Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123063,639,0400TG24.4% 165.0 / 15.3 78noncoding (54/76 nt)gltVtRNA‑Glu
Reads supporting (aligned to +/- strand):  ref base T (2/57);  new base G (19/0);  total (21/57)
Fisher's exact test for biased strand distribution p-value = 3.13e-16
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 150 total aligned reads displayed.

CACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCA‑CTTGCTGGTTTGTGAGTGAAAGTCACCTGCCTTAA  >  AP012306/3638892‑3639097
                                                                                                                                                    |                                                          
cACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGtt                                                           <  2:4226716/150‑1 (MQ=32)
 aCCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGGTc                                                          >  1:1891484/1‑150 (MQ=17)
 aCCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAAGGGGTc                                                          >  1:3863016/1‑150 (MQ=17)
  cctcctTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACTTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCg                                                         <  2:4725386/150‑1 (MQ=17)
  cctcctTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGGTCg                                                         >  1:4708365/1‑150 (MQ=17)
   ctcctTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGa                                                        >  1:3016600/1‑150 (MQ=32)
   ctcctTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAAGGGTTCGa                                                        >  1:4430711/1‑150 (MQ=17)
     ccttaccttaAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAAt                                                      <  1:1745144/150‑1 (MQ=32)
      cttaccttaAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATc                                                     <  2:3125053/150‑1 (MQ=32)
       ttaccttaAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATcc                                                    <  1:7857/150‑1 (MQ=32)
        taccttaAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGGTCGAAAccc                                                   >  1:1568481/1‑150 (MQ=17)
        taccttaAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGGAACAGGGGGTCCAATccc                                                   >  1:2792882/1‑150 (MQ=12)
          ccttaAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGGTCGCAAccccc                                                 >  2:3472153/1‑149 (MQ=12)
          ccttaAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGGTCGAATCCCCt                                                 >  2:3449288/1‑150 (MQ=17)
          ccttaAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGGTCGAATCCCCt                                                 >  1:3863/1‑150 (MQ=17)
          ccttaAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGGTCGAATCCCCt                                                 >  2:2919935/1‑150 (MQ=17)
          ccttaAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAAAAGGGGGTCGAAAccccc                                                 >  2:1284752/1‑149 (MQ=12)
          ccttaAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGGAAAAAGGGGTCGGATCCCCt                                                 >  2:165258/1‑150 (MQ=11)
           cttaAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTa                                                <  2:98714/150‑1 (MQ=32)
           cttaAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGGTCGAATCCCCTa                                                >  1:1951266/1‑150 (MQ=17)
            ttaAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGGTCGAATCCCCTat                                               >  2:1394112/1‑149 (MQ=17)
            ttaAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGAGGGTCGAATCCCCCAg                                               >  2:828648/1‑150 (MQ=12)
             taAAGAAGCGTTCTTTGCAGTGCTCACACAGATTTTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACACGGGGTTGAAACCCCTAgg                                              >  1:2454054/1‑150 (MQ=11)
             taAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAgg                                              <  1:4496750/150‑1 (MQ=32)
             taAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGGTCGAATCCCCTAgg                                              >  1:4645210/1‑150 (MQ=17)
             taAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGGTCGAATCCCCCAgg                                              >  1:1056540/1‑150 (MQ=17)
              aaaGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAggg                                             <  1:2920225/150‑1 (MQ=32)
              aaaGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAggg                                             <  2:4019537/150‑1 (MQ=32)
              aaaGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGGTTGAATCCCCTAggg                                             >  2:4154065/1‑150 (MQ=17)
                 gaagCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGGTCGAATCCCCTAGGGGAc                                          >  1:646395/1‑150 (MQ=17)
                   agCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAAGGGGTCGAATCCCCTAGGGGgcgc                                        >  2:3149548/1‑150 (MQ=12)
                   agCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAAGGGGTCGAATCCCCCAGGGGACGc                                        >  1:3948814/1‑150 (MQ=12)
                    gCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGGTCGAATCCCCCAGGGGACGcc                                       >  2:2657/1‑150 (MQ=17)
                    gCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAAGGGGTCGGATCCCCCAgggggctcc                                       >  2:1828165/1‑145 (MQ=11)
                    gCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGCTACACAGGCTCCGATCCCCTAGGGGGCGcc                                       >  1:1110440/1‑150 (MQ=11)
                     cGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTGACAAGGGGTCGGATCCCCTTAGGGGCGCCa                                      >  2:3711655/1‑150 (MQ=11)
                      gTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGGTCGAATCCCCTAGGGGACGcccc                                     >  1:3055361/1‑148 (MQ=17)
                      gTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGGTCGAATCCCCTAGGGGACGCCA‑c                                    >  1:4718116/1‑150 (MQ=17)
                      gTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGGTCGAATCCCCTAGGGGACGCCA‑c                                    >  2:174068/1‑150 (MQ=17)
                      gTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGGTCGAATCCCCCAGGGGACGCCA‑c                                    >  1:3107020/1‑150 (MQ=17)
                        tCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCA‑Ctt                                  <  2:4601655/150‑1 (MQ=32)
                        tCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAAAAAGGGGTCGAAACCCCCAGGGGGCGccccct                                   >  1:3655348/1‑146 (MQ=11)
                         cTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGGTCGGATCCCCTAGGGGCCGCCA‑CCTg                                 >  1:4289703/1‑150 (MQ=255)
                         cTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAAGGGGTCGAATCCCCTAGGGGGCGccccctg                                  >  1:1042569/1‑145 (MQ=255)
                          tttGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCA‑CTTGc                                <  1:1818607/150‑1 (MQ=32)
                          tttGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGGTCCAATTCCCTAGGGGGCGcccccttc                                 >  1:2257623/1‑144 (MQ=11)
                           ttGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCA‑CTTGCt                               <  2:1490786/150‑1 (MQ=32)
                              cAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCA‑CTTGCTGGt                            <  1:3569320/150‑1 (MQ=32)
                              cAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCA‑CTTGCTGGt                            <  1:3519905/150‑1 (MQ=32)
                              cAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGGTCGAATCCCCTAGGGGGCGCCA‑CTTGCTGGt                            >  2:2573003/1‑150 (MQ=17)
                              cAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAAGGGGTCGAATCCCCCCAGGGGCGCCC‑CTTGCTggg                            >  1:3723112/1‑149 (MQ=11)
                               aGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCA‑CTTGCTGGtt                           <  2:9308/150‑1 (MQ=32)
                               aGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCA‑CTTGCTGGtt                           <  1:2073277/150‑1 (MQ=32)
                                gTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCA‑CTTGCTGGttt                          <  2:4642486/150‑1 (MQ=32)
                                gTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGGTCGAATCCCCTAGGGGACGCCC‑CTTTCTGGttt                          >  1:170042/1‑150 (MQ=12)
                                   cTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCA‑CTTGCTGGTTtgtg                       <  1:593870/150‑1 (MQ=32)
                                   cTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGGTCGAATCCCCTCGGGGGCGCCC‑CCTGCTGGGTtgtg                       >  2:2312255/1‑150 (MQ=11)
                                   cTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGGTCGAATCCCCTAGGGGGCGCCA‑CTTGCTGGTTtttg                       >  1:3260691/1‑150 (MQ=12)
                                    tCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACATGGGGTCGAATTCCCCAGGGGGCGCCA‑CCTGCTGGGTtgtgg                      >  1:4865528/1‑149 (MQ=11)
                                     cacacaGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCA‑CTTGCTGGTTTgtgag                     <  1:4432853/150‑1 (MQ=32)
                                     cacacaGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAAGGGGTCGAATCCCCCAGGGGGCGCCA‑CCTGCTGGTTTgtgag                     >  1:1735572/1‑150 (MQ=11)
                                      acacaGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCA‑CTTGCTGGTTTgtgagt                    >  1:2625581/1‑150 (MQ=32)
                                      acacaGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCA‑CTTGCTGGGTTgtgagt                    >  1:702080/1‑150 (MQ=17)
                                      acacaGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGGTCGAATCCCCTAGGGGACGCCA‑CTTGCTGGGTTttgagt                    >  1:755551/1‑150 (MQ=12)
                                      acacaGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGGTCGAATCCCCCAGGGGGCGCCC‑CTTTCTGGTTTgtgagg                    >  2:1865309/1‑149 (MQ=11)
                                       cacaGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCA‑CTTGCTGGTTTgtgagtg                   <  2:3652739/150‑1 (MQ=32)
                                       cacaGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCA‑CTTGCTGGTTTgtgagtg                   <  1:1486939/150‑1 (MQ=32)
                                       cacaGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAAGGGGTCGAATACCCTAGGGGGCGCCA‑CTTGCTGGTTTgtgagtg                   >  2:827674/1‑150 (MQ=255)
                                        acaGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCA‑CTTGCTGGTTTgtgagtga                  <  1:2063405/150‑1 (MQ=32)
                                        acaGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCA‑CTTGCTGGTTTgtgagtga                  <  2:3402905/150‑1 (MQ=32)
                                        acaGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAAAAGGGGGTCGAAACCCCTAGGGGACGCCA‑CTTGCTGGGTTgtgaggga                  >  1:1591508/1‑150 (MQ=11)
                                        acaGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGGAACAGGGGGGGGGAACCCCCAGGGGGCGCCA‑CTTGCTGGGTTgtgaggga                  >  2:3771489/1‑150 (MQ=11)
                                         caGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGGTCCCATCCCCCACGGGACGCCACCTTGC‑GGTTTttgaaggaa                 >  1:3367180/1‑145 (MQ=11)
                                         caGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAAGGGGTCGAATCCCCTAGGGGGCGCCC‑CTTGCTGGTTTGTGAGTGaa                 >  2:3038914/1‑150 (MQ=255)
                                          aGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCA‑CTTGCTGGTTTGTGAGTGaaa                <  1:2546492/150‑1 (MQ=32)
                                          aGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGGTCGAATCCCCCAGGGGGCGCCC‑CTTGCTGGGTTGTGAGGGaaa                >  2:2781698/1‑150 (MQ=11)
                                          aGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAAGGGGTCGAATCCCCCAGGGGGCGCCC‑CTTGCTGGTTTGTGAGTGgaa                >  1:4370475/1‑150 (MQ=11)
                                          aGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGAAAAAGGGGGTCCAATACCCCCGGGGCAGCCA‑CCTTCTGGGTTGTGGGTGaaa                >  2:2858306/1‑150 (MQ=11)
                                            aTTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAAGGGGTCAGAACCCCTCCGGGGCGCCA‑CTTTGTGGGTTGTGTGAGaaaaa              >  2:2676049/1‑148 (MQ=11)
                                            aTTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAAAAGGGGTTCGAATCCCCTTGGGGGGGCCC‑CTTTCTTGTTTGTGAGGGGAAGt              >  2:3450622/1‑150 (MQ=11)
                                             ttGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCA‑CTTGCTGGTTTGTGAGTGAAAGTc             <  1:1716483/150‑1 (MQ=32)
                                              tGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCA‑CTTGCTGGTTTGTGAGTGAAAGTCa            <  2:2454714/150‑1 (MQ=32)
                                              tGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCA‑CTTGCTGGTTTGTGAGTGAAAGTCa            <  2:1552204/150‑1 (MQ=32)
                                              tGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGGTCCAATCCCCTAGGGGACGCCA‑CTTGCTGGTTTGTGAGTGAAAGTCa            >  1:1314609/1‑150 (MQ=17)
                                              tGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGGAAAAGGGGGTCGAATCCCCTAGGGGGCGCCA‑CTTGCTGGGTTGTGGGTGAAAATCa            >  1:609399/1‑150 (MQ=11)
                                               gTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGCAACAGGGGGTCGAAACCCCTAGGGGGCGCCC‑CTTGCTGGGTTGTGAGGGAAAGTCAc           >  1:601414/1‑150 (MQ=11)
                                                tCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCA‑CTTGCTGGTTTGTGAGTGAAAGTCAcc          <  1:1645479/150‑1 (MQ=32)
                                                tCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGGTCGAATCCCCTAGGGGACGCCA‑CTTGCTGGTTTGTGAGTGAAAGTCAcc          >  1:1296563/1‑150 (MQ=17)
                                                 cttaTAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTTATAAGGGGTCGGATCCCCCCCCGGGGGCCC‑CTTACCGGTTTTTGTGTGGAAATCACCt         >  2:4364915/4‑150 (MQ=11)
                                                 cTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAAGGGGTCGAATCCCCCCGGGGGCGCCC‑CCTTCTGGTTTTTGAGTGaaaaggacct         >  2:4224191/1‑143 (MQ=11)
                                                   gATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCA‑CTTGCTGGTTTGTGAGTGAAAGTCACCTGc       <  1:3976903/150‑1 (MQ=32)
                                                    aTAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGGTCGAATCCCCTAGGGGGCGCCA‑CTTGCTGGTTTGTGAGTGAAAGTCACCTGcc      >  1:2908450/1‑150 (MQ=17)
                                                    aTAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAAGGGGTCGAATCCCCCAAGGGGCGCCC‑CTTGCTGGTTTGTGAGGGAAAGTCACCTGcc      >  2:4412976/1‑150 (MQ=11)
                                                     tAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCA‑CTTGCTGGTTTGTGAGTGAAAGTCACCTGCCt     <  1:3373230/150‑1 (MQ=32)
                                                      aGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCA‑CTTGCTGGTTTGTGAGTGAAAGTCACCTGCCtt    <  1:1034863/150‑1 (MQ=32)
                                                      aGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCA‑CTTGCTGGTTTGTGAGTGAAAGTCACCTGCCtt    <  1:1457336/150‑1 (MQ=32)
                                                      aGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGGTCGAATCCCCCAGGGGACGCCA‑CTTGCTGGGTTGTGAGTGAAAGTCACCTGccct    >  2:3400838/1‑148 (MQ=12)
                                                       gAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCA‑CTTGCTGGTTTGTGAGTGAAAGTCACCTGCCTTa   <  1:2693700/150‑1 (MQ=31)
                                                        aaaGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCA‑CTTGCTGGTTTGTGAGTGAAAGTCACCTGCCTTaa  <  2:485700/150‑1 (MQ=11)
                                                        aaaGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCA‑CTTGCTGGTTTGTGAGTGAAAGTCACCTGCCTTaa  <  2:2868552/150‑1 (MQ=11)
                                                                                                                                                    |                                                          
CACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCA‑CTTGCTGGTTTGTGAGTGAAAGTCACCTGCCTTAA  >  AP012306/3638892‑3639097

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑