Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123062,558,0710AC25.8% 727.1 / 44.4 306V302G (GTA→GGA) visCpredicted oxidoreductase, FAD/NAD(P)‑binding domain
Reads supporting (aligned to +/- strand):  ref base A (153/74);  new base C (0/79);  total (153/153)
Fisher's exact test for biased strand distribution p-value = 3.28e-30
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 356 total aligned reads displayed.

CCTGCATACCAGCCAGCATCAACGCCGCACTGTGCTTGCGGCTACGCTCATAGCGACGCAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATC‑AGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAGTGGGAACACCTGACGCGCGCTCTCAACCTTGCATAAGCCCAGGCGATTATCAAAA  >  AP012306/2557922‑2558220
                                                                                                                                                      |                                                                                                                                                     
ccTGCATACCAGCCAGCATCAACGCCGCACTGTGCTTGCGGCTACGCTCATAGCGACGCAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATC‑AGCTCTGCAGCATCCATAAAGCCGAGATTTa                                                                                                                                                       <  2:4419143/150‑1 (MQ=255)
ccTGCATACCAGCCAGCATCAACGCCGCACTGTGCTTGCGGCTACGCTCATAGCGACGCAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATC‑AGCTCTGCAGCATCCATAAAGCCGAGATTTa                                                                                                                                                       <  1:4856407/150‑1 (MQ=255)
     aTACCAGCCAGCATCAACGCCGCACTGTGCTTGCGGCTACGCTCATAGCGACGCAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATC‑AGCTCTGCAGCATCCATAAAGCCGAGATTTAccccc                                                                                                                                                  <  1:3327270/150‑1 (MQ=255)
     aTACCAGCCAGCATCAACGCCGCACTGTGCTTGCGGCTACGCTCATAGCGACGCAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATC‑AGCTCTGCAGCATCCATAAAGCCGAGATTTAccccc                                                                                                                                                  <  1:3854945/150‑1 (MQ=255)
     aTACCAGCCAGCATCAACGCCGCACTGTGCTTGCGGCTACGCTCATAGCGACGCAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATC‑AGCTCTGCAGCATCCATAAAGCCGAGATTTAccccc                                                                                                                                                  <  1:2129454/150‑1 (MQ=255)
       aCCAGCCAGCATCAACGCCGCACTGTGCTTGCGGCTACGCTCATAGCGACGCAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATC‑AGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTg                                                                                                                                                <  1:4048001/150‑1 (MQ=255)
        ccagccagCATCAACGCCGCACTGTGCTTGCGGCTACGCTCATAGCGACGCAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATC‑AGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGc                                                                                                                                               >  2:2367011/1‑150 (MQ=255)
          agccagcATCAACGCCGCACTGTGCTTGCGGCTACGCTCATAGCGACGCAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATC‑AGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGccc                                                                                                                                             >  2:3730809/1‑150 (MQ=255)
              agcATCAACGCCGCACTGTGCTTGCGGCTACGCTCATAGCGACGCAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATC‑AGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGcc                                                                                                                                         >  2:32929/1‑150 (MQ=255)
                cATCAACGCCGCACTGTGCTTGCGGCTACGCTCATAGCGACGCAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATC‑AGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAg                                                                                                                                       >  1:3892932/1‑150 (MQ=255)
                 aTCAACGCCGCACTGTGCTTGCGGCTACGCTCATAGCGACGCAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATC‑AGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAGc                                                                                                                                      >  1:4677586/1‑150 (MQ=255)
                 aTCAACGCCGCACTGTGCTTGCGGCTACGCTCATAGCGACGCAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATC‑AGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAGc                                                                                                                                      >  1:3540958/1‑150 (MQ=255)
                 aTCAACGCCGCACTGTGCTTGCGGCTACGCTCATAGCGACGCAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATC‑AGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAGc                                                                                                                                      >  1:2535276/1‑150 (MQ=255)
                 aTCAACGCCGCACTGTGCTTGCGGCTACGCTCATAGCGACGCAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATC‑AGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAGc                                                                                                                                      >  2:2395987/1‑150 (MQ=255)
                   cAACGCCGCACTGTGCTTGCGGCTACGCTCATAGCGACGCAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATC‑AGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAGCgg                                                                                                                                    >  2:2342653/1‑150 (MQ=255)
                    accgccccCCTGTGCTGGCGGCTCCCCTCATCGCGACGCAAATAAATGTCCTCCCCGATGTCTTCCCCCCCGCGTTGAACCCGTTTCA‑CTCGCAAAGCAAGCACCGCGGAACCCATAAAGCCCAGATTTACCCCCTGCCCCGCCAGCggg                                                                                                                                   <  2:3158086/148‑1 (MQ=255)
                     aCGCCGCACTGTGCTTGCGGCTACGCTCATAGCGACGCAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATC‑AGCTCTGCAGCATCCATAAAGCCGAGATTTCCCCCCTGCCCCGCCAGCGGGt                                                                                                                                  <  2:1069804/150‑1 (MQ=255)
                       gccgcACTGTGCTTGCGGCTACGCTCATAGCGACGCAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATC‑AGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAGCGGGTGa                                                                                                                                >  1:1986050/1‑150 (MQ=255)
                        ccgcACTGTGCTGGCGGCTACGCTCATAGGGACGCAGATAAATGTACTGCCGGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATC‑GGCTCTGCAGCATCCATAAAGCCGAGATTTCCCCCCTGCCCCGCCAGCGGGTGaa                                                                                                                               <  2:2128790/150‑1 (MQ=255)
                          gcACTGTGCTTGCGGCTACGCTCATAGCGACGCAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATC‑AGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAGCGGGTGAATg                                                                                                                             >  2:647892/1‑150 (MQ=255)
                             cTGTGCTTGCGGCTACGCTCATAGCGACGCAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATC‑AGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAGCGGGTGAATGGTa                                                                                                                          >  1:1169945/1‑150 (MQ=255)
                               gtgCTTGCGGCTACGCTCATAGCGACGCAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATC‑AGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAGCGGGTGAATGGTAtg                                                                                                                        >  2:3067284/1‑150 (MQ=255)
                                  cTTGCGGCTACGCTCATAGCGACGCAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATC‑AGCTCTGCAGCATCCATAAAGCCGAGATTTCCCCCCTGCCCCGCCAGCGGGTGAATGGTATGTgc                                                                                                                     <  1:813484/150‑1 (MQ=255)
                                  cTTGCGGCTACGCTCATAGCGACGCAGAAAAATGTCCTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATC‑AGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAGCGGGTGAATGGTATGTgc                                                                                                                     <  2:2663997/150‑1 (MQ=255)
                                  cTTGCGGCTACGCTCATAGCGACGAAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCACCCGTTTCAGTTCGGCAATC‑AGCTCTGCAGCATCCATAAAGCCGAGATTTCCCCCCTGCCCCGCCAGCGGGTGAATGGTATGTgc                                                                                                                     <  2:380040/150‑1 (MQ=255)
                                   ttGCGGCTACGCTCATAGCGACGCAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATC‑AGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAGAGGGTGAATGGTATGTgcg                                                                                                                    >  2:4278073/1‑150 (MQ=255)
                                      cGGCTACGCTCATAGCGACGCAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATC‑AGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAGCGGGTGAATGGTATGTgcggcg                                                                                                                 >  2:1976728/1‑150 (MQ=255)
                                       ggCTACGCTCATAGCGACGCAGATAATTGTACTGCCCGATGTCTTTCCCCTGACGATGCACCCGTTTCAGTTCGGCAATC‑AGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGt                                                                                                                <  1:218176/150‑1 (MQ=255)
                                        gCTACGCTCATAGCGACGCAGATAAATGTACTGCCCGATGTCTTCCCCCTGACGATGCAACCGTTTCAGTTCGGCAATC‑AGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTc                                                                                                               >  2:1128526/1‑150 (MQ=255)
                                          tACGCTAATAGCGACGCAGATAAATGTACTGCCCGATGTCTTCCCCCTGACGATGCAACCGTTTCAGTTCGGCAACC‑AGCTCTGCAGCATCCATAAAGCCGAGATTTCCCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGc                                                                                                             <  2:3363006/150‑1 (MQ=255)
                                            cGCTAATAGCGACGCAGATAAATTTACTGCCCGATGTCTTTCCCCTGACGATGCACCCGTTTCAGTTCGGCAACC‑ACCTCGGCAGCATCCAAAAAGCCGAGATTTCCCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGccc                                                                                                           <  2:90702/150‑1 (MQ=255)
                                               tCATAGCGACGCAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATC‑AGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCccacc                                                                                                        >  1:2961348/1‑150 (MQ=255)
                                                   aGCGACGCAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATC‑AGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAgcg                                                                                                    >  1:4201155/1‑150 (MQ=255)
                                                       aCGCAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATC‑AGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAg                                                                                                >  1:2622138/1‑150 (MQ=255)
                                                         gCAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATC‑AGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGAc                                                                                              >  1:4709501/1‑150 (MQ=255)
                                                           aGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATC‑AGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACgg                                                                                            >  1:3400969/1‑150 (MQ=255)
                                                                      cTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATC‑AGCTCTGCAGCATCCATAAAGCCGAGATTTCCCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCaaa                                                                                 <  1:906069/150‑1 (MQ=255)
                                                                       tGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATC‑AGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAAc                                                                                <  1:1385467/150‑1 (MQ=255)
                                                                       tGCCCGATGTCTTCCCCCTGACGATGCAACCGTTTCAGTTCGGCAATC‑AGCTCTGCAGCATCCATAAAGCCGAGATTTCCCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAAc                                                                                <  2:1374272/150‑1 (MQ=255)
                                                                        gCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATC‑AGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACt                                                                               <  1:3173338/150‑1 (MQ=255)
                                                                             aTGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATC‑AGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGgcgc                                                                          >  1:1061849/1‑150 (MQ=255)
                                                                              tGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATC‑AGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGgcgcg                                                                         >  2:404735/1‑150 (MQ=255)
                                                                               gTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATC‑AGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGgcgcgc                                                                        >  2:2789069/1‑150 (MQ=255)
                                                                               gTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATC‑AGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGgcgcgc                                                                        >  2:4213003/1‑150 (MQ=255)
                                                                                 cTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATC‑AGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCAt                                                                      <  1:979361/150‑1 (MQ=255)
                                                                                 cTTCCCCCTGACGATGCAACCGTTTCAGTTCGGCAATC‑AGCTCTGCAGCATCCATAAAGCCGAGATTTCCCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCAt                                                                      <  2:3437056/150‑1 (MQ=255)
                                                                                  ttcccccTGACGATGCAACCGTTTCAGTTCGGCAATC‑AGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATa                                                                     <  1:3062843/147‑1 (MQ=255)
                                                                                  ttcccccTGACGATGCAACCGTTTCAGTCCGGCAACC‑AGCTCTGCAGCATCCATAAAGCCGAGATTTCCCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATa                                                                     <  2:369534/147‑1 (MQ=255)
                                                                                   ttCCCCTGACGATGCAACCGTTTCAGTTCGGCAATC‑AGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATaa                                                                    >  2:146443/1‑150 (MQ=255)
                                                                                    tCCCCTGACGATGCAACCGTTTCAGTTCGGCAATC‑AGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAAc                                                                   >  2:1625925/1‑150 (MQ=255)
                                                                                     ccccTGACGATGCAACCGTTTCAGTTCGGCAATC‑AGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACg                                                                  >  2:1573267/1‑150 (MQ=255)
                                                                                      cccTGACGATGCAACCGTTTCAGTTCGGCAATC‑AGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGc                                                                 >  1:4412626/1‑150 (MQ=255)
                                                                                       ccTGACGATGCAACCGTTTCAGTTCGGCAATC‑AGCTCTGCAGCATCCATAAAGCCGAGATTTCCCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGcc                                                                <  2:3916970/150‑1 (MQ=255)
                                                                                        cTGACGATGCAACCGTTTCAGTTCGGCAATC‑AGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGccc                                                               >  2:836543/1‑150 (MQ=255)
                                                                                               tGCAACCGTTTCAGTTCGGCAATC‑AGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAGt                                                        <  1:1710107/150‑1 (MQ=255)
                                                                                                  aaCCGTTTCAGTTCGGCAATC‑AGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAGTggg                                                     >  1:4535102/1‑150 (MQ=255)
                                                                                                    ccGTTTCAGTTCGGCAATC‑AGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTTGCGCGCATAACGCCCCGTCAGTGGGaa                                                   >  2:387691/1‑150 (MQ=255)
                                                                                                    ccGTTTCAGTTCGGCAATC‑AGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAGTGGGaa                                                   <  1:4161975/150‑1 (MQ=255)
                                                                                                      gTTTCAGTTCGGCAATC‑AGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAGTGGGAaca                                                 >  1:1572178/1‑150 (MQ=255)
                                                                                                      gTTTCAGTTCGGCAATC‑AGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAGTGGGAaca                                                 >  1:1923800/1‑150 (MQ=255)
                                                                                                       tttCAGTCCGGAAATC‑AGCCCGCCCGCACCCATAAAGCCGAGATTTCCCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAGTGGGAacac                                                <  2:349801/150‑1 (MQ=255)
                                                                                                          cAGTTCGGCAATC‑AGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAGTGGGAACACCTg                                             >  1:1641580/1‑150 (MQ=255)
                                                                                                           aGTTCGGCAATC‑AGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAGTGGGAACACCTGa                                            >  2:123351/1‑150 (MQ=255)
                                                                                                            gTTCGGCAATC‑AGCTCTGCAGCATCCATAAAGCCGAGATTTCCCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAGTGGGAACACCTGAc                                           <  1:4036053/150‑1 (MQ=255)
                                                                                                            gTTCGGCAATC‑AGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAGTGGGAACACCTGAc                                           <  1:684153/150‑1 (MQ=255)
                                                                                                             ttCGGCAATC‑AGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAGTGGGAACACCTGAcg                                          <  1:2723161/150‑1 (MQ=255)
                                                                                                             ttCGGCAATC‑AGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAGTGGGAACACCTGAcg                                          <  1:590099/150‑1 (MQ=255)
                                                                                                               cGGCAATC‑AGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAGTGGGAACACCTGAcgcg                                        >  1:2489302/1‑150 (MQ=255)
                                                                                                                        aGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAGTGGGAACACCTGACGCGCGCTCTCa                                >  2:4813237/1‑150 (MQ=255)
                                                                                                                        aGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAGTGGGAACACCTGACGCGCGCTCTCa                                >  1:4638671/1‑150 (MQ=255)
                                                                                                                        aGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAGTGGGAACACCTGACGCGCGCTCTCa                                >  1:1136062/1‑150 (MQ=255)
                                                                                                                         gCTCTGCAGCATCAATAAAGCCGAGATTTACCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAGTGGGAACACCTGACGCGCGCTCTCaa                               <  1:3635193/150‑1 (MQ=255)
                                                                                                                          ctctGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAGTGGGAACACCTGACGCGCGCTCTCAAc                              >  1:4115319/1‑150 (MQ=255)
                                                                                                                            ctGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAGTGGGAACACCTGACGCGCGCTCTCAACCt                            <  1:1263935/150‑1 (MQ=255)
                                                                                                                                agcaTCCATAAAGCCGAGATTTCCCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAGTGGGAACACCTGACGCGCGCTCTCAACCTTGCa                        <  2:4537299/150‑1 (MQ=255)
                                                                                                                                agcaTCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAGTGGGAACACCTGACGCGCGCTCTCAACCTTGCa                        <  1:2051245/150‑1 (MQ=255)
                                                                                                                                   aTCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAGCGGGAACACCTGCCGCGCGCTCTCAACCTTGCATaa                     >  2:2904122/1‑150 (MQ=255)
                                                                                                                                     ccATAAAGCCGAGATTTACCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAGTGGGAACACCTGACGCGCGCTCTCAACCTTGCATAAGc                   >  1:651001/1‑150 (MQ=255)
                                                                                                                                      cATAAAGCCGAGATTTACCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAGTGGGAACACCTGACGCGCGCTCTCAACCTTGCATAAGcc                  >  1:1009680/1‑150 (MQ=255)
                                                                                                                                      aaTAAAGCCGAGATTTCCCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAGTGGGAACACCTGACGCGCGCTCTCAACCTTGCATAAGcc                  <  2:945486/149‑1 (MQ=255)
                                                                                                                                       aTAAAGCCGAGATTTCCCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAGTGGGAACACCTGACGCGCGCTCTCAACCTTGCATAAGccc                 <  2:324994/150‑1 (MQ=255)
                                                                                                                                         aaaTCCGAGATTTACCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAGTGGGAACACCTGACGCGCGCTCTCAACCTTGCATAAGCCCAg               >  1:1531083/1‑150 (MQ=255)
                                                                                                                                         aaaGCCGAGATTTACCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAGTGGGAACACCTGACGCGCGCTCTCAACCTTGCATAAGCCCAg               >  1:2437670/1‑150 (MQ=255)
                                                                                                                                          aacccGAGTTCTCCCCCCGACCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAGTGGGAACACCTGACGCGCGCTCTCAACCTTGCATAAGCCCAgg              <  2:4703635/147‑1 (MQ=255)
                                                                                                                                          aaGCCGAGATTTACCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAGTGGGAACACCTGACGCGCGCTCTCAACCTTGCATAAGCCCAgg              <  1:2899723/150‑1 (MQ=255)
                                                                                                                                           aGCCGAGATTTACCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAGTGGGAACACCTGACGCGCGCTCTCAACCTTGCATAAGCCCAGGc             >  1:609538/1‑150 (MQ=255)
                                                                                                                                           aGCCGAGATTTACCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAGTGGGAACACCTGACGCGCGCTCTCAACCTTGCATAAGCCCAGGc             <  1:1794415/150‑1 (MQ=255)
                                                                                                                                            gCCGAGATTTCCCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAGTGGGAACACCTGACGCGCGCTCTCAACCTTGCATAAGCCCAGGCg            <  2:2284063/150‑1 (MQ=255)
                                                                                                                                             ccGAGATTTACCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAGTGGGAACACCTGACGCGCGCTCTCAACCTTGCATAAGCCCAGGCGa           <  1:2281781/150‑1 (MQ=255)
                                                                                                                                             ccGAGATTTACCCCCTGCCCCGCCAGCGCGTGACTGGTATGTGCGCCGCCGCCCACCCCCCCCACACGCTGCGCGGCCACCTGGCGCGCTTAACCCCCCGTCCGCGCGACCCCCTGACGCTCCCTCCCAACCTTCCATAAGCCCCGCCGa           >  2:501286/1‑150 (MQ=255)
                                                                                                                                              cGAGATTTCCCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAGTGGGAACACCTGACGCGCGCTCTCAACCTTGCATAAGCCCAGGCGAt          <  2:4349288/150‑1 (MQ=255)
                                                                                                                                              cGAGATTTACCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCGTAACGCCACGTCAGTGGGAACACCTGACGCGCGCGCTCAACCTTGCATAAGGCCAGGCGAt          >  2:20412/1‑150 (MQ=255)
                                                                                                                                              cGAGATTTACCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAGTGGGAACACCTGACGCGCGCTCTCAACCTTGCATAAGCCCAGGCGAt          <  1:1877771/150‑1 (MQ=255)
                                                                                                                                                agaTTTCCCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAGTGGGAACACCTGACGCGCGCTCTCAACCTTGCATAAGCCCAGGCGATTa        <  2:1813420/150‑1 (MQ=255)
                                                                                                                                                agaTTTACCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAGTGGGAACACCTGACGCGCGCTCTCAACCTTGCATAAGCCCAGGCGATTa        <  1:3260771/150‑1 (MQ=255)
                                                                                                                                                 gaTTTCCCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAGTGGGAACACCTGACGCGCGCTCTCAACCTTGCATAAGCCCAGGCGATTAt       <  2:4486740/150‑1 (MQ=255)
                                                                                                                                                 gaTTTACCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAGTGGGAACACCTGACGCGCGCTCTCAACCTTGCATAAGCCCAGGCGATTAt       <  1:3154677/150‑1 (MQ=255)
                                                                                                                                                  aTTTACCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAGTGGGAACACCTGACGCGCGCTCTCAACCTTGCATAAGCCCAGGCGATTATc      >  1:3987459/1‑150 (MQ=255)
                                                                                                                                                     tACCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTTGCGCGCATAACGCCCCGTCAGTGGGAACACCTGACGCGCGCTCTCAACCTTGCATAAGCCCAGGCGATTATCaaa   >  1:4816546/1‑150 (MQ=255)
                                                                                                                                                     caCCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAGTGGGAACACCTGACGCGCGCTCTCAACCTTGCATAAGCCCAGGCGATTATCaaa   <  1:2390648/149‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                     
CCTGCATACCAGCCAGCATCAACGCCGCACTGTGCTTGCGGCTACGCTCATAGCGACGCAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATC‑AGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAGTGGGAACACCTGACGCGCGCTCTCAACCTTGCATAAGCCCAGGCGATTATCAAAA  >  AP012306/2557922‑2558220

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑