Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP012306202,4170TG28.5% 598.8 / 39.0 271V409G (GTA→GGA) tilStRNA(Ile)‑lysidine synthetase
Reads supporting (aligned to +/- strand):  ref base T (77/116);  new base G (77/0);  total (155/116)
Fisher's exact test for biased strand distribution p-value = 2.47e-24
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 324 total aligned reads displayed.

TTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGATTCGCTTACCACCACCGTACCG  >  AP012306/202268‑202566
                                                                                                                                                     |                                                                                                                                                     
  tCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTAt                                                                                                                                                     <  2:1203915/150‑1 (MQ=255)
    aaaGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGGAttt                                                                                                                                                   >  1:351691/1‑150 (MQ=255)
    aaaGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGGAttt                                                                                                                                                   >  1:2649750/1‑150 (MQ=255)
       gcgcCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTtgtg                                                                                                                                                <  1:1414849/150‑1 (MQ=255)
        cgcCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGGATTtttgg                                                                                                                                               >  1:3336248/1‑149 (MQ=255)
           cAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTTCGGCGGCAGGGGGAttttgtgccc                                                                                                                                            >  1:3459951/1‑143 (MQ=255)
            aGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCa                                                                                                                                           <  1:488557/150‑1 (MQ=255)
              gATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCTGCGGCAGGGGGATTTGTGACggcag                                                                                                                                         >  1:3574529/1‑146 (MQ=255)
               aTTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCaaga                                                                                                                                        <  1:3924066/150‑1 (MQ=255)
                ttGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCaagaa                                                                                                                                       <  2:777838/150‑1 (MQ=255)
                ttGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGGGTTTGTGACGCcagaa                                                                                                                                       >  2:3083536/1‑150 (MQ=255)
                 tgctgcATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCCCTGCTGTTTTATGGCGAAACCCTGATTGCGGCGGGAGGGGGATTTGTGACGCaagaag                                                                                                                                      >  2:87061/1‑150 (MQ=255)
                 tgctgcATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGGATTTGTGACGCaagaag                                                                                                                                      >  1:450416/1‑150 (MQ=255)
                  gctgcATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGGCGGGGGGTTTTTGACGGcagaaag                                                                                                                                     >  2:3476950/1‑148 (MQ=255)
                  gctgcATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAgg                                                                                                                                     <  2:2451643/150‑1 (MQ=255)
                   ctgcATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCCGGGGGATTTGTGACGCAAGAAggg                                                                                                                                    >  1:3006775/1‑149 (MQ=255)
                   ctgcATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGGATTTTTGAGGCaaagaggg                                                                                                                                    >  2:2980564/1‑144 (MQ=255)
                      cATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAATAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGGATTTGTGACGCAAGAAGGtgtg                                                                                                                                 >  1:404295/1‑150 (MQ=255)
                      cATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAAAGCTGATTGCGGCGGCAGGGGGATTTGTGAAGCACGAAGgggtt                                                                                                                                 >  1:4124494/1‑149 (MQ=255)
                        tatTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGGGTTTTTGACGCCAGAAGGGGTggg                                                                                                                               >  2:3424027/1‑149 (MQ=255)
                        tatTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGGATTTTTGACGCAAGAAGGGGTGGc                                                                                                                               >  1:2120660/1‑150 (MQ=255)
                         atTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGGATTTGTGACGCCAGGAGGGGTgggt                                                                                                                              >  1:3879275/1‑148 (MQ=255)
                               gggCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGt                                                                                                                        <  1:2362073/150‑1 (MQ=255)
                                    tAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGGATTTTTGACGCCAGAAAGGGTGGGTGGAGGGGAGaa                                                                                                                   >  2:2508058/1‑150 (MQ=255)
                                     aaCGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAAt                                                                                                                  <  2:2248770/150‑1 (MQ=255)
                                      aCGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATg                                                                                                                 <  2:4169468/150‑1 (MQ=255)
                                      aCGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCAGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGGATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATg                                                                                                                 >  1:446184/1‑150 (MQ=255)
                                          cggACGTAAGCTAAAGAAAATCTGGCAAGTGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGGATTTGTGACGCAAGAAGGGGTGGCTGAAGGGGAGAATGGCGt                                                                                                             >  1:422779/1‑150 (MQ=255)
                                           ggACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTa                                                                                                            >  1:4259502/1‑150 (MQ=255)
                                           ggACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGGATTTGTGACGCAAGAAGGTGTGGCTGAAGGGGAGAATGGCGTa                                                                                                            >  2:1034421/1‑150 (MQ=255)
                                               gTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGtt                                                                                                        >  1:699388/1‑150 (MQ=255)
                                               gTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGtt                                                                                                        <  1:799480/150‑1 (MQ=255)
                                                 aaGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGtttt                                                                                                      >  1:3716549/1‑150 (MQ=255)
                                                  aGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTg                                                                                                     <  2:2921951/150‑1 (MQ=255)
                                                   gCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGGGTGGCTGAAGGGGGGAAAGGGGGAAGtttttt                                                                                                    >  2:4114404/1‑148 (MQ=255)
                                                     tAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCt                                                                                                  <  2:4202351/150‑1 (MQ=255)
                                                     tAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCt                                                                                                  >  1:1260242/1‑150 (MQ=255)
                                                     tAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGGATTTTTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCt                                                                                                  >  2:3323465/1‑150 (MQ=255)
                                                      aaaGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGGATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTg                                                                                                 >  2:3256118/1‑150 (MQ=255)
                                                      aaaGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGGATTTGTGACGCAAGAAGGTGTGGCTGAAGGGGAGAATGGCGGAAGGTTTGTCTg                                                                                                 >  2:1806191/1‑150 (MQ=255)
                                                         gAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCa                                                                                              >  1:573113/1‑150 (MQ=255)
                                                         gAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGGATTTGTGACGCAAGAAGGTGTGGCTGAAGGGGAGAATGGCGTAAGTTTTGTCTGGCa                                                                                              >  1:3072432/1‑150 (MQ=255)
                                                          aaaaTCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAg                                                                                             >  1:2897146/1‑150 (MQ=255)
                                                          aaaaTCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGGATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAg                                                                                             >  1:225815/1‑150 (MQ=255)
                                                           aaaTCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGGATTTGTGACGCAAGAAGGGGTGGCTGAAGGGGAGAATGGCGTAAGTTTTGTCTGGCAGa                                                                                            >  2:673831/1‑150 (MQ=255)
                                                            aaTCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTGCGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGaa                                                                                           <  2:1741152/150‑1 (MQ=255)
                                                            aaTCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGGGACGCAAGAAAGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGaa                                                                                           >  1:1968947/1‑150 (MQ=255)
                                                             aTCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGaaa                                                                                          <  1:2310314/150‑1 (MQ=255)
                                                             aTCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGaaa                                                                                          <  2:2366452/150‑1 (MQ=255)
                                                                 ggCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGc                                                                                      <  2:2120198/150‑1 (MQ=255)
                                                                   cAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGAATTTGGTACGCGAGAAGGGTAGGCCGAACGGGAGGATGGCGTAAGTTTTGTCGGGAGGAAAACGCtt                                                                                    >  2:1396481/1‑150 (MQ=255)
                                                                    aaGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGGATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGCTTTGTCTGGCAGAAAACGCTTa                                                                                   >  1:1031248/1‑150 (MQ=255)
                                                                        gCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGGATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGGGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTa                                                                               >  2:1132325/1‑150 (MQ=255)
                                                                         cTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTaa                                                                              >  1:1096130/1‑150 (MQ=255)
                                                                              cGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGGATTTTTGACGCAAGAAGGTGTGGCTGGAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGaa                                                                         >  2:2303237/1‑150 (MQ=255)
                                                                                 gccgccGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGc                                                                      <  2:1473801/150‑1 (MQ=255)
                                                                                  ccgccgTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGcc                                                                     <  1:1019376/150‑1 (MQ=255)
                                                                                    gccgTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCgg                                                                   <  2:3574525/150‑1 (MQ=255)
                                                                                    gccgTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGGATTTGTGACGCAAGAAGGTGTGGCTGAAGGGGAGAATGGCGGAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGGGAAAGCCgg                                                                   >  2:374015/1‑150 (MQ=255)
                                                                                     ccgTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGGATTTGTGACGCCAGAAGGTGTGGCTGGAGGTGAGAATGGCGTAAGGTTTTTCTGGCAGAAAAAGCTTTGTTAAATGAAAACCGGa                                                                  >  2:3914388/1‑150 (MQ=255)
                                                                                     ccgTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGGATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGa                                                                  >  1:190646/1‑150 (MQ=255)
                                                                                          gCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGa                                                             <  1:240771/150‑1 (MQ=255)
                                                                                          gCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGa                                                             >  1:4334104/1‑150 (MQ=255)
                                                                                          gCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGGGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGa                                                             >  2:319260/1‑150 (MQ=255)
                                                                                            tACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACg                                                           <  2:1978679/150‑1 (MQ=255)
                                                                                              cGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCa                                                         <  1:3614416/150‑1 (MQ=255)
                                                                                              cGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCa                                                         >  1:4156484/1‑150 (MQ=255)
                                                                                              cGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGGATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCa                                                         >  2:366036/1‑150 (MQ=255)
                                                                                               gTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCAt                                                        <  1:1854512/150‑1 (MQ=255)
                                                                                               gTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGGATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCAt                                                        >  1:991104/1‑150 (MQ=255)
                                                                                                tGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGGATTTGTGACGCAAGAAGGGGGGGCTGGAGGGGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATc                                                       >  2:567267/1‑150 (MQ=255)
                                                                                                   caccacGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCaaa                                                    <  1:3391727/150‑1 (MQ=255)
                                                                                                   caccacGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGGATTTGTGACGCAAGAAGGTGTGGCTGAAGGGGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCaaa                                                    >  2:1903019/1‑150 (MQ=255)
                                                                                                    accacGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAAc                                                   <  1:2933100/150‑1 (MQ=255)
                                                                                                     ccacGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACg                                                  >  1:3991805/1‑150 (MQ=255)
                                                                                                          ccACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCa                                             <  1:1537688/150‑1 (MQ=255)
                                                                                                           cACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGGGAGAATGGCGGAAGTTTTTTCTGGCAGAAAAAGCTTAGTTAAGTGAAAACCGGATAAGACGCATCAAACGTCGCAt                                            >  2:2048900/1‑150 (MQ=255)
                                                                                                             ctgctgTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATcc                                          >  1:2084647/1‑150 (MQ=255)
                                                                                                              tgctgTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCg                                         <  1:3676355/150‑1 (MQ=255)
                                                                                                                ctgTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGc                                       <  1:3173261/150‑1 (MQ=255)
                                                                                                                ctgTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGGATTTGTGACGCAAGAAGGTGTGGCTGAAGGGGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGc                                       >  2:1869602/1‑150 (MQ=255)
                                                                                                                      tATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGt                                 >  1:2826924/1‑150 (MQ=255)
                                                                                                                         ggCGAAACGCTGATTGCGGCGGCAGGGGGATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCaa                              >  2:378705/1‑150 (MQ=255)
                                                                                                                           cGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATc                            <  2:2662286/150‑1 (MQ=255)
                                                                                                                            gAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCa                           >  1:3274933/1‑150 (MQ=255)
                                                                                                                              aaCGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAgg                         >  2:1759603/1‑150 (MQ=255)
                                                                                                                              aaCGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAgg                         >  1:2549278/1‑150 (MQ=255)
                                                                                                                                   tGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGATTCg                    >  1:1201134/1‑150 (MQ=255)
                                                                                                                                     aTTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGATTCGCt                  <  1:1759603/150‑1 (MQ=255)
                                                                                                                                     aTTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGATTCGCt                  <  1:2750290/150‑1 (MQ=255)
                                                                                                                                     aTTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGATTCGCt                  <  1:1491935/150‑1 (MQ=255)
                                                                                                                                     aTTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGATTCGCt                  >  1:3469543/1‑150 (MQ=255)
                                                                                                                                      ttGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGATTCGCtt                 <  1:919002/150‑1 (MQ=255)
                                                                                                                                           gcggcAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGATTCGCTTaccac            <  2:2164025/150‑1 (MQ=255)
                                                                                                                                            cggcAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGATTCGCTTaccacc           >  2:1264687/1‑150 (MQ=255)
                                                                                                                                               cAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGATTCGCTTaccaccacc        >  2:1611345/1‑150 (MQ=255)
                                                                                                                                                aGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGATTCGCTTACCACCACCg       >  1:1187900/1‑150 (MQ=255)
                                                                                                                                                aGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGATTCGCTTACCACCACCg       >  2:2862901/1‑150 (MQ=255)
                                                                                                                                                aGGGGTATTTGTAACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGATTCGCTTACCACCACCg       >  2:4547260/1‑150 (MQ=255)
                                                                                                                                                     tATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGATTCGCTTACCACCACCGTACCg  >  2:4370154/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGATTCGCTTACCACCACCGTACCG  >  AP012306/202268‑202566

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑