgctggtg                                                              <  2:2918304/150‑1 (MQ=255)
                                                                                            cGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCgtgctggtgctg                                                           >  1:1242876/1‑150 (MQ=255)
                                                                                             gCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGtgctggtgctgg                                                          >  1:2388879/1‑150 (MQ=255)
                                                                                             gCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGtgctggtgctgg                                                          >  1:2483027/1‑150 (MQ=255)
                                                                                              cccATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGc                                                         <  1:4415974/150‑1 (MQ=255)
                                                                                                cATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGcac                                                       >  1:2672470/1‑150 (MQ=255)
                                                                                                 atatCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGcaca                                                      >  1:51743/1‑150 (MQ=255)
                                                                                                 atatCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGcaca                                                      >  2:3348429/1‑150 (MQ=255)
                                                                                                 atatCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTCGGTCGTGGTCGCGTGCTGGTGGTGGcaaa                                                      >  2:2657592/1‑148 (MQ=255)
                                                                                                   atCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACg                                                    >  1:1299031/1‑150 (MQ=255)
                                                                                                      aGCAAGAAGCGGGGGATGCCGCGTTCACCCATTTTCGTCGTCATAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCGCGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTa                                                 <  1:895879/150‑1 (MQ=255)
                                                                                                      aGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTa                                                 <  1:1534706/150‑1 (MQ=255)
                                                                                                      aGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTa                                                 >  2:2044860/1‑150 (MQ=255)
                                                                                                        cAAGAAGCGGTGGATGCCACGCCCAACATTTTCCGCCGTAATAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGATGGCACACGTTaaa                                               <  1:1009657/150‑1 (MQ=255)
                                                                                                        cAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTaaa                                               >  1:1310840/1‑150 (MQ=255)
                                                                                                          agaagCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGa                                             >  2:4237497/1‑150 (MQ=255)
                                                                                                           gaagCCGTGGATGCCACGCTCACCCATTTTCGACGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGaa                                            <  2:1296555/150‑1 (MQ=255)
                                                                                                             agCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGAACt                                          >  2:229514/1‑150 (MQ=255)
                                                                                                                   ggATGCCACGCTCACCCATTTTCGTCGTCATAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGAACTGGTGgc                                    <  1:2849169/150‑1 (MQ=255)
                                                                                                                   ggATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGAACTGGTGgc                                    >  1:7746/1‑150 (MQ=255)
                                                                                                                     aTGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGAACTGGTGgcgc                                  >  2:2314040/1‑150 (MQ=255)
                                                                                                                      tGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGAACTGGTGGCGCa                                 >  2:3932463/1‑150 (MQ=255)
                                                                                                                      tGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGAACTGGTGGCGCa                                 >  1:4805376/1‑150 (MQ=255)
                                                                                                                        ccACGCTCAACCATTTTCGTCGTAATAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGAACTGGTGGCGCaaa                               <  2:378865/150‑1 (MQ=255)
                                                                                                                            gCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGGGGCCGCGGGCTGGTGCTGGCACACGTTAAAGAACTGGTGGCGCAAAACCa                           >  1:298538/1‑150 (MQ=255)
                                                                                                                             cTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGAACTGGTGGCGCAAAACCAt                          >  2:4359606/1‑150 (MQ=255)
                                                                                                                                  ccATTTTCGTCGTCATAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGAACTGGTGGCGCAAAACCATGCaaa                     <  1:3938188/150‑1 (MQ=255)
                                                                                                                                   cATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGAACTGGTGGCGCAAAACCATGCAAAg                    >  2:3753026/1‑150 (MQ=255)
                                                                                                                                     ttttCGTCGTCATAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGAACTGGTGGCGCAAAACCATGCAAAGTa                  <  2:4505965/150‑1 (MQ=255)
                                                                                                                                         cgtcgtCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGAACTGGTGGCGCAAAACCATGCAAAGTATCAg              >  2:1199278/1‑150 (MQ=255)
                                                                                                                                          gtcgtcAAAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGAACTGGTGGCGCAAAACCATGCAAAGTATCAgg             <  2:3429802/150‑1 (MQ=255)
                                                                                                                                                 aaaacCCCCTGCCCGTATCGGGCTGGCCACCGGCGGAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCCCGTGCTGGGGCTGGCACCCGTTAAAGAACTGGGTGCGCAAAACCATGCAAAGTACCAGGCGCTgga      <  2:2171298/149‑2 (MQ=255)
                                                                                                                                                  aaatCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGAACTGGTGGCGCAAAACCATGCAAAGTATCAGGCGCTgggg     <  2:3456048/150‑1 (MQ=255)
                                                                                                                                                   aaaCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGAACTGGTGGCGCAAAACCATGCAAAGTATCAGGCGCTGGGGc    >  2:724563/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TAGCGGTCTAATAACATCCCTCAAGGGAAAAAGATCCATGGCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGAACTGGTGGCGCAAAACCATGCAAAGTATCAGGCGCTGGGGCTG  >  AP012306/1854702‑1855000

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑