Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123063,020,6700TG31.3% 334.5 / 21.8 198K195N (AAA→AAC) glgCglucose‑1‑phosphate adenylyltransferase
Reads supporting (aligned to +/- strand):  ref base T (107/29);  new base G (62/0);  total (169/29)
Fisher's exact test for biased strand distribution p-value = 1.32e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 305 total aligned reads displayed.

ATCAAATCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTC‑AACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGATAAGCATACGCGAGTAGTCTTGCTT  >  AP012306/3020521‑3020819
                                                                                                                                                     |                                                                                                                                                      
aTCAAATCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGt                                                                                                                                                        <  1:3800468/150‑1 (MQ=255)
  cAAATCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTGGCAGGgtt                                                                                                                                                      >  2:1045806/1‑150 (MQ=255)
  cAAATCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGttt                                                                                                                                                      <  1:1143041/150‑1 (MQ=255)
  cAAATCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGGTTGCAGGgtt                                                                                                                                                      >  2:3085166/1‑150 (MQ=255)
  cAAATCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGGTAGCAGGgtt                                                                                                                                                      >  2:3437623/1‑150 (MQ=255)
   aaaTCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGtttt                                                                                                                                                     <  2:2614676/150‑1 (MQ=255)
   aaaTCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGGTTGCAGGgttt                                                                                                                                                     >  2:2878557/1‑150 (MQ=255)
     aTCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGGTAGCAGGGTTTTc                                                                                                                                                   >  2:4200080/1‑150 (MQ=255)
      tCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTTGCCGGgttttta                                                                                                                                                  >  1:3554373/1‑148 (MQ=255)
       cTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTC‑aa                                                                                                                                                <  2:4766242/150‑1 (MQ=255)
           gCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTC‑AACGaa                                                                                                                                            <  2:4845873/150‑1 (MQ=255)
            ccAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGGTAGCAGGTTTTTT‑AACGAAt                                                                                                                                           >  2:68062/1‑150 (MQ=255)
            ccAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGGTAGCAGGGTTTTT‑CACGAAt                                                                                                                                           >  2:2835000/1‑150 (MQ=255)
             cAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTC‑AACGAAtt                                                                                                                                          <  1:2821854/150‑1 (MQ=255)
             cAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGGTTTTC‑AACGAAtt                                                                                                                                          >  1:4298760/1‑150 (MQ=255)
               aaGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTC‑AACGAATTCg                                                                                                                                        <  1:627245/150‑1 (MQ=255)
               aaGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTC‑AACGAATTCg                                                                                                                                        <  1:2270549/150‑1 (MQ=255)
               aaGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGGTTTTC‑AACGAATTCg                                                                                                                                        >  1:1808254/1‑150 (MQ=255)
                 gTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTT‑AACGAATTTGAt                                                                                                                                      >  1:1369312/1‑150 (MQ=255)
                    gTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTC‑AACGAATTCGATAAt                                                                                                                                   <  1:983991/150‑1 (MQ=255)
                    gTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGGTAGCAGGTTTTTC‑CACGAATTCGATAAt                                                                                                                                   >  1:3750671/1‑150 (MQ=255)
                     tGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGGTTTTC‑AACGAATTCGATAAtt                                                                                                                                  >  1:317048/1‑150 (MQ=255)
                      ggCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGGTTTTC‑AACGAATTCGATAAttt                                                                                                                                 >  2:4430533/1‑150 (MQ=255)
                      ggCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGCTTTTC‑AACGAATTCGATAAttt                                                                                                                                 >  2:4277572/1‑150 (MQ=255)
                      ggCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGGTAGCAGGTTTTTT‑AACGAATTTGATAAttt                                                                                                                                 >  1:918035/1‑150 (MQ=255)
                       gCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTC‑AACGAATTCGATAAtttt                                                                                                                                <  2:800862/150‑1 (MQ=255)
                       gCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTC‑AACGAATTCGATAAtttt                                                                                                                                >  1:2360653/1‑150 (MQ=255)
                        cTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGGTTTTC‑AACGAATTCGATAATTTTa                                                                                                                               >  1:858420/1‑150 (MQ=255)
                            aGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTC‑AACGAATTCGATAATTTTATCGt                                                                                                                           <  1:2074673/150‑1 (MQ=255)
                             gTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTC‑AACGAATTCGATAATTTTATCGtt                                                                                                                          <  2:1805849/150‑1 (MQ=255)
                             gTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGGTAGCAGGGTTTTC‑AACGAATTCGATAATTTTATCGtt                                                                                                                          >  2:4569489/1‑150 (MQ=255)
                             gTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGATTAGCAGGTTTTTT‑AAACAATTTGTTAAtttttttcat                                                                                                                          >  2:958589/1‑144 (MQ=255)
                                ctcATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTC‑AACGAATTCGATAATTTTATCGTtttc                                                                                                                       >  2:3203639/1‑150 (MQ=255)
                                  cATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTC‑AACGAATTCGATAATTTTATCGTTCtcat                                                                                                                     >  1:257109/1‑150 (MQ=255)
                                   aTCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTC‑AACGAATTCGATAATTTTATCGTTCtcatc                                                                                                                    <  1:1522668/150‑1 (MQ=255)
                                        gATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTC‑AACGAATTCGATAATTTTATCGTTCTCATCAACCg                                                                                                               >  1:1915402/1‑150 (MQ=255)
                                         aTCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTC‑AACGAATTCGATAATTTTATCGTTCTCATCAACCGc                                                                                                              <  1:756236/150‑1 (MQ=255)
                                          tCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGGTAGCAGGGTTTTC‑AACGAATTCGATAATTTTATCGTTCTCATCAACCGcc                                                                                                             >  2:665603/1‑150 (MQ=255)
                                           cGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTC‑AACGAATTCGATAATTTTATCGTTCTCATCAACCGCCa                                                                                                            >  1:3642091/1‑150 (MQ=255)
                                            gTCTTCTCCCAGCAGTTCATAAAGATAGTCGGCGTAAAAGACGAAGACCCCCATCCCCGCAAGAGTTTTGCTCGGATCGTTCGGCATTGACGGGGGTTTAGCGGTTTTTTC‑AACAAATTCGATAATTTTATCGTTCTCATCACCCGCCAt                                                                                                           <  2:3941195/150‑1 (MQ=255)
                                              cttcttCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTC‑AACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATaa                                                                                                         >  1:4163097/1‑150 (MQ=255)
                                              cttcttCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGGTAGCAGGTTTTTC‑AACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATaa                                                                                                         >  1:1434949/1‑150 (MQ=255)
                                               ttcttcCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTC‑AACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAAc                                                                                                        >  2:2188008/1‑150 (MQ=255)
                                                tcttcCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGGTAGCAGGGTTTTC‑AACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACg                                                                                                       >  2:3733861/1‑150 (MQ=255)
                                                 cttcCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTC‑AACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGc                                                                                                      >  1:3446518/1‑150 (MQ=255)
                                                     cagcagTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGGTAGCAGGTTTTTT‑AACGGATTCGGTAATTTTTTCGTTCTCATCAACCGCCATAACGCCaaa                                                                                                  >  2:2705498/1‑150 (MQ=255)
                                                         agTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGGTTTTT‑AACGAATTCGATAATTTTATCGTTCTCATTAACCGCCATAACGCCAAATGCg                                                                                              >  2:4292830/1‑150 (MQ=255)
                                                          gTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTT‑AACGAATTTGGTAATTTTTTCGTTCTCATCAACCGCCATAACGCCCAATGCgg                                                                                             >  2:866262/1‑150 (MQ=255)
                                                            tCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGGTTTTC‑AACGAATTCGATAATTTTTTCGTTCTCATCAACCGCCATAACGCCAAATGCGGAg                                                                                           >  2:1304514/1‑150 (MQ=255)
                                                                 cAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGGTAGCAGGGTTTTT‑AACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGcttc                                                                                      >  2:3455052/1‑150 (MQ=255)
                                                                  aGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTC‑AACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGgttct                                                                                     >  2:3629717/1‑150 (MQ=255)
                                                                  aGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTC‑AACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGcttct                                                                                     >  1:3941685/1‑150 (MQ=255)
                                                                  aGATAGTCGGCGAAAAAGAGGTAGACCCCCATCCCCGCCAGAGTTTTCCTCGGTTCGTTCGGCTTTGGGGGGGGTTTAGCATTTTTTAC‑AACAAATTCTATATTTTTTTCTTTCTCATCACCCGCCATAACGCCAAATGCGGAGGcttct                                                                                     <  2:3479048/150‑1 (MQ=255)
                                                                   gATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGGTTTTC‑AACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCCAATTCGGAGGcttttt                                                                                    >  2:1359028/1‑150 (MQ=255)
                                                                     tAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGGTTTTC‑AACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCa                                                                                  >  1:1011020/1‑150 (MQ=255)
                                                                          ggaaaaaaaGACGTAAACACCCATCCCCGCCAGATTTTTGCGGGGATTGTTCGGCATTGGGGGGGGGTTAGCAGTTTTTCC‑AAAAAATTCGATATTTTTTTCTTTCTCAAAACCCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCgg                                                                             <  2:4629628/144‑1 (MQ=255)
                                                                          ggCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTC‑AACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCgg                                                                             >  1:4624222/1‑150 (MQ=255)
                                                                          ggCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGGTTTTC‑AACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCgg                                                                             >  1:2515691/1‑150 (MQ=255)
                                                                             gTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGGTTTTT‑AACGAAATCGATAATTTTTTCGTTCTCATCAACCGCCATAACGCCCAAAGCGGAGGCTTCTTCAATCGGGAc                                                                          >  2:3392622/1‑150 (MQ=255)
                                                                             gTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGGTTTTC‑AACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTAc                                                                          >  1:2203647/1‑150 (MQ=255)
                                                                                aaaGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTC‑AACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTgg                                                                       >  1:2662407/1‑150 (MQ=255)
                                                                                  aGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTC‑AACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCa                                                                     >  1:1261482/1‑150 (MQ=255)
                                                                                  aGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGGTTTTC‑CACGAATTCGATAATTTTTTCGTTTTCATCAACCGCCATAACGCCAAATGCTGAGGCTTCTTCAATCGGttgttgcc                                                                     >  2:1605296/1‑143 (MQ=255)
                                                                                     cGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGGTAGCAGGTTTTTC‑AACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATAc                                                                  >  1:445157/1‑150 (MQ=255)
                                                                                       tAGATCCCCACCCTCGCGAGAGATTTGCTCGGTTCGTGGGGTATTGGGGGGGGGTTAGCATTTTTTAC‑AAAAATTTCAATTTTTTTATCTTTCACATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACaa                                                                <  2:2977786/150‑1 (MQ=255)
                                                                                        aGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGGTTTTC‑AACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAg                                                               >  2:1244143/1‑150 (MQ=255)
                                                                                         gATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTTGCAGGGTTTTC‑AACGAATTCGATAATTTTTTCGTTCTCATCAACCGCCCTAACGCCAAATGCCGAGGCGTCTTCAATTGGTACTGGCATACAAGc                                                              >  2:1139895/1‑150 (MQ=255)
                                                                                         gATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTC‑AACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGc                                                              >  1:4541413/1‑150 (MQ=255)
                                                                                         gATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTC‑AACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACCCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGc                                                              >  2:4369553/1‑150 (MQ=255)
                                                                                         gATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGGTTGCAGGTTTTTC‑CACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGc                                                              >  1:294442/1‑150 (MQ=255)
                                                                                           cccccATCCCCGCCAGATTTTTGCTGGGATCGTTCGGCATTGGCGGGGGGTTAGCAGGTTTTAA‑AAAAAATTCGATATTTTTATCTTTCTCAACAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCaa                                                            <  2:2925798/148‑1 (MQ=255)
                                                                                              ccATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGGTAGCAGGTTTTTC‑AACGAATTCGATAATTTTTTCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACT‑GCATACCAGCAACGGt                                                        >  1:1180282/1‑150 (MQ=255)
                                                                                                  aCTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGGTTTTC‑AACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCa                                                     >  1:3250311/1‑150 (MQ=255)
                                                                                                        cAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCCGGGTTTTT‑AACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGGATACAAGCAACGGGGCAACGTgc                                               >  2:4054621/1‑150 (MQ=255)
                                                                                                        cAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTC‑AACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGGGCAACGTgc                                               >  2:1989369/1‑150 (MQ=255)
                                                                                                        cAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGGTTTTC‑AACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTgc                                               >  1:2473871/1‑150 (MQ=255)
                                                                                                           agaTTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTC‑AACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATAAAAGCAAAGGGGCAACGTGCGcc                                            >  2:4817913/1‑150 (MQ=255)
                                                                                                             aTTTGCTCGGATCGTTCGGCATTGACGGCGGGGTAGCAGGGTTTTC‑AACGGATTCGGTAATTTTTTCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCtt                                          >  2:557745/1‑150 (MQ=255)
                                                                                                                     ggATCCTGCGGCGTTGAGGGGGGGTTGGCGCTTTTTAA‑AACGAATCCGTTATTTTTTTCGTTATATACAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACg                                  <  2:158131/150‑1 (MQ=255)
                                                                                                                          gTTCGGCATTGACGGCGGGTTAGCAGGGTTTTT‑AACGAATTCGTTAATTTTATCGTTCTCCTCAACCGCCATAACGCCACATGCGGAGGCTTCTTCAATTGGTACTTGACTACAAGCCACGGTGCAACGTGCGGCCTTTTCGACGTGATc                             >  2:598864/1‑150 (MQ=255)
                                                                                                                               gCATTGACGGCGGGTTAGCAGGTTTTTC‑AACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGATaa                        >  1:3687659/1‑150 (MQ=255)
                                                                                                                                 ttggACGGGGGGTTGGGAGTTTTTAC‑AACAAATTCGTTATTTTTTTCTTCCTCACAAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGATAAGc                      <  2:3828258/147‑1 (MQ=255)
                                                                                                                                    gagggggggTTAGCAGTTTTTTC‑AACGATTTAGATATTTTTTTCTTTCTAATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGATAAGCATa                   <  1:2107574/144‑1 (MQ=255)
                                                                                                                                      gggggtgTTAGCGGTTTTTCC‑AACAAATTCAATATTTTTATCTTTCCCACCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGATAAGCATAcg                 <  2:2133135/144‑1 (MQ=255)
                                                                                                                                      cggcggGTTAGCAGGTTTTTC‑AACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGATAAGCATAcg                 >  1:514613/1‑150 (MQ=255)
                                                                                                                                       ggcggGTTAGCAGGTTTTTC‑AACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGATAAGCATAcgc                >  1:4046132/1‑150 (MQ=255)
                                                                                                                                         cggGTTAGCAGGTTTTTC‑AACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGATAAGCATACGCGa              >  1:2862881/1‑150 (MQ=255)
                                                                                                                                           ggTTAGCAGTTTTTTA‑AACGAATTCGATATTTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGATAAGCATACGCGagt            <  1:3299738/150‑1 (MQ=255)
                                                                                                                                           ggTTAGCAGGTTTTTC‑AACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGATAAGCATACGCGagt            >  1:3606972/1‑150 (MQ=255)
                                                                                                                                            gTTAGCAGGTTTTTC‑AACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGATAAGCATACGCGagta           >  2:3065545/1‑150 (MQ=255)
                                                                                                                                             ttAGCATGTTTTCC‑AAAGATTTATATTTTTTTATCGTTCTCATCACCCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGATAAGCATACGCGagtag          <  2:1544239/150‑1 (MQ=255)
                                                                                                                                             ttAGCAGGTTTTTC‑AACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGATAAGCATACGCGagtag          >  2:4714032/1‑150 (MQ=255)
                                                                                                                                              tAGCAGGTTTTTC‑AACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGATAAGCATACGCGagtagt         >  2:3312283/1‑150 (MQ=255)
                                                                                                                                              tAGCAGGTTTTTC‑AACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGATAAGCATACGCGagtagt         >  1:3396905/1‑150 (MQ=255)
                                                                                                                                               aGCAGGTTTTTC‑AACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGATAAGCATACGCGAGTAGTc        >  2:4532491/1‑150 (MQ=255)
                                                                                                                                                gCAGGTTTTTC‑AACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGATAAGCATACGCGAGTAGTct       >  2:355253/1‑150 (MQ=255)
                                                                                                                                                 cAGGTTTTTC‑AACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGATAAGCATACGCGAGTAGTctt      >  1:16092/1‑150 (MQ=255)
                                                                                                                                                  ggtttttaC‑AACGAATTCGATTTTTTTATCCTTCACACCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGATAAGCATACGCGAGTAGTcttg     <  2:2456062/147‑1 (MQ=255)
                                                                                                                                                  cagTTTTTCAAACAATTTCAATATTTTTTTCTTTCTCACCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCAACGTGATCGATAAGCATACGCGAGTAGTctt      <  2:1428674/148‑1 (MQ=255)
                                                                                                                                                     tttttC‑AACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGATAAGCATACGCGAGTAGTcttgctt  >  2:2297803/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                      
ATCAAATCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTC‑AACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGATAAGCATACGCGAGTAGTCTTGCTT  >  AP012306/3020521‑3020819

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑