Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123063,188,0800TG25.8% 426.0 / 20.5 183G177G (GGT→GGG) sgbEL‑ribulose‑5‑phosphate 4‑epimerase
Reads supporting (aligned to +/- strand):  ref base T (41/94);  new base G (47/0);  total (88/95)
Fisher's exact test for biased strand distribution p-value = 2.25e-19
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 244 total aligned reads displayed.

TGCACGCGACAGATGACCGCAGAGGAGATTAACGGCGAATATGAATATCAGACCGGCGAAGTGATCATTGAAACCTTCGAAGAACGTGGCAGGAGTCCGGCACAAATCCCGGCGGTGCTGGTGCATTCT‑CACGGCCCGTTCGCATGGGGTAAAAACGCCGCCGATGCCGTGCATAACGCCGTAGTACTCGAAGAATGCGCCTATATGGGTCTATTCTCGCGCCAGCTTGCGCCGCAGCTCCCTGCGATGCAAAACGAACTGCTGGATAAGCACTACCTGCGTAAGCATGGGGCCAATGC  >  AP012306/3187931‑3188229
                                                                                                                                                      |                                                                                                                                                     
tGCACGCGACAGATGACCGCAGAGGAGATTAACGGCGAATATGAATATCAGACCGGCGAAGTGATCATTGAAACCTTCGAAGAACGTGGCAGGAGTCCGGCACAAATCCCGGCGGTGCTGGTGCATTCT‑CACGGCCCGTTCGCATGGGGt                                                                                                                                                       <  2:1006540/150‑1 (MQ=255)
tGCACGCGACAGATGACCGCAGAGGAGATTAACGGCGAATATGAATATCAGACCGGCGAAGTGATCATTGAAACCTTCGAAGAACGTGGCAGGAGTCCGGCACAAATCCCGGCGGTGCTGGTGCATTCT‑CACGGCCCGTTCGCATGGGGt                                                                                                                                                       <  2:1114443/150‑1 (MQ=255)
tGCACGCGACAGATGACCGCAGAGGAGATTAACGGCGAATATGAATATCAGACCGGCGAAGTGATCATTGAAACCTTCGAAGAACGTGGCAGGAGTCCGGCACAAATCCCGGCGGTGCTGGTGCATTCT‑CACGGCCCGTTCGCATGGGGt                                                                                                                                                       <  2:1585761/150‑1 (MQ=255)
 gCACGCGACAGATGACCGCAGAGGAGATTAACGGCGAATATGAATATCAGACCGGCGAAGTGATCATTGAAACCTTCGAAGAACGTGGCAGGAGTCCGGCACAAATCCCGGCGGTGCTGGTGCATTCT‑CACGGCCCCTTCGCATGGGGTa                                                                                                                                                      >  1:489329/1‑150 (MQ=255)
   aCGCGACAGATGACCGCAGAGGAGATTAACGGCGAATATGAATATCAGACCGGCGAAGTGATCATTGAAACCTTCGAAGAACGTGGCAGGAGTCCGGCACAAATCCCGGCGGTGCTGGTGCATTCT‑CACGGCCCGTTCGCATGGGGTaaa                                                                                                                                                    <  2:3129513/150‑1 (MQ=255)
   aCGCGACAGATGACCGCAGAGGAGATTAACGGCGAATATGAATATCAGACCGGCGAAGTGATCATTGAAACCTTCGAAGAACGTGGCAGGAGTCCGGCACAAATCCCGGCGGTGCTGGTGCATTCT‑CACCGCCCCTTCGCATTGGGGaaa                                                                                                                                                    >  2:2823779/1‑150 (MQ=255)
   aCGCGACAGATGACCGCAGAGGAGATTAACGGCGAATATGAATATCAGACCGGCGAAGTGATCATTGAAACCTTCGAAGAACGTGGCAGGAGTCCGGCACAAATCCCGGCGGGGCCGGGGGATTTTCCCCGGCCCGTTGCCTGGGGGTaa                                                                                                                                                     >  2:2336479/1‑150 (MQ=255)
    cgcgACAGATGACCGCAGAGGAGATTAACGGCGAATATGAATATCAGACCGGCGAAGTGATCATTGAAACCTTCGAAGAACGTGGCAGGAGTCCGGCACAAATCCCGGCGGTGCTGGTGCATTCT‑CACGGCCCGTTCGCATGGGGTaaaa                                                                                                                                                   <  2:926823/150‑1 (MQ=255)
     gcgACAGATGACCGCAGAGGAGATTAACGGCGAATATGAATATCAGACCGGCGAAGTGATCATTGAAACCTTCGAAGAACGTGGCAGGAGTCCGGCACAAATCCCGGCGGTGCTGGTGCATTCT‑CACGGCCCGTTCGCATGGGGTaaaaa                                                                                                                                                  <  2:1678737/150‑1 (MQ=255)
     gcgACAGATGACCGCAGAGGAGATTAACGGCGAATATGAATATCAGACCGGCGAAGTGATCATTGAAACCTTCGAAGAACGTGGCAGGAGTCCGGCACAAATCCCGGCGGGGCTGGTGCATTTT‑CACCGCCCGCTTGCCTGGGGGaaaaa                                                                                                                                                  >  1:3626632/1‑150 (MQ=255)
      cgACAGATGACCGCAGAGGAGATTAACGGCGAATATGAATATCAGACCGGCGAAGTGATCATTGAAACCTTCGAAGAACGTGGCAGGAGTCCGGCACAAATCCCGGCGGTGCTGGTGCATTCT‑CACGGCCCGTTCGCATGGGGTAAAAAc                                                                                                                                                 <  1:2503857/150‑1 (MQ=255)
         cAGATGACCGCAGAGGAGATTAACGGCGAATATGAATATCAGACCGGCGAAGTGATCATTGAAACCTTCGAAGAACGTGGCAGGAGTCCGGCACAAATCCCGGCGGGACTGGTGCATTCT‑CACGGCCCGTTCGCATGGGGTAAAAAcccc                                                                                                                                              >  2:528461/1‑150 (MQ=255)
            aTGACCGCAGAGGAGATTAACGGCGAATATGAATATCAGACCGGCGAAGTGATCATTGAAACCTTCGAAGAACGTGGCAGGAGTCCGGCACAAATCCCGGCGGTGCTGGTGCATTCT‑CACGGCCCGTTCGCATGGGGGAAAAACgccgcc                                                                                                                                           >  1:1427864/1‑150 (MQ=255)
               aCCGAAGAGGAGATTAACGGCGAATATGAATATCAGACCGGCGAAGTGATCATTGAACCCTTCGAAGAACGTGGCAGGAGTCCGGCACAAACCCCGGGGGTGCTGGGGCATTCC‑CCCGGCCCGTTCGCATGGGGTAAAAACGCCGCCGAt                                                                                                                                        <  2:3834167/150‑1 (MQ=255)
                ccGCAGAGGAGATTAACGGCGAATATGAATATCAGACCGGCGAAGTGATCATTGAACCCTTCGAAGAACGTGGCAGGAGTCCGGCACAAATCCCGGCGGTGCTGGTGCATTCT‑CACGGCCCGTTCGCATGGGGTAAAAACGCCGCCGATg                                                                                                                                       <  2:695104/150‑1 (MQ=255)
                   cAGAGGAGATTAACGGCGAATATGAATATCAGACCGGCGAAGTGATCATTGAAACCTTCGAAGAACGTGGCAGGAGTCCGGCACAAATCCCGGCGGTGCTGGTGCATTCT‑CACGGCCCGTTCGCATGGGGTAAAAACGCCGCCGATGCCg                                                                                                                                    <  1:3962536/150‑1 (MQ=255)
                   cAGAGGAGATTAACGGCGAATATGAATATCAGACCGGCGAAGTGATCATTGAAACCTTCGAAGAACGTGGCAGGAGTCCGGCACAAATCCCGGCGGTGCTGGTGCATTCT‑CACGGCCCGTTCGCATGGGGGAAAAACGCCGCCGATGCCg                                                                                                                                    >  1:1210302/1‑150 (MQ=255)
                   cAGAGGAGATTAACGGCGAATATGAATATCAGACCGGCGAAGTGATCATTGAAACCTTCGAAGAACGTGGCAGGAGTCCGGCACAAATCCCGGCGGTGCTGGGGCATTCT‑CACCGCCCGTTTGCCTGGGGGAAAAACCGCGCCCATGGCg                                                                                                                                    >  2:1717740/1‑150 (MQ=255)
                    agagGAGATTAACGGCGAATATGAATATCAGACCGGCGAAGTGATCATTGAAACCTTCGAAGAACGTGGCAGGAGTCCGGCACAAATCCCGGCGTTGCTGGTGCATTCT‑CACGGCCCGTTCGCATGGGGGAAAAAAGCCGCCGATGCCGt                                                                                                                                   >  1:4587433/1‑150 (MQ=255)
                    agagGAGATTAACGGCGAATATGAATATCAGACCGGCGAAGTGATCATTGAAACCTTCGAAGAACGTGGCAGGAGTCCGGCACAAATCCCGGCGGTGCTGGTGCATTCT‑CACGGCCCGTTCGCATGGGGGAAAAAAGCCGCCCATGCCgc                                                                                                                                   >  1:542729/1‑149 (MQ=255)
                       ggagATTAACGGCGAATATGAATATCAGACCGGCGAAGTGATCATTGAAACCTTCGAAGAACGTGGCAGGAGTCCGGCACAAATCCCGGCGGTGCTGGTGCATTCT‑CACCGCCCGTTCGCAATGGGGAAAAACCCCGCCCATTCCCTGcc                                                                                                                                >  1:1670203/1‑149 (MQ=255)
                         agaTTAACGGCGAATATGAATATCAGACCGGCGAAGTGATCATTGAAACCTTCGAAGAACGTGGCAGGAGTCCGGCACAAATCCCGGCGGTGCTGGTGCATTCT‑CACGGCCCGTTCGCATGGGGTAAAAACGCCGCCGATGCCGTGCATa                                                                                                                              <  1:3122104/150‑1 (MQ=255)
                          gaTTAACGGCGAATATGAATATCAGACCGGCGAAGTGATCATTGAAACCTTCGAAGAACGTGGCAGGAGTCCGGCACAAATCCCGGCGGTGCTGGTGCATTCT‑CACGGCCCGTTCGCATGGGGTAAAAACGCCGCCGATGCCGTGCATaa                                                                                                                             <  2:221827/150‑1 (MQ=255)
                           aTTAACGGCGAATATGAATATCAGACCGGCGAAGTGATCATTGAAACCTTCGAAGAACGTGGCAGGAGTCCGGCACAAATCCCGGCGGTGCTGGTGCATTCT‑CACGGCCCGTTCGCATGGGGGAAAAACGCCCCCGATGCCGGGCATAAc                                                                                                                            >  1:4617509/1‑150 (MQ=255)
                           aTTAACGGCGAATATGAATATCAGACCGGCGAAGTGATCATTGAAACCTTCGAAGAACGTGGCAGGAGTCCGGCACAAATCCCGGCGGTGCTGGTGCATTCT‑CACGGCCCCTTCGCATGGGGTAAAAACGCCGCCGATGCCGTGCATAAc                                                                                                                            >  1:4458345/1‑150 (MQ=255)
                             tAACGGCGAATATGAATATCAGACCGGCGAAGTGATCATTGAACCCTTCGAAGAACGTGGCAGGAGTCCGGCACAAATCCCGGCGGTGCTGGTGCTTTCT‑CACGGCCCGTTCGCATGGGGTAAAAACGCCGCCGATGCCGTGCATAACGc                                                                                                                          <  2:2373856/150‑1 (MQ=255)
                             tAACGGCGAATATGAATATCAGACCGGCGAAGTGATCATTGAAACCTTCGAAGAACGTGGCAGGAGTCCGGCACAAATCCCGGCGGTGCTGGTGCATTCT‑CACGGCCCGTTCGCATGGGGGAAAAAAGCCGCCGATGCCGTGCATAACGc                                                                                                                          >  1:860887/1‑150 (MQ=255)
                               aCGGCGAATATGAATATCAGACCGGCGAAGTGATCATTGAAACCTTCGAAGAACGTGGCAGGAGTCCGGCACAAATCCCGGCGGTGCTGGTGCATTCT‑CACGGCCCGTTCGCATGGGGTAAAAACGCCGCCGATGCCGTGCATAACGCCg                                                                                                                        <  1:4264416/150‑1 (MQ=255)
                                cGGCGAATATGAATATCAGACCGGCGAAGTGATCATTGAAACCTTCGAAGAACGTGGCAGGAGTCCGGCACAAATCCCGGCGGTGGTGGGGGATTCT‑CCCGGCCCCGTTGCGTGGGGGAAAAAACCCGCCGGAGCCGGGCATaaaccccg                                                                                                                       >  2:3503343/1‑144 (MQ=255)
                                cGGCGAATATGAATATCAGACCGGCGAAGTGATCATTGAAACCTTCGAAGAACGTGGCAGGAGTCCGGCACAAATCCCGGCGGTGCTGGTGCATTCT‑CACGGCCCGTTCGCATGGGGTAAAAACGCCGCCGATGCCGTGCATAACGCCgt                                                                                                                       <  2:299265/150‑1 (MQ=255)
                                cGGCGAATATGAATATCAGACCGGCGAAGTGATCATTGAAACCTTCGAAGAACGTGGCAGGAGTCCGGCACAAATCCCGGCGGTGCTGGTGCATTCT‑CACGGCCCGTTCGCATGGGGTAAAAACGCCGCCGATGCCGTGCATAACGCCgt                                                                                                                       <  1:2062490/150‑1 (MQ=255)
                                   cGAATATGAATATCAGACCGGCGAAGTGATCATTGAAACCTTCGAAGAACGTGGCAGGAGTCCGGCACAAATCCCGGCGGTGCTGGTGCATTCT‑CACGGCCCGTTCGCATGGGGGAAAAACGCCGCCGATGCCGTGCATAACGCCgtagt                                                                                                                    >  1:2794887/1‑150 (MQ=255)
                                    gaatatgaatatCAGACCGGCGAAGTGATCATTGAAACCTTCGAAGAACGTGGCAGGAGTCCGGCACAAATCCCGGGGGTGCTGGTGCATTCT‑CACGGCCCGTTCGCATGGGGTAAAAACGCCGCCGATGCCGTGCATAACGCCgtagta                                                                                                                   <  1:1024240/150‑1 (MQ=255)
                                    gaatatgaatatCAGACCGGCGAAGTGATCATTGAAACCTTCGAAGAACGTGGCAGGAGTCCGGCACAAATCCCGGCGGTGCTGGGGCATTCT‑CACGGCCCGTTCGCATGGGGGAAAAACGCCGCCGATGCCGTGCATAACGCCggagga                                                                                                                   >  2:448258/1‑148 (MQ=255)
                                    gaatatgaatatCAGACCGGCGAAGTGATCATTGAAACCTTCGAAGAACGTGGCAGGAGTCCGGCACAAATCCCGGCGGGGCTGGTGCATTCT‑CACGGCCCGTTCGCCTGGGGGAAAAACCCCGCCCATGCCCTGCATAAAGCCgtagta                                                                                                                   >  2:1254642/1‑150 (MQ=255)
                                     aatatgaatatCAGACCGGCGAAGTGATCATTGAAACCTTCGAAGAACGTGGCAGGAGTCCGGCACAAATCCCGGCGGTGCTGGTGCATTCT‑CACGGCCCGTTCGCATGGGGTAAAAACGCCGCCGATGCCGTGCATAACGCCGTAGTAc                                                                                                                  <  1:1533526/150‑1 (MQ=255)
                                     aatatgaatatCAGACCGGCGAAGTGATCATTGAAACCTTCGAAGAACGTGGCAGGAGTCCGGCACAAATCCCGGCGGTGCTGGTGCATTCT‑CACGGCCCGTTCGCATGGGGTAAAAACGCCGCCGATGCCGTGCATAACGCCGTAGTAc                                                                                                                  <  2:4042009/150‑1 (MQ=255)
                                     aatatgaatatCAGACCGGCGAAGTGATCATTGAAACCTTCGAAGAACGTGGCAGGAGTCCGGCACAAATCCCGGCGGTGCTGGTGCATTCT‑CACCGCCCGTTCGCCTGGGGGAAAAACGCCCCCGATGCCGTGCCTAACGCCGGAGTAc                                                                                                                  >  1:2048744/1‑150 (MQ=255)
                                     aaagtgaatcgCAGACCGGCGAAGTGACCATTGAACCCTTCGAAGAACGTGGCAGGACACTGACAAAAATCCCGGCGGTGCTGGTGCATTCT‑CACGGCCCGTGCGAATGGGGTAAAAACGCCGCCGATGCCGTGCATAACGCCGTAGTAc                                                                                                                  <  2:4835499/146‑1 (MQ=255)
                                        atgaatatCAGACCGGCGAAGTGATCATTGAAACCTTCGAAGAACGTGGCAGGAGTCCGGCACAAATCCCGGCGGTGCTGGTGCATTCT‑CACGGCCCGTTCGCATGGGGGAAAAACGCCGCCGATGCCGTGCATAACGCCGTAGTACTCg                                                                                                               >  1:4173628/1‑150 (MQ=255)
                                        atgaatatCAGACCGGCGAAGTGATCATTGAAACCTTCGAAGAACGTGGCAGGAGTCCGGCACAAATCCCGGCGGTGCTGGTGCATTCT‑CACGGCCCGTTCGCATGGGGGAAAAACGCCGCCGATGCCGTGCATAACGCCGTAGTACTCg                                                                                                               >  1:4633538/1‑150 (MQ=255)
                                         tgaatatCAGACCGGCGAAGTGATCATTGAAACCTTCGAAGAACGTGGCAGGAGTCCGGCACAAATCCCGGCGGTGCTGGTGCATTCT‑CACGGCCCGTTCGCATGGGGTAAAAACGCCGCCGATGCCGTGCATAACGCCGTAGTACTCga                                                                                                              <  1:2147700/150‑1 (MQ=255)
                                          gaatatCAGACCGGCGAAGTGATCATTGAAACCTTCGAAGAACGTGGCAGGAGTCCGGCACAAATCCCGGCGGTGCTGGTGCATTCT‑CACGGCCCGTTCGCATGGGGTAAAAACGCCGCCGATGCCGTGCATAACGCCGTAGTACTCgaa                                                                                                             <  1:3473823/150‑1 (MQ=255)
                                           aatatCAGACCGGCGAAGTGATCATTGAAACCTTCGAAGAACGTGGCAGGAGTCCGGCACAAATCCCGGCGGTGCTGGTGCATTCT‑CACGGCCCGTTCGCATGGGGGAAAAACGCCGCCGATGCCGTGCATAACGCCGTAGTACTCgaag                                                                                                            >  1:3910066/1‑150 (MQ=255)
                                              atCAGACCGGCGAGGTGATCATTGAAACCGTCGAAGAACGTGGCGGGAGTCCGGCAAAAATCCCGGCGGTGTTGGTGAATTCT‑CACGGCCGGTTCGCAGGGGGAAAAAACGCCGCCGATGCCGTGCATAACGCCGTAGTACTCGAAGAAt                                                                                                         <  2:4207619/150‑1 (MQ=255)
                                                 aGACCGGCGAAGTGATCATTGAAACCTTCGAAGAACGTGGCAGGAGTCCGGCACAAATCCCGGCGGTGCTGGTGCATTCT‑CACGGCCCGTTCGCATGGGGGAAAAACGCCGCCGATGCCGTGCATAACGCCGTAGTAATCGAAGAATgcg                                                                                                      >  1:531324/1‑150 (MQ=255)
                                                  gACCGGCGAAGTGATCATTGAAACCTTCGAAGAACGTGGCAGGAGTCCGGCACAAATCCCGGCGGTGCTGGTGCATTCT‑CACGGCCCGTTCGCATGGGGTAAAAACGCCGCCGATGCCGTGCATAACGCCGTAGTACTCGAAGAATgcgc                                                                                                     >  1:759580/1‑150 (MQ=255)
                                                   aCCGGCGAAGTGATCATTGAAACCTTCGAAGAACGTGGCAGGAGTCCGGCACAAATCCCGGGGGTGCTGGTGCATTCT‑CACGGCCCGTTCGCATGGGGTAAAAACGCCGCCGATGCCGTGCATAACGCCGTAGTACTCGAAGAATGCGcc                                                                                                    <  2:364205/150‑1 (MQ=255)
                                                    ccGGCGAAGTGATCATTGAAACCTTCGAAGAACGTGGCAGGAGTCCGGCACAAATCCCGGCGGTGCTGGTGCATTCT‑CACGGCCCGTTCGCATGGGGTAAAAACGCCGCCGATGCCGTGCATAACGCCGTAGTACTCGAAGAATGCGCCt                                                                                                   <  1:2579925/150‑1 (MQ=255)
                                                    ccGGCGAAGTGATCAGTGAAACCTTCGAAGAACGTGGCAGGAGTCCGGCACAAACCCCGGGGGTGGTGGTGCATTCT‑CACGGCCCGTTCGCATGGGGTAAAAACGCCGCCGATGCCGTGCATAACGCCGTAGTACTCGAAGAATGCGCCt                                                                                                   <  2:3152080/150‑1 (MQ=255)
                                                     cGGCGAAGTGATCATTGAAACCTTCGAAGAACGTGGCAGGAGTCCGGCACAAATCCCGGCGGTGCTGGTGCATTCT‑CACGGCCCGTTCGCATGGGGTAAAAACGCCGCCGATGCCGTGCATAACGCCCTAGTACTCGAAGAATGCGCCta                                                                                                  >  1:4050817/1‑150 (MQ=255)
                                                     cGGCAAAATGATCATTGAAACCTTCGAAGAACGTGGCAGGAGTCCGGCACAAATCCCGGCGTTGCTGGTGCATTCT‑CACGGCCCCTTCCCAGGGGGAAAAAACGCCGCCGATGCCGTGCATAACGCCGTAGTTCTCGAAGAATGCGCCtt                                                                                                  <  2:3420077/150‑2 (MQ=255)
                                                      ggCGAAGTGATCATTGAAACCTTCGAAGAACGTGGCAGGAGGCCGGCACAAATCCCGGCGGTGGTGGTGCAGTCT‑CACGGCCCGGTCGCATGGGGGAAAAACGCCGCCGGTGCCGGGGATAAAGCCGTAGGAATCGAAGAATGGGCCcat                                                                                                 >  2:4785607/1‑150 (MQ=255)
                                                        cGAAGTGATCATTGAAACCTTCGAAGAACGTGGCAGGAGTCCGGCACAAATCCCGGCGGTGCTGGTGCATTCT‑CACGGCCCGTTCGCATTGGGGAAAAACGCCGCCGATGCCGTGCATAAAGCCGTAGTACTCGAAGAATGCGCCTatat                                                                                               >  1:2325006/1‑150 (MQ=255)
                                                        cGAAGTGATCATTGAAACCTTCGAAGAACGTGGCAGGAGTCCGGCACAAATCCCGGCGGTGCTGGTGCATTCT‑CACGGCCCGTTCGCATGGGGTAAAAACGCCGCCGATGCCGTGCATAACGCCGTAGTACTCGAAGAATGCGCCTatat                                                                                               <  2:3938701/150‑1 (MQ=255)
                                                             tGATCATTGAAACCTTCGAAGAACGTGGCAGGAGTCCGGCACAAATCCCGGCGGTGCTGGTGCATTCT‑CACGGCCCGTTCGCATGGGGGAAAAACGCCGCCGATGCCGTGCATAACGCCGTAGTACTCGAAGAATGCGCCTATATGGGTc                                                                                          >  1:4666492/1‑150 (MQ=255)
                                                             tGATCATTGAAACCTTCGAAGAACGTGGCAGGAGTCCGGCACAAATCCCGGCGGGGCTGGGGCATTCT‑CACGGCCCGGTCGCATGGGGGAAAAACGCCGCCGGTGCCGGGCATAACGCCGGAGGACTCGAAGAAAGCGCCTATATggggc                                                                                          >  2:312079/1‑148 (MQ=255)
                                                               aTCATTGAAACCTTCGAAGAACGTGGCAGGAGTCCGGCACAAATCCCGGCGGTGCTGGTGCATTCT‑CACGGCCCGTTCGCATGGGGGAAAAACGCCGCCGATGCCGTGCATAAAGCCGTAGTACTCGAAAAAAGCGCCCATATGGGGCTa                                                                                        >  2:1545762/1‑150 (MQ=255)
                                                                 cATTGAAACCTTCGAAGAACGTGGCAGGAGTCCGGCACAAATCCCGGCGGTGCTGGTGCATTCT‑CACGGCCCGGTCGCATGGGGTAAAAACGCCGCCGATGCCGTGCATAACGCCCGAGTACTCGAAGAATGCGCCTATATGGGGCTAtt                                                                                      >  2:4431972/1‑150 (MQ=255)
                                                                  aTTGAAACCTTCGAAGAACGTGGCAGGAGTCCGGCACAAATCCCGGCGGTGCTGGTGCATTCT‑CACGGCCCGTTCGCATGGGGTAAAAACGCCGCCGATGCCGTGCATAACGCCGTAGTACTCGAAGAATGCGCCTATATGGGTCTATtc                                                                                     <  1:2206572/150‑1 (MQ=255)
                                                                     gAAACCTTCGAAGAACGTGGCAGGAGTCCGGCACAAATCCCGGCGGTGCTGGTGCATTCT‑CACGGCCCGTTCGCATGGGGGAAAAACGCCGCCGATGCCGTGCATAACGCCGTAGTACTCGAAGAATGCGCCTATATGGGGCTATTCTcg                                                                                  >  2:2719718/1‑150 (MQ=255)
                                                                             cGAAGAACGTGGCAGGAGTCCGGCACAAATCCCGGCGGTGCTGGTGCATTCT‑CACGGCCCGTTCGCATGGGGGAAAAACGCCGCCGATGCCGTGCATAACGCCGTAGTACTCGAAGAATGCGCCTATATGGGTCTATTCTCGCGCCAGCt                                                                          >  1:1858421/1‑150 (MQ=255)
                                                                               aagaaCGTGGCAGGAGTCCGGCACAAATCCCGGCGGTGCTGGTGCATTCT‑CACGGCCCGTTCGCATGGGGTAAAAACGCCGCCGATGCCGTGCATAACGCCGTAGTACTCGAAGAATGCGCCTATATGGGTCTATTCTCGCGCCAGCTTg                                                                        <  1:3522084/150‑1 (MQ=255)
                                                                               aagaaCGTGGCAGGAGTCCGGCACAAATCCCGGCGGTGCTGGGGCATTCT‑CACGGCCCGGTCGCATGGGGGAAAAACGCCGCCCATGCCCTGCCTAACGCCGTAGGACTCGAAGAAAGCGCCCATATTGGGCTATTTTCCCGCCAGCCTg                                                                        >  2:4747919/1‑150 (MQ=255)
                                                                                   aCGTGGCAGGAGTCCGGCACAAATCCCGGCGGTGCTGGTGCATTCT‑CACGGCCCGTTCGCATGGGGGAAAAACGCCGCCGATGCCGTGCATAACGCCGTAGTACTCGAAGAATGCGCCTATATGGGTCTATTCTCGCGCCAGCTTGcgcc                                                                    >  1:4486752/1‑150 (MQ=255)
                                                                                      tGGCAGGAGTCCGGCACAAATCCCGGCGGTGCTGGGGCATTCT‑CACGGCCCGGTCGCCTGGGGGAAAAACGCCCCCGATGCCGTGCATAAAGCCGTAGTACTCGAAAAAAGGGCCCATATTGGGCTATTTTCCCGCCCGCTTTCGCCCCa                                                                 >  2:4888618/1‑150 (MQ=255)
                                                                                       ggCAGGAGTCCGGCACAAATCCCGGCGGTGCTGGTGCATTCT‑CACCGCCCGGTCGCATGGGGGAAAAAAGCCGCCGGTGCCGTGCATAACCCCGGAGTAACCCAAAAAAGCGCCCATATGGGTCCATTCTCGCCCCCGCTTTCGCCCCaa                                                                >  2:896876/1‑149 (MQ=255)
                                                                                        gCAGGAGTCCGGCACAAATCCCGGCGGTGCTGGGGCATTCT‑CACGGCCCGTTCGCATGGGGTAAAACCGCCGCCGATGCCGTGCATAACGCCGTAGTACTCGAAGAATGCGCCTATATGGGTCTATTCTCGCGCCAGCTTGCGCCGCAGc                                                               <  2:4310380/150‑1 (MQ=255)
                                                                                         cAGGAGTCCGGCACAAATCCCGGCGGTGCTGGTGCATTCT‑CACGGCCCGTTCGCATGGGGTAAAAACGCCGCCGATGCCGTGCATAACGCCGTAGTACTCGAAGAATGCGCCTATATGGGTCTATTCTCGCGCCAGCTTGCGCCGCAGCt                                                              <  2:2268283/150‑1 (MQ=255)
                                                                                          aGGAGTCCGGCACAAATCCCGGCGGTGCTGGTGCATTCT‑CACGGCCCGTTCGCATGGGGTAAAAACGCCGCCGATGCCGTGCATAACGCCGTAGTACTCGAAGAATGCGCCTATATGGGTCTATTCTCGCGCCAGCTTGCGCCGCAGCTc                                                             >  1:3745212/1‑150 (MQ=255)
                                                                                          aGGAGTCCGGCACAAATCCCGGCGGTGCTGGTGCATTCT‑CACGGCCCGTTCGCATGGGGTAAAAACGCCGCCGATGCCGTGCATAACGCCGTAGTACTCGAAGAATGCGCCTATATGGGTCTATTCTCGCGCCAGCTTGCGCCGCAGCTc                                                             <  2:2897395/150‑1 (MQ=255)
                                                                                           ggAGTCCGGCACAAATCCCGGCGGTGCTGGTGCATTCT‑CACGGCCCGTTCGCATGGGGGAAAAACGCCGCCGATGCCGTGCATAACGCCGTAGTACTCGAAGAAAGCGCCTATATGGGGCTATTCTCGCGCCAGCTTGCGCCGCAGCTcc                                                            >  2:1878110/1‑150 (MQ=255)
                                                                                           ggAGTCCGGCACAAATCCCGGCGGTGCTGGGGCATTCT‑CACGGCCCGGTCGCATGGGGGAAAAACGCCGCCGATGCCGTGCATAACGCCGTAGGACTCGAAGAATGCGCCTATATGGGTCTATTCTCGCGCCAGCTTGCGCCGCAGCTcc                                                            >  1:1736434/1‑150 (MQ=255)
                                                                                            gAGTCCGGCACAAATCCCGGCGGTGCTGGTGCATTCT‑CACGGCCCGTTCGCATGGGGTAAAAACCCCGCCGATGCCGTGCATAACGCCGTAGTACTCGAAGAATGCGCCTATATGGGTCTATTCTCGCGCCCGCTTGCGCCGCAGCTccc                                                           >  2:141612/1‑150 (MQ=255)
                                                                                             aGTCCGGCACAAATCCCGGCGGTGCTGGTGCATTCT‑CACGGCCCGTTCGCATGGGGGAAAAACGCCGCCGATGCCGTGCATAACGCCGTAGTACTCGAAGAATGCGCCTATATGGGTCTATTCTCGCGCCAGCTTGCGCCGCAGCTCCCt                                                          >  2:2627773/1‑150 (MQ=255)
                                                                                             aGTCCGGCACAAATCCCGGCGGTGCTGGTGCATTCT‑CACGGCCCGTTCGCATGGGGGAAAAACGCCGCCGATGCCGTGCATAACGCCGTAGTACTCGAAGAATGCGCCTATATGGGTCTATTCTCGCGCCAGCTTGCGCCGCAGCTCCCt                                                          >  1:2679919/1‑150 (MQ=255)
                                                                                              gTCCGGCACAAATCCCGGCGGTGCTGGTGCATTCT‑CACGGCCCGTTCGCATGGGGTAAAAACGCCGCCGATGCCGTGCATAACGCCGTAGTACTCGAAGAATGCGCCTATATGGGTCTATTCTCGCGCCAGCTTGCGCCGCAGCTCCCTg                                                         <  2:3013524/150‑1 (MQ=255)
                                                                                              gTCCGGCACAAATCCCGGCGGTGCTGGTGCATTCT‑CACGGCCCGTTCGCATGGGGTAAAAACGCCGCCGATGCCGTGCATAACGCCGTAGTACTCGAAGAATGCGCCTATATGGGTCTATTCTCGCGCCAGCTTGCGCCGCAGCTCCCTg                                                         <  1:2545711/150‑1 (MQ=255)
                                                                                                    aacaAATCCCGGCGGTGCTGGTGCATGCC‑CTGGGCCCGTTCGCATGGGGTAAAAACGCCGCCGATGCCGTGCATAACGCCGTAGTACTCGAAGAATGCGCCTATATGGGTCTATTCTCGCGCCAGCTTGCGCCGCAGCTCCCTGCGATGc                                                   <  2:3245333/149‑1 (MQ=255)
                                                                                                     acaAATCCCGGCGGTGCTGGTGCATTCT‑CACGGCCCCTTCGCATGGGGGAAAAACGCCGCCGATGCCGTGCATAACCCCGTAGTACTCGAAGAATGCGCCTATATGGGTCTATTCTCGCGCCAGCTTGCGCCGCAGCCCCCTGCGATGCa                                                  >  1:1950105/1‑150 (MQ=255)
                                                                                                      caAATCCCGGCGGTGCTGGTGCATTCT‑CACGGCCCGTTCGCATGGGGGAAAAACGCCGCCGATGCCGTGCATAACGCCGTAGTACTCGAAGAATGCGCCTATATGGGTCTATTCTCGCGCCAGCTTGCGCCGCAGCTCCCTGCGATGCaa                                                 >  1:3390435/1‑150 (MQ=255)
                                                                                                         aTCCCGGCGGTGCTGGTGCATTCT‑CACGGCCCGTTCGCATGGGGGAAAAACGCCGCCGGTGCCGTGCATAACGCCGTAGTACTCGAAGAATGCGCCTATATGGGGCTATTCTCGCGCCCGCTTGCGCCGCAGCTCCCTGCGATGCaaaaa                                              >  2:1797558/1‑149 (MQ=255)
                                                                                                                  gTGCTGGTGCATTCT‑CACGGCCCGTTCGCATGGGGTAAAAACGCCGCCGATGCCGTGCATAACGCCGTAGTACTCGAAGAATGCGCCTATATGGGTCTATTCTCGCGCCAGCTTGCGCCGCAGCTCCCTGCGATGCAAAACGAActgctg                                     <  2:1576218/150‑1 (MQ=255)
                                                                                                                     cTGGTGCATTCT‑CACGGCCCGTTCGCATGGGGTAAAAACGCCGCCGATGCCGTGCATAACGCCGTAGTACTCGAAGAATGCGCCTATATGGGTCTATTCTCGCGCCAGCTTGCGCCGCAGCTCCCTGCGATGCAAAACGAACTGCTGGAt                                  >  1:4385835/1‑150 (MQ=255)
                                                                                                                       ggTGCATTCT‑CACGGCCCGTTCGCATGGGGTAAAAACGCCGCCGATGCCGTGCATAACGCCGTAGTACTCGAAGAATGCGCCTATATGGGTCTATTCTCGCGCCAGCTTGCGCCGCAGCTCCCTGCGATGCAAAACGAACTGCTGGATaa                                >  1:2854365/1‑150 (MQ=255)
                                                                                                                        gTGCATTCT‑CACGGCCCGTTCGCATGGGGTAAAAACGCCGCCGATGCCGTGCATAACGCCGTAGTACTCGAAGAATGCGCCTATATGGGTCTATTCTCGCGCCAGCTTGCGCCGCAGCTCCCTGCGATGCAAAACGAACTGCTGGATAAg                               >  1:4327309/1‑150 (MQ=255)
                                                                                                                         tGCATTCT‑CACGGCCCGTTCGCATGGGGTAAAAACGCCGCCGATGCCGTGCATAACGCCGTAGTACTCGAAGAATGCGCCTATATGGGTCTATTCTCGCGCCAGCTTGCGCCGCAGCTCCCTGCGATGCAAAACGAACTGCTGGATAAGc                              <  2:3787189/150‑1 (MQ=255)
                                                                                                                           cATTCT‑CACGGCCCGTTCGCATGGGGTAAAAACGCCGCCGATGCCGTGCATAACGCCGTAGTACTCGAAGAATGCGCCTATATGGGTCTATTCTCGCGCCAGCTTGCGCCGCAGCTCCCTGCGATGCAAAACGAACTGCTGGATAAGCAc                            <  1:2987564/150‑1 (MQ=255)
                                                                                                                            aTTCT‑CACGGCCCGTTCGCATGGGGTAAAAACGCCGCCGATGCCGTGCATAACGCCGTAGTACTCGAAGAATGCGCCTATATGGGTCTATTCTCGCGCCAGCTTGCGCCGCAGCTCCCTGCGATGCAAAACGAACTGCTGGATAAGCACt                           <  1:23928/150‑1 (MQ=255)
                                                                                                                               ct‑cACGGCCCGTTCGCATGGGGTAAAACCGCCGCCGATGCCGTGCATAACGCCGTAGTACTCGAAGAATGCGCCTATATGGGTCTATTCTCGCGCCAGCTTGCGCCGCAGCTCCCTGCGATGCAAAACGAACTGCTGGATAAGCACTAcc                        <  2:3314539/150‑1 (MQ=255)
                                                                                                                               ct‑cACGGCCCGTTCGCATGGGGTAAAAACGCCGCCGATGCCGTGCATAACGCCGTAGTACTCGAAGAATGCGCCTATATGGGTCTATTCTCGCGCCAGCTTGCGCCGCAGCTCCCTGCGATGCAAAACGAACTGCTGGATAAGCACTAcc                        >  1:4717958/1‑150 (MQ=255)
                                                                                                                                      gCCCGTTCGCATGGGGTAAAAGCGGCGCCGATGCCGTGCATAACGCCGTAGTACTCGAAGAATGCGCCTATATGGGTCTATTCTCGCGCCAGCTTGCGCCGCAGCTCCCTGCGATGCAAAACGAACTGCTGGATAAGCACTACCTGCGTa                  <  2:2012090/150‑1 (MQ=255)
                                                                                                                                         cGTTCGCATGGGGTAAAAACGCCGCCGATGCCGTGCATAACGCCGTAGTACTCGAAGAATGCGCCTATATGGGTCTATTCTCGCGCCAGCTTGCGCCGCAGCTCCCTGCGATGCAAAACGAACTGCTGGATAAGCACTACCTGCGTAAGc               >  1:4024642/1‑150 (MQ=255)
                                                                                                                                           ttCGCATGGGGTAAAAACGCCGCCGATGCCGTGCATAACGCCGTAGTACTCGAAGAATGCGCCTATATGGGTCTATTCTCGCGCCAGCTTGCGCCGCAGCTCCCTGCGATGCAAAACGAACTGCTGGATAAGCACTACCTGCGTAAGCAt             <  1:369105/150‑1 (MQ=255)
                                                                                                                                              gCATGGGGTAAAAACGCCGCCGATGCCGTGCATAACGCCGTAGTACTCGAAGAATGCGCCTATATGGGTCTATTCTCGCGCCAGCTTGCGCCGCAGCTCCCTGCGATGCAAAACGAACTGCTGGATAAGCACTACCTGCGTAAGCATggg          >  1:1912792/1‑150 (MQ=255)
                                                                                                                                               cATGGGGTAAAAACGCCGCCGATGCCGTGCATAACGCCGTAGTACTCGAAGAATGCGCCTATATGGGTCTATTCTCGCGCCAGCTTGCGCCGCAGCTCCCTGCGATGCAAAACGAACTGCTGGATAAGCACTACCTGCGTAAGCATgggg         >  2:724615/1‑150 (MQ=255)
                                                                                                                                                 tGGGGTAAAAACGCCGCCGATGCCGTGCATAACGCCGTAGTACTCGAAGAATGCGCCTATATGGGTCTATTCTCGCGCCAGCTTGCGCCGCAGCTCCCTGCGATGCAAAACGAACTGCTGGATAAGCACTACCTGCGTAAGCATGGGGcc       <  1:3298931/150‑1 (MQ=255)
                                                                                                                                                     gTAAAAACGCCGCCGATGCCGTGCATAACGCCGTAGTACTCGAAGAATGCGCCTATATGGGTCTATTCTCGCGCCAGCTTGCGCCGCAGCTCCCTGCGATGCAAAACGAACTGCTGGATAAGCACTACCTGCGTAAGCATGGGGCCAATg   >  1:694416/1‑150 (MQ=255)
                                                                                                                                                     gTAAAAACGCCGCCGATGCCGTGCATAACGCCGTAGTACTCGAAGAATGCGCCTATATGGGTCTATTCTCGCGCCAGCTTGCGCCGCAGCTCCCTGCGATGCAAAACGAACTGCTGGATAAGCACTACCTGCGTAAGCATGGGGCCAATg   >  1:4851607/1‑150 (MQ=255)
                                                                                                                                                     gTAAAAACGCCGCCGATGCCGTGCATAACGCCGTAGTACTCGAAGAATGCGCCTATATGGGTCTATTCTCGCGCCAGCTTGCGCCGCAGCTCCCTGCGATGCAAAACGAACTGCTGGATAAGCACTACCTGCGTAAGCATGGGGCCAATg   <  1:1052345/150‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                     
TGCACGCGACAGATGACCGCAGAGGAGATTAACGGCGAATATGAATATCAGACCGGCGAAGTGATCATTGAAACCTTCGAAGAACGTGGCAGGAGTCCGGCACAAATCCCGGCGGTGCTGGTGCATTCT‑CACGGCCCGTTCGCATGGGGTAAAAACGCCGCCGATGCCGTGCATAACGCCGTAGTACTCGAAGAATGCGCCTATATGGGTCTATTCTCGCGCCAGCTTGCGCCGCAGCTCCCTGCGATGCAAAACGAACTGCTGGATAAGCACTACCTGCGTAAGCATGGGGCCAATGC  >  AP012306/3187931‑3188229

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑