Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP012306472,8680TG22.5% 547.6 / 11.1 200D435A (GAC→GCC) cusSsensory histidine kinase in two‑component regulatory system with CusR, senses copper ions
Reads supporting (aligned to +/- strand):  ref base T (39/116);  new base G (45/0);  total (84/116)
Fisher's exact test for biased strand distribution p-value = 9.87e-22
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 255 total aligned reads displayed.

TGCTGAAGGATTAAGCGGGTAATGTGATAACAAACCTTGTCCCCCGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGA‑GGGGTCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGCGCAATGGGCGT‑ACCGGGGTTTTCGACGATAACTTGCACCAGGTGATCGACCGTCTGACAGCGCACTACAATTGTCTCTCCGGTTGGCGTATAACGCAGGGCGTT  >  AP012306/472719‑473016
                                                                                                                                                      |                                                                                                                                                     
  cTGAAGGATTAAGCGGGTAATGTGATAACAAACCTTGTCCCCCGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGA‑GGGGTCa                                                                                                                                                     <  1:3686046/150‑1 (MQ=255)
   tGAAGGATTAAGCGGGTAATGTGATAACAAACCTTGTCCCCCGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGA‑GGGGTCaa                                                                                                                                                    <  1:326121/150‑1 (MQ=255)
    gAAGGATTAAGCGGGTAATGTGATAACAAACCTTGTCCCCCGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGA‑GGGGTCAAc                                                                                                                                                   <  1:246036/150‑1 (MQ=255)
     aaGGATTAAGCGGGTAATGTGATAACAAACCTTGTCCCCCGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGCTTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGA‑GGGGTCAAcg                                                                                                                                                  <  2:368385/150‑1 (MQ=255)
     aaGGATTAAGCGGGTAATGTGATAACAAACCTTGTCCCCCGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGA‑GGGGTCAAcg                                                                                                                                                  >  1:1602251/1‑150 (MQ=255)
      aGGATTAAGCGGGTAATGTGATAACAAACCTTGTCCCCCGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGG‑GGGGGCAAcgc                                                                                                                                                 >  2:2353588/1‑150 (MQ=255)
      aGGATTAAGCGGGTAATGTGATAACAAACCTTGTCCCCCGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGA‑GGGGTCAAcgc                                                                                                                                                 <  2:906494/150‑1 (MQ=255)
        gATTAAGCGGGTAATGTGATAACAAACCTTGTCCCCCGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGA‑GGGGGCAACGCGa                                                                                                                                               >  2:4153417/1‑150 (MQ=255)
           tAAGCGGGTAATGTGATAACAAACCTTGTCCCCCGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGA‑GGGGGCAACGCGATAg                                                                                                                                            >  2:1806227/1‑150 (MQ=255)
            aaGCGGGTAATGTGATAACAAACCTTGTCCCCCGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGG‑GGGGTCCACGCGATAGa                                                                                                                                           >  2:1506152/1‑150 (MQ=255)
            aaGCGGGTAATGTGATAACAAACCTTGTCCCCCGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGA‑GGGGTCAACGCGATAGa                                                                                                                                           <  1:3324999/150‑1 (MQ=255)
             aGCGGGTAATGTGATAACAAACCTTGTCCCCCGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGG‑GGGGGCAACGCGGTaaaa                                                                                                                                          >  2:2044794/1‑150 (MQ=255)
             aGCGGGTAATGTGATAACAAACCTTGTCCCCCGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGA‑GGGGTCAACGCGATAGaa                                                                                                                                          <  1:934041/150‑1 (MQ=255)
              gCGGGTAATGTGATAACAAACCTTGTCCCCCGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGA‑GGGGTCAACGCGATAGaaa                                                                                                                                         <  2:4078413/150‑1 (MQ=255)
               cGGGTAATGTGATAACAAACCTTGTCCCCCGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGA‑GGGGTCAACGCGATAGAAAc                                                                                                                                        >  2:2430798/1‑150 (MQ=255)
                gggTAATGTGATAACAAACCTTGTCCCCCGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGA‑GGGGTCAACCCGATAGAAACg                                                                                                                                       >  1:3872950/1‑150 (MQ=255)
                gggTAATGTGATAACAAACCTTGTCCCCCGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGA‑GGGGGCAACGCGATAGAAACg                                                                                                                                       >  1:1145926/1‑150 (MQ=255)
                  gTAATGTGATAACAAACCTTGTCCCCCGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGG‑GGGGGCAACCCGATAAAAACggc                                                                                                                                     >  2:3455728/1‑149 (MQ=255)
                   tAATGTGATAACAAACCTTGTCCCCCGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGA‑GGGGTCAACGCGATAGAAACGGTc                                                                                                                                    <  2:3997696/150‑1 (MQ=255)
                   tAATGTGATAACAAACCTTGTCCCCCGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGA‑GGGGGCAACGCGATAGAAACGGTc                                                                                                                                    >  1:2969167/1‑150 (MQ=255)
                    aaTGTGATAACAAACCTTGTCCCCCGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGA‑GGGGTCAACGCGATAGAAACGGTCa                                                                                                                                   >  2:837547/1‑150 (MQ=255)
                        tgATAACAAACCTTGTCCCCCGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGA‑GGGGTCAACGCGATAGAAACGGTCAAACa                                                                                                                               <  1:1989621/150‑1 (MQ=255)
                           tAACAAACCTTGTCCCCCGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGA‑GGGGTCAACGCGATAGAAACGGTCAAACAATc                                                                                                                            <  1:494413/150‑1 (MQ=255)
                           tAACAAACCTTGTCCCCCGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGA‑GGGGTCAACGCGATAGAAACGGTCAAACAATc                                                                                                                            <  1:3846842/150‑1 (MQ=255)
                            aaCAAACCTTGTCCCCCGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCTCTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGA‑GGGGGCAACGCGATAGAAACGGTCAAACAATcg                                                                                                                           >  1:2427177/1‑150 (MQ=255)
                            aaCAAACCTTGTCCCCCGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGA‑GGGGTCAACGCGATAGAAACGGTCAAACAATcg                                                                                                                           <  2:869234/150‑1 (MQ=255)
                               aaaCCTTGTCCCCCGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGG‑GGGGTCCACGCGGTAGAAACGGGCAAACAATCGCgg                                                                                                                        >  1:3115251/1‑150 (MQ=255)
                                 aCCTTGTCCCCCGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGA‑GGGGTCAACGCGATAGAAACGGTCAAACAATCGCGGTa                                                                                                                      <  1:1347231/150‑1 (MQ=255)
                                  ccTTGTCCCCCGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGA‑GGGGGCAACGCGATAGAAACGGTCAAAAAAACGCGGTaa                                                                                                                     >  2:1178441/1‑150 (MQ=255)
                                    ttGTCCCCCGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGA‑GGGGGCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGt                                                                                                                   >  1:2883707/1‑150 (MQ=255)
                                     tGTCCCCCGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGTCAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGA‑GGGGGCAACGCGATAGAAACGGTCAAACAATCGCGGGAAGTg                                                                                                                  >  1:278126/1‑150 (MQ=255)
                                        cccccGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGA‑GGGGTCAACGCGATAGAAACGGGCACACAATCGCGGTAAGTGCTc                                                                                                               >  1:3730420/1‑150 (MQ=255)
                                         ccccGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGG‑GGGGGCAACGCGATAGAAACGGGCAAACAAACGCGGGAAGTGCTCg                                                                                                              >  2:1874431/1‑150 (MQ=255)
                                           ccGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGA‑GGGGTCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCggg                                                                                                            <  1:305907/150‑1 (MQ=255)
                                            cgcgCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGG‑GGGGGCAACGCGATAGAAACGGTCAAACAATCGCGGGATGTGCTCGGgc                                                                                                           >  1:4200411/1‑150 (MQ=255)
                                            cgcgCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGA‑GGGGTCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGgc                                                                                                           >  2:1515764/1‑150 (MQ=255)
                                            cgcgCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGA‑GGGGTCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGgc                                                                                                           <  2:2403388/150‑1 (MQ=255)
                                               gcATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGA‑GGGGTCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGCGCa                                                                                                        <  1:2166876/150‑1 (MQ=255)
                                               gcATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGA‑GGGGGCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCCGGCGCa                                                                                                        >  1:2279423/1‑150 (MQ=255)
                                                  tCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGA‑GGGGTCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGCGCAATg                                                                                                     <  1:2207042/150‑1 (MQ=255)
                                                    cGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGA‑GGGGTCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGCGCAATggg                                                                                                   >  1:772036/1‑150 (MQ=255)
                                                     gACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGG‑GGGGGCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGCGCAATGGGc                                                                                                  >  1:172360/1‑150 (MQ=255)
                                                       cGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGA‑GGGGTCCACGCGGTAGAAACGGCACAAAAAACTCGGTAAGAGCTTGGGCCCAATGGGGGt                                                                                                >  2:2975571/1‑150 (MQ=255)
                                                        gTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGA‑GGGGGCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGCGCAATGGGCgga                                                                                               >  2:1137706/1‑148 (MQ=255)
                                                         ttACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGA‑GGGGTCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGCGCAATGGGCGT‑Ac                                                                                             <  1:3544404/150‑1 (MQ=255)
                                                           aCCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGA‑GGGGTCAACGCGATAGAAACGGTCAAACAAACGCGGGAAGTGCTCGGGCGCAATGGGCggaacg                                                                                            >  2:3106310/1‑145 (MQ=255)
                                                             cGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGG‑GGGGGCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGCGCAATGGGCGT‑ACCggg                                                                                         >  1:1296209/1‑150 (MQ=255)
                                                               cAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGA‑GGGGTCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGCGCAATGGGCGT‑ACCGGGGt                                                                                       <  1:2364167/150‑1 (MQ=255)
                                                                 aCCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGA‑GGGGTCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGCGCAATGGGCGT‑ACCGGGGttt                                                                                     <  2:2473443/150‑1 (MQ=255)
                                                                    gTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGA‑GGGGGCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGCGCAATGGGCGT‑ACCGGGGGTTTcg                                                                                  >  1:4116590/1‑150 (MQ=255)
                                                                      gCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGA‑GGGGTTAACGCGATAGAAACGGTCAAAAAATCGCGGTAAGTGGTTGGGGGCAATGGGGGT‑ACCGGGGTGTTcgac                                                                                >  2:683769/1‑150 (MQ=255)
                                                                      gCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGA‑GGGGTCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGTTCGGGCGCAATGGGCGT‑ACCGGGGTTTTcgac                                                                                <  2:2375417/150‑1 (MQ=255)
                                                                      gCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGA‑GGGGTCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGCGCAATGGGCGT‑ACCGGGGTTTTcgac                                                                                <  1:436735/150‑1 (MQ=255)
                                                                      gCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGA‑GGGGTCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGCGCAATGGGCGT‑ACCCGGGGTTTcgac                                                                                >  1:1306550/1‑150 (MQ=255)
                                                                           tATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGA‑GGGGTCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGCGCAATGGGCGT‑ACCGGGGTTTTCGACGATaa                                                                           <  1:876298/150‑1 (MQ=255)
                                                                            aTGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGGCCAACCCG‑TAGAAAACGTCAAACAAAACCGGGAAGTGGTCGGGCGCCATGGGGGT‑ACCGGGGGTTTTGACCATAAc                                                                          >  2:1390078/1‑150 (MQ=255)
                                                                                   acaacaATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGA‑GGGGTCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGCGCAATGGGCGT‑ACCGGGGTTTTCGACGATAACTTGCAcc                                                                   >  1:1510753/1‑150 (MQ=255)
                                                                                   acaacaATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGA‑GGGGGCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGCGCAATGGGCGT‑ACCGGGGGTTTCGACGATAACTTGCAcc                                                                   >  1:2668779/1‑150 (MQ=255)
                                                                                          tCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGA‑GGGGGCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGCGCAAAGGGCGT‑ACCGGGGGTTTCGACGATAACTTGCACCAGGGGAt                                                            >  2:3864627/1‑150 (MQ=255)
                                                                                           cGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGA‑GGGGTCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGCGCAATGGGCGT‑ACCGGGGTTTTCGACGATAACTTGCACCAGGTGATc                                                           <  1:678731/150‑1 (MQ=255)
                                                                                              tttCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGG‑GGGGGCAACGCGATAGAAACGGTCAAAAAAACGCGGTAAGTGCTCCGGCGCCAAGGGCGG‑AACGGGGGTTTCGACGATAACTTGCACCCAGGGATCGAc                                                        >  2:4051701/1‑150 (MQ=255)
                                                                                               ttCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGA‑GGGGTCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGCGCAATGGGCGT‑ACCGGGGTTTTCGACGATAACTTGCACCAGGTGATCGAcc                                                       <  1:2730055/150‑1 (MQ=255)
                                                                                                tCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGA‑GGGGGCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGCGCAATGGGCGT‑ACCGGGGGTTTCGACGATAAATTGCACCAGGTGATCGACCg                                                      >  1:1521484/1‑150 (MQ=255)
                                                                                                  aCTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGA‑GGGGTCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGCGCAATGGGCGT‑ACCGGGGTTTTCGACGATAACTTGCACCAGGTGATCGACCGTc                                                    <  2:19636/150‑1 (MQ=255)
                                                                                                  aCTATCGCCAGGCCAATACCGCTACCTTCACCTTCTCGCTGGCGGGA‑GGGGGCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGCGCCATGGGCGG‑ACCGGGGGTTTCGACGATAACTTGCACCAGGTGGTCGACCGTc                                                    >  2:126538/1‑150 (MQ=255)
                                                                                                    tATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGG‑GGGGTCAACGCGATAGAAACGGTCAAACAATCGCGGGAAGTGCTCGGGCGCAAAGGGCGT‑ACCGGGGGTTTCGACGATAACTTTCACCAGGTGATCGACCCTCTg                                                  >  2:259523/1‑150 (MQ=255)
                                                                                                         ccAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGG‑GGGGGCAACGCGATAGAAACGGGCAAACAAACGCGGGAAATGCTCGGGGGCAATGGGGGG‑ACCGGGGGTTTCGACGATAACTTGCACCAGGGGATCGACCGTCTGACAgc                                             >  2:961202/1‑150 (MQ=255)
                                                                                                            ggCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGA‑GGGGTCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGCGCAATGGGCGT‑ACCGGGGGTTTCGACGATAACTTGCACCAGGTGATCGACCGTCTGACAGCGCa                                          >  1:3621793/1‑150 (MQ=255)
                                                                                                            ggCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGA‑GGGGGCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGCGCAATGGGCGT‑ACCGGGGGTTTCGACGATAACTTGCACCAGGGGATCGACCGTCTGACAGCGCa                                          >  2:3897532/1‑150 (MQ=255)
                                                                                                             gCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGG‑GGGGGCAACGCGATAAAAAAGGTCAAACAATCGCGGGAAGTGCTCGGGCGCAATGGGCGG‑ACCGGGGTTTTTGACGATAACTTGCACCAGGTGATCGACCGTCTGACAGCGCAc                                         >  2:1450173/1‑150 (MQ=255)
                                                                                                             gCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGA‑GGGGTCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGCGCAATGGGCGT‑ACCGGGGTTTTCGACGATAACTTGCACCAGGTGATCGACCGTCTGACAGCGCAc                                         <  1:392923/150‑1 (MQ=255)
                                                                                                              ccAATACCGCTACCTTCACCTTTTCGCTGGCGGGG‑GGGGGCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGCGCAATGGGCGT‑ACCGGGGGTTTCGACGATAACTTGCACCAGGGGATCGACCGTCTGACAGCGCACt                                        >  1:1274108/1‑150 (MQ=255)
                                                                                                              ccAATACCGCTACCTTCACCTTTTCGCTGGCGGGA‑GGGGGCAACGCGGTAAAAAAGGGCAAACAAACGCGGGAAGTGGTCGGGCGCAAAGGGGGG‑ACCGGGGGTTTCGACGATAACATTTACCCGGGGATCGACCGTCTGGCCACGCACt                                        >  2:3187147/1‑150 (MQ=255)
                                                                                                               cAATACCGCTACCTTCACCTTTTCGCTGGCGGGA‑GGGGTCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGCGCAATGGGCGT‑ACCGGGGTTTTCGACGATAACTTGCACCAGGTGATCGACCGTCTGACAGCGCACTa                                       <  1:3988510/150‑1 (MQ=255)
                                                                                                               cAATACCGCTACCTTCACCTTTTCGCTGGCGGGA‑GGGGGCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGCGCAATGGGCGT‑ACCGGGGGTTTCGACGATAACTTGCACCAGGTGATCGACCGTCTGACAGCGCACTa                                       >  2:2248160/1‑150 (MQ=255)
                                                                                                                aaTACCGCTACCTTCACCTTTTCGCTGGCGGGA‑GGGGGCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCCGGCGCAATGGGCGG‑ACCGGGGGTTTCGACGGAAACTTGCACCCGGTGATCGACCGTCTGACAGCGCACTAc                                      >  2:2500450/1‑150 (MQ=255)
                                                                                                                  tACCGCTACCTTCACCTTTTCGCTGGCGGGA‑GGGGGCAACGCGATAGAAACGGTCAAACAATCGCGGGAAGTGCTCGGGCGCAATGGGCGT‑ACCGGGGTTTTCGACGATAACTTGCACCAGGTGATCGACCGTCTGACAGCGCACTACaa                                    >  1:1592460/1‑150 (MQ=255)
                                                                                                                    ccGCTACCTTTACCTTTTCGCTGGCGGGA‑GGGGTCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGCGCAATGGGCGT‑ACCGGGGTTTTCGACGATAACTTGCACCAGGTGATCGACCGTCTGACAGCGCACTACAAtt                                  <  2:286286/150‑1 (MQ=255)
                                                                                                                    ccGCTACCTTCACCTTTTCGCTGGCGGGA‑GGGGGCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGCGCAATGGGCGG‑ACCGGGGGTTTCGACGATAACTTGCACCAGGTGATCGACCGTCTGACAACGCACTACAAtt                                  >  1:328263/1‑150 (MQ=255)
                                                                                                                     cGCTACCTTCACCTTTTCGCTGGCGGGA‑GGGGTCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGCGCAATGGGCGT‑ACCGGGGTTTTCGACGATAACTTGCACCAGGTGATCGACCGTCTGACAGCGCACTACAATTg                                 <  1:1360902/150‑1 (MQ=255)
                                                                                                                     cGCTACCTTCACCTTTTCGCTGGCGGGA‑GGGGGCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGCGCAATGGGCGT‑ACCGGGGTTTTCGACGATAACTTGCACCAGGTGATCGACCGTCTGACAGCGCACTACAATTg                                 >  1:1838799/1‑150 (MQ=255)
                                                                                                                      gCTACCTTCACCTTTTCGCTGGCGGGG‑GGGGGCAACGCGATAGAAACGGTCAAACAATCGCGGGAAATGGTCGGGCGCCAAGGGGGTAACCGGGGTTTT‑GGCGGTAACTTTCCCCCGGGGGTCGCCCCTCCTACAACGCACCACaaattt                                >  2:4272834/1‑146 (MQ=255)
                                                                                                                      gCTACCTTCACCTTTTCGCTGGCGGGA‑GGGGTCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGCGCAATGGGCGT‑ACCGGGGTTTTCGACGATAACTTGCACCAGGTGATCGACCGTCTGACAGCGCACTACAATTGt                                <  2:722708/150‑1 (MQ=255)
                                                                                                                        tACCTTCACCTTTTCGCTGGCGGGG‑GGGGGCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGCGCAATGGGCGT‑ACCGGGGGGTTCGACGATAACTTGCACCAGGTGATCGACCGTCTGACAGCGCACTACAATTGtct                              >  2:497189/1‑150 (MQ=255)
                                                                                                                          ccTTCACCTTTTCGCTGGCGGGA‑GGGGTCAACGCGATAGAAACGGTCAAACAATCCCGGTAAGTGCTCGGGCGCAATGGGCGT‑ACCGGGGGTTTCGACGATAACTTGCACCAGGGGATCGACCGCCTGTCAGGGCACTACAATTGtctct                            >  2:3273429/1‑150 (MQ=255)
                                                                                                                           cTTCACCTTTTCGCTGGCGGGG‑GGGGGCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGGGCTCGGGCGCAATGGGCGT‑ACCGGGGGTTTCGACGATAAATTGCACCAGGGGATCGACCGTCTGACAGCGCCCTACAATTGtctctc                           >  2:2083471/1‑150 (MQ=255)
                                                                                                                           cTTCACCTTTTCGCTGGCGGGA‑GGGGTCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGCGCAATGGGCGT‑ACCGGGGTTTTCGACGATAACTTGCACCAGGTGATCGACCGTCTGACAGCGCACTACAATTGtctctc                           <  2:2727379/150‑1 (MQ=255)
                                                                                                                              cACCTTTTCGCTGGCGGGA‑GGGGTCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGCGCAATGGGCGT‑ACCGGGGTTTTCGACGATAACTTGCACCAGGTGATCGACCGTCTGACAGCGCACTACAATTGTCTCTCCgg                        <  1:3637054/150‑1 (MQ=255)
                                                                                                                               aCCTTTTCGCTGGCGGGA‑GGGGGCAACGCGATAGAAAAGGTCAAACAATCGCGGTAAGTGCTCGGGCGCAATGGGCGT‑ACCGGGGGTTTTGACGATAACTTGCACCAGGGGATCGACCGTCTGACAGCGCACTACAAATGTCTCTCCCGt                       >  2:1230138/1‑150 (MQ=255)
                                                                                                                                ccTTTTCGCTGGCGGGA‑GGGGTCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGCGCAATGGGCGT‑ACCGGGGTTTTCGACGATAACTTGCACCAGGTGATCGACCGTCTGACAGCGCACTACAATTGTCTCTCCGGtt                      <  2:3333778/150‑1 (MQ=255)
                                                                                                                                ccTTTTCGCTGGCGGCA‑GGGGTCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGCGCAATGGGCGT‑ACCGGGGTTTTCGACGATAACTTGCACCAGGTGATCGACCGTCTGACAGCGCACTACAATTGTCTCTCCGGtt                      <  2:3508679/150‑1 (MQ=255)
                                                                                                                                      cGCTGGCGGGA‑GGGGTCAACGCGATAGAAACGGTCAAACAATCGCGGGAAGTGCTCGGGCGCAATGGGCGT‑ACCGGGGGTTTCGACGATAACTTGCACCAGGGGATCGACCGTCTGACAGCGCACTACAATTGTCTCTCCGGTTGGCGta                >  2:2190502/1‑150 (MQ=255)
                                                                                                                                        cTGGCGGGA‑GGGGTCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGCGCAATGGGCGT‑ACCGGGGTTTTCGACGATAACTTGCACCAGGTGATCGACCGTCTGACAGCGCACTACAATTGTCTCTCCGGTTGGCGtata              <  1:2624291/150‑1 (MQ=255)
                                                                                                                                         tGGCGGGA‑GGGGTCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGCGCTATGGGCGT‑ACCGGGGTTTTCGACGATAACTTGCACCAGGTGATCGACCGTCTGACAGCGCACTACAATTGTCTCTCCGGTTGGCGTATaa             <  2:3542494/150‑1 (MQ=255)
                                                                                                                                         tGGCGGGA‑GGGGTCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGCGCAATGGGCGT‑ACCGGGGTTTTCGACGATAACTTGCACCAGGTGATCGACCGTCTGACAGCGCACTACAATTGTCTCTCCGGTTGGCGTATaa             >  1:2569136/1‑150 (MQ=255)
                                                                                                                                            cGGGA‑GGGGTCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGCGCAATGGGCGT‑ACCGGGGTTTTCGACGATAACTTGCACCAGGTGATCGACCGTCTGACAGCGCACTACAATTGTCTCTCCGGTTGGCGTATAACGc          <  1:18764/150‑1 (MQ=255)
                                                                                                                                             gggA‑GGGGTCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGCGCAATGGGCGT‑ACCGGGGTTTTCGACGATAACTTGCACCAGGTGATCGACCGTCTGACAGCGCACTACAATTGTCTCTCCGGTTGGCGTATAACGCa         >  2:3773047/1‑150 (MQ=255)
                                                                                                                                                a‑GGGGTCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGCGCAATGGGCGT‑ACCGGGGTTTTCGACGATAACTTGCACCAGGTGATCGACCGTCTGACAGCGCACTACAATTGTCTCTCCGGTTGGCGTATAACGCAggg      >  2:916783/1‑150 (MQ=255)
                                                                                                                                                a‑GGGGTCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGCGCAATGGGCGT‑ACCGGGGTTTTCGACGATAACTTGCACCAGGTGATCGACCGTCTGACAGCGCACTACAATTGTCTCTCCGGTTGGCGTATAACGCAggg      <  2:3043146/150‑1 (MQ=255)
                                                                                                                                                   gggTCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGCGCAATGGGCGT‑ACCGGGGTTTTCGACGATAACTTGCACCAGGTGATCGACCGTCTGACAGCGCACTACAATTGTCTCTCCGGTTGGCGTATAACGCAGGGCg    >  2:4018711/1‑150 (MQ=255)
                                                                                                                                                      |                                                                                                                                                     
TGCTGAAGGATTAAGCGGGTAATGTGATAACAAACCTTGTCCCCCGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGA‑GGGGTCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGCGCAATGGGCGT‑ACCGGGGTTTTCGACGATAACTTGCACCAGGTGATCGACCGTCTGACAGCGCACTACAATTGTCTCTCCGGTTGGCGTATAACGCAGGGCGTT  >  AP012306/472719‑473016

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑