Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123063,182,8610AC29.8% 309.2 / 24.9 131L198F (TTA→TTC) yiaNpredicted transporter
Reads supporting (aligned to +/- strand):  ref base A (79/13);  new base C (0/39);  total (79/52)
Fisher's exact test for biased strand distribution p-value = 1.90e-22
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 198 total aligned reads displayed.

ATTATTCCCCCTTCCATTCCGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGAT‑GCTTACCTGGTGGTGGCAGGCCAGTCG‑CCTTAATTT‑ACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTG‑TTTACCCCTACCGAAGCCGGGGCCGTTGCTGCTTTCTATGC  >  AP012306/3182712‑3183010
                                                                                                                                                        |                                                                                                                                                      
attattCCCCCTTCCATTCCGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGAT‑GCTTACCTGGTGGTGGCAGGCCAGTCG‑CCTTAATTT‑a                                                                                                                                                        <  1:3651456/150‑1 (MQ=255)
 ttattCCCCCTTCCATTCCGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGAT‑GCTTACCTGGTGGTGGCAGGCCAGTCG‑CCTTAATTT‑Ac                                                                                                                                                       <  2:3536643/150‑1 (MQ=255)
       ccccTTCCATTCCGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCATTTCCCCCCGCATGATGATTGGGGCAACGCTGGT‑GCTTACCTGGTGGGGGGAGGCCAGACGCCCTTATTTT‑Tcccccc                                                                                                                                                  >  2:3238211/1‑148 (MQ=255)
       ccccTTCCACTCCGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCGTTGCCCCCGGCCTGGTGATGGGGGCAACGCTGAT‑GCTTACCTGGTGGTGGGAGGCCAGACGCCCTTATTTT‑Tcccccc                                                                                                                                                  >  2:85701/1‑148 (MQ=255)
           ttCCATTCCGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGAT‑GCTTACCTGGTGGTGGCAGGCCAGTCG‑CCTTAATTT‑ACCCCGCCAGCa                                                                                                                                             <  1:1536280/150‑1 (MQ=255)
              cATTCCGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGGTTTGCCCCCTGCATGATGATGGGGGCAACGCTGTT‑GCTTACCTGGTGGTGGGAGGGCAGTCG‑CCTTTATTT‑TTCCCCCCCGCaaaa                                                                                                                                          >  2:766771/1‑150 (MQ=255)
               aTTCCGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCATTGCCCCGGGCATGATGATGGGCGCAACGCTGAT‑GCTTACCTGGTGGGGGCAGGCCAGTCG‑CCTTAATTT‑CCCCCGCCAGCaaaaa                                                                                                                                         <  2:148286/150‑1 (MQ=255)
                  ccGTTTATTATCTTCGGCGTTTCCAGGGGATTATCCATCAGTAAGCTGTTTATGGCGGGCATTGCCCCTGGCATGATGAGGGGCGCAACGCTGAT‑GCTTCCCTGGTGGTGGCAGGCCAGTCG‑CCTTAATTT‑ACCCCGCCAGCAAAAAGCa                                                                                                                                      <  1:1438940/150‑1 (MQ=255)
                   cGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCATTTCCCCCGGCATGGTGATGGGGGCCACGCTGAT‑GCTTTCCTGGTGGTGGGGGGGCAGGCG‑CCTTAATTT‑ACCCCGCCCCCAAAAAACaa                                                                                                                                     >  2:2408319/1‑150 (MQ=255)
                     tttATTATCTGGGGCGTTTCGGGGGGATTCTCCATGAGTAGGCTGTTTAGGGCGGGCATGGCCGCTGGGATGATGGGGGGCGCAGCGCTGAT‑GTTTCCGGGGGGGGGGCGGGCCAGTCG‑CTTTAATTT‑ACCCCGCCAGCAAAAAGCAACg                                                                                                                                   <  2:3098353/150‑1 (MQ=255)
                      ttattaTCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCATTTCCCCCCGCGTGATGATGGGGGCAACGCTGAT‑GCTTTCCTGGGGGGGGGAGGGCCGTCG‑CCCTTATTT‑TTCCCCCCCCCaaaaaaaaaaaa                                                                                                                                  >  2:3035184/1‑143 (MQ=255)
                       tattatCTTCGGCTTTTCCAGCGGATTATCCACCAGTAAGCTTTTTAGGGCGGGCATTCCCCCTGGCATGATGATGGGCGCAACGCTGAT‑GCTTCCCTGGTGGGGGCAGGCCAGTCG‑CTTTAATTT‑CCCCCGCCAGCAAAAAGCAACGAt                                                                                                                                 <  2:1087527/150‑1 (MQ=255)
                       tattatCTTCGGCTTTCCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCATTGCCCCTGGCATGATGAGGGGCGCAACGCTGAT‑GCTTCCCTGGTGGTGGCAGGCCAGTCG‑CCTTAATTT‑CCCCCGCCAGCAAAAAGCAACGAt                                                                                                                                 <  1:553889/150‑1 (MQ=255)
                          tatCTGCGGCTTTCCCGGGGGATTACCCATCAGTAAGCTGTTTAGGGCGGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGAT‑CCTTACCGGGGGGGGGCAGCCCAGCCG‑CTTTATTTT‑CCCCCGCCAGCAAAAAGCAACGATGCa                                                                                                                              <  2:2015127/150‑1 (MQ=255)
                            tCTTCGGTGTGCCCGGCGGATTATCCTTCAGTAGGCTTTTTTGGGCGGGCTTTGCCCCTGGAAGGATGGTGGCCGCAACCGTGTT‑GCTTACGTGGGGGGGGGGGGCCAGTCG‑CTTTAATTT‑ACCCCGCCAGCAAAAAGCAACGATGCAgg                                                                                                                            <  2:3995359/150‑1 (MQ=255)
                              ttCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCATTGCCCCCGGCATGATGATGGGCGCAACGCTGAT‑GCTTTCCTGGTGGGGGGGGGGCAGGCG‑CCTTTATTT‑AACCCCCCCGCAAAAAACAACGATGCAGGaa                                                                                                                          >  2:2917209/1‑150 (MQ=255)
                                cGGCGTTTCCAGCGGATTACCCATCAGTAAGCTGTTTATGGCGGGCATTGCCCCGGGCATGATGATGGGCGCAACGCTGAT‑GCTTCCCTGGTGGTGGCGGGCCAGTCG‑CCTTAATTT‑CCCCCGCCAGCAAAAAGCAACGATGCAGGAAAt                                                                                                                        <  2:2827173/150‑1 (MQ=255)
                                 ggCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCCTTGCCCCCGGCCTGTTGTTGGGGGCAACGCTGGT‑GCTTACCTGGTGGTGGGGGGCGCGACG‑CCTTTATTT‑ACCCCCCCCCCAAAAAAAAACGAGGAACGaaagt                                                                                                                       >  2:2499555/1‑148 (MQ=255)
                                      ttCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCAATTTCCCCGGCCCGATGATGGGCGCGACACCGAT‑GATTTCCTGGTGGGGGCAGGCCAGTCG‑CCCTAATTT‑TTCCCCCCCCCAAAAAACAACGATACAGGAAAACTGGcc                                                                                                                  >  2:4102727/1‑149 (MQ=255)
                                       tCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGGATTGCCCCCGGCGTGATGATGGGGGCAAAGCTGGT‑GCTTTCCTTGTGGGGGGGGGCCCGTCG‑CCTTAATTT‑TCCCCCCCAACCAAAAACAAAGAAGCAGGAAAACttggcg                                                                                                                 >  2:1502444/1‑145 (MQ=255)
                                         cAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGGATTGGCCCTGGGAGGATGAAGGGGGGAAAGCTGAG‑GGTTACCTGGGGGGGGGAGGGGGGGGG‑GCTTAATTT‑TACCCGCCCGCAAAAAAAAAAGATGGCGGAAAAATGGGActc                                                                                                               >  2:2429940/1‑150 (MQ=255)
                                          aGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCATTGCCCCCGGCATGATGATGGGGGCAACGCTGAT‑GGTTACCTGGTGGGGGGAGGCCCGGCG‑CCTTAATTT‑TCCCCCCCAGCAAAAAACAAAGATGCAGGAAATCTGGGActct                                                                                                              >  2:1741159/1‑150 (MQ=255)
                                             ggATTACCCATCAGGAAGCTGTTTAGGGGGGGCTTTCCCCCTGGCATGATGATGGGCCCAACGCTGAT‑GCTTACGTGGTGGTGGAAGGCCAGTCC‑CCTTAATTT‑CCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCtttt                                                                                                           <  2:415183/150‑1 (MQ=255)
                                               aTTATCCATCAGTAAGCTGTTTATGGCGGGGATTGCCCCCGGCATGATGATTGGCGCAAAGCTGAT‑GCTTACCTTGTGGGGGCAGGCCAGGCG‑CCCTAATTT‑ACCCCGCCCGCCAAAAACAACCAAGCAGGAAAACTTGCACTCtttttt                                                                                                         >  2:227717/1‑148 (MQ=255)
                                               aTTATCCAACAGTAAGCTGTTTATGGCGGGAATTGCCCCTGGCTTGATGATGGGGGCAACGCTGAT‑GCTTACCTGGTGGTGGCAGGCCAGTCG‑CTTTATTTT‑ACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGt                                                                                                         <  2:2437195/150‑1 (MQ=255)
                                               aTTACCCAGCAGTAGCCTGTTTATGGCGGTCTTTGCCCCTGCCATGATGAGGGGCTCATCGC‑GATGGCTTACCGGGGGGGGGCAGGCCGGTCG‑CTTTAATTT‑CCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGt                                                                                                         <  2:2803755/150‑1 (MQ=255)
                                                ttATCCATCAGTAAGCTGTTTATGGCGGGCATTTCCCCCGGCATGATGATTGGGGCAACGCTGAT‑GCTTTCCTGGTGGGGGCAGGCCAGTCG‑CCCTAATTT‑TCCCCCCCAGCAAAAAACAACGATGCAGGAAAACTGGCACTCttttttt                                                                                                        >  2:2528875/1‑147 (MQ=255)
                                                 tacccATCAGTAAGCTGTTTATGGCGGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGAT‑GCTTCCCTGGTGGTGGCAGGCCAGTCG‑CTTTAATTT‑ACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGtct                                                                                                       <  1:3736377/147‑1 (MQ=255)
                                                 tATCCATCAGTAAGCTGTTTATGGCGGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGAT‑GCTTACCTGGTGGTGGCAGGCCAGTCG‑CCTTAATTT‑CCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGtct                                                                                                       <  1:2375945/150‑1 (MQ=255)
                                                 tATCCATCAGTAAGCTGTTTATGGCGGGCATTGCCCCCGGCATGATGATGGGGGCAACGCTGAT‑GCTTTCCTGGTGGGGGGAGGCCAGTCG‑CCTTTATTT‑TTCCCCCCCGCAAAAAACAACGATGCAGGGAATCTGGCACTCTTTTTtct                                                                                                       >  2:591512/1‑150 (MQ=255)
                                                        cAGTAAGTGTTTTATGGGGGCCATGCCCCCGGGCAGGATGAGGGGCGAACCGCTGAT‑GCTTACCTGGTGGGGGCGGCCCAGTCG‑CTTTAATTT‑CCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTAt                                                                                                <  2:870861/150‑1 (MQ=255)
                                                        cAGTAAGCTGTTTATGGCGGGCATTGCCCCCGGCATGATGATGGGGGCAACGCTGAT‑TGTTTCCTTGTGGGGGGAGGCCCGTCG‑CCTTTATTT‑TACCCCCCCGCAAAAAACAACGATGGAGGGAAACTGGCACTCTTTTTTTTCCGGTAt                                                                                                >  2:3805977/1‑150 (MQ=255)
                                                             agttGTTTATGGCGGGCATGCCCCCTGGAATGATGAGGGGCACAACGCTGAT‑CCTTCCCTGGTGGTGGAAGGCAAGTCG‑CCTTAATTT‑CCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTggg                                                                                           <  2:2236846/147‑1 (MQ=255)
                                                              cctttttAGGGCGGGCATTGCCCCTGGCATGATGAGGGGCGCAACGCTGAT‑GCTTACTTGGTGGTGGCAGGCCAGTCG‑CCTTAATTT‑CCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGgc                                                                                          <  2:1787624/146‑1 (MQ=255)
                                                                  ttttTGGCCCGCTTTCCCCCTGCCATGATCATGGGCTCAGCGCTGAT‑CTTTCCCTGGTGGTGGCCGCCCCGCCC‑CCTTTATTC‑CCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTg                                                                                      <  2:4133175/150‑1 (MQ=255)
                                                                  tttGTGGCGGGCTTTGCCCCTGGCATGATGATGGCCGCAACGCTGAG‑GCTTACCTGGTGGTGGCGGGCCAGTCG‑CTTTAATTT‑CCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTg                                                                                      <  2:2671307/150‑1 (MQ=255)
                                                                     aTGGCGGGCATTGCCCTGGGCATGATGATGGGCGCAACGCTGAT‑GCTTACCTGGTGGTGGCAGGCCAGTCG‑CCTTAATTT‑ACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGttt                                                                                   <  2:1822931/150‑1 (MQ=255)
                                                                       ggCGGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGAT‑GCTTACCTGGTGGTGGCAGGCCAGTCG‑CCTTAATTT‑ACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGACGCTGTttct                                                                                 >  1:4194964/1‑150 (MQ=255)
                                                                        gCGGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGAT‑GCTTACCTGGTGGTGGCAGGCCAGTCG‑CCTTAATTT‑ACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTttctt                                                                                >  2:3648092/1‑150 (MQ=255)
                                                                          gggCTTTGCCCCTGGCATGATGATGGGCGAAACGTGGAT‑GCTTCCCTGGTGGTGGCAGGCCAGTCG‑CTTTATTTT‑ACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTcc                                                                              <  2:902512/150‑1 (MQ=255)
                                                                          gggCATTGCCCCTGGCATGATGATGGGCGCAACGCTGAT‑GCTTACCTGGTGGTGGCAGGCCAGTCG‑CCTTAATTT‑ACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTcc                                                                              >  1:2328551/1‑150 (MQ=255)
                                                                            ccatgcccccTGGCATGATGGTGGGCCCAACGCTGGT‑GCTTCCCTGGTGGGGGCAGGCGAGTCG‑CTTTAATTT‑CCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAg                                                                            <  2:2940140/144‑1 (MQ=255)
                                                                             cATTGCCCCTGGCATGATGATGGGCGCAACGCTGAT‑GCTTACCTGGTGGTGGCAGGCCAGTCG‑CCTTAATTT‑ACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGt                                                                           >  2:1029378/1‑150 (MQ=255)
                                                                             cATTGCCCCTGGCATGATGATGGGCGCAACGCTGAT‑GCTTACCTGGTGGTGGCAGGCCAGTCG‑CCTTAATTT‑ACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGt                                                                           >  1:1689956/1‑150 (MQ=255)
                                                                                 gCCCCTGGCATGATGATGGGCGCAACGCTGAT‑GCTTACCTGGTGGTGGCAGGCCAGTCG‑CCTTAATTT‑ACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGtcatc                                                                       >  2:824748/1‑150 (MQ=255)
                                                                                 gCCCCTGGCATGAGGGGGGGCGCAACGCTGAT‑GCTTCCCTGGTGGGGGCGGGCCGGTCG‑CCTTAATTT‑CCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGtcatc                                                                       <  2:610180/150‑1 (MQ=255)
                                                                                   cccTGGCATGATGATGGGCGCAACGCTGAT‑GCTTACCTGGTGGTGGCAGGCCAGTCG‑CCTTAATTT‑ACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTcatcat                                                                     >  1:617364/1‑150 (MQ=255)
                                                                                   cccTGGCATGATGATGGGCGCAACGCTGAT‑GCTTACCTGGTGGTGGCAGGCCAGTCG‑CCTTAATTT‑ACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTcatcat                                                                     >  2:2183120/1‑150 (MQ=255)
                                                                                    ccTGGCATGATGATGGGCGAACCGCTGAT‑GCTTACCTGGTGGTGGCAGGCCAGTCG‑CTTTAATTT‑CCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCatt                                                                    <  1:1272181/150‑1 (MQ=255)
                                                                                       ggCATGATGATGGGCGCAACGCTGAT‑GCTTACCTGGTGGTGGCAGGCCAGTCG‑CCTTAATTT‑ACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCattatt                                                                 >  1:4129546/1‑150 (MQ=255)
                                                                                       ggCATGATGATGGGCGCAACGCTGAT‑GCTTACCTGGTGGTGGCAGGCCAGTCG‑CCTTAATTT‑ACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCattatt                                                                 >  1:1648940/1‑150 (MQ=255)
                                                                                        gCATGATGAGGGGCGCAACGCTGAT‑GCTTCCCTGGTGGTGGCAGGCCAGTCG‑CCTTAATTT‑CCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTg                                                                <  1:3466529/150‑1 (MQ=255)
                                                                                           tgatgatgGGCGCAACGCTGAT‑GCTTACCTGGTGGTGGCAGGCCAGTCG‑CCTTAATTT‑ACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCg                                                             >  2:39789/1‑150 (MQ=255)
                                                                                            gatgatgGGCGCAACGCTGAT‑GCTTACCTGGTGGTGGCAGGCCAGTCG‑CCTTAATTT‑ACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCgg                                                            >  1:3288397/1‑150 (MQ=255)
                                                                                             atgatgGGCGCAACGCTGAT‑GCTTACCTGGTGGTGGCAGGCCAGTCG‑CCTTAATTT‑ACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCggg                                                           >  1:2302028/1‑150 (MQ=255)
                                                                                             agaatgGGCGCAGCGCTGAG‑GCTTACCTGGGGGTGGCAGGCCAGCCG‑CCTTAATTT‑TCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCggg                                                           <  1:4052099/147‑1 (MQ=255)
                                                                                              tgatgGGCGCAACGCTGAT‑GCTTACCTGGTGGTGGCAGGCCAGTCG‑CCTTAATTT‑CCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGt                                                          <  1:3363946/150‑1 (MQ=255)
                                                                                              tgatgGGCGCAACGCTGAT‑GCTTACCTGGTGGTGGCAGGCCAGTCG‑CCTTAATTT‑ACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCgggg                                                          >  1:1742909/1‑149 (MQ=255)
                                                                                                atgGGCGCAACGCTGAT‑GCTTACCTGGTGGTGGCAGGCCAGTCG‑CCTTAATTT‑ACCCCGCCAGCGAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGgtt                                                        >  2:1696464/1‑150 (MQ=255)
                                                                                                atgGGCGCAACGCTGAT‑GCTTACCTGGTGGTGGCAGGCCAGTCG‑CCTTAATTT‑ACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGttt                                                        >  2:3561961/1‑150 (MQ=255)
                                                                                                atgGGCGCAACGCTGAT‑GCTTACCTGGTGGTGGCAGGCCAGTCG‑CCTTAATTT‑ACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGgtt                                                        >  2:3912546/1‑150 (MQ=255)
                                                                                                 tgGGCGCAACGCTGAT‑GCTTACCTGGTGGTGGCAGGCCAGTCG‑CCTTAATTT‑ACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTc                                                       >  1:2936470/1‑150 (MQ=255)
                                                                                                  gggCGCAACGCTGAT‑GCTTACCTGGTGGTGGCAGGCCAGTCG‑CTTTAATTT‑CCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCg                                                      <  1:726756/150‑1 (MQ=255)
                                                                                                  gggCGCAACGCTGAT‑GCTTACCTGGTGGTGGCAGGCCAGTCG‑CCTTAATTT‑ACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGgtttg                                                      >  2:848399/1‑148 (MQ=255)
                                                                                                     cgcAACGCTGAT‑GCTTACCTGGTGGTGGCAGGCCAGTCG‑CCTTAATTT‑ACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGTCGGAGTTTGCTc                                                   >  1:2270801/1‑150 (MQ=255)
                                                                                                     cgcAACGCTGAT‑GCTTACCTGGTGGTGGCAGGCCAGTCG‑CCTTAATTT‑ACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGGTtctctc                                                   >  2:598342/1‑150 (MQ=255)
                                                                                                      gcAACGCTGAT‑GCTTACCTGGTGGTGGCAGGCCAGTCG‑CCTTAATTT‑ACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGGTTTGCTcc                                                  >  1:699361/1‑150 (MQ=255)
                                                                                                       cAACGCTGAT‑GCTTCCCTGGTGGTGGCAGGCCAGTCG‑CTTTATTTT‑CCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCg                                                 <  1:3486101/150‑1 (MQ=255)
                                                                                                       cAACGCTGAT‑GCTTACCTGGTGGTGGCAGGCCAGTCG‑CCTTAATTT‑ACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCg                                                 >  1:3858272/1‑150 (MQ=255)
                                                                                                       cAACGCTGAT‑GCTTACCTGGTGGTGGCAGGCCAGTCG‑CCTTAATTT‑ACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGGTTTGCTCCg                                                 >  1:3910011/1‑150 (MQ=255)
                                                                                                       aaaCGTTGAT‑TCTTCCGTGGTGTCGGCAGGCCGGTCG‑CTTTAATTG‑CCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCg                                                 <  2:2144089/149‑1 (MQ=255)
                                                                                                        aaCGCTGAT‑GCTTACCTGGTGGTGGCAGGCCAGTCG‑CCTTAATTT‑ACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGGTTTGTTcccg                                                >  2:2769971/1‑148 (MQ=255)
                                                                                                         aCGCTGAT‑GCTTACCTGGTGGTGGCAGGCCAGTCG‑CCTTAATTT‑ACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGGTTTGCTCCCGc                                               >  2:187565/1‑150 (MQ=255)
                                                                                                           gCTGAT‑GCTTACCTGGTGGTGGCAGGCCAGTCG‑CCTTAATTT‑ACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGccc                                             >  1:3428301/1‑149 (MQ=255)
                                                                                                           gCTGAT‑GCTTACCTGGTGGTGGCAGGCCAGTCG‑CCTTAATTT‑ACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGGTTTGCTcccggct                                             >  1:659130/1‑145 (MQ=255)
                                                                                                           gCTGAT‑GCTTACCTGGTGGTGGCAGGCCAGTCG‑CCTTAATTT‑ACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGGTTCGCTCCCGccc                                             >  1:2566786/1‑149 (MQ=255)
                                                                                                             tGAT‑GCTTCCCGGGTGGGGGCGGGCCAGTCG‑CTTTAATTT‑CCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTG‑t                                          <  1:2046986/150‑1 (MQ=255)
                                                                                                             tGAT‑GCTTACCTGGTGGTGGCAGGCCAGTCG‑CCTTAATTT‑ACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCTTGCCGGCCCG‑t                                          >  2:955566/1‑150 (MQ=255)
                                                                                                              gAT‑GCTTCCCGGGGGGTGGCAGGCCGTTCG‑CTTTAATTT‑CCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTG‑tt                                         <  2:1774425/150‑1 (MQ=255)
                                                                                                               aT‑GCTTACCTGGTGGTGGCAGGCCAGTCG‑CCTTAATTT‑CCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTG‑ttt                                        <  1:2601280/150‑1 (MQ=255)
                                                                                                               aT‑GCTTACCTGGTGGTGGCAGGCCAGTCG‑CCTTAATTT‑ACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTTGGTCCGCCCCG‑ttt                                        >  2:1577088/1‑150 (MQ=255)
                                                                                                               aT‑GCTTACCTGGTGGTGGCAGGCCAGTCG‑CCTTAATTT‑ACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTTCCTCCCGCCCG‑ttt                                        >  1:1053817/1‑150 (MQ=255)
                                                                                                                t‑GCTTACCTGGTGGTGGCAGGCCAGTCG‑CCTTAATTT‑ACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGGTTTTCTCCGGCCCG‑tttt                                       >  2:3610749/1‑149 (MQ=255)
                                                                                                                 gccgtACCTGGGGGTGGCAGCCCAGTCG‑CTTAAATTA‑ACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTG‑TTTa                                       <  2:3179207/146‑1 (MQ=255)
                                                                                                                   cTTACCTGGTGGTGGCAGGCCAGTCG‑CCTTAATTT‑ACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGGTTTTCTCCCGCCTC‑TTTTcc                                     >  2:3352229/1‑150 (MQ=255)
                                                                                                                    ttACCTGGTGGTGGCAGGCCAGTCG‑CCTTAATTT‑ACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTG‑TTTAccc                                    <  1:93314/150‑1 (MQ=255)
                                                                                                                     tACCTGGTGGTGGCAGGCCAGTCG‑CCTTAATTT‑ACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGGTTTGCTCCCGCCCG‑tttttccc                                   >  2:3192149/1‑145 (MQ=255)
                                                                                                                      cccTGGTGGTGGCAGGCCAGTCG‑CCTTAATTT‑CCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTG‑TTTACCCCt                                  <  1:1147637/149‑1 (MQ=255)
                                                                                                                       ccTGGTGGTGGCAGGCCAGTCG‑CCTTAATTT‑ACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGATTTTGCTCCGGCCCG‑TTTACCCCTa                                 >  1:2320348/1‑150 (MQ=255)
                                                                                                                          ggtggtggCAGGCCAGTCG‑CCTTAATTT‑ACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGGTTTGCTCCGGGCTG‑TTTATCCCCACCg                              >  1:2913550/1‑150 (MQ=255)
                                                                                                                              gtggCAGGCCAGTCG‑CCTTAATTT‑ACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTATCGCTCCCGCCCC‑TTTTCCCCCTCCGAAGc                          >  1:3313039/1‑150 (MQ=255)
                                                                                                                                   aGGCCAGTCG‑CCTTAATTT‑ACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGGTTTTCTCCCGGCCG‑TTTTTCCCCACCCAAACCcggg                     >  1:2990811/1‑150 (MQ=255)
                                                                                                                                    ggCCAGTCC‑CCTAAATTT‑CCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTG‑TTTACCCCTACCGAAGCCGGGGc                    <  1:3504008/150‑1 (MQ=255)
                                                                                                                                       cccttccccTTAATTTCCCCCCGCCAGAAAAAAGCAACGAGGCAGAAAATCGGGCCCTCTTTTGTCTCCGGTATCTGGGCGCTTTTTCTTCCAGTCATCATTATTGGGGGGTTTCGCTCCGGCCTG‑TTTCCCCCTACCGAAGCCGGGGcc                   <  2:2034706/143‑1 (MQ=255)
                                                                                                                                           cG‑CCTTAATTT‑ACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCCGCCTG‑TTTTCCCCCCCCCAAGCCCGGGCCgctgct             >  1:108370/1‑150 (MQ=255)
                                                                                                                                                ttAATTT‑ACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGGTTTGCTCCCGCCTG‑TTTTCCCCCACCCAAGCCCGGGGCGCTGCTGCtt         >  1:2720544/1‑150 (MQ=255)
                                                                                                                                                    ttt‑ACCCAGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTG‑TTTACCCCTACCGAAGCCGGGGCCGTTGCTGCTTTCTa     <  2:193870/150‑1 (MQ=255)
                                                                                                                                                     tt‑ACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGGTTGCCGCCGGCCCGTTTTACCCCT‑CCGAAACCCGGGCCGGTGCTGCttttttt    >  1:635577/1‑146 (MQ=255)
                                                                                                                                                      t‑ACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTG‑TTTACCCCTACCGAAGCCGGGGCCGTTGCTGCTTTCTATg   <  2:2570543/150‑1 (MQ=255)
                                                                                                                                                        aCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGCATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGGTTTTCTCCGGCCCGTTTTTCCCCTCCCCAA‑CCGGGGGCGGTGCGGttttttttgt  >  2:1779069/1‑144 (MQ=255)
                                                                                                                                                        |                                                                                                                                                      
ATTATTCCCCCTTCCATTCCGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGAT‑GCTTACCTGGTGGTGGCAGGCCAGTCG‑CCTTAATTT‑ACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTG‑TTTACCCCTACCGAAGCCGGGGCCGTTGCTGCTTTCTATGC  >  AP012306/3182712‑3183010

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑