BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AP012306	2,316,362	Δ1 bp	coding (1013/1203 nt)	proV →	glycine betaine transporter subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AP012306	2,316,361	0	G	.	100.0%	1343.8 / NA	270	coding (1012/1203 nt)	proV	glycine betaine transporter subunit
Reads supporting (aligned to +/- strand): ref base G (0/0); new base . (114/156); total (114/156)

Only 100 of 281 total aligned reads displayed.

TCGTAAAACCCCTGGCTTCGGCCCACGTTCGGCACTGAAATTATTGCAGGATGAAGATCGCGAATATGGCTACGTTATCGAACGCGGTAATAAGTTTGTCGGCGCAGTCTCCATCGATTCGCTTAAAACCGCGTTAACGCAGCAGCAAGGTCTTGATGCGGCGCTGATTGATGCGCCGTTAGCAGTCGATGCACAAACGCCTCTTAGCGAGTTGCTCTCTCATGTCGGACAGGCACCCTGTGCGGTGCCCGTGGTCGACGAGGACCAACAGTATGTCGGCATCATTTCGAAAGG  >  AP012306/2316213‑2316506

tCGTAAAACCCCTGGCTTCGGCCCACGTTCGGCACTGAAATTATTGCAGGATGAAGATCGCGAATATGGCTACGTTATCGAACGCGGTAATAAGTTTGTCGGCGCAGTCTCCATCGATTCGCTTAAAACCGCGTTAACGCAGCAGCAAgt                                                                                                                                                  <  1:908551/150‑2 (MQ=255)

 cGTAAAACCCCTGGCTTCGGCCCACGTTCGGCACTGAAATTATTGCAGGATGAAGATCGCGAATATGGCTACGTTATCGAACGCGGTAATAAGTTTGTCGGCGCAGTCTCCATCGATTCGCTTAAAACCGCGTTAACGCAGCAGCAAgtc                                                                                                                                                 >  1:2373554/1‑148 (MQ=255)

  gTAAAACCCCTGGCTTCGGCCCACGTTCGGCACTGAAATTATTGCAGGATGAAGATCGCGAATATGGCTACGTTATCGAACGCGGTAATAAGTTTGTCGGCGCAGTCTCCATCGATTCGCTTAAAACCGCGTTAACGCAGCAGCAAgtct                                                                                                                                                <  2:4431402/150‑4 (MQ=255)

      aaCCCCTGGCTTCGGCCCACGTTCGGCACTGAAATTATTGCAGGATGAAGATCGCGAATATGGCTACGTTATCGAACGCGGTAATAAGTTTGTCGGCGCAGTCTCCATCGATTCGCTTAAAACCGCGTTAACGCAGCAGCAA‑GTCTTGAt                                                                                                                                           >  2:2557605/1‑150 (MQ=255)

          ccTGGCTTCGGCCCACGTTCGGCACTGAAATTATTGCAGGATGAAGATCGCGAATATGGCTACGTTATCGAACGCGGTAATAAGTTTGTCGGCGCAGTCTCCATCGATTCGCTTAAAACCGCGTTAACGCAGCAGCAA‑GTCTTGATgcgg                                                                                                                                       <  1:1842989/150‑1 (MQ=255)

           cTGGCTTCGGCCCACGTTCGGCACTGAAATTATTGCAGGATGAAGATCGCGAATATGGCTACGTTATCGAACGCGGTAATAAGTTTGTCGGCGCAGTCTCCATCGATTCGCTTAAAACCGCGTTAACGCAGCAGCAA‑GTCTTGATgcggc                                                                                                                                      <  1:4251242/150‑1 (MQ=255)

           cTGGCTTCGGCCCACGTTCGGCACTGAAATTATTGCAGGATGAAGATCGCGAATATGGCTACGTTATCGAACGCGGTAATAAGTTTGTCGGCGCAGTCTCCATCGATTCGCTTAAAACCGCGTTAACGCAGCAGCAA‑GTCTTGATgcggc                                                                                                                                      >  2:2735069/1‑150 (MQ=255)

              gCTTCGGCCCACGTTCGGCACTGAAATTATTGCAGGATGAAGATCGCGAATATGGCTACGTTATCGAACGCGGTAATAAGTTTGTCGGCGCAGTCTCCATCGATTCGCTTAAAACCGCGTTAACGCAGCAGCAA‑GTCTTGATGCGGCGCt                                                                                                                                   <  2:2330580/150‑1 (MQ=255)

               cTTCGGCCCACGTTCGGCACTGAAATTATTGCAGGATGAAGATCGCGAATATGGCTACGTTATCGAACGCGGTAATAAGTTTGTCGGCGCAGTCTCCATCGATTCGCTTAAAACCGCGTTAACGCAGCAGCAA‑GTCTTGATGCGGCGCtg                                                                                                                                  <  2:2941807/150‑1 (MQ=255)

                ttCGGCCCACGTTCGGCACTGAAATTATTGCAGGATGAAGATCGCGAATATGGCTACGTTATCGAACGCGGTAATAAGTTTGTCGGCGCAGTCTCCATCGATTCGCTTAAAACCGCGTTAACGCAGCAGCAA‑GTCTTGATGCGGCGCtga                                                                                                                                 >  1:3587460/1‑150 (MQ=255)

                  cGGCCCACGTTCGGCACTGAAATTATTGCAGGATGAAGATCGCGAATATGGCTACGTTATCGAACGCGGTGATAAGTTTGTCGGCGCAGTCTCCATCGATTCGCTTAAAACCGCGTTAACGCAGCAGCAA‑GTCTTGATGCGGCGCtgatt                                                                                                                               <  1:2019147/150‑1 (MQ=255)

                   ggCCCACGTTCGGCACTGAAATTATTGCAGGATGAAGATCGCGAATATGGCTACGTTATCGAAAGCGGTAATAAGTTTGTCGGCGCAGTCTCCATCGATTCGCTTAAAACCGCGTTAACGCAGCAGCAA‑GTCTTGATGCGGCGCtgattg                                                                                                                              <  2:557527/150‑1 (MQ=255)

                    gCCCACGTTCGGCACTGAAATTATTGCAGGATGAAGATCGCGAATATGGCTACGTTATCGAACGCGGTAATAAGTTTGTCGGCGCAGTCTCCATCGATTCGCTTAAAACCGCGTTAACGCAGCAGCAA‑GTCTTGATGCGGCGCtgattga                                                                                                                             <  1:295301/150‑1 (MQ=255)

                      ccACGTTCGGCACTGAAATTATTGCAGGATGAAGATCGCGAATATGGCTACGTTATCGAACGCGGT‑ATAAGTTTGTCGGCGCAGTCTCCATCGATTCGCTTAAAACCGCGTTAACGCAGCAGCAA‑GTCTTGATGCGGCGCTGATTGATgc                                                                                                                          <  1:2841822/150‑1 (MQ=255)

                       cACGTTCGGCACTGAAATTATTGCAGGATGAAGATCGCGAATATGGCTACGTTATCGAACGCGGTAATAAGTTTGTCGGCGCAGTCTCCATCGATTCGCTTAAAACCGCGTTAACGCAGCAGCAA‑GTCTTGATGCGGCGCTGATTGATgc                                                                                                                          <  1:2870891/150‑1 (MQ=255)

                         cGTTCGGCACTGAAATTATTGCAGGATGAAGATCGCGAATATGGCTACGTTATCGAACGCGGTAATAAGTTTGTCAGCGCAGTCTCCATCGATTCGCTTAAAACCGCGTTAACGCAGCAGCAA‑GTCTTGATGCGGCGCTGATTGATgcgc                                                                                                                        >  2:3230047/1‑150 (MQ=255)

                               gCACTGAAATTATTGCAGGATGAAGATCGCGAATATGGCTACATTATCGAACGCGGTAATAAGTTTGTCGGCGCAGTCTCCATCGATTCGCTTAAAACCGCGTTAACGCAGCAGCAA‑GTCTTGATGCGGCGCTGATTGATGCGCCGTTAg                                                                                                                  <  2:3987746/150‑1 (MQ=255)

                                      aaTTATTGCAGGATGAAGATCGCGAATATGGCTACGTTATCGAACGCGGTAATAAGTTTGTCGGCGCAGTCTCCATCGATTCGCTTAAAACCGCGTTAACGCAGCAGCAA‑GTCTTGATGCGGCGCTGATTGATGCGCCGTTAGCAGTCGa                                                                                                           >  2:1971712/1‑150 (MQ=255)

                                        ttattGCAGGATGAAGATCGCGAATATGGCTACGTTATCGAACGCGGTAATAAGTTTGTCGGCGCAGTCTCCATCGATTCGCTTAAAACCGCGTTAACGCAGCAGCAA‑GTCTTGATGCGGCGCTGATTGATGCGCCGTTAGCAGTCGATg                                                                                                         <  2:1314979/150‑1 (MQ=255)

                                         tattGCAGGATGAAGATCGCGAATATGGCTACGTTATCGAACGCGGTAATAAGTTTGTCGGCGCAGTCTCCATCGATTCGCTTAAAACCGCGTTAACGCAGCAGCAA‑GTCTTGATGCGGCGCTGATTGATGCGCCGTTAGCAGTCGATGc                                                                                                        <  2:100119/150‑1 (MQ=255)

                                              cAGGATGAAGATCGCGAATATGGCTACGTTATCGAACGCGGTAATAAGTTTGTCGGCGCAGTCTCCATCGATTCGCTTAAAACCGCGTTAACGCAGCAGCAA‑GTCTTGATGCGGCGCTGATTGATGCGCCGTTAGCAGTCGATGCACaaa                                                                                                   <  1:3090518/150‑1 (MQ=255)

                                               aGGATGAAGATCGCGAATATGGCTACGTTATCGAACGCGGTAATAAGTTTGTCGGCGCAGTCTCCATCGATTCGCTTAAAACCGCGTTAACGCAGCAGCAA‑GTCTTGATGCGGCGCTGATTGATGCGCCGTTAGCAGTCGATGCACAAAc                                                                                                  <  1:4098926/150‑1 (MQ=255)

                                                ggATGAAGATCGCGAATATGGCTACGTTATCGAACGCGGTAATAAGTTTGTCGGCGCAGTCTCCATCGATTCGCTTAAAACCGCGTTAACGCAGCAGCAA‑GTCTTGATGCGGCGCTGATTGATGCGCCGTTAGCAGTCGATGCACAAACg                                                                                                 >  2:1809049/1‑150 (MQ=255)

                                                   tGAAGATCGCGAATATGGCTACGTTATCGACCGCGGCAATAAGTTTGTCTGCGCAGTCTCCATCGATTCGCTTAAAACCGCGTTAACGCATCAGCAA‑GACTTGATGCGGCGCTGATTGATGGGCCGTTAGCAGTCGATGAACAAACGCct                                                                                              >  2:2728316/1‑150 (MQ=255)

                                                   tGAAGATCGCGAATATGGCTACGTTATCGAACGCGGTAATAAGTTTGTCGGCGCAGTCTCCATCGATTCGCTTAAAACCGCGTTAACGCAGCAGCAA‑GTCTTGATGCGGCGCTGATTGATGCGCCGTTAGCAGTCGATGCACAAACGCct                                                                                              <  2:3711533/150‑1 (MQ=255)

                                                   tGAAGATCGCGAATATGGCTACGTTATCGAACGCGGTAATAAGTTTGTCGGCGCAGTCTCCATCGATTCGCTTAAAACCGCGTTAACGCAGCAGCAA‑GTCTTGATGCGGCGCTGATTGATGCGCCGTTAGCAGTCGATGCACAAACGCct                                                                                              >  2:2232375/1‑150 (MQ=255)

                                                    gAAGATCGCGAATATGGCTACGTTATCGAACGCGGTAATAAGTTTGTCGGCGCAGTCTCCATCGATTCGCTTAAAACCGCGTTAACGCAGCAGCAA‑GTCTTGATGCGGCGCTGATTGATGCGCCGTTAGCAGTCGATGCACAAACGCctc                                                                                             <  1:4004885/150‑1 (MQ=255)

                                                     aaGATCGCGAATATGGCTACGTTATCGAACGCGGTAATAAGTTTGTCGGCGCAGTCTCCATCGATTCGCTTAAAACCGCGTTAACGCAGCAGCAA‑GTCTTGATGCGGCGCTGATTGATGCGCCGTTAGCAGTCGATGCACAAACGCctct                                                                                            >  2:4572333/1‑150 (MQ=255)

                                                     aaGATCGCGAATATGGCTACGTTATCGAACGCGGTAATAAGTTTGTCGGCGCAGTCTCCATCGATTCGCTTAAAACCGCGTTAACGCAGCAGCAA‑GTCTTGATGCGGCGCTGATTGATGCGCCGTTAGCAGTCGATGCACAAACGCctct                                                                                            >  2:904156/1‑150 (MQ=255)

                                                       gATCGCGAATATGGCTACGTTATCGAACGCGGTAATAAGTTTGTCGGCGCAGTCTCCATCGATTCGCTTAAAACCGCGTTAACGCAGCAGCAA‑GTCTTGATGCGGCGCTGATTGATGCGCCGTTAGCAGTCGATGCACAAACGCCTCTTa                                                                                          >  2:3358150/1‑150 (MQ=255)

                                                        aTCGCGAATATGGCTACGTTATCGAACGCGGTAATAAGTTTGTCGGCGCAGTCTCCATCGATTCGCTTAAAACCGCGTTAACGCAGCAGCAA‑GTCTTGATGCGGCGCTGATTGATGCGCCGTTAGCAGTCGATGCACAAACGCCTCTTAg                                                                                         <  1:1684961/150‑1 (MQ=255)

                                                        aTCGCGAATATGGCTACGTTATCGAACGCGGTAATAAGTTTGTCGGCGCAGTCTCCATCGATTCGCTTAAAACCGCGTTAACGCAGCAGCAA‑GTCTTGATGCGGCGCTGATTGATGCGCCGTTAGCAGTCGATGCACAAACGCCTCTTAg                                                                                         <  2:2673655/150‑1 (MQ=255)

                                                         tCGCGAATATGGCTACGTTATCGAACGCGGTAATAAGTTTGTCGGCGCAGTCTCCATCGATTCGCTTAAAACCGCGTTAACGCAGCAGCAA‑GTCTTGATGCGGCGCTGATTGATGCGCCGTTAGCAGTCGATGCACAAACGCCTCTTAGc                                                                                        <  1:1017698/150‑1 (MQ=255)

                                                           gcgAATATGGCTACGTTATCGAACGCGGTAATAAGTTTGTCGGCGCAGTCTCCATCGATTCGCTTAAAACCGCGTTAACGCAGCAGCAA‑GTCTTGATGCGGCGCTGATTGATGCGCCGTTAGCAGTCGATGCACAAACGCCTCTTAGCGa                                                                                      <  2:4406867/150‑1 (MQ=255)

                                                             gAATATGGCTACGTTATCGAACGCGGTAATAAGTTTGTCGGCGCAGTCTCCATCGATTCGCTTAAAACCGCGTTAACGCAGCAGCAA‑GTCTTGATGCGGCGCTGATTGATGCGCCGTTAGCAGTCGATGCACAAACGCCTCTTAGCGAGt                                                                                    >  1:2391854/1‑150 (MQ=255)

                                                               atatGGCTACGTTATCGAACGCGGTAATAAGTTTGTCGGCGCAGTCTCCATCGATTCGCTTAAAACCGCGTTAACGCAGCAGCAA‑GTCTTGATGCGGCGCTGATTGATGCGCCGTTAGCAGTCGATGCACAAACGCCTCTTAGCGAGTTg                                                                                  >  1:225464/1‑150 (MQ=255)

                                                                 atGGCTACGTTATCGAACGCGGTAATAAGTTTGTCGGCGCAGTCTCCATCGATTCGCTTAAAACCGCGTTAACGCAGCAGCAA‑GTCTTGATGCGGCGCTGATTGATGCGCCGTTAGCAGTCGATGCACAAACGCCTCTTAGCGAGTTGct                                                                                <  2:1594438/150‑1 (MQ=255)

                                                                    gCTACGTTATCGAACGCGGTAATAAGTTTGTCGGCGCAGTCTCCATCGATTCGCTTAAAACCGCGTTAACGCAGCAGCAA‑GTCTTGATGCGGCGCTGATTGATGCGCCGTTAGCAGTCGATGCACAAACGCCTCTTAGCGAGTTGctctc                                                                             <  2:685888/150‑1 (MQ=255)

                                                                      tACGTTATCGAACGCGGTAATAAGTTTGTCGGCGCAGTCTCCATCGATTCGCTTAAAACCGCGTTAACGCAGCAGCAA‑GTCTTGATGCGGCGCTGATTGATGCGCCGTTAGCAGTCGATGCACAAACGCCTCTTAGCGAGTTGCtctctc                                                                           >  2:3167280/1‑150 (MQ=255)

                                                                         gTTATCGAACGCGGTAATAAGTTTGTCGGCGCAGTCTCCATCGATTCGCTTAAAACCGCGTTAACGCAGCAGCAA‑GTCTTGATGCGGCGCTGATTGATGCGCCGTTAGCAGTCGATGCACAAACGCCTCTTAGCGAGTTGCTCTCTCATg                                                                        >  1:3755581/1‑150 (MQ=255)

                                                                         gTTATCGAACGCGGTAATAAGTTTGTCGGCGCAGTCTCCATCGATTCGCTTAAAACCGCGTTAACGCAGCAGCAA‑GTCTTGATGCGGCGCTGATTGATGCGCCGTTAGCAGTCGATGCACAAACGCCTCTTAGCGAGTTGCTCTCTCATg                                                                        >  1:1104076/1‑150 (MQ=255)

                                                                         gTTATCGAACGCGGTAATAAGTTTGTCGGCGCAGTCTCCATCGATTCGCTTAAAACCGCGTTAACGCAGCAGCAA‑GTCTTGATGCGGCGCTGATTGATGCGCCGTTAGCAGTCGATGCACAAACGCCTCTTAGCGAGTTGCTCTCTCATg                                                                        >  1:2505385/1‑150 (MQ=255)

                                                                         gTTATCGAACGCGGTAATAAGTTTGTCGGCGCAGTCTCCATCGATTCGCTTAAAACCGCGTTAACGCAGCAGCAA‑GTCTTGATGCGGCGCTGATTGATGCGCCGTTAGCAGTCGATGCACAAACGCCTCTTAGCGAGTTGCTCTCTCATg                                                                        >  1:3775782/1‑150 (MQ=255)

                                                                          ttATCGAACGCGGTAATAAGTTTGTCGGCGCAGTCTCCATCGATTCGCTTAAAACCGCGTTAACGCAGCAGCAA‑GTCTTGATGCGGCGCTGATTGATGCGCCGTTAGCAGTCGATGCACAAACGCCTCTTAGCGAGTTGCTCTCTCATGt                                                                       >  1:1230983/1‑150 (MQ=255)

                                                                           tATCGAACGCGGTAATAAGTTTGTCGGCGCAGTCTCCATCGATTCGCTTAAAACCGCGTTAACGCAGCAGCAA‑GTCTTGATGCGGCGCTGATTGATGCGCCGTTAGCAGTCGATGCACAAACGCCTCTTAGCGAGTTGCTCTCTCATGTc                                                                      <  2:2592796/150‑1 (MQ=255)

                                                                            aTCGAACGCGGTAATAAGTTTGTCGGCGCAGTCTCCATCGATTCGCTTAAAACCGCGTTAACGCAGCAGCAA‑GTCTTGATGCGGCGCTGATTGATGCGCCGTTAGCAGTCGATGCACAAACGCCTCTTAGCGAGTTGCTCTCTCATGTCg                                                                     <  2:4102799/150‑1 (MQ=255)

                                                                            aTCGAACGCGGTAATAAGTTTGTCGGCGCAGTCTCCATCGATTCGCTTAAAACCGCGTTAACGCAGCAGCAA‑GTCTTGATGCGGCGCTGATTGATGCGCCGTTAGCAGTCGATGCACAAACGCCTCTTAGCGAGTTGCTCTCTCATGTCg                                                                     >  2:3297932/1‑150 (MQ=255)

                                                                              cGAACGCGGTAATAAGTTTGTCGGCGCAGTCTCCATCGATTCGCTTAAAACCGCGTTAACGCAGCAGCAA‑GTCTTGATGCGGCGCTGATTGATGCGCCGTTAGCAGTCGATGCACAAACGCCTCTTAGCGAGTTGCTCTCTCATGTCGGa                                                                   <  1:4576874/150‑1 (MQ=255)

                                                                               gAACGCGGTAATAAGTTTGTCGGCGCAGTCTCCATCGATTCGCTTAAAACCGCGTTAACGCAGCAGCAA‑GTCTTGATGCGGCGCTGATTGATGCGCCGTTAGCAGTCGATGCACAAACGCCTCTTAGCGAGTTGCTCTCTCATGTCGGAc                                                                  <  1:4390143/150‑1 (MQ=255)

                                                                                 aCGCGGTAATAAGTTTGTCGGCGCAGTCTCCATCGATTCGCTTAAAACCGCGTTAACGCAGCAGCAA‑GTCTTGATGCGGCGCTGATTGATGCGCCGTTAGCAGTCGATGCACAAACGCCTCTTAGCGAGTTGCTCTCTCATGTCGGACAg                                                                <  2:82100/150‑1 (MQ=255)

                                                                                 aCGCGGTAATAAGTTTGTCGGCGCAGTCTCCATCGATTCGCTTAAAACCGCGTTAACGCAGCAGCAA‑GTCTTGATGCGGCGCTGATTGATGCGCCGTTAGCAGTCGATGCACAAACGCCTCTTAGCGAGTTGCTCTCTCATGTCGGACAg                                                                <  2:17289/150‑1 (MQ=255)

                                                                                     ggTAATAAGTTTGTCGGCGCAGTCTCCATCGATTCGCTTAAAACCGCGTTAACGCAGCAGCAA‑GTCTTGATGCGGCGCTGATTGATGCGCCGTTAGCAGTCGATGCACAAACGCCTCTTAGCGAGTTGCTCTCTCATGTCGGACAGGCAc                                                            <  1:3447272/150‑1 (MQ=255)

                                                                                      gTAATAAGTTTGTCGGCGCAGTCTCCATCGATTCGCTTAAAACCGCGTTAACGCAGCAGCAA‑GTCTTGATGCGGCGCTGATTGATGCGCCGTTAGCAGTCGATGCACAAACGCCTCTTAGCGAGTTGCTCTCTCATGTCGGACAGGCAcc                                                           <  1:3519951/150‑1 (MQ=255)

                                                                                          taaGTTTGTCGGCGCAGTCTCCATCGATTCGCTTAAAACCGCGTTAACGCAGCAGCAA‑GTCTTGATGCGGCGCTGATTGATGCGCCGTTAGCAGTCGATGCACAAACGCCTCTTAGCGAGTTGCTCTCTCATGTCGGACAGGCACCCtgt                                                       >  2:1635297/1‑150 (MQ=255)

                                                                                           aaGTTTGTCGGCGCAGTCTCCATCGATTCGCTTAAAACCGCGTTAACGCAGCAGCAA‑GTCTTGATGCGGCGCTGATTGATGCGCCGTTAGCAGTCGATGCACAAACGCCTCTTAGCGAGTTGCTCTCTCATGTCGGACAGGCACCCtgtg                                                      >  2:117853/1‑150 (MQ=255)

                                                                                           aaGTTTGTCGGCGCAGTCTCCATCGATTCGCTTAAAACCGCGTTAACGCAGCAGCAA‑GTCTTGATGCGGCGCTGATTGATGCGCCGTTAGCAGTCGATGCACAAACGCCTCTTAGCGAGTTGCTCTCTCATGTCGGACAGGCACCCtgtg                                                      >  2:3125830/1‑150 (MQ=255)

                                                                                            aGTTTGTCGGCGCAGTCTCCATCGATTCGCTTAAAACCGCGTTAACGCAGCAGCAA‑GTCTTGATGCGGCGCTGATTGATGCGCCGTTAGCAGTCGATGCACAAACGCCTCTTAGCGAGTTGCTCTCTCATGTCGGACAGGCACCCTGTGc                                                     >  1:1742762/1‑150 (MQ=255)

                                                                                             gTTTGTCGGCGCAGTCTCCATCGATTCGCTTAAAACCGCGTTAACGCAGCAGCAA‑GTCTTGATGCGGCGCTGATTGATGCGCCGTTAGCAGTCGATGCACAAACGCCTCTTAGCGAGTTGCTCTCTCATGTCGGACAGGCACCCTGTGCg                                                    >  2:1857055/1‑150 (MQ=255)

                                                                                              tttGTCGGCGCAGTCTCCATCGATTCGCTTAAAACCGCGTTAACGCAGCAGCAA‑GTCTTGATGCGGCGCTGATTGATGCGCCGTTAGCAGTCGATGCACAAACGCCTCTTAGCGAGTTGCTCTCTCATGTCGGACAGGCACCCTGTGCgg                                                   >  1:3252930/1‑150 (MQ=255)

                                                                                              tttGTCGGCGCAGTCTCCATCGATTCGCTTAAAACCGCGTTAACGCAGCAGCAA‑GTCTTGATGCGGCGCTGATTGATGCGCCGTTAGCAGTCGATGCACAAACGCCTCTTAGCGAGTTGCTCTCTCATGTCGGACAGGCACCCTGTGCgg                                                   <  2:1358147/150‑1 (MQ=255)

                                                                                              tttGTCGGCGCAATCTCCATCGATTCGCTTAAAACCGCGTTAACGCAGCAGCAA‑GTCTTGATGCGGCGCTGATTGATGCGCCGTTAGCAGTCGATGCACAAACGCCTCTTAGCGAGTTGCTCTCTCATGTCGGACAGGCACCCTGTGCgg                                                   <  1:391115/150‑1 (MQ=255)

                                                                                                tGTCGGCGCAGTCTCCATCGATTCGCTTAAAACCGCGTTAACGCAGCAGCAA‑GTCTTGATGCGGCGCTGATTGATGCGCCGTTAGCAGTCGATGCACAAGCGCCTCTTAGCGAGTTGCTCTCTCATGTCGGACAGGCACCCTGTGCGGTg                                                 <  2:3768497/150‑1 (MQ=255)

                                                                                                tGTCGGCGCAGTCTCCATCGATTCGCTTAAAACCGCGTTAACGCAGCAGCAA‑GTCTTGATGCGGCGCTGATTGATGCGCCGTTAGCAGTCGATGCACAACCGCCTCTTAGCGAGTTGCTCTCTCATGTCGGACAGGCACCCTGTGCGGTg                                                 >  2:2026759/1‑150 (MQ=255)

                                                                                                tGTCGGCGCAGTCTCCATCGATTCGCTTAAAACCGCGTTAACGCAGCAGCAA‑GTCTTGATGCGGCGCTGATTGATGCGCCGTTAGCAGTCGATGCACAAACGCCTCTTAGCGAGTTGCTCTCTCATGTCGGACAGGCACCCTGTGCGGTg                                                 >  2:4146580/1‑150 (MQ=255)

                                                                                                  tCGGCGCAGTCTCCATCGATTCGCTTAAAACCGCGTTAACGCAGCAGCAA‑GTCTTGATGCGGCGCTGATTGATGCGCCGTTAGCAGTCGATGCACAAACGCCTCTTAGCGAGTTGCTCTCTCATGTCGGACAGGCACCCTGTGCGGTGcc                                               >  2:3254331/1‑150 (MQ=255)

                                                                                                  tCGGCGCAGTCTCCATCGATTCGCTTAAAACCGCGTTAACGCAGCAGCAA‑GTCTTGATGCGGCGCTGATTGATGCGCCGTTAGCAGTCGATGCACAAACGCCTCTTAGCGAGTTGCTCTCTCATGTCGGACAGGCACCCTGTGCGGTGcc                                               >  1:1631118/1‑150 (MQ=255)

                                                                                                    ggCGCAGTCTCCATCGATTCGCTTAAAACCGCGTTAACGCAGCAGCAA‑GTCTTGATGCGGCGCTGATTGATGCGCCGTTAGCAGTCGATGCACAAACGCCTCTTAGCGAGTTGCTCTCTCATGTCGGACAGGCACCCTGTGCGGTGCCCg                                             >  2:3428161/1‑150 (MQ=255)

                                                                                                    ggCGCAGTCTCCATCGATTCGCTTAAAACCGCGTTAACGCAGCAGCAA‑GTCTTGATGCGGCGCTGATTGATGCGCCGTTAGCAGTCGATGCACAAACGCCTCTTAGCGAGTTGCTCTCTCATGTCGGACAGGCACCCTGTGCGGTGCCCg                                             >  2:527550/1‑150 (MQ=255)

                                                                                                     gcgcAGTCTCCATCGATTCGCTTAAAACCGCGTTAACGCAGCAGCAA‑GTCTTGATGCGGCGCTGATTGATGCGCCGTTAGCAGTCGATGCACAAACGCCTCTTAGCGAGTTGCTCTCTCATGTCGGACAGGCACCCTGTGCGGTGCCCGt                                            <  2:3740266/150‑1 (MQ=255)

                                                                                                     gcgcAGTCTCCATCGATTCGCTTAAAACCGCGTTAACGCAGCAGCAA‑GTCTTGATGCGGCGCTGATTGATGCGCCGTTAGCAGTCGATGCACAAACGCCTCTTAGCGAGTTGCTCTCTCATGTCGGACAGGCACCCTGTGCGGTGCCCGt                                            <  2:3076946/150‑1 (MQ=255)

                                                                                                       gcAGTCTCCATCGATTCGCTTAAAACCGCGTTAACGCAGCAGCAA‑GTCTTGATGCGGCGCTGATTGATGCGCCGTTAGCAGTCGATGCACAAACGCCTCTTAGCGAGTTGCTCTCTCATGTCGGACAGGCACCCTGTGCGGTGCCCGTgg                                          >  1:1906520/1‑150 (MQ=255)

                                                                                                        cAGTCTCCATCGATTCGCTTAAAACCGCGTTAACGCAGCAGCAA‑GTCTTGATGCGGCGCTGATTGATGCGCCGTTAGCAGTCGATGCACAAACGCCTCTTAGCGAGTTGCTCTCTCATGTCGGACAGGCACCCTGTGCGGTGCCCGTGGt                                         <  1:4312602/150‑1 (MQ=255)

                                                                                                         aGTCTCCATCGATTCGCTTAAAACCGCGTTAACGCAGCAGCAA‑GTCTTGATGCGGCGCTGATTGATGCGCCGTTAGCAGTCGATGCACAAACGCCTCTTAGCGAGTTGCTCTCTCATGTCGGACAGGCACCCTGTGCGGTGCCCGTGGTc                                        <  2:3099745/150‑1 (MQ=255)

                                                                                                             tcCATCGATTCGCTTAAAACCGCGTTAACGCAGCAGCAA‑GTCTTGATGCGGCGCTGATTGATGCGCCGTTAGCAGTCGATGCACAAACGCCTCTTAGCGAGTTGCTCTCTCATGTCGGACAGGCACCCTGTGCGGTGCCCGTGGTcgacg                                    >  2:287923/1‑150 (MQ=255)

                                                                                                               cATCGATTCGCTTAAAACCGCGTTAACGCAGCAGCAA‑GTCTTGATGCGGCGCTGATTGATGCGCCGTTAGCAGTCGATGCACAAACGCCTCTTAGCGAGTTGCTCTCTCATGTCGGACAGGCACCCTGTGCGGTGCCCGTGGTCGACGAg                                  <  1:610097/150‑1 (MQ=255)

                                                                                                                 tCGATTCGCTTAAAACCGCGTTAACGCAGCAGCAA‑GTCTTGATGCGGCGCTGATTGATGCGCCGTTAGCAGTCGATGCACAAACGCCTCTTAGCGAGTTGCTCTCTCATGTCGGACAGGCACCCTGTGCGGTGCCCGTGGTCGACGAGGa                                <  1:696692/150‑1 (MQ=255)

                                                                                                                  cGATTCGCTTAAAACCGCGTTAACGCAGCAGCAA‑GTCTTGATGCGGCGCTGATTGATGCGCCGTTAGCAGTCGATGCACAAACGCCTCTTAGCGAGTTGCTCTCTCATGTCGGACAGGCACCCTGTGCGGTGCCCGTGGTCGACGAGGAc                               <  1:1043523/150‑1 (MQ=255)

                                                                                                                     ttCGCTTAAAACCGCGTTAACGCAGCAGCAA‑GTCTTGATGCGGCGCTGATTGATGCGCCGTTAGCAGTCGATGCACAAACGCCTCTTAGCGAGTTGCTCTCTCATGTCGGACAGGCACCCTGTGCGGTGCCCGTGGTCGACGAGGACCaa                            <  2:431495/150‑1 (MQ=255)

                                                                                                                      tCGCTTAAAACCGCGTTAACGCAGCAGCAA‑GTCTTGATGCGGCGCTGATTGATGCGCCGTTAGCAGTCGATGCACAAACGCCTCTTAGCGAGTTGCTCTCTCATGTCGGACAGGCACCCTGTGCGGTGCCCGTGGTCGACGAGGACCAAc                           <  1:1286184/150‑1 (MQ=255)

                                                                                                                       cGCTTAAAACCGCGTTAACGCAGCAGCAA‑GTCTTGATGCGGCGCTGATTGATGCGCCGTTAGCAGTCGATGCACAAACGCCTCTTAGCGAGTTGCTCTCTCATGTCGGACAGGCACCCTGTGCGGTGCCCGTGGTCGACGAGGACCAACa                          <  1:3700802/150‑1 (MQ=255)

                                                                                                                       cGCTTAAAACCGCGTTAACGCAGCAGCAA‑GTCTTGATGCGGCGCTGATTGATGCGCCGTTAGCAGTCGATGCACAAACGCCTCTTAGCGAGTTGCTCTCTCATGTCGGACAGGCACCCTGTGCGGTGCCCGTGGTCGACGAGGACCAACa                          <  1:821243/150‑1 (MQ=255)

                                                                                                                          ttAAAACCGCGTTAACGCAGCAGCAA‑GTCTTGATGCGGCGCTGATTGATGCGCCGTTAGCAGTCGATGCACAAACGCCTCTTAGCGAGTTGCTCTCTCATGTCGGACAGGCACCCTGTGCGGTGCCCGTGGTCGACGAGGACCAACAGTa                       <  2:1064535/150‑1 (MQ=255)

                                                                                                                           tAAAACCGCGTTAACGCAGCAGCAA‑GTCTTGATGCGGCGCTGATTGATGCGCCGTTAGCAGTCGATGCACAAACGCCTCTTAGCGAGTTGCTCTCTCATGTCGGACAGGCACCCTGTGCGGTGCCCGTGGTCGACGAGGACCAACAGTAt                      <  1:2080422/150‑1 (MQ=255)

                                                                                                                            aaaaCCGCGTTAACGCAGCAGCAA‑GTCTTGATGCGGCGCTGATTGATGCGCCGTTAGCAGTCGATGCACAAACGCCTCTTAGCGAGTTGCTCTCTCATGTCGGACAGGCACCCTGTGCGGTGCCCGTGGTCGACGAGGACCAACAGTATg                     <  1:66054/150‑1 (MQ=255)

                                                                                                                            aaaaCCGCGTTAACGCAGCAGCAA‑GTCTTGATGCGGCGCTGATTGATGCGCCGTTAGCAGTCGATGCACAAACGCCTCTTAGCGAGTTGCTCTCTCATGTCGGACAGGCACCCTGTGCGGTGCCCGTGGTCGACGAGGACCAACAGTATg                     <  2:3611275/150‑1 (MQ=255)

                                                                                                                              aaCCGCGTTAACGCAGCAGCAA‑GTCTTGATGCGGCGCTGATTGATGCGCCGTTAGCAGTCGATGCACAAACGCCTCTTAGCGAGTTGCTCTCTCATGTCGGACAGGCACCCTGTGCGGTGCCCGTGGTCGACGAGGACCAACAGTATGTc                   <  2:2841146/150‑1 (MQ=255)

                                                                                                                                 cgcgTTAACGCAGCAGCAA‑GTCTTGATGCGGCGCTGATTGATGCGCCGTTAGCAGTCGATGCACAAACGCCTCTTAGCGAGTTGCTCTCTCATGTCGGACAGGCACCCTGTGCGGTGCCCGTGGTCGACGAGGACCAACAGTATGTCGGc                <  2:1544868/150‑1 (MQ=255)

                                                                                                                                 cgcgTTAACGCAGCAGCAA‑GTCTTGATGCGGCGCTGATTGATGCGCCGTTAGCAGTCGATGCACAAACGCCTCTTAGCGAGTTGCTCTCTCATGTCGGACAGGCACCCTGTGCGGTGCCCGTGGTCGACGAGGACCAACAGCATGACgga                >  2:1498845/1‑149 (MQ=255)

                                                                                                                                  gcgTTAACGCAGCAGCAA‑GTCTTGATGCGGCGCTGATTGATGCGCCGTTAGCAGTCGATGCACAAACGCCTCTTAGCGAGTTGCTCTCTCATGTCGGACAGGCACCCTGTGCGGTGCCCGTGGTCGACGAGGACCAACAGTATGTCGGca               >  2:2651126/1‑150 (MQ=255)

                                                                                                                                   cgTTAACGCAGCAGCAA‑GTCTTGATGCGGCGCTGATTGATGCGCCGTTAGCAGTCGATGCACAAACGCCTCTTAGCGAGTTGCTCTCTCATGTCGGACAGGCACCCTGTGCGGTGCCCGTGGTCGACGAGGACCAACAGTATGTCGGcat              <  1:1314575/150‑1 (MQ=255)

                                                                                                                                    atcagcgccgcAGCAA‑GTCTTGATGCGGCGCTGATTGATGCGCCGTTAGCAGTCGATGCACAAACGCCTCTTAGCGAGTTGCTCTCTCATGTCGGACAGGCACCCTGTGCGGTGCCCGTGGTCGACGAGGACCAACAGTATGTCGGcatc             >  2:2980063/6‑150 (MQ=255)

                                                                                                                                     ttAACGCAGCAGCAA‑GTCTTGATGCGGCGCTGATTGATGCGCCGTTAGCAGTCGATGCACAAACGCCTCTTAGCGAGTTGCTCTCTCATGTCGGACAGGCACCCTGTGCGGTGCCCGTGGTCGACGAGGACCAACAGTATGTCGGcatca            <  1:1963235/150‑1 (MQ=255)

                                                                                                                                      tAACGCAGCAGCAA‑GTCTTGATGCGGCGCTGATTGATGCGCCGTTAGCAGTCGATGCACAAACGCCTCTTAGCGAGTTGCTCTCTCATGTCGGACAGGCACCCTGTGCGGTGCCCGTGGTCGACGAGGACCAACAGTATGTCGGcatcat           <  1:2704743/150‑1 (MQ=255)

                                                                                                                                        aCGCAGCAGCAA‑GTCTTGATGCGGCGCTGATTGATGCGCCGTTAGCAGTCGATGCACAAACGCCTCTTAGCGAGTTGCTCTCTCATGTCGGACAGGCACCCTGTGCGGTGCCCGTGGTCGACGAGGACCAACAGTATGTCGGCATCAttt         <  1:4445182/150‑1 (MQ=255)

                                                                                                                                        aCGCAGCAGCAA‑GTCTTGATGCGGCGCTGATTGATGCGCCGTTAGCAGTCGATGCACAAACGCCTCTTAGCGAGTTGCTCTCTCATGTCGGACAGGCACCCTGTGCGGTGCCCGTGGTCGACGAGGACCAACAGTATGTCGGCATCAttt         >  1:3784871/1‑150 (MQ=255)

                                                                                                                                        aCGCAGCAGCAA‑GTCTTGATGCGGCGCTGATTGATGCGCCGTTAGCAGTCGATGCACAAACGCCTCTTAGCGAGTTGCTCTCTCATGTCGGACAGGCACCCTGTGCGGTGCCCGTGGTCGACGAGGACCAACAGTATGTCGGCATCAttt         <  1:1394020/150‑1 (MQ=255)

                                                                                                                                         cGCAGCAGCAA‑GTCTTGATGCGGCGCTGATTGATGCGCCGTTAGCAGTCGATGCACAAACGCCTCTTAGCGAGTTGCTCTCTCATGTCGGACAGGCACCCTGTGCGGGGCCCGTGGTCGACGAGGACCAACAGTATGTCGGCATCATTTc        <  2:1223203/150‑1 (MQ=255)

                                                                                                                                          gcagcagcaA‑GTCTTGATGCGGCGCTGATTGATGCGCCGTTAGCAGTCGATGCACAAACGCCTCTTAGCGAGTTGCTCTCTCATGTCGGACAGGCACCCTGTGCGGTGCCCGTGGTCGACGAGGACCAACAGTATGTCGGCATCATTTCg       <  2:231655/150‑1 (MQ=255)

                                                                                                                                          gcagcagcaA‑GTCTTGATGCGGCGCTGATTGATGCGCCGTTAGCAGTCGATGCACAAACGCCTCTTAGCGAGTTGCTCTCTCATGTCGGACAGGCACCCTGTGCGGTGCCCGTGGTCGACGAGGACCAACAGTATGTCGGCATCATTTCg       <  1:328303/150‑1 (MQ=255)

                                                                                                                                            agcagcaA‑GTCTTGATGCGGCGCTGATTGATGCGCCGTTAGCAGTCGATGCACAAACGCCTCTTAGCGAGTTGCTCTCTCATGTCGGACAGGCACCCTGTGCGGTGCCCGTGGTCGACGAGGACCAACAGTATGTCGGCATCATTTCGaa     >  2:2143958/1‑150 (MQ=255)

TCGTAAAACCCCTGGCTTCGGCCCACGTTCGGCACTGAAATTATTGCAGGATGAAGATCGCGAATATGGCTACGTTATCGAACGCGGTAATAAGTTTGTCGGCGCAGTCTCCATCGATTCGCTTAAAACCGCGTTAACGCAGCAGCAAGGTCTTGATGCGGCGCTGATTGATGCGCCGTTAGCAGTCGATGCACAAACGCCTCTTAGCGAGTTGCTCTCTCATGTCGGACAGGCACCCTGTGCGGTGCCCGTGGTCGACGAGGACCAACAGTATGTCGGCATCATTTCGAAAGG  >  AP012306/2316213‑2316506

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`