Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123062,758,9090TG25.6% 671.4 / 21.3 274G33G (GGT→GGG) agaDN‑acetylgalactosamine‑specific enzyme IID component of PTS
Reads supporting (aligned to +/- strand):  ref base T (54/150);  new base G (70/0);  total (124/150)
Fisher's exact test for biased strand distribution p-value = 2.36e-31
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 359 total aligned reads displayed.

AGCGCAACCGCAGCCCGTTGCCAGTAAAAATGAAGAAGAGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGTTTTACCTGGGCGATGTTGCCGATCCT‑GAAAAAGATTTATAAGGACGAC‑AAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTCGGATTCCTGATGGGGTTATTAATTTCGATGGAAGA  >  AP012306/2758761‑2759058
                                                                                                                                                    |                                                                                                                                                       
  cgcAACCGCAGCCCGTTGCCAGTAAAAATGAAGAAGAGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGtttt                                                                                                                                                      <  1:1389595/150‑1 (MQ=255)
  cgcAACCGCAGCCCGTTGCCAGTAAAAATGAAGAAGAGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGtttt                                                                                                                                                      <  2:1754076/150‑1 (MQ=255)
     aaCCGCAGCCCGTTGCCAGTAAAAATGAAGAAGAGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGTTTTAcc                                                                                                                                                   <  1:212655/150‑1 (MQ=255)
        cGCAGCCCGTTGCCAGTAAAAATGAAGAAGAGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGGTTTTCCCgg                                                                                                                                                >  2:3210914/1‑150 (MQ=255)
         gCAGCCCGTTGCCAGTAAAAATGAAGAAGAGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGGTTTACCTggg                                                                                                                                               >  1:3989427/1‑150 (MQ=255)
           aGCCCGTTGCCAGTAAAAATGAAGAAGAGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGTTTTACCTGGGCg                                                                                                                                             <  2:2861743/150‑1 (MQ=255)
            gCCCGTTGCCAGTAAAAATGAAGAAGAGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGTTTTACCTGGGCGa                                                                                                                                            >  1:1495808/1‑150 (MQ=255)
              ccGTTGCCAGTAAAAATGAAGAAGAGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGGGGGTTTATCTGGGGGATg                                                                                                                                          >  2:687987/1‑150 (MQ=255)
              ccGTTGCCAGTAAAAATGAAGAAGAGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGTTTTTTCTGGGCGCTg                                                                                                                                          >  2:4101443/1‑150 (MQ=255)
                gTTGCCAGTAAAAATGAAGAAGAGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGTTTTACCTGGGCGATGtt                                                                                                                                        <  2:4756786/150‑1 (MQ=255)
                 ttGCCAGTAAAAATGAAGAAGAGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGTTTTACCTGGGCGATGTTg                                                                                                                                       <  1:4507816/150‑1 (MQ=255)
                 ttGCCAGTAAAAATGAAGAAGAGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGTTTTACCTGGGCGATGTTg                                                                                                                                       <  1:1591965/150‑1 (MQ=255)
                 gtGCCAGTAAAAATGAAGAAGAGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGTTTTACCTGGGCGATGTTg                                                                                                                                       <  2:3999753/149‑1 (MQ=255)
                    ccAGTAAAAATGAAGAAGAGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGTTTTACCTGGGCGATGTTGCCg                                                                                                                                    <  2:2393593/150‑1 (MQ=255)
                      aGTAAAAATGAAGAAGAGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGTTTTACCTGGGCGATGTTGCCGAt                                                                                                                                  >  1:2776616/1‑150 (MQ=255)
                      aGTAAAAATGAAGAAGAGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGGTTTACCTGGGCGATGTTGCCGAt                                                                                                                                  >  1:3560531/1‑150 (MQ=255)
                        tAAAAATGAAGAAGAGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGGTTTTCCTGGGCGGTGTTGCCGATcc                                                                                                                                >  1:1065236/1‑150 (MQ=255)
                        tAAAAATGAAGAAGAGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGGTTTTACTGGGCGAGGTTTCCGATcc                                                                                                                                >  2:1252404/1‑150 (MQ=255)
                         aaaaaTGAAGAAGAGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCTAGCTTTAACTACGAAAGGATGCAGGCGGGCGGTTTTACCTGGGCGATGTTGCCGATCCt                                                                                                                               <  2:3595831/150‑1 (MQ=255)
                           aaaTGAAGAAGAGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGTTTTACCTGGGCGATGTTGCCGATCCT‑Ga                                                                                                                            >  1:4322817/1‑150 (MQ=255)
                            aaTGAAGAAGAGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGTTTTACCTGGGCGATGTTGCCGATCCT‑Gaa                                                                                                                           <  1:3899607/150‑1 (MQ=255)
                            aaTGAAGAAGAGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGGTTTACCTGGGCGATGTTGCCGATCCT‑Gaa                                                                                                                           >  2:4424154/1‑150 (MQ=255)
                             aTGAAGAAGAGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGTTTTACCTGGGCGATGTTGCCGATCCT‑Gaaa                                                                                                                          <  2:4175482/150‑1 (MQ=255)
                              tGAAGAAGAGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGTTTTACCTGGGCGATGTTGCCGATCCT‑Gaaaa                                                                                                                         <  1:4057494/150‑1 (MQ=255)
                              tGAAGAAGAGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGTGTTAACTGGGGGATGTTTCCGATCCT‑Gaaaa                                                                                                                         >  1:4184433/1‑150 (MQ=255)
                                aagaagAGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGGTTTTCCTTGGCGGTGTTGCCGGTCCT‑GAAAAAg                                                                                                                       >  2:4786714/1‑150 (MQ=255)
                                aagaagAGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGGTTTACCTGGGCCGTGATTCCGAGCCT‑Gaaaaaa                                                                                                                       >  2:3254646/1‑149 (MQ=255)
                                  gaagaGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGTTTTTCCTGGGCGATGTTGCCGATCCT‑GAAAAAAAt                                                                                                                     >  2:3867355/1‑150 (MQ=255)
                                   aagaGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGTTTTACCTGGGCGATGTTGCCGATCCT‑GAAAAAGAtt                                                                                                                    <  2:1963793/150‑1 (MQ=255)
                                    agagGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGTTTTTCCTTGGCGATGTTTGCGATCCT‑GGAAAAAAttt                                                                                                                   >  1:939522/1‑150 (MQ=255)
                                     gagGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGGTTTACCTGGGCGATGTTGCCGATCCT‑GAAAAAGATTta                                                                                                                  >  1:3373453/1‑150 (MQ=255)
                                      agGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGTTTTACCTGGGCGATGTTGCCGATCCT‑GAAAAAGATTtat                                                                                                                 >  1:3428323/1‑150 (MQ=255)
                                      agGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGTTTTACCTGGGCGATGTTGCCGATCCT‑GAAAAAGATTtat                                                                                                                 <  1:980935/150‑1 (MQ=255)
                                      agGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGGTTTTCCTGGGCGATGTTTGCGATCCT‑GAAAAAGATTtat                                                                                                                 >  2:3787593/1‑150 (MQ=255)
                                       ggACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGTTTTACCTGGGCGATGTTGCCGATCCT‑GAAAAAGATTtata                                                                                                                <  1:1527460/150‑1 (MQ=255)
                                       ggACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCACTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGGTTTTCCTCGGCCATGCTGTCCCTCCC‑GAAAAAGATTtata                                                                                                                >  1:2065251/1‑150 (MQ=255)
                                            aCAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCGGGCGGGCGGTTTTACCTGGGCGATGTTGCCGATCCT‑GAAAAAGATTTATAAGgac                                                                                                           >  1:4584583/1‑150 (MQ=255)
                                            aCAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGTTTTTCCTTGGCGGTGGTTCCGGACCTGGAAAAAGGttttaaagac                                                                                                            >  2:1147673/1‑143 (MQ=255)
                                            aCAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGTTTTACCTGGGCGATGTTGCCGATCCT‑GAAAAAGATTTATAAGgac                                                                                                           <  2:3525113/150‑1 (MQ=255)
                                             cAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGGTTTTCCTGGGCGATGGTGCCGGTCCT‑GAAAAAAATTTATAAGgacg                                                                                                          >  2:541351/1‑150 (MQ=255)
                                             cAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGCTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGTGGTTTTTCCCTTGCGAAGTTTCCGATCCT‑GTATAAGGTTTATAAGgact                                                                                                          >  1:3238125/1‑149 (MQ=255)
                                              aGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGGTTTACCTGGGCGATGTTGCCGATCCT‑GAAAAAGATTTATAAAgacga                                                                                                         >  1:2744317/1‑150 (MQ=255)
                                                cAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGTTTTACCTGGGCGATGTTGCCGATCCT‑GAAAAAGATTTATAAGGACGAC‑a                                                                                                      <  1:1668592/150‑1 (MQ=255)
                                                cAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGTTTTACCTGGGCGATGTTGCCGATCCT‑GAAAAAGATTTATAAGGACGAC‑a                                                                                                      <  1:1766684/150‑1 (MQ=255)
                                                cAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGGTTTTCCTGGGCGATGTTGCCGATCCT‑GAAAAAGATTTTTTAAGACGAC‑a                                                                                                      >  2:2474826/1‑150 (MQ=255)
                                                 aaTGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGGTTTTACTGGGGGGTGTTGGCGGGACT‑GAAAAAGAATTATAAGGACGGC‑aa                                                                                                     >  2:589369/1‑150 (MQ=255)
                                                  aTGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGTCGGGTTTTCCTTGGCCGAGTTGCCCATACT‑GTAAAAAATTTTTAAAGACGAC‑aaa                                                                                                    >  2:2062250/1‑150 (MQ=255)
                                                  aTGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGTTTTACCTGGGCGATGTTGCCGATCCT‑GAAAAAGATTTATAAGGACGAC‑aaa                                                                                                    <  1:2271439/150‑1 (MQ=255)
                                                   tGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGTTTTACCTGGGCGATGTTGCCGATCCT‑GAAAAAGATTTATAAGGACGAC‑AAAc                                                                                                   <  2:3041455/150‑1 (MQ=255)
                                                    gggATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGTTTTACCTGGGCGATGTTGCCGATCCT‑GAAAAAGATTTATAAGGACGAC‑AAAcc                                                                                                  <  1:478147/150‑1 (MQ=255)
                                                    gggATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGTTTTACCTGGGCGATGTTGCCGATCCT‑GAAAAAGATTTATAAGGACGAC‑AAAcc                                                                                                  >  2:3744556/1‑150 (MQ=255)
                                                      gATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCACTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGAGGGTTTACCTGGGCGGAGTTTCCGATCCC‑TGAAAAGAGTTTTAAAGGCGCC‑aaaaccg                                                                                                >  2:4602266/1‑146 (MQ=255)
                                                       aTCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGTTTTACCTGGGCGATGTTGCCGATCCT‑GAAAAAGATTTATAAGGACGAC‑AAACCggg                                                                                               >  2:4372201/1‑150 (MQ=255)
                                                       aTCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGTTTTACCTGGGCGATGTTGCCGATCCT‑GAAAAAGATTTATAAAGACGAC‑AAACCcgg                                                                                               >  1:2899211/1‑150 (MQ=255)
                                                         cTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGGTTTTTCTTGGGGGTGTTTCCGATCCC‑GAAAAAAAATTTTAAAGGCGACAAAACCgggt                                                                                              >  2:3645900/1‑149 (MQ=255)
                                                          tGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGTTTTACCTGGGCGATGTTGCCGATCCT‑GAAAAAGATTTATAAGGACGAC‑AAACCGGGCtt                                                                                            <  1:4241569/150‑1 (MQ=255)
                                                          tGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGGTTTAACTCGGCCAGGGTGGCGATTCT‑GCAAAAGATTTATAAAGACGGA‑AAACCCGGcct                                                                                            >  1:889834/1‑148 (MQ=255)
                                                           gAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGTTTTACCTGGGGGATGTTGCCGGTCCT‑GAAAAAGATTTATAAGGACGAC‑AAACCGGGCTTa                                                                                           >  2:1468255/1‑150 (MQ=255)
                                                            aaaTCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGTTTTACCTGGGCGATGTTGCCGATCCT‑GAAAAAGATTTATAAGGACGAC‑AAACCGGGCTTaa                                                                                          <  1:1295090/150‑1 (MQ=255)
                                                             aaTCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGTTTTACCTGGGCGATGTTGCCGATCCT‑GAAAAAGATTTATAAGGACGAC‑AAACCGGGCTTAAg                                                                                         <  2:1687688/150‑1 (MQ=255)
                                                                  gTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGTTTTACCTGGGCGATGTTGCCGATCCT‑GAAAAAGATTTATAAGGACGAC‑AAACCGGGCTTAAGCgcgg                                                                                    <  1:2464319/150‑1 (MQ=255)
                                                                  gTAAAAAAGATAGCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGTTTTACCTGGGCGATGTTGCCGATCCT‑GAAAAAGATTTATAAGGACGAC‑AAACCGGGCTTAAGCgcgg                                                                                    <  1:1917795/150‑1 (MQ=255)
                                                                    aaaaaaGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGTTTTACCTGGGCGATGTTGCCGATCCT‑GAAAAAGATTTATAAGGACGAC‑AAACCGGGCTTAAGCgcggcg                                                                                  <  2:4474576/150‑1 (MQ=255)
                                                                     aaaaaGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGGTTTACCTGGGCGATGTTGCCGATCCT‑GAAAAAGATTTATAAGGACGAC‑AAACCGGGCTTAAGCGCGGCGa                                                                                 >  1:4672616/1‑150 (MQ=255)
                                                                       aaaGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGTTTTACCTGGGCGATGTTTCCGATCCT‑TTAAAAGATTTATAAAGGAGAC‑AAACCCGGCTTAAGCGCGGCGATg                                                                               >  2:2129195/1‑150 (MQ=255)
                                                                        aaGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGGTTTACCTGGGCGATGTTGCCGATCCT‑GAAAAAGATTTTTAAGGACGAC‑AAAACGGGCTTAAGCGCGGCGGTGa                                                                              >  2:3674284/1‑150 (MQ=255)
                                                                           atatCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGTTTTACCTGGGCGATGTTGCCGATCCT‑GAAAAAGATTTATAAGGACGAC‑AAACCGGGCTTAAGCGCGGCGATGAAAg                                                                           <  1:543102/150‑1 (MQ=255)
                                                                             atCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGTTTTACCTGGGCGATGTTGCCGATCCT‑GAAAAAGATTTATAAGGACGAC‑AAACCGGGCTTAAGCGCGGCGATGAAAGAt                                                                         <  1:4136084/150‑1 (MQ=255)
                                                                                 cccGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGGTTTACCTGGGCGATGTTGCCGATCCT‑GAAAAAGATTTATAAGGACGAC‑AAACCGGGCTTAAGCGCGGCGATGAAAGATAAcc                                                                     >  1:3938680/1‑150 (MQ=255)
                                                                                   cGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGGTTTTCCTGGGCGGTGTTGCCGGTCCT‑GAAAAAGATTTATAAGGACGAC‑AAACCGGGCTTAAGCGCGGCGGTGAAAGAGAACCTc                                                                   >  2:2190450/1‑150 (MQ=255)
                                                                                   cGTCTGGGCTTTCGTTCGACGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGGTTTACCTGGGCGATGTTGCCCAACCT‑GAAAAAGATTTATAAGGACGAC‑AAACCGGGCTTAAGCGCGGCGATGTAAGATAACCTc                                                                   >  2:4565229/1‑150 (MQ=255)
                                                                                     tCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGGTTTACCTGGGCGATGTTGCCGATCCT‑GAAAAAGATTTATAAGGACGAC‑AAACCGGGCTTAAGCGCGGCGATGAAAGAAAAACTcca                                                                 >  2:2844657/1‑148 (MQ=255)
                                                                                       tGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGTTTTACCTCGGCGATTTTGCCGATCCT‑GAAAAAAATTTATAAGGACGAC‑AAACCCGGCTTAAACGCGGCGATGAAAAATAACCTCCAAt                                                               >  2:1549952/1‑150 (MQ=255)
                                                                                       tGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGGTTTACCTGGGCGATGTTGCCGATCCT‑GAAAAAGATTTATAAGGACGAC‑AAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAAt                                                               >  1:3132405/1‑150 (MQ=255)
                                                                                        gggCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGTTTTACCTGGGCGATGTTGCCGATCCT‑GAAAAAGATTTATAAGGACGAC‑AAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAAtt                                                              <  1:2631409/150‑1 (MQ=255)
                                                                                          gCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGGTTTACCTGGGCGATGTTGCCGATCCT‑GAAAAAGATTTATAAGGACGAC‑AAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATtta                                                            >  1:1817447/1‑150 (MQ=255)
                                                                                           cTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGGTTTACCTGGGCGATGTTGCCGATCCT‑GAAAAAGATTTATAAGGACGAC‑AAACCGGGCTTAAGCGCGGCGATGGAAGATAAACTCGAATttat                                                           >  2:2735307/1‑150 (MQ=255)
                                                                                              tcgttcgtCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGTTTTACCTGGGCGATGTTGCCGATCCT‑GAAAAAGATTTATAAGGACGAC‑AAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTaa                                                        <  1:4700784/150‑1 (MQ=255)
                                                                                                    gtcgCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGGTTTTCCTGGGCGATGTTGCCGATCCT‑GAAAAAAATTTATAAAGACGAC‑AAACCGGGCTTAAACGCGGCGATGAAAAATAAACTCGAATTTATTAATaacca                                                  >  2:2648182/1‑150 (MQ=255)
                                                                                                           ctgcAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGTTTTTCCTGGGGGATGTTGCCGATCCT‑GAAAAAGATTTATAAGGACGAC‑AAAACGGGGTTAAGCGCGGGGATGAAAGATAAACTCGAATTTATTAATACCCACCCGAAt                                           >  2:827262/1‑150 (MQ=255)
                                                                                                           ctgcAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGTTTTACCTGGGCGATGTTGCCGATCCT‑GAAAAAGATTTATAAGGACGAC‑AAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAAt                                           <  1:3792435/150‑1 (MQ=255)
                                                                                                             gcAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGTTTTACCTGGGCGATGTTGCCGATCCT‑GAAAAAGATTTATAAGGACGAC‑AAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATCCCCACCCGAATCt                                         <  2:1399065/150‑1 (MQ=255)
                                                                                                             gcAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGTTTTACCTGGGCGATGTTGCCGATCCT‑GAAAAAGATTTATAAGGACGAC‑AAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCt                                         >  1:2654738/1‑150 (MQ=255)
                                                                                                                    aGCTTTAACTACGAAAGGATGCAGGCGGGCGGGTTTTCCTGGGGGATGTTGCCCGTTCT‑GGAAAAGATTTATAAGGGCGAC‑AAACCGGGGTTTAGCGCGGGGATGAAAGATAAACTCGAATTTTTTAATAACCACCCGGATCTGGGCGGa                                  >  2:1030795/1‑150 (MQ=255)
                                                                                                                       tttAACTACGAAAGGATGCAGGCGGGCGGTTTTACCTGGGCGATGTTGCCGATCCT‑GAAAAAGATTTATAAGGACGAC‑AAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTCGGATTc                               <  2:918811/150‑1 (MQ=255)
                                                                                                                       tttAACTACGAAAGGATGCAGGCGGGCGGTTTTACCTGGGCGATGTTGCCGATCCT‑GAAAAAGATTTATAAGGACGAC‑AAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTCGGATTc                               <  2:2301966/150‑1 (MQ=255)
                                                                                                                        ttAACTACGAAAGGATGCAGGCGGGCGGTTTTACCTGGGCGATGTTGCCGATCCT‑GAAAAAGATTTATAAGGACGAC‑AAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTCGGATTcc                              <  1:2398726/150‑1 (MQ=255)
                                                                                                                          aaCTACGAAAGGATGCAGGCGGGCGGGTTTACCTGGGCGATGTTGCCGATCCT‑GAAAAAGATTTATAAGGACGAC‑AAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTCGGATTTCTg                            >  2:3930961/1‑150 (MQ=255)
                                                                                                                            cTACGAAAGGATGCAGGCGGGCGGTTTTTCCCGGGCGGTGTTGCCGATCCT‑GAAAAAGATTTTTAAGGACGAC‑AAAACCGGCTTAAGCGCGGCGATGAAAGATAAACTCGGATTTATTAATACCCACCCCAATCTGGGCGGATTCCTGAt                          >  2:2935423/1‑150 (MQ=255)
                                                                                                                            cTACGAAAGGATGCAGGCGGGCGGTTTTACCTGGGCGATGTTGCCGATCCT‑GAAAAAGATTTATAAGGACGAC‑AAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTCGGATTCCTGAt                          <  1:1844655/150‑1 (MQ=255)
                                                                                                                            cTACGAAAGGATGCAGGCGGGCGGTTTTACCTGGGCGATGTTGCCGATCCT‑GAAAAAGATTTATAAGGACGAC‑AAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTCGGATTCCTGAt                          <  1:4863987/150‑1 (MQ=255)
                                                                                                                                         cAGGCGGGCGGTTTTACCTGGGCGATGTTGCCGATCCT‑GAAAAAGATTTATAAGGACGAC‑AAACCGGGCTTAAGCGCGGCGGTGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTCGGATTCCTGATGGGGTTATTAAtt             >  2:2251106/1‑150 (MQ=255)
                                                                                                                                          aGGCGGGCGGTTTTACCTGGGCGATGTTGCCGATCCT‑GAAAAAGATTTATAAGGACGAC‑AAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTCGGATTCCTGATGGGGTTATTAAttt            >  2:1997544/1‑150 (MQ=255)
                                                                                                                                               ggcggTTTTACCTGGGCGATGTTGCCGATCCT‑GAAAAAGATTTATAAGGACGAC‑AAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTCGGATTCCTGATGGGGTTATTAATTTCGATg       <  1:3320226/150‑1 (MQ=255)
                                                                                                                                                gcggTTTTACCTGGGCGATGTTGCCGATCCT‑GAAAAAGATTTATAAGGACGAC‑AAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTCGGATTCCTGATGGGGTTATTAATTTCGATgg      <  1:1160735/150‑1 (MQ=255)
                                                                                                                                                gcggTTTTACCTGGGCGATGTTGCCGATCCT‑GAAAAAGATTTATAAGGACGAC‑AAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTCGGATTCCTGATGGGGTTATTAATTTCGATgg      <  1:695594/150‑1 (MQ=255)
                                                                                                                                                 cggTTTTACCTGGGCGATGTTGCCGATCCT‑GAAAAAGATTTATAAGGACGAC‑AAACCGGGGTTAAGCGCGGCGATGGAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTCGGATTCCTGATGGGGTTATTAATTTCGATGga     <  1:3666129/150‑1 (MQ=255)
                                                                                                                                                  ggTTTTACCTGGGCGATGTTGCCGATCCT‑GAAAAAGATTTATAAGGACGAC‑AAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTCGGATTCCTGATGGGGTTATTAATTTCGATGgaa    >  1:2888151/1‑150 (MQ=255)
                                                                                                                                                   gTTTTACCTGGGCGATGTTGCCGATCCT‑GAAAAAGATTTATAAGGACGAC‑AAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTCGGATTCCTGATGGGGTTATTAATTTCGATGgaag   >  2:947047/1‑150 (MQ=255)
                                                                                                                                                    ttttACCTGGGCGATGTTGCCGATCCT‑GAAAAAGATTTATAAGGACGAC‑AAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTCGGATTCCTGATGGGGTTATTAATTTCGATGgaaga  >  2:3385954/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                       
AGCGCAACCGCAGCCCGTTGCCAGTAAAAATGAAGAAGAGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGTTTTACCTGGGCGATGTTGCCGATCCT‑GAAAAAGATTTATAAGGACGAC‑AAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTCGGATTCCTGATGGGGTTATTAATTTCGATGGAAGA  >  AP012306/2758761‑2759058

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑